KR102360618B1 - Cosmetic composition comprising Paeonia suffruticosa ANDR. and illite as an active ingredient - Google Patents

Abstract

The present invention is a cosmetic comprising, as active ingredients, mulberry and illite, which have the effects of reducing pores, inhibiting sebum secretion, enhancing skin elasticity, moisturizing, whitening and anti-pollution, by extracting a mixture obtained by refining and mixing mulberry and illite, respectively, with hot water. to the composition.

Description

A cosmetic composition comprising Mokdanhwa and illite as active ingredients; and a cosmetic composition comprising Paeonia suffruticosa ANDR. and illite as an active ingredient}

The present invention relates to a cosmetic composition comprising mulberry and illite as active ingredients, and more particularly, by extracting a mixture obtained by refining and mixing mulberry and illite, respectively, with hot water, pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, It relates to a cosmetic composition comprising Mokdanhwa and Illite having whitening and anti-pollution effects as active ingredients.

As we enter the modern age, interest in skin health is increasing. Skin health is directly related to beauty, and accordingly, interest in a method for maintaining skin health is increasing.

Factors that affect skin health include inflammation, bacteria, aging, moisture, UV rays, and air pollutants.

Inflammation of the skin occurs due to various causes. Inflammation is not good for the appearance of the skin even at the time of occurrence, and it is considered as a factor that adversely affects the appearance of the skin as it leaves scars even after the inflammation subsides.

Inflammation is also exacerbated by bacteria that may come into contact with the skin. Bacteria are also considered as one of the factors that adversely affect the skin, and research on methods to prevent the growth of bacteria is ongoing.

Aging is also one of the factors that adversely affect the health of the skin. Although natural aging cannot be prevented, various studies are being conducted on ways to slow it down as much as possible, and in particular, studies on ways to enhance skin elasticity are being actively conducted.

The skin needs a certain amount of moisture. Since dry skin cannot have a good effect on skin health, supplying moisture to the skin is also considered an essential element for skin health.

When UV light hits the skin, melanocytes secrete melanin to protect the skin. The dark colored melanin absorbs light and protects cells from UV rays. However, as we enter the modern age, dark skin has an adverse effect on skin beauty, and studies on methods for suppressing the generation of melanin pigment are also being actively conducted.

Meanwhile, the skin comes into contact with various air pollutants. Methods for neutralizing or removing air pollutants themselves are being actively studied.

In order to solve the above problems, research on a cosmetic composition having pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects is being actively conducted. However, in the prior study, because chemical components were used to reduce pores, suppress sebum secretion, enhance skin elasticity, moisturize, whiten and anti-pollution effects, problems that cause side effects on the skin, problems that require a lot of manufacturing cost, There was a problem that it was difficult to sustain ecologically.

Therefore, studies on cosmetic compositions having pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects using natural substances are being actively conducted.

Republic of Korea Patent Publication No. 10-1779523 (2017.09.19. Announcement), "Cosmetic composition containing flavonoid derivatives"

In order to solve the above problems, the present invention provides a mulberry flower having pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects by hot water extraction of a mixture obtained by refining and mixing mulberry flower and illite, respectively. An object of the present invention is to provide a cosmetic composition comprising illite as an active ingredient.

In order to solve the above problems, the present invention includes a mixed extract obtained by hot water extraction of a mixture of a mulberry extract extracted from mulberry and illite, which is pulverized, heated, purified with ethanol and dried, including, It provides a cosmetic composition comprising Mokdanhwa and Illite as active ingredients, characterized in that it has skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

In addition, the mixed extract is characterized in that it has an anti-pollution effect.

In addition, the illite, which is pulverized, heated, purified with ethanol, and dried with the mulberry extract, is characterized in that it is mixed in a weight ratio of 40: 60 to 60: 40.

In addition, it is characterized in that it comprises a supernatant obtained by cooling the mixed extract and separating it by a centrifugal separator.

In addition, the supernatant is characterized in that it contains 0.1 to 10.0% by weight based on the total weight.

