JP2003300821A - Skin care preparation for external use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use that is capable of effectively utilizing pearls and a pearl layer. <P>SOLUTION: This skin care preparation for external use comprises pearls and/or shells containing the pearl layer and at least one kind of substance selected from the group consisting of conchiolin, amber conchiolin, collagen, elastin, acidic mucopolysaccharide, shell acidic polysaccharide and glycogen. The preparation may additionally contain at least one selected from glycolic acid, ascorbic acid, ascorbate salts and ascorbic acid derivatives. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

INDUSTRIAL APPLICABILITY The present invention is characterized in that pearls and / or pearl-containing shells and one or more of conchiolin, amber concholine, collagen, elastin, acidic mucopolysaccharide, shellfish acidic polysaccharide, and glycogen are blended. And an external preparation for skin.

[0002]

2. Description of the Related Art Application of pearls or nacreous layers of shells to cosmetics is disclosed in JP-A-61-24669 and JP-A-6-284.
It has been publicly known in Japanese Patent Application No. 016, and has been used for skin in China and Japan since ancient times.

[0003]

DISCLOSURE OF THE INVENTION The present inventors have diligently studied in order to utilize pearls and pearl layers more effectively.

[0004]

[Means for Solving the Problems] Pearls (including the nacreous layers of shells) and conchiolin, amber conchiolin, collagen, elastin, acidic mucopolysaccharides, shellfish acidic polysaccharides, and glycogen that are highly effective and highly safe By combining the above, not only the efficacy of pearls (including the nacreous layer of shells) but also the efficacy of conchiolin, amber concholine, collagen, elastin, acidic mucopolysaccharides, shellfish acidic polysaccharides, glycogen is combined, and as a skin external preparation. A formulation with high efficacy was completed. Furthermore, by blending at least one of glycolic acid, ascorbic acid, ascorbic acid salt, and ascorbic acid derivative, a superior external preparation for skin was obtained.

Shells containing pearls and nacres are pearl oysters,
It may be selected from fresh-water and sea-water products such as shore mussel, black-lipped mussel, and pearl mussel. You may mix only the nacre of the shell containing the nacre, and it is obtained by removing the columnar layer etc. from these shells. As the method, a known method such as Japanese Patent Publication No. 6-43320 may be used.
Actually, the nacre is harder than the ridge layer, so this difference is used to physically and chemically remove it. Further, this is powdered and used. As will be described below, it is more effective to carry out the step of powdering sufficiently when it is not dissolved with glycolic acid, ascorbic acid, ascorbic acid salt, ascorbic acid derivative or the like.

Conchiolin is a kind of hard protein contained in shells and pearls, and is relatively contained in pearl oysters, mussels, mussels and the like. The manufacturing method is the above-mentioned Patent
87730, Patent 1560610, Patent 15877
34, etc.

That is, the shells and the like are crushed to remove the calcium content with dilute hydrochloric acid and the like, and the insoluble matter is collected by a solid-liquid separation method such as centrifugation and filtration decantation, which is then stirred well with purified water. Collect insolubles by centrifugation, filtration, etc. Repeat this if necessary.

Further, a 2 to 10% aqueous hydrochloric acid solution may be added to this conchiolin to hydrolyze it at 50 to 110 ° C. for 5 hours to 5 days to obtain a conchiolin hydrolyzate. This is sometimes simply called conchiolin.

After hydrolyzing with dilute sulfuric acid instead of dilute hydrochloric acid, neutralizing with barium hydroxide, and further neutralizing with alkaline hydroxide solution to pH 5-6, the precipitate is centrifuged. The hydrolyzed liquid removed by separation, filtration and the like is made into a concentrated liquid or a dry powder by using a known concentration method or drying method. When hydrolyzing, the acid concentration should be adjusted so that conchiolin is not completely decomposed into amino acids.
Of course, the temperature and time should be controlled.

