KR102360617B1 - Cosmetic composition comprising Commiphora Myrrh and illite as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising, as active ingredients, myrrh and illite having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects by hot water extraction of a mixture obtained by refining and mixing myrrh and illite. will be.

Description

Cosmetic composition comprising Commiphora Myrrh and illite as an active ingredient

The present invention relates to a cosmetic composition comprising myrrh and illite as active ingredients, and more particularly, by hot water extraction of a mixture obtained by refining myrrh and illite, respectively, anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-inflammatory It relates to a cosmetic composition comprising myrrh and illite having a polluting effect as active ingredients.

As we enter the modern age, interest in skin health is increasing. Skin health is directly related to beauty, and accordingly, interest in a method for maintaining skin health is increasing.

Factors that affect skin health include inflammation, bacteria, aging, moisture, UV rays, and air pollutants.

Inflammation of the skin occurs due to various causes. Inflammation is not good for the appearance of the skin even at the time of occurrence, and it is considered as a factor that adversely affects the appearance of the skin as it leaves scars even after the inflammation subsides.

Inflammation is also exacerbated by bacteria that may come into contact with the skin. Bacteria are also considered as one of the factors that adversely affect the skin, and research on methods to prevent the growth of bacteria is ongoing.

Aging is also one of the factors that adversely affect the health of the skin. Although natural aging cannot be prevented, various studies are being conducted on ways to slow it down as much as possible.

The skin needs a certain amount of moisture. Since dry skin cannot have a good effect on skin health, supplying moisture to the skin is also considered an essential element for skin health.

When UV light hits the skin, melanocytes secrete melanin to protect the skin. The dark colored melanin absorbs light and protects cells from UV rays. However, as we enter the modern age, dark skin has an adverse effect on skin beauty, and studies on methods for suppressing the generation of melanin pigment are also being actively conducted.

Meanwhile, the skin comes into contact with various air pollutants. Methods for neutralizing or removing air pollutants themselves are being actively studied.

In order to solve the above problems, studies on cosmetic compositions having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects are being actively conducted. However, in the prior study, since anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects were obtained using chemical components, the problem of causing side effects to the skin, the problem of requiring a lot of manufacturing cost, and ecologically There was a problem that it was difficult to sustain.

Therefore, studies on cosmetic compositions having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects using natural substances are being actively conducted.

Republic of Korea Patent Publication No. 10-1779523 (2017.09.19. Announcement), "Cosmetic composition containing flavonoid derivatives"

In order to solve the above problems, the present invention provides myrrh and illite having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects by hot water extraction of a mixture obtained by refining and mixing myrrh and illite. An object of the present invention is to provide a cosmetic composition comprising as an active ingredient.

In order to solve the above problems, the present invention includes a mixed extract obtained by hot water extraction of a mixture of a myrrh extract extracted from myrrh, pulverized, heated, purified with ethanol and dried, illite, and anti-inflammatory, antibacterial, skin elasticity enhancement , provides a cosmetic composition comprising myrrh and illite as active ingredients, characterized in that it has moisturizing and whitening effects.

In addition, the mixed extract is characterized in that it has an anti-pollution effect.

In addition, the myrrh extract, pulverized, heated, purified with ethanol, and dried illite is characterized in that it is mixed in a weight ratio of 40: 60 to 60: 40.

In addition, it is characterized in that it comprises a supernatant obtained by cooling the mixed extract and separating it by a centrifugal separator.

In addition, the supernatant is characterized in that it contains 0.1 to 10.0% by weight based on the total weight.

The cosmetic composition comprising myrrh and illite as active ingredients according to the present invention has anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects by extracting a mixture obtained by refining and mixing myrrh and illite, respectively, with hot water. .

1 is a graph showing the anti-inflammatory effect of Examples 1 to 5 and Comparative Examples 1 to 3;
2 is a graph showing the antibacterial effect of Examples 1 to 5 and Comparative Examples 1 to 3;
3 is a graph showing the skin elasticity enhancing effect of Examples 1 to 5 and Comparative Examples 1 to 3;
4 is a graph showing the skin moisturizing effect of Examples 1 to 5 and Comparative Examples 1 to 3;
5 is a graph showing the skin whitening effect of Examples 1 to 5 and Comparative Examples 1 to 3;
6 is a graph showing the anti-pollution effect of Examples 1 to 5 and Comparative Examples 1 to 3;

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part “includes” a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, the cosmetic composition comprising myrrh and illite according to the present invention as active ingredients will be described in detail.