The cosmetic composition comprising mulberry and illite as active ingredients according to the present invention reduces pores, suppresses sebum secretion, improves skin elasticity, moisturizes, whitens and anti-pollution by extracting a mixture obtained by refining and mixing mulberry and illite, respectively, with hot water. have an effect

1 is a graph showing the pore-reducing effect of Examples 1 to 5 and Comparative Examples 1 to 3;
2 is a graph showing the skin elasticity enhancing effect of Examples 1 to 5 and Comparative Examples 1 to 3;
3 is a graph showing the moisturizing effect of Examples 1 to 5 and Comparative Examples 1 to 3;
4 is a graph showing the whitening effect of Examples 1 to 5 and Comparative Examples 1 to 3;
5 is a graph showing the anti-pollution effect of Examples 1 to 5 and Comparative Examples 1 to 3;

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, a cosmetic composition comprising mulberry and illite according to the present invention as active ingredients will be described in detail.

The cosmetic composition comprising mulberry flower and illite as active ingredients according to the present invention includes a mixed extract obtained by hot water extraction of a mixture of mulberry extract extracted from mulberry flower, pulverized, heated, purified with ethanol and dried, and dried illite. It is characterized in that it has shrinking, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

The wood grain flower ( Paeonia ) suffruticosa Andr . ) is a plant belonging to the angiospermaceae, also called peony. The flower of the mulberry flower blooms in red-purple in May, has a diameter of 15 cm or more, and has a stalk like a pouch and surrounds the ovary. The number of sepals is 5 and the number of petals is 8 or more. They are not the same size and shape, and have the shape of an upside down egg, irregularly pitted at the edge. The main ingredients of Mokdan include Paeonol, Paeonolide, and Benzoic acid. The main application diseases include various fevers, liver diseases in children, blood circulation disorders, and menstrual irregularities. The root bark of the mokdan is called muldanpi, and the root bark is often used as medicine.

In the present invention, the cosmetic composition comprising mulberry flower and illite as an active ingredient uses a mulberry extract, which refers to an extract from mulberry flower.

The mulberry extract may be extracted using a method such as hot water extraction, ultrasonic extraction, steam extraction, compression extraction, or the like. Most suitably, it is preferable to extract using steam extraction. The wood dandelion extract may be obtained by adding high-temperature steam to mulberry flower, allowing the effective material of mulberry flower to dissolve and seep into the high-temperature vapor, and condensing the water vapor in which the effective substance of mulberry flower is dissolved with cooling water.

Mokdanhwa may have been fermented. For example, the wood grain is Aspergillus oryzae ( Aspergillus oryzae ), Aspergillus niger niger ), Aspergillus cleaning agent ( Aspergillus sojae ), Trichoderma reesei ) or Rhizopusoryzae ) It may be fermented using one or more strains selected from the group consisting of species. The strain may be commercially available as a publicly disclosed strain. When the fermentation process is performed on the mulberry flower, the effects of pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects of the finished composition may be further increased, but this is not essential.

The steam extraction may use steam generated by mixing and heating water and ethanol. When steam generated by mixing water and ethanol for steam extraction and heating is used, the degree of dissolution of the active ingredient of wood dandelion in the generated steam increases. The anti-pollution effect can be further increased. However, this is not essential in the present invention.

The illite is a kind of clay mineral, and is a product of chemical weathering of silicate minerals on the surface. It was first discovered by Professor Grim in 1937 in the US state of Illinois and named ILLITE. It is distributed in small amounts in Pennsylvania, Quebec, Canada, Australia, and Illinois. The main components of Illite include SiO ₂ and Al ₂ O ₃ , and it has excellent heavy metal adsorption function, toxic gas adsorption function, far-infrared radiation, and negative ion generation ability. In addition to the name of the illite, the illite has various names such as hydrous mica, finegrained mica, and mica clay mineral. A representative chemical formula is {K _{0.75 [Al 1.75 (} Mg·Fe ²⁺ ) _{0.25 ]} (Si _{3. 50} Al _0.50 ) _{O 10 (OH) 2 } .} Illite has a lower layer charge than muscovite, and the charge is due to the reduction in isomorphic substitution of Al ^{3 +} and Si ^{4 +} of the tetrahedral plate. A slight isomorphic substitution occurs in the octahedral plate. Illite is non-expandable due to the strong bonding force caused by K ⁺ existing between the layers, and the interlayer spacing is 10A. In X-ray diffraction, it shows a high-order bottom reflection at 1.0 nm, and there are many polytypes of 1M type.

In the present invention, the illite refers to illite that is pulverized, heated, purified with ethanol, and dried.

It is appropriate that the illite is pulverized and made into a powder form. In addition, it is appropriate that the illite is heated to a high temperature. At this time, it will be appropriate that the heating temperature of the illite is 300 ~ 800 ℃.

