JP3342050B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a novel cosmetic.
More specifically, the present invention relates to a cosmetic composition containing an acylated alkali-treated collagen and proteoglycan as active ingredients and having an excellent effect of improving skin protection.

[0002] In particular, the present invention relates to a cosmetic material which exhibits a remarkable effect when used on the neck.

[0003]

2. Description of the Related Art For the purpose of moisturizing, glycerin,
1,3-butylene glycol, isoprene glycol,
Polyhydric alcohols such as polyethylene glycol and sorbitol, and amino acids such as serine, proline and glutamic acid are blended. Furthermore, mucopolysaccharides such as hyaluronic acid, sodium chondroitin sulfate and dermatan sulfate are also incorporated for the same purpose.

On the other hand, for the purpose of improving skin elongation and elasticity,
Water-soluble collagen, proteins such as elastin,
Polypeptides such as hydrolyzed collagen and hydrolyzed elastin are blended in cosmetics.

[0005] Furthermore, there has been no cosmetics that protect the skin from physical irritation to the rubbed part by inorganic powder mixed with foundation, blusher, tongue and the like, or physical irritation from clothing especially to the neck and the like. there were.

[0006]

SUMMARY OF THE INVENTION Glycerin, 1,3-
Under high humidity, polyhydric alcohols such as butylene glycol work effectively as a skin moisturizer when incorporated into cosmetics. However, under low humidity, these polyhydric alcohols, on the contrary, take up the moisture of the skin into the cosmetic and are not suitable as skin moisturizers.

[0007] When amino acids such as serine, proline, glutamic acid and the like are blended in cosmetics as a humectant, there is a problem that when sugars coexist, browning and smell are caused by the Maillard reaction.

[0008] Mucopolysaccharides such as hyaluronic acid and sodium chondroitin sulfate have very excellent moisturizing effects as moisturizers derived from living organisms. However, skin stretch,
The effect of improving elasticity is far from being superior to proteins and polypeptides.

[0009] Proteins such as water-soluble collagen and elastin, and polypeptides such as hydrolyzed collagen and hydrolyzed elastin are excellent in improving skin elongation and elasticity when incorporated into cosmetics. However, the moisturizing effect is far from superior to mucopolysaccharides.

Therefore, in order to obtain a cosmetic having both a moisturizing effect, skin elongation and elasticity improving effect, attempts have been made to use a mucopolysaccharide such as hyaluronic acid or chondroitin sulfate and a protein such as collagen in combination. . However, when mucopolysaccharides and proteins are used in combination in cosmetics, white precipitates are formed, which has a problem in stability and has not been put to practical use.

[0011]

Means for Solving the Problems In view of such circumstances, the present inventors have made intensive studies and as a result, cosmetics containing acylated alkali-treated collagen and proteoglycan are transparent, have a moisturizing action and It has been found that it prevents moisture loss, has an excellent protective effect on the skin, and also has an effect of improving skin elongation and elasticity. In particular, they have found that these effects are remarkably exhibited when used on the neck, and have completed the present invention.

That is, the present invention is a cosmetic comprising an acylated alkali-treated collagen and proteoglycan. The amount of the acylated alkali-treated collagen used in the present invention is 0.005 as a solid content.
~ 0.5% by weight. The compounding amount of proteoglycan is 0.005 to 2.0% by weight. And the compounding ratio of the acylated alkali-treated collagen and proteoglycan is 100: 1 to 1:50. Preferably 1: 1 to 1:
5

[0013] The acylated alkali-treated collagen is 0.0
When the content is less than 0.05% by weight and the content of proteoglycan is less than 0.005% by weight, the effects of the present invention cannot be sufficiently exhibited, which is not preferable. In addition, acylated alkali-treated collagen is 0.5% by weight or more and proteoglycan is 2.0% by weight.
Although the above blending is possible, no remarkable increase in the effect is observed, stickiness and the like are felt, and the feeling of use deteriorates, which is not economical.

The acylated alkali-treated collagen used in the present invention is obtained by acylating alkali-treated collagen obtained by treating a collagen raw material with alkali.

The alkali-treated collagen is obtained by solubilizing a collagen raw material with an alkali, and has telopeptide removed. This alkali treatment is produced, for example, as follows, but may be produced by another method. The purified insoluble collagen raw material is treated with an alkali, and is solubilized by cutting a telopeptide portion at a molecular end to obtain an alkali-treated collagen.

