KR101948597B1 - Hair cosmetic composition with excellent cleansing effect and soothing effect - Google Patents

Abstract

The present invention relates to a hair cosmetic composition, and more particularly, to a hair cosmetic composition containing an herbal complex fermentation extract with ingredients generating carbon dioxide when applied to hair or scalp, thus having an excellent cleaning and scalp soothing effect while lowering scalp heat. The present invention comprises a first composition comprising calcium carbonate, decyl glucoside, purified water, etc. and a second composition comprising lactic acid, guar hydroxy trimonium chloride, etc.

Description

[0001] The present invention relates to a hair cosmetic composition having excellent cleansing power and scalp soothing effect,

The present invention relates to a cosmetic composition for hair which is excellent in cleansing power and scalp soothing effect, and more particularly to a cosmetic composition for hair which not only has excellent washing power by generating carbon dioxide gas when applied to hair and scalp, but also has a soothing effect on scalp.

Recently, fine dust and dust cause serious health problems of the scalp. In particular, dust from China comes from heavy metals such as mercury and lead, and fine dust particles, which are much smaller than ordinary dust, tend to penetrate into the pores or follicles of the scalp. When the dust of the yellow dust clings to the scalp, the activity of the hair follicles is lowered, causing various problems such as dry scalp, itching and hair loss. Therefore, in order to prevent hair loss and itchy scalp caused by it, it is important to use a hair cosmetic excellent in detergency to remove dust and sebum accumulated on scalp and hair. However, there is a problem that the scalp stimulation may be caused by the chemical components used for increasing the washing power.

Recently, interest in using cosmetic products using carbonic acid is increasing. Carbonic acid is weakly acidic, which helps to maintain the acidity of the skin and is effective in removing wastes from pores. In addition, cosmetic products containing carbonic acid can produce bubbles when applied to the skin, so that a skin massage effect can be expected. However, people with dermatitis or sensitive skin may be able to irritate their skin, which can harm the health of the skin.

Accordingly, the present inventors have studied a cosmetic composition for hair that can relieve scalp irritation while having excellent washing power. In addition to components that generate carbon dioxide gas when applied to hair and scalp, it is based on traditional drug therapy, The present inventors completed the present invention by confirming that the herbal composition complex fermented extract prepared by mixing and fermenting the herbal medicines at an optimum ratio has excellent washing power and does not cause scalp irritation.

Korean Patent Publication No. 10-2010-0132664 (2010.12.20) Korean Patent No. 10-1616251 (May 2016)

The present invention aims to provide a cosmetic composition for hair which comprises a herbal mixed fermentation extract together with ingredients for generating carbon dioxide gas when applied to hair and scalp and has excellent washing power, but also low scalp heat and excellent scalp soothing effect.

In order to achieve the above object, according to the present invention, there is provided a one-component composition comprising calcium carbonate, decyl glucoside, lauryl glucoside, polyquaternium-10, guar hydroxy trichloromethane chloride and purified water; And a pharmaceutical composition comprising lactic acid, guar hydroxy trimerium chloride, herbal complex fermentation extract, and purified water.

Wherein the herbal mixed fermented extract is a first mixed fermentation extract of fig, ginkgo biloba, marine oil, seokchonpo, lobule, angelica, licorice, and ginger; A second mixed fermentation extract of ginseng, gold, safflower, persimmon, sugar, multi-leaf, omija and allium; And a third mixed fermented extract of ganoderma, ganoderma, ganoderma, Korean red ginseng, papaya, ginseng, black soybean, and ginseng.

Preferably, the first mixed fermentation extract is prepared by mixing the figs, ginkgo biloba, cornstarch, Seokchonpo, lobule, Angelica gigas, licorice and ginger at a weight ratio of 1: 2: 1: 1: 3: 2: 2, And the second mixed fermented extract is mixed with a mixture of ginseng, golden, safflower, ginseng, common sweet potato, multi-leaf, omija and allium at a weight ratio of 1: 3: 2: 1: 3: 3: 2: 3: 3: 3: 2: 2 in a weight ratio of 1: 3: 1: 3: 3: 3: 2: 2, and the third mixed fermentation extract is prepared by mixing ganoderma, ganoderma, It is extracted by fermentation with yeast.

