JPH0495010A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic, containing an extract solution of proteoglycan that is a complex of mucopolysaccharides which are extracts from living bodies and proteins, capable of preventing humectant action and moisture loss and excellent in conditioning effects on smoothing of the skin and hair. CONSTITUTION:A cosmetic, containing 0.01-50.0wt.% proteoglycan extract with <=10wt.% solid content and useful as toilet water, cream, milky lotion, pack, rinse, etc. The aforementioned cosmetic is capable of preventing humectant action and moisture loss and excellent in conditioning effects on smoothing of the skin and hair with excellent improving action on extension and resilience of the skin together by blending the proteoglycan extract solution of a complex of mucopolysaccharides and proteins therein.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel cosmetic. More specifically, it contains proteoglycan extract as an active ingredient, which has excellent moisturizing and conditioning effects that prevent water loss and smooth the skin and hair.It also has an anti-aging effect that improves skin elongation and elasticity. Regarding cosmetics.

[Conventional technology]

To moisturize the skin and hair and prevent moisture loss, cosmetics contain polyhydric alcohols such as glycerin, 1,3-butylene glycol, isoprene glycol, polyethylene glycol, and sorbitol, as well as amino acids such as serine, proline, and glutamic acid. are combined. moreover,
Mucopolysaccharides such as hyaluronic acid, sodium chondroitin sulfate, and dermatan sulfate are also included for similar purposes.

On the other hand, for the purpose of improving skin stretch and elasticity, proteins such as water-soluble collagen and elastin, and polypeptides such as hydrolyzed collagen and hydrolyzed elastin are incorporated into cosmetics.

[Problem that the invention seeks to solve]

Under high humidity conditions, polyhydric alcohols such as glycerin and 1,3-butylene glycol function effectively as moisturizers for the skin and hair when incorporated into cosmetics.

However, under low humidity conditions, these polyhydric alcohols conversely absorb moisture from the skin and hair into cosmetics, making them unsuitable as moisturizers for the skin and hair.

When amino acids such as serine, proline, and glutamic acid are incorporated into cosmetics as moisturizing agents, there is a problem in that browning occurs due to the Maillard reaction if sugars are present.

Mucopolysaccharides such as hyaluronic acid and sodium chondroitin sulfate have extremely excellent moisturizing effects as biologically derived moisturizers. However, it cannot be said to be superior to proteins and polypebutites in improving skin stretch and elasticity.

Proteins such as water-soluble collagen and elastin, and polypeptides such as hydrolyzed collagen and hydrolyzed elastin, when incorporated into cosmetics, are excellent in improving skin stretch and elasticity. However, when it comes to moisturizing effects on the skin and hair, there is a lot to be said for! It cannot be said that it is superior in comparison to M class.

Therefore, attempts have been made to combine mucopolysaccharides such as hyaluronic acid and chondroitin sulfate with proteins such as collagen in order to obtain cosmetics that have both a moisturizing effect and an effect of improving skin elongation and elasticity.

However, when mucopolysaccharides and proteins are combined in cosmetics, there is a problem with the stability of the white cast iron, so it has not been put to practical use.

[Failure to solve the problem]

In view of these circumstances, the present inventors have conducted extensive research and found that a cosmetic containing an extract of proteoglycan, which is a complex of biological extracts mucopolysaccharide and tacibacterium, is
Excellent conditioning effect that moisturizes and prevents moisture loss, smooths the skin and hair, and helps to stretch the skin.
It was discovered that the material also has the effect of improving elasticity, and the present invention was completed.

That is, the present invention is a cosmetic characterized by containing a proteoglycan extract. The amount of the proteoglycan extract used in the present invention is 0.001 to 70.0% by weight of the entire cosmetic composition, with a solid content of 10% by weight or less. Preferably it is 0.01 to 50.0% by weight. If it is less than 0.001% by weight, the effects referred to in the present invention cannot be sufficiently exhibited, which is not preferable. It is also possible to incorporate 70.0% by weight or more, but no significant increase in effectiveness is observed, the feeling of use becomes poor due to stickiness, etc., and it is not economical.

The proteoglycan extract used in the present invention is an extract of a mucopolysaccharide protein complex.

A mucopolysaccharide-protein complex consists of several or dozens of mucopolysaccharide chains bonded to the polypeptide chain of a protein.
It is an acidic mucopolysaccharide-protein complex containing 50% protein and having a molecular weight of 1 million to 10 million. The constituent acidic mucopolysaccharides mainly include hyaluronic acid, chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, chondroitin-6.9-sulfate, chondroitin, heparin, heparitin sulfate, and keratosulfate.

This proteoglycan extract is extracted from connective tissue of higher animals. More specifically, it is extracted from the sherry-like intercellular space that fills the spaces between many tissue cells, the intercellular cement, cartilage, keys, skin, and sexual fluid that lubricates the joints.