The acylated alkali-treated collagen is obtained by acylating the alkali-treated collagen with a polycarboxylic acid. This acylation is performed, for example, as follows, but may be performed by another method. Aqueous solution of alkali-treated collagen is adjusted to pH 9-1 with alkali such as caustic soda.
Adjust to 0. Here, a polycarboxylic anhydride dissolved in a solvent such as acetone is gradually added, and an alkali is added and stirred to keep the pH of the alkali-treated collagen solution within the above range. After completion of the reaction, the solution in which the produced acylated alkali-treated collagen is dissolved is adjusted to a pH of about 4.5 with an acid solution such as hydrochloric acid, and the precipitate is collected by centrifugation. The precipitate is washed several times with an acid solution to obtain an acylated alkali-treated collagen.

Although the acylated alkali-treated collagen may be used as it is, in the present invention, an aqueous solution of the acylated alkali-treated collagen is used as the acylated alkali-treated collagen solution. Preservatives and the like may be added as necessary.

The proteoglycan used in the present invention is
Mucopolysaccharide protein complex. The mucopolysaccharide protein complex may contain several or more in the polypeptide chain of the protein,
It is an acidic mucopolysaccharide-protein complex having a molecular structure of about 1,000,000 to 10,000,000 and containing 5 to 50% of protein, with several tens of mucopolysaccharide chains attached. The constituent acidic mucopolysaccharides are mainly hyaluronic acid, chondroitin-4
-Sulfate, chondroitin-6-sulfate, dermatan sulfate,
Chondroitin 6,9-sulfate, chondroitin, heparin, heparitin sulfate, keratosulfate and the like.

In the method for producing a proteoglycan extract used in the present invention, for example, a connective tissue extracted from a cow is sliced under ice-cooling to remove blood vessels, fat, and the like. Next, 0.
Extract with 4M guanidine hydrochloride adjusted to pH 5.8 with 05M sodium acetate. After extraction, the mixture was centrifuged, and the obtained supernatant was dialyzed against 10 volumes of 0.05 M sodium acetate (pH 5.8) for 12 hours at a cold temperature for 12 hours, followed by density gradient ultracentrifugation (25,000 rpm, 20 ° C.). , 45 hours)
To fractionate. Preservatives are added to the fraction obtained by collecting the bottom of the fraction, if necessary. This proteoglycan extract has a protein content of 0.35 to 0.65% by weight. Although it may be used as it is, it may be dried to obtain proteoglycan.

The cosmetic of the present invention can be carried out according to a usual production method, is stable over time, and the lotion is transparent. The cosmetics of the present invention may contain various components generally used in cosmetics, i.e., oils and fats, waxes, hydrocarbons, fatty acids, alcohols, in addition to the above essential components, as long as the effects of the present invention are not impaired. , Synthetic esters, surfactants, humectants, thickeners, inorganic substances, fragrances, drugs, purified water and the like.

[0021]

Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto. The acylated alkali-treated collagen solution and proteoglycan extract prepared in the following Examples were prepared according to the above-described production method.

Example-1 Neck lotion (A) Ethyl alcohol 10.0 Perfume 0.1 Polyoxyethylene (20) hydrogenated castor oil 0.4 Glycerin 5.0 1,3-butylene glycol 4.0 (B ) Acylated alkali-treated collagen solution (0.5% by weight) 20.0 Proteoglycan extract (10% by weight) 5.0 Methyl parahydroxybenzoate 0.1 Citric acid 0.5 Sodium citrate 0.3 Purified water No. 6 after adding (A) to (B). Filter through 5C filter paper. This lotion is transparent. Also, all of the following formulations do not precipitate over time.

Example-2 Neck Cream (A) Squalane 5.0 Behenyl Alcohol 1.5 Cetyl Alcohol 0.5 Polyoxyethylene (20) Sorbitan Monostearate 1.2 Sorbitan Monostearate 0.8 Polyoxyethylene ( 20) Cetyl ether 2.5 Adsorbed and purified lanolin 4.0 Myristyl octyl dodecanol 8.0 Stearic acid 1.2 (B) 1,3-butylene glycol 6.0 Methyl parahydroxybenzoate 0.4 Purified water 58.3 ( C) Fragrance 0.1 (D) acylated alkali-treated collagen solution (5% by weight) 10.0 Proteoglycan extract (1% by weight) Emulsify. At 70 ° C add (C), at 40 ° C add (D) and cool to 30 ° C with stirring.

