JP2002037709A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin care preparation capable of activating the physiological function of the skin, therefore effective for preventing and ameliorating rough skin or skin aging. SOLUTION: This skin care preparation is obtained by formulating a skin care preparation base with at least one kind selected from extracts of yeast with high thioredoxin activity, wherein the yeast with high thioredoxin activity to be preferably used is a thioredoxin-rich strain isolated from Saccharomyces cerevisiae Meyer.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin which activates physiological functions of the skin and is effective for preventing and improving rough skin and aging of the skin. More specifically, the present invention relates to an external preparation for skin comprising one or more selected from yeast extracts having high thioredoxin activity.

[0002]

2. Description of the Related Art Yeast extract has a skin moisturizing effect and a skin cell activating effect, is a component widely used in external preparations for the skin, and has also been reported to have a hyaluronic acid production promoting effect (Japanese Patent Laid-Open No. Hei 8-8). 163983). However, sufficient action and effect cannot be obtained only by blending the yeast extract, and the blend is often blended with other physiologically active ingredients.

[0003] On the other hand, as it has been revealed that reactive oxygen species generated by various stresses such as ultraviolet rays and metabolism in skin tissues are involved in the occurrence and progression of skin function deterioration and aging, polyphenols such as tannins and flavonoids have been developed. Screening of components that eliminate reactive oxygen species, such as plant extracts and various plant extracts, has been actively conducted. However, since various factors besides reactive oxygen species are involved in the deterioration and aging of the skin due to ultraviolet rays and aging, simply erasing the reactive oxygen species is sufficient to reduce the skin function and aging. It was difficult to prevent or improve the situation.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a skin external preparation which can effectively prevent and improve the deterioration and aging of the skin due to ultraviolet rays or aging.

[0005]

As a result of various studies to solve the above problems, a strain having a high thioredoxin activity in yeast was found, and this extract was incorporated into an external preparation for skin. Chemical action is obtained,
The present invention has been completed.

That is, in the present invention, one or more selected from yeast extracts having high thioredoxin activity are contained in a skin external preparation base. It has been already disclosed that thioredoxin is a protein involved in redox control and has a function of eliminating reactive oxygen species (Japanese Patent Application Laid-Open No. 9-12471). However, when a yeast extract having a high thioredoxin activity was used, a skin function activating action that was unexpectedly exhibited was exhibited due to the combined effect with a component other than thioredoxin.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, a yeast extract having a high thioredoxin activity having a thioredoxin activity of 30 units / ml or more is used. As yeast having high thioredoxin activity used to obtain the extract,
A thioredoxin-rich strain isolated from Saccharomyces cerevisiae Meyer, a baker's yeast, is preferably used.

[0008] The yeast extract having a high thioredoxin activity used in the present invention includes an extract of the yeast with an aqueous solvent, a heat extract, and a yeast which has been lysed by autolysis, acid hydrolysis or enzymatic degradation and then filtered. Or a product obtained by drying the lysate and extracting it with an aqueous solvent.

As an extraction solvent, in addition to water, physiological saline,
Aqueous solvents such as a phosphate buffer and a phosphate buffered saline can be used, and one or two or more thereof are selected and used. The yeast may be dried and / or pulverized before being subjected to extraction, or may be homogenized in an extraction solvent or subjected to sonication. The extract can also be obtained by irradiating the medium with ultraviolet light. It is appropriate that the extraction temperature is set to a temperature from about 0 ° C. to the boiling point of the extraction solvent or lower.
The extraction time varies depending on the type of extraction solvent, the extraction temperature and the extraction method, but is preferably about 1 hour to 5 days.

The extract of yeast with the above-mentioned polar solvent can be contained in the external preparation for skin according to the present invention as it is. However, the extract is concentrated and dried, or the concentrated and dried product is dissolved again in an aqueous solvent. Alternatively, it may be used after purifying or dispersing such as decolorization, deodorization, and desalting within a range that does not impair the skin function activating action. For preservation, it can be freeze-dried after the purification treatment and dissolved in a solvent before use. Further, it may be contained in vesicles such as liposomes or microcapsules.

