CN113018202A - Dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as well as preparation method and application thereof - Google Patents
Dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as well as preparation method and application thereof Download PDFInfo
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- CN113018202A CN113018202A CN202110340217.7A CN202110340217A CN113018202A CN 113018202 A CN113018202 A CN 113018202A CN 202110340217 A CN202110340217 A CN 202110340217A CN 113018202 A CN113018202 A CN 113018202A
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- polysaccharide
- dendrobium officinale
- astragalus
- composite hydrogel
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as well as a preparation method and application thereof, wherein the prescription of the preparation takes the composite polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide as a main medicine; the preparation comprises the following components in percentage by mass: 1 to 2 percent of main medicine, 0.5 to 1.8 percent of gel matrix, 5 to 15 percent of skin penetration enhancer, 5 to 10 percent of humectant, 1 to 5 percent of antioxidant, 0.1 to 0.2 percent of preservative and the balance of water, and the pH value of the solution is adjusted by a pH regulator. The invention discloses a composite hydrogel, which is prepared by utilizing polysaccharides extracted from natural plants of dendrobium officinale and astragalus membranaceus and researching an external preparation of a skin care product for diminishing inflammation and resisting aging. The invention adopts the ball milling method for auxiliary extraction, retains the effective active ingredients of the dendrobium officinale to the maximum extent, reduces the cost, has simple method operation and is easy to realize industrial production.
Description
Technical Field
The invention relates to the technical field of daily skin care products, in particular to dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel and a preparation method and application thereof.
Background
Dendrobium officinale (Dendrobium officinale) is derived from various plants of the genus Dendrobium of the family Orchidaceae, is one of the largest genera of Orchidaceae, and is widely distributed in tropical and subtropical regions of Asia. China is mainly produced in Sichuan, Guizhou, Yunnan and Yangtze river basin and other places. The dendrobium officinale is a rare medicinal material in the traditional Chinese medicine, and has the effects of nourishing the stomach, promoting the production of body fluid, tonifying the liver, improving eyesight, tonifying the kidney, moistening the lung and strengthening the body. Researches show that the polysaccharide is an important component in dendrobium officinale, and the dendrobium officinale polysaccharide has wide biological activities of improving immunity, resisting oxidation, inflammation, tumor and the like. (Guo crown et al. study overview of pharmacological action of Dendrobii polysaccharide [ J ]. medical review, 2015,21(24):4525-4528.)
Astragalus membranaceus is the dried root of Astragalus membranaceus (Astragalus membranaceus) belonging to the family Leguminosae, and has the effects of tonifying qi, nourishing yang, benefiting lung, invigorating spleen, protecting liver, inducing diuresis, and the like, which are recorded in Shennong Ben Cao Jing. Radix astragali contains various active substances such as polysaccharide, protein, alkaloid, amino acid, flavone and trace elements. The astragalus polysaccharide is one of the main active ingredients of astragalus, can be used as an immunopotentiator to activate the immune system of animals, and has important effects in the aspects of resisting tumors, viruses and oxidation, controlling blood sugar, improving cardiovascular functions and the like. (splendid flare, Liu Shao Jing, Ma Xiu, Lu Rong, Wang Lin, Yang Min.) Astragalus polysaccharides pharmacological action and dosage form research progress [ J ] Guangzhou chemical industry, 2019,47(15):28-30.)
Gel cosmetics have been developed rapidly in recent years, and gel, a special external preparation, has many advantages, and more gel cosmetics in the medical field are made into pharmaceutical cosmetics, namely, cosmeceuticals. Chinese medicine resources are rich, varieties are numerous, purposes are wide, the development of natural plant cosmetics becomes a world trend at present, the effect is improved by adding Chinese medicines into the cosmetics, and the Chinese medicine cosmetic is the most promising approach for developing the cosmetics in China. The application of the gel technology can help to control the quality of the gel, thereby standardizing the development of the cosmeceutical.
