CN103191172A - Gynostemma pentaphylla extract, and preparation method and application thereof - Google Patents
Gynostemma pentaphylla extract, and preparation method and application thereof Download PDFInfo
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- CN103191172A CN103191172A CN2012100025781A CN201210002578A CN103191172A CN 103191172 A CN103191172 A CN 103191172A CN 2012100025781 A CN2012100025781 A CN 2012100025781A CN 201210002578 A CN201210002578 A CN 201210002578A CN 103191172 A CN103191172 A CN 103191172A
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Abstract
The invention discloses a preparation method of a Gynostemma pentaphylla extract product. The method comprises the following steps: 1, extracting a raw material with a solvent, wherein the solvent is selected from water, methanol, ethanol, isopropanol, 1-butanol, glycol, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol, acetone, ethyl acetate or a combination thereof; 2, separating the obtained extract liquid and residues to obtain the extract liquid; 3, optionally recovering the solvent; and 4, optionally dissolving or diluting. The invention also discloses the Gynostemma pentaphylla extract obtained through using the method. The invention further discloses an application of the Gynostemma pentaphylla extract in the preparation of skin light ageing resistance cosmetics or medicines.
Description
Technical field
The present invention relates to Natural Medicine Chemistry field and cosmetic field, be specifically related to a kind of Chinese herbal medicine Herb Gynostemmae Pentaphylli for anti-light aging in cosmetics or the medicine.
Background technology
Studies show that free radical and ultraviolet all are one of factors of skin aging, and photoaging mainly by the ultraviolet B radiation in the daylight (UVB, 290～320nm) and long wave ultraviolet (UVA, 320～400nm) cause jointly.Ultraviolet has obvious facilitation to application on human skin aging, and high exposed population group's skin aging is dangerous to double than low exposed population group, and the time of origin that wears out shifts to an earlier date 10 years.Long term exposure causes skin photoage in the daylight ultraviolet, and can cause serious cosmetic problem, thereby extremely industry concern in recent years, the product of aging resistance and anti-light aging comes out one after another, correlational study is like a raging fire, but natural Chinese medicinal herb anti-light aging additive is still vacancy.
Summary of the invention
The object of the invention is to provide a kind of preparation method of Herb Gynostemmae Pentaphylli extract, and described method comprises:
(1) solvent extraction raw material, described solvent are selected from water, methanol, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain extracting solution;
(3) optional solvent recovery;
(4) optionally dissolving or dilution.
Preferably, wherein the extraction of step (1) is selected from immersion, decoction, backflow, percolation or its combination.
Preferably, wherein used solvent is selected from water, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1 in the step (4), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
Another purpose of the present invention is to provide the preparation method of another kind of Herb Gynostemmae Pentaphylli extract, and described method comprises:
(1) solvent extraction raw material, described solvent are selected from methanol, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain residue;
(3) residue of water extraction step (2).
Preferably, wherein the extraction of step (1) and (3) is independently selected from immersion, decoction, backflow, percolation or its combination.
Preferably, described preparation method also comprises:
(4) separate aqueous extract and residue, obtain aqueous extract;
(5) concentrate, the ethanol precipitate with ethanol separates obtaining precipitation;
(6) alternatively, the resolution of precipitate with step (5) is mixed with solution.
Preferably, wherein used solvent is selected from ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1 in the step (6), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
Another purpose of the present invention provides the Herb Gynostemmae Pentaphylli extract according to the preparation method acquisition of the invention described above.
Preferably, the form of described Herb Gynostemmae Pentaphylli extract is selected from solution, powder, ointment, membrane or cream frost.
The Herb Gynostemmae Pentaphylli extract that the method according to this invention obtains can be directly used in the prevention skin photoage, also can be used as functional additive or active component and joins in cosmetics or the medicine and use.
Further object of the present invention provides the Herb Gynostemmae Pentaphylli extract of prepared in accordance with the method for the present invention in the cosmetics of preparation anti-light aging or the purposes in the medicine.