The cosmetic composition comprising myrrh and illite as active ingredients according to the present invention is a mixture of myrrh extract extracted from myrrh and illite, which is pulverized, heated, purified with ethanol, and dried with hot water. , characterized in that it has antibacterial, skin elasticity enhancement, moisturizing and whitening effects.

The myrrh ( Commiphora Myrrha ) is a herbal medicine as a rubber resin obtained from myrrh water (Commiphora myrrha Engler, 沒藥樹) or synthetic water (Commiphora molmol Engler, 哈地樹) of the olive tree family. The former is called natural myrrh, and the latter is also called colloidal myrrh. In general, the taste is bitter, slightly astringent, and sticks to the teeth when chewed. Myrrh contains resin, essential oil, and rubber. The myrrh has anti-thrombotic, anti-gastric ulcer, anti-parasitic action, and the like. Also, in ancient Egypt, it was used as a preservative when making mummies.

In the present invention, the cosmetic composition comprising myrrh and illite as active ingredients uses a myrrh extract, which refers to an extract from myrrh.

The myrrh extract may be extracted using a method such as hot water extraction, ultrasonic extraction, steam extraction, compression extraction, and the like. Most suitably, it is preferable to extract using steam extraction. The myrrh extract may be obtained by adding high-temperature steam to myrrh, dissolving the active substance of myrrh in the high-temperature steam, and condensing the steam in which the active substance of the myrrh is dissolved with cooling water.

The myrrh may be fermented. For example, the myrrh is Aspergillus oryzae ( Aspergillus oryzae ), Aspergillus niger niger ), Aspergillus cleaning agent ( Aspergillus sojae ), Trichoderma reesei ) or Rhizopusoryzae ) It may be fermented using one or more strains selected from the group consisting of species. The strain can be purchased commercially as a publicly disclosed strain. When the fermentation process is performed on myrrh, the anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects of the finished composition can be further increased.

The steam extraction may use steam generated by mixing and heating water and ethanol. When steam generated by mixing water and ethanol for steam extraction and heating is used, the anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects of the finished composition can be further increased.

The illite is a kind of clay mineral, and is a product of chemical weathering of silicate minerals on the surface. It was first discovered by Professor Grim in 1937 in the US state of Illinois and named ILLITE. It is distributed in small amounts in Pennsylvania, Quebec, Canada, Australia, and Illinois, and it is known in academia that it has the world's largest reserves in Yeongdong, Chungbuk, Korea. The main components of Illite include SiO ₂ and Al ₂ O ₃ , and it has excellent heavy metal adsorption function, toxic gas adsorption function, far-infrared radiation, and negative ion generation ability. In addition to the name of the illite, the illite has various names such as hydrous mica, finegrained mica, and mica clay mineral. A representative chemical formula is {K _{0.75 [Al 1.75 (} Mg·Fe ²⁺ ) _{0.25 ]} (Si _{3. 50} Al _0.50 ) _{O 10 (OH) 2 } .} Illite has a lower layer charge than muscovite, and the charge is due to a reduction in the isomorphic substitution of Al ^{3 +} and Si ^{4 +} of the tetrahedral plate. A slight isomorphic substitution occurs in the octahedral plate. Illite is non-expandable due to the strong bonding force caused by K ⁺ existing between the layers, and the interlayer spacing is 10A. In X-ray diffraction, it shows a high-order bottom reflection at 1.0 nm, and there are many polytypes of 1M type.

In the present invention, the illite refers to illite that is pulverized, heated, purified with ethanol, and dried.

It is appropriate that the illite is pulverized and made into a powder form. In addition, it is appropriate that the illite is heated to a high temperature. At this time, it will be appropriate that the heating temperature of the illite is 300 ~ 800 ℃.

The pulverized and heated illite can be purified with ethanol and dried. At this time, the illite is mixed with ethanol in a weight ratio of 40: 60 to 60: 40 to purify the illite, and when the ethanol is separated and dried, only the purified illite remains.

