CN112915060A - Emulsion containing anti-aging composition and preparation method thereof - Google Patents
Emulsion containing anti-aging composition and preparation method thereof Download PDFInfo
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- CN112915060A CN112915060A CN202110418721.4A CN202110418721A CN112915060A CN 112915060 A CN112915060 A CN 112915060A CN 202110418721 A CN202110418721 A CN 202110418721A CN 112915060 A CN112915060 A CN 112915060A
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- aging composition
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Abstract
The invention provides an emulsion containing an anti-aging composition and a preparation method thereof. The preparation raw materials of the emulsion containing the anti-aging composition comprise: anti-aging compositions, skin conditioners, moisturizers and water; the anti-premature composition comprises: ginger root extract, scutellaria root extract and cacao seed extract; the skin conditioning agent comprises: hydrolyzed red algae extract, ginseng extract, and edelweiss extract. The anti-aging composition can be matched with each component in a skin conditioner of emulsion, has synergistic effect, can better permeate into skin, helps to decompose and metabolize impurities, prevents pigmentation, and makes the skin smooth, delicate, fair and glossy after use; and the skin can be restored to the optimal physiological state by adjusting the physiological function of the skin aiming at the sensitive skin, and the skin repairing and relieving effects are very obvious.
Description
Technical Field
The invention belongs to the technical field of cosmetic production and application, and particularly relates to an emulsion containing an anti-aging composition and a preparation method thereof.
Background
The skin of a human body gradually enters a natural aging state from about 25 years old, the aging speed shows a certain difference due to different genetic genes and living habits, and the early-aged manifestation is not obvious at this moment and reaches the early end of the age of about 30 years old. In addition to the multiple stresses and environmental pollution from modern society, the primary signs of early age are: the appearance of fine lines, superficial wrinkles and dry lines, obvious skin saccharification phenomenon, yellowing and roughness, pigmentation, dullness and darkness; the moisture content of the horny layer is reduced, and the skin is dry or pores are large and emit oil; the skin defense ability and blood circulation ability are reduced, and the skin is sensitive. The onset of aging manifests itself as a general decline in the condition of the skin, which is not completely the same as the signs of aging, such as wrinkling and atrophy, and requires relief from multiple skin care dimensions. The demand of people for products for resisting the premature aging is increasing day by day, and the products for resisting the premature aging must be capable of effectively preventing and delaying the skin aging caused by internal and external causes. The main effects of the skin care products on the market at present are mainly moisturizing, the effects are single, and the skin care products are weak in the aspect of resisting the premature senility.
Because the skin care products aiming at improving the comprehensive state of the skin are relatively less or have poor using effect or large irritation. Poor effect or irritation is closely related to compatibility of the formula. And along with climate change, air pollution and complexity of cosmetic types and components, the generation of sensitive skin is improved, and the perception rate and attention of sensitive skin are gradually increased due to improvement of people's consciousness on skin health. Developers in the cosmetics industry are aware of this phenomenon, placing products directed to sensitive skin in an increasingly important position, and have developed a large number of actives directed to various causes and manifestations of sensitive skin.
CN111450010A discloses a composition with anti-aging effect and application thereof in cosmetics. The composition comprises encapsulated retinol, retinol palmitate, carnosine, ascorbyl glucoside, niacinamide, citrus peel extract, hydrolyzed sodium hyaluronate, sodium hyaluronate cross-linked polymer, beta-oat glucan, and Aesculus hippocastanum seed extract. The cosmetic provided by the invention contains the composition with the anti-aging effect, has the effects of improving and improving the comprehensive state of skin, and is beneficial to recovering the health state of the skin and resisting the skin aging.
CN111973508A discloses a soothing and moisturizing anti-aging composition, which comprises the following components in percentage by weight: 0.02-0.2% of dipotassium glycyrrhizinate, 1-3% of chamomile extract, 1-15% of anti-aging composition, 1-20% of humectant, 0.05-1% of thickening agent, 0.5-2.5% of emulsifier, 1-10% of emollient and the balance of pure water; wherein the anti-aging composition is acetyl hexapeptide-8, a split yeast fermentation product lysate, a tea extract, a grape seed extract, and a blackberry leaf extract; the composition disclosed by the invention has the advantages that through reasonable matching of various components, the damage to the skin caused by various light sources is relieved and repaired, deep water is provided for the skin, and the effect of resisting aging in time is achieved.
Therefore, the development of a skin care product with soothing and skin-repairing functions is the focus of research in the field.
Disclosure of Invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide an emulsion containing an anti-aging composition and a method for preparing the same. The emulsion containing the anti-aging composition has the effects of long-acting relieving and skin repairing.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an emulsion comprising an anti-premature composition, the emulsion comprising the anti-premature composition prepared from: anti-aging compositions, skin conditioners, moisturizers and water;
the anti-premature composition comprises: ginger root extract, scutellaria root extract and cacao seed extract;
the skin conditioning agent comprises: hydrolyzed red algae extract, ginseng extract, and edelweiss extract.