Further object of the present invention provides a kind of cosmetics or cosmetics of anti-light aging, wherein contains the Herb Gynostemmae Pentaphylli extract of prepared in accordance with the method for the present invention.
In the present invention, Herb Gynostemmae Pentaphylli Gynostemma pentaphyllum (Thunb) Mak. refers to this medicinal part of herb of plant Herb Gynostemmae Pentaphylli.Herb Gynostemmae Pentaphylli is Cucurbitaceae Gynostemma herbaceos perennial, is born in the dark and damp place of intermountain, the distribution south China.Bitter in the mouth is cold in nature, returns spleen, lung meridian, and eliminating inflammation and expelling toxin, benefiting QI for activating blood circulation, pharmacological research show to have anti-aging effects, and has the effect of resisting fatigue, anti-hypoxia, high temperature resistance.
The Herb Gynostemmae Pentaphylli extract that method of the present invention makes can help skin to resist the ultraviolet radiation of UVB and UVA; protection skin; weaken the UV irradiation to the influence of dermal fibroblast propagation, collagen protein; reduce cell injury; prevent the minimizing of dermal collagen albumen; effectively prevent skin photoage, and to the skin nonirritant.
The specific embodiment
Embodiment 1
Herb Gynostemmae Pentaphylli Gynostemma pentaphyllum (Thunb) Mak.100g, adding 10 times of amounts (doubly measures: the ratio that refers to solvent volume and Herb Gynostemmae Pentaphylli weight, wherein the unit of solvent volume is ml, the unit of Herb Gynostemmae Pentaphylli weight is g, down with) 70% ethanol (v/v) reflux, extract, 1.5 hours, filter, filtrate recycling ethanol, the extract obtained water dissolution of using is made and is contained the solution that the crude drug amount is 1000mg/ml, obtains extracting solution A.
Embodiment 2
Herb Gynostemmae Pentaphylli Gynostemma pentaphyllum (Thunb) Mak.100g adds 10 times of amount 80% methanol, and reflux, extract, 1.5 hours filters, and filtrate is reclaimed methanol, and the extract obtained water dissolution of using is made and contained the solution that the crude drug amount is 500mg/ml, obtains extracting solution B.
Embodiment 3
Herb Gynostemmae Pentaphylli Gynostemma pentaphyllum (Thunb) Mak.100g decocts with 10 times of water gagings and to extract about 2 hours, concentrates, and filters, and supernatant adds water to be made and contain the solution that the crude drug amount is 1000mg/ml, obtains extracting solution C.
Embodiment 4
Herb Gynostemmae Pentaphylli Gynostemma pentaphyllum (Thunb) Mak.100g, adding 10 times of amounts (doubly measures: the ratio that refers to solvent volume and Herb Gynostemmae Pentaphylli weight, wherein the unit of solvent volume is ml, the unit of Herb Gynostemmae Pentaphylli weight is g, down together) 95% ethanol (v/v) reflux, extract, is 1 hour, filter, get filtering residue and add 10 times of deionized water heating extraction 1.5 hours, filter, get filtrate, concentrate, add 4 times of 95% ethanol precipitate with ethanol, filter and obtain precipitation, gained precipitation water dissolution, make and contain the solution that the crude drug amount is 1000mg/ml, obtain extracting solution D.
Embodiment 5UV irradiation is to the protective effect of fibroblast damage
Skin photoage mainly by the ultraviolet B radiation in the daylight (UVB, 290～320nm) and long wave ultraviolet (UVA, 320～400nm) cause jointly, ultraviolet has obvious facilitation to application on human skin aging.The UVA/UVB irradiation can cause that fibroblastic damage is dead, and skin is damaged, and causes photoaging.Investigate the Chinese herbal medicine extract of embodiment to the photoaging effect of resisting of the cytoprotective of UV irradiation at this by UV irradiation experiment.