The herbal combined fermentation extract as an active ingredient for lowering scalp heat and soothing the scalp is contained in an amount of 0.01 to 15% by weight based on the total weight of the pharmaceutical composition.

The cosmetic composition for hair may be any one selected from the group consisting of hair tonic, hair nourishing lotion, hair treatment, hair shampoo, hair rinse and hair lotion.

The cosmetic composition for hair of the present invention generates carbon dioxide gas when hair and scalp are applied, has excellent detergency, and contains a complex herbal extract to lower scalp heat and exhibit excellent scalp soothing effect.

FIG. 1 is a graph showing the effect of controlling the heating factor of the mixed fermentation extract according to the preparation example of the present invention.
FIGS. 2 and 3 are graphs showing the effect of controlling the hair growth inhibitory factor due to heat stress in the mixed fermentation extract according to the preparation example of the present invention.
FIG. 4 is a graph showing the cell protection effect of the mixed fermentation extract according to the preparation example of the present invention.
FIG. 5 is a graph showing the scalp soothing effect of the herbal complex fermentation extract according to the embodiment of the present invention. FIG.

Hereinafter, the present invention will be described in detail.

The present invention relates to a carbonic acid shampoo which generates carbon dioxide gas when applied to hair and scalp. It contains carbonic acid-generating ingredients together with a herbal extract which lowers scalp heat and produces scalp soothing effect. To a cosmetic composition for hair.

According to the present invention there is provided a one-part composition comprising calcium carbonate, decyl glucoside, lauryl glucoside, polyquaternium-10, guar hydroxytrimonium chloride and purified water; And a pharmaceutical composition comprising lactic acid, guar hydroxy trimerium chloride, herbal complex fermentation extract, and purified water.

When the hair cosmetic composition is applied to scalp and hair, carbon dioxide (carbon dioxide) is generated by reaction between the calcium carbonate contained in the composition and the lactic acid contained in the composition. This improves the effect of removing waste matter from the pores. In addition, when the generated carbon dioxide gas is absorbed into the scalp layer, the pH of the blood is decreased to promote dissociation of oxygen in hemoglobin and the surrounding tissue can obtain sufficient oxygen (Bohr effect) .

The decyl glucoside, lauryl glucoside, contained in the composition of the present invention is used as a surfactant for improving the detergency against hair and scalp. The guar hydroxytrimonium chloride contained in the compositions 1 and 2 is used as a viscosity controlling agent do.

According to one embodiment of the present invention, the one-part composition comprises 4 to 10% by weight of calcium carbonate, 30 to 40% by weight of decyl glucoside, 10 to 20% by weight of lauryl glucoside, 1 to 2% by weight of polyquaternium- 0.1 to 2% by weight of hydroxytrimonium chloride and the balance purified water. The composition for one preparation may further contain a flavoring agent, a hair conditioning agent, a preservative, and the like.

According to one embodiment of the present invention, the dentifrice composition comprises 5 to 10% by weight of lactic acid, 0.1 to 2% by weight of guarhydroxytrimonium chloride, 0.01 to 15% by weight of complex herbal extract and purified water of the remainder.

Wherein the herbal mixed fermented extract is a first mixed fermentation extract of fig, ginkgo biloba, marine oil, seokchonpo, lobule, angelica, licorice, and ginger; A second mixed fermentation extract of ginseng, gold, safflower, persimmon, sugar, multi-leaf, omija and allium; And a third mixed fermented extract of ganoderma, ganoderma, ganoderma, Korean red ginseng, papaya, ginseng, black soybean, and ginseng.

Each of the mixed fermented extracts is based on traditional pharmacotherapy, and herbal medicinal herbs proven by modern science are selected and extracted by mixed fermentation. Each of the mixed fermentation extracts may be an extract obtained by fermentation with yeast and then extracted with hot water or a mixture of water and ethanol extracted from water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, At least one solvent or mixed solvent selected from the group consisting of petroleum ether and diethyl ether. Extraction can be carried out by a conventional method.

According to one embodiment of the present invention, the fermented herbal herbs are put into an extraction container and refluxed for 30 minutes to 24 hours by using purified water, which is then filtered and concentrated.