[0010] Amber conchiolin is a substance obtained by treating a hydrolyzate of conchiolin with succinic anhydride, and its production method is described in JP-A-7-165526. In this preparation method, succinic anhydride is added while stirring in an aqueous solution of a hydrolyzate of conchiolin and maintaining the pH at 6-9. It is not necessary to succinylate the hydrolysis rate of conchiolin or all of them, so the amount of succinic anhydride should be adjusted according to the purpose.

Collagen is a major protein forming animal skin such as cowhide, pig skin, and fish skin. Collagen is
It is an important connective tissue protein that occupies 70% of the dermis and is involved in the formation of skin firmness and wrinkles, and also has a high moisturizing property and also affects the freshness of the skin. And, it is already widely used as an external preparation for skin. Various sources of collagen can be selected such as cow skin, pig skin, and fish skin. Recently, collagen derived from marine products such as fish skin has been attracting attention due to the problem of infectious diseases. Among them, Japanese Patent Application No. 2001-153.
Collagen derived from shellfish filed in No. 341 is most suitable. As for the manufacturing method, pearl oyster cultivation is finished for pearl oysters,
When removing the pearls, the scallops collect the meat after removing the scallops. Water is added to this and stirred to collect insoluble matter. The method may be a known method. That is, for example, a method of adding water and then stirring and then separating with a centrifuge or a method of leaving the supernatant after leaving it still may be selected. To further remove impurities from this insoluble matter, salt solutions such as sodium chloride, potassium chloride, sodium acetate, sodium citrate, sodium hydrogen phosphate, sodium hydroxide, potassium hydroxide, calcium hydroxide,
A basic solution such as aqueous ammonia or a chelating agent such as ethylenediaminetetraacetate is added and stirred to collect insoluble matter. After further removing impurities with a water-soluble organic solvent such as ethanol, collagen is obtained by acid extraction with acetic acid, citric acid, lactic acid, hydrochloric acid, phosphoric acid or the like. The concentration varies depending on the type of acid and various conditions, but is 0.
It is carried out at a concentration of 01 to 2 molar. It is extracted by adding this solution and stirring for 2 to 48 hours. The temperature is 30 ° C or lower, preferably 2 to 10 ° C. In addition, the enzyme may be used, and then extraction may be performed by a known method such as an acid extraction method.
Further, depending on the application, it may be better to further purify, and it is purified using a hydrophilic organic solvent, a salting-out method or the like. Elastin, together with collagen, is a protein involved in skin elasticity.

Acid mucopolysaccharides include hyaluronic acid, teichoic acid, colominic acid, chondroitin sulphate, dermatan sulphate, heparin, heparan sulphate, keratan sulphate, etc. These are compounded in the form of salts such as sodium salts and potassium salts. May be. Japanese Patent Laid-Open Publication No. 8-26971 discloses a method for producing shellfish acidic polysaccharides, and the necessary substance may be obtained based on this. Briefly, the method is characterized in that after degrading shellfish with a proteolytic enzyme, deproteinization is performed, low molecular weight substances are removed, and then a quaternary ammonium salt is added to precipitate and decompose acidic polysaccharides. Is a method for producing a cosmetic raw material containing acidic polysaccharide as a main component. Dialysis, gel filtration, ultrafiltration, or an organic solvent that precipitates acidic polysaccharides is used to remove low-molecular substances. Further, in order to purify the acidic polysaccharide separated by precipitation with a quaternary ammonium salt, an inorganic salt aqueous solution is added to the precipitate and dissolved, and then reprecipitated with an alcohol aqueous solution, or by repeating this method. You can The method of the present invention will be described in detail. In the first step, shellfish meat from which shells have been removed is decomposed by a protease. In order to facilitate this treatment, it is preferable to heat and denature and then pulverize with a mixer or the like. When pearl oysters are used, the mucus associated with the shell meat is also used as a raw material, and only mucus may be used. At this time, it can be used regardless of taking out pearls as in the case of using shell meat, the raw material can be easily collected, and the crushing step is not necessary. The proteolytic enzyme is not particularly limited, but one that has no substrate specificity and strong decomposing power is preferable. For example, papain, actinase, samoase, denazyme, etc. 1
Decompose using seeds or a mixture of two or more.

