WO2012089101A1

WO2012089101A1 - Anti-aging traditional chinese medicinal herbal composition and preparation method and use thereof

Info

Publication number: WO2012089101A1
Application number: PCT/CN2011/084734
Authority: WO
Inventors: 祝乐; 熊卫国; 陈默; 程康; 赵亚; 严淑贤; 任婕; 项红蕾; 方程; 吕洛; 魏少敏
Original assignee: 上海家化联合股份有限公司
Priority date: 2010-12-31
Filing date: 2011-12-27
Publication date: 2012-07-05
Also published as: CA2823422C; CN102552420A; AU2011352576B2; CN102552420B; CA2823422A1

Abstract

Disclosed is a traditional Chinese medicinal herbal extract comprising extracts in water or solvent of 10 to 50 parts by weight of Saussurea involucrate, 10 to 50 parts by weight of Cistanche deserticola and 20 to 60 parts by weight of Astragalus membranaceus. The solvent is selected from methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof. The extract and the traditional Chinese medicinal herbal composition containing the extract have the effects of anti-oxidation, promoting dermal fibroblast proliferation and anti-UV irradiation, and can be used as active components in the preparation of cosmetics for preventing skin aging, particularly photo-aging.

Description

Anti-aging Chinese herbal medicine composition and preparation method and application thereof

The invention relates to the field of cosmetics, in particular to a Chinese herbal medicine extract, a preparation thereof and an application thereof, and a Chinese herbal medicine composition having anti-aging, especially anti-photoaging effect, comprising the Chinese herbal medicine extract, and preparation thereof and application in cosmetics. Background technique

Studies have shown that skin photoaging is mainly caused by UVB (UVB, 290~320nm) and long-wavelength UV (UVA, 320~400nm) in sunlight. Ultraviolet light has a significant effect on the aging of human skin. The risk of skin aging in high-exposure populations is twice as high as that in low-exposure populations, and the aging time is 10 years earlier. Long-term exposure to sunlight and ultraviolet light causes skin photoaging, which can lead to serious cosmetic problems. In recent years, it has received much attention from the industry. Products resistant to photoaging have come out, and related research such as fire is like tea. With the requirements of natural, safety and environmental protection, green cosmetics are increasingly favored by people. However, at present, anti-photoaging cosmetics in domestic and foreign markets mostly use biochemical preparations and synthetic compounds as main functional support additives. The anti-photoaging additives of Chinese herbal medicines with certain Chinese medicine theory are still vacant. Summary of the invention

The technical problem to be solved by the present invention is to provide a Chinese herbal medicine extract.

The Chinese herbal medicine extract of the present invention is an aqueous or solvent extract of 10 to 50 parts by weight of snow lotus, 10 to 50 parts by weight of Cistanche, and 20 to 60 parts by weight of Astragalus, wherein the solvent is selected from the group consisting of methanol, ethanol, and isopropanol. , n-butanol, ethylene glycol, 1,2-propanediol, 1, 3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof.

Among the above formulas, snow lotus, Cistanche, and Astragalus have clear anti-aging effects.

In the present invention, snow lotus refers to the medicinal part of the flower of the plant snow lotus. Snow lotus is a herbaceous plant of the genus Chrysanthemum of the genus Chrysanthemum. It mainly includes Saussurea involucrata Kar. et Kir., Saussurea laniceps Hand. -Mazz., S. gnaphaloides (Royle) ) Sch.-Βίρ., Jellyfish Snow Lotus S. medusa ro'. Snow Lotus is mainly produced in Sichuan, Yunnan, Tibet, Xinjiang, Gansu, Qinghai, etc., from June to ^July , when the flowers are open, Plant, remove the soil, dry, cut, and use.

Snow Lotus heats and nourishes blood, balances yin and yang, and replenishes the benefits. More than a thousand years ago, "Tao Zang" called Snow Lotus "Millennium Fairy Grass", "Reminiscence of the Old Poetry": "Snow Lotus, the Millennium is not deep in the snow. It is shaped like a lotus flower, and it can be made by high-quality wine. It is made by brewing and multiplying spring color. Covering the cathode and the meaning of Yangsheng. "The Compendium of the Compendium": "Snow Lotus, the north of the Ili and Jinchuan, the place of the cold, Ji Xuechun Summer is not scattered, there are grass in the snow, lotus-like, single stem, lovely in the pavilion."

Cistanche is a medicinal part of the dried scaly fleshy stem of Cistanche tubulosa or Cistanche tubulosa. Cistanche is a dry, scaly fleshy stem of the Cistanche deserticola Y. C. Ma or Cistanche tubulosa (Schrenk) Wight. More than the spring seedlings were not excavated or excavated when they were just released, the inflorescences were removed, cut into sections, and dried. Cistanche is mainly distributed in Inner Mongolia, Gansu, Xinjiang, Qinghai and other places.

