KR102324672B1 - Composition comprising extract from liriope platyphylla and natural extract - Google Patents

Abstract

The present invention is maekmundong extract; And white jain, manhyeongja, hyangbuja, chrysanthemum, sangbaekpi, cheongung, baekji, sesin, chrysanthemum, orchid, ginger and ginseng; it relates to a composition containing as an active ingredient any one or more natural extracts selected from the group consisting of.

Description

Composition containing maekmundong extract and natural extract as active ingredients {COMPOSITION COMPRISING EXTRACT FROM LIRIOPE PLATYPHYLLA AND NATURAL EXTRACT}

The present invention relates to a composition comprising a maekmundong extract and a natural extract as active ingredients.

Regarding the cause of hair loss, the theory of the action of male hormones in organs such as the hair root and sebaceous glands, the decrease in blood flow in the hair follicle, the scalp abnormality due to dandruff growth or excessive sebum secretion, genetic factors, and aging have been discussed. However, the clear cause of hair loss has not yet been identified, and recently, more and more people are suffering from alopecia, and the age group is also decreasing.

The human body has more than 1.3 million hairs and more than 100,000 hairs. These hairs go through three phases of anagen, catagen, and telogen, and each hair operates in an independent cycle. Hair loss refers to a state in which there is less or no hair than normal due to some cause, and it is largely divided into a general hair loss phenomenon (telogen effluvium), in which hair falls out in the resting state (telogen effluvium), and an abnormal hair loss phenomenon, anagen effluvium. It is possible, but the latter is commonly referred to as hair loss. Hair loss (or hair loss disease) is, depending on the type, symptom, or cause, alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, Traumatic, Trichoti lomania, Pressure Alopecia), etc., are classified into anaphylactic, syphilis (Alopecia syphlltiac), seborrheic alopecia (Alopecia seborrhecia), symptomatic, scarring, and congenital alopecia.

As a conventional method for treating alopecia, there are preparations mainly based on female hormones in relation to hormone theory, but there are reports of not only effects, but also skin inflammation, side effects caused by hormone administration, etc., so their use is currently discontinued. As a hair growth agent, preparations containing minoxidil of Upzon of the United States and trichosaccharide of Trichosaccharide of Crinos Co. of Italy are used as main ingredients, but the absence of a clear effect and the problem of side effects are emerging. In addition, finasteride developed by Merck is known to inhibit the activity of 5-α-reductase, an enzyme that acts on the male hormone testosterone metabolism in hair follicles, but it can cause depression. This also has the problem of requiring long-term treatment. In addition to these, there are Crescina and Rogaine, but they are used only when hair roots are alive on the normal scalp before the hair loss disease has completely progressed. As such, various types of conventional hair regenerative agents have been attempted to help hair growth for the purpose of promoting blood circulation to the scalp and supplying nutrients to the hair roots, but these also have severe toxicity and side effects, and the effect is insufficient. .

On the other hand, although maekmundong is a plant of the Liliaceae family, some efficacy for hair loss improvement is known, but a composition that can show a visible effect on hair loss has not yet been specifically known, and a composition that can exhibit other effects other than hair loss improvement is also Nothing concrete is known yet.

The present invention provides a composition that is superior in preventing hair loss and improving scalp, has excellent anti-dandruff effect, has excellent anti-inflammatory effect, has excellent antibacterial effect, and has excellent sebum suppression effect, than the conventional composition containing the extract of maekmundong. want to

The present invention is maekmundong extract; And white jain, manhyeongja, hyangbuja, chrysanthemum, sangbaekpi, cheongung, baekji, sesin, chrysanthemum, orchid, ginger and ginseng, any one or more natural extracts selected from the group consisting of; provides a composition containing as an active ingredient.

The cosmetic composition according to the present invention contains a natural extract of a previously unknown unique composition along with the extract of maekmundong, and thus has excellent hair loss prevention and scalp improvement effects, excellent anti-dandruff effect, excellent anti-inflammatory effect, and antibacterial effect. It has excellent sebum suppression effect.

Hereinafter, the composition according to the present invention will be described in detail.

Maekmundong is a plant in the family Liliaceae and its scientific name is Ophiopogon. Japonicus or Liriope platyphylla . Steroidal saponins such as Ophiopogonon AE, Spicatoside AB, and flavonoids such as Ophioponanone CF and Ophiopogonone C are known as ingredients. It has been reported that there are differences in the composition depending on the method.

Chrysanthemum is a plant in the Asteraceae family and its scientific name is Chrysanthemum indicum . Flavonoid compounds such as Linarin and Quercetine and phenolic compounds such as Cynarin are known as ingredients.