The yeast extract having a high thioredoxin activity may be prepared as described above, but may be prepared using commercially available materials such as "East Liquid TRX" sold by Ichimaru Falcos Co., Ltd. You may.
In the present invention, one or more of these yeast extracts are selected and used. The content per total amount of the external preparation for skin is not particularly limited, but from the physiological function and the bioavailability of the external preparation for skin, 0.0001 to
Suitably, it is about 10.0% by weight.

The external preparation for skin according to the present invention can be provided in the form of lotions, emulsions, gels, creams, ointments, powders, granules, powders and the like. Also, skin cosmetics such as lotions, emulsions, creams, packs, etc., makeup base lotions, makeup base creams, foundations such as emulsions, creams, ointments, powders, etc., makeups such as eye colors and cheek colors. , Hand creams, leg creams, body lotions and other body cosmetics.

The external preparation for skin according to the present invention includes, in addition to one or more yeast extracts having high thioredoxin activity, oils, surfactants, humectants, emollients, pigments, fragrances, and ultraviolet rays. Raw materials for general pharmaceuticals and cosmetics such as absorbers, ultraviolet light scattering agents, antioxidants, antibacterial and fungicides, active oxygen scavengers, anti-inflammatory agents, whitening agents, skin cell activators, anti-mutagenicity Physiologically active components such as a component having an action and a component having a function of suppressing a decrease in immune function due to ultraviolet rays can also be contained.

Further, the features of the present invention will be described in detail with reference to examples.

[0015]

First, a preparation example of a yeast extract having a high thioredoxin activity used in the present invention will be described.

[Thioredoxin highly active yeast extract 1] Sa
2 liters of purified water was added to 200 g of a dry powder of a thioredoxin-rich strain isolated from ccharomyces cerevisiae Meyer, and allowed to stand at room temperature for 24 hours to allow autolysis, followed by treatment at 60 ° C. to 80 ° C. for 4 hours. did. Then, it was filtered and sterilized with a membrane filter to obtain the title yeast extract.

[Thioredoxin highly active yeast extract 2] Sa
A thioredoxin-rich strain isolated from ccharomyces cerevisiae Meyer is cultured, and a lysate obtained by autolyzing 500 g of cells in purified water is filtered through a membrane filter, sterilized, concentrated, lyophilized, and then lyophilized. Immersed in 500 ml of phosphate buffered saline, and
After stirring and extracting for days, the filtrate was collected by filtration and used as the title yeast extract.

[Thioredoxin highly active yeast extract 3] Sa
A thioredoxin-rich strain isolated from ccharomyces cerevisiae Meyer is cultured, and a lysate obtained by acid-decomposing 500 g of cells is filtered and sterilized with a membrane filter, and the filtrate is concentrated and then dissolved in 500 ml of purified water. And the title yeast extract.

[Thioredoxin highly active yeast extract 4] Sa
Two liters of purified water was added to 200 g of the dry powder of the thioredoxin-rich strain isolated from ccharomyces cerevisiae Meyer, and the mixture was heated and extracted for 2 hours. After cooling, the extract was filtered and sterilized with a membrane filter, and the filtrate was freeze-dried to obtain the title yeast extract.

The results of quantification of the protein content and thioredoxin activity of the thioredoxin highly active yeast extract prepared above are shown in Table 1. In addition, about thioredoxin high activity yeast extract 4, 1.0 weight%
Quantification was performed on the aqueous solution. For comparison, a wild strain of Saccharomyces cerevisiae showing thioredoxin activity was also used.
Add 200 liters of purified water to 200 g of Meyer dry powder,
After standing at room temperature for 24 hours to allow autolysis, 60
The results of quantification of the extract obtained by treating at 4 ° C. to 80 ° C. for 4 hours and filtering and sterilizing with a membrane filter are also shown.

[0021]

[Table 1]

In Table 1, all the thioredoxin highly active yeast extracts contained 9.3 mg / ml or more of protein, and showed thioredoxin activity of 32.5 units / ml or more. On the other hand, in the yeast extract of the wild strain, the protein content was almost the same as that of the thioredoxin-rich extract, but the thioredoxin activity was 15.0 units / m2.
and １ ／ or less of the extract of the strain containing high thioredoxin.

Next, the formulations of the examples of the external preparation for skin according to the present invention are shown.

