CN110664643A - Polypeptide essence with multiple-effect whitening effect and preparation method thereof - Google Patents

Abstract

The invention discloses polypeptide essence with multiple-effect whitening effect and a preparation method thereof. Meanwhile, the high-concentration functional substances are adopted to achieve the efficient whitening effect, and the aloe extract, the dendrobium stem extract, the fructus lycii extract, the echinacea purpurea extract and other plant extracts are compounded to form a stability maintaining system, so that the skin is prevented from being damaged by the high-concentration whitening functional substances, and the skin is efficiently whitened in a safe and stable environment.

Description

Polypeptide essence with multiple-effect whitening effect and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to polypeptide essence with a multi-effect whitening effect and a preparation method thereof.

Background

Melanocytes, one of the important constituent cells of the skin, are dendritic processes through which melanin formed within the cell is transported into the cells of the stratum corneum. It is prepared by the following steps of 1: the 36 ratio constitutes one epidermal unit with keratinocytes, about 1500 per square millimeter. Melanin has effects of protecting skin against ultraviolet, tyrosine is used as main raw material for preparing melanin, tyrosinase is the main rate-limiting enzyme for converting tyrosine into melanin, and catalytic efficiency is 6X10²²The instant black coating effect can be achieved; in addition, melanin is transferred to keratinocytes and transferred to surface cells layer by layer to form apparent skin color; meanwhile, the horny layer of epidermis is too thick and does not appear glossy, which is one of the reasons why the skin is not fair enough.

In the Chinese society, which is believed to be 'white covering ugly', the health whitening problem of women is always a beauty problem which is concerned about. Ancient palace beauty treatment uses mercury compounds to whiten the skin, and after 6 weeks of use, the whitening effect is obvious. With the advance of science and technology, people deepen the understanding of the extreme toxicity of mercury to human bodies and have been banned for a long time. At present, one of the most effective skin whitening actives is hydroquinone, i.e., hydroquinone, which acts to inhibit melanin formation by inhibiting the activity of tyrosinase, but hydroquinone, which has a small molecular weight, destroys melanocytes, and may cause skin irritation and contact dermatitis. In addition, hydroquinone is also classified as a potential carcinogenic substance. Therefore, hydroquinone is subject to strict regulations in most countries of the world and is prohibited from being used as a cosmetic ingredient.

At present, in order to make a short-term profit, some illegal merchants add glucocorticoid to cosmetics such as facial masks. The whitening and firming effect can be generated in a short time, but the long-term use of the whitening and firming agent has very serious consequences on the skin of a human. However, since the product design of whitening mind and urgent work is urgently required for a long time, many whitening skin care products are in serious misdirection, pursue rapid whitening, depart from the track of natural growth rule and scientific rule, and the whitening products with large toxic and side effects mostly cause skin dependence and fall into a vicious circle state to damage the skin health. In recent years, therefore, safe whitening skin care products are gradually recognized and accepted by the most distant women. At present, whitening products on the market are continuously emerging, and most of the products adopt nicotinamide for whitening, which can quickly obtain whitening effect by blocking the transfer of melanin to the surface layer, but can quickly rebound once being stopped, can not systematically inhibit the growth chain of the melanin, and are difficult to realize the effects of real whitening, spot lightening, skin color balancing and skin luster recovery. Therefore, it is imperative to find an efficient and safe whitening regimen.

Disclosure of Invention

In order to solve the problems, the invention provides a polypeptide essence with multiple-effect whitening effect and a preparation method thereof, and the invention aims to provide the polypeptide essence with the functions of point-line-surface and multi-direction whitening, wherein the polypeptide essence is synergistic by using multiple whitening mechanisms and has high whitening efficiency.