In the invention, the anti-aging composition improves the tolerance of the skin by regulating the redox balance of cells, regulating cell autophagy and promoting cell detoxification, and can be matched with each component in the skin conditioner of the emulsion to realize synergistic interaction, thereby improving the effects of relieving and repairing the emulsion.
Wherein, the ginger flower root extract can regulate autophagy of fibroblast, thereby realizing metabolism requirement of cells, update of certain organelles and maintain cell homeostasis; even under the blue light pressure, the lysosome network of the fibroblast can be protected, the skin is helped to improve the natural protective ability of the skin, so that the blue light/UV injury can be resisted, the cell detoxification can be promoted, the skin regeneration can be promoted, and the like. The radix Scutellariae extract has effects of inhibiting elastase, interfering secretion, and scavenging free radicals, thereby increasing skin metabolism, skin elasticity and wrinkle resistance, and resisting aging; promoting cerebral amide, improving sebum, and moistening skin; has effects in inhibiting dermatitis, enhancing skin resistance, and resisting inflammation; can neutralize released histamine, and has effect in preventing allergy. The cocoa seed extract is rich in molecular peptide, saccharide and polyphenol, reduces active oxygen under the blue light pressure, protects opsin photoreceptor under the blue light pressure, increases the content of collagen I, fibrillarin-1 and syndecan-4, and obviously improves the fiber network structure of elastin, thereby improving the skin elasticity and obviously improving the skin wrinkles.
The hydrolyzed red algae extract can regulate neurogenic inflammation, is synergistic with an anti-aging composition for inhibiting chronic inflammation, can effectively inhibit skin redness, reduce skin pruritus and has the effect of relieving the skin. The Ginseng radix extract has effects of delaying skin aging, preventing skin dryness and dehydration, increasing skin elasticity, protecting skin luster and tenderness, preventing and reducing skin wrinkle, and inhibiting melanin reduction, and whitening and smoothing skin. The alpine edelweiss extract contains rich mineral substances, has the effects of relieving, calming, whitening and nourishing and protecting the skin, can mildly and deeply clean the skin, remove the grease of the epidermis and the dirt in pores, relieve the skin tension state, is soft and not tight, has natural whitening components, can permeate into the skin, helps to decompose and metabolize impurities, prevents pigmentation, and makes the skin smooth, fine, fair and glossy after use.
Preferably, in the anti-aging composition, the mass ratio of the ginger flower root extract, the scutellaria root extract and the cacao seed extract is (3-8): (5-15): (2-7);
wherein "3-8" can be 3, 4, 5, 6, 7, 8, etc.;
wherein, 5-15 can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, etc.;
wherein "2-7" may be 2, 3, 4, 5, 6, 7, etc.
Preferably, in the skin conditioner, the mass ratio of the hydrolyzed red algae extract, the ginseng extract and the edelweiss extract is (1-5): (0.1-0.5);
wherein "1-5" can be 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, etc.;
wherein the first "0.1-0.5" can be 0.1, 0.2, 0.3, 0.4, 0.5, etc.;
wherein the second "0.1-0.5" can be 0.1, 0.2, 0.3, 0.4, 0.5, etc.
Preferably, the raw materials for preparing the emulsion containing the anti-aging composition comprise the following components in percentage by mass, based on 100% by mass of the raw materials for preparing the emulsion containing the anti-aging composition: 0.01-5% of anti-aging composition, 0.01-10% of skin conditioner, 1-2% of emulsifier, 5-25% of humectant and the balance of water.
The content of the anti-premature composition is 0.01 to 5%, and may be, for example, 0.01%, 0.02%, 0.05%, 0.1%, 0.15%, 0.2%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%, or the like.
The content of the skin conditioner is 0.01 to 10%, and may be, for example, 0.01%, 0.02%, 0.05%, 0.1%, 0.15%, 0.2%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or the like.
The content of the emulsifier is 1 to 2%, and may be, for example, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, or the like.
The content of the humectant may be 5 to 25%, for example, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 22%, 25%, or the like.
Preferably, the skin conditioning agent further comprises: one or more of radix Sophorae Flavescentis extract, radix Glycyrrhizae Uralensis extract, nicotinamide, yeast/fructus oryzae fermentation product filtrate, cracked yeast fermentation product lysate, animal umbilical cord extract, animal placenta protein or animal placenta enzyme.
Preferably, the skin conditioning agent comprises, based on 100% by mass of the starting materials for the preparation of the emulsion containing the anti-aging composition: 1-5% of hydrolyzed red algae extract, 0.1-0.5% of ginseng extract, 0.1-0.5% of alpine edelweiss extract, 0.01-0.1% of sophora flavescens root extract, 0.01-0.1% of glycyrrhiza inflate root extract, 0.1-1% of nicotinamide, 0.01-0.1% of yeast/Szechwan grain seed fermentation product filtrate, 0.01-0.3% of schizosaccharomyces cerevisiae fermentation product lysate, 0.01-0.1% of animal umbilical cord extract, 0.01-0.1% of animal placental protein and 0.01-0.1% of animal placental enzyme.