[Example 1] Lotion (1) Ethanol 20.00 (% by weight) (2) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.00 (3) Fragrance 0.10 (4) Paraoxy Methyl benzoate 0.10 (5) Dipropylene glycol 5.00 (6) 1,3-butylene glycol 10.00 (7) Thioredoxin highly active yeast extract 1 0.05 (8) Purified water 63.75 Production method: (2) to (4) are added to (1) and dissolved to form an alcohol phase. On the other hand, (5) to (7) are sequentially dissolved in (8) to form an aqueous phase. Add the alcohol phase to the aqueous phase, stir and mix.

Example 2 Oil-in-Water Emulsion (1) Oleyl oleate 5.0 (% by weight) (2) Dimethylpolysiloxane 3.0 (3) Vaseline 0.5 (4) Cetanol 1.0 ( 5) Sorbitan sesquioleate 0.8 (6) Polyoxyethylene (20E.O.) oleyl ether 1.2 (7) Tocopherol acetate 0.2 (8) Dipropylene glycol 6.0 (9) Hydroxyethyl cellulose 0 0.3 (10) Methyl paraoxybenzoate 0.1 (11) Purified water 78.8 (12) Ethanol 3.0 (13) Thioredoxin highly active yeast extract 2 0.1 Production method: (1) to (6) Mix the oil phase components, heat and dissolve to 70 ° C
And On the other hand, the aqueous phase components (8) to (11) were mixed and dissolved by heating to 70 ° C., and the oil phase was added thereto, emulsified by a homogenizer, cooled, and cooled to 40 ° C. at (12) and (13). ) Are sequentially added and mixed.

[Example 3] Oil-in-water cream (1) Beeswax 6.0 (wt%) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl Fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20E.O.) sorbitan 5.0 Monolaurate (8) Propylene glycol 5.0 (9) Paraoxy Methyl benzoate 0.1 (10) Purified water 37.0 (11) Highly active thioredoxin yeast extract 3 0.2 (12) Highly active thioredoxin yeast extract 4 0.2 Production method: (1) to (7) The oil phase components are mixed and dissolved to 75 ° C. On the other hand, the aqueous phase components (8) to (10)
Heat to ° C. Next, the oil phase component is added to the water phase component, preliminarily emulsified, and then uniformly emulsified by a homomixer. After cooling, (11) and (12) are added and mixed at 40 ° C.

Example 4 Water-in-Oil Sunscreen Cream (1) Liquid Paraffin 30.0 (% by weight) (2) Microcrystalline Wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl Diolein Acid ester 5.0 (5) sodium L-glutamate 1.6 (6) L-serine 0.4 (7) octyl paramethoxycinnamate 3.0 (8) oxybenzone 2.0 (9) 4-tert-butyl- 4'-methoxydibenzoylmethane 0.5 (10) Silicon-treated fine particle titanium oxide 3.0 (11) Propylene glycol 3.0 (12) Methyl parahydroxybenzoate 0.1 (13) Thioredoxin Highly active yeast extract 10 5.5 (14) Purified water 43.8 (15) Fragrance 0.1 Production method: (5), (6) was dissolved in a part of (14) to 50 ° C, and preliminarily heated to 50 ° C. ) Add slowly with stirring I do. This is mixed in advance and uniformly dispersed in (1) to (3) heated and dissolved at 70 ° C. Then, (7) to (10) are added and uniformly mixed and dispersed. A solution obtained by dissolving (11) to (13) in the remainder of (14) and heating to 70 ° C. is added thereto with stirring, and emulsified by a homomixer. After cooling, at 50 ° C
Add (15) and mix.

Example 5 Gel (1) Dipropylene glycol 10.0 (% by weight) (2) Carboxyvinyl polymer 0.5 (3) Potassium hydroxide 0.1 (4) Methyl paraoxybenzoate 1 (5) Purified water 88.6 (6) Thioredoxin highly active yeast extract 2 0.5 (7) Horse chestnut extract 0.2 Production method: After uniformly dissolving (2) in (5), (1) (4) is dissolved and added to), then (3) is added to increase the viscosity, and (6) and (7) are sequentially added and mixed. In addition, the horse chestnut extract is
250 g of horse chestnut fruit was dried, pulverized, immersed in a 50% by volume aqueous ethanol solution, extracted at 20 ° C. for 5 days, and filtered.