Cistanche is contained in "Shen Nong's Herbal Classic". According to the description: Cistanche is sweet, salty, and warm. It has the functions of tonifying kidney yang, benefiting essence blood, and relaxing bowel movements. It treats impotence, infertility, blood stasis, and weak waist and knees. Cold pain, weakness and other symptoms. In the past dynasties, Cicada was used as a royal treasure for the tribute to the courts of the Western Regions. Cistanche is a kidney that nourishes blood and regulates color, can regulate endocrine, promote metabolism and strengthen the body, and has anti-oxidation effect. "Pharmacology": "Yi marrow, Yue color, Yannian, Dabuyi" "Complete the defeat, face black labor injury."

Astragalus, also known as Astragalus, is a collective name for plants and Chinese herbal medicines. Astragalus membranaceus refers to the medicinal part of the dry root of the plant Mongolian Astragalus or Astragalus membranaceus. Astragalus is a leguminous plant Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. j thousand), ^ root. In the spring and autumn seasons, the roots and roots are removed and dried. Astragalus membranaceus is produced in North China, Northeast China, Inner Mongolia and Northwest China. It is mainly produced in Shanxi, Heilongjiang, Liaoning, Hebei and other provinces. Sichuan, the former Soviet Union, North Korea and Mongolia are also distributed. The membranous scutellariae is mainly distributed in the northeastern, northern China, Gansu, Sichuan, Tibet and other provinces; Mongolian sorghum is mainly produced in Inner Mongolia, Shanxi and Heilongjiang; it is widely cultivated.

The Chinese medicinal material Astragalus membranaceus has the effects of tonifying the qi, solidifying the water, reducing the swelling of the water, removing the pus, and stimulating the muscles. The medicinal use of Astragalus has been more than 2,000 years old. Modern research, Astragalus saponins, sucrose, polysaccharides, various amino acids, folic acid and Shixi, Ci, copper and other trace elements. It has enhanced immune function, liver protection, diuresis, anti-aging, anti-stress, antihypertensive and broader antibacterial effects. Astragalus replenishing in Yanze noodles can enhance the body's resistance, anti-oxidation and delay aging.

This side of the jaundice balances nourishing qi and blood, enhances skin immunity as a medicinal herb; Snow lotus nourishing blood, fighting against light radiation and oxidation, used as a drug; combined with ancient beauty literature research, increasing or decreasing compatibility, choosing the reasonable ratio of Astragalus, Cistanche, and Snow Lotus, using the roots, stems, and flowers of medicinal plants Resist skin aging. The effect is comprehensive, and its composition is more suitable for the synergistic effect of traditional Chinese medicine in cosmetics than single Chinese medicine.

Preferably, the Chinese herbal medicine extract of the present invention is 30 parts by weight of snow lotus, 20 parts by weight of Cistanche, 50 parts by weight of water or a solvent extract of xanthine, and the solvent is selected from the group consisting of methanol, ethanol, isopropanol, n-butanol, Ethylene glycol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof.

Preferably, the extraction is selected from the group consisting of boiling, refluxing, dipping, percolating, or a combination thereof.

The above extract may be in a form selected from the group consisting of a solution, a powder, an ointment, a film or a cream. The present invention also provides a Chinese herbal medicine composition having an anti-photoaging effect, the composition comprising the above extract.

The Chinese herbal medicine extract and the Chinese herbal medicine composition of the present invention can be directly used for preventing skin aging, especially photoaging, and can also be added to cosmetics as an efficacy additive. The Chinese herbal medicine extract and the Chinese herbal medicine composition of the invention have anti-aging effect, especially anti-photoaging effect, help the skin to resist oxidation, promote the proliferation of dermal fibroblasts, especially help the skin to resist UVB and UVA ultraviolet radiation, protect the skin, reduce damage, It prevents skin photoaging and is non-irritating to the skin. The cosmetic may be a cosmetic cream, an eye cream, a facial mask, a facial cleanser, a toner or a serum. The invention also provides a preparation method of the above Chinese herbal medicine extract, the method comprising:

10 to 50 parts by weight of snow lotus, 10 to 50 parts by weight of Cistanche deserticola and 20 to 60 parts by weight of astragalus, thoroughly mixed with water and/or solvent for 0.5 to 1 hour and extracted, the solvent is selected from the group consisting of decyl alcohol, ethanol, and isopropyl alcohol. Alcohol, n-butanol, ethylene glycol, 1,2-propanediol, 1, 3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof;

Filtering, and repeatedly extracting the filter residue, and combining the filtrates;

Concentrate the filtrate to about twice the total weight of the medicinal material (V/W). The doubling amount refers to the ratio of the volume of the solvent to the total weight of the medicinal material, wherein the unit of the solvent volume is ml, and the unit of the total weight of the medicinal material is g; Shen

The alcohol precipitation supernatant recovers the solvent.

