JPH069422A - Promoter for biosynthesis of hyaluronic acid - Google Patents

Abstract

PURPOSE:To provide a promoter for biosynthesis of hyaluronic acid, containing a remarkably highly safe extract of a sea weed, holey sea lettuce which is a green algae as the active component, capable of preventing senescence of the skin and of making the skin wrinkleless, smooth, wet and young and useful for a cream, a milky lotion, etc. CONSTITUTION:This promoter for biosynthesis of hyaluronic acid contains, as the active component, an extract obtained by extracting holey sea lettuce which is a sea weed belonging to Ulva, Ulvaceae with water, a hydrophilic organic solvent, a hydrous hydrophilic organic solvent, another organic solvent, etc. This extract of holey sea lettuce is excellent in stability in a medical preparation as a dermal medicine for external use and in practical utility. This promoter for biosynthesis of hyaluronic acid exhibits remarkably excellent effects, e.g. activation of the dermal cells, prevention of dermal senescence and wrinkles of the skin and maintenance of the smooth, wet and youthful skin. Since this promoter can also keep the wetting properties of an articulation, this promoter is also useful as a medicine for improving an articulation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention is extremely safe.
A biological hyaluronic acid synthesis promoter consisting of a green alga plant extract, which is blended with it to prevent skin aging and to give a smooth, moisturizing, cosmetic cream, emulsion skin, etc. External preparations and the like can be obtained.

[0002]

2. Description of the Related Art Hyaluronic acid is used for skin, ligaments, synovial fluid,
It is widely distributed in the living body such as the vitreous body of the eye. For example, in the skin, it protects cells, transports nutrients, retains tissue moisture, maintains flexibility, and maintains tissue structure and function as synovial fluid and retains lubricity. , Plays an important role.

On the other hand, when the cell function is deteriorated due to the aging of the skin or joints or the pathological state, the amount of such hyaluronic acid in the living body is decreased, and as a result, the dryness of the skin, rough skin, firmness and elasticity are reduced. It causes joint pain and the like due to increase in spots and wrinkles, or deterioration of wettability of joints.

In order to improve such a condition, a method of applying a cosmetic containing hyaluronic acid, a biological component such as a natural moisturizing factor, or the like to the skin, or directly injecting hyaluronic acid into a joint is used. However, even if such a substance is given from the outside, the fundamental function is not improved and a sufficient effect cannot be expected. Especially hyaluronic acid is hardly absorbed through the skin. Therefore, instead of simply supplementing from the outside, by utilizing the self-recovery power of the body that humans originally possess, by promoting the hyaluronic acid synthesizing ability of the cells themselves, a substance that fundamentally improves the function of the body Development is expected.

Hyaluronic acid is produced by epidermal basal cells, fibroblasts, synovial cells, etc. Among them, hyaluronic acid synthesized by epidermal basal cells improves water retention, protects cells, supplies nutrients, and abolishes. By being involved in enhancing excretion of substances, it plays an important role in making the skin healthy, improving the firmness and tone of the skin, and making the texture finer. Therefore, the epidermal hyaluronic acid synthesis promoter is very useful for the health of the skin, and is attracting attention as a wrinkle improving agent. Although such an effect is known for retinoic acid, it cannot be used as an active ingredient in general medicines, quasi drugs, and cosmetics because it has side effects such as strong skin irritation and skin thickening. Therefore, development of an active ingredient having high safety and fundamentally improving skin function is desired.

[0006]

DISCLOSURE OF THE INVENTION The present invention provides a preparation which exhibits an excellent biohyaluronic acid synthesis promoting effect and is excellent in safety.

[0007]

Means for Solving the Problems In order to solve the above problems, the present inventors have screened various plant extracts with an effect of promoting hyaluronic acid synthesis on epidermal cells of rats as an index. It was found that the desired effect could be obtained in the extract of the specific plant to which it belongs, and the present invention was completed.

[0008] On the other hand, this effect is observed in conventional seaweed extracts which are considered to be good for the skin such as enhancing the moisturizing property, for example, extracts of brown algae such as kelp, Honda straw, Kajime, hagwort, and green algae such as human algae. However, it was specific to the extract of Anahusa, a plant of the genus Ulva of the family Ulva.