Advantages of gel cosmetic: the gel cosmetic creatively adopts the gel as the using formulation of the cosmetic, has good absorption performance and is suitable for skin. The gel cosmetic can be added with transdermal absorption enhancer selectively to promote skin care preparation to absorb rapidly and completely, and exert effect rapidly. The preparation process of gel cosmetics develops rapidly in these years, and the types of cosmetics which can be contained are wide, such as crystals or powder of the cosmetics, fine powder or extract of traditional Chinese medicinal materials and the like. However, no composite gel preparation with good curative effect and small side effect is found in the market at present. Therefore, the invention adopts the ball milling method auxiliary technology to keep the effective components of the dendrobium officinale as much as possible, expands the application field of the dendrobium officinale and the astragalus membranaceus, gives full play to the efficacy of the dendrobium officinale and the astragalus membranaceus, and prepares the dendrobium officinale polysaccharide/astragalus membranaceus polysaccharide composite gel preparation with good curative effect.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as well as the preparation method and the application thereof.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the prescription of the preparation takes the composite polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide as a main medicine; the preparation comprises the following components in percentage by mass: 1 to 2 percent of main medicine, 0.5 to 1.8 percent of gel matrix, 5 to 15 percent of skin penetration enhancer, 5 to 10 percent of humectant, 1 to 2 percent of antioxidant, 0.1 to 0.2 percent of preservative and the balance of water, and the pH value is adjusted to be 6 by a pH regulator.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the gel matrix is at least one selected from acacia, gelatin, carbomer 980, sodium alginate, CMC-Na and HPMC, preferably carbomer 980.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the humectant is selected from at least one of 1, 2-propylene glycol and glycerol, and is preferably 1, 2-propylene glycol.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the skin-promoting penetrating agent is selected from diethylene glycol monoethyl ether.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the preservative is selected from at least one of methyl paraben, ethyl paraben and sodium benzoate, and preferably methyl paraben.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the antioxidant consists of vitamin C and vitamin E, and the mass ratio of the vitamin C to the vitamin E is 1: 1.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized in that the pH regulator is selected from sodium hydroxide or citric acid, and preferably sodium hydroxide.
The preparation method of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is characterized by comprising the following steps according to a composition formula:
1) drying the dendrobium officinale stems and the astragalus roots to constant weight, crushing, sieving, then placing the mixture into a planetary ball mill according to the mass ratio of 1:1, wherein the ball milling speed is 300rpm, the ball milling time is 5min, and the filling rate of the planetary ball mill is 20.9 percent calculated by the ratio of the total volume of the added stainless steel ball milling beads to the volume of a ball milling tank; finally, obtaining the milled dendrobium officinale/astragalus powder;
2) weighing and placing the ball-milled dendrobium officinale/astragalus membranaceus powder into a round-bottom flask, adding an extraction solvent water into the round-bottom flask, and extracting twice under the heating condition of 70 ℃, wherein the extraction time is 2 hours each time, the solid-liquid ratio in each extraction is 1:40, and the unit of the solid-liquid ratio is g/mL; mixing the two extracting solutions, carrying out rotary evaporation to obtain an extract, adding 95% ethanol according to a solid-to-liquid ratio of 1:40, wherein the solid-to-liquid ratio unit is g/mL, carrying out alcohol precipitation overnight, carrying out centrifugal filtration, and drying to obtain the compound polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide;
3) weighing a gel matrix, dissolving the gel matrix in part of purified water, adding a humectant, uniformly stirring, and swelling for 24 hours to obtain blank gel;
4) weighing the compound polysaccharide of the dendrobium officinale polysaccharide and the astragalus polysaccharide obtained in the step 2), dissolving the compound polysaccharide in part of purified water, adding the blank gel obtained in the step 3), and uniformly stirring;
5) weighing an antioxidant, a skin-promoting penetrating agent and a preservative, adding into the mixed solution obtained in the step 4), adjusting the pH to 6 with a pH regulator, supplementing the rest purified water, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel.
The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is applied to preparation of a medicine preparation or a beauty product preparation for relieving skin inflammation.
Compared with the prior art, the invention has the following advantages:
(1) the invention fully utilizes the dendrobium officinale and the astragalus, the polysaccharide in the dendrobium officinale has wide biological activities of improving immunity, resisting oxidation, resisting inflammation, resisting tumors and the like, and the astragalus polysaccharide is one of the main active ingredients of the astragalus, can be used as an immunopotentiator to activate the immune system of animals, and has important effects on the aspects of resisting tumors, resisting viruses, resisting oxidation, controlling blood sugar, improving cardiovascular functions and the like.