In the second step, deproteinization is performed to remove undegraded proteins, nucleic acids and enzymes. The method of deproteinization is not particularly limited, but generally trichloroacetic acid or perchloric acid is used.
It is used so that the concentration becomes 0% or the Sevag method is used. In the third step, low molecular weight substances such as deproteinizing agents, amino acids, peptides and salts are removed. As the removal method, there are a dialysis method, gel filtration, ultrafiltration, and a method of adding an organic solvent in which an acidic polysaccharide is precipitated, and the dialysis method is preferable. In the fourth step, neutral polysaccharides and glycoproteins that are present together with acidic polysaccharides are separated. As a removing means, an aqueous solution of a quaternary ammonium salt is added. Since the acidic polysaccharide binds to the quaternary ammonium salt and precipitates, it is separated by decantation or centrifugation.

As the quaternary ammonium salt, it is sufficient that one of the alkyl groups has 12 or more carbon atoms. Also in the pyridinium type, it is sufficient that the alkyl group bonded to nitrogen has 12 or more carbon atoms. For example, lauryl trimethyl ammonium salt, stearyl trimethyl ammonium salt, cetyl pyridinium chloride, etc. can be illustrated. By the fourth stage, the acidic polysaccharide can be obtained, but since this precipitate contains a quaternary ammonium salt and other salts, further purification is preferable. For purification, an inorganic salt aqueous solution is added to this precipitate to dissolve it, and the complex is dissociated and dissolved, and then alcohol is added to reprecipitate the acidic polysaccharide and the quaternary ammonium salt is separated and removed in the liquid. To do. This precipitate is dissolved in purified water, water is added so that the alcohol concentration becomes 80%, and the mixture is stirred and left to stand to obtain a precipitate by centrifugation.

It is preferable to repeat this step 2-3 times. Any inorganic salt can be used as long as it dissociates and dissolves the complex of the quaternary ammonium salt of acidic polysaccharide. Examples thereof include sodium chloride and potassium chloride. The salt form used here determines the salt form of the final product. For example, if sodium chloride is used, the final product will be the sodium salt. The concentration of the inorganic salt is a concentration required to dissociate the complex, and sodium chloride needs to be 1.5 M or more. There is no particular upper limit, but if the concentration is too high, the desalting operation will take time, waste of reagents will occur, and the limit is about 4M. Furthermore, when it is desired to remove the remaining proteins and dyes, the Lloyd's reagent and cations are added to an aqueous solution obtained by dissolving the precipitate in water to adsorb and remove the dyes and proteins. After removing the adsorbent by centrifugation and filtration, the acidic polysaccharide is freeze-dried.
When the acidic polysaccharide obtained in this manner is incorporated into cosmetics, the effect is that it has high water retention and moisturizing properties like hyaluronic acid, etc., so that it moisturizes the skin and hair and prevents drying, Due to its excellent penetrability and tissue affinity, it showed a unique lubricity effect. In addition, increasing the viscosity of cosmetics itself, improving stability and feeling of use are also important effects.
Is one.

Glycogen has a strong cell activating effect, and among the glycogen, glycogen derived from pearl oyster is highly effective. Glycogen is commercially available from various sources,
Glycogen derived from pearl oysters is disclosed in JP 2001-9784 A.
It is also described in Japanese Patent No. 5 publication. Described below,