Mangyeongja is a plant in the Verbena family and the scientific name is Vitex. trifolia or Vitex rotundifolia . Flavonoids such as Vitexcarpin, Artemetin, and Hesperidin are known as ingredients.

Ginseng is a plant of the Araliaceae family, and its scientific name is This is Panax ginseng . Saponins such as ginsenoside (Ginsenoside Rb1-2) are known as ingredients.

Orchid is a plant in the Asteraceae family and its scientific name is Eclipta. It is prostrata . Saponins such as Eclalbasaponin I-IV, and flavonoids such as Wedelolactone are known as ingredients.

Arborvitae is a plant in the arboraceae family, Thuja Orientalis leaves are known to have excellent antibacterial and anti-inflammatory effects. Hyangbuja is a plant belonging to the genus cyperaceae and its scientific name is Cyperus rotundus , and traditional medicines describe that it has the effect of relieving pain and helping the meridians.

White jain is the leaf of the Arboraceae plant, its scientific name is Thuja. orientalis .

The sagebrush is a plant of the Morus family and its scientific name is Morus. It is alba . Flavonoids such as Mrusin and Kuwanon ER are known as ingredients.

Ginger is a plant in the ginger family and its scientific name is Zingiber. It is officinale . Known ingredients include phenolic compounds such as gingerone and shoagaol.

Cnidaria is a plant of the buttercup family and its scientific name is Cnidium. It is officinale .

In an exemplary embodiment of the present invention, the content of the maekmundong extract is 0.01 wt% to 10 wt%, based on the total weight of the composition. If the content is less than 0.01% by weight, hair loss prevention and scalp improvement effects hardly appear, and if it exceeds 10% by weight, the formulation may be affected.

In one embodiment of the present invention, the content of the natural extract is 0.01 wt% to 10 wt%, based on the total weight of the composition.

In one embodiment of the present invention, the composition is maekmundong; And by adding water to any one or more selected from the group consisting of white jain, manhyeongja, hyangbuja, chrysanthemum, sangbaekpi, cheongung, white paper, sesin, chrysanthemum, ginseng, and hot water extraction at a temperature of 95°C to 100°C. The obtained extract is contained.

In one embodiment of the present invention, the composition is maekmundong; And by adding water to any one or more selected from the group consisting of white ginseng, manhyeongja, hyangbuja, chrysanthemum, sangbaekpi, cheongung, white paper, sesin, chrysanthemum, orchid, ginger, and ginseng at a temperature of 20 ° C. to 25 ° C. by low temperature extraction. The obtained extract is contained.

As the extracts contained in the composition according to the present invention, those obtained by extraction and separation from nature by a method commonly used to extract active ingredients from plants may be used, and 'extract' as defined in the present invention is an appropriate solvent. It is extracted using a hot water extract, polar solvent soluble extract or non-polar solvent soluble extract may all include.

As a suitable solvent for preparing the extract, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, purified water, methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol), alcohols having 1 to 4 carbon atoms including butanol (butanol), acetone (acetone), ether (ether) , benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and various solvents such as cyclohexane (cyclohexane) can be used alone or in combination, but Not limited.

As the extraction method, any one or more methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, compression method, etc. can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. The method for preparing the extract of the present invention may also be used in a conventionally known method.

For example, the extract included in the composition of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying the primary extract extracted by the hot water extraction or solvent extraction method described above. In addition, the first extract was further purified using various chromatography methods such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. you may get

Therefore, in the present invention, the extract is a concept including all extracts, fractions and purified substances obtained in each step of extraction, fractionation or purification, their dilutions, concentrates, or dried substances, and the extract is obtained by mixing separate extracts of each herbal medicine. It may be used, or one prepared by mixing herbal materials and extracting them all at once may be used. In addition, the extract according to the present invention can be dissolved in 1,3-butylene glycol and blended into the composition.

In an exemplary embodiment of the present invention, the composition contains an ethyl acetate fraction separated into an upper layer by adding ethyl acetate to the extract obtained by the hot water extraction or the extract obtained by the low temperature extraction, followed by ethyl acetate fractionation.
In one embodiment of the present invention, the composition contains a butanol fraction separated into an upper layer by adding butanol to the lower layer that is not separated into the ethyl acetate fraction.

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In one embodiment of the present invention, the composition contains the extract obtained by adding ethanol to the extract obtained by the hot water extraction or the extract obtained by the low temperature extraction.

The cosmetic composition according to the present invention is excellent for protecting dermal papilla cells, and the composition is for preventing hair loss or improving the scalp.

In addition, the cosmetic composition according to the present invention is for antioxidants with excellent free radical suppression and active oxygen suppression effects.