Example-3 Neck Emulsion (A) Squalane 3.0 Microcrystalline Wax 2.0 Vaseline 1.0 Stearyl Alcohol 0.7 Sorbitan Monostearate 1.2 Sorbitan Sesquioleate 1.3 Polyoxy Ethylene (20) sorbitan monooleate 2.2 (B) 1,3-butylene glycol 5.0 Methyl parahydroxybenzoate 0.2 Purified water 38.3 (C) Fragrance 0.1 (D) Acylated alkali treatment Collagen liquid (0.1% by weight) 30.0 Proteoglycan extract (10% by weight) 15.0 (A) and (B) are each brought to 80 ° C. and emulsified while stirring. At 70 ° C add (C), at 40 ° C add (D) and cool to 30 ° C with stirring.

Example-4 Pack (A) Carboxymethyl Cellulose 1.5 1,3-Butyleneglycol 5.0 Glycerin 4.0 Ethyl Alcohol 4.0 Polyoxyethylene (20) Lauryl Ether 0.5 Fragrance 0.1 Purified water 78.9 (B) Acylated alkali-treated collagen solution (1% by weight) 5.0 Proteoglycan extract (5% by weight) 1.0 (A) was brought to 60 ° C, (B) was added, and Cool with stirring to ° C.

[0026]

The cosmetic of the present invention is a cosmetic excellent in physical stimulation by blending the acylated alkali-treated collagen and proteoglycan.

Next, the effects of the present invention are shown in the results of a skin irritation test using powder (physical irritation).

[0028]

[0029]

[0030]

[0031]

Skin irritation test by powder (physical irritation)

(Test Method) Using female monitors (35 to 58 years old) as subjects, Example 2a to Example-2g, Comparative Example-2x, Comparative Example-2, 10 persons per group.
The neck cream of y was used on the flexed side of the forearm, to which amorphous alumina was applied as a physical stimulant. For the test of skin irritation, a patch test (Kawai method) by microscopic observation was used, and the test results were all shown as numerical values of B irritation, and compared with a site where no neck cream was used. The neck creams of Examples -2a to -2g are creams in which the amounts of the (a) acylated alkali-treated collagen and (p) proteoglycan of Example 1 are as follows. (A) (P) Example-2a 0.0005 0.0 Example-2b 0.0 0.0005 Example-2c 0.0005 0.0005 Example-2d 0.005 0.005 Example-2e 0.05 0.05 Example-2f 0.1 0.5 Example-2g 0.5 2.0 The neck cream of Comparative Example-1x was prepared by combining the acylated alkali-treated collagen and proteoglycan of Example-1 with hyaluronic acid. The acid was used and the others were similarly prepared. The neck cream of Comparative Example-1y was prepared in the same manner as in Example-1, except that the acylated alkali-treated collagen and proteoglycan were water-soluble collagen.

(Test Results) The results of skin irritation test skin by powder (physical irritation) are shown in Table-3. As is evident from Table 3, Examples 2d to 2g of the present invention correspond to Example 2
a to 2c, Comparative Examples -2x, elongation of the skin compared to 2y,
It is a new cosmetic with excellent elasticity. In addition, also about Example-2, Example-3, and Example-4, the result which was excellent in the protection of the skin was obtained.

[0035]

[0036]

[0037]

[0038]

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Jiro Kitahara 2-130 Tomicho, Nishi-ku, Nagoya-shi Within Menard Cosmetics Co., Ltd. Central Research Laboratory (72) Inventor Satoru Nakata 2-130 Tomicho, Nishi-ku, Nagoya-shi (56) References JP-A-3-106807 (JP, A) JP-A-4-95010 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00 A61K 7/48

Claims (2)

(57) [Claims] 1. An acylated alkali-containing collagen containing 0.005 to 0.5% by weight as solids and proteoglycan at 0.005 to 2.0% by weight as solids, and an acylated alkali.
Compounding ratio of treated collagen and proteoglycan is 100: 1 ~
A cosmetic that protects the skin from physical stimuli that are 1:50 . 2. An acylated alkali-treated collagen and protein.
The cosmetic according to claim 1, wherein the blending ratio of oglycan is from 1: 1 to 1: 5 .