Wherein the content of hydrolyzed red algae extract is 1-5%, such as 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%.
Wherein the content of Ginseng radix extract is 0.1-0.5%, such as 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%.
Wherein the content of the extract of Leontopodium alpinum is 0.1-0.5%, such as 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%.
Wherein the content of radix Sophorae Flavescentis extract is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.
Wherein the content of the extract of Glycyrrhiza inflata root is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.
The nicotinamide content is 0.1-1%, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, etc.
Wherein the content of yeast/Sichuan millet seed fermentation product filtrate is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.
The content of the secondary fission yeast fermentation product lysate may be, for example, 0.01 to 0.3%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%.
The content of the animal umbilical cord extract is 0.01-0.1%, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.
The content of animal placenta protein is 0.01-0.1%, such as 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%.
The content of animal placental enzyme is 0.01-0.1%, and may be, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, etc.
Preferably, the emulsifier comprises: c20-22 alcohol and/or C20-22 alcohol phosphate.
Preferably, the emulsifier comprises, based on 100% by mass of the raw materials for preparing the emulsion containing the anti-aging composition: 0.5-1% of C20-22 alcohol and 0.5-1% of C20-22 alcohol phosphate.
The content of the C20-22 alcohol is 0.5-1%, and may be, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or the like.
The content of C20-22 alcohol phosphate is 0.5-1%, and may be, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.
Preferably, the moisturizer comprises: any one or the combination of at least two of butanediol, glycerol polyether-26, trehalose, sodium hyaluronate or 1, 2-hexanediol.
Preferably, the humectant comprises, based on 100% by mass of a raw material for preparing the emulsion containing the anti-aging composition: 5-10% of butanediol, 262-5% of glycerol polyether, 2-4% of trehalose, 0.15-0.25% of sodium hyaluronate and 0.1-1% of 1, 2-hexanediol.
The content of butanediol is 5 to 10%, and may be, for example, 5%, 6%, 7%, 8%, 9%, 10%, or the like.
The content of the glyceryl polyether-26 is 2 to 5%, and may be, for example, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, or the like.
The trehalose content is 2 to 4%, and may be, for example, 2%, 2.5%, 3%, 3.5%, 4%, or the like.
The content of sodium hyaluronate is 0.15-0.25%, for example, 0.15%, 0.17%, 0.2%, 0.23%, 0.25%, etc.
The content of 1, 2-hexanediol is 0.1 to 1%, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, or the like.
Preferably, the preparation raw materials of the emulsion further comprise: any one or a combination of at least two of an emollient, a thickener, a pH adjuster, a chelating agent, an antioxidant, or a fragrance.
Preferably, the preparation raw materials of the emulsion containing the anti-aging composition further comprise, based on 100% by mass of the preparation raw materials of the emulsion: emollient 5-10%, thickener 0.2-0.5%, pH regulator 0.16-0.35%, chelating agent 0.01-0.05%, antioxidant 0.1-1%, and aromatic 0.01-0.05%.
The content of the emollient is 5 to 10%, and may be, for example, 5%, 5.5%, 6%, 6.5%, 7%, 8%, 9%, 10%, etc.
The content of the thickener is 0.2 to 0.5%, and may be, for example, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, or the like.
The content of the pH adjuster is 0.16 to 0.35%, and may be, for example, 0.16%, 0.18%, 0.2%, 0.22%, 0.24%, 0.26%, 0.28%, 0.3%, 0.32%, 0.35%, or the like.
The content of the chelating agent is 0.01 to 0.05%, and may be, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05% or the like.
The content of the antioxidant is 0.1 to 1%, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, or the like.
The content of the aromatic may be 0.01 to 0.05%, for example, 0.01%, 0.015%, 0.02%, 0.025%, 0.03%, 0.04%, 0.05%, etc.
Preferably, the emollient comprises any one or a combination of at least two of squalane, isononyl isononanoate, or jojoba oil.
Preferably, the thickener comprises an ammonium acryloyldimethyltaurate/VP copolymer and/or a carbomer.
Preferably, the pH adjusting agent is arginine.
Preferably, the chelating agent is disodium EDTA.
Preferably, the antioxidant is p-hydroxyacetophenone.
Preferably, the fragrance is a perfume.
In a second aspect, the present invention provides a process for the preparation of an emulsion comprising an anti-premature composition according to the first aspect, said emulsion comprising the steps of:
(1) mixing the humectant and water to obtain a water-phase mixed solution; mixing the emulsifier with an oil phase to obtain an oil phase mixed solution;
(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;
(3) and (3) mixing and stirring the homogenized liquid obtained in the step (2), the anti-aging composition and the skin conditioner to obtain the emulsion containing the anti-aging composition.
Preferably, the temperature of the aqueous phase mixing in step (1) is 80-85 ℃, for example 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃ and the like, and the time of the aqueous phase mixing is 5-10min, for example 5min, 6min, 7min, 8min, 9min, 10min and the like.