The invention is realized by the following technical scheme:

a polypeptide essence with multiple-effect whitening effect is composed of the following raw materials in parts by weight:

the preferable polypeptide essence with the multiple-effect whitening effect is prepared from the following raw materials in parts by weight: 68.1 parts of aloe extract, 1 part of reed extract, 1 part of licorice root extract, 1 part of angelica root extract, 1 part of salvia miltiorrhiza root extract, 1 part of dogwood fruit extract, 1 part of wolfberry root extract, 0.7 part of radix ophiopogonis extract, 1 part of rumex acetosa extract, 0.6 part of twisted cactus stem extract, 0.02 part of sophora flavescens ait root extract, 3 parts of 1, 2-pentanediol, nonapeptide-16 parts, 2 parts of carnosine, 0.3 part of ascorbic acid, 3 parts of nicotinamide, 3 parts of osmotic humectant, 2 parts of linlan lotion, 3 parts of rhamnose silanol, 0.8 part of trehalose, 1 part of dihydroavenoyl anthranilic acid, 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer and 0.1 part of PEG-40 hydrogenated castor oil.

Further, the rhamnosilanol is a mixture of 0.3 parts by weight of silanetriol, 2.35 parts by weight of rhamnose and 20 parts by weight of methylpropanediol.

Further, the permeable humectant is a mixture of erythritol and creatinine HCl, and the mass ratio of the erythritol to the creatinine HCl is 100: 1.

The essence also comprises 0.1-1 weight part of a mixture of artemisia capillaris flower extract, clove flower extract and glyceryl caprylate; the mass ratio of the artemisia capillaris flower extract to the clove flower extract to the glyceryl caprylate is 20-25: 10-15: 3-5.

Further, the preparation method of the aloe extract comprises the following steps:

s1, selecting ripe aloe, cleaning, and pulping in a sealed environment;

s2, adding 5-8 times of 85-98% 1, 3-propylene glycol solution as solvent into the aloe pulp, performing ultrasonic treatment at 35-45 deg.C for 20min, and filtering to obtain the first filtrate;

s3, adding 10 parts by weight of distilled water into the obtained filter residue, adding degrading enzyme, stirring uniformly, standing for 30min, and filtering to obtain a second filtrate;

s4, mixing the first filtrate and the second filtrate, and mixing to obtain the aloe extract.

Further, the degrading enzyme is a mixture of 0.15-0.2 weight part of cellulase, 0.023-0.028 weight part of pectinase and 0.015-0.018 weight part of amylase.

The invention also discloses a preparation method of the polypeptide essence with the multi-effect whitening effect, which comprises the following steps:

a1, mixing Aloe extract, Phragmites communis extract and Glycyrrhrizae radix extract;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, a penetrating humectant, a linlan lotion, rhamnose silanol, trehalose, a radix ophiopogonis extract, a twisted cactus stem extract, a sophora flavescens root extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing stably and discharging.

The invention has the beneficial effects that:

1. the invention takes the polypeptide as the core and compounds various functional raw materials, inhibits the activity of the tyrosinase, reduces the synthesis of melanin, blocks the transfer of the generated melanin to surface cells, reduces the generated melanin, improves skin microcirculation and the like, and performs whitening from point and line surfaces in multiple directions under the synergistic action of various whitening mechanisms, thereby enabling the skin to be transparent and bright from inside to outside. Meanwhile, the high-concentration functional substances are adopted to achieve the efficient whitening effect, and the aloe extract, the dendrobium stem extract, the fructus lycii extract, the echinacea purpurea extract and other plant extracts are compounded to form a stability maintaining system, so that the skin is prevented from being damaged by the high-concentration whitening functional substances, and the skin is efficiently whitened in a safe and stable environment.

