JPH10236941A - Skin lotion - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin lotion that normalizes redox regulatory system in an organism, activates the intracellular peroxide deletion system and can effectively prevent skin disturbance and aging caused by oxidation stress due to ultraviolet rays and metabolism in vivo and can improve them. SOLUTION: This skin lotion contains extract from flesh of pearl shells such as Pinctada martensii, Pinctada margaritifera, Pinctada maxima, Hyriopsis schlegeli. As an extracting solvent, may be used a polar organic solvent, in addition to water, as a lower alcohol, for example, methanol, ethanol, propanol, isopropanol, as a polyhydric alcohol, for example, 1,3-butylene glycol, propylene glycol, dipropylene glycol, glycerol and the like, as an ether, for example, ethyl ether or propyl ether, as an ester, for example, ethyl acetate or butyl acetate, acetone or ethyl methyl ketone, or their mixture, physiological salt solution or phosphate buffer solution. The content of the extract is about 0.01-5.0wt.% in the skin preparation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention activates an intracellular peroxide scavenging system and promotes skin aging symptoms such as skin inflammation, wrinkles and spots caused by oxidative stress caused by ultraviolet rays and metabolism in the body. And a skin external preparation capable of preventing or improving such disorders. More specifically, the present invention relates to an external preparation for skin comprising a pearl shellfish meat extract.

[0002]

2. Description of the Related Art Reactive oxygen species generated by extraneous stimuli such as ultraviolet rays, radiation, heavy metals, and metabolism in a living body cause various reactions in the living body of an animal. It is known to be involved. In particular, it has been reported that hydrogen peroxide among reactive oxygen species is accumulated in cells due to ultraviolet rays or the like. It is said that the Fenton reaction is catalyzed from the hydrogen peroxide by iron ions or copper ions present in trace amounts in skin tissue, and a hydroxyl radical having the highest tissue damage is generated.

In recent years, substances having a scavenging effect on active oxygen species such as hydroxy radicals have been screened, and plant-derived components such as vitamin E group compounds and tea tannin are incorporated into an external preparation for skin. Attempts have been made. In addition, chelating agents have been added to inhibit the generation of hydroxy radicals. Furthermore, a substance that eliminates hydrogen peroxide, which is a precursor for the production of hydroxyl radicals, has been searched, and a button extract has been known (Japanese Patent Application No. 7-191060).

[0004] However, active oxygen species such as hydrogen peroxide and hydroxy radical are involved in a chain oxidation reaction,
Even if these are individually erased, they are not enough to prevent a series of peroxidation reactions in a living body. On the other hand, a redox control mechanism involving thioredoxin, glutathione, or the like exists in the living body, and functions to keep the oxidation-reduction state in the living body constant.

[0005]

Therefore, in the present invention, the normalization of the redox control system in the living body and the activation of the intracellular peroxide scavenging system in the living body allow the oxidation caused by ultraviolet rays and metabolism in the living body to occur. An object of the present invention is to provide a skin external preparation that can effectively prevent and improve skin injuries and aging caused by stress.

[0006]

Means for Solving the Problems To solve the above problems, the present inventor has determined that a substance having an activity of activating an intracellular peroxide scavenging system in a living body is used as an index of a protective action against cell injury caused by hydrogen peroxide. Screening was performed. As a result, a high intracellular peroxide scavenging activating effect was found in the pearl clam shell meat extract, and the present invention was completed by including this in a skin external preparation.

That is, the present invention comprises a skin external preparation base containing an extract obtained from pearl shellfish meat.

[0008]

The protective effect of the pearl mussel shell meat extract contained as an active ingredient in the present invention against cell damage by hydrogen peroxide is shown below.

[0009] Human-derived fibroblasts are seeded in a 96-well microplate at 2.0 × 10 ⁴ cells / well, and after 24 hours, an aqueous extract of pearl mussel is added to each of 0.1 and 0.125. , 0.25, 0.5 and 1 mg / ml
H containing 1 mM of 1 mM hydrogen peroxide
Anks solution was brought into contact with the cells and cultured at 37 ° C. for 2 hours. Next, the medium was replaced with a medium containing 20 μg / ml of neutral red and cultured at 37 ° C. for 2 hours. Neutral red incorporated in the cells was extracted and the absorbance at 550 nm (A ₁ ) was measured. On the other hand, the absorbance (A ₀ ) at 550 nm when only the water extract of the pearl mussel was added and the same treatment was carried out without adding hydrogen peroxide was also measured. The survival rate of the cells at each concentration of the water extract of the pearl mussel was determined by the formula (1). The cell viability was measured six times for each addition concentration, and the average value is shown in Table 1.