Preferably, the temperature of the oil phase mixing in step (1) is 75-80 deg.C, such as 75 deg.C, 76 deg.C, 77 deg.C, 78 deg.C, 79 deg.C, 80 deg.C, etc., and the time of the oil phase mixing is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.
Preferably, in step (1), the aqueous phase is mixed with a thickener.
Preferably, in step (1), the oil phase is mixed with an emollient.
Preferably, the temperature for mixing and homogenizing in step (2) is 80-82 deg.C, such as 80 deg.C, 80.5 deg.C, 81 deg.C, 81.5 deg.C, 82 deg.C, etc., and the time for mixing and homogenizing is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.
Preferably, the temperature of the mixing and stirring in the step (3) is 40-50 ℃, for example, 40 ℃, 42 ℃, 44 ℃, 46 ℃, 48 ℃, 50 ℃ and the like, and the time of the mixing and stirring is 5-10min, for example, 5min, 6min, 7min, 8min, 9min, 10min and the like.
Preferably, in the step (3), any one or a combination of at least two of a pH regulator, a chelating agent, an antioxidant or a fragrance is further added to the mixing and stirring.
Compared with the prior art, the invention has the following beneficial effects:
(1) the components in the anti-aging composition and the skin conditioner are synergistic, so that the fragile and sensitive state of the skin caused by the external environment can be effectively reduced, the resistance of the skin is enhanced, the elasticity of the skin is improved, wrinkles are reduced, the skin is reddened, the moisture of the skin is increased, and the skin is kept in a healthy state of gloss and fineness;
(2) the skin metabolism of the emulsion is regulated by various plant extracts, the skin can be permeated, impurities can be decomposed and metabolized, pigmentation can be prevented, the skin is smooth, delicate, fair and glossy after the emulsion is used, the skin can be repaired to reach the optimal physiological state by regulating the physiological function of the skin aiming at sensitive skin, and the emulsion has very obvious repairing and relieving effects.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Each of the components in the following preparation examples is commercially available.
Preparation example 1
The preparation example provides a curcuma alismatifolia root extract, which is prepared by the following preparation method:
(1) freezing root of Zingiber officinale Roscoe at-15 deg.C for 18h, pulverizing at-2 deg.C, and sieving to obtain 150 mesh Zingiber officinale Roscoe root powder;
(2) mixing the ginger flower root powder obtained in the step (1) with n-butanol, leaching for 2 hours at 25 ℃, and concentrating the solution obtained by leaching to obtain a concentrated solution; mixing the concentrated solution with ethanol, leaching at 40 deg.C for 1 hr, and filtering to obtain crude extract of rhizoma Zingiberis recens;
wherein the mass ratio of the ginger flower root powder to the n-butanol to the ethanol is 1:10: 8;
(3) and (3) passing the crude extract of the ginger flower roots obtained in the step (2) through macroporous adsorption resin AB-8, washing with deionized water, removing water solution, washing with 60 wt% ethanol water solution, collecting the washed ethanol water solution part, and concentrating to obtain the ginger flower root extract.
Preparation example 2
The preparation example provides a radix scutellariae extract, which is prepared by the following preparation method:
(a) pulverizing radix Scutellariae, and sieving to obtain 200 mesh radix Scutellariae powder;
(b) mixing the radix scutellariae powder obtained in the step (a) with an ethanol water solution with the concentration of 60 wt% according to the mass ratio of 1:10, performing microwave extraction for 8min at the power of 600W, and concentrating to obtain a radix scutellariae crude extract;
(c) dissolving the crude extract of radix scutellariae obtained in the step (b) by using 10 wt% of ethanol water solution, purifying by using a macroporous resin gel column D101, eluting by using 60 wt% of ethanol water solution, and concentrating to obtain the radix scutellariae extract.
Preparation example 3
The present preparation example provides a cocoa seed extract prepared by the following preparation method:
(A) pulverizing cacao seed to particle size of 100 mesh, mixing with n-hexane at a mass ratio of 1:10, soaking at 30 deg.C for 3 hr, filtering, and drying to obtain defatted cacao seed powder;
(B) mixing the defatted cocoa seed powder obtained in the step (A) with water, heating to 45 ℃, mixing with biological enzyme and acid, wherein the mass ratio of the defatted cocoa seed powder to the water to the cellulase is 1:15:0.006, carrying out enzymolysis reaction for 1.5h at 45 ℃ and pH of 5, and then inactivating to obtain a cocoa seed enzymolysis product;
(C) mixing the cocoa seed enzymolysis product obtained in the step (B) with 60 wt% ethanol water solution according to the mass ratio of 1:10, performing ultrasonic extraction for 50min at the power of 300W, filtering, and concentrating to obtain the crude extract of cocoa seeds.
Except that the ginger flower root extract, the scutellaria root extract and the cacao seed extract were prepared by the above-mentioned preparation methods, the remaining components in the following examples and comparative examples were all commercially available.
Example 1
This example provides an anti-aging composition comprising: 6 parts of ginger flower root extract, 10 parts of radix scutellariae extract and 4 parts of cocoa seed extract.