The pulverized and heated illite can be purified with ethanol and dried. At this time, the illite is mixed with ethanol in a weight ratio of 40: 60 to 60: 40 to purify the illite, and when the ethanol is separated and dried, only the purified illite remains, which can be used for mixing.

Accordingly, the mulberry flower extract obtained by extracting the dandelion flower, and the illite, which is pulverized, heated, purified with ethanol, and dried are mixed with each other.

At this time, it would be preferable to mix the mulberry extract and the illite, which was pulverized, heated, purified with ethanol, and dried in a weight ratio of 40:60 to 60:40. At this time, if it is out of the mixing ratio, there may be problems in that the pore reduction, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects of the finished composition are reduced. For the most appropriate mixing ratio, it would be best to mix the wood dandelion extract and the illite, which has been heated, purified with ethanol, and dried in a weight ratio of 50:50.

A mixture of the mulberry extract extracted from the mulberry flower and the illite dried by grinding, heating, and ethanol purification can be extracted with hot water at a temperature of 100° C. or less.

The mixed extract obtained by extracting the mixture with hot water may be cooled and separated by a centrifugal separator to obtain a supernatant, and it is preferable that the supernatant be contained in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.

In other words, the cosmetic composition comprising Mokdanhwa and illite as active ingredients according to the present invention may include a supernatant obtained by cooling the mixed extract and separating it by a centrifugal separator.

In addition, the cosmetic composition comprising Mokdanhwa and illite as active ingredients according to the present invention may include 0.1 to 10.0% by weight of the supernatant based on the total weight of the cosmetic composition.

Accordingly, the cosmetic composition comprising the finished mulberry flower and illite as active ingredients may have pore-reducing, sebum secretion suppression, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

Hereinafter, the effect of the cosmetic composition comprising mulberry and illite according to the present invention as an active ingredient will be described in detail through the following Examples, Comparative Examples and Experimental Examples.

Example 1 to 5. Woodcut flowers and Illuminate containing as an active ingredient cosmetics Preparation of the composition

Through the following manufacturing process, a cosmetic composition comprising the mulberry flower and illite of Examples 1 to 5 as active ingredients was prepared.

S10: Purified water was heated to generate water vapor, and the produced water vapor was passed through the wood grain flower to produce water vapor extracted from the wood grain flower, and the water vapor extracted from the wood flower flower was cooled with a cooler to prepare a wood grain flower extract.

S20: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S30: A mixture was prepared by mixing the Mokdanhwa extract of step S10 and the purified illite of step S20 in the weight ratio shown in Table 1 below.

S40: The mixture of step S30 was extracted with hot water at a temperature of 100° C. or less to prepare a mixed extract.

S50: The mixed extract of step S40 was cooled and separated by centrifugation to take only the supernatant and diluted in purified water to 5.0 wt%.

Weight ratio of wood dandelion extract
(weight%) Weight ratio of refined illite
(weight%) Example 1 30 70 Example 2 40 60 Example 3 50 50 Example 4 60 40 Example 5 70 30

Comparative Example 1. Preparation of Mokdanhwa extract

Mokdanhwa extract of Comparative Example 1 was prepared through the following manufacturing process.

S10: Purified water was heated to generate water vapor, and the generated water vapor was passed through the wood grain flower to produce water vapor extracted from the wood grain flower, and the water vapor extracted from the wood flower flower was cooled with a cooler to prepare a wood grain flower extract.

S10: The Mokdanhwa extract of step S10 was separated by centrifugation, and only the supernatant was taken and diluted in purified water to 5.0 wt%.

Comparative Example 2. Preparation of purified illite

The purified illite of Comparative Example 2 was prepared through the following manufacturing process.

S10: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S20: The mixed extract of step S10 was cooled and separated by centrifugation to take only the supernatant and diluted in purified water to 5.0% by weight.

Comparative Example 3. Preparation of Mokdanhwa Extract and Purified Illite Mixture

A mixture of the Mokdanhwa extract of Comparative Example 3 and the purified illite was prepared through the following manufacturing process. In Comparative Example 3, unlike Examples 1 to 5, the hot water extraction process was omitted.

S10: Purified water was heated to generate water vapor, and the generated water vapor was passed through the wood grain flower to produce water vapor extracted from the wood grain flower, and the water vapor extracted from the wood flower flower was cooled with a cooler to prepare a wood grain flower extract.

S20: Illite was pulverized, heated to 500° C., mixed with ethanol for purification, and again ethanol was separated and dried to prepare purified Illite.