2. The nonapeptide-1 of the invention blocks the activation of tyrosinase and reduces the generation of melanin by competitively antagonizing the combination of natural ligand melanocyte stimulating hormone alpha-MSH and receptor MCR-1 on melanocyte; the small molecular polypeptide is used as an endogenous active substance, is easy to absorb, and has high affinity and high efficiency with skin. Meanwhile, the addition of the carnosine prevents peroxidation in the skin, reduces the oxidative damage of the skin, promotes the synthesis of collagen, protects skin cells from DNA damage caused by ultraviolet irradiation, and prevents the damage of infrared rays. The skin wrinkle and dullness are improved in many aspects, the skin firmness and glossiness are improved, and the young and healthy skin is recovered. The aloe extract, the reed extract, the licorice root extract, the angelica root extract and other extracts are carefully matched, so that the aloe extract, the reed extract, the licorice root extract, the angelica root extract and other extracts can effectively resist and eliminate free radicals, inhibit hyaluronidase and tyrosinase, and have good antioxidant activity; the skin care product can also effectively improve the dull state of the skin caused by oxidative damage, effectively improve the microcirculation blood flow of the skin, improve the microcirculation, promote the absorption of nonapeptide-1 and nicotinamide, and further improve the effects of whitening and brightening, thereby comprehensively improving the skin state. Has effects of mildly soothing skin; can also promote the synthesis of aquaporins, has the effect of deeply moisturizing, and makes the skin emit healthy and natural luster, and the skin be transparent and glossy from inside to outside.

3. The aloe extract is extracted by a method of synergy of propylene glycol hydrosolvent, ultrasonic waves and enzymolysis, so that the extraction efficiency is improved, high-temperature extraction is avoided, the phenomenon of inactivation of the extract at high temperature is reduced, meanwhile, pectin is decomposed by the enzymolysis, the viscosity is reduced, the juice yield is improved, the skin feel is improved, and meanwhile, cellulose is decomposed into oligosaccharide and polysaccharide, the molecular weight is reduced, and the absorption by the skin is facilitated. The aloe extract is rich in polysaccharide, barbaloin, amino acid, various organic acids, trace elements and the like, has the effects of relieving, repairing and improving reddened skin and enhancing cell activity besides the basic moisturizing and moisturizing effect, and can play a synergistic effect with polypeptide and nicotinamide to enhance the whitening effect. The propylene glycol is used as an extraction solvent II, so that ethanol is avoided, the skin is prevented from being stimulated by a large amount of residual ethanol, the dissolution of effective components can be increased by the propylene glycol, and the skin care effects such as moisture retention and the like can be achieved.

4. The invention plays a role in balancing whitening and brightening through multi-azimuth combination of point line surfaces, and points are as follows: ascorbic acid reduces already produced melanin, line: linlan lotion smooth and fine lines, make skin transparent and bright, face: the extracts of nonapeptide-1, radix Angelicae sinensis, and Glycyrrhrizae radix extract have effects of inhibiting activity of tyrosinase and improving skin color.

5. The cold preparation method is adopted to prepare the essence, so that the inactivation of active substances caused by improper temperature is completely avoided, meanwhile, no production water is added in the whole preparation process, a large amount of aloe extract is used for substitution, more active substances are injected into the formula, the moisture is preserved, the water is supplemented, and the skin care effect is better.

Drawings

FIG. 1 is a schematic diagram of the evaluation of apparent chromaticity of a blank control group;

FIG. 2 is a schematic diagram showing the evaluation of apparent color of kojic acid solution

FIG. 3 is a schematic diagram showing the evaluation of the apparent chromaticity of the essence prepared in example 3;

FIG. 4 is a graph showing the evaluation of the apparent brightness of the essence prepared in example 3;

fig. 5 is a graph illustrating the melanin content of the serum prepared in example 3.

Detailed Description

In order to better understand the present invention, the following examples are further described, which are only used to explain the present invention and do not limit the present invention.

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products. Purchase factories offering commercially available products

Silanetriol, rhamnose and methylpropanediol are from acerola, france; the extract of Rumex occidentalis is from Lucas Meier, USA; ascorbic acid is from Shanghai national drug group, Inc.; the osmotic moisturizer, erythritol, and carnosine HCl are from france semama; the radix Sophorae Flavescentis extract is obtained from Sienna Lingfeng Biotechnology GmbH; the Linlan Runlu, the stem extract of Opuntia ficus-indica, the root extract of Glycyrrhiza uralensis Fisch, the root extract of Angelica sinensis, the root extract of Salvia miltiorrhiza Bunge, the fruit extract of Cornus officinalis Sieb, the root extract of Lycium barbarum, the root extract of Ophiopogon japonicus, the stem extract of Opuntia ficus-indica and the root extract of Sophora flavescens are from vast Sen Biotech limited of Beijing.

