JPH07102252A - Antioxidant - Google Patents

Abstract

PURPOSE:To obtain a substance which is useful as the material of cosmetics, is safe for the human skin, has an antioxidant action, and is effective in preventing wrinkles on the human skin by hydrolyzing a mucus of a shellfish, esp. a pearl oyster. CONSTITUTION:A mucus is obtd. by removing the shell of a shellfish and pressing or centrifuging the residue. The mucus is desalted and concentrated by a treatment such as precipitation by heating or with ethanol and then is hydrolyzed with an acid, an alkali, or a protease (e.g. trypsin or papain). Using the enzyme is suitable since its specificity enables the production of a desired peptide while reducing the amts. of by-products. Desalting the resulting hydrolyzate with 40-80% ethanol is pref. since the coloration of the hydrolyzate is reduced-A cultured pearl oyster is pref. as the shellfish from the viewpoint of waste treatment.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antioxidant obtained from a substance which has been used for many years in foods, pharmaceuticals, etc. for other purposes and whose safety is guaranteed.

[0002]

2. Description of the Related Art Various substances are known as substances which can be used as a raw material for cosmetics and have an antioxidant action, but synthetic products have no guarantee of safety when applied to human skin for a long period of time. , Its use is being limited. On the other hand, many natural products have weak antioxidant effects. However, it is a natural product from the viewpoint of safety for human skin, and is guaranteed in terms of safety by being eaten by humans for many years. In addition, it has a strong antioxidative effect and also has other effects on the skin. Material was desired.

The applicant of the present invention has proposed a method for producing a cosmetic raw material characterized by hydrolyzing the mucus of shellfish in Japanese Patent Laid-Open No. 63-57507. Mucus recovery
The shells of shellfish are dehulled (the ones containing pearls are dehulled after taking out the pearls), and they are subjected to decantation, filtration, centrifugation or the like with or without pressure.

As a shellfish that is a mucus source, pearl oysters (P
ictada martensii), mussel (Myt)
ilus coruscum), mussel (My)
Suitable for use are Uridaceae, Musselidae, Musselidae and the like such as riopsis schlegelii) and crow mussels (Cristaria plicata), but other shellfish can be freely used and there is no problem in terms of raw material supply. Particularly preferred are pearl oysters.

The mucus collected as described above may be subjected to a desalting operation such as hydrolysis or dialysis as it is, but since the treated amount becomes large when a large amount of seawater is contained, it is heated or water-soluble organic. A solvent such as ethanol, methanol, isopropanol, acetone, or an inorganic salt such as ammonium sulfate or sodium chloride is added for precipitation, and the precipitate is collected by centrifugation, filtration or the like to reduce the treatment amount.

However, since the heat treatment may cause only part of precipitation, most of it precipitates when heated to about 120 to 150 ° C. Therefore, the optimum treatment is selected according to the application. When the precipitation treatment is performed, the subsequent treatment is facilitated as described above, the effect as a cosmetic raw material is further enhanced, and the preservability is also enhanced. In order to achieve these objects, in addition to the precipitation treatment, freeze-drying or low-pressure low-temperature concentration treatment may be performed by a conventional method. These processes are
They can be used alone or in combination.

[0007] Shellfish mucus is hydrolyzed with an acid, an alkali, or an enzyme with or without a precipitation treatment to lower the molecular weight of the mucus component. For the hydrolysis, an acid such as hydrochloric acid, sulfuric acid, acetic acid or lactic acid can be used under the necessary conditions of concentration and temperature. As alkali, NaO
H, NH ₄ OH, etc. can be used.

When hydrolyzing with an enzyme, a proteolytic enzyme such as trypsin, papain, pronase, an enzyme of animal and plant origin, or a microorganism origin can be appropriately used. If an enzyme is used, the required molecular weight and the desired peptide can be obtained by utilizing the specificity of the enzyme, and there is no decomposition of tryptophan and the like, and there are few by-products due to the decomposition. In this way, shellfish mucus is decomposed to a required molecular weight by using acid, alkali and enzyme to desalt. Alternatively, it is desalted and then decomposed.

Desalting is not always necessary, but in cosmetics, particularly emulsions, emulsion destruction may occur, so desalting is preferred depending on the application. Desalting is performed, for example, as follows: a method of repeatedly washing with water or an aqueous ethanol solution of about 40 to 80%; a method of dialysis, gel filtration, ultrafiltration, salting out; cation exchange resin, snake A method using a gage ion exchange resin; a method using a chelate exchange resin as a Cu ⁺⁺ type; a method by chromatography. These desalting treatments may be used alone or in combination.

The desalting method uses water, or
Of course, when 0-80% ethanol is used, it can only be used after the sample has been precipitated. Further, when water is used as the desalting method, a part of the sample may be washed away, so it may be used when the precipitation method is a method of heating and precipitating.

With 40 to 80% ethanol, the product is less colored and good results are obtained. The method in which the chelate exchange resin is used as the Cu ⁺⁺ type by the desalting method has an advantage that the amount of the resin is smaller than that of other ion exchange resins when the salt content is large.

The desalting treatment may be performed after the hydrolysis treatment as described above, or before the hydrolysis treatment. If necessary, it may be performed before and after hydrolysis. However, when it is carried out after hydrolysis, it is preferable to carry out a treatment such as an ion exchange resin and chromatography in order to avoid runoff of the active ingredient since the sample has a low molecular weight due to hydrolysis.

