KR102107071B1 - Method for preparing shampoo composition for improving scalp seborrheic dermatitis - Google Patents

Abstract

The present invention relates to a production method of a shampoo composition for alleviating scalp seborrheic dermatitis and, more specifically, to a production method of a shampoo composition for alleviating scalp seborrheic dermatitis, which exhibits high antioxidant and anti-inflammatory effects without causing skin irritation, and effectively alleviates scalp seborrheic dermatitis by removing bacteria which cause scalp seborrheic dermatitis.

Description

METHOD FOR PREPARING SHAMPOO COMPOSITION FOR IMPROVING SCALP SEBORRHEIC DERMATITIS}

The present invention relates to a method for preparing a shampoo composition for improving scalp seborrheic dermatitis, and more specifically, exhibits high antioxidant and anti-inflammatory effects without causing skin irritation, and effectively improves scalp seborrheic dermatitis by removing the cause of scalp seborrheic dermatitis. It relates to a method of manufacturing a shampoo composition for improving scalp seborrheic dermatitis, which can be.

In general, the human hair protects the skull from physical impact from outside, ultraviolet rays, fine dust, and sudden changes in temperature, and is a heavy metal unnecessary for the body such as arsenic (As), mercury (Hg), or zinc (Zn). It serves to absorb and discharge it to the body.

In addition, the human scalp is composed of epidermis, dermis, and subcutaneous tissue, and the outermost epidermis functions to protect sensitive human tissues from being damaged.

The average adult has approximately 100,000 to 150,000 hairs, and each hair undergoes growth and degeneration through a process including anagen, catagen, and telogen. The plunged hair falls on average from 50 to 100 per day and maintains a constant number of hairs.

However, due to unbalanced diet, excessive drinking and smoking, drug use, lack of sleep, childbirth, menopausal disorders, frequent perm and staining, and hormonal imbalance, loss of moisturizing function of the scalp and hair, etc., may cause irritation. In addition, it can further affect hair loss.

Among the diseases that may be caused by the scalp irritation, scalp seborrheic dermatitis is a chronic inflammatory skin disease that occurs in a lot of sebum secretions. The scalp seborrheic dermatitis has erythema and dandruff as the main symptoms, and occurs frequently within 3 months of age or between 40 and 70 years of age, especially in 3 to 5% of adult men.

The seborrheic dermatitis may be caused by over-secretion of the scalp gland, imbalance of hormones, and over-proliferation of scalp cells, and may be caused by over-proliferation, such as mold growing on the skin. In many cases, the recurrence is frequent and itching is accompanied by the patient's scalp wounds and, in severe cases, hair loss.

In particular, dandruff, which is one of the symptoms of seborrheic dermatitis, involves fungi that are resident on the skin, and the causative organisms include the genus Pytrosporom , P.ovale , and Malessezia . , Or Filobasidium species are mainly known.

Due to this trend, external preparations for skin, shampoos, etc. have been developed to remove the causative bacteria and improve the condition of the scalp by washing waste products of the scalp and supplying artificial nutrients.

However, when the ingredients of the above and the improved product remain on the scalp, the scalp may feel irritation such as swelling, itching, or redness, so that consumers with sensitive scalp are reluctant to use the scalp and hair improvement products. Accordingly, there is a need for a scalp-related product that can effectively improve inflammation of the scalp, yet exhibit hypoallergenicity, thereby providing an effect of alleviating and calming the scalp irritation.

Republic of Korea Patent Publication No. 10-2014-0072680

The present invention is to solve the above problems, provides a high oxidative and anti-inflammatory effect without causing skin irritation, and can provide a method for improving the scalp seborrheic dermatitis, which can effectively improve scalp seborrheic dermatitis. I want to.

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object is to prepare a medicinal herb powder by drying and pulverizing Eoseongcho leaves, Donggulle root, and dermis; Extracting an active ingredient by adding a solvent of 10 to 500 times the weight of the herbal medicine powder and heating it first in a temperature range of 60 ° C to 100 ° C; Filtering the solvent from which the active ingredient has been extracted to obtain a filtrate; Separating only the supernatant by precipitating the filtrate; Mixing the supernatant with a liquid composition comprising pyrithione zinc, peppermint oil, preservative, and thickener to form a mixed solution; Extracting the active ingredient by heating the mixed solution to a temperature range higher than the primary heating temperature; Filtering the mixed solution from which the active ingredient is extracted; And concentrating the filtered mixture under reduced pressure to obtain a shampoo composition for improving scalp seborrheic dermatitis, which can be achieved by a method for preparing a shampoo composition for improving scalp seborrheic dermatitis.