Example 1

The preparation method of the aloe extract comprises the following steps:

a1, selecting ripe aloe, cleaning, and pulping in a sealed environment;

a2, adding 5 times of 85% 1, 3-propylene glycol solution as solvent into the aloe slurry, performing ultrasonic treatment at 35 deg.C for 20min, and filtering to obtain the first filtrate;

a3, adding 10 parts by weight of distilled water into the obtained filter residue, adding degrading enzyme, stirring uniformly, standing for 30min, and filtering to obtain a second filtrate;

a4, mixing the first and second filtrates, and mixing to obtain Aloe extract.

Best mode for carrying out the invention

The preparation method of aloe extract comprises:

a1, selecting ripe aloe, cleaning, and pulping in a sealed environment;

a2, adding 8 times of 98% 1, 3-propylene glycol solution as solvent into the aloe slurry, performing ultrasonic treatment at 45 deg.C for 20min, and filtering to obtain the first filtrate;

a3, adding 10 parts by weight of distilled water into the obtained filter residue, adding degrading enzyme, stirring uniformly, standing for 30min, and filtering to obtain a second filtrate;

a4, mixing the first and second filtrates, and mixing to obtain Aloe extract.

Best mode for carrying out the invention

A polypeptide essence with multiple-effect whitening effect is composed of the following raw materials in parts by weight: 50 parts of aloe extract; 0.5 part of reed extract; 0.5 part of licorice root extract; 0.5 part of angelica root extract; 0.5 part of salvia miltiorrhiza root extract; 0.5 part of dogwood fruit extract; 0.5 part of wolfberry root extract; 1 part of 1, 2-pentanediol; nonapeptide-18 parts; 2 parts of carnosine; 1 part of rumex acetosa extract; 0.5 part of ascorbic acid; 5 parts of nicotinamide; 3.5 parts of a penetrating humectant; 5 parts of rhamnose silanol; 10 parts of forest blue moistening lotion; 3 parts of rhamnose silanol; trehalose 0.3 parts, ophiopogon root extract 0.34 parts, prickly pear stem extract 0.35 parts, and flavescent sophora root extract 0.01 parts; 0.3 part of dihydrooat acyl anthranilic acid; 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer; 0.1 part of PEG-40 hydrogenated castor oil.

The preparation method comprises the following steps

A1, mixing Aloe extract, Phragmites communis extract and Glycyrrhrizae radix extract;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, then adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, nicotinamide, erythritol, sarcosine HCl, silanetriol, rhamnose, methyl propylene glycol, linlan dew, trehalose, a radix ophiopogonis extract, a prickly pear stem extract, a radix sophorae flavescentis extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing for stabilization, and discharging.

Example 4

A polypeptide essence with multiple-effect whitening effect is composed of the following raw materials in parts by weight: 68.1 parts of extract, 3 parts of 1, 2-pentanediol, 16 parts of nonapeptide, 2 parts of carnosine, 3 parts of nicotinamide and 2.5 parts of rumex acetosa extract; 0.3 part of ascorbic acid; 5 parts of nicotinamide; an osmotic humectant 3; 4 parts of rhamnose silanol; 2 parts of forest orchid hydrosol, 0.8 part of reed extract, 1 part of licorice root extract, 0.8 part of angelica root extract, 1 part of salvia miltiorrhiza root extract, 1 part of dogwood fruit extract, 0.5 part of boxthorn root extract, 1 part of trehalose, 1.2 parts of radix ophiopogonis extract, 0.88 part of prickly pear stem extract, 0.02 part of sophora flavescens ait root extract, 1 part of dihydroavenyl anthranilic acid, 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer and 0.1 part of PEG-40 hydrogenated castor oil.