The glycogen derived from pearl oysters can be obtained by extracting the flesh and the like of pearl oysters. For example,
A method called hot water method is used to shred pearl oyster shell meat, and then add water if necessary and heat. Then centrifuge. A larger amount of glycogen can be extracted by repeating heating and centrifuging several times, and therefore it is repeated as many times as necessary in consideration of cost and the like. If necessary, remove insolubles by filtration, etc., and after concentrating the filtrate considering the efficiency of the subsequent steps, add trichloroacetic acid to a final concentration of about 5% by weight, and statically cool at low temperature (0-10 ° C). Place. The precipitate generated by this is removed by a method such as centrifugation or filtration. However, since this step is mainly a step of removing proteins, it can be omitted depending on the case. About 3 times as much ethanol is added to the supernatant and the mixture is stirred. Glycogen is obtained by collecting the precipitate generated by this method by centrifugation or filtration. If necessary, add water, and then add about 4 times the amount of water of ethanol (at this time, a small amount of a saturated aqueous solution of calcium chloride can be added to accelerate the precipitation), and the resulting precipitate is centrifuged or filtered. Precipitate again and collect. This can be purified by repeating this for the required number of times. Further, when it is desired to further purify, it is also effective to remove low molecular weight substances by a method such as dialysis.

As another method, dimethyl sulfoxide is added to the flesh of pearl pearl oysters, which is shredded and then centrifuged and the supernatant is suction filtered. The same operation is repeated for the residue, if necessary, and the supernatants are combined. The supernatant is poured into methanol containing about 0.17% by weight of sodium chloride. Collect the precipitate by decantation or centrifugation. This operation is repeated if necessary. Water is added to this precipitate to dissolve it, and the precipitate is precipitated with about 3 times the amount of ethanol. This operation is repeated about 3 times to wash the precipitate. During this period or after the completion of this step, dialysis or the like is carried out as necessary for the step of removing the protein or for further purification.

At least one of glycolic acid, ascorbic acid, ascorbic acid salt, and ascorbic acid derivative is added to this. By compounding these, the effectiveness and the like are further increased. These are methods of dissolving calcium carbonate in pearls or shells containing nacres, and making more effective use of the active ingredients of pearls. Also, glycolic acid, ascorbic acid, ascorbate, ascorbic acid derivatives, etc. A very effective external preparation for skin could be obtained in combination with its own effect. Of course, there is no problem in adding other acids.

Dissolving pearls has the great effect of improving the effectiveness, but when used in transparent or translucent cosmetics, it also has an advantage in appearance, and in a liquid system having a low viscosity, precipitation or the like occurs. It also has the new effect of solving the problem of. The derivative of ascorbic acid is not particularly limited, and examples thereof include ascorbic acid sulfate (salt), ascorbic acid phosphate (salt), and ascorbic acid glucoside. However, according to our experiments, ascorbic acid glucoside was optimal. pearl,
Alternatively, the nacre content of the shell is 0.0001-50%,
Conchiolin, amber conchiolin, collagen, elastin, acidic mucopolysaccharides, shellfish acidic polysaccharides, and glycogen vary greatly depending on the type, but the blending amount is 0.000.
1 to 10% is preferable. In addition, pearls or nacre of shells and conchiolin, amber conchiolin, collagen,
When one or more kinds of elastin, acidic mucopolysaccharides, shellfish acidic polysaccharides, and glycogen are mixed with one or more kinds of glycolic acid, ascorbic acid, ascorbate, ascorbic acid derivative, etc., pearls or nacre of shell and glucole The acid ratio is preferably 0.5: 99.5 to 10:90, and the total amount thereof is preferably 0.5% to 10% in the preparation.
If it is 0.5% or less, the effectiveness is low, and if it is 10% or more, there is almost no merit. The ascorbic acid derivative is preferably 0.2 to 15%, though it varies depending on its type, the proportion of glycolic acid, and the intended use of cosmetics. . The pH of the preparation is preferably 3.0 to 5.5, and the pH is 3.0.
Below, there is a problem of irritation, and at pH 5.5 or above, dissolution may not be completed completely.

The above-defined substances can be mixed in various dosage forms. For example, lotions,
It can be in the form of emulsions, creams, ointments, packs, bath salts.

