JPH107518A - Skin lotion - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin lotion having an effect comparable to or exceeding an extract derived from an animal by containing an extract of seeds of a family Leguminosae plant and an extract of a family Lamiacea plant. SOLUTION: This skin lotion contains an extract from seeds of Lupinus albus L. as a family Leguminosae plant and an extract from Thymus vulgaris L. as a family Lamiacea plant as active components. The preparation contains 0.1-50wt.% in total amount of both extracts and is prepared to creamy, an ointmentlike, a milky lotion-like, a gel-like, filmy or a liquid-like. A highly polymerized protein contained in the Lupinus albus L. exhibits a tightening effect for skin and makes the surface of skin smooth by forming a film on the skin. Flavonoid or tannin contained in the Thymus vulgaris L. promotes hydroxylation of proline or lysine important in transforming procollagen to collagen and assists reconstruction of a tissue by an anti-elastin action. Therefore, the preparation exhibits a tightening effect for skin, reducing depth of wrinkle on the skin and further reducing microscopic undulations on the skin, then smoothing the skin.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin,
Particularly, the present invention relates to an external preparation for skin containing a legume plant seed and a Labiatae plant extract as main active ingredients.

[0002]

2. Description of the Related Art There have long been attempts to incorporate a natural extract into an external preparation for skin with an expectation of physiological activity on the skin. Natural materials used for such purposes are mainly animal organs (brain, liver, spleen, etc.) and body fluids
(Blood, etc.) and plant tissues (leaves, stems, roots, flowers, seeds, etc.).

[0003] The physiologically active components contained in the extracts from these natural materials include various phospholipids, vitamins, amino acids, proteins, arbutin, kojic acid, placenta extract, and serum albumin (for example, bovine serum albumin). ,
Glycosaminoglycans, hyaluronic acid, collagen, elastin, polyphenols (for example, tannins), flavonoids and the like are known.

[0004]

However, these extracts elute certain components by treating the dry-preserved material with warm or hot water or various organic solvents.
Most of them are obtained by filtration and optionally fractionation, and the content of the physiologically active substance is low except for a part. In many cases, the essence of the active ingredient is not specified, and the fact that the effect is proven is limited.

[0005] In recent years, there has been a growing trend to convert animal-derived extracts to plant-derived extracts from the viewpoint of animal welfare and the like. However, extracts from animal organs and the like often have high bioactivity, and it is not easy to find an extract that is of plant origin and has an effect comparable to or superior to that of animal origin.
Even if there is a plant resource containing a component exhibiting a useful physiological activity, the useful component may be deteriorated by the above-mentioned general extraction method.

[0006]

[Means for Solving the Problems] At this point, a white banana lupine
( Lupinus albus L.) is an annual legume that is classified in the genus Haupa, and has been cultivated in southern and central Europe for more than 3000 years, with seeds used for food and stems and leaves used for feed. I have. Seeds 11.0% moisture, protein 3
It is reported to contain 2.8%, fat 8.9%, nitrogen-free extract 44.7%, sugar 6.3% and ash 2.6%. Furthermore, the seeds of white lupine contain a large amount of high-molecular-weight storage proteins such as albumin and globulin. On the other hand, Thymus vulga [ Thymus vulga
ris L.)] is a perennial Lamiaceae plant classified in the genus Ibukija, and is a standing shrub. This plant is a typical herb known from Europe for a long time mainly in the Mediterranean coastal region, and has a strong aroma in flowers, shoots and leaves, and is rich in flavonoids and tannins.

The inventors of the present invention have paid attention to the fact that the seeds of Shirovana lupine contain a large amount of useful bioactive components such as high-molecular-weight proteins and sulfur-containing amino acids, and the falcon scutellaria contains many useful bioactive components such as flavonoids and tannins. Investigations were made to use the extract from Escherichia coli as an active ingredient of an external preparation for skin. That is, when the effect of the above extract on the skin was examined, the high-molecular-weight protein contained in Shirovana lupine exhibited an excellent skin tightening effect, and at the same time, had the ability to form a kind of film on the skin. It has been found. The resilient membrane formed in this way makes the skin surface smoother, and the flavonoids and tannins contained in T. chinensis play an important role in converting procollagen to collagen. It has been found that it promotes lysine hydroxylation and promotes tissue remodeling by anti-elastin action, thus providing the beginning of the present invention.