The raw materials used in the preparation of the first mixed fermentation extract are those selected from herbal medicinal herbs which have been helpful for nutrition and blood circulation. When cell metabolic activity becomes inefficient, more heat is generated and it is difficult to treat the heat generated. Therefore, in order to improve the nutritional supply and blood circulation of the scalp,

Fig is a fruit of fig tree (Ficus carica L.), which is a deciduous bush tree of the mulberry tree, and has the efficacy of the fever of the fever and the detoxification of the fever. It is used for ringing, glare.

Ginkgo leaves are leaves of ginkgo biloba (Ginkgo biloba L.) which is a deciduous larva of Ginkgo biloba. Ginkgo biloba leaves are known to be good for heart and thrombus.

Cornus officinalis S. et Z. is a flesh of deciduous bush, Cornus officinalis S. et Z., which has the effect of bifurcation ginsenoside and convergent hyperlipidemia. It is used when the blood is broken and is deficient in Xinyang.

Seokchangpo is the roots of Acorus gramineus Soland, a herbaceous herbaceous plant of the genus Seongnam. It has the efficacy of Gyu-gyu Anshin, , Skin rashes and wrinkles, and bruises.

The leaves of Artemisia princeps var. Orientalis (Pampan.) Hara, Artemisia montana Pampan., And Artemisia argyi Lev. Et Vnt., Which are herbaceous plants of Asteraceae, It has the efficacy of hemostasis (热 经 止血), acidic pain (寒 痛 止痛), and dehumidifying itching (濕. 慢性). It is used for warming the blood of 气血 and gyeongmaek (经脉), skin eczema and itching.

Angelica gigas Nakai and Angelica acutiloba S. et Z. are medicinal products for the root of Angelica sinensis Dies, which is a herbaceous herbaceous plant, It has the efficacy of blood circulation (blood clotting blood), landscape circulation (tonic pain), circulation (潤腸 通 便), and promotes protein synthesis, promotes blood flow, promotes erythropoiesis, have.

Licorice is the roots of licorice (Glycyrrhzia uralensis Fisch.), Licorice and licorice (Glycyrrhzia inflata Bat.), Herbaceous perennial plants. It is taken in the spring and autumn to remove the hairy roots and dry in the sun. It has the effect of bobyuki (补脾 益气), cyanotic detoxification (淸 热 毒毒) .

Ginger is the roots of ginger (Zingiber officinale Rosc.), A herbaceous herb with ginger, and is the source of seaweed, Stopping cough), gentle seawater (寒痰 咳嗽) is effective, promotes blood circulation, anti-inflammatory and analgesic action.

1: 1: 1: 3: 2: 2 in the ratio of 1: 2: 1: 1: 1: 3: 2: 2 to the mixed fermentation extracts of figs, ginkgo leaves, , The best regulatory factor was shown.

The raw materials used in the preparation of the second mixed fermentation extract are selected from herbal medicinal herbs which have been helpful for cold and soothing effects since ancient times. The heat of the scalp weakens the power to catch the hair follicles by widening the pore that discharges heat. To improve this, we have provided the ingredient which helps the calming and inflammation relieving effect of the scalp.

Gosam is the roots of Sophora flavescens Ait. And Echinosophora koreensis Nakai, which are herbaceous perennial herbaceous plants, and are used for the treatment of eczema (eczema), eczema (eczema) ), And skin libido (skin pruritus) is effective, and itching of the skin, pustule windows, omnis or ringworm is used.

Golden (黄 芩) is a root of the herbaceous plant (Scutellaria baicalensis Georgi), which is a perennial herbaceous plant of the family Lamiaceae. It is a root of the herbaceous plant, It has the efficacy, it regulates the sooting heat (阳阳 經热) and treats the skin inflammation reaction.

The safflower is a flower of the safflower (Carthamus tinctorius L.), a herbaceous plant of the Asteraceae family. It has the efficacy of a vasculopathic route and a mountainous vaginal discharge, a polymorphic erythema treatment, Treat it.

