CN115778837A - Synergistic skin-brightening repair freeze-dried powder and preparation method and application thereof - Google Patents
Synergistic skin-brightening repair freeze-dried powder and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a synergistic skin brightening and repairing freeze-dried powder which is prepared by freeze-drying the following solutions in parts by weight: an oxidoreductase: 1 to 5 percent; ganoderma lucidum extract: 1 to 3 percent; fibronectin: 2 to 5 percent; excipient: 3 to 5 percent; humectant: 0.02 to 0.04 percent; a soothing agent: 1 to 3 percent; pH buffer: 0.3 to 0.4 percent; the balance of water; wherein the amino acid sequence of the oxidoreductase is shown as SEQ ID NO. 1. The invention also discloses a preparation method and application of the synergistic skin-brightening repair freeze-dried powder. The oxidoreductase and the ganoderma lucidum extract in the formula of the skin-brightening and repairing freeze-dried powder have an obvious synergistic effect, so that the overall repairing effect of the freeze-dried powder is improved. The oxidoreductase in the formula is derived from rhodotorula benthica and can be obtained by using genetic engineering and fermentation technology, so that the biological safety is good.
Description
Technical Field
The invention belongs to the technical field of beauty and skin care, and particularly relates to a synergistic skin-brightening and repairing freeze-dried powder as well as a preparation method and application thereof.
Background
China is beautiful with white from ancient times and gives a sense of skin moistening and brightening. The reason for the dark skin and uneven skin color is that melanin is accumulated too much and distributed unevenly. Melanin overproduction is caused by many reasons, endocrine disorders due to stress, insufficient sleep, skin irritation due to air pollution, or ultraviolet exposure to sunlight. When melanin is transferred to the epidermis, the melanin is uniformly distributed to deepen the skin color of the whole body and make the skin color dark; uneven distribution causes a difference in skin color tone in a region, spots, and the like.
In recent years, biotechnology has been advanced in the field of beauty and skin care, and many biomaterials are continuously applied to skin care products and are accepted by wide consumers.
Disclosure of Invention
The inventor finds that one oxidoreductase naturally existing in Saccharomyces cerevisiae (Saccharomyces cerevisiae) can act on a plurality of reaction paths for melanin synthesis in a long-term research and development process, can effectively solve the skin problems, and has a very wide application prospect in the field of skin care. Thanks to the development of biotechnology, we can obtain the oxidoreductase by using genetic engineering and fermentation technology and develop and utilize it in skin care products.
Therefore, the first purpose of the invention is to provide a synergistic skin-brightening repair freeze-dried powder which can be used for homogenizing and brightening skin color and providing a good repairing effect on damaged skin.
A synergistic skin-brightening repair freeze-dried powder is obtained by freeze-drying the following solutions in parts by weight:
an oxidoreductase: 1 to 5 percent;
ganoderma lucidum extract: 1 to 3 percent;
fibronectin: 2 to 5 percent;
excipient: 3 to 5 percent;
humectant: 0.02 to 0.04 percent;
a soothing agent: 1 to 3 percent;
pH buffer: 0.3 to 0.4 percent;
the balance of water;
wherein the amino acid sequence of the oxidoreductase is shown in SEQ ID NO. 1.
According to a preferred embodiment of the invention, the excipient is one or more of mannitol, trehalose, glycine.
According to a preferred embodiment of the invention, the humectant is sodium hyaluronate.
According to a preferred embodiment of the invention, the soothing agent is peach gum extract.
According to a preferred embodiment of the invention, the pH buffer is sodium citrate.
In a second aspect of the present invention, a method for preparing the above synergistic skin-lightening repair lyophilized powder is provided, which comprises the following steps:
s1: heating a proper amount of deionized water in a container to 80-85 ℃, adding an excipient and a humectant, stirring until the excipient and the humectant are dissolved and dispersed uniformly, then continuing to add a soothing agent, and stirring the solution uniformly;
s2: adding a pH buffering agent, stirring until the pH buffering agent is dissolved and dispersed uniformly, and then adjusting the pH value of the solution to 6.0 by using citric acid;
s3: cooling the solution to a temperature of less than or equal to 45 ℃, then sequentially adding an active component oxidoreductase solution, a ganoderma lucidum extract solution and a fibronectin solution, and stirring to dissolve and uniformly disperse the active component oxidoreductase solution, the ganoderma lucidum extract solution and the fibronectin solution;
s4: adding deionized water to make the total solution weight be 1000g;
s5: and subpackaging the solution, and freeze-drying to obtain the freeze-dried powder.