(2) The invention adopts a ball milling auxiliary extraction method, and reserves the dendrobium officinale polysaccharide to the maximum extent, thereby reducing the cost and saving precious resources. In the preparation product of the invention, the vitamin E and the vitamin C are used simultaneously, the two vitamins can generate synergistic interaction, and the vitamin C can recover the activity of the vitamin E to ensure that the vitamin E plays a role in scavenging free radicals.
(3) The raw materials of the invention are natural, no organic reagent is added in the extraction process, the gel formulation is natural and has no stimulation, the penetration of the effective components is faster, the skin absorption is better, and the skin quality can be effectively improved after long-term use.
Drawings
FIG. 1 is a change relationship of transdermal permeation amount of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel with time;
FIG. 2 is a pathological morphology diagram of auricle swelling tissues of each group of mice;
FIG. 3 is a comparison graph of DPPH free radical scavenging activity of Dendrobium officinale polysaccharide/Astragalus polysaccharides composite hydrogel, Dendrobium officinale polysaccharide hydrogel and vitamin C.
Detailed Description
The present invention is further illustrated by the following examples, which should not be construed as limiting the scope of the invention.
1. The invention relates to the following pharmaceutical specifications:
XS205 dual range analytical balance (Mettler Toledo, switzerland); PM200 planetary ball mill (Retsch, germany); KH5200DE ultrasonic cleaner (kunshan ultrasonic instruments ltd); heidolph magnetic stirring water bath; barnstead TII ultra pure water system (Thermo Scientific, USA) R-215 rotary evaporator (Buchi, Switzerland).
The water is ultrapure water (self-made in laboratories), the perfect aloe gel is purchased from perfect limited companies, the other reagents are of analytical grade, the sample dendrobium officinale medicinal material used in the experiments is purchased from Yunnan Tengchong, and the astragalus membranaceus medicinal material is purchased from Shaanxi Xian.
2. In the following embodiments, the preparation method of the compound polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide comprises the following steps:
1) placing the dendrobium officinale stems and the astragalus roots in a 60 ℃ oven to dry to constant weight, crushing, sieving by a third sieve, then placing in a planetary ball mill according to the mass ratio of 1:1, wherein the ball milling speed is 300rpm, the ball milling time is 5min, and the filling rate of the planetary ball mill is 20.9% calculated by the ratio of the total volume of the added stainless steel balls to the volume of a ball milling tank; finally, obtaining the milled dendrobium officinale/astragalus powder;
2) weighing and placing the ball-milled dendrobium officinale/astragalus membranaceus powder into a round-bottom flask, adding an extraction solvent water into the round-bottom flask, and extracting twice under the heating condition of 70 ℃, wherein the extraction time is 2 hours each time, the solid-liquid ratio in each extraction is 1:40, and the unit of the solid-liquid ratio is g/mL; and combining the two extracting solutions, performing rotary evaporation to obtain an extract, adding 95% ethanol according to a solid-to-liquid ratio of 1:40, wherein the solid-to-liquid ratio unit is g/mL, precipitating with ethanol overnight, centrifuging, filtering, and drying to obtain the compound polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide.
3. In the following embodiments, the preparation methods of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprise the following steps:
1) weighing a gel matrix, dissolving the gel matrix in part of purified water, adding a humectant, uniformly stirring, and swelling for 24 hours to obtain blank gel;
2) weighing compound polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide, dissolving the compound polysaccharide in part of purified water, adding the blank gel obtained in the step 1), and uniformly stirring;
3) weighing an antioxidant, a skin-promoting penetrating agent and a preservative, adding into the mixed solution obtained in the step 2), adjusting the pH to 6 with a pH regulator, supplementing the rest purified water, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel.