That is, the biohyaluronic acid synthesis promoter of the present invention is characterized by containing, as an active ingredient, an extract of sea lettuce, which is a seaweed plant belonging to the genus Ulva genus Ulva.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The Ulva family (Ul used in the present invention
The seaweed plant Ulva pertusa of the genus Ulva is a membranous body with large and small holes as it grows. During the war, this seaweed was dried and minced on rice. Anahusa grows on rocks in the lower intertidal zone and is widely distributed along the coasts of various parts of Japan.

When the substance of the present invention is extracted, the blue-green alga may be in a non-dried state, but it is preferable from the viewpoint of extraction efficiency that it is dried by a method such as air drying or freeze-drying before being subjected to extraction. The method for extracting the substance of the present invention is not limited,
An ordinary extraction method is adopted, and water, hydrophilic organic solvent, water-containing hydrophilic organic solvent, other organic solvent and the like are used to extract from Anophelium chinensis.

Examples of the hydrophilic organic solvent include lower alcohols such as methanol, ethanol and isopropanol, acetone, methyl ethyl ketone, acetonitrile, dimethyl sulfoxide and dimethylformamide.
In particular, it is preferable to perform extraction using water or a mixture of water and a lower alcohol such as methanol, ethanol or isopropanol. In that case, the ratio of water to lower alcohol is such that lower alcohol / water is 0/100 to 70/30.
(V / V, volume ratio) is preferable, and 0/100 to 40/60 is more preferable.

The ratio of dried Anahusa to the extraction solvent is
The dried sardine / solvent ratio is preferably in the range of 1/50 to 1/2. Moreover, as other extraction conditions, the extraction temperature is not particularly limited, but is 5 to 80 ° C., preferably 5 to 50 ° C.
It is preferable to perform the stirring at a temperature in the range of 1 to 24 hours, and the extraction pH is not particularly limited as long as it does not tend to be extremely acidic or alkaline. It is also possible to increase the extraction efficiency by repeating the extraction operation on the extraction residue generated by the above extraction operation.

Further, after extraction by the above method, ultrafiltration,
It is preferable to obtain a fraction having a molecular weight of 3,000 or more or a fraction having a molecular weight of 100,000 or less by using physical methods such as gel filtration alone or in combination.
More preferably, a fraction having a molecular weight of 3,000 to 100,000 is obtained. The extract may be used as it is, or may be a diluted solution or a concentrated extract.
It may be made into a dry powder by freeze-drying or the like, or may be prepared as a paste.

When it is prepared as a dry powder, it is used by dissolving it in water or a lower alcohol containing water such as methanol, ethanol and isopropanol in advance, or by solubilizing it in an external composition containing water described later. Is preferred. INDUSTRIAL APPLICABILITY The biological hyaluronic acid synthesis promoter of the present invention has an excellent biological hyaluronic acid synthesis promoting effect and is highly safe, and therefore can be used in various applications such as pharmaceuticals, quasi drugs, and cosmetics.

When used as an external preparation for the skin, the extract of Anemarrhena as an essential component can be blended in any concentration,
Usually, 0.01 to 30% by weight (hereinafter abbreviated as%), preferably 0.1 to 10%, and particularly preferably 0.2 to 5% are contained in various skin external preparations.

In addition to the above-mentioned essential components, the skin external preparation containing the extract of Anemarrhena as an essential component of the present invention, in addition to the above-mentioned essential components, is a raw material usually used for external preparations such as surfactants, oils, alcohols, humectants, Thickeners, antiseptics, antioxidants, chelating agents, pH adjusters, fragrances, dyes, ultraviolet absorbers / scatterers, vitamins, amino acids, water and the like can be added.