The preparation method comprises the following steps

A1, mixing Aloe extract, Phragmites communis extract and Glycyrrhrizae radix extract;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, then adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, nicotinamide, erythritol, sarcosine HCl, silanetriol, rhamnose, methyl propylene glycol, linlan dew, trehalose, a radix ophiopogonis extract, a prickly pear stem extract, a radix sophorae flavescentis extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing for stabilization, and discharging.

Example 5

A polypeptide essence with multiple-effect whitening effect is composed of the following raw materials in parts by weight: 80 parts of aloe extract; 1 part of reed extract; 1 part of licorice root extract; 1 part of angelica root extract; 1 part of salvia miltiorrhiza root extract; 0.8 part of dogwood fruit extract; 1 part of wolfberry root extract; 5 parts of 1, 2-pentanediol; nonapeptide-18 parts; 2 parts of carnosine; 5 parts of nicotinamide; 1 part of rumex acetosa extract; 0.7 part of ascorbic acid; 5 parts of nicotinamide; 5 parts of a penetrating humectant; 5 parts of rhamnose silanol; 10 parts of forest blue moistening lotion; 1.5 parts of trehalose, 1.5 parts of radix ophiopogonis extract, 1.95 parts of twisted cactus stem extract and 0.05 part of sophora flavescens root extract; 2 parts of dihydrooat acyl anthranilic acid; 1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer; 1 part of PEG-40 hydrogenated castor oil.

The preparation method comprises the following steps

A1, mixing Aloe extract, Phragmites communis extract and Glycyrrhrizae radix extract;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, then adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, nicotinamide, erythritol, sarcosine HCl, silanetriol, rhamnose, methyl propylene glycol, linlan dew, trehalose, a radix ophiopogonis extract, a prickly pear stem extract, a radix sophorae flavescentis extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing for stabilization, and discharging.

Comparative example 1

The preparation method of aloe extract comprises:

1. selecting mature aloe vera, cleaning, and pulping in a sealed environment.

2. Adding 8 times of water solvent as solvent into the aloe slurry, performing ultrasonic treatment at 35 deg.C for 20min, and filtering to obtain the first filtrate.

4. Adding 10 times of distilled water into the obtained filter residue, uniformly stirring, standing for 30min, and filtering to obtain a second filtrate.

5. Mixing the first filtrate and the second filtrate, and mixing to obtain Aloe extract.

Comparative example 2

50 parts of water; 0.5 part of reed extract; 0.5 part of licorice root extract; 0.5 part of angelica root extract; 0.5 part of salvia miltiorrhiza root extract; 0.5 part of dogwood fruit extract; 0.5 part of wolfberry root extract; 1 part of 1, 2-pentanediol; nonapeptide-18 parts; 2 parts of carnosine; 1 part of rumex acetosa extract; 0.5 part of ascorbic acid; 5 parts of nicotinamide; 3.5 parts of a penetrating humectant; 5 parts of rhamnose silanol; 10 parts of forest blue moistening lotion; 3 parts of rhamnose silanol; trehalose 0.3 parts, ophiopogon root extract 0.34 parts, prickly pear stem extract 0.35 parts, and flavescent sophora root extract 0.01 parts; 0.3 part of dihydrooat acyl anthranilic acid; 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer; 0.1 part of PEG-40 hydrogenated castor oil.

The preparation method comprises the following steps

A1, mixing water and Glycyrrhrizae radix extract uniformly;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, then adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, nicotinamide, erythritol, sarcosine HCl, silanetriol, rhamnose, methyl propylene glycol, linlan dew, trehalose, a radix ophiopogonis extract, a prickly pear stem extract, a radix sophorae flavescentis extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing for stabilization, and discharging.