In addition, the cosmetic composition according to the present invention is for anti-dandruff excellent in the inhibitory effect on the dandruff causative bacteria.

In addition, the cosmetic composition according to the present invention is an antibacterial composition having an excellent inhibitory effect on bacteria.

In addition, the cosmetic composition according to the present invention is for the suppression of sebum having an excellent effect of inhibiting the production of sebum.

In one embodiment of the present invention, the composition is a cosmetic composition. In the composition, other ingredients may be further added within a range that is acceptable for external application to the skin as a cosmetic.

In one embodiment of the present invention, the composition is a food composition. In the composition, other ingredients may be further added within the range of ingredients acceptable for human ingestion.

In addition, the cosmetic composition according to the present invention is a cosmetic composition for antioxidants having excellent free radical suppression and active oxygen suppression effects.

In addition, the cosmetic composition according to the present invention is a cosmetic composition for improving or preventing skin wrinkles having excellent elastase inhibitory effect, excellent collagen synthesis activity effect, and excellent MMP-1 inhibitory effect.

In addition, the cosmetic composition according to the present invention is an anti-inflammatory cosmetic composition with excellent NO inhibition.

In addition, the cosmetic composition according to the present invention is an anti-aging cosmetic composition having excellent skin moisturizing effect, antioxidant effect, skin wrinkle improvement or prevention effect, and anti-inflammatory effect.

Hereinafter, the present invention will be described in more detail by way of Examples. However, these examples are only intended to describe the present invention in detail, and the scope of the present invention is not limited by these examples.

<Example 1> Preparation of hot water extract
100 g each of Maekmundong, Baekjain, Manhyeongja, Hyangbuja, Gamguk, Sangbaekpi, Cheongung, Baekji, Sesin, Arachnid leaves, Hanryeoncho, Ginger and Ginseng were added to 1L to 2L of purified water, and then refluxed at a temperature of 95°C to 100°C. It was extracted while boiling for 10 to 15 hours under After completion of the extraction, the extract from which the raw material was removed was concentrated with a rotary permeate concentrator and freeze-dried to obtain a solid hot water extract.
<Example 2> Preparation of low-temperature extract
100 g each of maekmundong, white jain, manhyeongja, hyangbuja, gamguk, sangbaekpi, cheongung, baekji, sesin, chrysanthemum, chrysanthemum, ginger and ginseng were added to 1L to 2L of purified water, and then from 20℃ to 25℃ at room temperature for 1 week to It was maintained for 3 weeks to elute the active ingredient. After completion of elution, the eluate from which the raw material was removed was concentrated with a rotary permeate concentrator and freeze-dried to obtain a solid low-temperature extract.
<Example 3> Preparation of ethyl acetate fraction
After adding 1 L to 2 L of purified water to the hot water extract of Example 1, the precipitate formed is filtered off, and ethyl acetate is added thereto, mixed well, left to stand to separate into an upper layer and a lower layer, and after obtaining the upper layer, the rotary pressure reduction Concentrated with a concentrator and freeze-dried to obtain a solid ethyl acetate fraction.
<Example 4> Preparation of butanol fraction
1L to 2L of butanol was added to the lower layer separated by the addition of ethyl acetate in Example 3, mixed well and left to separate into upper and lower layers to obtain an upper layer, concentrated with a rotary vacuum concentrator and freeze-dried to obtain a solid state A butanol fraction was obtained.
<Example 5> Preparation of polysaccharide fraction

The hot water extract of Example 1 was concentrated with a rotary tannery concentrator, and the precipitate formed by adding ethanol to the concentrated solution was vacuum-dried to obtain a solid polysaccharide fraction.

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< Experimental example 1> Measurement of antioxidant efficacy

In order to confirm the antioxidant effect, free radical scavenging test and active oxygen scavenging test were performed. The samples used for the test were samples obtained in Examples 1 to 5, and ascorbic acid having excellent antioxidant effect was used as a positive control in order to compare the antioxidant effect.

The free radical scavenging test uses the stable absorbance of DPPH that shows the maximum absorbance at 540 nm, and as the free radical DPPH is cleared by the sample and becomes a transparent color from purple, as the free radical scavenging rate increases, the absorbance at the 540 nm wavelength It was carried out by the test method by applying the decrease in .

In 1 ml of 0.1 mM DPPH (2,2-diphenyl-1-picryl-hydrazyl radical, Sigma) solution, the samples obtained in Examples 1 to 5 were diluted to an appropriate concentration in methanol, 1 ml was mixed, and the mixture was mixed at 37 ° C. After leaving for 15 minutes in a microplate reader (microplate reader, UVT-06685, Thermo max, USA) was used to measure the absorbance at a wavelength of 540nm.