Example 6 Liposomal Agent (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25E.O.) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl parahydroxybenzoate 0.1 (6) Fragrance 0.1 (7) Purified water 79.6 (8) Thioredoxin highly active yeast extract 4 5.0 Encapsulated liposome Production method: ( 5) and (6) are dissolved in (4), added to (7) together with (1) to (3), mixed uniformly, and dispersed by adding (8).
The thioredoxin-encapsulated liposome of (8) is a 5.0% by weight aqueous solution of thioredoxin highly active yeast extract 4 in 10% by weight.
80 g of soybean lecithin was added to 0 ml, suspended at 55 ° C., then sonicated to prepare liposomes, and recovered by centrifugation to prepare.

Example 7 Oil-in-water emulsion ointment (1) White petrolatum 25.00 (% by weight) (2) Stearyl alcohol 25.00 (3) Glycerin 12.00 (4) Sodium lauryl sulfate 1.00 (5) Lipoic acid 0.02 (6) Methyl paraoxybenzoate 0.10 (7) Thioredoxin highly active yeast extract 3 0.15 (8) Purified water 36.73 Production method: Oil of (1) to (5) The phase components are mixed and heated to uniformly dissolve and brought to 75 ° C. On the other hand, the aqueous phase components (6) to (8) are mixed,
Heat to 75 ° C. The oil phase component is gradually added to the water phase component with stirring, emulsified, and cooled.

Example 8 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsification Glyceryl monostearic acid 2.0 ester (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Methyl paraoxybenzoate 0.1 (8) Purified water 68.4 (9) Titanium oxide 2 0.0 (10) Bengala 0.4 (11) Yellow iron oxide 0.1 (12) Highly active thioredoxin yeast extract 1 0.1 (13) Fragrance 0.1 Production method: oil phase of (1) to (4) Mix and dissolve the ingredients to 75 ° C. On the other hand, the aqueous phase components (5) to (8) are mixed and dissolved by heating, and the pigment components (9) to (11) are added thereto and uniformly dispersed by a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified by a homomixer. After cooling, (12) and (13) are added and mixed at 40 ° C.

Example 9 Emulsion Foundation for Whitening (1) Stearic Acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 ( 5) Decaglyceryl monoisopalmitate 9.00 (6) 1,3-butylene crychol 6.00 (7) Potassium hydroxide 0.08 (8) Methyl parahydroxybenzoate 0.10 (9) High activity of thioredoxin Yeast extract 2 0.01 (10) Purified water 53.51 (11) Titanium oxide 9.00 (12) Talc 7.40 (13) Bengala 0.50 (14) Yellow iron oxide 1.10 (15) Black Iron oxide 0.10 (16) Fragrance 0.15 (17) Knotweed extract 0.05 Production method: Mix and dissolve oil phase components (1) to (5) to 75 ° C. On the other hand, the aqueous phase components (6) to (10) were mixed and dissolved by heating.
The pigment components (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified by a homomixer. After cooling, (16) and (17) are added and mixed at 40 ° C. The knotweed extract of (17) was obtained by drying and pulverizing 250 g of knotweed root, immersing it in 1.5 liters of a 50% by volume aqueous ethanol solution at 20 ° C. for 7 days, and collecting the filtrate by filtration. Was.

Example 10 Water-in-oil Cream Foundation for Sunscreen (1) Liquid Paraffin 5.00 (% by weight) (2) Decamethylcyclopentasiloxane 12.00 (3) Polyoxyethylene-modified dimethylpoly 4.00 Siloxane (4) Octyl paramethoxycinnamate 5.00 (5) Oxybenzone 2.50 (6) 1,3-butylene glycol 5.00 (7) Polyoxyethylene (20E.O.) Sorbitan 2.00 Monolaurin Acid ester (8) Methyl parahydroxybenzoate 0.10 (9) Thioredoxin highly active yeast extract 2 0.02 (10) Purified water 44.28 (11) Sericite 5.36 (12) Kaolin 4.00 (13) ) Fine particle titanium oxide 9.32 (14) Bengala 0.36 (15) Yellow iron oxide 0.80 (16) Black iron oxide 0.16 (17) Fragrance 0.10 Production method: The aqueous phase components of (6) to (10) are mixed and heated to 70 ° C., and the powder components of (11) to (16), which have been mixed and ground in advance, are added, and the mixture is homogenized with a homomixer. To process. On the other hand, the oil phase components (1) to (5) are mixed, heated to 70 ° C., added, and homomixed at 70 ° C. Then, cool while stirring.
Add (17) at 5 ° C., cool to room temperature, deaerate and fill in a container.