(Equation 1)

[0010]

[Table 1] As is evident from Table 1, the water extract of the pearl mussel was added to 0.1%.
When 125 mg / ml or more was added, a significant increase in cell viability was observed, indicating that the compound had a favorable intracellular peroxide scavenging system activating action.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The pearl shellfish meat extract contained in an external preparation for skin as an active ingredient in the present invention is used for collecting natural pearls and for cultivating cultured pearls such as pearl oysters, black mussels, white mussels, and mussels. Than meat, water, methanol, ethanol, propanol,
Lower alcohols such as isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether; esters such as ethyl acetate and butyl acetate; acetone and ethyl It is obtained by extraction with a polar organic solvent such as methyl ketone or a mixture thereof. As the extraction solvent, other than the above, physiological saline, phosphate buffer and the like may be used.

For the extraction from the shell meat, the shell meat may be used as it is, but it is preferable to perform the extraction after processing such as shredding, homogenizing, drying, and pulverizing from the viewpoint of extraction efficiency. The extraction temperature is suitably about 0 ° C to 50 ° C.

The external preparation for skin according to the present invention further comprises other active oxygen scavengers, anti-inflammatory agents, whitening agents, skin cell activators,
In addition to the bactericide, general external preparations such as oils, surfactants, humectants, ultraviolet absorbers, powders, fragrances, preservatives, and raw materials for cosmetics can be contained.

The external preparation for skin according to the present invention can be provided in the form of lotions, emulsions, gels, creams, ointments and the like, and skin cosmetics such as lotions, emulsions, creams, packs and the like. Also provided as makeup base lotion, makeup base cream, liquid or creamy or ointment type foundation, makeup cosmetics such as eye color, cheek color, body cosmetics such as hand cream, leg cream, body lotion, etc. be able to. The content of the pearl shellfish extract in the external preparation for skin is suitably about 0.01 to 5.0% by weight in consideration of bioavailability in the external preparation base.

[0015]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples. First, a production example of a pearl clam shell meat extract to be contained as an active ingredient in the present invention will be described.

[Preparation Example 1] Pearl oyster shellfish extract 1 1 kg of pearl oyster shell meat was homogenized in 2.0 l of purified water at 10 ° C, extracted with stirring for 24 hours, and filtered to collect a filtrate.

[Preparation Example 2] Pearl oyster shell meat extract 2 1 kg of pearl oyster shell meat was extracted at 10 ° C. with 2.0 L of physiological saline.
The mixture was homogenized in water, extracted with stirring for 24 hours, and then filtered to collect the filtrate.

[Preparation Example 3] Pearl oyster shellfish extract 3 1 kg of pearl oyster shell meat was homogenized in 2.0 l of 0.05M phosphate buffer (pH 7.0) at 15 ° C.
After stirring and extracting for a time, the filtrate was collected by filtration.

[Preparation Example 4] Black mussel shell meat extract 1 kg of black mussel shell meat was cut into small pieces, immersed in 2.0 liters of a 50% by volume aqueous ethanol solution at 20 ° C., allowed to stand for 5 days, and the supernatant was recovered. .

[Production Example 5] White mussels shellfish extract 1 kg of white mussels were cut into small pieces, immersed in 2.0 l of a 50% by volume aqueous 1,3-butylene glycol solution at 20 ° C., and allowed to stand for 5 days After that, the supernatant was recovered.

[Preparation Example 6] Extract of mussel shellfish extract 1 kg of mussel shellfish was homogenized in 1.5 liter of a mixed solvent of glycerin, dipropylene glycol and isopropanol (volume ratio = 2: 2: 1) at 15 ° C., and 48
After extraction with stirring for a period of time, the mixture was filtered to recover the filtrate.

Next, the formulation of the external preparation for skin according to the present invention will be described. In the following examples, the pearl mussel extract of the above-mentioned production example was used.

[Example 1] Lotion for skin (1) Ethanol 10.0 (wt%) (2) Hydroxyethylcellulose 1.0 (3) Pearl oyster shell meat extract 10.5 (4) Methyl paraoxybenzoate 0.1 (5) Purified water 88.4 Production method: (1) to (5) are mixed and made uniform.

Example 2 Emulsion for skin (1) Stearic acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Vaseline 3.0 (4) Liquid paraffin 7.0 (5) Polyoxy Ethylene (10E.O.) monooleic acid 1.5 ester (6) Tocopherol acetate 5.0 (7) Glycerin 5.0 (8) Methyl parahydroxybenzoate 0.1 (9) Triethanolamine 1.0 (10 ) Purified water 74.7 (11) Pearl oyster shell meat extract 21.0 Production method: The oil phase components of (1) to (6) are mixed, heated and uniformly dissolved, and kept at 70 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and heated to be uniform, and the temperature is set to 70 ° C. The oil phase component is gradually added to the aqueous phase component while stirring to emulsify, and after cooling, (11) is added and mixed at 40 ° C.