Hwangseongcho is the underside of Hwangmokmil (Houttuynia cordata Thunb.), A herbaceous plant of Saururus chinensis, and has the efficacy of Cheongryeok detoxification, drainage and diuretic trophy , The action of releasing pus (pus) while lowering the heat, and the symptoms of fever, urination, and pain.

It is the roots and the ground of Swertia pseudo-chinensis (Bunge) Hara which is a herbaceous plant, which is one and a half year old of Yongdam. It has the efficacy of blue fever detoxification, It acts on the surface, digestion, and anti-inflammation.

Tea leaves are leaves of the tea tree (Thea sinensis L.) which is an evergreen bush of the tea plant, and it is a leaf of Chengdu tree, manganese, manganese, Diuretic), detoxification (detoxification), has the effect of liver function, detoxification.

Omiza is a mature fruit of Schizandra chinensis Baill which is a deciduous vine tree with magnolia and is the fruit of convergence zodiac, 益气 生 津, and bosin ginseng. And it improves the convergence action, the generation action of 津液 (fluid), the brain power, and the intelligence.

It is a fruit of Forsythia saxatilis Makai which is a deciduous bush of the aspira tree. It has the efficacy of blue fever detoxification, Acting, and releasing heat.

 The extracts were mixed and blended in various ratios. As a result, the mixture was fermented and extracted at a weight ratio of 1: 3: 2: 1: 3: 3: 2: 2 of gosam, golden, safflower, The best skin soothing effect was obtained.

The raw materials used for the production of the third mixed fermentation extract are selected from oriental herbal medicines which have been used for controlling the function of the kidney, In order to improve the kidney function, we can not prevent the elevation of the scalp due to a problem with the heat control function of the kidney. Therefore, we need to maintain the body's homeostatic function and provide a component Respectively.

Ganoderma lucidum is the surface of the mushroom (Ganoderma lucidum Karst) with molluscs and has the efficacy of huro (虚勞), sea cucumber, 气 喘, 眠 (ぬ 眠), and news (eradication) , Regular (精 气) enhancement, has the effect of liver activation.

Curcuma aromatica Salish is a root of ginger and herbaceous herbaceous plant Curcuma aromatica Salish. It is known for its vasodilatosis, It has the efficacy of Cheongshim Gyu Gyu (淸 心 開竅) and Ehād Emotion (膽 膽 黄), and it activates the hungry wool (气 气 解压), Cheongryeol (淸 熱)

Chrysanthemum zawadskii var. Alpinum Kitamura, Chrysanthemum zawadskii var. Tenuisectum Kitamura, Chrysanthemum var. Chrysanthemum, Chrysanthemum zawadskii var. It is the top part of zawadskii Herbich ssp. naktongense (Nakai) Y. Lee, stat. nov), and it treats uterine hysterectomy by Ianjeon and landscaping, .

The Red Horsetail is a dried root of Polygonum multiflorum Thunb., Which is effective for blood circulation. It has a detoxifying effect and a skin irritation Lt; / RTI >

Leaves of leaves of Thaja orientalis L., Thuja occidentalis L., Chamaecyparis pisifera (S. et Z.), and leaves of blooming hemorrhagic fever Blood hemostasis), the effect of (祛痰 止咳) is effective, the symptoms of falling hair, seborrheic dermatitis is used.

Hansikcho is an underside of the herbaceous plant Eclipta prostrata L. which is an annual plant of Asteraceae. It has the efficacy of consonant ixin (滋阴 益 肾), blood hemostasis (慢血 止血) It is used for symptoms that have no force on the knee.

Black beans and black beans are seeds of black beans (Glycine max Merr), a herbaceous herb plant of soybean, and have been shown to be effective in the treatment of hibernation, iso, giant wind, and detoxification. There is a strong wind (wind) action, detoxification.

Ginseng is the root of ginseng (Panax ginseng CA Mayer), which is a herbaceous plant of Oriental herbaceous plant, and has been widely used as a medicinal herb in many fields such as Daeboenggi, Bibiwikung, , Anshin, and 增志. It has an effect of producing 津液, and it supports the blood of 气血.

The extracts were mixed with each other at various ratios and the extracts were mixed and fermented at a weight ratio of 1: 3: 1: 3: 3: 3: 2: 2 in the ratio of Yongji, , It showed the best control of fever factors and scalp soothing effect.