In the free radical scavenging test, the control was measured in the same manner by adding 1 ml of DPPH and 1 ml of methanol, and 1 ml of methanol and 1 ml of the sample were added to obtain color correction values for the sample and the control, respectively. The free radical scavenging rate was calculated using Equation 1 below, and IC ₅₀ is the concentration of the sample required to remove 50% of free radicals, and the smaller the value, the higher the antioxidant activity.

[Equation 1]

Free radical scavenging rate (%) = 100-((absorbance of sample / absorbance of control) × 100)

The active oxygen scavenging test is by measuring the change in absorbance using the oxidation of nitroblue tetrazolium (NBT) by active oxygen using the generation of active oxygen by the enzymatic reaction of xanthine / xanthine oxidase. It can be seen that the scavenging ability of active oxygen.

Sodium carbonate (Na ₂ CO ₃ ) 2.4 ml, xanthine 0.1 ml, ethylenediamine tetraacetic acid (EDTA) 0.1 ml, bovine serum albumin (BSA, Sigma) 0.1 ml, NBT 0.1 ml and Put 0.1 ml of the sample, voltex it using a vortex mixer (Type 37600 Mixer, USA), and leave it at 25 ° C for 10 minutes, then add 0.1 ml of xanthine oxidase and react at 25 ° C for 20 minutes, 6mM CuCl ₂ was added to stop the reaction, and absorbance was measured at a wavelength of 540 nm using a microplate reader.

The control group in the active oxygen scavenging activity test was measured in the same way by adding tertiary distilled water instead of the sample solution, and adding tertiary distilled water instead of the xanthylase solution to obtain each color correction value for the extracted sample and the control was set. .

The active oxygen scavenging rate was calculated using Equation 2 below, and IC ₅₀ is the concentration of the sample required to remove 50% of active oxygen, and the smaller the value, the higher the antioxidant activity.

[Equation 2]

Reactive oxygen scavenging rate (%) = 100-((absorbance of sample / absorbance of control group) × 100)

The free radical scavenging rate and active oxygen scavenging rate were measured with the samples of Examples 1 to 5, and the measurement results are shown in Table 1 below using L-ascorbic acid as a control.

Sample name Free radical scavenging rate IC ₅₀ (ug/mL) Reactive oxygen scavenging rate IC ₅₀ (ug/mL) L-ascorbic acid 4.85 6.02 Example 1 8.87 7.26 Example 2 5.56 6.88 Example 3 6.05 8.45 Example 4 5.09 6.50 Example 5 5.88 7.11

As shown in Table 1, Examples 1 to 5 according to the present invention compared the antioxidant efficacy of the hot-water extract and the low-temperature extract, it was confirmed that the antioxidant efficacy of the low-temperature extract was superior to that of the hot-water extract, and the antioxidant efficacy of the fraction was the butanol fraction, It was confirmed that the polysaccharide fraction and the ethyl acetate fraction were in order.

< Experimental example 2> dermal papilla cells Protection effectiveness measurement

The dermal papilla cells exist at the base of the hair follicle and are responsible for hair growth and hair follicle cycle regulation by supplying oxygen and nutrients to the cells constituting the hair follicle. Therefore, when the proliferation of dermal papilla cells is promoted, hair becomes healthy, hair growth is promoted, and hair loss can be prevented. Because hair growth proceeds as the epithelial cells surrounding the dermal papilla divide to form a hair shaft, the dermal papilla cells play an important role in regulating the division of epithelial cells. Also, in androgenetic alopecia, the site where male hormones act on hair follicles is also the dermal papilla, and dermal papilla cells play a very important role in hair growth.

Hair is an organ with severe oxidative stress as it requires vigorous cell division for continuous growth. Therefore, the samples of Examples 1 to 5, which can protect the hair dermal papilla cells from oxidative stress, were expected to act effectively in preventing hair loss, and it was evaluated whether each sample could protect the hair dermal papilla cells from oxidative stress.

After inoculating 5,000 dermal papilla cells into 96 wells and culturing for 24 hours, each sample was treated in DMEM medium without fetal bovine serum for 48 hours. After treatment with 1 mM hydrogen peroxide for 2.5 hours, washing and culturing in DMEM medium without fetal bovine serum for 24 hours, the viability was measured with WST-1, and the results are shown in Table 2 below.

Cell viability (%) negative control (H ₂ O ₂ unprocessed) 100 positive control 23.15 Example 1 500ppm 57.47 250ppm 49.85 Example 2 500ppm 71.51 250ppm 51.65 Example 3 500ppm 39.56 250ppm 21.01 Example 4 500ppm 63.55 250ppm 50.24 Example 5 500ppm 60.15 250ppm 41.02

As shown in Table 2, Examples 1 to 5 according to the present invention confirmed that the dermal papilla cell protection efficacy from oxidative stress was excellent, and in particular, Example 4 confirmed that the dermal papilla cell protection efficacy from oxidative stress is excellent.