Comparative example 3

50 parts of aloe extract; 0.5 part of angelica root extract; 0.5 part of dogwood fruit extract; 0.5 part of wolfberry root extract; 1 part of 1, 2-pentanediol; nonapeptide-18 parts; 2 parts of carnosine; 8.5 parts of nicotinamide; 3.5 parts of a penetrating humectant; 5 parts of rhamnose silanol; 10 parts of forest blue moistening lotion; 3 parts of rhamnose silanol; trehalose 0.3 parts, ophiopogon root extract 0.34 parts, prickly pear stem extract 0.35 parts, and flavescent sophora root extract 0.01 parts; 0.3 part of dihydrooat acyl anthranilic acid; 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer; 0.1 part of PEG-40 hydrogenated castor oil.

The preparation method comprises the following steps

A1, mixing Aloe extract, radix Angelicae sinensis extract, Corni fructus extract and Lycii radix extract at room temperature;

a2, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil;

a3, adding the mixture of the step A1 and the step A2, mixing and stirring uniformly, then adding nonapeptide-1, carnosine, nicotinamide, erythritol, carnosine HCl, silanetriol, rhamnose, methyl propylene glycol, linlan emollient water, trehalose, an ophiopogon root extract, a twisted cactus stem extract, a sophora flavescens root extract, dihydroavenyl anthranilic acid and acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing and discharging after stabilization.

Results of the experiment

1. The aloe extracts prepared in comparative example 1 and examples 1-2 were subjected to skin feel test; the results are shown in Table 1

TABLE 1 Aloe extract skin feeling test Table

	Barbaloin content (mg/g)	Skin feel (trial for 20)
			Comparative example 1	186	Viscous (15 persons)
Example 1	205	Fresh and cool (19 persons)
			Example 2	203	Fresh and cool (19 persons)

The above table shows that the aloe extracts prepared in examples 1-2 are not sticky and give a refreshing skin feel.

2. The samples of example 3 and comparative example 3 were administered to 20 volunteers with similar skin conditions for 30 days, and used after cleansing in the morning and evening; the situation was recorded and the effect was followed after 15 days. (20 persons per group); the results are shown in Table 2.

Table 2 comparative table of skin condition after use of samples of example 3 and comparative example 3

As can be seen from the data in the table, the aloe extract has good relieving effect, and does not cause skin irritation and produce a reddening phenomenon. From the whitening data, the addition of the aloe extract also has a certain synergistic auxiliary effect on the whitening effect.

3. The samples of example 3 and comparative example 2 were administered to 20 volunteers with similar skin conditions for 30 days, and were used after cleansing in the morning and evening; the situation was recorded and the effect was followed after 15 days. (20 persons per group); the results are shown in Table 3.

Table 3 comparative table of skin condition after use of samples of example 3 and comparative example 2

As can be seen from the data in table 3, the total amount of the whitening agent used was 8.5 parts, but the whitening effect in example 3 was significantly better than that in comparative example 2. The combination of the whitening components of the invention has high whitening effect, and simultaneously reduces the irritation of the single whitening component nicotinamide.

4. Human body patch test

The essence prepared in example 3 of the present invention was tested by 35 volunteers according to the specifications of the skin patch test for human body described in the "standards for hygiene of cosmetics" (2007 edition), and the results are shown in the following table 4:

table 4 test record table for spot-sticking of human skin

As can be seen from the above table 4, the essence has no possibility of adverse reaction to human skin, which indicates that the formula of the essence is non-irritant and very safe.

5. And (3) evaluating the efficacy:

5.1 this efficacy evaluation entrusts third party testing company- -Guangdong Boxi Biotechnology Ltd to test

The experiment uses a 3D melanin skin model

The in-vitro whitening effect of different sample groups is evaluated by three dimensional indexes of an apparent picture of a model after administration, the brightness (L value) of the skin model, the melanin content and the like.

From the day of model shipment (defined as day 0), UVB irradiation treatment (50mJ/cm2) is carried out every day, samples to be tested are evenly coated on the surface of a melanin model after continuous stimulation is carried out for 3 days, the dosage volume is 10 mu L/time, 1 time is carried out every 2 days, and the operation is finished after continuous stimulation is carried out for 4 days and is ready for use.