The alcohol precipitate can be formulated into various dosage forms such as solutions, powders, ointments, films or creams. Preferably, the alcohol precipitation is selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1 > 2- An alcohol of propylene glycol, 1,3-propanediol, 1, 3-butanediol or a combination thereof.

The extract of the present invention can be added with water to prepare a composition containing a crude drug amount of 50 to 1000 mg/ml. detailed description

The invention will now be described in greater detail by way of examples.

Example 1

Take pure Chinese herbal medicine snow lotus 10g, astragalus 60g, Cistanche 30g total 100g, mix well, add 10 times the amount (multiple: refers to the ratio of solvent volume to the total weight of the drug, where the unit volume of the solvent is ml, the unit of total weight of the drug It is g, the same as below) 75% ethanol (v/v) is cold-immersed for 48 hours, filtered, and the filtrate is recovered into ethanol, and the obtained extract is dissolved in water to prepare a solution containing a crude drug amount of 500 mg/ml to obtain an extract A. Example 2

Take pure Chinese herbal medicine snow lotus 30g, astragalus 30g, Cistanche 40g total 100g, mix well, add 10 times methanol to reflux extraction for 2 hours, filter, the filtrate recovers sterol, the obtained extract is dissolved in water, and the crude drug content is 50mg. /ml of the solution to obtain extract B. Example 3

Take pure Chinese herbal medicine snow lotus 40g, astragalus 50g, Cistanche 10g total 100g, mix well, decoction with 10 times the amount of water for about 2 hours, concentrate, filter, the supernatant is added with water to make a crude drug content of 1000mg / ml Solution, to obtain extract C. Example 4

Take pure Chinese herbal medicine snow lotus 30g, astragalus 40g, Cistanche 30g total 100g, mix well, decoction and extract with 10 times of water for about 2 hours, concentrate, add 2 times the amount of ethanol precipitation, filter, the filtrate recovers ethanol, the obtained extract The solution was dissolved in water to prepare a solution containing a crude drug amount of 500 mg/ml to obtain an extract 0. Experimental Example 1 DPPH experiment

Free radicals are a class of chemical substances that are active and highly oxidizing. Ultraviolet light UVA/UVB irradiation produces a large amount of free radicals, causing damage to the body and accelerating aging. Here, the in vitro antioxidant activity of the extracts in the examples was determined by DPPH radical scavenging method. The extracts in the examples were diluted to two concentrations of 1% and 0.5% (v/v), respectively, with distilled water. Then, 2 ml of the extract of the example, a concentration of 50 (^mol/L of DPPH ethanol solution 0.5 ml and 1.5 ml of water were added to the same test tube, shaken, and allowed to stand. After 30 min, the absorbance (OD) was measured at 517 nm. And the corresponding mixture was used as a blank control. The clearance rate of DPPH of the example extract was calculated according to the following formula:

T - TO

Clearance rate (%) 100 %

C where:

The absorbance of the extract of the T-DPPH+ example,

TO—Example extract + solvent absorbance,

The absorbance of C-DPPH+ solvent. The antioxidant capacity evaluation of the anti-aging Chinese herbal medicine composition of the present invention is shown in Table 1 ₍

Table 1

The experimental results showed that the extract A of Example 1, the extract B of Example 2, the extract C of Example 3, and the extract D of Example 4 were 1 °/ in vitro. And 0.5% concentration have better anti-oxidation ability to scavenge free radicals, wherein 0.5% of free radical scavenging rate is above 15%, and 1% concentration of free radical scavenging rate is above 35%, with the extract of Example 4 D works best. Experimental Example 2 Fibroblast proliferation test The fibroblasts in the skin are the main cells for the synthesis of collagen. The proliferation of fibroblasts or the enhancement of the ability of fibroblasts to synthesize and secrete collagen can increase the total amount of collagen, thereby slowing down the aging of the skin. Therefore, research on the proliferative effect of fibroblasts is one of the important methods for evaluating the anti-aging activity of samples. According to the above mechanism of skin aging, the method of in vitro culture of human fibroblasts is used to initially evaluate the anti-aging effect of the traditional Chinese medicine composition of the present invention.

The prepared cell suspension (prepared to a concentration of 5*10 ³ /ml) was inoculated into a 96-well plate, and after the cells were attached, 20 ul of the corresponding concentration of the Chinese herbal medicine of the example was added to each well except the blank control group. Extract, set 4 concentration groups, 6 holes in each group, incubate in incubator (37 ° C, 5% C0 ₂ ) for 24 h, then add 20 ul of MTT solution, continue in the incubator (37 ° C, 5 Incubate for 4 h in %C0 ₂ ). After removing the microplate, carefully remove the culture solution from each well, add DMSO, and mix well. The optical density (570 nm) was measured by enzyme-linked immunosorbent assay, and the relative proliferation rate of the sample group was obtained by comparison with the control group.