When the first mixed fermentation extract, the second mixed fermentation extract and the third mixed fermentation extract are used in combination, compared with the case of using each of the mixed fermentation extracts, various parts, that is, Inhibitory effect on growth inhibitory factor expression, cell protection, sedation and the like. The mixing ratio of each of the mixed extracts is not limited, but it is preferable to mix them at the same weight ratio.

Since the cosmetic composition for hair of the present invention contains the herbal complex complex fermented extract, it effectively alleviates skin irritation caused by carbon dioxide gas generated when using cosmetic hair cosmetics, and exhibits excellent scalp and hair protecting activity because it lowers scalp heat.

The compositions 1 and 2 are used to be mixed with each other when applied to scalp and hair. The first composition and the second composition may be stored in respective individual containers and mixed in a certain ratio at the time of use. Preferably, the first composition and the second composition are stored in a single container divided into partitions, So that bubbles are formed and used.

The hair cosmetic composition may be any one selected from the group consisting of hair tonic, hair nourishing lotion, treatment, hair shampoo, hair rinse, and hair lotion.

In addition, the hair cosmetic composition of the present invention may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, As used herein means any emulsion or emulsion which is commonly used in emulsifying or emulsifying agents, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics May contain adjuvants such as other ingredients. And the above ingredients may be introduced in amounts commonly used in the dermatology field.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Production Examples 1 to 3: Preparation of first mixed fermentation extract

Dried figs, bank leaves, corn oil, Seokchangpo, lobster, Angelica gigas, licorice and ginger were mixed at the ratios shown in Table 1, and fermented at 35 ° C for 24 hours in a kneader dough corresponding to twice the dry weight, The mixed natural material was subjected to reflux extraction at 100 DEG C for 2 hours in purified water having a dry weight of 10 times. This was concentrated under reduced pressure and lyophilized to prepare a first mixed fermented extract.

(g) FIG Bank Corn oil Seokchangpo Lady Angelica licorice ginger Production Example 1 4 2 2 2 2 4 4 6 Production Example 2 2 4 2 2 2 6 4 4 Production Example 3 2 2 4 2 2 4 6 4

Test Example 1: Regulatory effect of recessive TRPV-1 expression

Transient receptor potential vanilloid 1 (TRPV1) expression levels were measured for the mixed fermentation extracts prepared in Preparation Examples 1 to 3. HHFK (Human hair follicular keratinocytes) cells were seeded at a density of 1 × 10 ⁶ cells / well in a 6-well plate and cultured in Keratinocyte medium for 24 hours at 37 ° C in a 5% CO ₂ incubator. HHFK cells were treated with 200 ug / ml of each sample, incubated for 24 hours, and exposed to high temperature (? 43 ° C) for 3 hours to induce thermal stress. After 24 hours of recovery time, RNA was extracted from the cells and RT-PCR was performed to measure TRPV1 gene expression level. The results are shown in Table 2.

division Normal cell group Heat-treated cells Override Override Production Example 1 Production Example 2 Production Example 3 TRPV1 expression (%) 100% 128% 115% 109% 113%

As shown in Table 2, the TRPV1 gene expression inhibitory effect was found to be 1: 2: 1: 1 in the extracts of the preparation of the present invention, and that of figs, ginkgo biloba, : 1: 3: 2: 2, and showed the most excellent effect in Preparation Example 2 extracted.

Production Examples 4 to 6: Preparation of the second mixed fermentation extract

The mixture of dried ginseng, golden, safflower, persimmon, licorice, multilineal, omija and allium were mixed at the ratios shown in Table 3, and then fermented at 35 ° C for 24 hours in a kneader dough corresponding to twice the dry weight, The natural product was subjected to reflux extraction at 100 DEG C for 2 hours in purified water having a dry weight of 10 times. This was concentrated under reduced pressure and lyophilized to prepare a second mixed fermented extract.