Example 1:
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 0.5g of carbomer 980 and 2.5g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 0.5g of ball-milled dendrobium officinale polysaccharide and astragalus polysaccharide composite powder, dissolving in purified water, uniformly stirring, adding into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 2.5g of diethylene glycol monoethyl ether, 50mg of methyl paraben, 0.5g of vitamin C and 0.5g of vitamin E, adding the mixture into the mixed solution obtained in the step 2), then adjusting the pH to about 5.0 by using 1% of sodium hydroxide solution, supplementing the remaining purified water until the total weight is 50g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
Example 2:
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 0.5g of carbomer 980 and 2.5g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 0.5g of the composite powder of the dendrobium officinale polysaccharide and the astragalus polysaccharide after ball milling, dissolving the composite powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 5g of diethylene glycol monoethyl ether, 75mg of methyl paraben, 0.5g of vitamin C and 0.5g of vitamin E, adding the weighed materials into the mixed solution obtained in the step 2), then adjusting the pH value to 5.5 by using 1% of sodium hydroxide solution, supplementing the remaining purified water until the total weight is 50g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
Example 3:
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 0.5g of carbomer 980 and 2.6g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 0.5g of the composite powder of the dendrobium officinale polysaccharide and the astragalus polysaccharide after ball milling, dissolving the composite powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 7.5g of diethylene glycol monoethyl ether, 50mg of methyl paraben, 0.5g of vitamin C and 0.5g of vitamin E, adding the weighed materials into the mixed solution obtained in the step 2), then adjusting the pH value to 6 by using 1% of sodium hydroxide, supplementing the rest purified water until the total weight is 50g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
As can be seen from comparative examples 1 to 3, when the pH of the gel reached 6, the gel was more uniform and fine, had a moderate viscosity, had a comfortable skin feel, and had good spreadability.
Example 4:
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 0.5g of carbomer 980 and 5.6g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 0.5g of the composite powder of the dendrobium officinale polysaccharide and the astragalus polysaccharide after ball milling, dissolving the composite powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 7.5g of diethylene glycol monoethyl ether, 75mg of methyl paraben, 0.5g of vitamin C and 0.5g of vitamin E, adding the weighed materials into the mixed solution obtained in the step 2), then adjusting the pH value to 6 by using 1% of sodium hydroxide, supplementing the remaining purified water until the total weight is 50g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
Example 5 (with low dose of complex polysaccharide):
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 1.0g of carbomer 980 and 5.6g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 1.0g of the composite powder of the dendrobium officinale polysaccharide and the astragalus polysaccharide after ball milling, dissolving the composite powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 10.0g of diethylene glycol monoethyl ether, 193.3mg of methyl paraben, 0.5g of vitamin C and 0.5g of vitamin E, adding into the mixed solution obtained in the step 2), then adjusting the pH to 6 by using 1% of sodium hydroxide, supplementing the remaining purified water until the total weight is 100g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
Example 6 (containing moderate doses of complex polysaccharide):
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 1.0g of carbomer 980 and 5.2g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 2.0g of the ball-milled composite powder of dendrobium officinale polysaccharide and astragalus polysaccharide, dissolving the powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 7.6g of diethylene glycol monoethyl ether, 193.3mg of methyl paraben, 1g of vitamin C and 1g of vitamin E, adding the weighed materials into the mixed solution obtained in the step 2), then adjusting the pH value to 6 by using 1% of sodium hydroxide, supplementing the rest purified water until the total weight is 100g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel.
Example 7 (containing high dose of complex polysaccharide):
a preparation method of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
1) weighing 1.0g of carbomer 980 and 5.7g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 4.0g of the composite powder of the dendrobium officinale polysaccharide and the astragalus polysaccharide after ball milling, dissolving the composite powder in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 10g of diethylene glycol monoethyl ether, 222.2mg of methyl paraben, 0.5g of vitamin C and 0.6g of vitamin E, adding the mixture into the mixed solution obtained in the step 2), then adjusting the pH to 6 by using 1% of sodium hydroxide, supplementing the remaining purified water until the total weight is 100g, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for later use.
As can be seen from comparative examples 5-7, when the content of Dendrobium officinale polysaccharide/Astragalus polysaccharide is increased, the gel has a brownish black appearance, and after standing for a period of time, a delamination phenomenon occurs. When the drug content is between 1% and 2%, the gel presents a good appearance shape and has good stability.
Example 8 (blank gel):
a preparation method of a blank gel matrix hydrogel comprises the following steps:
1) weighing 1.0g of carbomer 980 and 4.9g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring and swelling for 24h to obtain a blank gel matrix;
2) weighing 9.9g of diethylene glycol monoethyl ether, 200mg of methyl paraben, 0.6g of vitamin C and 0.6g of vitamin E, adding the weighed materials into the blank gel matrix obtained in the step 1), then adjusting the pH to 6 by using 1% of sodium hydroxide, supplementing the remaining purified water until the total weight is 100g, and uniformly mixing to obtain the blank gel matrix hydrogel for later use.