Therefore, an aqueous solution and a cream containing the above-mentioned extract mixture were prepared, and various tests were conducted using healthy volunteers to examine the effect of the extract mixture on skin. As a result, all volunteers answered that the aqueous solution containing this extract had a better skin tightening effect than the aqueous solution containing bovine serum albumin (BSA). In addition, when a skin replica before and after application was prepared and the skin surface was analyzed using a scanning electron microscope, the cream of the present invention had a reduced wrinkle depth compared to before application, and had a smoother appearance. To the skin surface. Furthermore, the above skin replica was imaged using a confocal microscope and subjected to three-dimensional analysis.As a result, the reduction rate of the density of the micro relief of the skin was higher than that of the aqueous solution containing BSA, and the micro relief of the skin was small. (Microrelief) was reduced and the skin was smoothed by smoothing the skin. These effects were demonstrated to be equal to or greater than those of an aqueous solution or cream containing BSA.
Thus, the present invention has been completed.

Accordingly skin external preparation according to the invention the extract and / or an active ingredient extract of Labiatae plants from seeds of leguminous plants, leguminous plants white lupine (Lupinus al
bus L.), and the Labiatae plant is Thymu
s vulgaris L.). The content of the extract depends on the dosage form, but considering the effect and cost, it is appropriate to be about 0.1-50.0% by weight.

[0010] The dosage form of the external preparation for skin according to the present invention can be cream, ointment, emulsion, gel, film or liquid. In the external preparation for skin according to the present invention, "creamy" refers to a substance which is emulsified in an oil-in-water type or a water-in-oil type and has a relatively high viscosity. And inorganic or organic pigments. The term “ointment” refers to an oily vaseline-like state containing almost no water, and this agent may contain an inorganic or organic pigment whose safety is guaranteed. "Emulsion" refers to those that are emulsified in oil-in-water or water-in-oil and have relatively low viscosity and show fluidity. It can have pigments. The term “gel-like” refers to a state of being clear and having low viscosity having fluidity to high viscosity having no fluidity. "Film-like" refers to a state in which the above-mentioned ointment is applied on a substrate so as to form a thin layer. “Liquid” refers to a state close to water that is clear and has little viscosity. Skin external preparations in each of the above states, specific examples of cosmetic dosage forms include skin creams, cosmetic emulsions, lotions, lotions, liquid foundations, pressed powders, lipsticks, lip glosses, and the like. Examples of the hair styling type include shampoo, rinse and treatment. Examples of the pharmaceutical dosage form include cream, petrolatum ointment, gel ointment, poultice, emulsion, plaster and the like.

The external preparation for skin of the present invention can, of course, contain other physiologically active substances as accessory components. The physiologically active substance which is a secondary component is a component contained in an extract conventionally used, and includes various phospholipids, vitamins, amino acids, proteins, arbutin described at the beginning of the present specification. , Kojic acid,
Placenta extract, serum albumin (for example, bovine serum albumin), glycosaminoglycan, hyaluronic acid, collagen, elastin, polyphenol (for example, tannin),
Flavonoids and the like can be mentioned.

[0012]

The present invention will now be described in more detail with reference to Formulation Examples and Test Examples. The appearance and the like of the dried powder of the extract mixture of the extract of the white root lupine and the extract of St. John's wort (hereinafter referred to as the present extract) used in the test examples and preparation examples described below were as follows. Appearance: Brown powder pH: 4.6 (4% aqueous solution) Dry residue: 88.50 (% by weight) Inorganic salt: 11.50 Total nitrogen: 1.36 Flavonoid: 1.70 Polyphenol: 2.90

Formulation Example 1 (Preparation of Aqueous Liquid Preparation) 40 g of the present extract and about 500 ml of purified water were added to a 1000 ml volumetric flask with a stopper, and the extract was dissolved by stirring for about 5 minutes. After confirming that this extract was completely dissolved, make up to 1000 ml with purified water.
The mixture was stoppered and thoroughly stirred again to obtain an aqueous solution (containing 40 g / l of the present extract).