S30: A mixture was prepared by mixing the Mokdanhwa extract of step S10 and the purified illite of step S20 in the weight ratio shown in Table 1 below.

S40: The mixture of step S30 was separated by centrifugation, and only the supernatant was taken and diluted in purified water to 5.0 wt%.

Experimental example 1. Pore reduction effect

Hemoglobin precipitation rates were measured for Examples 1 to 5 and Comparative Examples 1 to 3. For Examples 1 to 5 and Comparative Examples 1 to 3, the pore contraction effect was confirmed using bovine hemoglobin protein. A mixed solution (1mg/1ml) of PBS (phosphate bufferedsaline, pH 7.4) with hemoglobin was prepared in a 1:1 ratio, centrifuged at 3000 rpm for 5 minutes, and the supernatant was separated and absorbance was measured at 407 nm in an optical instrument. For Examples 1 to 5 and Comparative Examples 1 to 3 contained in the reaction solution, the amount of hemoglobin precipitation was measured to measure the pore-contraction effect, and the results are shown in Table 2. It can be seen that the higher the precipitation rate of hemoglobin, the better the pore-constricting effect.

Hemoglobin precipitation (%) Example 1 54.3 Example 2 78.2 Example 3 84.8 Example 4 73.9 Example 5 52.7 Comparative Example 1 13.4 Comparative Example 2 17.5 Comparative Example 3 23.5

As a result, it was confirmed that Example 3, in which the Mokdanhwa extract and purified illite were mixed in a weight ratio of 50:50 and extracted with hot water, had the highest hemoglobin precipitation rate.

In addition, the compositions of Examples 1 and 5 showed a rather low hemoglobin precipitation rate, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate hemoglobin precipitation rate. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low hemoglobin precipitation rate. In addition, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also exhibited a low hemoglobin precipitation rate. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

Experimental example 2. Sebum secretion inhibitory effect

After gently wiping the foreheads of 20 males aged 34,5 years with 70% ethanol and drying them, divide sebu tape into the left, center, and right sides of the forehead and attach it for 30 minutes. After 30 minutes, remove the sebu tape and attach it to the area where the new sebu tape was removed for 30 minutes and then remove it. After that, 20 μl of the compositions of Examples 1 to 5 and Comparative Examples 1 to 3 were applied to the forehead twice a day for 4 weeks, and the sample was not applied in the morning of the measurement day. On the day of the experiment, attach a new sebu tape in the same way as 4 weeks before, and then remove it.

Sebum measurement for 4 weeks was measured after the test subject was acclimatized to constant temperature and humidity (22℃, 50% humidity) conditions to reduce the influence on the external environment before measurement. The values of the sebu tapes thus obtained were compared through image analysis and shown in Table 3 below.

No improvement/worse
(<10%) slight improvement
(10% ~ 30%) significant improvement
(>30%) Example 1 2 10 8 Example 2 - 5 15 Example 3 - 3 17 Example 4 - 6 14 Example 5 One 11 8 Comparative Example 1 14 4 2 Comparative Example 2 16 3 One Comparative Example 3 13 7 -

As a result, as a result, it was confirmed that Example 3, in which the wood dandelion extract and purified illite were mixed in a weight ratio of 50:50 and extracted with hot water, had the most excellent improvement effect in suppressing sebum secretion.

In addition, the compositions of Examples 1 and 5 showed a rather low improvement effect, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate improvement effect. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low improvement effect. In addition, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also showed a low improvement effect. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

Experimental example 3. Skin elasticity enhancement effect

In order to examine the effects on the skin of Examples 1 to 5 and Comparative Examples 1 to 3, the effect of promoting collagen biosynthesis was examined by an in vitro test method. The amount of collagen synthesis was measured by PIP (Procollagen Type I C-peptide) EIA assay, and the most synthesized type I precursor collagen C-terminal peptide (PICP) was quantitatively measured.

1×10 ⁴ cells were seeded in a 24-well plate and cultured for 24 hours. After incubation, the medium was replaced with a serum-free medium, and Examples 1 to 5 and Comparative Examples 1 to 3 were added at a concentration of 50 μg/ml, and cultured at 37° C., 5% CO 2 in an incubator for 3 days. As a control, vitamin C, which is known to promote collagen synthesis, was treated at a concentration of 50 μg/ml in each well as a control.

The culture supernatant medium was centrifuged and used in the kit (PIP EIA KIT). A peroxidase-labeled antibody-POD conjugate (MK1O1, TAKARA BIO INC.) was put into a 96-well plate coated with a mouse monoclonal antibody against PIP and reacted, and 20 μl/well of the culture medium was added thereto. added.