In order to formulate the dosage forms of these cosmetics,
Natural animal and vegetable oils and fats such as olive oil, mink oil, castor oil, palm oil, beef tallow, evening primrose oil, coconut oil, castor oil, cacao oil, macadamia nut oil and the like; waxes such as jojoba oil,
Beeswax, lanolin, carnauba wax, candelilla wax, etc .; higher alcohols such as lauryl alcohol, stearyl alcohol, cetyl alcohol, oleyl alcohol, etc .; higher fatty acids such as lauric acid, palmitic acid, stearic acid, solid paraffin, squalane,
Vaseline, ceresin, microcrystalline wax, etc .; synthetic ester oils such as butyl stearate, hexyl laurate, diisopropyl adipate, diisopropyl sebacate, octyldodecyl myristate, isopropyl myristate, isopropyl palmitate isopropyl myristate, cetyl isooctanoate ,
Neopentyl glycol dicaprate; Silicone derivatives Silicone oils such as methyl silicone and methyl phenyl silicone

Examples of the surfactant include anionic surfactants such as alkyl sulfates, fatty acid salts, alkyl phosphates, polyoxyethylene alkyl ether phosphates and sulfates; nonionic surfactants such as Glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether, sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyglycerin fatty acid ester, etc .; Double-sided activator such as alkyl betaine, phosphobetaine, phosphatidyl choline, phosphatidyl ethanolamine , Phosphatidylserine, phosphatidylglycerol, phosphatidylinositol and their lyso forms, as well as phosphofatidic acid and its salts

Polyhydric alcohols such as ethylene glycol, diethylene glycol, triethylene glycol,
Further polyethylene glycols, propylene glycol, dipropylene glycol, further polypropylene glycols, 1,3-butylene glycol,
Butylene glycols such as 1,4-butylene glycol, glycerin, diglycerin, and more polyglycerins, sorbitol, mannitol, xylitol,
Sugar alcohols such as maltitol, ethylene oxide (hereinafter abbreviated as EO) of glycerins, propylene oxide (hereinafter abbreviated as PO) adducts, sugar alcohols E
O, PO adducts, saccharides such as galactose, glucose and fructose and their EO and PO adducts, maltose,
Polysaccharides such as lactose and polyhydric alcohols such as EO and PO adducts

As drugs, tocopherol, tocopherol acetate, allantoin, placenta extract, elastin, arbutin, collagen, triclosan, trichlorocarbane, dipotassium glycyrrhizinate, methylparaben, butylparaben

These raw materials are combined to obtain the required formulation. Of course, the use of α-hydroxy acids such as citric acid, malic acid, tartaric acid and lactic acid other than glycolic acid is effective.

Examples will be described below, but the present invention is not limited to these Production Examples and Examples.

Production Example 1 Conchiolin (hydrolyzate) Decalcified pearl oyster-derived conchiolin was used as a dried product for 1 k.
10 g of a liquid obtained by diluting 98% sulfuric acid 30 times with water was added to g, sealed in a glass container, and heated at 110 ° C. for 24 hours. After completion of the decomposition, 90% of the equivalent amount of sulfuric acid used, barium hydroxide, was added little by little with sufficient stirring. Further pH
The mixture was neutralized to 5.8 with a 1% aqueous sodium hydroxide solution. Then, after centrifuging at 2000 G for 10 minutes, it is further filtered through a 0.45 micron membrane filter,
This was freeze dried.

Production Example 2 500 g of amber pearl pearls are gradually added to 1 kg of hydrochloric acid with stirring to decalcify. Further, 300 g of 1N hydrochloric acid was gradually added with stirring to decalcify, and this was filtered to collect insoluble matter. 100 ml of sulfuric acid diluted 30 times with water was added thereto, and the mixture was sealed in a glass container and thermally decomposed at 110 ° C. for 24 hours.
After cooling, 90% of the equivalent amount of sulfuric acid used, barium hydroxide, was added little by little with sufficient stirring. Furthermore, pH 5.8
1% aqueous sodium hydroxide solution was added. Then 200
G, centrifuged for 10 minutes and then filtered through a 0.45 micron membrane filter to obtain a decomposed product. This was freeze dried. Take 5.0 g of this substance and add 100 parts of purified water.
Disperse in 1 ml and stir while stirring 1 g of succinic anhydride to pH 6
~ 9 to 15 while maintaining 1N sodium hydroxide solution
Add 5 times every minute. This was freeze dried.