< Experimental example 3> Measurement of dandruff production inhibitory activity

Dandruff refers to a keratinous mass formed by the dead or exfoliated cells of the scalp caused by the body's metabolism and dried together with the secretions of the scalp sebaceous glands. Dandruff gradually demarcates from normal skin and progresses to seborrheic dermatitis, usually accompanied by itching and inflammation. Therefore, in order to evaluate whether the sample of Examples 1 to 5 inhibits the generation of dandruff, the dandruff inhibitory efficacy was evaluated in anticipation that the substance inhibiting the generation of dandruff would effectively act in preventing hair loss and improving the scalp.

In order to confirm the dandruff generation inhibitory efficacy of each sample, the inhibitory effect was measured against the dandruff causative bacterium Pityrosporum ovlae (ATCC 12087). Each sample is diluted stepwise in the medium using the broth dilution method, and after inoculating the bacteria in this dilution, shake and incubate for 48 hours in an incubator at 35 ° C. was measured, and the results are shown in Table 3 below.

Minimum inhibitory concentration (MIC) Example 1 2.10 Example 2 1.95 Example 3 3.15 Example 4 1.71 Example 5 2.52

As shown in Table 3, Examples 1 to 5 according to the present invention were confirmed to have excellent dandruff production inhibitory activity.

< Experimental example 4> Measurement of anti-inflammatory efficacy

In order to confirm the anti-inflammatory effect of the samples of Examples 1 to 5, RAW264.7 cells, which are macrophages of mice, were purchased from ATCC and used. DMEM/F12 (Dulbecco's modified Eagle's medium/ Nutrient Mixture Ham's F12), FBS (fetal bovine serum), L-glutamine, penicillin-streptomycin and insulin used for cell culture were Gibco/BRL ( USA). The RAW264.7 cells were cultured in DMEM/F12 medium in which 10% FBS, 1% penicillin streptomycin, 1% L-glutamine, and 20 μg/ml insulin were added, and 37° C. wet CO₂ incubator (5 % CO₂/95% air). After the cells were cultured in about 80% of the culture dish cold, the monolayer of cells was washed with PBS (pH 7.4), washed, and subcultured by treatment with 0.25% trypsin and 2.56 mmol/L EDTA.

After adding each sample and LPS together, after culturing for 24 hours, the culture solution after culturing for 24 hours was collected, and the amount of NO secretion was measured using the Griess reagent system (Promega, USA), and the results are shown at the bottom. indicated. The measurement method using the Griess reagent system was, in more detail, according to the protocol provided by Promega, the manufacturer of the system. Sulfanilamide solution was first put into the collected cell culture medium, NED solution was put in, and then a microplate reader was performed by a method of measuring at 540 nm using a , and the results are shown in Table 4 below.

Sample name treatment concentration NO inhibition rate (%) Example 1 500ppm 60.12 250ppm 43.53 Example 2 500ppm 35.94 250ppm 27.84 Example 3 500ppm 39.65 250ppm 17.85 Example 4 500ppm 89.15 250ppm 76.66 Example 5 500ppm 45.11 250ppm 29.56

As shown in Table 4, Examples 1 to 5 according to the present invention were confirmed to have excellent anti-inflammatory activity, in particular, it was confirmed that the excellent anti-inflammatory activity of Example 4.

<Experimental Example 5> Measurement of antibacterial efficacy

As an antibacterial effect test for the samples of Examples 1 to 5, the antibacterial test was performed by the agar serial dilution method and the paper disc method. Experimental strains received pre-sale from the Korea Research Institute of Bioscience and Biotechnology, a Gram-positive staphylococci (Staphylococcus aureus, KCTC 6910), a Gram-negative bacteria Pseudomonas aeruginosa (Pseudomonas aeruginosa, KCTC 1637), Escherichia coli (E. coli, KCTC 1039), a yeast Candida Yeast ( Candida albicans , KCTC 7965 ) and filamentous fungi were used as a total of 5 strains of Heukgukgyun ( Aspergillus niger , KCTC 6910 ) or more.

The bacteria were inoculated in the tryptic soy medium and pre-cultured at 37°C for 24 hours, and in the case of yeast, the bacteria were inoculated in a potato dextrose medium and pre-cultured at 25°C for 2 days, and the filamentous bacteria were grown on a potato dextrose agar medium. After inoculating the bacteria and pre-culturing for 7 days in an incubator at 25° C., spores of filamentous fungi formed on the surface of the medium were recovered using a smear and diluted in sterile saline for use.