Example 2
This example provides an anti-aging composition, which comprises, in parts by weight: 5 parts of ginger flower root extract, 9 parts of radix scutellariae extract and 6 parts of cocoa seed extract.
Example 3
This example provides an anti-aging composition, which comprises, in parts by weight: 7 parts of ginger flower root extract, 8 parts of radix scutellariae extract and 5 parts of cocoa seed extract.
Example 4
This example provides an anti-aging composition, which comprises, in parts by weight: 3 parts of ginger flower root extract, 15 parts of radix scutellariae extract and 2 parts of cocoa seed extract.
Example 5
This example provides an anti-aging composition, which comprises, in parts by weight: 8 parts of ginger flower root extract, 5 parts of radix scutellariae extract and 7 parts of cocoa seed extract.
Comparative example 1
This comparative example provides a composition comprising, in parts by weight: 8 parts of ginger flower root extract and 12 parts of scutellaria root extract.
Comparative example 2
This comparative example provides a composition comprising, in parts by weight: 11 parts of ginger flower root extract and 9 parts of cocoa seed extract.
Comparative example 3
This comparative example provides a composition comprising, in parts by weight: 8 parts of radix scutellariae extract and 12 parts of cacao seed extract.
Application example 1
The application example provides an emulsion containing an anti-aging composition, and the preparation raw materials of the emulsion containing the anti-aging composition comprise the following components in percentage by mass:
the preparation method of the emulsion containing the anti-aging composition comprises the following steps:
(1) stirring and mixing the humectant, the thickener and water at 82 ℃ for 8min to obtain a water phase mixed solution; stirring and mixing the emollient and the emulsifier for 8min at 78 ℃ to obtain an oil phase mixed solution;
(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) at 80 ℃ and homogenizing for 8min to obtain homogenized liquor;
(3) mixing the homogenized solution obtained in step (2), anti-aging composition, skin conditioner, pH regulator, chelating agent, antioxidant and aromatic at 45 deg.C, and stirring for 6min to obtain the emulsion.
Application example 2
The application example provides an emulsion containing an anti-aging composition, and the preparation raw materials of the emulsion containing the anti-aging composition comprise the following components in percentage by mass:
the preparation method of the emulsion containing the anti-aging composition in the application example is the same as that in application example 1.
Application example 3
The application example provides an emulsion containing an anti-aging composition, and the preparation raw materials of the emulsion containing the anti-aging composition comprise the following components in percentage by mass:
the preparation method of the emulsion containing the anti-aging composition in the application example is the same as that in application example 1.
Application example 4
The application example provides an emulsion containing an anti-aging composition, and the preparation raw materials of the emulsion containing the anti-aging composition comprise the following components in percentage by mass:
the preparation method of the emulsion containing the anti-aging composition in the application example is the same as that in application example 1.
Application example 5
The application example provides an emulsion containing an anti-aging composition, and the preparation raw materials of the emulsion containing the anti-aging composition comprise the following components in percentage by mass:
the preparation method of the emulsion containing the anti-aging composition in the application example is the same as that in application example 1.
Application example 6
The application example provides an emulsion, and is different from the application example 1 in that trehalose is not added, the content of butanediol is increased to 6.0%, the content of glyceryl polyether-26 is increased to 4.0%, the content of sodium hyaluronate is increased to 1.2%, and the content of other components and the preparation method are the same as the application example 1.
Application example 7
The application example provides an emulsion, and is different from the application example 1 in that sodium hyaluronate is not added, the content of trehalose is increased to 3.2%, and the content of other components and the preparation method are the same as the application example 1.
Application example 8
The application example provides an emulsion, and is different from the application example 1 in that squalane is not added, the content of isononyl isononanoate is increased to 4.0%, the content of jojoba seed oil is increased to 2.5%, and the content of other components and the preparation method are the same as the application example 1.
Application example 9
The application example provides an emulsion, and is different from the application example 1 in that no jojoba seed oil is added, the squalane content is increased to 4.3%, the jojoba seed oil content is increased to 2.2%, and the content of other components and the preparation method are the same as those of the application example 1.
Comparative application example 1
This comparative application example provides an emulsion that differs from application example 1 in that the anti-aging composition provided in example 1 was replaced with the equal mass of the composition provided in comparative example 1, and the contents of other components and the preparation method were the same as in application example 1.
Comparative application example 2
This comparative application example provides an emulsion that differs from application example 1 in that the anti-aging composition provided in example 1 was replaced with the equal mass of the composition provided in comparative example 2, and the contents of other components and the preparation method were the same as in application example 1.
Comparative application example 3
This comparative application example provides an emulsion that differs from application example 1 in that the anti-aging composition provided in example 1 was replaced with the equal mass of the composition provided in comparative example 3, and the contents of other components and the preparation method were the same as in application example 1.
Comparative application example 4
The comparative application example provides an emulsion, which is different from the application example 1 in that the hydrolyzed red algae extract is not added, the content of the ginseng extract is increased to 1.2%, the content of the leontopodium alpinum extract is increased to 1.3%, and the content of other components and the preparation method are the same as the application example 1.