Example 3 Skin Gel (1) Dipropylene glycol 10.0 (% by weight) (2) Carboxyvinyl polymer 0.5 (3) Potassium hydroxide 0.1 (4) Methyl paraoxybenzoate 0.1 (5) Purified water 87.8 (6) Pearl oyster shell meat extract 3 1.5 Production method: After dissolving (2) uniformly in (5), dissolve (4) in (1) Add, then add (3) to thicken and add (6).

Example 4 Skin Cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20E.O.) sorbitan 5.0 Monolaurate (8) Propylene glycol 5.0 (9) Paraoxybenzoic acid Methyl 0.1 (10) Purified water 35.4 (11) Pearl oyster shell meat extract 1 1.0 (12) Black mussel shell meat extract 1.0 Manufacturing method: Mix oil phase components of (1) to (7) Dissolve and heat to 75 ° C. On the other hand, the aqueous phase components (8) to (10) are mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified by a homomixer, and after cooling, (11) and (12) are added and mixed at 40 ° C.

Example 5 Oil-in-water emulsion ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 35.4 (7) Pearl oyster shell meat extract 2 0.5 (8) White pearl mussel shell meat extract 0.5 (9) Ikecho mussel shell meat extract 0.5 Production method: Mix and dissolve the oil phase components (1) to (4)
Heat to 75 ° C. On the other hand, dissolve (5) in (6), heat to 75 ° C, add the oil phase component to this, emulsify, and after cooling, add (7) to (9) at 40 ° C and mix. .

Example 6 Lotion (1) Ethanol 10.00 (% by weight) (2) 1,3-butylene glycol 5.00 (3) Black mussel shellfish extract 0.01 (4) White mussel Meat extract 0.01 (5) Fragrance 0.10 (6) Purified water 84.88 Production method: (1) to (5) are sequentially added to (6) and uniformly mixed and dissolved.

[Example 7] Emollient cream (water-in-oil type) (1) Liquid paraffin 30.00 (% by weight) (2) Microcrystalline wax 2.00 (3) Vaseline 5.00 (4) Diglyceryl geo Leic acid ester 5.00 (5) Sodium L-glutamate 1.60 (6) L-serine 0.40 (7) Propylene glycol 3.00 (8) Methyl parahydroxybenzoate 0.10 (9) Purified water 52. 75 (10) Fragrance 0.10 (11) White mussel shellfish extract 0.05 Manufacturing method: (5) and (6) are dissolved in a part of (9) to 50 ° C and 5 ° C.
Add slowly to (4) heated to 0 ° C. with stirring. This was previously mixed and uniformly dispersed in (1) to (3), which were heated and dissolved at 70 ° C., and (7) and (8) were dissolved in the remainder of (9) and heated to 70 ° C. Add while stirring and emulsify with a homomixer. After cooling, (10) and (11) are added and mixed at 40 ° C.

Example 8 Makeup Base Cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Type glyceryl monostearate 2.00 (5) Propylene glycol 10.00 (6) Potassium hydroxide 0.30 (7) Purified water 69.56 (8) Titanium oxide 1.00 (9) Bengala 0.10 ( 10) Yellow iron oxide 0.40 (11) Fragrance 0.10 (12) Pearl oyster shell meat extract 1 0.02 (13) Black mussel shell meat extract 0.02 Production method: Oil phase of (1) to (4) The ingredients are mixed and heated to 75 ° C. to homogeneity. On the other hand, mix the aqueous phase components (5) to (7)
The mixture is heated and dissolved to make the mixture uniform, and the pigments (8) to (10) are added to the mixture and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and (11) to (13) are added and mixed at 40 ° C.

Example 9 Emulsion Foundation (1) Stearic acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitate 9.00 (6) 1,3-butylene glycol 6.00 (7) Potassium hydroxide 0.10 (8) Methyl parahydroxybenzoate 0.10 (9) Purified water 53.52 ( 10) Titanium oxide 9.00 (11) Talc 7.40 (12) Bengala 0.50 (13) Yellow iron oxide 1.10 (14) Black iron oxide 0.10 (15) Fragrance 0.15 (16) Shellfish extract 0.03 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C to make uniform. On the other hand, the aqueous phase components of (6) to (9) were mixed and
The mixture is heated and dissolved to make the mixture uniform, and the pigments (10) to (14) are added to the mixture and uniformly dispersed by a homomixer. The oil phase component is added to the water phase component, and the mixture is uniformly emulsified by a homomixer, then cooled, and (15) and (16) are added and mixed at 40 ° C.