(g) GoSam Gold Safflower Sophora Generic Multi-leaf Schisandra Abalone Production Example 4 4 2 6 6 4 4 2 6 Production Example 5 2 4 2 4 2 2 6 2 Production Example 6 2 6 4 2 6 6 4 4

Test Example 2: Modulating effect of TGF-beta2, a hair growth inhibitory factor

The amount of expression of TGF-beta2 (Transforming growth factor-beta2), which is a hair growth inhibitory factor, was measured for the mixed fermentation extracts prepared in Production Examples 4 to 6. [ HHFK (Human hair follicular keratinocytes) cells were seeded at a density of 1 × 10 ⁶ cells / well in a 6-well plate and cultured in Keratinocyte medium for 24 hours at 37 ° C in a 5% CO ₂ incubator. HHFK cells were treated with 200 ug / ml of each sample, incubated for 24 hours, and exposed to high temperature (? 43 ° C) for 3 hours to induce thermal stress. After 24 hours of recovery, RNA was extracted from the cells and RT-PCR was performed to measure TGF-β2 gene expression level. The results are shown in Table 4.

division Normal cell group Heat-treated cells Override Override Production Example 4 Production Example 5 Production Example 6 TGF-β2 expression (%) 100% 127% 117% 115% 111%

As can be seen in Table 4, in all of the extracts of the present invention, TGF-β2 And the mixture was fermented at a weight ratio of 1: 3: 2: 1: 3: 3: 2: 2 in the ratio of 1: 3: 2: 2, The best results were obtained.

Production Examples 7 to 9: Production of the third mixed fermentation extract

The dried ganoderma, Ulugumi, Rhus javanica, Korean red ginseng, Paeonia japonica, Hanshik, Korean black ginseng and Ginseng were mixed at the ratios shown in Table 5 below and then fermented at 35 ° C for 24 hours in the yeast dough corresponding to twice the dry weight, The mixed natural material was subjected to reflux extraction at 100 DEG C for 2 hours in purified water having a dry weight of 10 times. This was concentrated under reduced pressure and lyophilized to prepare a third mixed fermented extract.

(g) wisdom Kool Necklace Enemy Pyeongbyeop Hansikcho Black beans Ginseng Production Example 7 6 2 6 4 2 4 2 6 Production Example 8 4 4 4 2 4 2 6 2 Production Example 9 2 6 2 6 6 6 4 4

Test Example 3: Modulating effect of DKK-1, a hair growth inhibitory factor

The amount of DKK-1 (Dickkopf-1) expressed as a hair growth inhibitory factor was measured for the mixed fermentation extracts prepared in Preparation Examples 7 to 9. HHFK (Human hair follicular keratinocytes) cells were seeded at a density of 1 × 10 ⁶ cells / well in a 6-well plate and cultured in Keratinocyte medium for 24 hours at 37 ° C in a 5% CO ₂ incubator. HHFK cells were treated with 200 ug / ml of each sample, incubated for 24 hours, and exposed to high temperature (? 43 ° C) for 3 hours to induce thermal stress. After 24 hours of recovery time, RNA was extracted from the cells and RT-PCR was performed to measure DKK-1 gene expression level. The results are shown in Table 6.

division Normal cell group Heat-treated cells Override Override Production Example 7 Production Example 8 Production Example 9 DKK-1 expression (%) 100% 126% 118% 117% 114%

As can be seen from the above Table 6, in all of the extract samples of the preparation of the present invention, DKK-1 And the extracts were mixed and fermented at a weight ratio of 1: 3: 1: 3: 3: 3: 2: 2 in the ratio of 1: 3: 3: 3: 2: 9 showed the best effect.

Production Examples 10 to 13: Production of Liquid Raw Material Containing Mixed Fermentation Extract

The liquid raw materials containing the mixed fermentation extracts of Preparation Example 2, Preparation Example 6 and Preparation Example 9, which were confirmed as the most preferable mixed fermentation extract in the above Test Example, and the herbal combined fermentation extracts obtained by mixing them at the same weight ratio, The composition and the content were prepared by a conventional method.

ingredient Content (% by weight) Control Example Production Example 10 Production Example 11 Production Example 12 Production Example 13 Production Example 2 - 2.42 - - - Production Example 6 - - 2.42 - - Production Example 9 - - - 2.42 - Herbal complex fermentation extract - - - - 2.42 Purified water 100 Remainder Remainder Remainder Remainder Sum 100 100 100 100 100