Specifically, the surfactants include lipophilic glycerin monostearate, self-emulsifying glycerin monostearate, polyglycerin monostearate, sorbitan monooleate, polyethylene glycol monostearate, polyoxyethylene sorbitan. Monooleate, polyoxyethylene cetyl ether, polyoxyethylene sterol, polyoxyethylene lanolin,
Nonionic surfactants such as polyoxyethylenated beeswax and polyoxyethylene hydrogenated castor oil; sodium stearate, potassium palmitate, sodium cetyl sulfate, sodium lauryl phosphate, triethanolamine palmitate, sodium polyoxyethylene lauryl phosphate, Examples thereof include anionic surfactants such as sodium N-acylglutamate; cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; amphoteric surfactants such as alkylaminoethylglycine hydrochloride liquid and lecithin. .

As the oil component, vegetable oils such as castor oil, olive oil, cacao oil, camellia oil, palm oil, wax, jojoba oil, grape seed oil, avocado oil and the like; animal oils and fats such as mink oil and egg yolk oil; beeswax , Whale wax, lanolin, carnauba wax, candelilla wax, and other waxes; liquid paraffin, squalane, microcrystalline wax, ceresin wax, paraffin wax, hydrocarbons such as vaseline; lauric acid, myristic acid, stearic acid, oleic acid, Natural and synthetic fatty acids such as isostearic acid and behenic acid; cetanol, stearyl alcohol,
Examples thereof include natural and synthetic higher alcohols such as hexyldecanol, octyldodecanol, and lauryl alcohol; esters such as isopropyl myristate, isopropyl palmitate, octyldodecyl myristate, octyldodecyl oleate, and cholesterol oleate.

As moisturizers, polyhydric alcohols such as glycerin, propylene glycol, 1,3-butylene glycol, sorbitol, polyglycerin, polyethylene glycol and dipropylene glycol; amino acids, sodium lactate, sodium pyrrolidonecarboxylate and the like N
Examples of the MF component include water-soluble polymer substances such as hyaluronic acid, collagen, mucopolysaccharide, and chondroitin sulfate.

As the thickener, sodium alginate,
Natural polymer substances such as xanthan gum, aluminum silicate, quince seed extract, tragacanth gum, starch; semi-synthetic polymer substances such as methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, soluble starch, cationized cellulose; carboxyvinyl polymer, polyvinyl alcohol, etc. The synthetic polymer substance and the like can be exemplified.

The preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 3,4,4'-. Trichlorocarbanilide, benzalkonium chloride, hinokitiol,
Examples thereof include resorcin, ethanol and the like.

Antioxidants include dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, and ascorbic acid; chelating agents include disodium edetate, ethylenediaminetetraacetic acid salt, pyrophosphate salt, hexametaphosphate salt, and citrate salt. Acid, tartaric acid, gluconic acid, etc .; as a pH adjusting agent, sodium hydroxide,
Examples include triethanolamine, citric acid, sodium citrate, boric acid, borax, potassium hydrogen phosphate and the like.

Examples of the ultraviolet absorbing / scattering agent include 2-hydroxy-4-methoxybenzophenone, octyldimethylparaaminobenzoate, ethylhexylparamethoxycinnamate, titanium oxide, kaolin, talc and the like.

As vitamins, vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin U, carnitine, ferulic acid, γ-oryzanol, α-lipoic acid, orot Examples thereof include acids and their derivatives.

Amino acids include glycine, alanine, valine, leucine, isoleucine, serine, threonine, phenylalanine, tyrosine, tryptophan,
Examples include cystine, cysteine, methionine, proline, hydroxyproline, aspartic acid, glutamic acid, arginine, histidine, lysine and their derivatives.

The optional components are not limited to these. By appropriately blending the above-mentioned essential components and optional components, for example, the Anopheles mori extract 0.01 to 30
%, As an optional component oil content 0 to 80%, surfactant 0-1
It is possible to provide a skin external preparation containing 2%, a humectant 1 to 15%, a purified water balance, and a trace amount of a preservative. Specifically, it can be used in various product forms such as cream, milky lotion, lotion, beauty essence, pack, undermake-up, foundation, jelly, ointment and the like.

A specific example of the case of using as a skin cosmetic is as follows. (1) Cream for skin Anahusa extract 0.1-10%, oil content 20-70%,
A composition containing 2 to 7% of a surfactant, 1 to 10% of a moisturizing agent, a balance of purified water, a trace amount of a preservative, and a trace amount of a fragrance.