After the treatment of the administration mode is finished, the models of different experimental groups are subjected to apparent photographing observation. The photographing is carried out in a studio with fixed light intensity. The specific photographing standard operation is as follows: (1) a camera mode: manual operation; setting photographing parameters: focal length 5.8mm, aperture F/8, aperture F22, shutter speed 1/80s, ISO 3200. (2) And (4) placing the melanin model in the center of the colorimetric card for photographing.

L-value measurements were performed on the models of the different experimental groups. The specific detection standard operation is as follows: (1) cutting off the model along the edge ring of the small chamber by using a surgical blade; (2) and placing the model on a flat and hard white plane, vertically aligning a detection hole of the color difference meter with the surface of the model according to the use instruction of the color difference meter, detecting, repeatedly reading for three times for each model, and recording data.

The model to be tested adopts 0.6mL of 0.25% pancreatin, and is kept stand and digested for 2 hours at 37 ℃; after finishing, repeatedly blowing and beating the tissues for about 1min by using an elbow suction pipe; after the blowing is finished, taking out the separated PC film by using an elbow tweezers; and (4) terminating: adding 0.6mL of PBS containing 10% serum, centrifuging at 2000r/min for 10min, and removing the supernatant; adding 1N NaOH (containing 10% DMSO) into the precipitate, blowing and beating for several times, reacting in 80 deg.C water bath for 30min to completely dissolve melanin particles, centrifuging at 1200r/min for 5min, collecting supernatant, adding into 96-well plate, adding 200 μ L per well, and detecting OD value at ELISA reader A405nm

Fig. 1 to 3 are the apparent chromatograms of the blank control group, kojic acid and the essence of example 3, respectively, from which it can be seen that the apparent chromaticity of the essence is lighter than that of kojic acid, indicating that the whitening effect of the essence is better than that of kojic acid.

Fig. 4 is an evaluation of the apparent chromaticity of the control group with or without UVB irradiation and the treated serum, and it can be seen that the evaluation of the apparent chromaticity after the treated serum is almost higher than that of the treated serum which is irradiated only by UVB and is close to that of the treated serum which is not irradiated by UVB, which indicates that the serum has a very good whitening effect and can resist the pigmentation caused by UVB.

Fig. 5 shows the presence or absence of UVB irradiation, and the melanin content after kojic acid and serum treatment under UVB irradiation, and it can be seen that the melanin content after serum treatment is lower than that of kojic acid treatment, indicating that the whitening effect of the serum is better than that of kojic acid. The content of melanin after the treatment of the essence is even lower than that of melanin which is not subjected to UVB irradiation, and further shows that the essence has a good whitening effect.

5.2 type test-attorney Spectrum Nile Limited test, the results are as follows 5:

TABLE 5 type test result recording sheet

As can be seen from table 5 above, the essence of the present application meets the standards of skin care products.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (8)

1. The polypeptide essence with the multiple-effect whitening effect is characterized by comprising the following raw materials in parts by weight:

50-80 parts of aloe extract;

0.5-1 part of reed extract;

0.5-1 part of licorice root extract;

0.5-1 part of angelica root extract;

0.5-1 part of salvia miltiorrhiza root extract;

0.5-1 part of dogwood fruit extract;

0.5-1 part of wolfberry root extract;

0.34-1.5 parts of radix ophiopogonis extract;

0.35-1.95% of the extract of the stem of the prickly pear;

0.01-0.05 part of sophora flavescens root extract;

1-3 parts of a rumex acetosa extract;

1-5 parts of 1, 2-pentanediol;

nonapeptide-12-8 shares;

0.1-2 parts of carnosine;

0.2-0.8 part of ascorbic acid;

2-5 parts of nicotinamide;

3-5 parts of a penetrating humectant;

2-10 parts of a forest blue lotion;

3-6 parts of rhamnose silanol;

0.3-1.5 parts of trehalose;

0.1-2 parts of dihydrooat acyl anthranilic acid;

0.1-1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer;

0.1-1 part of PEG-40 hydrogenated castor oil.