Accordingly, the myrrh extract obtained by extracting myrrh, and the illite, which was pulverized, heated, purified with ethanol, and dried are mixed with each other.

At this time, the myrrh extract, pulverized, heated, purified with ethanol, and dried illite will preferably be mixed in a weight ratio of 40: 60 to 60: 40. At this time, if it is out of the mixing ratio, there may be a problem in that the anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects of the finished composition are lowered.

A mixture of the myrrh extract extracted from the myrrh, and the illite, which is pulverized, heated, purified with ethanol, and dried, can be extracted with hot water at a temperature of 100° C. or less.

The mixed extract obtained by extracting the mixture with hot water may be cooled and separated by a centrifugal separator to obtain a supernatant, and it is preferable that the supernatant be contained in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.

In other words, the cosmetic composition comprising myrrh and illite as active ingredients according to the present invention may include a supernatant obtained by cooling the mixed extract and separating it by a centrifugal separator.

In addition, the cosmetic composition comprising myrrh and illite as active ingredients according to the present invention may include 0.1 to 10.0% by weight of the supernatant based on the total weight of the cosmetic composition.

Accordingly, the cosmetic composition comprising the finished myrrh and illite as active ingredients may have anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

Hereinafter, the effect of the cosmetic composition comprising myrrh and illite according to the present invention as an active ingredient will be described in detail through the following Examples, Comparative Examples and Experimental Examples.

Example 1 to 5. myrrh and Illuminate containing as an active ingredient cosmetics Preparation of the composition

A cosmetic composition comprising the myrrh and illite of Examples 1 to 5 as active ingredients was prepared through the following manufacturing process.

S10: Purified water was heated to generate water vapor, the produced water vapor was passed through the myrrh to produce water vapor extracted from the myrrh, and the water vapor extracted from the myrrh was cooled with a cooler to prepare a myrrh extract.

S20: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S30: A mixture was prepared by mixing the myrrh extract of step S10 and the purified illite of step S20 in the weight ratio shown in Table 1 below.

S40: The mixture of step S30 was extracted with hot water at a temperature of 100° C. or less to prepare a mixed extract.

S50: The mixed extract of step S40 was cooled and separated by centrifugation to take only the supernatant and diluted in purified water to 5.0 wt%.

Weight ratio of myrrh extract
(weight%) Weight ratio of refined illite
(weight%) Example 1 30 70 Example 2 40 60 Example 3 50 50 Example 4 60 40 Example 5 70 30

comparative example One. myrrh Preparation of extracts

The myrrh extract of Comparative Example 1 was prepared through the following manufacturing process.

S10: Purified water was heated to generate water vapor, the produced water vapor was passed through the myrrh to produce water vapor extracted from the myrrh, and the water vapor extracted from the myrrh was cooled with a cooler to prepare a myrrh extract.

S10: The myrrh extract of step S10 was separated by a centrifuge, and only the supernatant was taken and diluted in purified water to 5.0% by weight.

comparative example 2. Refined illite Produce

The purified illite of Comparative Example 2 was prepared through the following manufacturing process.

S10: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S20: The mixed extract of step S10 was cooled and separated by centrifugation to take only the supernatant and diluted in purified water to 5.0% by weight.

comparative example 3. myrrh extract and refined illite Preparation of mixtures

A mixture of the myrrh extract and purified illite of Comparative Example 3 was prepared through the following manufacturing process. In Comparative Example 3, unlike Examples 1 to 5, the hot water extraction process was omitted.

S10: Purified water was heated to generate water vapor, the produced water vapor was passed through the myrrh to produce water vapor extracted from the myrrh, and the water vapor extracted from the myrrh was cooled with a cooler to prepare a myrrh extract.

S20: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S30: A mixture was prepared by mixing the myrrh extract of step S10 and the purified illite of step S20 in the weight ratio shown in Table 1 below.

S40: The mixture of step S30 was separated by centrifugation, and only the supernatant was taken and diluted in purified water to 5.0 wt%.