Production Example 3 100 g of pearl pearl oyster collagen pearl oyster without shells and pearls.
1 liter of a 5 molar sodium chloride solution was added and stirred.
This was centrifuged to collect the insoluble matter. This operation was repeated 3 times. To the obtained insoluble matter, 1 liter of ethanol was added, stirred, and left overnight. This was centrifuged to obtain an insoluble matter. This operation was repeated 3 times. This was added with 1 liter of water, stirred, and then centrifuged to obtain an insoluble matter. To the obtained insoluble matter, 1 liter of a 0.5 mol citric acid aqueous solution was added and stirred for 24 hours. This was centrifuged to remove the insoluble matter, put in a dialysis membrane, and allowed to stand in running water for 2 days. In addition,
All operations were carried out while keeping the temperature at 10 ° C.

Production Example 4 Acid pearl oyster acidic polysaccharide a. 100kg of pearl oysters with shells removed are heated,
After denaturing, it was pulverized with a mixer. b. Add 100g of actinase E to this, 45 ℃
The mixture was left to stand for 24 hours while being stirred. c. 10 kg of trichloroacetic acid was added, and the mixture was stirred, allowed to stand for 1 hour and then centrifuged to remove the precipitate, and deproteinize. Further, dialysis with cellophane tube was carried out for 48 hours to remove low molecular weight substances. d. Then, 1 kg of cetylpyridinium chloride was added, and the mixture was stirred, allowed to stand for 1 hour, and then centrifuged to obtain a precipitate of cetylpyridinium salt of acidic polysaccharide. e. 2M sodium chloride was added to this precipitate to dissolve the precipitate. Ethyl alcohol (3 times) is added thereto, and the mixture is stirred, left standing for 1 hour and then centrifuged to obtain a precipitate. f. A small amount of purified water is added to this precipitate to dissolve the precipitate,
Ethyl alcohol is added to have a concentration of 80%, the mixture is stirred, left for 1 hour and then centrifuged to obtain a precipitate. g. f was repeated 3 times. The yield was 512 g.

Production Example 5 The mussel of pearl oyster was removed on the pearl oyster glycogen wire mesh, and 1500 g of the flesh
Was homogenized with a Waring blender for 2 minutes. To this, 2500 ml of distilled water was added, and the mixture was heated in boiling water for 30 minutes while stirring. The mixture was centrifuged at 350 G for 20 minutes, 1000 ml of distilled water was added to the residue, and the mixture was likewise heated several times until no opalescence was observed. The glycogen extracts were combined and suction filtered using Toyo Filter Paper No. 1
After concentration under reduced pressure to 50 ml, trichloroacetic acid was added so that the final concentration was 5% by volume, and the mixture was allowed to stand at 4 ° C. for 12 hours. 450
After centrifugation at 0 G for 60 minutes to remove proteins, the supernatant was mixed with 3 volumes of ethanol to a final concentration of 75% by volume, and precipitated to obtain crude glycogen. 400 this
The precipitate was dissolved with 1 ml of distilled water and then 1600 ml of ethanol was added to collect the precipitate. This operation was repeated 3 times to wash glycogen. At this time, 1-2 drops of a saturated calcium chloride solution were added in order to completely form a precipitate. The obtained precipitate was dissolved in a small amount of cold distilled water, dialyzed against the same solution, and then centrifuged at 2500 G for 10 minutes,
The supernatant was freeze-dried to obtain pearl oyster-derived glycogen. This pearl oyster-derived glycogen has a purity of 89% by weight.
Was identified by the Anthuron concentrated sulfuric acid method.