The manufacturing method involves, for example, removing connective tissue from a cow under water cooling, removing blood vessels, fat, etc., and then slicing it. next,
4M adjusted to pH 5°8 with 0.05M sodium acetate
Extraction is performed with guanidine hydrochloride.

After extraction, centrifugation is performed, and the resulting supernatant is added to 10 times the amount of 0.05M sodium acetate (pH 5,8) under cold temperature for 1 hour.
After dialysis for 2 hours, density gradient ultracentrifugation (25,OOOr
pm, 20°C, 45 hours). If necessary, a preservative is added to the bottom of this fraction. This proteoglycan extract has a protein content of 0.35 to 0.65% by weight, and a solid content of 10% by weight or less.

Incidentally, the cosmetic composition of the present invention can be produced according to a conventional manufacturing method. In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain various ingredients commonly used in cosmetics, such as oils and fats, waxes, hydrocarbons, fatty acids, and alcohol, if necessary, within a range that does not impair the effects of the present invention. esters, synthetic esters, surfactants, humectants, thickeners, inorganic substances, fragrances, drugs, purified water, etc. can be blended.

〔Example〕

Next, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited thereto. In addition,
The proteoglycan extract blended in the following examples is:
It was prepared according to the manufacturing method described above.

Example-1 Makeup Water ■ Ethyl alcohol 10.0 ■ Fragrance appropriate amount ■ Polyoxyethylene (20) hydrogenated castor oil 0.4 ■ Glycerin
5. 0 ■ 1,3-butylene glycol 4.0 ■ Proteoglycan extract 10.0 ■ Methyl roseoxybenzoate
0.1 ■ Citric acid
0. 5■ Sodium citrate
0.3 [Phase] Add purified water to make the total volume 100.

Dissolve ■ to ■ in ■ to obtain A. Next, ① to ② are dissolved in [phase] to obtain B. After adding A to B, No.
, filter through 5C filter paper.

Example-2 Cream ■ Squalane 5. 0 ■ Behenyl alcohol 1.5 ■ Cetyl alcohol 0.5 ■ Polyoxyethylene (20) Sorbitan monostearate 1.2 ■ Sorbitan monostearate 0.8 ■ Polyoxyethylene (20) Cetyl ether 2.5C adsorption purified lanolin 4 .0 ■ Myristyl octyldodecanol 8.0 ■ Stearic acid
1.2@1.3-butylene glycol 6.00 Methyl paraoxybenzoate
0.40 Proteoglycan extract 5.0 [Phase] Flavor Appropriate amount 0
① to ② were dissolved at 80°C to make the total amount 100 with purified water and designated as A. [Phase] Dissolve ■ in ■ at 80°C to obtain B. Emulsify B into A without stirring, add ■ at 70℃, add 0 at 40℃, 3
Cool with stirring to 0°C.

Example-3 Emulsion ■ Squalane 3.0 ■ Microcrystalline wax 2.0 ■ Vaseline 1.0 ■ Stearyl alcohol 0.7 ■ Sorbitan monostearate 1.2 ■ Sorbitan sesquioleate 1.3 ■ Poly Oxyethylene (20) Sorbitan monooleate 2.2■1,3
-Butylene glycol 5.0 ■Methyl paraoxybenzoate 0. 2 [Phase] Proteoglycan 30.00 Flavor
Dissolve (1) to (2) at 80°C with an appropriate amount of purified water to make the total amount 100, and refer to A. ■、■ to @80
Dissolve at ℃ and call it B. Emulsify A with B while stirring,
Add ■ at 70℃, add [phase] at 40℃,
Cool to 30°C with stirring.

Example - 4 packs ■ Carboxymethyl cellulose ■ 1,3-butylene gtycol ■ Glycerin ■ Nithyl alcohol ■ Polyoxyethylene (20) Lauryl ether 0゜ ■ Fragrance ■ Proteoglycan extract ■ Appropriate amount to make the total volume 100 with purified water 0゜■ ~ ■ Dissolve in ■ at 60℃, add ■, and heat to 30℃
Cool while stirring until cooled.

Example-5 Rinse ■Monostearyl chloride Trimethylammonium ■Chloride resin Le.

Dimethylammonium 1.5 ■ Cetyl alcohol 2.5 ■ Behenyl alcohol 0. 5■Liquid paraffin 370 5.0■Glycerin
5.0 ■ Methyl paraoxybenzoate
0. 2■Fragrance
Appropriate amount ■ Proteoglycan extract
3.0 [Phase] Dissolve (1) to (2) at 75°C to make the total amount 100 with purified water to obtain A. Dissolve ■ and ■ in [phase] at 70℃ and make B.a Emulsify B with A while stirring, add ■ at 60℃, add ■ at 40℃,
Cool to 30°C with stirring.

〔Effect of the invention〕

By incorporating proteoglycan abstraction of mucopolysaccharide protein complex, the cosmetic of the present invention has a moisturizing effect, prevents water loss, has a conditioning effect that smooths the skin and hair, and has a conditioning effect that makes the skin stretch. It is a cosmetic that also has an excellent effect on improving elasticity.