According to a preferred embodiment of the invention, the oxidoreductase enzyme described in S3 is a 1mg/mL solution of oxidoreductase.
According to a preferred embodiment of the present invention, the ganoderma lucidum extract in S3 is a 20% ganoderma lucidum extract solution.
According to a preferred embodiment of the invention, the fibronectin solution described in S3 is a 500ppm fibronectin solution.
In a third aspect of the invention, the synergistic skin brightening repair freeze-dried powder is provided for preparing a skin brightening repair cosmetic or a skin care product.
The invention has the following beneficial effects:
1. the oxidoreductase and the ganoderma lucidum extract in the formula of the skin brightening and repairing freeze-dried powder have an obvious synergistic interaction effect, so that the overall skin brightening effect of the freeze-dried powder is improved.
2. The oxidoreductase and fibronectin in the formula also have an obvious synergistic effect, and the overall repair effect of the freeze-dried powder can be improved.
3. The oxidoreductase in the formula is derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae), can be obtained by using genetic engineering and fermentation technology, and has good biological safety.
Detailed Description
The technical solution of the present invention is clearly and completely described below with specific examples. It is to be understood that the described embodiments are only a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the invention without making creative efforts, fall within the scope of the invention.
The synergistic skin-brightening repair freeze-dried powder takes oxidoreductase, ganoderma lucidum extract and fibronectin as active components, and is obtained by freeze-drying the following solutions in parts by weight:
an oxidoreductase: 1 to 5 percent;
ganoderma lucidum extract: 1 to 3 percent;
fibronectin: 2 to 5 percent;
excipient: 3 to 5 percent;
humectant: 0.02 to 0.04 percent;
a soothing agent: 1 to 3 percent;
pH buffer: 0.3 to 0.4 percent;
the balance being water.
Sources of components used in the following examples:
the oxidoreductase is derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae), and its amino acid sequence is shown in SEQ ID NO. 1.
Ganoderma lucidum extract and fibronectin are commercially available from Meinjian (Shenzhen) Biotech, inc.
The other components are conventional commercial products.
Example 1 preparation of a synergistic skin lightening and repairing lyophilized powder
The synergistic skin lightening repair freeze-dried powder is prepared according to the formula shown in table 1 by the following steps:
s1: heating 700g of deionized water in a beaker to 80-85 ℃, adding an excipient and a humectant, stirring until the excipient and the humectant are dissolved and dispersed uniformly, then continuing to add a soothing agent, and stirring the solution uniformly;
s2: adding a pH buffering agent, stirring until the pH buffering agent is dissolved and dispersed uniformly, and then gradually dripping 1% citric acid to adjust the pH value of the solution to 6.0;
s3: cooling the prepared components in ice water to a temperature of less than or equal to 45 ℃, then sequentially adding 1mg/mL oxidoreductase solution, 20wt% ganoderma lucidum extract solution and 500ppm fibronectin solution, stirring to dissolve and uniformly disperse the components;
s4: adding a proper amount of normal-temperature deionized water to make the total weight of the material body be 1000g;
s5: subpackaging the material body into 5ml penicillin bottles, wherein each penicillin bottle contains 2g of the material body; and transferring the penicillin bottle to a freeze dryer for freeze drying after subpackaging is finished, and obtaining freeze-dried powder after the freeze drying is finished.
As shown in Table 1, A-F6 lyophilized powders with different ratios were prepared.
TABLE 1 preparation of skin lightening and repairing lyophilized powder
Example 2 Freeze-dried powder comprehensive skin lightening test
Selecting persons whose facial complexion is dark and uneven with naked eyes, recruiting 30 volunteers with age of 20-40 years, randomly dividing into three groups of 1-3, and 10 persons in each group.
The instrument comprises: alice Ci64 (usa), VISIA (usa).
The test method comprises the following steps: 1-3 groups of volunteers respectively try the freeze-dried powder A, B, C twice a day in the morning and at night for two weeks. Before and after trial, each volunteer was measured by Ci64 to calculate the value of the Individual Type Angle (ITA), and simultaneously, the volunteers were photographed by VISIA and the scores of the facial items were recorded.
Data processing: before trial, ITA degree data of volunteers are recorded as ITA1, and the average of each group is calculated
Recording ITA degree data after trial as ITA2, and averaging the average of each group
And finally, operating each group of data:
the results are shown in Table 2.
TABLE 2
Higher ITA ° values represent lighter overall skin color, appearing whiter and brighter;
then it represents the average initial state of the present set of skin tones,
representing the average state of the complexion of the group after trial use of the freeze-dried powder;
the larger the skin color of the group member, the more obvious the skin color changes from dark to light.