Example 9 (without astragalus polysaccharides):
a preparation method of dendrobium officinale polysaccharide hydrogel comprises the following steps:
1) weighing 0.5g of carbomer 980 and 2.5g of 1, 2-propylene glycol, adding part of purified water, and uniformly stirring for swelling for 24h to obtain a blank gel matrix;
2) weighing 2.0g of ball-milled dendrobium officinale polysaccharide, dissolving the dendrobium officinale polysaccharide in purified water, uniformly stirring, adding the mixture into the blank gel matrix obtained in the step 1), and uniformly stirring;
3) weighing 5.1g of diethylene glycol monoethyl ether, 109.3mg of methyl paraben, 0.25g of vitamin C and 0.25g of vitamin E, adding the weighed materials into the mixed solution obtained in the step 2), then adjusting the pH value to 6 by using 1% of sodium hydroxide, supplementing the remaining purified water until the total weight is 50g, and uniformly mixing to obtain the dendrobium officinale polysaccharide hydrogel for later use.
The preparation method of the ball-milled dendrobium officinale polysaccharide added in the step 2) comprises the following steps:
s1: drying the dendrobium officinale stems in an oven at 60 ℃ to constant weight, crushing, sieving by a third sieve, then placing in a planetary ball mill, wherein the ball milling speed is 300rpm, the ball milling time is 5min, and the filling rate of the planetary ball mill is 20.9 percent calculated by the ratio of the total volume of the added stainless steel balls to the volume of a ball milling tank; finally, obtaining the milled dendrobium officinale powder;
s2: weighing and placing the ball-milled dendrobium officinale powder in a round-bottom flask, adding extraction solvent water into the round-bottom flask, and extracting twice under the heating condition of 70 ℃, wherein the extraction time is 2 hours each time, the solid-liquid ratio during each extraction is 1:40, and the unit of the solid-liquid ratio is g/mL; and combining the two extracting solutions, performing rotary evaporation to obtain an extract, adding 95% ethanol according to a solid-to-liquid ratio of 1:40, wherein the solid-to-liquid ratio unit is g/mL, precipitating with ethanol overnight, performing centrifugal filtration, and drying to obtain the ball-milled dendrobium officinale polysaccharide.
Experiment one: the percutaneous permeation test of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel comprises the following steps:
the experimental steps are as follows:
(1) the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel obtained in the example 4 is placed in a refrigerator at 4 ℃ for storage.
(2) Soaking abdominal skin of rat with physiological saline, drying by filter paper, and stretching between two chambers (i.e. receiving chamber and blank chamber) with Valian-Chien double-chamber penetration, dermis layer facing the receiving chamber, and epidermis layer (area of 4 cm)2) 2g of gel (i.e., the Dendrobium officinale polysaccharide/Astragalus polysaccharide composite hydrogel of example 4) was applied with the epidermal layer facing the blank chamber. Fixing the two ports of the double chamber by a spring clip, adding 12mL of normal saline into the receiving chamber, performing ultrasonic treatment before use, circulating water bath temperature (32 +/-0.5) DEG C, placing 1 particle of stirring particles in the receiving chamber, and stirring at the rotating speed of 200 r/min. 2mL of the permeate was aspirated from the receiving chamber at 0.5, 1,2, 4, 6, and 8h, respectively, and 2mL of saline was added. Filtering the obtained solution to be tested with 0.22 μm water phase microporous membrane, and measuring polysaccharide concentration by phenol-sulfuric acid color development method.
(3) And (3) calculating the cumulative drug permeation amount Q in unit area according to a formula, drawing by taking Q as a vertical coordinate and t as a horizontal coordinate, and obtaining a cumulative drug permeation Q-t curve as shown in figure 1. According to the Q-t curve result in the figure 1, performing linear regression on the Q-t curve within the range of t being 2-6 h, wherein the slope of a straight line obtained by the linear regression is the drug obtaining permeation rate J, and the intersection point of the straight line obtained by the linear regression and the abscissa is the lag time ttag。
Wherein, the calculation formula of the medicine permeation quantity Q in unit area is as follows:
q: drug permeation per unit area (mg/cm)2)；CnThe polysaccharide concentration in the receiving solution measured for the nth sample(mg/mL)，CiThe polysaccharide concentration (mg/mL) in the receiving solution was determined for the ith sample; v: receiving fluid volume (mL); vi: sample volume (mL) at ith time; a: effective transmission area (cm)2)。
The experimental results are as follows: the transdermal drug permeation amount of the Dendrobium officinale polysaccharide/Astragalus polysaccharide composite gel is shown in figure 1, and the transdermal drug permeation rate is 0.69mg cm-2·h-1The lag time is 1.36 h. Indicating that the gel has good transdermal permeability.