Experimental example 1. Anti-inflammatory effect

In order to confirm the skin trouble alleviation function of the anti-inflammatory effect of the compositions of the present invention prepared in Examples 1 to 5 and Comparative Examples 1 to 3, a PGE2 production inhibitory effect test was performed. (Jeffrey, KH, Anglea, SW, Zoe , S, and Rhia CM, Intracellular Measurement of Prostaglandin E2: Effect of antiinflammatory drugs on cyclooxygenase activity and prostanoid expression, Analytical Biochemistry, 1999, 271, 18-28). After subculture of macrophage-type RAW 264.7 cells several times, 1 X 10 ⁵ each was placed in a 24-well plate and cultured for 24 hours in a 5% CO2 thermostat. After removing the medium, 50 μg of the compositions of Examples 1 to 5 and Comparative Examples 1 to 3 and a new medium containing 450 μg of LPS (1 μg/ml) were simultaneously treated and cultured under the same conditions as the pre-culture. After 24 hours, the culture medium was centrifuged (12,000 rpm, 3 min) to measure PGE2 to obtain a supernatant. The measurement of PGE2 was quantified using Enzyme-Linked Immunosorbent Assay (ELISA) to determine the prostaglandin inhibitory effect of Examples and Comparative Examples, and the results are shown in Table 2.

Prostaglandin inhibition rate (%) Example 1 32.5 Example 2 58.6 Example 3 73.4 Example 4 57.4 Example 5 38.2 Comparative Example 1 12.3 Comparative Example 2 18.2 Comparative Example 3 26.3

As a result, it was confirmed that Example 3, in which the myrrh extract and purified illite were mixed in a 50:50 weight ratio and extracted with hot water, had the highest prostaglandin inhibition rate.

In addition, the compositions of Examples 1 and 5 showed a rather low rate of inhibition of prostaglandin, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate rate of inhibition of prostaglandin. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which only illite was purified had a low prostaglandin inhibition rate. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a low prostaglandin inhibition rate. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and the purified illite and extracting it with hot water.

Experimental example 2. Antibacterial effect

For the antimicrobial activity test of the compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 3, a paper disk agar diffusion method was applied. As a test strain, Escherichia coli was pre-cultured in a liquid medium (beef extract 3.0g, peptone 5.0g, agar 15.0g, distilled water 1.0L, pH 6.8) at 37°C for 8 to 24 hours to activate it. After that, the suspension was adjusted to a constant concentration (Optical Density 0.5 at 620 nm) and diluted, and then inoculated into a Petri dish by 0.1 ml each, and then 10 ml of the sterilized medium was poured to form a medium. Here, 30 μl of the compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 3 were incubated for 24 hours on a sterile paper disk adsorbed thereto, and then the antibacterial activity was measured by the size of the growth inhibition ring around the disk.

Growth inhibition size for E. coli (mm) Example 1 7.2mm Example 2 10.4mm Example 3 12.8mm Example 4 11.9mm Example 5 7.5mm Comparative Example 1 3.3mm Comparative Example 2 3.8mm Comparative Example 3 5.9mm

As a result, it was confirmed that Example 3, in which the myrrh extract and purified illite were mixed at a weight ratio of 50:50 and extracted with hot water, had the widest growth inhibition size against E. coli.

In addition, the compositions of Examples 1 and 5 showed a rather narrow growth inhibition size, but it was confirmed that the compositions of Examples 2 and 4 had a wide growth inhibition size. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which illite was purified only had a narrow growth inhibition size. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a narrow growth inhibition size. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and the purified illite and extracting it with hot water.

Experimental example 3. Increase skin elasticity

In order to examine the effects on the skin of Examples 1 to 5 and Comparative Examples 1 to 3, the effect of promoting collagen biosynthesis was examined by an in vitro test method. The amount of collagen synthesis was measured by PIP (Procollagen Type I C-peptide) EIA assay, and type I precursor collagen C-terminal peptide (PICP), which was synthesized the most, was quantitatively measured.

1×10 ⁴ cells were seeded in a 24-well plate and cultured for 24 hours. After culturing, the medium was replaced with a serum-free medium, and Examples 1 to 5 and Comparative Examples 1 to 3 were added at a concentration of 50 μg/ml, and cultured at 37° C., 5% CO 2 in an incubator for 3 days. As a control, vitamin C, which is known to promote collagen synthesis, was treated at a concentration of 50 μg/ml in each well as a control.

