CN113599330A

CN113599330A - Compound anti-aging firming polypeptide skin care product and preparation method thereof

Info

Publication number: CN113599330A
Application number: CN202111012873.0A
Authority: CN
Inventors: 陈坤
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-31
Filing date: 2021-08-31
Publication date: 2021-11-05

Abstract

The invention discloses a compound anti-aging firming polypeptide skin care product and a preparation method thereof, wherein the compound anti-aging firming polypeptide skin care product comprises the following raw materials in parts by weight: 0.001-0.01 part of carnosine, 10-0.0008 part of tripeptide, 0-0.0007 part of acetyl tetrapeptide-90.0002, 0-0.0008 part of acetyl tetrapeptide-20.0003, 0.0008 part of hexapeptide-90.0002-0.0007 part of swertia extract, 0-5 parts of beta-glucan, 0-5 parts of recombinant collagen, 8-10 parts of butanediol, 0.5-0.8 part of 1, 2-hexanediol, 0.3-0.5 part of carissone, 0-5 parts of deep sea fish skin collagen, 10-10 parts of biosugal-40-8 parts of biosugal-40 parts of water and 50.2-73.2 parts of water. The skin care product prepared by adopting the components can effectively remove static lines on the face, resist aging, brighten skin color, resist wrinkle and keep moisture.

Description

Compound anti-aging firming polypeptide skin care product and preparation method thereof

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to a compound anti-aging skin-firming polypeptide skin care product and a preparation method thereof.

Background

Except for aging, the main factors of skin aging are free radical oxidation, ultraviolet ray, radiation and environmental pollution, which accelerate the generation of free radicals in the body, further accelerate skin aging, lack of elasticity and dull skin color. The antioxidant substances are used for eliminating free radicals, so that the purpose of delaying skin aging is achieved. In addition, people cannot prevent the gradual lapse of time, people cannot exclamate the past and resist aging when passing years, do not pay attention to skin care when the people are young, do exercises of various intentions such as staying up at night, irregular diet and the like when the people are on the basis of the greatest money of youth, and regret after the years.

The polypeptide, also called victory peptide, is the most expensive component in the anti-aging three-giant head, has more mild factors than the factors of vitamin A and vitriol, basically has no side effect, does not need to establish tolerance, can be used for sensitive muscle, but has longer effective time. The nature of a polypeptide is a small fragment or basic component of a protein and is a crucial part of the functioning of a protein. Intact, non-fragmented proteins (e.g., collagen) are not absorbed through the epidermal layers of the skin. The polypeptides added in the skin care product are basically artificially modified, so that the polypeptides have smaller molecular weight and skin-friendly property, thereby promoting permeation and absorption.

The skin will repair automatically when there is a wound, this "automatic" is the signal required, the polypeptide plays the role of the signal, and once the signal is reached, the cells of the skin will operate as required. However, with age, natural aging plus environmental pollution, especially photoaging, some "signs" in the skin decline and fail, and in this time, signals are often generated by "supplementary" polypeptides. The signal-playing polypeptide has the effects of preventing elastin from decomposing and promoting collagen synthesis, mainly aims at dynamic and static veins in the aspect of anti-wrinkle, and has an obvious effect on dry, elastic and loose skin. In addition, the mechanism of action of the polypeptide is to block communication between nerves and muscles, reducing muscle wear, thereby allowing slower wrinkle formation, such as crow's feet, forehead lines, smiley expression lines, similar to jonit needles. Some polypeptides have the function of transporting important trace elements (such as copper ions, manganese ions and the like) to important enzyme reactions, and play an important role in promoting the synthesis of collagen and elastin, wound healing and other functions

In addition, Carnosine (L-Carnosine), a known name of β -alanyl-L-histidine, is a crystalline solid composed of two amino acids, β -alanine and L-histidine. Muscle and brain tissues contain very high concentrations of carnosine. Carnosine is a peptide found by the russian chemist gule vicker together with carnitine. Studies in the uk, korea, russia and other countries have shown that: carnosine has strong antioxidant capacity and is beneficial to human body. Carnosine has been shown to scavenge reactive oxygen Radicals (ROS) and α - β unsaturated aldehydes, which form during oxidative stress over-oxidation of cell membrane fatty acids. Mainly utilizes the strong capability of eliminating free radicals and the antioxidation.

Although it is said through research that: skin care products containing carnosine and polypeptides have antioxidant and anti-aging effects on human skin, but the antioxidant, anti-aging and facial static pattern removal effects are poor.