Get extract 0.5ml among the embodiment respectively, add 4.5ml PBS and dilute, be made into 1/10 concentration (v/v) and carry out sucking filtration, get 0.1ml then and add 9.9ml sterile phosphate buffer (PBS) and be diluted to 1/1000 concentration (v/v) at last.Be matched group with normal DMEM liquid cultured skin fibroblast (Fb), with normal DMEM liquid culture fluid (Dulbecco ' s modified eagle medium) with UVA (5-20J/cm
2)/UVB UVB (20-80J/cm
2) the Fb cell of irradiation is model group, with the embodiment extract that contains 1/100 concentration with DMEM liquid is cultivated altogether and be the dosing group through the Fb cell of UVA/UVB irradiation.24h after the UV treatment measures the absorbance (OD value) in each hole by CCK-8 with microplate reader, and cell proliferation rate accounts for the percentage rate of negative control group OD value for each processed group OD value, and each tests triplicate.
Experimental result sees Table 1 and table 2.(the P value represents significant difference)
Table 1UVA irradiation
| Cell proliferation rate | The P value | |
| Embodiment 1 extract A | (91.72±6.09)％) | ＜0.01 |
| Embodiment 2 extract B | (89.13±5.38)％ | ＜0.01 |
| Embodiment 3 extract C | (89.25±3.20)％ | ＜0.01 |
| Embodiment 4 extract D | (89.80±3.46)％ | ＜0.01 |
| Matched group | (100±4.32)％ | |
| Model group | (74.24±3.78)％ | ＜0.01 |
Table 2UVB irradiation
| Cell proliferation rate | The P value | |
| Embodiment 1 extract A | (88.19±4.32)％ | ＜0.01 |
| Embodiment 2 extract B | (87.88±4.22)％ | ＜0.01 |
| Embodiment 3 extract C | (88.01±2.58)％ | ＜0.01 |
| Embodiment 4 extract D | (88.06±3.55)％ | ＜0.01 |
| Matched group | (100±5.68)％ | |
| Model group | (83.63±5.15)％ | ＜0.01 |
Experimental result shows, embodiment 1 extract A, embodiment 2 extract B, embodiment 3 extract C, embodiment 4 extract D all can reduce the UV irradiation to fibroblastic damage, the cell appreciation rate is all returned to more than 85%, and best with the effect of embodiment 1 extract A.
Skin photoage mainly by the ultraviolet B radiation in the daylight (UVB, 290～320nm) and long wave ultraviolet (UVA, 320～400nm) cause jointly, ultraviolet has obvious facilitation to application on human skin aging.The UVA/UVB irradiation can cause that fibroblastic damage is dead, and the minimizing of dermal collagen albumen, and skin is damaged.The present invention is with the UVA/UVB irradiation cultured skin fibroblast of certain intensity; hatch with Herb Gynostemmae Pentaphylli extract; can effectively reduce the UV irradiation to the influence of fibroblast proliferation; can effectively reduce simultaneously the variation of dermal fibroblast collagen protein; protection skin; illustrate that Herb Gynostemmae Pentaphylli extract has the protective effect that ultra-vioket radiation damages to skin flbroblast, can defend photoaging.
Claims (11)
1. the preparation method of a Herb Gynostemmae Pentaphylli extract comprises:
(1) solvent extraction raw material, described solvent are selected from water, methanol, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain extracting solution;
(3) optional solvent recovery;
(4) optionally dissolving or dilution.
2. preparation method as claimed in claim 1, wherein the extraction of step (1) is selected from immersion, decoction, backflow, percolation or its combination.
3. preparation method as claimed in claim 1, wherein used solvent is selected from water, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1 in the step (4), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
4. the preparation method of a Herb Gynostemmae Pentaphylli extract comprises:
(1) solvent extraction raw material, described solvent are selected from methanol, ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain residue;
(3) residue of water extraction step (2).