The culture supernatant medium was centrifuged and used in the kit (PIP EIA KIT). A peroxidase-labeled antibody-POD conjugate (MK1O1, TAKARA BIO INC.) was put into a 96-well plate coated with a mouse monoclonal antibody against PIP and reacted, and 20 μl/well of the culture medium was added thereto. added.

After reacting for 3 hours in an incubator at 37 °C and 5% CO2, 100 μl/well of ρ-phenylenediamine, a substrate solution, was added to develop color. After standing at room temperature for 15 minutes, 100 μl/well of the reaction stop solution was added to stop the reaction. Absorbance was measured at 450 nm within 5 minutes, and the amount of collagen was measured by making a graph with the PICP standard concentration, and the results are shown in Table 4 below.

Collagen synthesis (ng/ml) Example 1 267 Example 2 412 Example 3 594 Example 4 423 Example 5 288 Comparative Example 1 116 Comparative Example 2 128 Comparative Example 3 228

As a result, it was confirmed that Example 3, in which the myrrh extract and purified illite were mixed in a weight ratio of 50:50 and extracted with hot water, had the highest collagen synthesis ability.

In addition, the compositions of Examples 1 and 5 showed somewhat low collagen synthesis ability, but it was confirmed that the compositions of Examples 2 and 4 had adequate collagen synthesis ability. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which only illite was purified had a low level of collagen synthesis ability. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a low level of collagen synthesis. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and purified illite and extracting it with hot water.

Experimental example 4. Moisturizing effect

In order to find out the effect of improving skin moisture, an experiment was conducted as follows.

For 25 people in their 20s and 40s without skin disease, each group was divided into 2 groups of 20 people per group, and in each group, Examples 1 to 5 and Comparative Examples 1 to 3 were applied twice daily for 1 month on the face and forearm. allowed to spread Before the start of application, the skin conductivity was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under constant temperature and humidity conditions (24°C, 40% humidity) and taken as the default value, and after 1 week, 2 weeks, and 4 weeks The increase rate was evaluated by measuring the skin conductivity. The results are shown in Table 5 below.

1 week later 2 weeks later after 4 weeks Example 1 32 33 35 Example 2 43 45 48 Example 3 50 54 58 Example 4 42 43 46 Example 5 31 34 36 Comparative Example 1 18 21 22 Comparative Example 2 15 16 17 Comparative Example 3 25 27 28

As a result, in the case of Example 3, the skin conductivity increase rate was very excellent. In general, since skin conductivity is proportional to the amount of skin moisture, it can be confirmed from the above results that the cosmetic composition comprising myrrh and illite of Example 3 as an active ingredient maintains a higher skin moisture content compared to the cosmetic composition that does not.

In addition, the compositions of Examples 1 and 5 showed a rather low increase in skin conductivity, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate increase in skin conductivity. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which only illite was purified had a low increase in skin conductivity. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a low increase in skin conductivity. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and the purified illite and extracting it with hot water.

Experimental example 5. Whitening effect

After putting 5-6 ml of DMEM medium (glucose 4.5 g/L 89%, serum 10%, antibiotic 1%) in a 25 cm 2 T-flask, the B-16 melanoma (ATCC CRL 6323) cell line derived from the cryopreserved mouse was incubated. After inoculation and incubation for 24 hours under 37 ° C. temperature, 5% CO ₂ conditions, cells were separated by treatment with 0.05% trypsin containing 0.02% EDTA, inoculated into a 25cm 2 T-flask, and cultured for 48 hours. . As a result, the number of cells obtained was 5.76 × 10 ⁶ cells per flask. Here, 100 μg/ml of Examples 1 to 5 and Comparative Examples 1 to 3 were diluted in DMEM medium, treated with cultured melanoma cells, and cultured at 37° C. for 5 days. After culture, all the medium was removed, and cells were separated by treatment with 1 ml of saline-phosphate buffer (PBS) containing 0.02% EDTA and 0.05% trypsin, and then centrifuged for 5 minutes to collect only the cells. The collected cells were treated with 5% trichloroacetate (TCA), stirred, and centrifuged to wash the precipitated melanin with a saline-phosphate buffer solution. Then, after dissolving the precipitated melanin by treatment with 1N NaOH, absorbance was measured at 475 nm, and the concentration of melanin was determined from the standard concentration curve of synthetic melanin (Sigma), and the inhibition of melanin production was shown in Table 6 below.