Comparative application example 5
The comparison application example provides an emulsion, which is different from the application example 1 in that no ginseng extract is added, the content of the hydrolyzed red algae extract is increased to 2.1%, the content of the alpine edelweiss extract is increased to 0.4%, and the content of other components and the preparation method are the same as the application example 1.
Comparative application example 6
The comparative application example provides an emulsion, which is different from the application example 1 in that the leontopodium alpinum extract is not added, the content of the hydrolyzed red algae extract is increased to 2.2%, the content of the ginseng extract is increased to 0.3%, and the content of other components and the preparation method are the same as the application example 1.
Test example 1
Safety performance testing
The safety performance tests were performed on the emulsions provided in application examples 1-9 and on the emulsions provided in comparative application examples 1-6 by the following method:
(1) haemolysis test of erythrocytes
Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Select samples were diluted with PBS solution to different concentrations, with 5 concentration gradients set for each sample. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing its absorbance at 560nm with spectrophotometer (A)560) Calculating the hemolysis rate according to the following formula;
a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated50)。
(2) Protein denaturation experiments:
diluting the sample to 10g/L with PBS solution, collecting 10mL dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing absorbance A at 540nm and 575nm with spectrophotometer540And A575Calculating a protein denaturation index (D) according to the following formula;
wherein R is1Blank control group a575Blank control group A540，R2Experimental group a575Experimental group A540，R3Positive control group A575Positive control group A540。
Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD50The following table 1 shows the experimental irritation grading criteria for erythrocyte hemolysis:
TABLE 1
| L/D | Grading |
| >100 | Has no irritation |
| 10<L/D≤100 | Micro-stimulation property |
| 1<L/D≤10 | Mild irritation |
| 0.1<L/D≤1 | Moderate irritation |
The results of the above-described hemolysis test and protein denaturation test are shown in Table 2 below:
TABLE 2
As can be seen from the safety performance test, the emulsion prepared by the application examples 1-9 of the invention is mild and non-irritant; the emulsion prepared by the invention has the sample concentration HD when 50% of red blood cells are subjected to hemolysis50Over 16000mg/L, HD greater than emulsions prepared in comparative application examples 1-650(ii) a Meanwhile, the protein denaturation index DI is below 2.5 percent and is also obviously smaller than the emulsion prepared by the comparative application examples 1-6, which shows that the addition of the anti-aging composition can obviously reduce the toxic and side effects and the irritation of the emulsion and is safer and more reliable.
Test example 2
Whitening efficacy test
The whitening efficacy tests were performed on the emulsions provided in application examples 1-9 and on the emulsions provided in comparative application examples 1-6 by the following method:
(1) tyrosinase activity inhibition assay
Mouse melanoma B16 cells were harvested from log phase growth and plated onto 6-well cell culture plates for overnight culture. The samples to be tested with a final volume fraction of 1% were added separately, and the untreated group was used as a cell control group, with 2 duplicate wells per group. After 48h of culture, the cells were washed 1 time with PBS, 100. mu.L of lysate was added to each well, the cells were scraped off and collected, and the supernatant was centrifuged. 50 μ L of cell supernatant was applied to a 96-well plate, 50 μ L of 1% L-dopa solution was added, incubation was performed at 37 ℃ for 1h, and absorbance was read at 475nm using M3 plate reader.
Relative tyrosinase activity (%) - (assay well absorbance-blank absorbance)/(cell control absorbance-blank absorbance) × 100%.
(2) Experiment for inhibiting melanin synthesis
Mouse melanoma B16 cells in logarithmic growth phase were inoculated into T25 cell culture flasks and cultured overnight. The samples to be tested were added to the test cells in a final volume fraction of 1%, and the untreated group was used as a cell control group. After 48h of culture, washing with PBS 1 time, adding 1mL of 1mol/L NaOH solution, scraping and collecting cells, placing in a water bath at 80 ℃ for 30min, adding the supernatant into a 96-well plate, and reading the absorbance value at 475nm by using an M3 plate reader.
Relative melanin content (%) — x 100% (assay well absorbance value-blank control absorbance value)/(cell control absorbance value-blank control absorbance value), wherein the tyrosinase activity of the control was 100% and the melanin content of the control was 100%.
(3) Human body evaluation of whitening effect
150 volunteers 25-50 years old are selected, male and female are not limited, the volunteers are randomly divided into 15 groups, 10 persons in each group are respectively smeared on the arm of each sample to be tested, the sample to be tested is respectively used for 1 time in the morning and at night every day, the smearing amount of each time is 2mL, and the total using time is 4 weeks. The skin melanin content change before and after use of the subject was evaluated using a skin red melanin tester (HexameterMX 18). The measurement range of the used instrument is 0-999, and the higher the measurement value, the higher the melanin content in the skin is.