5. preparation method as claimed in claim 4, wherein the extraction of step (1) and (3) is independently selected from immersion, decoction, backflow, percolation or its combination.
6. as claim 4 or 5 described preparation methoies, also comprise:
(4) separate aqueous extract and residue, obtain aqueous extract;
(5) concentrate, the ethanol precipitate with ethanol separates obtaining precipitation;
(6) alternatively, the resolution of precipitate with step (5) is mixed with solution.
7. preparation method as claimed in claim 6, wherein used solvent is selected from ethanol, isopropyl alcohol, 1-butanols, ethylene glycol, 1 in the step (6), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
8. Herb Gynostemmae Pentaphylli extract, it makes according to any one described preparation method of claim 1-7.
9. Herb Gynostemmae Pentaphylli extract as claimed in claim 8, wherein, the form of described Herb Gynostemmae Pentaphylli extract is selected from solution, powder, ointment, membrane or cream frost.
10. claim 8 or 9 described Herb Gynostemmae Pentaphylli extracts are in the cosmetics of preparation anti-light aging or the purposes in the medicine.
11. the cosmetics of an anti-light aging or medicine wherein contain claim 8 or 9 described Herb Gynostemmae Pentaphylli extracts.
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Cited By (6)
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| CN104771349A (en) * | 2015-03-09 | 2015-07-15 | 胡建华 | Skin care product containing plant extract and preparation method thereof |
| CN105963188A (en) * | 2016-06-15 | 2016-09-28 | 广东科玮生物技术股份有限公司 | Allergy-relieving moistening toner containing gynostemma saponin and preparation method thereof |
| CN106690244A (en) * | 2015-11-14 | 2017-05-24 | 黄志萍 | Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener |
| CN107158054A (en) * | 2017-06-08 | 2017-09-15 | 广州中医药大学 | Small-leaf bonesetting extract as neuroprotective agent application |
| CN109481366A (en) * | 2018-12-17 | 2019-03-19 | 温州古木生物科技有限公司 | It is a kind of to have effects that repair the Chinese medicine composition of skin sunburn and its water extract and fermentation material and application |
| CN111773123A (en) * | 2020-08-12 | 2020-10-16 | 四川碧康生物科技有限公司 | Anti-wrinkle skin care product containing gynostemma pentaphylla extract and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104771349A (en) * | 2015-03-09 | 2015-07-15 | 胡建华 | Skin care product containing plant extract and preparation method thereof |
| CN106690244A (en) * | 2015-11-14 | 2017-05-24 | 黄志萍 | Preparation method and application of novel sweet Gynostemma pentaphyllum Makino. sweetener |
| CN106690244B (en) * | 2015-11-14 | 2019-12-24 | 黄志萍 | Preparation and application of novel sweet gynostemma pentaphylla sweetener |
| CN105963188A (en) * | 2016-06-15 | 2016-09-28 | 广东科玮生物技术股份有限公司 | Allergy-relieving moistening toner containing gynostemma saponin and preparation method thereof |
| CN105963188B (en) * | 2016-06-15 | 2019-02-12 | 广东科玮生物技术股份有限公司 | A kind of Shu Min moisture retention water and preparation method thereof containing gypenoside |
| CN107158054A (en) * | 2017-06-08 | 2017-09-15 | 广州中医药大学 | Small-leaf bonesetting extract as neuroprotective agent application |
| CN107158054B (en) * | 2017-06-08 | 2020-08-28 | 广州中医药大学 | Application of Adhatoda oleracea extract as neuroprotective agent |
| CN109481366A (en) * | 2018-12-17 | 2019-03-19 | 温州古木生物科技有限公司 | It is a kind of to have effects that repair the Chinese medicine composition of skin sunburn and its water extract and fermentation material and application |
| CN111773123A (en) * | 2020-08-12 | 2020-10-16 | 四川碧康生物科技有限公司 | Anti-wrinkle skin care product containing gynostemma pentaphylla extract and preparation method thereof |
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Application publication date: 20130710 |