(2) Milky lotion Anahusa extract 0.1-10%, oil content 10-40%,
A composition containing 0 to 15% of alcohols, 1 to 5% of a surfactant, 1 to 10% of a moisturizing agent, 0 to 2% of a thickener, a balance of purified water, a trace amount of a preservative, and a trace amount of a perfume.

(3) Toner lotion, beauty essence, Anahusa extract 0.1-10%, alcohols 5-2
0%, surfactant 0-2%, moisturizer 2-8%, thickener 0
~ 2%, antioxidant 0-0.5%, chelating agent 0-0.
A composition containing 1%, a pH adjusting agent of 0 to 0.2%, a purified water balance, a preservative trace amount, a coloring matter 0 to trace amount, and a perfume trace amount.

(4) Packing agent Anahusa extract 0.1 to 10%, alcohols 2-1
A composition containing 0%, a humectant 2 to 10%, an inorganic powder 0 to 20%, a film forming agent 10 to 20%, a purified water balance, a preservative trace amount, and a fragrance trace amount.

Although the details of the mechanism by which the extract of the present invention exerts an excellent anti-aging effect on the skin is unknown, the biological hyaluronic acid synthesis promoter contained in the extract of Anemarrhena asiatica is hyaluronan of skin cells. It is presumed that by activating the acid synthesizing ability, the moisturizing property, flexibility and elasticity of the skin are improved and a remarkable effect of preventing skin aging is exhibited.

Furthermore, it was confirmed that the Anopheles mellifera extract of the present invention is excellent in stability as a skin external preparation in the preparation and is highly practical. In addition, when the safety of the blue-green alga extract used in the present invention was confirmed, acute toxicity, skin irritation,
In terms of skin sensitization, there was no particular problem in practical use, and it was confirmed that the safety was high.

[0034]

EFFECTS OF THE INVENTION According to the present invention, by virtue of the biological hyaluronic acid synthesis promoting action, skin cells are activated and skin aging is prevented, wrinkles are prevented, and smooth, moist and youthful skin is obtained. A remarkably excellent effect that can be given is obtained, and by mixing this, a highly safe external preparation for skin is provided. Further, it maintains the wettability of the joint and can be used as a therapeutic agent for the joint.

The external preparation for the skin containing the extract of Anahinosa of the present invention is widely used in various forms such as various cosmetic creams, emulsions, lotions, beauty essences, packs, undermake-ups, foundations, jellies and ointments. Can be used. Next, the present invention will be described with reference to examples, but the present invention is not limited thereto.

[0036]

【Example】

Example 1 100 g of a dried product of Anopheles columbine was immersed in 10 times amount of 30% ethanol overnight at room temperature for extraction twice, and then concentrated and freeze-dried to obtain 25 g of an anoha lettuce extract. In addition, in the same manner, seaweeds other than Anaaosa (Obermoku, kelp, Honda straw, Kajime,
An extract of (Human Exa) was obtained.

Next, keratinocytes were isolated from the skin of neonatal (3-day-old) rats by trypsin treatment, cultured in a growth medium, and then cultured in a differentiation medium for 2 days. The seaweed extract described above was added to the cells at 20 μg / ml to 500 μm.
After being allowed to act for 48 hours at a concentration of μg / ml, ³ H-glucosamine (5 μCi / dish) was added for 24 hours.
Incubated for hours. After the medium was treated with actinase to digest the protein, the total amount of ³ H containing hyaluronic acid released into the medium was determined by the cetylpyridium chloride precipitation method.
-GAG was separated and its radioactivity was measured, and the ability to promote GAG synthesis when the control was set to 1 (control ratio:
Magnification) was calculated. The results are shown in Table 1. GAG synthesis accelerating ability (magnification) = A / BA A: ³ H-GAG radioactivity (d) when each seaweed was added (d)
pm) B: Radioactivity of ³ H-GAG without addition (dpm)

As shown in the results shown in Table 1, the effect of promoting GAG synthesis was not observed in the brown algae of kelp, Honda straw, Kajime, and obamoku, and of the green algae of human exa. On the other hand, Anahusa promoted GAG synthesis in a concentration-dependent manner, and at a particularly high concentration, a high effect of at least twice that of the control was observed.