[0033] (Example 1) Lotion weight part Olive oil 0.5 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 1.0 1,3 butylene glycol 5.0 Pearl powder (finely ground pearl oyster pearls) 0.1 Purified water 78.4 Production Example 1 1.0

[0034] (Example 2) Lotion part by weight Olive oil 0.5 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 1.0 1,3 butylene glycol 5.0 Pearl powder (finely ground pearl oyster pearls) 0.1 Glycolic acid 2.0 Ascorbic acid glycoside 2.0 Purified water 73.4 Production Example 2 2.0

Example 3 Cream Part by weight A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostea Rate 2.0 B Purified water 51.85 Production Example 3 1.0 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5. 0 1.0% aqueous solution of sodium hyaluronate 2.0 Finely pulverized pearl oyster shell 0.05 Methyl paraoxybenzoate 0.1 A and B are weighed and heated to 70 ° C, and A is stirred to B After adding slowly, slowly stirring 30
Cooled to ° C.

Example 4 Cream Part by weight A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostea Rate 2.0 B Purified water 55.85 Elastin 1% aqueous solution 1.0 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5 0.0 1.0% sodium hyaluronate aqueous solution 2.0 Finely ground pearl oyster shell 0.05 Glycolic acid 5.0 Ascorbic acid glycoside 2.0 Methyl paraoxybenzoate 0.1 A and B were weighed and measured at 70 ° C. Warm up to B, slowly add A to B with stirring, then slowly agitate with 30
Cooled to ° C.

Example 5 The example 1 of the example 1 was replaced with the example 4 of the production, and the other steps were the same as those of the example 1.

Example 6 The example 3 of the example 3 was replaced with the example 5 of the production, and the other steps were the same as those of the example 3.

Example 7 Example 3 was prepared in the same manner as in Example 1 except that sodium chondroitin sulfate was used in Production Example 1 of Example 1.

(Use test) 32 women were divided into 4 groups, the face was divided into right and left, one set with the lotion and cream of the example, and the other set with the lotion and cream of the comparative example. Have it used once or more daily, and after 3 months, feel moisturized, whitened, and radiant on the skin.
The improvement of dullness and the suppleness of the skin were evaluated. In addition, as comparative examples, those prepared by excluding pearl powder from Example 1 and those prepared by excluding finely ground pearl oyster shells from Example 3 were used. The results are shown below. The results were very effective as compared with the comparative example, 3, was effective, was slightly effective, was 1; was not different from the comparative example, was 0, and the comparative example was slightly effective. Tao-1,
Effective was -2, very effective was -3,
The average is shown.

[0041]

[Effects] As described above, it was found that each example is highly effective.

Front page continuation (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 31/375 A61K 31/375 31/718 31/718 31/726 31/726 35/56 35/56 38/17 A61P 17/16 A61P 17/16 A61K 37/12 F Term (reference) 4C083 AA071 AA072 AA082 AA122 AC022 AC072 AC102 AC122 AC301 AC302 AC422 AC432 AC442 AC482 AD241 AD242 AD311 AD332 AD411 AD412 AD431 AD432 AD641 AD642 CC02 CC05 DD31 BA0847AFF01 40847A01 DA40 MA63 NA14 ZA891 ZB221 4C086 AA01 AA02 BA18 EA20 HA27 MA02 MA04 MA63 NA14 ZA89 ZB22 4C087 AA01 AA02 BB16 CA16 MA63 NA14 ZA89 ZB22 4C206 AA01 AA02 DA07 MA02 MA04 NA14 ZA89 ZB22

Claims (2)

[Claims] 1. A skin external preparation containing a shell containing pearls and / or nacre and one or more of conchiolin, amber conquiolin, collagen, elastin, acidic mucopolysaccharide, shellfish acidic polysaccharide and glycogen. 2. The external preparation for skin according to claim 1, containing at least one of glycolic acid, ascorbic acid, ascorbic acid salt and ascorbic acid derivative.