The specific test results are shown in table 3:
TABLE 3
The test results in table 3 show that the relative tyrosinase activity of the emulsion provided by the invention is 17-18%, and the inhibition effect on tyrosinase is good; meanwhile, the synthesis of melanin in cells can be obviously inhibited, and the relative melanin content is 8-12%; and the human body evaluation result shows that the emulsion provided by the invention can obviously reduce the melanin content of the subject after being used for 4 weeks, and the reduction of the melanin of the subject reaches more than 60%. The anti-aging composition can be matched with each component in the skin conditioner of the emulsion to realize synergistic interaction, so that the anti-aging composition can effectively inhibit the synthesis and transfer of melanin, promote the decomposition and elimination of the melanin, inhibit the activity of tyrosinase, promote the metabolism of pigment, help the catabolic impurities, prevent pigmentation, and make the skin smooth, delicate, white and glossy after the composition is used.
Test example 3
Histamine inhibition test
Histamine inhibition tests were performed on the emulsions provided in application examples 1-9 and on the emulsions provided in comparative application examples 1-6 by the following method:
histamine is a chemical transmission medium in organisms, is present in cytoplasmic granules of basophils and mast cells of human bodies, and has positive correlation between skin allergy, inflammation and pruritus and histamine release in mast cells. Research proves that the anti-allergy and relieving effects can be achieved by inhibiting histamine release; experiments mast cell degranulation agent (Compound48/80) was used to induce histamine release from guinea pig isolated mast cells, which was then inhibited by the relaxation efficacy test using the emulsions provided in application examples 1-9 and the emulsions provided in comparative application examples 1-6. The content of histamine release from guinea pig mast cells was determined by HPLC, and the rate of inhibition of histamine release was measured for each sample.
The specific test results are shown in table 4:
TABLE 4
From the test results in table 4, it can be seen that the emulsion provided by the present invention has an inhibition rate of over 77% for histamine release. The anti-aging composition can be matched with each component in the skin conditioner of the emulsion, has synergistic effect, and can achieve the anti-allergy and relieving effects by inhibiting the release of histamine.
Test example 4
Functional assessment of soothing sensitive stimulus effects
(1) Test samples: the emulsions provided in application examples 1-9 and the emulsions provided in comparative application examples 1-6;
(2) the test method comprises the following steps: selecting 150 sensitive skin crowds (with the accumulated stabbing pain feeling more than or equal to 3 points) with the age of 25-50 years, randomly dividing the crowds into 15 groups of 10 people each; selecting a subject to perform a nasal sulcus test, wherein the tested part of the subject cannot use any cosmetics, external medicines or internal health care products 15 days before the test, and before the test, the subject is required to clean the face and sit still for 30min in a constant-temperature and constant-humidity room with the temperature of 22 +/-1 ℃ and the humidity of 50 +/-5% within 2 hours after the cleaning and is kept in a relaxed state;
dropping 50 μ L of 10% lactic acid solution on a single-layer filter paper with a diameter of 0.8c m, inquiring the testee about stabbing pain at 3min, 4min and 5min respectively, scoring according to a 4-point method (0 is no stabbing pain, 1 is mild stabbing pain, 2 is moderate stabbing pain and 3 is severe stabbing pain), recording the score of the testee as the initial value of the side lactic acid stimulation score (D0), carrying out the experiment for 7 days totally, using the product by the testee for 1 time each morning and evening according to the requirement, avoiding cross contamination, and carrying out the revisit by the testee on the 7 th day (D7); during testing, the lighting condition is kept consistent, the bed inclination angle and the bed placing position are one, the same testee is tested by the same measurer, and the change rate of the lactic acid stimulation result is calculated;
the specific test results are shown in table 5:
TABLE 5
The test data in table 5 show that the change rate of the lactic acid stimulation result reaches below-28% after 7 days of the emulsion provided by the invention, which indicates that the anti-aging composition provided by the invention can be matched with each component in the skin conditioner of the emulsion, has synergistic effect, can better repair the skin to reach the optimal physiological state by adjusting the physiological function of the skin aiming at the sensitive skin, has very obvious repairing and relieving effects on the sensitive skin, and is more consistent with the physiological requirements of the skin.
The applicant states that the invention is illustrated by the above examples of emulsions containing an anti-premature composition according to the invention and the process for their preparation, but the invention is not limited to the above examples, i.e. it is not meant to imply that the invention must be practiced in the light of the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. An emulsion containing an anti-aging composition, wherein the emulsion containing the anti-aging composition is prepared from the following raw materials: anti-aging compositions, skin conditioners, moisturizers and water;
the anti-premature composition comprises: ginger root extract, scutellaria root extract and cacao seed extract;
the skin conditioning agent comprises: hydrolyzed red algae extract, ginseng extract, and edelweiss extract.
2. The emulsion containing an anti-aging composition according to claim 1, wherein the anti-aging composition is characterized in that the mass ratio of the ginger flower root extract, the scutellaria root extract and the cacao seed extract is (3-8): (5-15): (2-7);
preferably, in the skin conditioner, the mass ratio of the hydrolyzed red algae extract, the ginseng extract and the edelweiss extract is (1-5): (0.1-0.5).