Disclosure of Invention

In view of the above, the present invention aims to provide a compound anti-aging skin-firming polypeptide skin care product which can effectively remove static lines on the face, resist aging, brighten skin color, resist wrinkle and preserve moisture.

The invention also aims to provide a preparation method of the compound anti-aging firming polypeptide skin care product.

In order to solve the problems, the technical scheme adopted by the invention is that the compound anti-aging firming polypeptide skin care product comprises the following raw materials in parts by weight:

0.001-0.01 part of carnosine, 10-0.0008 part of tripeptide, 0-0.0007 part of acetyl tetrapeptide-90.0002, 0-0.0008 part of acetyl tetrapeptide-20.0003, 0.0008 part of hexapeptide-90.0002-0.0007 part of swertia extract, 0-5 parts of beta-glucan, 0-5 parts of recombinant collagen, 8-10 parts of 1, 3-butanediol, 0.5-0.8 part of 1, 2-hexanediol, 0.3-0.5 part of carissone, 0-5 parts of deep-sea fish skin collagen, 10-10 parts of biogum glycocoll, 40-8 parts of biogum glycocoll and 50.2-73.2 parts of water.

Preferably, the skin care product comprises the following raw materials in parts by weight:

0.003 to 0.008 part of carnosine, 0.0007 part of tripeptide-10.0005 to 0.0007 part of acetyl tetrapeptide-90.0003 to 0.0006 part of acetyl tetrapeptide-20.0005 to 0.0007 part of acetyl tetrapeptide-90.0003 to 0.0006 part of hexapeptide-90.0003 to 3 parts of swertia extract, 3.2 to 5 parts of beta-glucan, 3.2 to 5 parts of recombinant collagen, 8.5 to 10 parts of 1, 3-butanediol, 0.55 to 0.8 part of 1, 2-hexanediol, 0.35 to 0.5 part of carissone, 3.2 to 5 parts of deep sea fish skin collagen, 18.5 to 10 parts of biosaccharide-46.5 to 8 parts of biosaccharide-46 parts of water and 52.6894 to 64.7954 parts of water.

0.005 part of carnosine, 0.005 part of tripeptide-10.0005 part, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep sea fish skin collagen, 110 parts of biogum, 48 parts of biogum and 50.2 parts of water.

Preferably, the carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and hexapeptide are all 95% or more pure.

The second technical scheme of the invention is realized as follows: a preparation method of the compound anti-aging skin-firming polypeptide skin care product is realized by the following steps:

s1, respectively weighing the following components in parts by weight: carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2, hexapeptide-9, swertia extract, beta-glucan, recombinant collagen, 1, 3-butanediol, 1, 2-hexanediol, menthone, deep sea fish skin collagen, biological carbohydrate gum-1, biological carbohydrate gum-4 and water;

s2, adding the 1, 3-butanediol into water, and stirring to dissolve the 1, 3-butanediol to obtain a 1, 3-butanediol aqueous solution;

s3, adding a swertia japonica extract, beta-glucan, bioglycan-1 and bioglycan-4 into the 1, 3-butanediol aqueous solution, and stirring to fully dissolve the materials to obtain a first mixed solution;

s4, adding recombinant collagen, 1, 2-hexanediol, jasminodin and deep-sea fish skin collagen into the first mixed solution, and stirring to fully dissolve the recombinant collagen, the 1, 2-hexanediol, the jasminodin and the deep-sea fish skin collagen to obtain a second mixed solution;

s5, adding carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and hexapeptide-9 into the second mixed solution, stirring uniformly, standing, filtering, and checking to be qualified to obtain the compound anti-aging and firming polypeptide skin care product.

Preferably, the specific method for stirring in S2 is as follows: firstly, stirring for 5-10 min under the condition that the stirring speed is 50-60 r/min; and then stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min.

Preferably, the specific method for stirring in S3 is as follows: firstly, stirring for 10-30 min under the condition that the stirring speed is 60-80 r/min; and then stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min.

Preferably, the specific method for stirring in S4 is as follows: stirring for 10-20 min under the condition that the stirring speed is 30-45 r/min.

Preferably, the specific method for stirring in S5 is as follows: stirring the mixture evenly at a stirring speed of 30-45 r/min.

Preferably, in the step S5, the standing time is 1-2 h; and during filtering, a 500-1000 mesh filter screen and a laminate filter are adopted for filtering.

Compared with the prior art, the compound anti-aging skin-firming polypeptide skin care product obtained by selecting the components can effectively adjust the number of static lines on the face, improve the synthesis of collagen, increase the skin elasticity, greatly improve the skin surface glossiness, make the skin color uniform, improve the yellowing, and have certain functions of brightening the skin color, tendering the skin and removing wrinkles.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

It is to be noted that all the components used in the following examples are commercially or self-made.