[0039]

[Table 1]

Subsequently, the total GAG obtained from the control (without seaweed added) and the medium in which 500 μg / ml of Anahasa was made to act was applied to a Sepharose CL-6B column (1.1 cmφ × 60 cm) to give 0.15 M-
50 mM Tris-HCl buffer containing NaCl (pH 7.
Development using the developing solution of 4) (flow rate: 0.37 ml / mi
n, room temperature). One fraction of 1.04 ml was collected and the radioactivity of each fraction was measured to calculate the radioactivity of hyaluronic acid and sulfated GAG in total GAG. The radioactivity of each fraction is shown in FIGS.
Fraction No. 22 to 29 are hyaluronic acid fractions, 30 to 57 are sulfated GAG fractions, and the total sum of the fractions of each fraction and the control ratio are shown in Table 2. Table 2
As shown in the results, the amount of hyaluronic acid synthesized in total GAG increased 4.8 times as much as the control when 500 μg / ml of Anahusa was acted, and it was confirmed that Anahusa has a high stimulation of epidermal hyaluronic acid synthesis. It was

[0041]

[Table 2]  FractionTotal GAG hyaluronic acid Sulfated GAG Radioactive control Radioactive control Radioactive control Specimen (dpm) Roll ratio (dpm) Roll ratio (dpm) Roll ratio Control 83905-15525-68380-Anaosa 205161 2.45 74104 4.77 131052 1.92 (500 μg / ml)

Example 2 A group of 6 hairless mice was prepared, and a hydrophilic ointment having the composition shown in Table 3 containing 5% of the test substance was prepared, and 0.2 g of each was applied once daily to the back skin for 7 days. did. After slaughter, the epidermis at the application site was separated, degreased and dehydrated, and then treated with actinase. After deproteinization, the separated hyaluronic acid was quantified by the carbazole / sulfuric acid method. The test substance used was that prepared in Example 1. Further, the amount of hyaluronic acid was expressed as a weight (average value) per unit area of skin tissue as a relative value with respect to the case where no test substance was added.

[0043]

[Table 3] Ingredient Amount (wt%) white petrolatum 25.0 Stearyl alcohol 20.0 Polyoxyethylene (60) hardened castor oil 4.0 Glycerin 1.0 Propylene glycol 12.0 Methylparaben 0.1 Propylparaben 0.1 analytes 5.0 Purified water balance monostearate

[0044]

[Table 4] Test substance Quality of epidermal hyaluronic acid (relative value) No addition (control) 100 Anguilla versicolor extract 130 Human ragweed extract 101 Leekweed extract 98 Kombucha extract 100 Honduala extract 99 Kajime extract 98

From the results shown in Table 4, in the group to which the hydrophilic ointment containing the extract of Ana sarcoma as the active ingredient of the present invention was applied, the amount of hyaluronic acid in the epidermis was obviously higher than that in the control group. On the other hand, in the other seaweed extract-containing hydrophilic ointment applied groups, a significant increase in the amount of epidermal hyaluronic acid was not observed as compared with the control group.

Example 3 Six animals each in the control group and the group to which the blue-green alga extract was applied were subjected to an experiment under the same conditions as in Example 2, after slaughter,
Make a tissue section of the skin of the application site and use hematoxylin /
After eosin staining, the skin structure was examined. As a result, the skin tissues of the Anahusa extract-applied group in any of the individuals were clearly enriched and activated in the epidermal tissue as compared with that of the control group, and in particular, the keratinocytes of the basal layer of the epidermis were regularly arranged. all right.

Representative examples are shown in FIGS. Fig. 3: Photograph of the skin tissue of the control group Fig. 4: Schematic diagram of the epidermis part Fig. 5: Photograph of the skin tissue of the anaosasa application group Fig. 6: Schematic diagram of the epidermal part The above results are the active ingredients of the present invention Anaasosa It is shown that by applying the hydrophilic ointment containing the extract, the epidermal keratinocytes were activated and the synthesis of hyaluronic acid was promoted, and as a result, the aging of epidermal tissue was improved.