Melanin production inhibition rate (%) Example 1 43.2 Example 2 72.3 Example 3 83.1 Example 4 71.2 Example 5 48.1 Comparative Example 1 27.1 Comparative Example 2 28.3 Comparative Example 3 37.3

As a result, it was confirmed that Example 3, in which the myrrh extract and purified illite were mixed in a 50:50 weight ratio and extracted with hot water, had the most melanin production inhibition rate.

In addition, the compositions of Examples 1 and 5 showed a rather low inhibition of melanogenesis, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate inhibition of melanogenesis. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which only illite was purified had a low level of inhibition of melanin production. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a low level of inhibition of melanin production. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and the purified illite and extracting it with hot water.

Experimental example 6. anti-pollution effect

In general, the degree of cellular damage caused by pollutants is measured using skin keratinocytes that come in direct contact with pollutants, and Examples 1 to 5 and Comparative Examples 1 to 3 are treated by concentration, and the effect of inhibiting skin cell damage is obtained. Confirmed. The experiment was carried out according to a known method.

Human keratinocytes HaCaT at a concentration of 5 X 10 ³ cells/ml were dispensed in a 96-well plate and cultured for 24 hours, followed by benzopyrene (10 μM), ammonium nitrate (500 μg/ml), and formaldehyde (1.5 μg/ml). Each of Examples 1 to 5 and Comparative Examples 1 to 3 were treated, and further cultured for 48 hours. Thereafter, MTT solution of 5 mg/ml concentration was added, incubated for 3 hours, the medium was removed, and 150 μl of dimethyl sulfoxide was added to each well, followed by shaking for 10 minutes, and absorbance was measured at 570 nm. The comparison value after adding the contaminants to before adding the contaminants was expressed as the cell proliferation rate (%).

benzopyrene
(10 μM) ammonium nitrate
(500㎍/ml) formaldehyde
(1.5㎍/ml) Cell proliferation rate (%) Cell proliferation rate (%) Cell proliferation rate (%) Example 1 48 47 49 Example 2 68 65 65 Example 3 74 77 76 Example 4 66 68 69 Example 5 45 46 48 Comparative Example 1 32 31 35 Comparative Example 2 28 26 28 Comparative Example 3 39 42 41

As a result, when Example 3, in which the myrrh extract and purified illite were mixed in a 50:50 weight ratio and hot-water extraction, was applied, it was confirmed that the contaminants had the highest cell proliferation rate.

In addition, the compositions of Examples 1 and 5 showed a rather low cell proliferation rate, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate cell proliferation rate. Therefore, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which myrrh was extracted alone and Comparative Example 2 in which only illite was purified had a low cell proliferation rate. In addition, it was confirmed that Comparative Example 3, in which the myrrh extract and purified illite were simply mixed, also exhibited a low cell proliferation rate. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the myrrh extract and the purified illite and extracting it with hot water.

conclusion.

Through the above Examples, Comparative Examples and Experimental Examples, the cosmetic composition comprising myrrh and illite of the present invention as an active ingredient includes a myrrh extract obtained by extracting myrrh with steam, and illite dried by grinding, heating, and purification with ethanol. It was confirmed that unexpected effects appeared in the process of mixing and hot water extraction of the mixture.

In addition, through the above experimental process, it was confirmed that the appropriate mixing ratio of the myrrh extract and the purified illite was 40:60 to 60:40.

In addition, it was confirmed that it has anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects through the above experimental process.

Accordingly, the present invention specifies that a cosmetic composition comprising myrrh and illite as active ingredients has been developed as a novel composition having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent to that by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (5)

A mixture of myrrh extract extracted from myrrh, pulverized, heated, purified with ethanol, and dried illite in a weight ratio of 40: 60 to 60: 40, hot water extracted at a temperature below 100 ° C. A cosmetic composition comprising myrrh and illite as active ingredients, characterized in that it has anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing and whitening effects, including the separated supernatant. According to claim 1,
The mixed extract is a cosmetic composition comprising myrrh and illite as active ingredients, characterized in that it has an anti-pollution effect. delete delete According to claim 1,
A cosmetic composition comprising myrrh and illite as active ingredients, characterized in that the supernatant is contained in an amount of 0.1 to 10.0% by weight based on the total weight.

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