The watercress extract used in the following examples was prepared as follows.

The carnosine, the tripeptide-1, the acetyl tetrapeptide-9, the acetyl tetrapeptide-2 and the hexapeptide in the embodiment of the invention are obtained by the following methods:

firstly, according to a material-liquid ratio of 1: 9 (carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 or hexapeptide) and pure water are mixed and soaked for about 1h, stirred at the stirring speed of 4000r/min, and then membrane-filtered to obtain the product.

The embodiment of the invention provides a compound anti-aging firming polypeptide skin care product which comprises the following raw materials in parts by weight:

In the specific implementation process, the skin care product can be prepared by weighing the following raw materials in parts by weight:

Furthermore, the purity of carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and hexapeptide is 95% or more.

In addition, the swertia extract is the whole herb of swertia pseudochinensis Franch of Gentianaceae, and has the chemical name: swertia bimaculata (Sieb.et Zucc.) hook.f.et Thom, and the specific extraction method comprises the following steps: firstly, crushing the whole plant and drying in the shade to ensure that the moisture content is not higher than 12%, then uniformly spraying strains on the plant for fermentation, turning the swertia herb materials once every day, circularly and aseptically blowing air once every 30min, controlling the temperature to be 37.5-42 ℃, and keeping the relative humidity between 80-90%; after 7 days of fermentation, the mixture is fermented according to the material-water ratio of 1: 10 mixing the fermented material of the swertia pseudochinensis and water, extracting with water for about 4h, soaking at 90-100 deg.C, preferably at 95 deg.C, sieving with 500 mesh sieve, and centrifuging in a high speed centrifuge (17500 r/min).

The beta-glucan is a product from fermentation, the strain of the beta-glucan is from electrolyte-resistant beach, biochemical enzyme digestion is carried out after glycogen fermentation, and the final molecular weight distribution is 5000-.

The recombinant collagen is a collagen which is based on the original gene sequence of human type III collagen, selects a part with strong water solubility and high biological activity to carry out codon optimization and recombination, adopts an escherichia coli expression system, and realizes large-scale production by utilizing a biological fermentation technology, and has the characteristics of 100 percent homology with human, strong water solubility, high biological activity, high transdermal absorption rate and the like. The collagen has unique flexibility of 164.88 degrees of triple helix, and the structure can obviously enhance the adhesion of the collagen to cells, and the final molecular weight distribution is required to be 800-1500Dalton, so that the collagen can be prepared.

The deep-sea fish skin collagen is collagen extracted from deep-sea fish skin serving as a raw material. Due to the low temperature and low pressure of deep sea, the collagen has special properties which are not possessed by common terrestrial animal collagen and collagen of freshwater fish and shallow sea fish, and the activity of the collagen extracted from terrestrial animal bodies can be fully excited at 35 ℃. I.e. absorption above 35 degrees is optimal. The activity excitation temperature for extracting collagen from deep-sea fish is much lower, and the best effect can be achieved at more than ten degrees. The skin care product for external use is preferably extracted from deep sea fish. The final molecular weight distribution is required to be 700-.

The biological carbohydrate gum is a linear polysaccharide viscous solution, and is obtained by performing bacterial fermentation on a corn raw material through a biological process. The biological sugar gum is an excipient and an active substance, can endow the skin with double effects, effectively improves the skin feel, can form a film on the surface of the skin, and brings a moisturizing effect to the skin. And through the interaction of the polysaccharide and a keratinocyte receptor, inflammation and allergy are regulated, the activity of a longevity factor is promoted, and the differentiation of keratinocyte is promoted. The multifunctional active substance has unique film forming effect, and can provide 2-in-1 moisturizing effect to skin. The final molecular weight distribution is required to be 200-800Dalton, the content of L-fucose is more than or equal to 2.5 percent, and the content of L-rhamnose is more than or equal to 3.0 percent.