In a sterile Petri dish, 2 ml of each sample (Examples 1 to 4) diluted to an appropriate concentration in 5% dimethyl sulfoxide (DMSO)-containing physiological saline solution for each experimental strain was added, and 5% DMSO-containing physiological saline solution was added. After adding 2 ml each as a control sample, 18 ml of tryptic soy agar medium and potato dextrose agar medium sterilized and cooled to 48° C. were added to each Petri dish, stirred, and then allowed to solidify.

Afterwards, each pre-cultured test bacteria ^{is smeared on each Petri dish at a final concentration of about 1 X 10 6} CFU/ml for bacteria, 1 X 10 ⁵ CFU/ml for yeast, and about 1 for filamentous bacteria X 10 ⁴ Smear on each Petri dish at a bacterial concentration of CFU/ml. In each Petri dish, bacteria were cultured at 37°C for 24 hours, yeast was cultured at 25°C for 3 days, and filamentous fungi were cultured for 7 days in an incubator at 25°C, and colony formation was observed in each compartment.

The minimum sample concentration of the non-growing plate is the minimum inhibitory concentration (MIC), and the smaller the minimum inhibitory concentration, the higher the antibacterial effect.

The results of the antimicrobial activity test are shown in Table 5 below.

sample Minimum inhibitory concentration (MIC) S. aureus P. aeruginosa E. coli C. albicans A. niger Example 1 0.5 0.5 0.3 0.3 0.6 Example 2 0.6 0.7 1.1 1.0 0.5 Example 3 0.3 0.3 0.6 0.5 0.5 Example 4 0.2 0.3 0.2 0.2 0.1 Example 5 0.3 0.4 0.2 0.2 0.2

As shown in Table 5, Examples 1 to 5 according to the present invention were confirmed to have excellent antibacterial activity.

< Experimental example 6> sebum secretion Determination of Inhibitory Efficacy

Human sebaceous gland cells were obtained from hair follicles in the dermal layer, and the isolated sebaceous gland cells were treated with 0.25% Trypsin and 0.05% Ethylene Diamene Tetraacetic Acid (EDTA) for 30 minutes. After treatment at ℃, trypsin inactivation with MEM (Eagle's minimum essential medium) containing 12% fetal bovine serum (FBS), the medium was removed by centrifugation, and then KGM (keratinocyte growth medium) and cultured in a 60 mm culture dish. For this experiment during subculture, 2X10 ⁵ cells are planted per well using a 24-well-plate. After the cells were cultured for 24 hours, the samples of Examples 1 to 5 were dispersed in dimethylsulfoxide (DMSO) in each well, treated with a final concentration of 0.1% (w/v), and cultured for 72 hours. Thereafter, the number of surviving cells was counted, and the amount of sebum generated was extracted and measured using methanol:chloroform extraction, and ^{the total amount of sebum generated per 10 4} cells was compared to compare the degree of inhibition of sebum production. The extraction method of sebum was the method of Zoboulis et. al. (J. Invest. Dermatol., 1991; 24, 69-83), methanol: chloroform extraction step, ultrasonic crushing step, filtering step , including a nitrogen evaporation step. Table 6 shows the results of this experiment.

Sebum production inhibition rate (%) Example 1 38 Example 2 22 Example 3 31 Example 4 48 Example 5 41

As shown in Table 6, Examples 1 to 5 according to the present invention were confirmed to have excellent sebum production inhibitory activity.

< Experimental example 7> Protein content analysis

In order to measure the protein content of each sample (Examples 1 to 5), a bicinchoninic acid (BCA) measurement method was performed. The BCA measurement method uses ^{the property of a protein to reduce copper ions (Cu 2+} , Cu ¹⁺ ). The copper ions reduced by the protein react with the BCA solution to form a purple compound (Cu ⁺ /BCA chromophore). This purple compound shows the maximum absorbance at a specific wavelength of 562 nm. Therefore, as the amount of protein increased, more purple compounds were formed, and the amount of protein was measured using the increase in absorbance at 562 nm. The experimental method is as follows.

First, using a micro BCA protein measurement reagent kit (Pierce, cat. no. 23227), put 150 μl of standard solution or diluted sample into each 96 microplate well, and then add BCA (bicinchoninic acid) kit WR (Working Reagent) reagent. was added and mixed vigorously for 30 seconds. Thereafter, the reaction was carried out at 37° C. for 2 hours, and absorbance at 540 nm was measured with a microplate reader (UVT-06685, Thermo max, USA) to prepare a calibration curve and protein quantification. The result is as follows.