The results of the proliferative activity of the anti-aging Chinese herbal composition against fibroblasts are shown in Table 2.

Table 2

The experimental results show that the extract A of Example 1, the extract B of Example 2, the extract C of Example 3, and the extract D of Example 4 all promote the proliferation of fibroblasts, and the effect of extract C of Example 3 is the most Well, its 0.001% concentration contributes to the proliferation of fibroblasts by 10.4%. Experimental Example 3 Protective effect of UV irradiation on fibroblast injury

Skin photoaging is mainly caused by medium-wave ultraviolet (UVB, 290~320nm) and long-wave ultraviolet (UVA, 320~400nm) in sunlight, which has a significant effect on the aging of human skin. UVA/UVB irradiation causes damage to fibroblasts, causing damage to the skin and causing photoaging. Here, the UV-irradiation experiment was used to examine the photoaging effect of the herbal extract of the examples against UV-induced cell protection.

Take 0.5ml of the extract in the example, dilute with 4.5ml of PBS, and mix it with 1/10 concentration (v/v) for suction filtration, then take 0.1ml force p 9.9ml sterile PBS and finally dilute to 1/1000. concentration (v/v) „ Skin fibroblasts (Fb) cultured in normal DMEM were used as a control group, and UVA (5-20 J/cm ² )/UVB UVB (20-80 J/cm ² ) was cultured in normal DMEM. The irradiated Fb cells were used as a model group, and the Fb cells co-cultured with the sample extract (0.15 mg/ml) and DMEM were irradiated with UVA/UVB as the drug-added group. 24 hours after the ultraviolet treatment, the enzyme was labeled with CCK-8. The absorbance (OD value) of each well was measured, and the cell proliferation rate was the percentage of the OD value of each treatment group to the negative control group, and each test face was repeated three times.

The experimental results are shown in Tables 3 and 4. Table 3 UVA irradiation

The experimental results show that the Extract A of Example 1, the extract of Example 2, and the extract of Example 3 (:, Extract D of Example 4 can all reduce the damage of fibroblasts by UV irradiation, so that the cell proliferation rate is basically the same. The recovery was more than 80%, and the effect of Extract D of Example 4 was the best.

Claims

Claim

A Chinese herbal medicine extract characterized by comprising 10 to 50 parts by weight of snow lotus, 10 to 50 parts by weight of Cistanche, and 20 to 60 parts by weight of water or a solvent extract of xanthine, the solvent being selected from the group consisting of sterol and ethanol , isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1,3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof.

2. The herbal extract according to claim 1, wherein the extraction is selected from the group consisting of soaking, boiling, refluxing, percolating, or a combination thereof.

The herbal extract according to claim 1 or 2, wherein the herbal extract form is selected from the group consisting of a solution, a powder, an ointment, a film or a cream.

4. A Chinese herbal medicine composition having anti-aging effect, characterized by comprising the claims

The herbal extract of any of 1-1-3.

5. Use of a Chinese herbal composition according to claim 4 in cosmetics.

6. A method for preparing a Chinese herbal medicine extract, comprising the steps of:

10 to 50 parts by weight of snow lotus, 10 to 50 parts by weight of Cistanche and 20-60 parts by weight of Astragalus membranaceus, which is selected from the group consisting of water, methanol, ethanol, isopropanol, n-butanol, ethylene glycol, 1 ,

2-propanediol, 1,3-propanediol, 1,3-butanediol, acetone, ethyl acetate or a combination thereof.

7. The method according to claim 6, further comprising:

The filtrate is concentrated to about twice the total amount of the medicinal material (V/W), and the multiple is the ratio of the volume of the solvent to the total weight of the medicinal material, wherein the unit of the solvent volume is ml, and the unit of the total weight of the medicinal material is g;

Then carry out alcohol precipitation;

The alcohol precipitation supernatant recovers the solvent.

The method according to claim 6 or 7, wherein the alcohol precipitation is selected from the group consisting of decyl alcohol, ethanol, isopropanol, n-butanol, ethylene glycol, 1,2-propanediol, 1, An alcohol of 3-propanediol, 1, 3-butanediol or a combination thereof.

9. A herbal extract prepared by the method of any of claims 6-8.

The Chinese herbal medicine extract according to claim 9, wherein the Chinese herbal medicine extract form is selected from the group consisting of a solution, a powder, an ointment, a film or a cream.

11. A Chinese herbal medicine composition having anti-aging effects, characterized by comprising the claims The herbal extract of 9 or 10 is used.

12. Use of a Chinese herbal medicine extract according to claim 11 in cosmetics.

The use according to claim 5 or 12, wherein the cosmetic is a cosmetic skin cream, an eye cream, a facial mask, a facial cleanser, a toner or a serum.