Example 11 Powder Foundation (1) Liquid paraffin 5.0 (% by weight) (2) Octyldodecyl myristate 2.5 (3) Vaseline 2.5 (4) Methyl parahydroxybenzoate 0.1 ( 5) Fragrance 0.1 (6) Highly active thioredoxin yeast extract 4 0.1 (7) Nylon powder 10.0 (8) Mica 20.0 (9) Talc 43.8 (10) Titanium oxide 9.9 ( 11) Bengala 3.0 (12) Yellow iron oxide 2.5 (13) Black iron oxide 0.5 Production method: The powder components of (6) to (13) are mixed and pulverized through a pulverizer. This is transferred to a high-speed blender, and (1) to (5) are mixed and added, and mixed uniformly. This is processed in a crusher,
After passing through a sieve to adjust the particle size, the mixture is filled in a metal plate and compression molded.

Example 12 Hand Cream (1) Cetanol 4.00 (% by weight) (2) Vaseline 2.00 (3) Liquid paraffin 10.00 (4) Glyceryl monostearate 1.50 (5) Polyoxyethylene (60E.O.) glyceryl 2.50 Isostearate (6) Tocopherol acetate 0.25 (7) Glycerin 20.00 (8) Methyl parahydroxybenzoate 0.10 (9) Thioredoxin highly active yeast extract 1 0.15 (10) Thioredoxin highly active yeast extract 3 0.15 (11) Dipotassium glycyrrhizinate 0.10 (12) Purified water 59.25 Production method: Mix oil phase components (1) to (6), Dissolve to 75 ° C. On the other hand, the aqueous phase components (7) to (12) are mixed and dissolved by heating to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified by a homomixer.

Of the above examples of the external preparation for skin according to the present invention, Examples 1 to 7 and Example 12 were evaluated for the effect of suppressing the formation of wrinkles on the skin by medium wavelength ultraviolet (UVB). At that time, in each Example, the thioredoxin highly active yeast extracts 1 to 4 which were respectively mixed were used as wild-type Sa strains showing normal thioredoxin activity.
Comparative examples 1 to 7 and 12 were used as substitutes for the yeast extract similarly prepared using ccharomyces cerevisiae Meyer, and the evaluation was performed simultaneously. For evaluation, five hairless mice were used as one group, and the examples and the comparative examples were applied to the back once a day for each group, and 100 mJ / cm ^2.
UVB was irradiated three times a week for 20 weeks, and the formation of wrinkles on the skin of the hairless mouse was observed and scored according to the criteria shown in Table 2 to evaluate. At this time, a group to which only purified water was applied was used as a control. The results were calculated as the average of each group, and are shown in Table 3 in relation to the number of UVB irradiation days.

[0037]

[Table 2]

[0038]

[Table 3]

As is apparent from Table 3, in the control, the depth of wrinkles formed on the skin reached a medium level when the number of UVB irradiation days exceeded 10 weeks, and deep wrinkles formed after 20 weeks. Was recognized. On the other hand, in each of the groups to which the examples of the present invention were applied, the formation of minute or slight wrinkles was observed after 20 weeks, and the formation of wrinkles was remarkably suppressed. In contrast, in Comparative Examples 1 and 3 containing only the yeast extract of the wild strain, no clear wrinkle formation-inhibiting effect was observed in the application group, and Comparative Examples 2 and 12 further containing tocopherol acetate and the like. In Comparative Example 6 coated group containing yeast extract in the form of liposome,
Only a slight effect of suppressing wrinkle formation was observed. In addition, the application group of Comparative Example 4, Comparative Example 5 and Comparative Example 7 containing an ultraviolet absorber, a horse chestnut extract and lipoic acid showed a good wrinkle formation-inhibiting effect. The degree was smaller than that of.