Test Example 4: Modulating effect of TRPV-1, TGF-beta2 and DKK-1 on liquid raw materials containing mixed fermented extract

In order to examine the effect of regulating the heat and heat stress on the liquid raw material containing the mixed fermented extract prepared in the above Preparation Example, transient receptor potential vanilloid 1 (TRPV1) and hair growth TGF-β2 (Transforming growth factor-β2) and DKK-1 (Dickkopf-1) expression levels were measured. HHFK (Human hair follicular keratinocytes) cells were seeded at a density of 1 × 10 ⁶ cells / well in a 6-well plate and cultured in Keratinocyte medium for 24 hours at 37 ° C in a 5% CO ₂ incubator. HHFK cells were treated with 1% of each sample and 10 uM of Capsazepine as a positive control. After 24 hours of incubation, the cells were exposed to high temperature (≥43 ° C) for 3 hours to induce thermal stress. RT-PCR was performed to extract TRPV1, TGF-β2 and DKK-1 gene expression levels. The results are shown in Table 8.

division normal
Cell group Heat-treated cells Override Control Example Manufacturing example 10uM Capsazepine 10 11 12 13 TRPV1 expression (%) 100% 127% 109% 115% 113% 105% 103% TGF-β2 expression (%) 100% 126% 117% 111% 115% 106% 110% DKK-1 expression (%) 100% 125% 118% 117% 114% 106% 105%

As can be seen from the above Table 8, the liquid raw material of Production Example 10 containing the first mixed fermentation extract of Preparation Example 2 showed a comparatively high effect of regulating the enthusiasm and the second mixed fermentation extract of Preparation Example 6 The liquid raw material of Production Example 12 containing the liquid raw material of Production Example 11 and the third mixed fermentation extract of Production Example 9 showed a relatively high effect of controlling hair growth inhibitory factor due to heat stress. In the liquid raw material of Production Example 13 containing all of the first, second and third mixed fermentation extracts, the synergistic effect exerts the most excellent effects in both the regulatory effect of heat factors and the effect of regulating hair growth inhibitory factors due to heat stress I could confirm. 1 to 3.

Test Example 5: Cytoprotective effect on liquid raw materials containing mixed fermentation extract

In order to confirm the cytoprotective effect of the liquid raw material containing the mixed fermentation extract of Preparation Example 13, cell changes were observed. HHFK (Human hair follicular keratinocytes) cells were seeded at a density of 1 × 10 ⁶ cells / well in a 6-well plate and cultured in Keratinocyte medium for 24 hours at 37 ° C in a 5% CO ₂ incubator. HHFK cells were treated with 1% of the extract of Preparation Example 13, and cultured for 24 hours, then exposed to high temperature (? 43 ° C) for 5 hours to give heat stress. Cells were observed and photographed using a microscope.

The group treated with Preparation Example 13 containing the herbal combined fermentation extract of the present invention showed a better cell protection effect than the untreated group, This is shown in FIG.

Example 1: Preparation of one-component composition

A one-part composition was prepared according to a conventional method with the composition and content shown in Table 9 below.

ingredient (weight%) Guahydroxytrimonium chloride 0.5 Polyquaternium-10 1.2 Calcium carbonate 5.0 Decyl glucoside 35.0 Lauryl glucoside 10.0 Sodium cocoylalaninate 20.0 Sodium lauryl glucose carboxylate 15.0 Cocamidopropyl betaine 10.0 When tetrasodium is added to the dithia a very small amount Incense, preservative a very small amount Purified water Balance system 100

Example 2: Preparation of binary composition

The fugitive composition containing the extract of Preparation Example 13 identified as the most preferable mixed fermentation extract in the above Test Example was prepared according to a conventional method with the composition and content shown in Table 10 below. And a sample not containing it was determined as Comparative Example 1.

ingredient (weight%) Example 2 Comparative Example 1 Guahydroxytrimonium chloride 0.5 0.5 Lactic acid 6.0 6.0 Mixed fermented extract (Preparation Example 13) 1.0 - Sodium cocoylalaninate 20.0 20.0 Sodium lauryl glucose carboxylate 17.0 17.0 Cocamidopropyl betaine 10.0 10.0 When tetrasodium is added to the dithia a very small amount a very small amount PGE-150 Pentaerythritol tetrastearate a very small amount a very small amount PIGGY-40 Hydrogenated Castor Oil a very small amount a very small amount Incense, preservative a very small amount a very small amount Purified water Balance Balance system 100 100

Test Example 6: Confirming scalp soothing effect

To examine the scalp soothing effect of the hair cosmetic composition of Example 2, 10 healthy adults aged 20 to 50 years were tested.