The embodiment of the invention also provides a preparation method of the compound anti-aging firming polypeptide skin care product, which is realized by the following steps:

s1, weighing the following components in parts by weight: carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2, hexapeptide-9, swertia extract, beta-glucan, recombinant collagen, 1, 3-butanediol, 1, 2-hexanediol, menthone, deep sea fish skin collagen, biological carbohydrate gum-1, biological carbohydrate gum-4 and water;

s2, adding the 1, 3-butanediol into water, and stirring to dissolve the 1, 3-butanediol to obtain a 1, 3-butanediol aqueous solution; the specific stirring method comprises the following steps: firstly, stirring for 5-10 min under the condition that the stirring speed is 50-60 r/min; stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min;

s3, adding a swertia japonica extract, beta-glucan, bioglycan-1 and bioglycan-4 into the 1, 3-butanediol aqueous solution, and stirring to fully dissolve the materials to obtain a first mixed solution; the specific stirring method comprises the following steps: firstly, stirring for 10-30 min under the condition that the stirring speed is 60-80 r/min; stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min;

s4, adding recombinant collagen, 1, 2-hexanediol, jasminodin and deep-sea fish skin collagen into the first mixed solution, and stirring for 10-20 min at a stirring speed of 30-45 r/min to fully dissolve the recombinant collagen, the 1, 2-hexanediol, the jasminodin and the deep-sea fish skin collagen to obtain a second mixed solution;

s5, adding carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and hexapeptide-9 into the second mixed solution, stirring uniformly at a stirring speed of 30-45 r/min, standing for 1-2 h, filtering by using a 500-1000 mesh filter screen and a laminate filter, and checking to be qualified to obtain the compound anti-aging skin-firming polypeptide skin care product.

The following are specific examples

Example 1

The compound anti-aging firming polypeptide skin care product provided by the embodiment 1 specifically comprises the following raw materials in parts by weight:

0.005 part of carnosine, 10 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep-sea fish skin collagen, 110 parts of biogum glycocoll, 48 parts of biogum glycocoll and 53.494 parts of water.

The compound anti-aging skin-firming polypeptide skin care product provided by the embodiment 1 is obtained by the following method:

1) adding 53.494 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 8min at a stirring speed of 50 r/min; stirring for 4min at a stirring speed of 2000r/min to fully dissolve the mixture to obtain a 1, 3-butanediol aqueous solution;

2) continuously adding 3 parts of swertia extract, 5 parts of beta-glucan, 110 parts of biological carbohydrate gum and 1 part of biological carbohydrate gum-48 into 1, 3-butanediol aqueous solution, and stirring for 20min at the stirring speed of 60 r/min; stirring for 4min at a stirring speed of 2000r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

3) continuously adding 5 parts of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 5 parts of deep-sea fish skin collagen into the first mixed solution, stirring for 15min at the stirring speed of 30r/min, and uniformly stirring to obtain a second mixed solution;

4) and continuously adding 0.005 part of carnosine, 10 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20 parts of acetyl tetrapeptide and 90.0005 parts of hexapeptide into the second mixed solution, stirring the mixture uniformly at the stirring speed of 30r/min, standing the mixture for 1.5 hours, filtering the mixture by adopting a 800-mesh filter screen and a layer plate filter, and checking the mixture to be qualified to obtain the compound anti-aging skin-firming polypeptide skin care product.

Example 2

The compound anti-aging firming polypeptide skin care product provided by the embodiment 2 specifically comprises the following raw materials in parts by weight:

0.005 part of carnosine, 0. 10.0005 part of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 0 part of swertia extract, 0 part of beta-glucan, 0 part of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep-sea fish skin collagen, 110 parts of biogum glycocoll, 48 parts of biogum glycocoll and 65.693 parts of water.

The compound anti-aging skin-firming polypeptide skin care product provided by the embodiment 2 is obtained by the following method:

1) adding 65.693 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 8min at a stirring speed of 50 r/min; stirring for 4min at a stirring speed of 2000r/min to fully dissolve the mixture to obtain a 1, 3-butanediol aqueous solution;

2) continuously adding 0 part of swertia extract, 0 part of beta-glucan, 110 parts of biological carbohydrate gum and 48 parts of biological carbohydrate gum into the 1, 3-butanediol aqueous solution, and stirring for 20min at the stirring speed of 60 r/min; stirring for 4min at a stirring speed of 2000r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

3) continuously adding 0 part of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 5 parts of deep-sea fish skin collagen into the first mixed solution, stirring for 15min at the stirring speed of 30r/min, and uniformly stirring to obtain a second mixed solution;

4) and continuously adding 0.005 part of carnosine, 10.0005 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide and 90.0005 parts of hexapeptide into the second mixed solution, stirring the mixture uniformly at the stirring speed of 30r/min, standing the mixture for 1.5 hours, filtering the mixture by adopting a 800-mesh filter screen and a layer plate filter, and checking the mixture to be qualified to obtain the compound anti-aging and skin-firming polypeptide skin care product.