Subsequently, use tests were conducted on Examples 1 to 12 of the present invention to evaluate the effects of improving skin aging and rough skin. At that time, Examples 8 to 11 were used.
Also in Comparative Examples 8 to 11, each thioredoxin highly active yeast extract was replaced with a yeast extract similarly prepared by a wild-type Saccharomyces cerevisiae Meyer.

The effect of ameliorating the aging of the skin was evaluated by the group consisting of 20 female panelists in their 40s and 60s in which fine wrinkles and decreased skin elasticity were observed. Evaluation was performed by using the product twice a day in a blind for two consecutive months. The degree of fine wrinkles was evaluated by visual observation and photography, and skin elasticity was measured with a cute meter. The conditions before and after the start of the use test were compared, and the results were "improved", "slightly improved", and "changed". None ". The results are shown in Table 4 by the number of panelists who obtained each evaluation.

The improvement effect of the rough skin symptoms was obtained by grouping 20 female panelists in their 20s and 60s who exhibited remarkable rough skin symptoms in each group with the examples and comparative examples being blinded for 2 days a day. The evaluation was carried out for two consecutive months. The skin condition before and after the start of the use test was evaluated and scored according to the evaluation criteria shown in Table 5, and the average value of 20 persons was calculated and shown in Table 6.

[0043]

[Table 4]

[0044]

[Table 5]

[0045]

[Table 6]

As is evident from Table 4, in the group to which the examples of the present invention were applied, a clear improvement was observed in the panelists with fine wrinkles of 30% or more and skin elasticity of 25% or more. There were no panelists who disapproved. As shown in Table 6, favorable improvement was also observed in the skin roughening symptoms in all the groups applied with the examples, and the skin condition was improved to almost a good condition.

On the other hand, as is clear from Table 4, the degree of improvement in fine wrinkles and skin elasticity was smaller in the comparative example-applied group than in the corresponding example-applied group. Further, as is apparent from Table 6, in the application groups of Comparative Examples 1 to 3, Comparative Example 6, Comparative Example 8, Comparative Example 11, and Comparative Example 12, the improvement of the skin roughness was insufficient, and Comparative Examples 4 and Comparative Examples 5, although a good skin roughness improving effect was observed in the application groups of Comparative Example 7, Comparative Example 9, and Comparative Example 10, the degree was smaller than in the corresponding Example application groups.

[0048]

As described in detail above, according to the present invention, a skin external preparation effective for activating physiological functions of the skin and for preventing and improving skin roughness and skin aging can be obtained.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 7/00 A61K 7/00 W X 7/02 7/02 Z 7/035 7/035 7/42 7/42 7 / 48 7/48 35/72 35/72 A61P 17/00 A61P 17/00 // A61K 38/00 A61K 37/02 (72) Inventor Yuri Okano 6-13 Minatomachi, Minatojima, Chuo-ku, Kobe City, Hyogo Prefecture Noevir Kobe Research Institute Co., Ltd. (72) Inventor Masumi Takei 112-1 Nogami, Okada-cho, Yoka City, Shiga Prefecture Inside Noevir Shiga Central Research Laboratory Co., Ltd. 1 One Maru Falcos Co., Ltd. (72) Katsuko Minoura Inventor, 318 Asagi, Mamasa-cho, Motosu-gun, Gifu Prefecture F-term (Ref.) 4C083 AA071 AA072 AA082 AA112 AB032 AB232 AB242 AB432 AB442 AC012 AC022 AC072 AC102 AC122 AC182 AC212 AC342 AC352 AC422 AC432 AC442 AC472 AC482 AC582 AC782 AC862 AD072 AD092 AD112 AD152 AD162 AD172 AD282 AD53 2 AD662 CC04 CC05 CC12 CC14 CC19 DD22 DD27 DD31 DD32 DD33 DD41 DD45 EE12 EE16 EE17 4C084 AA02 BA44 CA05 MA63 NA14 ZA892 ZC012 4C087 AA01 AA02 BC11 CA16 MA63 NA14 ZA89 ZC01

Claims (2)

[Claims] An external preparation for skin characterized by containing one or more selected from yeast extracts having high thioredoxin activity. 2. The yeast having a high thioredoxin activity is Sacc
The external preparation for skin according to claim 1, which is a thioredoxin-rich strain isolated from haromyces cerevisiae Meyer.