And (2) test II: dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel auricle swelling test and pathological morphology influence:
the test steps are as follows:
(1) the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel obtained in example 5, example 6 and example 7 and the blank gel matrix hydrogel obtained in example 8 were stored in a refrigerator at 4 ℃.
(2) After 1W of adaptive feeding of 30 mice. 30 mice were randomly divided into 5 groups of 6 mice each, each group being: a blank control group, a positive control administration group, a high-dose compound polysaccharide administration group, a medium-dose compound polysaccharide administration group and a low-dose compound polysaccharide administration group. Among them, mice of the blank control group were administered with the blank gel matrix hydrogel (example 8), and mice of the positive control group were administered with the aloe gel. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel of example 7, example 6 and example 5 was administered to mice in the high-dose composite polysaccharide administration group, the medium-dose composite polysaccharide administration group and the low-dose composite polysaccharide administration group, respectively.
Smearing 0.2g of blank gel on the right ear of the mouse of the blank control group, smearing 0.2g of aloe gel on the right ear of the mouse of the positive control administration group, and smearing 0.2g of dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel on the right ear of the mouse of the other groups. On the 7 th day of the administration treatment, the right ear of the mouse is smeared with 50 mu L of xylene to cause inflammation for 40min, then the cervical vertebra is killed, the left ear is taken as the self control, the ear is cut off along the outline of the ear of the mouse, a 6mm puncher is used for punching the symmetrical part of the ears, the weight is calculated, and the weight of the average swelling degree of the right ear of the mouse is obtained by weighing.
Mouse auricle swelling inhibition rate calculation formula: the inhibition ratio of auricle swelling = (average swelling degree of blank group-average swelling degree of administration group)/average swelling degree of blank group × 100%.
The results of the experiment on the inhibition rate of drug on the swelling of auricle of mouse after the 5 groups of mice were administered with the drug are shown in table 1.
Table 1: inhibition rate of auricle swelling in mice
As can be seen from table 1: the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel has a good anti-inflammatory effect.
(3) And (3) putting the ear piece obtained by ear swelling of the mouse in the second test into a paraformaldehyde solution for fixing for later use. The fixed ear pieces were subjected to conventional paraffin sectioning, and HE staining (20X) was performed, observed with an optical microscope, and photographed.
The experimental results are as follows: the pathological form of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel is influenced as shown in fig. 2, the normal group is the experimental result of a normal mouse without any gel preparation, and the morphological structure of auricle epidermis, dermis and subcutaneous tissues of the mouse is normal, the tissue structure is compact, and no looseness or inflammatory cell infiltration is seen. The model group is the experimental result of the mice of the blank control group administered with the blank gel matrix hydrogel (example 8), and the pinna of the mice in the model group are obviously thickened, the subcutaneous tissues are loose, and inflammatory cell infiltration can be seen. The positive control group is the experimental result of the mice administered with the aloe gel. The high dose group, the medium dose group, and the low dose group are experimental results of mice administered with the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel of example 7, example 6, and example 5, respectively.
As can be seen from FIG. 2, the swelling of auricles and inflammatory cell infiltration were improved to various degrees in the mice of each administration group.
And (3) test III: the test method for the capacity of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel for eliminating DPPH free radicals comprises the following steps:
(1) the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel of the embodiment 6 and the dendrobium officinale polysaccharide hydrogel of the embodiment 9 are respectively stored in a refrigerator at 4 ℃.
(2) Diluting the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel obtained in the embodiment 6 with distilled water to prepare a series of gel aqueous solutions with the concentrations of the dendrobium officinale polysaccharide/astragalus polysaccharide composite polysaccharide being 0.5mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 3mg/mL and 4mg/mL respectively. Diluting the dendrobium officinale polysaccharide hydrogel in example 9 with distilled water to prepare a series of gel aqueous solutions with the dendrobium officinale polysaccharide concentrations of 0.5mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 3mg/mL and 4mg/mL respectively. Meanwhile, vitamin C is used as a positive control, and is diluted by distilled water to prepare a series of gel aqueous solutions with vitamin C concentrations of 0.5mg/mL, 1mg/mL, 1.5mg/mL, 2mg/mL, 3mg/mL and 4mg/mL respectively.