Example 3

The compound anti-aging firming polypeptide skin care product provided by the embodiment 3 specifically comprises the following raw materials in parts by weight:

0.005 part of carnosine, 0.005 part of tripeptide-10.0005 part, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 0 part of deep-sea fish skin collagen, 10 parts of biogum, 40 parts of biogum and 75.693 parts of water.

The compound anti-aging skin-firming polypeptide skin care product provided by the embodiment 3 is obtained by the following method:

1) adding 75.693 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 8min at a stirring speed of 50 r/min; stirring for 4min at a stirring speed of 2000r/min to fully dissolve the mixture to obtain a 1, 3-butanediol aqueous solution;

2) continuously adding 3 parts of swertia extract, 5 parts of beta-glucan, 10 parts of biological carbohydrate gum and 40 parts of biological carbohydrate gum into the 1, 3-butanediol aqueous solution, and stirring for 20min at the stirring speed of 60 r/min; stirring for 4min at a stirring speed of 2000r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

3) continuously adding 5 parts of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 0 part of deep-sea fish skin collagen into the first mixed solution, stirring for 15min at the stirring speed of 30r/min, and uniformly stirring to obtain a second mixed solution;

Example 4

The compound anti-aging firming polypeptide skin care product provided by the embodiment 4 specifically comprises the following raw materials in parts by weight:

0.005 part of carnosine, 10.0005 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep-sea fish skin collagen, 110 parts of biogum glycocoll, 48 parts of biogum glycocoll and 52.693 parts of water.

The compound anti-aging skin-firming polypeptide skin care product provided by the embodiment 4 is obtained by the following method:

1) adding 52.693 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 8min at a stirring speed of 50 r/min; stirring for 4min at a stirring speed of 2000r/min to fully dissolve the mixture to obtain a 1, 3-butanediol aqueous solution;

2) continuously adding 3 parts of swertia extract, 5 parts of beta-glucan, 110 parts of biological carbohydrate gum and 48 parts of biological carbohydrate gum into the 1, 3-butanediol aqueous solution, and stirring for 20min at the stirring speed of 60 r/min; stirring for 4min at a stirring speed of 2000r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

Example 5

The compound anti-aging skin-firming polypeptide skin care product provided in this example 5 is the same as the raw materials and the parts by weight described in example 4.

The compound anti-aging skin-firming polypeptide skin care product provided in the embodiment 5 is obtained by the following method:

1) adding 52.693 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 5min at a stirring speed of 60 r/min; stirring for 3min at the stirring speed of 2500r/min to fully dissolve the mixture to obtain 1, 3-butanediol aqueous solution;

2) continuously adding 3 parts of swertia extract, 5 parts of beta-glucan, 110 parts of biological carbohydrate gum and 48 parts of biological carbohydrate gum into the 1, 3-butanediol aqueous solution, and stirring for 10min at the stirring speed of 80 r/min; stirring for 3min at the stirring speed of 2500r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

3) continuously adding 5 parts of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 5 parts of deep-sea fish skin collagen into the first mixed solution, stirring for 10min at the stirring speed of 45r/min, and uniformly stirring to obtain a second mixed solution;

4) and continuously adding 0.005 part of carnosine, 10.0005 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide and 90.0005 parts of hexapeptide into the second mixed solution, stirring the mixture uniformly at the stirring speed of 45r/min, standing the mixture for 1h, filtering the mixture by adopting a 500-mesh filter screen and a laminate filter, and checking the mixture to be qualified to obtain the compound anti-aging skin-firming polypeptide skin care product.

Example 6

The compound anti-aging skin-firming polypeptide skin care product provided in this embodiment 6 is the same as the raw materials and the parts by weight described in embodiment 4.

The compound anti-aging skin-firming polypeptide skin care product provided in the embodiment 6 is obtained by the following method:

1) 52.693 parts of water and 10 parts of 1, 3-butanediol are added into the sterilized emulsifying pot, and the mixture is stirred for 5min under the condition that the stirring speed is 55 r/min; stirring for 5min at the stirring speed of 2500r/min to fully dissolve the mixture to obtain 1, 3-butanediol aqueous solution;

2) continuously adding 3 parts of swertia extract, 5 parts of beta-glucan, 110 parts of biological carbohydrate gum and 48 parts of biological carbohydrate gum into the 1, 3-butanediol aqueous solution, and stirring for 30min at the stirring speed of 70 r/min; stirring for 5min at the stirring speed of 2500r/min to fully stir and dissolve the mixture to obtain a first mixed solution;

3) continuously adding 5 parts of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 5 parts of deep-sea fish skin collagen into the first mixed solution, stirring for 20min at the stirring speed of 40r/min, and uniformly stirring to obtain a second mixed solution;

4) and continuously adding 0.005 part of carnosine, 10.0005 parts of tripeptide, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide and 90.0005 parts of hexapeptide into the second mixed solution, stirring the mixture uniformly at the stirring speed of 40r/min, standing the mixture for 2 hours, filtering the mixture by adopting a 1000-mesh filter screen and a laminate filter, and checking the mixture to be qualified to obtain the compound anti-aging skin-firming polypeptide skin care product.