Example 4 To 3.0 kg of dried Anabaena serrata, 20 times amount of water was added, and the operation of stirring for 24 hours at 5 ° C. was repeated twice, and then the extracts were combined and concentrated under reduced pressure by an evaporator. . Then, the concentrated solution was passed through an ultrafiltration membrane (Amicon YM100) having a molecular weight cutoff of 100,000 to obtain a molecular weight of 100,
Fractions below 000 were obtained. Next, this fraction was passed through an ultrafiltration membrane (Amicon YM3) having a molecular weight cut off of 3,000,
Fractions with a molecular weight of 3,000 or less are removed to give a molecular weight of 3,000
Fractions from 100,000 to 100,000 were collected and freeze-dried to obtain 150 g of a Bromeliad extract.

Next, the components 1 to 7 and 8 to 1 shown in Table 5 are shown.
After mixing and dissolving 1 separately, while stirring the solutions of the components 8 to 11, the solutions of the components 1 to 7 are added thereto to emulsify, and after cooling, the component 12 is added on the way and cooled to room temperature. Then, the creams shown in Table 5 were prepared. The numbers indicate the blending amount (% by weight) (hereinafter the same). In addition, POE (2
0) indicates polyoxyethylene and the number of moles thereof added.

[0050]

[Table 5] Ingredients present invention product Comparative Example 1 Liquid paraffin (# 70) 5.0 5.0 2 Squalane 14.0 14.0 3 cetostearyl alcohol 6.0 6.0 4 Beeswax 2.0 2.0 5 glycerol monostearate 2.0 2.0 6 POE (20) sorbitan monolaurate 2.0 2.0 7 Propylparaben 0.1 0.1 8 Anahusa extract 0.5-9 Diglycerin 5.0 5.0 10 Methylparaben 0.2 0.2 11 Purified water Balance Balance 12 Perfume Trace Trace

The effectiveness of the cream thus prepared was evaluated as follows. Twenty women (30 to 50 years old) who are statistically equivalent are selected, and twice a day (morning and night),
After three consecutive months, the present invention product and the comparative example were separately applied to the left and right faces by the half face method, and then fine wrinkles and skin quality (gloss, gloss,
The degree of improvement in moisture was examined. The results are shown in Table 6.

[0052]

[Table 6] Table 6: Evaluation results The present invention product is the same as the present invention product Comparative example is comparative example Comparative item is good Evaluation item Good Improvement Improvement 3 7 8 2 0 skin type Good Good wrinkles 5 8 6 1 0

From the results shown in Table 6, the cream of the present invention containing the extract of Anahusa showed a remarkable effect of improving small wrinkles and an effect of improving the skin quality, as compared with the cream containing no extract. The above results show that the external application of the cream containing the active ingredient of the present invention promoted the synthesis of hyaluronic acid in skin cells and remarkably improved the aging symptoms of skin. No abnormalities such as redness were observed in the skin condition during and after the use of the cream for 3 months.

Example 5 Components 1 to 6 shown in Table 7 were sequentially added to Component 7 and dissolved, and 8 was added to prepare a lotion shown in Table 7.

[0055]

[Table 7] Ingredients present invention product Comparative Example 1 Ulva pertusa extract 0.1 - 2 1,3-butylene glycol 3.0 3.0 3 Citric acid 0.02 0.02 4 Sodium citrate 0.05 0.05 5 Ethanol 18.0 18.0 6 Methylparaben 0.1 0.1 7 Purified water Balance Balance 8 Perfume trace trace

As the extract of the component Anana Ulva, the one prepared in Example 4 was used (the same applies to the following Examples). The effectiveness of the lotion thus prepared was examined in the same manner as in Example 4. The results are shown in Table 8.