Example 10 Hand cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60E.O.) glyceryl 2.5 isostearate (6) Tocopherol acetate 0.5 (7) Glycerin 20.0 (8) Methyl paraoxybenzoate 0.1 (9) Purified water 59.0 ( 10) Pearl oyster shell meat extract 2 0.2 (11) White pearl oyster shell meat extract 0.2 Production method: Mix and dissolve the oil phase components of (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (9)
Heat to 5 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified and cooled with a homomixer, and (10) and (11) are added and mixed at 40 ° C.

In Examples 1 to 5 among the above Examples of the present invention, the effect of preventing wrinkles caused by ultraviolet rays was evaluated. In addition, what replaced the pearl mussel shell meat extract compounded in each Example with the extraction solvent used for preparation of each extract was made into the comparative example. The wrinkle-preventing effect was obtained by applying five hairless mice to one group, applying Examples and Comparative Examples to the back once a day for each group, and applying 1 J / cm ² / week long-wave ultraviolet light (UVA) for 50 weeks. Irradiation was performed to observe the occurrence of wrinkles in hairless mice, and scored according to the criteria shown in Table 2. At this time, a group to which only purified water was applied was used as a control. The results were calculated as the average value of each group, and are shown in Table 3 in relation to the number of UVA irradiation days.

[Table 2]

[0034]

[Table 3] As shown in Table 3, in the control group, when the number of days of UVA irradiation exceeded 40 weeks, the depth of wrinkles formed reached a moderate level, and after 50 weeks, deep wrinkles were observed. Was. On the other hand, the wrinkles were significantly suppressed in each of the application groups of the examples of the present invention, and the occurrence of minor wrinkles was observed in the application group of Example 1 after 50 weeks.
-Only the generation of fine wrinkles was observed in the application group of Example 5. On the other hand, in the group applied with the comparative example, the occurrence of wrinkles was slightly reduced in the group coated with the comparative example 2 containing tocopherol acetate, but no significant wrinkles were prevented or reduced.

Next, a practical use test for six months was performed on Examples 1 to 10 of the present invention. As panelists, women in their 40s and 60s who often work outdoors and have skin symptoms such as wrinkles and decreased skin elasticity were used, and the number of panelists was 20. In this use test,
As a comparative example, the pearl mussel shell meat extract compounded in each example was replaced with the extraction solvent used for the preparation of each shell meat extract in the same manner as described above. Use test is from April to 1 with a lot of ultraviolet rays.
Over a period of 0 months, each group was blindly using each of the examples and comparative examples. Before the start of the use test and after the end of the use test, the condition of the skin was observed, and the wrinkle and skin elasticity were evaluated in three stages of "improvement", "slight improvement", and "no change". The degree of wrinkles was evaluated by taking a photograph and a replica, and the skin elasticity was measured and evaluated by a cute meter. Result is,
Table 4 shows the number of panelists who obtained each evaluation.

[0036]

[Table 4] As shown in Table 4, the improvement of wrinkles was observed in all the cases except that one paneler who did not show any improvement was observed in the group using Example 6 of the present invention. In particular, in the groups using Examples 1 to 5 and Example 10, a clear improvement was recognized in 50% or more of the panelists. Regarding the state of improvement of skin elasticity, improvement tendency was observed in all the groups using the examples, and clear improvement was recognized by 65% or more of the panelists in the groups using the examples 1 to 5 and the example 10. On the other hand, in the group using the comparative example, there were no panelists who observed a clear improvement in both wrinkles and skin elasticity, and no improvement was observed in 70% or more of wrinkles and 60% or more of skin elasticities. .

In Examples 1 to 10 of the present invention, no change in state such as precipitation, separation, agglomeration, discoloration, and odor of the contained components was observed at all during the above use test period. In addition, in each group used in Examples, there was no paneler showing a skin irritating reaction or a skin sensitizing reaction.

[0038]

As described in detail above, according to the present invention, the intracellular peroxide scavenging system is activated, and skin inflammation due to oxidative stress caused by ultraviolet rays and metabolism in a living body, wrinkles and spots are generated. A stable and safe external preparation for skin which can prevent or improve disorders such as development of skin aging symptoms could be obtained.

Claims (3)

[Claims] 1. An external preparation for skin comprising a pearl shellfish meat extract. 2. The pearl mussel extract has a content of 0.01%.
The external preparation for skin according to claim 1, wherein the amount is from 5.0 to 5.0% by weight. 3. The external preparation for skin according to claim 1, wherein the external preparation for skin is a cosmetic.