2. The polypeptide essence with the multi-effect whitening effect according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 68.1 parts of aloe extract, 1 part of reed extract, 1 part of licorice root extract, 1 part of angelica root extract, 1 part of salvia miltiorrhiza root extract, 1 part of dogwood fruit extract, 1 part of wolfberry root extract, 0.7 part of radix ophiopogonis extract, 1 part of rumex acetosa extract, 0.6 part of twisted cactus stem extract, 0.02 part of sophora flavescens ait root extract, 3 parts of 1, 2-pentanediol, nonapeptide-16 parts, 2 parts of carnosine, 0.3 part of ascorbic acid, 3 parts of nicotinamide, 3 parts of osmotic humectant, 2 parts of linlan lotion, 3 parts of rhamnose silanol, 0.8 part of trehalose, 1 part of dihydroavenoyl anthranilic acid, 0.1 part of acrylic acid (ester)/C12-22 alkanol methacrylate copolymer and 0.1 part of PEG-40 hydrogenated castor oil.

3. The polypeptide essence with multi-effect whitening effect according to claim 1, wherein: the rhamnosilanol is a mixture of 0.3 parts by weight of silanetriol, 2.35 parts by weight of rhamnose and 20 parts by weight of methylpropanediol.

4. The polypeptide essence with multi-effect whitening effect according to claim 1, wherein: the permeable humectant is a mixture of erythritol and creatinine HCl, and the mass ratio of the erythritol to the creatinine HCl is 100: 1.

5. The polypeptide essence with the multi-effect whitening effect according to claim 1, further comprising 0.1-1 weight part of a mixture of artemisia capillaris flower extract, clove flower extract and glyceryl caprylate; the mass ratio of the artemisia capillaris flower extract to the clove flower extract to the glyceryl caprylate is 20-25: 10-15: 3-5.

6. The polypeptide essence with multi-effect whitening effect according to claim 1, wherein the aloe extract is prepared by the following steps:

s1, selecting ripe aloe, cleaning, and pulping in a sealed environment;

s2, adding 5-8 times of 85-98% 1, 3-propylene glycol solution as solvent into the aloe pulp, performing ultrasonic treatment at 35-45 deg.C for 20min, and filtering to obtain the first filtrate;

s3, adding 10 parts by weight of distilled water into the obtained filter residue, adding degrading enzyme, stirring uniformly, standing for 30min, and filtering to obtain a second filtrate;

s4, mixing the first filtrate and the second filtrate, and mixing to obtain the aloe extract.

7. The polypeptide essence with multi-effect whitening effect according to claim 6, wherein: the degrading enzyme is a mixture of 0.15-0.2 weight parts of cellulase, 0.023-0.028 weight parts of pectinase and 0.015-0.018 weight parts of amylase.

8. The preparation method of the polypeptide essence with multi-effect whitening effect according to claim 1, which comprises the following steps:

a1, mixing Aloe extract, Phragmites communis extract and Glycyrrhrizae radix extract;

a2, mixing radix Angelicae sinensis extract, radix Salviae Miltiorrhizae extract, fructus Corni extract and fructus Lycii extract at room temperature;

a3, premixing 1, 2-pentanediol, nicotinamide and PEG-40 hydrogenated castor oil; and adding the mixture obtained in the step A1 and the step A2, mixing and stirring uniformly, adding nonapeptide-1, carnosine, a rumex acetosa extract, ascorbic acid, a penetrating humectant, a linlan lotion, rhamnose silanol, trehalose, a radix ophiopogonis extract, a twisted cactus stem extract, a sophora flavescens root extract, dihydroavenyl anthranilic acid and an acrylic acid (ester)/C12-22 alkanol methacrylate copolymer, stirring uniformly at normal temperature, standing stably and discharging.

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