Comparative example the compound anti-aging firming polypeptide skin care product provided by the comparative example specifically comprises the following raw materials in parts by weight:

0 part of carnosine, 10 parts of tripeptide, 90 parts of acetyl tetrapeptide, 20 parts of acetyl tetrapeptide, 90 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep sea fish skin collagen, 110 parts of biogum glycocoll, 48 parts of biogum glycocoll and 52.7 parts of water.

The compound anti-aging firming polypeptide skin care product provided by the comparative example is obtained by the following method:

1) adding 52.7 parts of water and 10 parts of 1, 3-butanediol into the sterilized emulsifying pot, and stirring for 8min at the stirring speed of 50 r/min; stirring for 4min at a stirring speed of 2000r/min to fully dissolve the mixture to obtain a 1, 3-butanediol aqueous solution;

3) and continuously adding 5 parts of recombinant collagen, 0.8 part of 1, 2-hexanediol, 0.5 part of jasminolone and 5 parts of deep sea fish skin collagen into the first mixed solution, stirring for 15min at the stirring speed of 30r/min, standing for 1.5h after stirring uniformly, filtering by adopting a 800-mesh filter screen and a layer plate filter, and inspecting to be qualified to obtain the compound anti-aging skin-firming polypeptide skin care product.

The following table 1 shows the ratio of each raw material component in examples 1 to 4 and comparative example:

in order to verify the anti-wrinkle effect of the compound anti-aging skin-firming polypeptide skin care product obtained by the invention, the skin care products obtained in the embodiments 1 to 6 and the comparative example are subjected to an anti-wrinkle capability test, which specifically comprises a facial static line clearing capability test, an elastic fiber tissue test and a skin moisture loss test.

1. Facial static line removal capability detection

The detection is carried out by adopting a VISIA professional skin image analysis system, and the specific detection method comprises the following steps:

the VISIA skin detector is currently the only instrument that can quantify skin pathology. The two international authoritative skin databases of the Proter and the Gamble provide support, and a large amount of clinical first-hand data realizes international transition in the aspect of diagnosis and treatment of problem skins, and is more authoritative and professional. The VISIA skin problem detector can detect the problems exposed on the surface of the skin, can also visually display the problems hidden in the basal layer of the skin in front of a user through quantitative analysis, so that the user can have sufficient time to put the problems out in a sprouting state, and the VISIA skin problem detector can detect six skin health indexes at one time.

The principle of action of the VISIA skin detector is as follows: the VISIA skin detector is currently the only instrument that can quantify skin pathology. VISIA skin monitors use advanced optical imaging, RBX and software technology to detect and analyze skin blemishes, pores, wrinkles and skin texture, as well as subcutaneous vascular and pigmented lesions such as porphyrins (lipids), brown spots, erythema, etc. in real time as well as the potential risk of developing them such as chloasma, acne, rosacea and spider's vein tumors. Thereby enabling the dermatologist to design the most appropriate treatment for the skin problem. The VISIA skin detector can detect the problems exposed on the skin surface, and can also visually display the problems hidden in the skin basal layer in front of a user through quantitative analysis, so that the user can have sufficient time to put out the problems in a bud state.

The VISIA skin detector has the specific functions of: 1) quantitative analysis of skin characteristics: detecting and analyzing color deposition points, pores, skin color uniformity, wrinkles and UV (ultraviolet) reflection points; 2) grouping for skin conditions: comparing the skin characteristics of the female patient against skin types of other individuals of the same age; 3) cyclic facial photography: the cyclic shooting of the image between the secondary point and the built-in determined position under the standard illumination is ensured; 4) multispectral imaging: surface and subsurface skin condition indications were recorded and measured using standard and uv photography: spots, pore condition, fine lines in the skin, evenness of skin tone, facial infection, and uv spots in the basal layer of the skin.