3. The emulsion containing the anti-aging composition according to claim 1 or 2, wherein the raw materials for preparing the emulsion containing the anti-aging composition comprise, based on 100% by mass of the raw materials for preparing the emulsion containing the anti-aging composition, by mass percent: 0.01-5% of anti-aging composition, 0.01-10% of skin conditioner, 1-2% of emulsifier, 5-25% of humectant and the balance of water.
4. An emulsion containing an anti-aging composition according to any of claims 1 to 3, characterized in that the skin conditioner further comprises: one or more of radix Sophorae Flavescentis extract, radix Glycyrrhizae Uralensis extract, nicotinamide, yeast/fructus oryzae fermentation product filtrate, cracked yeast fermentation product lysate, animal umbilical cord extract, animal placenta protein or animal placenta enzyme;
preferably, the skin conditioning agent comprises, based on 100% by mass of the starting materials for the preparation of the emulsion containing the anti-aging composition: 1-5% of hydrolyzed red algae extract, 0.1-0.5% of ginseng extract, 0.1-0.5% of alpine edelweiss extract, 0.01-0.1% of sophora flavescens root extract, 0.01-0.1% of glycyrrhiza inflate root extract, 0.1-1% of nicotinamide, 0.01-0.1% of yeast/Szechwan grain seed fermentation product filtrate, 0.01-0.3% of schizosaccharomyces cerevisiae fermentation product lysate, 0.01-0.1% of animal umbilical cord extract, 0.01-0.1% of animal placental protein and 0.01-0.1% of animal placental enzyme.
5. An emulsion containing an anti-aging composition according to any of claims 1 to 4, characterized in that the emulsifier comprises: c20-22 alcohol and/or C20-22 alcohol phosphate;
preferably, the emulsifier comprises, based on 100% by mass of the raw materials for preparing the emulsion containing the anti-aging composition: 0.5-1% of C20-22 alcohol and 0.5-1% of C20-22 alcohol phosphate.
6. The emulsion of any of claims 1-5, wherein the humectant comprises: any one or the combination of at least two of butanediol, glycerol polyether-26, trehalose, sodium hyaluronate or 1, 2-hexanediol;
preferably, the humectant comprises, based on 100% by mass of a raw material for preparing the emulsion containing the anti-aging composition: 5-10% of butanediol, 262-5% of glycerol polyether, 2-4% of trehalose, 0.15-0.25% of sodium hyaluronate and 0.1-1% of 1, 2-hexanediol.
7. The emulsion comprising an anti-aging composition according to any of claims 1 to 6, wherein the emulsion is prepared from the following raw materials: any one or a combination of at least two of an emollient, a thickener, a pH adjuster, a chelating agent, an antioxidant, or a fragrance;
preferably, the preparation raw materials of the emulsion containing the anti-aging composition further comprise, based on 100% by mass of the preparation raw materials of the emulsion: emollient 5-10%, thickener 0.2-0.5%, pH regulator 0.16-0.35%, chelating agent 0.01-0.05%, antioxidant 0.1-1%, and aromatic 0.01-0.05%;
preferably, the emollient comprises any one or combination of at least two of squalane, isononyl isononanoate, or jojoba oil;
preferably, the thickener comprises an ammonium acryloyldimethyltaurate/VP copolymer and/or a carbomer;
preferably, the pH adjusting agent is arginine;
preferably, the chelating agent is disodium EDTA;
preferably, the antioxidant is p-hydroxyacetophenone;
preferably, the fragrance is a perfume.
8. Method for the preparation of an emulsion containing an anti-aging composition according to any of claims 1 to 7, characterized in that the method for the preparation of the emulsion comprises the steps of:
(1) mixing the humectant and water to obtain a water-phase mixed solution; mixing the emulsifier with an oil phase to obtain an oil phase mixed solution;
(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;
(3) and (3) mixing and stirring the homogenized liquid obtained in the step (2), the anti-aging composition and the skin conditioner to obtain the emulsion containing the anti-aging composition.
9. The method for preparing an emulsion containing an anti-aging composition according to claim 8, wherein in the step (1), the temperature for mixing the aqueous phase is 80 to 85 ℃ and the time for mixing the aqueous phase is 5 to 10 min;
preferably, in the step (1), the temperature of the oil phase mixing is 75-80 ℃, and the time of the oil phase mixing is 5-10 min;
preferably, in step (1), the aqueous phase is mixed with a thickening agent;
preferably, in the step (1), an emollient is also added to the oil phase mixture;
preferably, in the step (2), the temperature for mixing and homogenizing is 80-82 ℃, and the time for mixing and homogenizing is 5-10 min.
10. The method for preparing an emulsion containing an anti-aging composition according to claim 8 or 9, wherein in the step (3), the temperature of the mixing and stirring is 40 to 50 ℃, and the time of the mixing and stirring is 5 to 10 min;
preferably, in the step (3), any one or a combination of at least two of a pH regulator, a chelating agent, an antioxidant or a fragrance is further added to the mixing and stirring.
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