Next, regarding the effects of the present invention, a moisture retention test, a human skin elongation and elasticity improvement test, and a long-term use test were conducted.

The results are shown below.

(Water retention test) Test method: 8 female monitors per group (19 to 53 years old)
, apply the lotion of Example-1, Example-1a to Example-1e, Comparative Example-1, and Comparative Example-2 to the pressure side of the forearm,
The electrical conductivity after 10 minutes, 30 minutes, 60 minutes, 120 minutes, and 180 minutes was measured using a high-frequency impedance meter Model 1 manufactured by I, B, and S companies. Measured with IB 352.

Example-1a to Example-1e Lotion of lotion Example-1 Proteoglycan content (%) in which the proteoglycan extract was blended as follows Example-1a O,005 Example -1b 0.02 Example-1c 5.0 Example-1d40.0 Example-1e 60.0 Comparative example-1 crude water Lotion ■ Proteoglycan of Example-1 is 13-butylene glycol, etc. was prepared similarly.

Comparative Example 2 Lotion The lotion of Example 1 was prepared in the same manner except that the proteoglycan was replaced with hydrolyzed collagen.

Test Results The results of the water retention test are shown in Table-1. As is clear from the results in Table 1, Example 1 containing proteoglycan
, Example-1b, Example-IC1 The lotion of Example-1d had excellent water retention and feel when compared to the lotion of Example-18, Comparative Example 1, and Comparative Example-2. This is a new cosmetic.

In addition, similarly excellent water retention results were obtained for Example-2, Example-3, and Example-4.

(Skin elongation and elasticity test) Civilian-1 On the other hand, 40 female monitors (24 to 52 years old) were tested in Example-2.
, Example-2a to Example-2e, Comparative Example-3, Comparative Example-
Cream No. 4 was applied once a day to the flexor side of the forearm for one month. One month later, the elongation and elasticity of the skin were measured using a cutometer SEM474 manufactured by Nippon Nirochiku Co., Ltd.

Example-2a to Example-2e Cream Cream with the following blending amount of proteoglycan extract in the cream of Example-2.

Proteoglycan content (%) 0.007 0.15 1.0 40.0 55.0 Example-2a Example-2b Example-20 Example-2d Example-2e Comparative example-3 Example-2 The cream was prepared in the same manner except that the proteoglycan was replaced with glycerin.

Comparative Example-4 The cream of Example-2 was prepared in the same manner except that water-soluble collagen was used as @proteoglycan.

K-Supervisor-1 The results of the skin elongation and elasticity tests are shown in Table 2.

As is clear from Table-2, Example-2, Example-2b, Example-2c, and Example-2d of the present invention are Example-2
This is a new cosmetic that has improved skin stretch and elasticity and has an excellent feel when used compared to Example 2e, Comparative Example 3, and Comparative Example 4. In addition, Example-1, Example-3, Example-
Similarly, excellent improvement in skin stretch and elasticity was obtained for Example 4.

Note in the margin below: The elongation of the skin is the value obtained when suctioned with a cutometer SEM474, and the elasticity is expressed as the degree to which the skin returns to its original state.

(Long-term continuous use test) Test method Female monitors (35 to 58 years old) who suffer from dryness, firmness, sagging, and wrinkled skin were the subjects, and 1 group of 10
The emulsions of Example 3, Examples 3a to 3e, Comparative Example 5, and Comparative Example 6 were used daily for one month. One month later, the degree of improvement in skin dryness, firmness, sagging, and wrinkles was determined based on subjective findings.

Example-3a to Example-3e Emulsion A cream containing the [phase] proteoglycan extract of the emulsion of Example-3 in the following amount.

Proteoglycan content (%) Example-3a 0.005 Example-3b 0020 Example-3c 1.0 Example-3d5.0 Example-3e 60.0 Comparative Example-5 Emulsion Emulsion of Example-3 The [phase] proteoglycan was an aqueous hyaluronic acid solution, and the others were prepared in the same manner.

Comparative Example-6 Emulsion [Phase] The emulsion of Example-3 was prepared in the same manner except that the proteoglycan was water-soluble collagen.

Test Results The long-term continuous use test results are shown in Table 3. As is clear from Table 3, 1 Example-3, Example-3b, Example-3 of the present invention
C1 Example-3d was excellent in improving skin dryness, firmness, sagging, and wrinkles compared to Comparative Example-5 and Comparative Example-6.
This is a new cosmetic that is easy to use.

In addition, in Example-1, Example-2, and Example-4, excellent results were similarly obtained in improving skin dryness, firmness, sagging, and wrinkles.

Margin below Long-term continuous use test results

Claims (2)

[Claims] (1) A cosmetic characterized by containing a proteoglycan extract. (2) The cosmetic according to claim 1, which contains 0.01 to 50.0% by weight of a proteoglycan extract with a solid content of 10% by weight or less.