After reacting for 3 hours in an incubator at 37 °C and 5% CO2, ρ-phenylenediamine, a substrate solution, was added to 100 μl/well to develop color. After standing at room temperature for 15 minutes, 100 μl/well of the reaction stop solution was added to stop the reaction. Absorbance was measured at 450 nm within 5 minutes, and the amount of collagen was measured by making a graph with the PICP standard concentration, and the results are shown in Table 4 below.

Collagen synthesis capacity (ng/ml) Example 1 416 Example 2 534 Example 3 627 Example 4 523 Example 5 456 Comparative Example 1 145 Comparative Example 2 128 Comparative Example 3 334

As a result, it was confirmed that Example 3, in which the Mokdanhwa extract and purified illite were mixed in a weight ratio of 50:50 and extracted with hot water, had the highest collagen synthesis ability.

In addition, the compositions of Examples 1 and 5 showed a rather low collagen synthesis ability, but it was confirmed that the compositions of Examples 2 and 4 had adequate collagen synthesis ability. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low level of collagen synthesis ability. In addition, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also exhibited a low level of collagen synthesis. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

Experimental example 4. Moisturizing effect

In order to find out the effect of improving skin moisture, an experiment was conducted as follows.

For 25 people in their 20s and 40s without skin disease, each group was divided into 2 groups of 20 people per group, and Examples 1 to 5 and Comparative Examples 1 to 3 were applied to the face and forearm for 1 month twice daily for each group. allowed to spread Before the start of application, the skin conductivity was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under constant temperature and humidity conditions (24°C, 40% humidity) and taken as the default value, and after 1 week, 2 weeks, and 4 weeks The increase rate was evaluated by measuring the skin conductivity. The results are shown in Table 5 below.

1 week later 2 weeks later after 4 weeks Example 1 35 37 41 Example 2 53 54 58 Example 3 63 65 68 Example 4 51 52 55 Example 5 36 39 43 Comparative Example 1 20 23 24 Comparative Example 2 15 16 17 Comparative Example 3 28 31 33

As a result, in the case of Example 3, the skin conductivity increase rate was very excellent. In general, since skin conductivity is proportional to the amount of moisture in the skin, it can be confirmed from the above results that the cosmetic composition comprising Mokdanhwa and Illite of Example 3 maintains a high skin moisture content compared to cosmetics that do not.

In addition, the compositions of Examples 1 and 5 showed a rather low increase in skin conductivity, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate increase in skin conductivity. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low increase in skin conductivity. In addition, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also exhibited a low increase in skin conductivity. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

Experimental example 5. Whitening effect

After putting 5-6 ml of DMEM medium (glucose 4.5 g/L 89%, serum 10%, antibiotic 1%) in a 25 cm 2 T-flask, the B-16 melanoma (ATCC CRL 6323) cell line derived from the cryopreserved mouse was incubated. After inoculation and incubation for 24 hours under 37° C. temperature and 5% CO ₂ conditions, cells were isolated by treatment with 0.05% trypsin containing 0.02% EDTA, inoculated in 25 cm 2 T-flask, and cultured for 48 hours. . As a result, the number of cells obtained was 5.76 × 10 ⁶ cells per flask. Here, 100 μg/ml of Examples 1 to 5 and Comparative Examples 1 to 3 were diluted in DMEM medium, treated with cultured melanoma cells, and cultured at 37° C. for 5 days. After culture, all of the medium was removed, and cells were separated by treatment with 1 ml of saline-phosphate buffer (PBS) containing 0.02% EDTA and 0.05% trypsin, and then centrifuged for 5 minutes to collect only the cells. The collected cells were treated with 5% trichloroacetate (TCA), stirred, and centrifuged to wash the precipitated melanin with a saline-phosphate buffer solution. Then, after dissolving the precipitated melanin by treatment with 1N NaOH, absorbance was measured at 475 nm, and the concentration of melanin was determined from the standard concentration curve of synthetic melanin (Sigma), and the inhibition of melanin production was shown in Table 6 below.

Melanin production inhibition rate (%) Example 1 51.3 Example 2 77.6 Example 3 85.9 Example 4 79.1 Example 5 52.3 Comparative Example 1 31.4 Comparative Example 2 28.3 Comparative Example 3 42.6

As a result, it was confirmed that Example 3, in which the Mokdanhwa extract and purified illite were mixed in a 50:50 weight ratio and extracted with hot water, had the most melanin production inhibition rate.