As can be seen from the data in Table 1The skin color of 1, 2 and 3 groups of volunteers is lightened, wherein the change of the 3 rd group is the largest, the skin color is lightened most obviously, the change of the 1 st group is the smallest, and the skin color lightening degree is the lowest. Group 3 of
The data is less different from the data in group 2, and the proportion scheme of the freeze-dried powder B is considered comprehensively and is preferred.
Example 3 synergistic experiment of oxidoreductase and Ganoderma lucidum extract
Selecting human with dark skin and uneven skin which can be identified by naked eyes, recruiting 20 volunteers, the age of 20-40 years, randomly dividing into two groups of 4 and 5, and 10 people in each group.
The instrument comprises the following steps: alice Ci64 (usa), VISIA (usa).
The test method comprises the following steps: two groups of volunteers respectively tried the lyophilized powder E, F twice a day in the morning and evening for two weeks. Before and after trial, each volunteer was measured by Ci64 to calculate the value of the Individual Type Angle (ITA), and simultaneously, the volunteers were photographed by VISIA and the scores of the facial items were recorded.
Data processing:
1) Before trial, ITA degree data of volunteers are recorded as ITA1, and the average of each group is calculated
Recording ITA degree data after trial as ITA2, and averaging the average of each group
And finally, operating each group of data:
and data of volunteers of example 1, group 2 using lyophilized powder B and group 3 using lyophilized powder C were compared, and the results are shown in table 3.
2) Two scoring data of ultraviolet Spots (UV Spots) and Brown Spots (Brown Spots) obtained from VISIA by taking 4 and 5 groups of volunteers from face are respectively recorded as UVS and BS, wherein the scores before trial are UVS1 and BS1, and the scores after trial are UVS2 and BS2; averaging the data of each group to obtain
And finally, operating each group of data:
the data of UV spots and brown spots photographed on the face of volunteers of example 1, group 2 using lyophilized powder B and group 3 using lyophilized powder C were introduced and processed as above, and the results are shown in Table 4.
TABLE 3
Lyophilized powder E was identical to lyophilized powder C except that no Ganoderma lucidum extract was added to the components, but as can be seen from the data in Table 3, group 4
Has obvious difference with group 3, even lower than group 2, but the addition amount of other components is not as high as that of the freeze-dried powder E except that the ganoderma lucidum extract is added into the freeze-dried powder B. Whereas the data for group 5 using lyophilized powder F was worse, much lower than for groups 2 and 3.
TABLE 4
One of the VISIA test items for facial photography is uv spots, which are used to evaluate the formation of melanin accumulated under the skin surface after the face is damaged by sunlight and uv light, and brown spots, which are used to test the excess melanin produced by melanocytes in the bottom layer of the epidermis. Both are important factors affecting the uniformity of skin color and the overall shade of skin color, and the same higher score indicates less problems.
As can be seen from the results in Table 4, the data of group 4
There was a clear difference from group 3, even lower than group 2. The data of group 5 is much lower than those of groups 2 and 3. It is easy to see that when only one of the oxidoreductase and the ganoderma lucidum extract exists, the effect of the freeze-dried powder is far inferior to the effect of simultaneously adding the oxidoreductase and the ganoderma lucidum extract, which shows that the oxidoreductase and the ganoderma lucidum extract can synergistically enhance the skin lightening effect of the freeze-dried powder and can better and uniformly color the skin.
Example 4 comprehensive repair experiment of lyophilized powder
30 volunteers aged 20-40 years who were susceptible to facial skin were recruited into groups of 6-8 individuals each with 10 individuals.
The instrument comprises the following steps: VISIA (usa).
The test method comprises the following steps: lyophilized powder A, B, C was tried twice a day in the morning and at the evening for two weeks on 6-8 groups of volunteers. And simultaneously take a photograph of the face of the volunteer with VISIA and record the facial item scores.
Data processing: obtaining the RED distinguishing value of face photographing of the volunteer from VISIA, recording the RED distinguishing value before the volunteer trial as RED1, and averaging the average of each group
Recording the RED differential value after the trial is finished as RED2, and averaging the average value of each group
And finally, operating each group of data:
the results are shown in Table 5.
TABLE 5
The red area is one of the items analyzed after VISIA facial photographs were taken, which can reflect many skin problems such as skin inflammation, skin sensitivity, spider veins, etc., and can be used to assess the overall health of the skin. A higher red score indicates less skin problems and healthier skin.