[0057]

Table 8 Table 8: evaluation results the product of the present invention is an evaluation item good Good comparative example is the product of the present invention is the same, such as Comparative Example Improvement of improvement 2 8 8 2 0 skin quality of a little good good fine lines 3 8 7 2 0

From the results shown in Table 8, the lotion of the present invention containing the extract of Anahusa showed a remarkable improving effect on wrinkles and a improving effect on skin quality, as compared with the lotion containing no extract. The above results show that external application of lotion containing the active ingredient of the present invention promoted hyaluronic acid synthesis in skin cells and significantly improved skin aging symptoms. No abnormalities such as redness were observed in the skin condition during and after use of the lotion for 3 months.

Example 6 Components 1 to 4 and components 5 to 9 shown in Table 9 were separately dissolved and then mixed to prepare a beauty essence.

[0060]

[Table 9] Ingredients present invention product Comparative Example 1 Ulva pertusa extract 1.0 - 2 Glycerin 4.0 4.0 3 carboxyvinyl polymer 0.5 0.5 4 Purified water Balance Balance 5 acetate dl-alpha-tocopherol 0.1 0.1 6 Ethanol 10.0 10.0 7 Polyoxyethylene (40) hardened castor Oil 0.5 0.5 8 Methylparaben 0.1 0.1 9 Perfume Trace Trace

The effectiveness of the cosmetic liquid thus prepared was examined in the same manner as in Example 4. The results are shown in Table 10.

[0062]

[Table 10] Table 10: Evaluation results The present invention product is the same as the present invention product Comparative example is comparative example Comparative item is good Evaluation item Good Improvement of improvement 4 9 6 1 0 skin quality of a little good good fine lines 6 8 6 0 0

From the results shown in Table 10, the beauty essence of the present invention containing the extract of Anemarrhenae showed a remarkable effect of improving fine wrinkles and an effect of improving skin quality, as compared with the beauty essence containing no extract. The above results indicate that the external application of the beauty essence containing the active ingredient of the present invention promoted the synthesis of hyaluronic acid in skin cells and significantly improved the aging symptoms of skin. No abnormalities such as redness were observed in the skin condition during and after the use of the beauty essence for 3 months.

Example 7 Components 1 to 7 shown in Table 11 were heated and dissolved at 70 ° C. On the other hand, components 8 to 13 are heated and dissolved at 70 ° C.
After adding (Components 1 to 7) and emulsifying, while cooling,
Component 14 was added midway and cooled to room temperature to prepare emulsions shown in Table 11.

[0065]

[Table 11] Ingredients present invention product Comparative Example 1 Liquid paraffin (# 70) 10.0 10.0 2 Isopropyl myristate 1.5 1.5 3 Glycerin monostearate 0.5 0.5 4 Stearic acid 2.0 2.0 5 POE (20) stearyl ether 0.7 0.7 6 glycyrrhetinic acid 0.1 0.1 7-butyl Paraben 0.1 0.1 8 Anahusa extract 0.05-9 Glycerin 2.0 2.0 10 Carboxyvinyl polymer 0.1 0.1 (Molecular weight 1 million to 1.5 million) 11 Ethanol 10.0 10.0 12 Methylparaben 0.1 0.1 13 Purified water Balance Balance 14 Perfume Trace Trace

The effectiveness of the thus-prepared emulsion was examined in the same manner as in Example 4. As a result, the effect of improving wrinkles and the effect of improving skin quality similar to those in Example 4 were exhibited. No abnormality such as redness was observed in the skin condition during and after the use of the emulsion for 3 months.

[Brief description of drawings]

FIG. 1 is a graph showing the radioactivity of each fraction for a control.

FIG. 2 is a graph showing the radioactivity of each fraction with respect to Ulva persica (500 μg / ml).

FIG. 3 is a cross-sectional photograph showing the structure of skin tissue when a control hydrophilic ointment is used.

FIG. 4 is a schematic diagram of a skin portion of FIG.

FIG. 5 is a cross-sectional photograph showing the structure of skin tissue in the case of using a hydrophilic ointment containing an extract of Anemarrhena asiatica.

FIG. 6 is a schematic view of the epidermis part of FIG.

Claims (1)

[Claims] 1. The seaweed Ulva pertu
A bio-hyaluronic acid synthesis promoter comprising the extract of sa) as an active ingredient.