In performing the test for DPPH radical scavenging activity:
the preparation process of the sample solution comprises the following steps: accurately sucking 100. mu.L of the gel aqueous solution prepared above into a 96-well plate, and adding 100. mu.L of 0.2mmol/mL DPPH-ethanol solution.
The preparation process of the control solution is as follows: accurately pipette 100. mu.L of the aqueous gel solution into a 96-well plate, and add 100. mu.L of ethanol.
The preparation process of the blank solution comprises the following steps: accurately, 100. mu.L of distilled water was pipetted into a 96-well plate, and 100. mu.L of a 0.2mmol/mL solution of DPPH-ethanol was added.
After the prepared sample solution, the prepared control solution and the prepared blank solution are respectively shaken up, the light-shielding reaction is carried out for 20min, the absorbance A is measured at 517nm, three groups of parallel tests are simultaneously carried out, and the results are averaged. The clearance of the polysaccharide was calculated by the following formula. Vitamin C was used as a positive control.
DPPH radical scavenging Activity (%) ═ 1- (A)Sample (I)-AControl)*100％/ABlank space
In the above formula: a. theSample (I): absorbance of DPPH-ethanol solution and sample solution; a. theControl: absorbance of the sample solution and absolute ethanol; a. theBlank space: absorbance of DPPH-ethanol solution and distilled water.
And (3) test results: the activity of dendrobium officinale polysaccharide/astragalus polysaccharide on DPPH free radical scavenging is shown in figure 3, and a remarkable dose-dependent relationship is presented. When the dendrobium officinale polysaccharide and the astragalus polysaccharide are used together, the DPPH free radical scavenging activity is better than that of the dendrobium officinale polysaccharide gel which is used alone.
In conclusion, the dendrobium officinale polysaccharide and the astragalus polysaccharide are extracted in an auxiliary manner by a ball milling method, the obtained dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel has the effects of moisturizing, oxidation resistance and inflammation resistance, and pathological detection also proves that the gel obviously improves auricle swelling and inflammatory cell infiltration and improves acute inflammation. Because the active component is the plant extract component, the prepared gel has no irritation to skin and mild property.
The statements in this specification merely set forth a list of implementations of the inventive concept and the scope of the present invention should not be construed as limited to the particular forms set forth in the examples.
Claims (9)
1. A Dendrobium officinale polysaccharide/Astragalus polysaccharide composite hydrogel is characterized in that the prescription of the preparation takes the composite polysaccharide of Dendrobium officinale polysaccharide and Astragalus polysaccharide as the main medicine; the preparation comprises the following components in percentage by mass: 1 to 2 percent of main medicine, 0.5 to 1.8 percent of gel matrix, 5 to 15 percent of skin penetration enhancer, 5 to 10 percent of humectant, 1 to 2 percent of antioxidant, 0.1 to 0.2 percent of preservative and the balance of water, and the pH value is adjusted to be 6 by a pH regulator.
2. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the gel matrix is at least one selected from acacia, gelatin, carbomer 980, sodium alginate, CMC-Na and HPMC, preferably carbomer 980.
3. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the humectant is at least one selected from 1, 2-propylene glycol and glycerin, preferably 1, 2-propylene glycol.
4. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the skin penetration enhancer is selected from diethylene glycol monoethyl ether.
5. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the preservative is at least one selected from methyl paraben, ethyl paraben and sodium benzoate, preferably methyl paraben.
6. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the antioxidant comprises vitamin C and vitamin E, and the mass ratio of the vitamin C to the vitamin E is 1: 1.
7. The dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, wherein the pH adjusting agent is selected from sodium hydroxide or citric acid, preferably sodium hydroxide.