In addition, the compositions of Examples 1 and 5 showed a rather low inhibition of melanogenesis, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate inhibition of melanogenesis. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low level of inhibition of melanin production. And, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also exhibited a low level of inhibition of melanin production. Therefore, it was confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

Experimental example 6. anti-pollution effect

In general, the degree of cell damage caused by pollutants is measured using skin keratinocytes that come in direct contact with pollutants, and Examples 1 to 5 and Comparative Examples 1 to 3 are treated by concentration, and the effect of inhibiting skin cell damage is obtained. Confirmed. The experiment was carried out according to a known method.

Human keratinocytes HaCaT at a concentration of 5 X 10 ³ cells/ml were dispensed in a 96-well plate and cultured for 24 hours, followed by benzopyrene (10 μM), ammonium nitrate (500 μg/ml), and formaldehyde (1.5 μg/ml). Each of Examples 1 to 5 and Comparative Examples 1 to 3 were treated, and further cultured for 48 hours. After that, MTT solution of 5 mg/ml concentration was added and cultured for 3 hours. Then, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570nm. The comparison value after adding the contaminants to before adding the contaminants was expressed as the cell proliferation rate (%).

benzopyrene
(10 μM) ammonium nitrate
(500㎍/ml) formaldehyde
(1.5㎍/ml) Cell proliferation rate (%) Cell proliferation rate (%) Cell proliferation rate (%) Example 1 52 51 55 Example 2 65 64 61 Example 3 82 88 85 Example 4 64 68 68 Example 5 57 58 52 Comparative Example 1 31 33 34 Comparative Example 2 28 26 28 Comparative Example 3 41 42 43

As a result, it was confirmed that the Mokdanhwa extract and purified illite were mixed in a weight ratio of 50:50 and when Example 3, which was extracted with hot water, was applied, the highest cell proliferation rate against contaminants was confirmed.

In addition, the compositions of Examples 1 and 5 showed a rather low cell proliferation rate, but it was confirmed that the compositions of Examples 2 and 4 had an appropriate cell proliferation rate. Therefore, it could be confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

On the other hand, it was confirmed that Comparative Example 1 in which Mokdanhwa was extracted alone and Comparative Example 2 in which Illite was purified only had a low cell proliferation rate. In addition, it was confirmed that Comparative Example 3, in which the Mokdanhwa extract and purified illite were simply mixed, also exhibited a low cell proliferation rate. Therefore, it could be confirmed that an unexpected effect appeared in the process of mixing the mulberry extract and the purified illite and extracting it with hot water.

conclusion.

Through the above Examples, Comparative Examples and Experimental Examples, the cosmetic composition comprising mulberry flower and illite of the present invention as an active ingredient was mixed with mulberry flower extract extracted with steam, pulverized and heated, purified by ethanol, and dried illite. It was confirmed that unexpected effects appeared in the process and hot water extraction process.

In addition, through the above experimental process, it was confirmed that the appropriate mixing ratio of the Mokdanhwa extract and the purified illite was 40:60 to 60:40.

In addition, it was confirmed that it has a pore-reducing effect, a sebum secretion inhibitory effect, a skin elasticity enhancing effect, a skin moisturizing effect, a skin whitening effect, and an anti-pollution effect through the above experimental process.

Accordingly, the present invention specifies that a cosmetic composition comprising Mokdanhwa and Illite as active ingredients has been developed as a novel composition having anti-inflammatory, antibacterial, skin elasticity enhancement, moisturizing, whitening and anti-pollution effects.

Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent to that by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (5)

A mixture obtained by mixing a wood grain flower extract extracted from wood grain flower, pulverized, heated, purified with ethanol, and dried illite in a weight ratio of 40: 60 to 60: 40, hot water extracted at a temperature below 100 ° C. A cosmetic composition comprising Mokdanhwa and Illite as active ingredients, characterized in that it has pore shrinkage, sebum secretion suppression, skin elasticity enhancement, moisturizing and whitening effects, including the separated supernatant. According to claim 1,
The mixed extract is a cosmetic composition comprising Mokdanhwa and Illite as active ingredients, characterized in that it has an anti-pollution effect. delete delete The method of claim 1,
A cosmetic composition comprising Mokdanhwa and Illite as an active ingredient, characterized in that it contains 0.1 to 10.0% by weight of the supernatant based on the total weight.

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