Sample name Total protein content (%) Example 1 6.25 Example 2 5.10 Example 3 3.15 Example 4 7.51 Example 5 7.36

< Experimental example 8> Analysis of constituent amino acids

0.2 g of each sample (Examples 1 to 5) was hydrolyzed in 20 ml of 6N HCl at 130° C. for 24 hours to break the peptide bond of the protein to obtain free amino acids. After that, the free amino acid was formed using a derivative of OPA (o-phthalaldehyde, Agilent) and FMOC (9-fluorenylmethylchloroformate) to form a fluorescent isoindole, followed by high-performance liquid chromatography (Agilent Technologies 1200 Series HPLC, Agilent, USA). ) using a fluorescence detector (FLD, Agilent Technologies, USA) to detect amino acids. As a standard material, the component amino acids of the 250pM composition were identified. The content is shown in Table 8 below.

Amino
acid
(ppm) Asp Glu Ser His Gly The Arg Ala Tyr Cys Val Pro line
city
Yes One 2.21 0.85 0.71 1.21 - - 1.27 0.68 0.32 1.64 1.10 1.92 2 - 1.15 0.23 - 0.21 0.08 0.56 0.25 - 0.98 0.05 - 3 2.73 0.29 0.89 0.44 0.10 0.02 0.24 1.13 - - 0.66 - 4 0.35 - - 1.02 0.34 - - - 0.10 1.22 0.25 0.86 5 0.71 0.16 0.15 0.89 0.18 - 1.02 0.60 0.52 0.56 1.12 1.56

<Formulation Preparation Example> Formulation example 1 to 3 and Comparative formulation example Preparation of 1 to 3

In order to check the feeling of use according to the formulation of the composition according to the present invention, an anti-hair loss hair shampoo composition according to the composition of Table 9 (Formulation Example 1), and an anti-hair loss hair conditioner composition according to the composition of Table 10 (Formulation Example 2) And an anti-hair loss hair tonic composition (Formulation Example 3) according to the composition of Table 11 was prepared.

In addition, in order to compare with the formulation example according to the present invention, according to the composition of Table 12

A general shampoo composition (Comparative Formulation Example 1), a general anti-hair loss hair conditioner composition according to the composition of Table 13 (Comparative Formulation Example 2) and a general anti-hair loss hair tonic composition according to the composition of Table 14 (Comparative Formulation Example 3) prepared.

number Raw material content (wt%) One Polyquaternium-10 0.2 2 Sodium Lauryl Sulfate 15 3 Sodium Laureth Sulfate 45 4 glycol stearate 2 5 cetearyl alcohol 0.5 6 Lauryldiethylamide 2.5 7 Composition of Example 4 1.0 8 Phenoxyethanol 0.7 9 Purified water 33.1

number Raw material content (wt%) One cetyl alcohol 3.0 2 stearyl alcohol 3.0 3 propylene glycol 2.0 4 cetyltrimethylammonium chloride 1.0 5 poly dimethylsiloxane 1.0 6 Composition of Example 4 1.0 7 mineral oil 0.5 8 citric acid 0.2 9 Phenoxyethanol 0.7 10 Purified water 87.6

number Raw material content (wt%) One menthol 0.3 2 ethanol 30 3 salicylic acid 0.1 4 propylene glycol 0.25 5 triethanolamine 0.2 6 panthenol 0.1 7 Phenoxyethanol 0.7 8 Purified water 68.35

number Raw material content (wt%) One Polyquaternium-10 0.2 2 Sodium Lauryl Sulfate 15 3 Sodium Laureth Sulfate 45 4 glycol stearate 2 5 cetearyl alcohol 0.5 6 Lauryldiethylamide 2.5 7 Purified water 34.8

number Raw material content (wt%) One cetyl alcohol 3.0 2 stearyl alcohol 3.0 3 propylene glycol 2.0 4 cetyltrimethylammonium chloride 1.0 5 poly dimethylsiloxane 1.0 6 mineral oil 0.5 7 citric acid 0.2 8 Phenoxyethanol 0.7 9 Purified water 88.6

number Raw material content (wt%) One menthol 0.3 2 ethanol 30 3 salicylic acid 0.1 4 propylene glycol 0.25 5 triethanolamine 0.2 6 panthenol 0.1 7 Phenoxyethanol 0.7 8 Purified water 68.35

< Experimental example 9> Shampoo formulation feeling test

30 university students were divided into two groups, and a feeling test was conducted on the formulation for 4 weeks. The product was to be used at least 3 times a week, and a survey was conducted after use for a total of 6 weeks. The survey scale was conducted as follows.

5 points: very good/ 4 points: good/ 3 points: average/ 2 points: no effect/ 1 point: poor

The results of the questionnaire after the comparative usability test for Formulation Examples 1 to 3 according to the present invention and Comparative Formulation Examples 1 to 3 corresponding thereto are shown in Tables 15 to 17 below.

As a result of the survey, it was confirmed that the formulation according to the present invention received a higher score than the formulation in which the composition according to the present invention was not added.

Questionnaire Formulation Example 1 Comparative Formulation Example 1 1. The itching of the scalp was reduced. 3.8 3.2 2. Dandruff decreased. 3.6 3.3 3. The sebum of the scalp decreased. 3.2 3.1 4. The thickness of the hair has become thicker. 3.0 2.9 5. After shampooing, hair feels shiny 4.0 3.5 6. When rinsing, the finger passes well. 3.2 3.1 7. After drying, the hair is easy to care for and soft. 3.4 3.1 8. Moisture of hair lasts long after shampooing 3.7 3.5

Questionnaire Formulation Example 2 Comparative Formulation Example 2 1. The itching of the scalp was reduced. 3.3 3.2 2. Dandruff decreased. 3.4 3.1 3. The sebum of the scalp decreased. 3.2 3.2 4. The thickness of the hair has become thicker. 3.1 3.0 5. After using conditioner, hair feels shiny 3.8 3.6 6. When rinsing, the finger passes well. 3.6 3.1 7. After drying, the hair is easy to care for and soft. 3.7 3.2 8. Moisture of hair lasts long after conditioner 3.5 3.2

Questionnaire Formulation Example 3 Comparative Formulation Example 3 1. The itching of the scalp was reduced. 3.8 3.5 2. Dandruff decreased. 3.6 3.3 3. The sebum of the scalp decreased. 3.5 3.3 4. The thickness of the hair has become thicker. 3.5 3.2 5. After shampooing, you can feel the luster of your hair 3.7 3.5

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, these specific descriptions are only preferred embodiments, and it is clear that the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

Claims (16)

A cosmetic composition comprising, as an active ingredient, a mixed extract of maekmundong extract, white jasmine extract, manhyeongja extract, hyangbuja extract, chrysanthemum extract, chrysanthemum extract, cheongung extract, white paper extract, sessin extract, arborvitae extract, orchid extract, ginger extract and ginseng extract as,
The mixed extract is a hot water extract obtained by adding water to maekmundong, white jain, manhyeongja, hyangbuja, gamguk, sangbaekpi, cheongung, white paper, sesin, chrysanthemum, chrysanthemum, ginger and ginseng, followed by extraction at a temperature of 95°C to 100°C. A cosmetic composition, characterized in that it is a butanol fraction separated by adding ethyl acetate to remove the ethyl acetate fraction, and then adding butanol to the fraction not separated into the ethyl acetate fraction. According to claim 1,
The content of the maekmundong extract is, with respect to the total weight of the composition, a cosmetic composition, characterized in that 0.01% to 10% by weight. According to claim 1,
The total content of the white ginseng extract, manhyeongja extract, hyangbuja extract, chrysanthemum extract, sangbaekpi extract, cheongung extract, white paper extract, sesin extract, arborvitae extract, orchid extract, ginger extract, and ginseng extract is 0.01 weight based on the total weight of the composition % to 10% by weight, the cosmetic composition. delete delete delete delete delete According to claim 1,
The composition is a cosmetic composition, characterized in that for preventing hair loss or improving the scalp. According to claim 1,
The composition is characterized in that for antioxidant, cosmetic composition. According to claim 1,
The composition is characterized in that for anti-dandruff, cosmetic composition. According to claim 1,
The composition is characterized in that for antibacterial, cosmetic composition. According to claim 1,
The composition is a cosmetic composition, characterized in that for inhibiting sebum secretion. According to claim 1,
The composition is characterized in that for anti-inflammatory, cosmetic composition. According to claim 1,
The mixed extract is a mixture consisting of white ginseng extract, manhyeongja extract, hyangbuja extract, chrysanthemum extract, sangbaekpi extract, cheongung extract, white paper extract, sessin extract, arborvitae extract, chrysanthemum extract, ginger extract and ginseng extract and maekmundong extract 12:1 A cosmetic composition, characterized in that it is mixed in a weight ratio of According to claim 1,
The weight ratio of the maekmundong extract, white ginseng extract, manhyeongja extract, hyangbuja extract, chrysanthemum extract, sangbaekpi extract, cheongung extract, white paper extract, sesin extract, arborvitae extract, chrysanthemum extract, ginger extract and ginseng extract is 1:1:1:1 1:1:1:1:1:1:1:1:1:1, a cosmetic composition.