Canfield, usa has recently developed a method for diagnosing skin with accurate quantification-this is the latest Visia skin diagnosis technology. The appearance of the system makes skin treatment distinguish the history of the past judgment by the experience of doctors only by naked eyes, can diagnose the skin condition accurately and quantitatively, provides more accurate, clear and easily understood skin diagnosis reports for doctors and patients, thereby performing skin treatment more effectively, improving treatment effect, and can quantitatively evaluate skin color spots, pores, wrinkles, flatness, porphyrin, ultraviolet spots and sunlight damage. It can help you know several problems with skin aging: skin pigmentation spots, blood circulation distribution, sun damage, pores, wrinkles, and can be used to track treatment progress, even with minimal changes, and quantitatively display the confidence and direction of treatment; second, a comparative ranking of skin characteristics was performed with the population under the same conditions (same age, gender, and skin type).

1) The inline position and standard illumination ensure consistent and repeatable conditions for each image.

2) Various spectral images: the skin surface and subcutaneous conditions were measured using standard white light, cross-sectional polarized flash light and ultraviolet light as multispectral photography.

3) RBX techniques the new RBX technique provides an unusual and novel method of exploring, visualizing and analyzing subcutaneous pigment and vascular conditions.

The specific detection data results are shown in Table 2 below

TABLE 2 comparison of facial static streak detection data

	Example 1	Example 2	Example 3	Example 4	Comparative example
						Number of static lines	22	20	18	8	30
Static streak clearance/%)	56	60	64	84	40
						Peak to valley texture	16.2	13.4	12.6	8.7	21.5

Through detection of a VISIA skin detector from three directions of static line quantity, static line clearance rate and line peak-valley value, detection data show that the compound anti-aging skin-firming polypeptide skin care product obtained in example 4 (carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2, hexapeptide-9, swertia extract, recombinant collagen, beta-glucan, deep-sea fish skin collagen, bioglycan-1 and bioglycan-4) can achieve the best and most ideal effect.

2 elastic fibrous texture test

The CK skin tester is used for detection, and the testing principle is based on resonance sound wave propagation time measurement. During measurement, two needle-shaped sensors inside the probe are placed on the surface of the skin to be measured, one sensor emits sound shock waves, and the other sensor receives the sound shock waves. The propagation speed and the propagation time of the acoustic shock wave are different due to the difference in moisture and elasticity of the skin surface. This allows the propagation time of the acoustic wave to be measured under different skin moisture and elasticity conditions. The better the elasticity and the less wrinkled skin, the shorter its propagation time. Conversely, the less elastic and wrinkled skin has a longer propagation time. The fiber tissue direction of the skin can be judged by testing in different directions at the same skin position.

The specific detection data results are shown in Table 3 below

TABLE 3 comparison of elastic fibrous tissue test data

	Example 1	Example 2	Example 3	Example 4	Comparative example
						Amount of skin stretching	2.4	1.8	1.4	0.8	5.4

The skin after the skin care products obtained by the four groups of examples and the comparative example are respectively detected by a CK skin tester, and the detection data show that: the compound anti-aging skin-firming polypeptide skin care product obtained in example 4 (carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2, hexapeptide-9, swertia extract, recombinant collagen, beta-glucan, deep-sea fish skin collagen, biosaccharin-1 and biosaccharin-4) has the shortest time, and shows that the skin elasticity is the best.

Skin Water loss test

The TEWL is a very important parameter for evaluating the function of the skin moisture barrier (i.e. the importance of the skin moisture loss test) and has been widely accepted internationally. The more intact the skin protection layer, the higher the moisture content and the lower the value of TEWL for skin moisture loss, in units of: g/hm². In the process of developing cosmetics, the efficacy of moisturizing cosmetics can be evaluated by testing the value of TEWL (water loss in skin), and the method can also be applied to allergic patch test, contact dermatitis, physical therapy, burn and monitoring of new tissues, andit is found whether the protective function of the skin has been destroyed. The TEWL tester, Tewameter (TM) 300, is a very effective instrument for measuring and evaluating the efficacy of cosmetics, nutraceuticals and drugs on the skin.

The specific detection data results are shown in Table 4 below

Table 4 comparison of skin moisture loss test data

Composition of	Example 1	Example 2	Example 3	Example 4	Comparative example
						TEWL value	40.8	31.5	20.3	12.8	66.2

Through the detection of the TEWL values of the skin moisture loss after the skin care products obtained by four groups of examples and a group of comparative examples are respectively used, the TEWL value of the compound anti-aging skin-firming polypeptide skin care product obtained by example 4 (carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2, hexapeptide-9, swertia extract, recombinant collagen, beta-glucan, deep-sea fish skin collagen, bioglycan-1 and bioglycan-4) is detected to be minimum, and the skin moisture loss is minimum, and the skin plumpness is optimal.