Formulation Example 1 (Preparation of Cream) Ingredient Group A: Mixture of polyoxyethylene (6) stearate, POE (20) cetyl ether, glyceryl stearate, and POE (20) stearyl ether 00 (wt%) hydrogenated castor oil 2.00 octyldodecyl myristate 5.00 polydecene 5.00 silicone oil (100cs) 2.00 Component group B: 2.00 mixture of dimethylpolysiloxane and dimethylpolysiloxanol Extract 4.00 Preservatives and fragrances qs Ingredients of Group A are heated to 75 ° C and stirred.
Purified water (100% by weight) heated to 75 ° C was added. The solution was cooled to about 45 ° C. and the silicone oil and the extract were added. After cooling completely, all other components were added, and the whole was thoroughly kneaded to obtain a cream.

Test Example 1 (Sensory evaluation) Ten healthy volunteer subjects were tested . The left and right portions of the subject's forehead were used as test sites, and the aqueous liquid (external test preparation) obtained in Formulation Example 1 was dropped on the left side, and a BSA-containing (3 wt%) aqueous solution (control external preparation) was dropped on the right side Thereafter, each was spread to be uniform at 5 cm ² . Next, when each subject evaluated the degree of tightening of the skin on the forehead to which both external preparations were applied, all the subjects that the aqueous liquid preparation of Formulation Example 1 had better skin tightening conditions than the aqueous solution containing BSA. Stated.

Test Example 2 (Observation by Scanning Electron Microscope) Formulation examples of 20 healthy volunteer subjects
The effect of the cream obtained in Example 2 was examined. First, skin replicas were made for two sites on both corners of the eyes. Then, apply the formulation to the replica collection site in the right eye corner
2 cream (external preparation) 14mg (1cm ² ) Apply,
Similarly, 14 mg (1 cm ² ) of a cream (external control) containing the same amount of BSA in place of the cream extract of Formulation Example 2 at the replica collection site at the left eye corner Applied. Application 6
0 minutes later, a skin replica of the above-described replica collection site is created again, and the obtained skin replica is scanned with a scanning electron microscope (HITACHI S800, manufactured by Hitachi, Ltd., connected to an imaging system, manufactured by Mamiya Kokki Co., Ltd.). Observed and photographed at 210x to analyze the surface of the skin replica. As a result, the cream of the present invention has a reduced wrinkle depth compared to before the application,
Compared to the BSA-containing cream, it was found that the skin had a smoother appearance.

Test Example 3 ( Determination of density of micro relief)
A) Skin replicas were prepared for two healthy female volunteers aged 45 to 50 years old at two sites in the outer corners of both eyes. After that, apply the aqueous liquid (external preparation for test) over 1 cm ² to the replica collection site
Apply 8 μl, and apply BSA to the replica collection site
(4% by weight) -containing aqueous solution (control external preparation) was applied in the same manner as above. After 60 minutes from the application, a skin replica of the above-mentioned replica collection site was again prepared. The obtained skin replica was imaged using a confocal microscope, and the image was subjected to three-dimensional analysis, thereby calculating the skin microreflex reduction rate by the following equation. (Number of micro wrinkles / total number of wrinkles) × 100 As a result, the density of the microreflex on the skin was reduced by 8% when the external preparation was applied, compared to before the application, When the control external preparation was applied, the reduction rate was 5% compared to before the application. This revealed that the extract had an effect of smoothing the skin, and that the extract had an effect equal to or higher than that of BSA in reducing the microrelief of the skin.

[0018]

The external preparation for skin according to the present invention tightens the skin, reduces the depth of wrinkles in the skin, and further smoothes the skin by reducing the micro relief of the skin. These effects are equal to or greater than those of an aqueous solution or cream containing BSA.

Claims (3)

[Claims] Is a method according to claim 1] leguminous plant white lupine (Lupi
nus albus L.) and an extract of Thymus vulgaris L., which is a Labiatae plant, as the main active ingredients,
External preparation for skin. 2. The external preparation for skin according to claim 1, wherein the dosage form is cream, ointment, emulsion, gel, film or liquid. 3. The content of both extracts is 0.1-50.0% by weight
3. The external preparation for skin according to claim 1 or 2, wherein: