KR100941133B1 - Cosmetic composition - Google Patents

Abstract

PURPOSE: A cosmetic composition containing antiseptic substitute and natural plant extract is provided to ensure excellent antibacterial activity and prevent skin irritation with formulation stability. CONSTITUTION: A cosmetic composition contains 0.1-1.0 weight% of ethylhexylglycerin, 0.1-1.0 weight% of caprylic glycol, 0.001-10.0 weight% of Needle Juniper extract, 0.001-10.0 weight% of chives extract, and 0.001-10.0 weight% of Mosla japonica extract. The carpylic glycol and ethyl hexane glycerin is mixed in the ratio of 10:1-1:10. The extract is isolated using water, anhydrous or hydrous alcohol of 1-4 carbon atoms, acetone, ethyl acetate, chloroform or 1,3-butyleneglycol. The cosmetic composition is used for anti-inflammation and relieving skin irritation. The cosmetic composition is used in the form of emulsion, cream, gel, pack, cleansing cream, foam cleansing, foundation, mascara, eye shadow or eye liner.

Description

Caprylyl glycol ethylhexylglycerin and juniper extract leek extract and wild berry extract having antiseptic and anti-inflammatory activity and a cosmetic composition containing the same as an active ingredient {Cosmetic Composition}

The present invention relates to a cosmetic preservative substitute and an antiseptic cosmetic composition using the same, and more particularly, do not mix the ingredients (labeled designated ingredients) legally designated as a disinfectant and preservative in the existing cosmetic method, it is safe to the skin and antibacterial effect Eggplant relates to a preservative-free cosmetic composition using caprylyl glycol and echylhexylglycerin and juniper extract, leek (Allium tuberosum) extract and Mosla japonica extract, and used in cosmetics.

In general, since the cosmetic composition is composed of various raw materials such as cosmetic oil, surfactant, inorganic powder, organic water-soluble polymer, and fragrance, it contains a large amount of raw material which is a carbon source of microorganisms, and secondary contamination by repeated use is concerned. In addition, unlike foods and pharmaceuticals, the addition of preservatives was necessary because of contamination caused by long-term use.

Preservatives are used for the purpose of preventing the change of products by inhibiting the growth of microorganisms that are contaminated with cosmetics from the outside. Preservatives are currently used in cosmetics such as paraoxybenzoic acid esters and phenoxy. Ethanol, imidazolidinyl urea, DMDM hydantoin, benzalkonium chloride and the like are generally used. Recently, as new additives such as proteins, gums, vitamins, and medicinal plants are added, changes caused by the action of microorganisms are also diversified, and thus the amount of preservatives added is increased due to the weakening of preservative function.

Of course, it is also possible to produce a cosmetic composition that does not contain preservatives, but in this case, complex means, such as aseptic manufacturing, limiting the amount or duration of use, or specially manufacturing containers, are required to secure antiseptic power. There is a high tendency to lack.

In addition, many extracts having antimicrobial effects have been studied, but the extract alone has the same effect as preservatives, and at the same time, there are many limitations in securing skin safety and formulation stability. In detail, the grapefruit seed extract (Grapefruit Seed extract) shows a small amount of antiseptic effect, but it has a high irritation to the skin, which limits its use. There is a disadvantage that affects the stability of the. In addition, many extracts have been studied, but the amount of use is excessive, or there are many restrictions such as discoloration and odor. Due to these problems, research on natural preservatives with excellent safety and economical efficiency is ongoing, but most of the reported natural antimicrobial substances are not commercialized due to color taste, poor safety, narrow antimicrobial spectrum, and formulation problems. Only some extracts such as hinokitiol, magnolia extract, megnonol, and grapefruit extract DF-100 have been used in consideration of various restrictions.

Therefore, there is an urgent need for the development of a preservative having sufficient preservative power without any side effects on the skin.

The present invention has no relation to the disinfectant or preservative ingredients (labeled designated ingredients) legally designated by the existing cosmetic law, and is a juvenile rigida extract and caprylyl extract as a preservative ingredient for cosmetics having a safe and antibacterial effect on the skin. A special formulation of lycol (Caprylyl Glycol) and Ethylhexylglycerin (Ethylhexylglycerin) is used as a preservative substitute, characterized in that to produce a stable preservative-free cosmetic composition by using the preservative substitute in place of conventional preservatives.

In general, the Food and Drug Administration may make microbial contamination easily in the case of cosmetics unless the ingredients specified as sterilization and preservatives are legally designated and labeled as designated ingredients, but the preservative-free cosmetics according to the present invention Will have the preservative effect of the conventional cosmetics without using the designated sterilization or preservatives described above.

In the present invention, the antimicrobial activity of the various raw materials expected to have an antimicrobial effect used in cosmetics to test the effect of killing microorganisms, based on the results obtained from the juniper, juniper extract, caprylyl glycol and ethyl Hexyl glycerine was selected and then applied to cosmetics to measure the killing effect on microorganisms such as bacteria, fungi and bacteria through the CTFA microbial test method. The present invention was completed by demonstrating that the antiseptic effect was excellent when the extract, caprylyl glycol and ethylhexylglycerin were combined.

At this time, as a raw material having an antimicrobial effect used in the present invention, juniper extract, caprylyl glycol and ethylhexylglycerin, the double juniper (Juniperus rigida Siebold & Zucc.) Extracts of juniper trees native to Korea Obtained from leaves, berries, stems, etc. Juniper tree is a evergreen needle leaf tree belonging to the genus Juniperus rigida, and in Korea, juniper (cypress), cedar (sitting juniper), cypress, cedar, snow juniper, cedar, and juniper (juniper) , Beach juniper, and cedar are growing. Juniper fruits produce dark red fruit and are called dusongsil. In North Korea's synonym, it has been documented that it eliminates the customs and has diuretic effect on the ducal cell, and it has the effect of falsification, expectoration, and fungi in pharmacological experiments, edema, diseases of the bladder and urethra, rheumatoid arthritis, amoeba Fruits contain 0,5 ~ 2% essential oils, 40% sugar and 0,9% rosin.

Also, leek (Allium tuberosum) is a lily and perennial plant called root, bulbous seeds, mouth mouth. These outposts are known to be effective in the treatment of bruises, insects, wounds, itching. Another name is perennial herb of lily family, also known as tennis, sorghum, weed and weed, and scaly stem is used for dry stomach, suit and burn, and seeds are used in herbal medicine as urinary medicine. Soft plants are edible. The sites used according to the invention are leaves and stems. Leek (Allium tuberosum) has a higher content of beta-carotene, especially than other plants, and contains antioxidant chlorophyll, glutathione, and allicin to aid in the absorption of vitamin B1. Leek protects the liver and kidneys, so the nourishing tonic effect is clear, it clears the blood and energizes the cells. Diuretic effect, the effect of the branch, etc. It is effective in digestion and gastroenteritis by enhancing the secretion of digestive fluids. Nosebleeds, postpartum bleeding also has a hemostatic effect. It is also effective for bloody blemishes with blackened faces.

Mosla japonica is a perennial plant of the dicotyledonous plant Lamiaceae, which grows on both sides of the mountains and fields. The stems are straight, square and branched. Fragrances throughout the abandonment and hairy on the nodes. Petioles are short, serrated at the edges, and the sunlit part is purple. Flowers bloom in pale reddish purple in July-August and run in inflorescences at the end of stems or branches.

The whole aeration is used as a raw material for thymol. Distributed in Korea and Japan. Blue vesicles (var. Robusta) are entirely green and larger. The outpost of the wild sesame is called Seok-Jin-Ning, and it is medicinal and contains alkaloid, saponinm tannin, l-thujine, d-sabinene, d-limonene, a-caryphyllene, phellandrene, etc. Efficacy and hemorrhage, chronic bronchitis, rubella, dermatitis is effective.

Caprylyl glycol is an INCI name, Caprylyl Glycol, which acts as a moisturizer with antimicrobial action.It is a composition that can exhibit sufficient antiseptic effect or greatly reduce the amount of chemical preservatives even when used alone as one of the emollients. In addition, it has been tested to adversely affect the stability of the formulation when used in large quantities. Ethylhexylglycerin is an Ethylhexylglycerin INCI Name. It has antibacterial effect and various skin control functions.

The present invention has been made to solve the above problems, the object of the present invention is to overcome the problem of causing skin irritation of the chemical preservatives added to cosmetics, caprylyl glycol and ethyl hexyl glycerin of the various cosmetics When mixed in the mixture, it exhibits sufficient antiseptic effect, alleviates the skin irritation caused by caprylyl glycol and ethylhexyl glycerin, and extracts juniperus rigida, allium tuberosum, and wild berry which have anti-inflammatory activity. Mosla japonica) is a composition containing an extract that is excellent in the stability of the formulation to provide a cosmetic composition that can be usefully added to the cosmetic composition. In order to achieve the above object, the present invention is a composition containing 0.1 to 1.0% by weight of caprylyl glycol and 0.1 to 1.0% by weight of ethylhexylglycerin based on the total weight of the cosmetic composition and juniper extract 0.001-10 It provides a cosmetic preservative substitute comprising a weight%, leek extract 0.001 ~ 10% by weight, mountain persimmon extract 0.001 ~ 10% by weight and a composition containing the same as an active ingredient.

Moreover, in order to achieve another objective, this invention provides the antiseptic preservative cosmetic composition containing the said antiseptic composition.

The present invention relates to a cosmetic composition containing 0.1 to 1.0% by weight of caprylyl glycol, ethylhexylglycerin and 0.01 to 10.0% by weight of juniper extract, leek extract, and wild sesame extract as preservatives. It is about.

Thus, the present inventors conducted a study to overcome the above problems, as a result of juvenile extract, leek extract and wild jasmine extract and caprylyl glycol, ethylhexyl glycerine in the cosmetic composition to provide sufficient antiseptic effect The present invention was completed by confirming that it hardly induces skin irritation, has anti-inflammatory activity, excellent stability of the formulation, and can be usefully added to cosmetic compositions.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing juniper extract, leek extract and wild berry extract, caprylyl glycol, and ethylhexylglycerine as preservatives.

In order to achieve the above object, the present invention is 0.1 to 1.0% by weight of caprylyl glycol and 0.1 to 1.0% by weight of ethylhexylglycerin, 0.001 to 10% by weight of juniper extract, leek It provides a cosmetic preservative substitute comprising 0.001 to 10% by weight of the extract, 0.001 to 10% by weight of the wild sesame extract.

In order to achieve another object, the present invention provides a cosmetic composition for reducing skin irritation and anti-inflammatory containing the antiseptic composition.

Moreover, in order to achieve another objective, this invention provides the antiseptic preservative cosmetic composition containing the said antiseptic composition.

Hereinafter, the present invention will be described in detail.

At this time, if there is no other definition in the technical terms and scientific terms used, it has a meaning commonly understood by those of ordinary skill in the art.

In addition, the cosmetic composition according to the present invention is 0.1 to 1.0% by weight of caprylyl glycol and 0.1 to 1.0% by weight of ethylhexylglycerol, based on the total weight of the cosmetic composition as a raw material having an antimicrobial effect in order to obtain a desired antiseptic effect, Juniper extract 0.001 to 10% by weight, leek extract 0.001 to 10% by weight, is characterized in that it is used in an amount of 0.001 to 10% by weight of the wild berry extract. If the amount of caprylyl glycol and ethylhexylglycerin is less than 0.1% by weight, the antiseptic effect is reduced. If the content is more than 1.0% by weight, the synergistic effect of the antiseptic effect is insignificant compared to the increased amount, and the amount of other components is used. This is because there is a problem that is reduced. In addition, juniper extract, leek extract and wild jasmine extract is less than 0.001% by weight of the skin irritation effect and anti-inflammatory activity is reduced, when exceeding 10% by weight of the skin irritation effect and anti-inflammatory activity is insignificant compared to the increased amount This is because there is a problem that the amount of use of other components is reduced.

Caprylyl glycol and ethylhexylglycerin which can be used in the cosmetic composition and the preservative of the present invention are chemically synthesized. In a preferred embodiment of the present invention, caprylyl glycerine from drstraetmans and ethylhexylglycerine from Adeka are used. It is not necessarily limited to those manufactured by the manufacturer.

Juniper extract, leek extract, and perilla extract in the present invention can be obtained through various methods known in the art, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol, Butanol, etc.), acetone, ethyl acetate, chloroform or 1,3-butylene glycol were obtained as an extraction solvent. Preferred extraction solvents are water, ethanol or methanol, more preferably a mixture of water and ethanol, most preferably 70% ethanol. Suitable amounts of the extraction solvent are 3 to 10 times dry weight, preferably 3 to 8 times and most preferably 5 times.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained in addition to the above-described extraction solvents, extracts having substantially the same effect using other extraction solvents.

The extract of the present invention is prepared by a process comprising the following steps: (a) water of 3 to 10 times the dry weight of natural products of juniper, leek, berry, leaves, stems, etc. Adding a solvent selected from the group consisting of four anhydrous or hydrous lower alcohols, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol; (b) refluxing the solvent-added natural product of step (a) to cold extraction for 1 to 7 days or to heat extraction for 4 to 12 hours; And (c) filtering and drying or lyophilizing the extracted components of step (b).

The extract of the present invention includes not only the extract by the aforementioned extraction method, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the present invention.

Juniper extract, leek extract, and perilla extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

In a preferred embodiment of the present invention, the content of the extract is 0.001 to 10.0% by weight, preferably 0.1 to 2% by weight, based on the total cosmetic composition. When the content of caprylyl glycol, ethylhexylglycerin and juniper extract, leek extract, and perilla extract is less than 0.1% and 0.001% by weight, the antibacterial effect, especially antiseptic and skin irritation and anti-inflammatory effect Unexpectedly, and if their content exceeds 10.0% by weight, the synergistic effect is insignificant, and the formulation is not stable, which is not preferable as a cosmetic composition.

Caprylyl glycol and ethylhexylglycerol contained in the cosmetic composition of the present invention is preferably mixed in a ratio of 10: 1 to 1:10, more preferably in a ratio of 5: 1 to 1: 1. The composition is mixed, and most preferably, the composition is at a ratio of 3: 2.

Juniper extract, leek extract, and wild sesame extract contained in the cosmetic composition of the present invention are preferably mixed in a ratio of 10: 10: 1 to 1: 1: 10, and more preferably in a ratio of 1: 1: 1. Mixed composition.

According to a preferred embodiment of the present invention, the mixture of caprylyl glycol, ethylhexylglycerin mixed composition and juniper extract, leek extract, and wild sesame extract is 0.3% by weight and 3.0% by weight, respectively, in the cosmetic composition. Skin irritation relief while showing the effect of caprylyl glycol, ethylhexylglycerine mixed composition and juniper extract, leek extract, and wild sesame extract at 0.3 wt% and 3.0 wt%, respectively. And useful cosmetic compositions having anti-inflammatory activity.

Ingredients contained in the cosmetic composition of the present invention as an antiseptic agent caprylyl glycol, ethyl hexyl glycerin and juniper extract, leek extract, wild berry extract contains an active ingredient that can impart a useful effect to the skin in addition to . Active ingredients that can impart useful effects to the skin include anti-aging ingredients, wrinkle improvement ingredients, whitening ingredients, moisturizing ingredients and antibacterial ingredients, and the like, and are not limited to specific ingredients. In addition to the above components, it may also include components conventionally used in cosmetic compositions, and include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

The compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, when the composition of the present invention is used as a cosmetic composition, a basic cosmetic selected from lotion, emulsion, cream, essence, gel, pack, cleansing cream and foam cleansing; Makeup cosmetics selected from foundation, mascara, eye shadow and eyeliner; Cosmetics for hair of shampoos or rinses; And it can be prepared in a formulation selected from the group consisting of body cosmetics, such as body wash.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol, oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Preparation of Juniper Extract

The fruits, leaves and stems of the juniper are washed, dried and broken up into small pieces, put into 70% aqueous ethanol solution, cold extracted for 72 hours in an extractor and filtered through 400 mesh. After aging for 5 to 10 days, it was filtered through Whatman No. 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C or lower. Juniper extract was prepared by dispersing / dissolving using a vacuum concentrate and a freeze-dried 0.001 to 10.0% by weight of one or more solvents selected from purified water, ethanol, butylene glycol, propylene glycol.

Example 2 leek extract preparation

The dried and dried leek was refluxed three times for 5 hours with a 70% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C or lower. The leek extract was prepared by dispersing / dissolving the depressurized concentrate and the lyophilized product by using at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol.

Example 3 Preparation of Wild Sesame Extract

The dried wild perilla was refluxed three times for 5 hours with an aqueous 70% (V / V) ethanol solution, and the mixture was cooled and then filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C or lower. The dried perilla extract was prepared by dispersing / dissolving the depressurized concentrate and the lyophilized product in one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol.

Example 4 Preparation of Mixed Extract of Juniper Extract, Leek Extract, and Wild Sesame Extract

Juniper extract, leek extract, and wild sesame extract obtained in Examples 1 to 3 are preferably mixed in a ratio of 10: 10: 1 to 1: 1: 10, and more preferably in a ratio of 1: 1: 1. Mixed composition.

Example 5 Preparation of Mixed Extract of Caprylyl Glycol and Ethylhexylglycerine

Caprylyl glycol and ethylhexyl glycerin are preferably mixed in a ratio of 10: 1 to 1:10, more preferably in a ratio of 5: 1 to 1: 1, and most preferably The composition was mixed at a ratio of 3: 2.

Experimental Example 1 Antibacterial Activity of Caprylyl Glycol and Ethyl Hexyl Glycerin

To investigate the antimicrobial activity of caprylyl glycol and ethylhexylglycerin, methylparaben, propylparaben, phenparaethanol, chlorphenesin and caprylyl glycol, and ethylhexylglycerine mixed composition, which are previously used as preservatives Bacteria have antibacterial effects against Escherichia coli ATCC 8739, Pseudomonas aeruginosa (ATCC9027), and Staphylococcus aureus (ATCC6538) and fungi ( C.albicans) . Measured.

A mixed composition of caprylyl glycol and ethylhexylglycerine was prepared by the following method.

Caprylyl glycol and ethylhexylglycerine are preferably mixed in a ratio of 10: 1 to 1:10, more preferably in a ratio of 5: 1 to 1: 1, and in this embodiment, The composition was mixed at a ratio of 3: 2.

TABLE 1

Minimum inhibitory concentration (MIC, w / v%) S.aureus P.aeruginosa E.coli C.albicans Methylparaben 0.30 0.30 0.35 0.35 Propylparaben 0.15 0.15 0.15 0.10 Phenoxyethanol 0.60 0.50 0.45 0.60 Chlorphenesin 0.30 0.40 0.20 0.45 Caprylyl Glycol, Ethylhexylglycerine Mixed Composition 0.30 0.35 0.30 0.40

As shown in Table 1, the cosmetic composition containing the caprylyl glycol, ethylhexyl glycerine mixed composition was secured similar to or better than conventional preservatives against bacteria and fungi.

Experimental Example 2 Mitigation Effect of Cytotoxicity by Ultraviolet Irradiation

This example was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of juniper extract, leek extract and wild berry extract obtained in Examples 1 to 3.

In this example, juniper extract, leek extract, and wild sesame extract were mixed in a ratio of 1: 1: 1.

Fibroblasts (fibroblasts) were placed in a 24-well test plate 1 x 10 ⁵ and attached for 24 hours. Each well was washed once with PBS and 500 ul of PBS was added to each well. The fibroblasts were irradiated with ultraviolet light 10mJ / cm ² using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. After treatment with the mixed extract (Example 4) to be evaluated therein was incubated for 24 hours. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed and 500 μl of isopropanol-HCl (0.04 N) was added. After shaking for 5 minutes, the cells were lysed and 100 μl of the supernatant was transferred to a 96-well test plate, followed by measurement of 565 nm absorbance in a microplate reader. Cell survival rate (%) was measured by Equation 1, and cytotoxicity relaxation rate by ultraviolet rays was calculated by Equation 2.

Equation 1

Cell survival rate (%) = (St-Bo) / (Bt-Bo) X 100

Bo: 565 nm absorbance of wells that only reacted with cell culture media

Bt: 565 nm absorbance of the wells that developed the colorless reaction wells

St: 565 nm absorbance of the wells that reacted the sample treated wells

Equation 2

Reduction rate of cytotoxicity by UV rays (%) = 1- (St-Bo) / (Bt-Bo) X 100

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: cell viability of wells that were not irradiated with UV light

St: Cell survival rate of wells treated with UV

Experimental results As shown in Table 2, the mixed extract (Example 4) can be seen that the cytotoxicity of the UV by 0.02% at the concentration of 69% to alleviate the cytotoxicity at low concentrations to effectively protect the cytotoxicity at low concentrations .

TABLE 2

Sample Name Treatment concentration (%) Cytotoxic alleviation rate (%) Mixed Extract (Example 4) 0.01 38 0.02 69

Experimental Example 3 Inhibitory Effect of Inflammatory Cytokine Expression by Ultraviolet Irradiation

This example is an inflammatory cytoplasm expressed by ultraviolet irradiation to confirm the anti-inflammatory effect of the juniper extract, leek extract, wild jasmine extract (Example 4) obtained in Examples 1 to 3 It was evaluated the degree of inhibition of the expression of chine.

Fibroblasts isolated from human epidermal tissue (Fibroblast) were placed in a 24-well test plate each 5X10 ⁴ and attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10mJ / cm ² using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki Co., Japan), after which PBS was removed and FBS was not added to the cell culture medium (DMEM). Medium) 350 μL was added. After treatment with the mixed extract (Example 4) to be evaluated therein was incubated for 5 hours. 150 μL of the culture supernatant was taken to quantify IL-1a to determine the inhibitory effect of inflammatory cytokine expression of the mixed extract (Example 4). The amount of IL-1a was quantified using Enzyme-linked Immunosorbent Assay (ELISA), and the production rate of IL-1a was calculated by Equation 3.

Equation 3

Inhibitory rate of inflammatory cytokine expression (%) = 1- (St-Bo) / (Bt-Bo) X 100

Bo: IL-1a production amount in wells that were not irradiated with UV

Bt: IL-1a production amount in wells that were not irradiated with UV light

St: Production of IL-1a in Wells Treated by Ultraviolet Irradiation

Experimental results As shown in Table 6, the mixed extract (Example 4) inhibits the production of inflammatory cytokines IL-1a by UV rays at 73% at 0.02% concentration, effectively preventing the occurrence of inflammation caused by UV rays at low concentrations. It can be seen.

TABLE 3

Sample Name Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Mixed Extract (Example 4) 0.01 42 0.02 73

Experimental Example 4: Skin Primary Irritation Test

In order to determine the skin irritation properties of the products prepared according to the prescription of the antiseptic properties in the cosmetic composition of the preparation example of Table 4, a certain amount of each sample at 50 cm in the lower arm of both arms for 20 healthy adults. ) Was patched for 24 hours, and then removed and the skin condition was visually read after 1 hour and 24 hours, and the results are shown in Table 5.

<Decision Criteria>

-: No erythema or unusual phenomenon

+-: Slightly reddish

+: Significantly redder than the surroundings

++: Severe reddening and swelling around

TABLE 4

Raw material Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Comparative Production Example 1 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 Beeswax 4.0 4.0 4.0 4.0 4.0 4.0 oil 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 Caprylyl Glycol, Ethylhexylglycerine Mixed Composition (Example 5) 0.1 0.2 0.3 0.4 0.4 - Mixed Extract (Example 4) 1.5 1.5 1.5 1.5 - - Methylparaben - - - - - 0.3 Purified water To 100 To 100 To 100 To 100 To 100 To 100

Note) Unit: Weight%

TABLE 5

Cosmetics The number of the subjects Judgment result ++ + +- - Preparation Example 1 20 - - - 20 Preparation Example 2 20 - - - 20 Preparation Example 3 20 - - - 20 Preparation Example 4 20 - - One 19 Preparation Example 5 20 - One 2 17 Comparative Production Example 1 20 - One 4 15