8. The preparation method of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel as claimed in claim 1, which is characterized by comprising the following steps according to a composition formula:
1) drying the dendrobium officinale stems and the astragalus roots to constant weight, crushing, sieving, then placing the mixture into a planetary ball mill according to the mass ratio of 1:1, wherein the ball milling speed is 300rpm, the ball milling time is 5min, and the filling rate of the planetary ball mill is 20.9 percent calculated by the ratio of the total volume of the added stainless steel ball milling beads to the volume of a ball milling tank; finally, obtaining the milled dendrobium officinale/astragalus powder;
2) weighing and placing the ball-milled dendrobium officinale/astragalus membranaceus powder into a round-bottom flask, adding an extraction solvent water into the round-bottom flask, and extracting twice under the heating condition of 70 ℃, wherein the extraction time is 2 hours each time, the solid-liquid ratio in each extraction is 1:40, and the unit of the solid-liquid ratio is g/mL; mixing the two extracting solutions, carrying out rotary evaporation to obtain an extract, adding 95% ethanol according to a solid-to-liquid ratio of 1:40, wherein the solid-to-liquid ratio unit is g/mL, carrying out alcohol precipitation overnight, carrying out centrifugal filtration, and drying to obtain the compound polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide;
3) weighing a gel matrix, dissolving the gel matrix in part of purified water, adding a humectant, uniformly stirring, and swelling for 24 hours to obtain blank gel;
4) weighing the compound polysaccharide of the dendrobium officinale polysaccharide and the astragalus polysaccharide obtained in the step 2), dissolving the compound polysaccharide in part of purified water, adding the blank gel obtained in the step 3), and uniformly stirring;
5) weighing an antioxidant, a skin-promoting penetrating agent and a preservative, adding into the mixed solution obtained in the step 4), adjusting the pH to 6 with a pH regulator, supplementing the rest purified water, and uniformly mixing to obtain the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel.
9. The use of the dendrobium officinale polysaccharide/astragalus polysaccharide composite hydrogel of claim 1 in the preparation of a pharmaceutical preparation or a cosmetic preparation for reducing skin inflammation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113952251A (en) * | 2021-09-26 | 2022-01-21 | 无限极（中国）有限公司 | Polysaccharide composition with function of regulating skin microecology and preparation method and application thereof |
| CN114306610A (en) * | 2022-02-08 | 2022-04-12 | 安徽世龙生物医药科技有限公司 | External gel preparation for quickly removing diseased nails and preparation method thereof |
| CN117143264A (en) * | 2023-10-30 | 2023-12-01 | 中国医学科学院药用植物研究所 | Acetylated astragalus polysaccharide, preparation method and application thereof in preparation of hydrogel for promoting wound healing |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160058723A1 (en) * | 2013-04-04 | 2016-03-03 | Hyundai Pharm Co., Ltd. | Composition for external use preparation with improved transdermal permeability |
| US20170274008A1 (en) * | 2016-03-24 | 2017-09-28 | Hong Kong Baptist University | Use of polysaccharide in Dendrobium officinale for anti-fatigue |
| CN108464960A (en) * | 2018-04-17 | 2018-08-31 | 凯瑞欧（天津）生物技术有限公司 | A kind of dendrobium candidum beauty-care gel |
-
2021
- 2021-03-30 CN CN202110340217.7A patent/CN113018202A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160058723A1 (en) * | 2013-04-04 | 2016-03-03 | Hyundai Pharm Co., Ltd. | Composition for external use preparation with improved transdermal permeability |
| US20170274008A1 (en) * | 2016-03-24 | 2017-09-28 | Hong Kong Baptist University | Use of polysaccharide in Dendrobium officinale for anti-fatigue |
| CN108464960A (en) * | 2018-04-17 | 2018-08-31 | 凯瑞欧（天津）生物技术有限公司 | A kind of dendrobium candidum beauty-care gel |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113952251A (en) * | 2021-09-26 | 2022-01-21 | 无限极（中国）有限公司 | Polysaccharide composition with function of regulating skin microecology and preparation method and application thereof |
| CN113952251B (en) * | 2021-09-26 | 2023-09-05 | 无限极（中国）有限公司 | Polysaccharide composition with skin microecology regulating effect and preparation method and application thereof |
| CN114306610A (en) * | 2022-02-08 | 2022-04-12 | 安徽世龙生物医药科技有限公司 | External gel preparation for quickly removing diseased nails and preparation method thereof |
| CN117143264A (en) * | 2023-10-30 | 2023-12-01 | 中国医学科学院药用植物研究所 | Acetylated astragalus polysaccharide, preparation method and application thereof in preparation of hydrogel for promoting wound healing |
| CN117143264B (en) * | 2023-10-30 | 2024-02-20 | 中国医学科学院药用植物研究所 | Acetylated astragalus polysaccharide, preparation method and application thereof in preparation of hydrogel for promoting wound healing |
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