As can be seen from the results in Table 5, the scores of the red areas of the volunteers in the groups 6 to 8 were all significantly improved, with the largest change in the group 8 and the smallest change in the group 6. Group 8 of
Compared with the group 7, the difference is smaller, and the proportion scheme of the freeze-dried powder B is considered comprehensively and is preferred.
Example 5 synergistic interaction of oxidoreductase with fibronectin
Persons with sensitive facial skin recruit 20 volunteers aged 20-40 years and randomly divided into two groups of 9 and 10, each group containing 10 persons.
The instrument comprises the following steps: VISIA (usa).
The test method comprises the following steps: the lyophilized powder D, F was tried twice a day in the morning and at the evening for two weeks on 9 and 10 groups of volunteers. And simultaneously take a photograph of the face of the volunteer with VISIA and record the facial item scores.
Data processing: obtaining the RED distinguishing value of face photographing of the volunteer from VISIA, recording the RED distinguishing value before the volunteer trial as RED1, and averaging the average of each group
Marking the RED distinguishing value after trial as RED2, and averaging the average value of each group
And finally, operating each group of data:
and the data of the 7 th and 8 th groups in example 4 were imported for comparison, and the results are shown in Table 6.
TABLE 6
As can be seen from the results in Table 6, groups 9 and 10 used lyophilized powder D, F, respectively
Are smaller than groups 6 and 7. It is easy to see that when only one of the oxidoreductase and fibronectin is added, the repairing effect of the lyophilized powder is far less than that when both are added, and particularly, the effect is not obvious when only the oxidoreductase is added. Therefore, the oxidoreductase and the fibronectin have obvious synergy, and when the oxidoreductase and the fibronectin are added together, the overall repair effect of the freeze-dried powder can be improved.
While the foregoing embodiments show and describe the basic principles, principal features and advantages of the invention, it will be understood by those skilled in the art that the invention is not limited thereto, but is described in the foregoing embodiments and description only to illustrate the principles of the invention and that various changes and modifications may be made therein without departing from the spirit and scope of the invention and these changes and modifications are also within the scope of the invention.
Claims (10)
1. The synergistic skin-brightening repair freeze-dried powder is characterized in that the skin-brightening repair freeze-dried powder is obtained by freeze-drying the following solutions in parts by weight:
an oxidoreductase: 1 to 5 percent;
ganoderma lucidum extract: 1 to 3 percent;
fibronectin: 2 to 5 percent;
excipient: 3 to 5 percent;
humectant: 0.02 to 0.04 percent;
a soothing agent: 1 to 3 percent;
pH buffer: 0.3 to 0.4 percent;
the balance of water;
wherein the amino acid sequence of the oxidoreductase is shown as SEQ ID NO. 1.
2. The synergistic skin lightening freeze-dried powder according to claim 1 wherein the excipient is one or more of mannitol, trehalose, glycine.
3. The synergistic skin lightening freeze-dried powder of claim 1 wherein the humectant is sodium hyaluronate.
4. The synergistic skin lightening freeze-dried powder of claim 1 wherein the soothing agent is peach gum extract.
5. The synergistic skin lightening freeze-dried powder of claim 1 wherein the pH buffer is sodium citrate.
6. A method of preparing a synergistic skin lightening freeze-dried powder according to any of claims 1 to 5 characterized in that it comprises the steps of:
s1: heating a proper amount of deionized water in a container to 80-85 ℃, adding an excipient and a humectant, stirring until the excipient and the humectant are dissolved and dispersed uniformly, then continuing to add a soothing agent, and stirring the solution uniformly;
s2: adding a pH buffering agent, stirring until the pH buffering agent is dissolved and dispersed uniformly, and then adjusting the pH value of the solution to 6.0 by using citric acid;
s3: cooling the solution to a temperature of less than or equal to 45 ℃, then sequentially adding an active component oxidoreductase solution, a ganoderma lucidum extract solution and a fibronectin solution, and stirring to dissolve and uniformly disperse the active component oxidoreductase solution, the ganoderma lucidum extract solution and the fibronectin solution;
s4: adding deionized water to make the total solution weight be 1000g;
s5: and subpackaging the solution, and freeze-drying to obtain the freeze-dried powder.
7. The method of claim 6, wherein the oxidoreductase in S3 is a 1mg/ml oxidoreductase solution.
8. The method of claim 6, wherein the Ganoderma lucidum extract in S3 is a 20% Ganoderma lucidum extract solution.
9. The method of claim 6, wherein the fibronectin solution in S3 is a 500ppm fibronectin solution.
10. Use of a synergistic skin lightening lyophilized powder according to any of claims 1 to 5 for the preparation of a skin lightening or repairing cosmetic or skin care product.
Priority Applications (1)
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