In order to confirm the soothing effect of the scalp, each 5 persons were irradiated with infrared ray for 30 minutes on a flat part of the back neck to induce heat sensation. Then, the formulations of Comparative Examples 1 and 2 were applied, And absorbed by standing. Thereafter, erythema and irritation due to heat irradiation at the application site were compared and evaluated with time. The evaluation was carried out using the Aram 300 lens camera at room temperature and humidity of 50 ± 10% RH at a room temperature of 22 ± 2 ° C, and one researcher measured it for objective measurement.

As shown in FIG. 5, the cosmetic composition of Example 2 containing the mixed fermented extract of the present invention exhibited a soothing effect as compared with Comparative Example 1.

Test Example 7: Detergency and soothing effect of the scalp

30 adults aged 20 to 50 years were divided into two groups to evaluate the cleansing power and scalp soothing effect of the present invention cosmetic composition for hair.

In one group, the first composition of Example 1 and the second composition of Example 2 were mixed at the same weight ratio once daily to form bubbles and used.

In the other groups, the composition of Example 1 and the composition of Comparative Example 1 were mixed at the same weight ratio once a day to form bubbles and used. The state after 2 weeks of use was evaluated, and the results are shown in Table 11 below.

Item Example 1 + Example 2 Example 1 + Comparative Example 1 The scalp is not dry 4.3 3.0 There is no itching. 4.1 2.5 Have good cleaning power 4.2 4.2

(Standard: 5 - very good 4 - good 3 - normal 2 - not good 1 - not very good.

As shown in Table 11, when the two-component composition containing the complex herbal extract of the present invention was used together with the one-component composition, it showed excellent detergency and no skin irritation, and when used with the composition of Comparative Example 1 It has an excellent scalp soothing effect.

Claims (7)

1 composition comprising calcium carbonate, decyl glucoside, lauryl glucoside, polyquaternium-10, guar hydroxytrimonium chloride, and purified water; And a detergent composition comprising lactic acid, guar hydroxytrimonium chloride, herbal complex fermentation extract and purified water,
Wherein the herbal mixed fermented extract is a first mixed fermentation extract of fig, ginkgo biloba, marine oil, seokchonpo, lobule, angelica, licorice, and ginger; A second mixed fermentation extract of ginseng, gold, safflower, persimmon, sugar, multi-leaf, omija and allium; And a third mixed fermented extract of ganoderma, ganoderma, ganoderma, red ginseng, papaya, japonica, black soybean, and ginseng. delete The method according to claim 1, wherein the first mixed fermented extract is prepared by mixing fig, ginkgo biloba, Seokjangpo, lagophen, Angelica gigas, licorice and ginger at a weight ratio of 1: 2: 1: 1: 1: 3: 2: 2 And then extracted by fermentation with koji. The method according to claim 1, wherein the second mixed fermented extract is prepared by mixing ginseng, golden, safflower, ginseng, common sugar, multifloral, omija and alfalfa in a weight ratio of 1: 3: 2: 1: 3: 3: 2: 2 , And extracted by fermentation with koji. The method according to claim 1, wherein the third mixed fermented extract is a mixture of ganoderma, ganoderma, ganoderma, red ginseng, And then extracted by fermentation with koji. [2] The cosmetic composition for hair according to claim 1, wherein the herbal combined fermentation extract is contained in an amount of 0.01 to 15% by weight based on the total weight of the composition. [2] The cosmetic composition for hair according to claim 1, wherein the cosmetic composition for hair has one of the formulations selected from the group consisting of hair tonic, hair nourishing lotion, scarf treatment, hair treatment, hair shampoo, hair rinse and hair lotion A cosmetic composition for hair.

Images