In conclusion, the compound anti-aging skin-firming polypeptide skin care product obtained by selecting the components can effectively adjust the number of static lines on the face, improve the synthesis of collagen, increase the skin elasticity, greatly improve the skin surface glossiness, make the skin color uniform, improve the yellowing, and have certain functions of brightening the skin color, tendering the skin and removing wrinkles.

In addition, the five polypeptide substances in the technical scheme of the invention have mature preparation process, mature technology, stable quality and high purity of effective active substances, and the precise synthesis technology and enzyme digestion technology are utilized to ensure that the purity of active ingredients is improved, so that the polypeptide substances are quickly dissolved out into a solvent, the extraction operation is quickly completed, and the interference of excessive factors such as temperature, pH, pressure and the like on the active substances is avoided; the swertiamarin is easy to absorb through the skin surface, is decomposed after absorption to generate erythrothecaurin, can expand capillary vessels, activates or promotes an enzyme system of skin cells, and improves the biochemical function of skin tissues. Can raise skin temperature and promote skin oxidation-reduction function. The beta-glucan has the effects of resisting aging, inhibiting inflammatory reaction of skin, moisturizing, removing wrinkles, resisting allergy and repairing skin.

In addition, aiming at the functions of anti-aging and wrinkle removal: the beta-glucan has the effects of improving the activity of skin cells and promoting the metabolism of the skin and also has strong antioxidation, thereby being capable of well delaying the aging; aiming at anti-allergy, anti-inflammation and skin function restoration: the beta-glucan can effectively improve the skin immunity and accelerate the healing of wounds, thereby realizing the anti-allergy and anti-inflammation effects; aiming at the moisturizing effect: the beta-glucan has strong moisturizing effect, can form a transparent, elastic and breathable film on the surface of the skin, and locks the moisture of the skin to play a role in high moisturizing. Can effectively improve the anti-wrinkle and wrinkle-smoothing effects of the polypeptide substances, keep the cells alive for a long time and delay aging.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. The compound anti-aging firming polypeptide skin care product is characterized by comprising the following raw materials in parts by weight:

2. The compound anti-aging firming polypeptide skin care product according to claim 1, which is characterized by comprising the following raw materials in parts by weight:

3. The compound anti-aging firming polypeptide skin care product according to claim 2, which is characterized by comprising the following raw materials in parts by weight:

0.005 part of carnosine, 0.005 part of tripeptide-10.0005 part, 90.0005 parts of acetyl tetrapeptide, 20.0005 parts of acetyl tetrapeptide, 90.0005 parts of hexapeptide, 3 parts of swertia extract, 5 parts of beta-glucan, 5 parts of recombinant collagen, 10 parts of 1, 3-butanediol, 0.8 part of 1, 2-hexanediol, 0.5 part of carissone, 5 parts of deep sea fish skin collagen, 110 parts of biogum, 48 parts of biogum and 52.693 parts of water.

4. The compound anti-aging skin-firming polypeptide skin care product according to any one of claims 1-3, wherein the purity of carnosine, tripeptide-1, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and hexapeptide is more than 95%.

5. A preparation method of the compound anti-aging skin-firming polypeptide skin care product as claimed in any one of claims 1-4, which is realized by the following steps:

6. The preparation method of the compound anti-aging skin-firming polypeptide skin care product according to claim 5, wherein the specific stirring method in the S2 is as follows: firstly, stirring for 5-10 min under the condition that the stirring speed is 50-60 r/min; and then stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min.

7. The preparation method of the compound anti-aging skin-firming polypeptide skin care product according to claim 6, wherein the specific method for stirring in S3 is as follows: firstly, stirring for 10-30 min under the condition that the stirring speed is 60-80 r/min; and then stirring for 3-5 min under the condition that the stirring speed is 2000-2500 r/min.

8. The preparation method of the compound anti-aging skin-firming polypeptide skin care product according to claim 7, wherein the specific stirring method in the S4 is as follows: stirring for 10-20 min under the condition that the stirring speed is 30-45 r/min.

9. The preparation method of the compound anti-aging skin-firming polypeptide skin care product according to any one of claims 5 to 8, wherein the specific stirring method in S5 is as follows: stirring the mixture evenly at a stirring speed of 30-45 r/min.

10. The method for preparing the compound anti-aging skin-firming polypeptide skin care product according to claim 9, wherein in S5, the standing time is 1-2 hours; and during filtering, a 500-1000 mesh filter screen and a laminate filter are adopted for filtering.