In this Japanese Patent Laid-Open No. 63-57507,
Although the method for producing a cosmetic raw material for hydrolyzing the mucus of shellfish is described, the effect of the raw material is only a description that there is a skin conditioning effect.

[0014]

The object of the present invention is to find a substance which is a natural product and is known to be safe for the human body, and yet has a strong antioxidant action. If possible, it is to provide an antioxidant that exerts an effect other than the antioxidant effect. Another object of the present invention is to provide a novel use of the one obtained from shellfish mucus.

[0015]

Means for Solving the Problems The present inventors have completed the present invention by discovering that this substance has an antioxidant action while researching new effects of the substance obtained from shellfish mucus.

That is, the present invention is as follows. (1) An antioxidant containing a substance obtained by hydrolyzing the mucus of shellfish.

(2) The antioxidant according to the above (1), which comprises a substance obtained by a step including a desalting treatment before and / or after the hydrolysis step.

As for the method of utilizing the mucus of shellfish, any kind of mucus of shellfish may be used, but the pearl oyster used for pearl cultivation has problems such as marine pollution after pearl cultivation and is not edible except for scallops. The inventors consider that pearl oysters should be used.

[0019] The waste pearl oysters after collecting the pearls are shelled,
This is decanted or centrifuged to obtain mucus. Ethanol is added to this mucus, left to stand and centrifuged. Repeat this several times. To this, add purified water and stir, then add proteolytic enzyme and stir for about 50 minutes.
Leave at ℃ for about 6 hours. Then, at about 100 ℃, 30
After heating for about a minute, filtration (Toyo filter paper No5C) is performed. This is freeze-dried to obtain a hydrolyzate of shell mucus.

This substance may be used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various additives, humectants such as glycerin and 1,3 butylene glycol, and various agents. It can be added to adjust various dosage forms of cosmetics. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

[0021]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples. (Production example) Akoya oysters (Picta ma
rtensi, shellfish after pearl collection) was used as a raw material.
10.0 kg of the above pearl oysters were dehulled and decanted or centrifuged to obtain 270 g of mucus.

An equal amount of ethanol was added to 100 g of this mucus, and the mixture was allowed to stand at 5 ° C. and then centrifuged. 20 ml of purified water was added to the residue and stirred, 20 ml of ethanol was added and stirred, and then the mixture was centrifuged. This was repeated 8 times. To this, add 200 ml of purified water and stir, then actinase E
0.5 g of (trade name) was added, and the mixture was left at 50 ° C. for 6 hours with stirring. Then, after heating at 100 ° C. for 30 minutes, it was filtered (Toyo filter paper No. 5C). This was freeze dried.

(Example 1) Lotion (% by weight) Olive oil 0.5 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 2.0 Ethanol 10 5.0 Production Example 5.0% Aqueous Solution 5.0 Purified Water 80.5

Example 2 Cream (wt%) A Squalane 20.0 Olive Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerine Monostearate 1.0 Sorbitan Monostearate 2.0 B Purified water 43.9 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5.0 Production example 5.0% aqueous solution 5.0 1.0% sodium hyaluronate aqueous solution 5.0 methyl paraoxybenzoate 0.1 Weigh each A and B, warm to 70 ° C, gradually mix B with A while stirring After adding, slowly stirring 30
Cooled to ° C.

(Antioxidant test) The following test solutions were prepared in 50 ml test tubes with screw caps. Specimen (lyophilized product of production example) 5 mg 2% ethanol solution of linoleic acid 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml purified water 5 ml This is left in a thermostat bath at 50 ° C in the dark. The following measurements were made before putting this in a constant temperature bath and at intervals of several days. Test solution 0.125 ml, 75% ethanol 12.125 ml, 3
0.125 ml of 0% ammonium thiocyanate was added, and the mixture was stirred and allowed to stand for 3 minutes, then 0.02N ferrous chloride 3.5% HC
0.125 ml of an aqueous solution was added, and the mixture was stirred and allowed to stand for 3 minutes, and then the absorbance was measured at a wavelength of 500 nm. The cell length is 10 mm, and the control cell has the test solution replaced with water. This time, the sample is 1 mg,
It was measured to be 2 mg and 3 mg. The results are shown in Table 1.

[0026]

[Table 1]

(Use test) The faces of 6 women were divided into left and right sides, one of which was used as the lotion and cream of Example 1 and the other of which was used as the lotion and cream of Comparative Example 1 one or more times daily. After that, I did a questionnaire. In addition,
Comparative Examples 1 and 2 are prepared by replacing the hydrolyzed liquid of shellfish mucus in Example 1 and 2 with water. (Comparative Examples 1 and 2) The criteria for judgment are as follows, and the results of the questionnaire for these scores are summed up in Table 2 below.

[0028]

[Table 2]

[0029]

EFFECTS OF THE INVENTION It has been revealed that when a hydrolyzate of shellfish mucus is incorporated into cosmetics, quasi drugs, foods, etc., it exhibits an excellent antioxidant effect. When added to cosmetics, it has a great effect of preventing wrinkles on the skin. Since the scallops are edible, long-term safety to the human body is guaranteed.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI technical display location A61K 35/56 7431-4C 47/46 K

Claims (2)

[Claims] 1. An antioxidant containing a substance obtained by hydrolyzing mucus of shellfish. 2. Before and / or after the hydrolysis step,
The antioxidant according to claim 1, comprising a substance obtained by a step including a desalting treatment.