As shown in the skin irritation test results of the above table, caprylyl glycol, ethylhexyl glycerine mixed composition (Example 5) and juniper, leek, a mixture of extracts of wild flowers (Example 4) of the cosmetic composition The degree of irritation was found to be very low compared to the cosmetic preparation of Comparative Preparation Example 1 using a common preservative. In addition, compared to Preparation Example 5 using only caprylyl glycol and ethylhexylglycerin mixed composition, the cosmetic composition of Preparation Example 4, which was mixed with a mixed extract of juniper, leek, and wild sesame extract showed very low irritation. Could. In particular, the cosmetic composition of Preparation Example 3, in which a mixed mixture of caprylyl glycol and ethylhexylglycerine (Example 5) and juniper, leek and wild sesame extract (Example 4) was mixed at 0.3: 1.5% There was no skin irritation at all, and the skin stimulation safety improvement effect was superior to that of the other ingredients.

Experimental Example 5: Stability test of the formulation of a cosmetic containing a mixed composition of caprylyl glycol, ethylhexylglycerin and juniper extract, leek extract, berry extract

The stability according to the temperature change of the cosmetic formulation of the present invention was tested. A mixed extract of caprylyl glycol, ethylhexylglycerin and juniper, leek, and perilla extract (Example 4) Cosmetic preparations of Preparation Examples 1 to 4 containing the extract, methylparaben as an antiseptic without containing all of the above ingredients The cosmetic preparation of Comparative Preparation Example 1 containing 1 was stored in an opaque glass container for 1 week in a constant temperature bath kept constant at 45, and also in an opaque glass container was kept constant at 4 and kept in a completely shaded refrigerator for 1 week. After storage, the degree of discoloration was measured. The results are shown in Table 6 below. At this time, the degree of product discoloration was evaluated by classifying into six grades as follows.

Product discoloration evaluation criteria

0 no change

1 extremely little change

2 little change

3 slightly worse

4 badly changed

5 extremely severe

TABLE 6

Temperature Discoloration degree of test substance Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Comparative Production Example 1 4 ℃ 0 0 0 0 0 45 ℃ 0 0 One 2 0

As can be seen in Table 6, the cosmetics of Preparation Examples 1 to 4 containing caprylyl glycol, ethylhexyl glycerine and a mixed extract of juniper, leek, and perilla extract (Example 4) are discolored. It was found that the stability is excellent as in the cosmetic preparation of Comparative Preparation Example 1 containing methyl paraben as an existing preservative.

Claims (9)

0.1 to 1.0% by weight of caprylyl glycol, 0.1 to 1.0% by weight of echylhexylglycerin and 0.001 to 10.0% by weight of juniper extract, 0.001 to 10.0% by weight of leek extract, 0.001, as a preservative Cosmetic composition containing from 10.0% by weight of the wild sesame extract. The method of claim 1, Caprylyl glycol and ethylhexyl glycerine as the preservative is a cosmetic composition, characterized in that the mixture composition in a ratio of 10: 1 to 1:10. The method of claim 1, The juniper extract is (a) water, (b) anhydrous or hydrous lower alcohol having 1-4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol Cosmetic composition, characterized in that obtained as an extraction solvent. The method of claim 1, The leek extract comprises (a) water, (b) anhydrous or hydrous lower alcohol having 1-4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol Cosmetic composition, characterized in that obtained by the extraction solvent. According to claim 1, The berry extract may comprise (a) water, (b) anhydrous or hydrous lower alcohol having 1-4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol Cosmetic composition, characterized in that obtained as an extraction solvent. The method of claim 1, Juniper extract, leek extract, wild sesame extract is a cosmetic composition, characterized in that the composition is mixed in a ratio of 10: 10: 1 to 1: 1: 10. The method according to any one of claims 1 to 6, Cosmetic composition, characterized in that it is used for skin irritation relief. The method according to any one of claims 1 to 6, Cosmetic composition, characterized in that used for anti-inflammatory. The method according to any one of claims 1 to 6, The cosmetic composition may include a basic cosmetic selected from lotion, emulsion, cream, essence, gel, pack, cleansing cream and foam cleansing; Makeup cosmetics selected from foundation, mascara, eye shadow and eyeliner; Cosmetics for hair of shampoos or rinses; And a formulation selected from the group consisting of body washes for body washes.