Specifically, the solvent is purified water, methanol, ethanol, isopropyl alcohol, n-butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, n-hexane, di Ethyl ether, diclomethane, and combinations thereof.

Specifically, the liquid composition may further include a starch-based gel, a silk peptide, and a kaffir lime leaf extract.

According to the present invention, it is possible to improve the extraction yield of the active ingredient through a two-step complex extraction method, and as a liquid composition, pyrithione zinc, peppermint oil, preservative, thickener, starch-based gel, silk peptide, and kaffir lime Shampoo composition for improving hypoallergenic scalp seborrheic dermatitis, which exhibits high antioxidant and anti-inflammatory effects and effectively improves scalp seborrheic dermatitis by removing the causative agent of scalp seborrheic dermatitis, as it includes all of the leaf extract.

However, the effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a flow chart showing a method for preparing a shampoo composition for improving scalp seborrheic dermatitis according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to examples and drawings of the present invention. These examples are only provided by way of example to illustrate the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

In addition, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs, and in case of conflict, the present specification including definitions. The description will take precedence.

In order to clearly describe the proposed invention in the drawings, parts irrelevant to the description are omitted, and like reference numerals are assigned to similar parts throughout the specification. And, when it is said that a part "includes" a certain component, this means that other components may be further included instead of excluding other components unless otherwise specified. In addition, "part" described in the specification means one unit or block that performs a specific function.

The identification numbers (first, second, etc.) in each step are used for convenience of explanation, and the identification numbers do not describe the order of each step, and each step does not explicitly describe a specific order in the context. It may be carried out differently from the order specified above. That is, each step may be performed in the same order as specified, or may be performed substantially simultaneously, or in the opposite order.

Throughout this specification, the term “extract” or “extract” means preferably extracted by a method selected from the group consisting of hot water extraction, ultrasonic extraction, filtration, reflux extraction, and combinations thereof. It may be, but may not be limited as long as it can extract some or all of the components of the plant.

Hereinafter, embodiments and examples of the present application will be described in detail with reference to the accompanying drawings. However, it may not be limited to these embodiments and examples and drawings herein.

One aspect of the present application, the step of drying and pulverizing Eoseongcho leaves, roots, and dermis to prepare a medicinal herb powder; Extracting an active ingredient by adding a solvent of 10 to 500 times the weight of the herbal medicine powder and heating it first in a temperature range of 60 ° C to 100 ° C; Filtering the solvent from which the active ingredient has been extracted to obtain a filtrate; Separating only the supernatant by precipitating the filtrate; Mixing the supernatant with a liquid composition comprising pyrithione zinc, peppermint oil, preservative, and thickener to form a mixed solution; Extracting the active ingredient by heating the mixed solution to a temperature range higher than the primary heating temperature; Filtering the mixed solution from which the active ingredient is extracted; And concentrating the filtered mixture under reduced pressure to obtain a shampoo composition for improving scalp seborrheic dermatitis.

First, a method of manufacturing a shampoo composition for improving scalp seborrheic dermatitis according to an embodiment of the present application may be described with reference to FIG. 1.

First, to prepare the herbal powder by drying and pulverizing Eoseongcho leaves, Donggul root, and dermis. In one embodiment, the grinding may be used without limitation as long as it can powder the Eoseongcho leaves, round roots, and dermis to a certain size, specifically, ball mills, grinders, mixers, cutters, hammer mills, and their Any one selected from the group consisting of combinations can be used.

Among the above herbal powders, Eoseongcho leaves means leaves of Eoseongcho, a perennial plant of the family Sambaekcho. The Eoseongcho is called meaning that it smells like fishy smell from the leaves and stems, and has various other names such as medicinal wheat, Chinese herbal medicine, juice, and ten.

The Eoseongcho is a variety of herbaceous grasses growing on shady and wet land in the mountains of southern regions including Jeju Island and Ulleungdo Island in Korea, and the leaf shape resembles sweet potato leaves and the stem is red. In the early summer, yellow flowers with four white calyxes bloom one by one in the early summer.

In one embodiment, the Eoseongcho leaves may exhibit a therapeutic effect on inflammatory diseases by including methylnonyl ketone, myrchen, laurinaldehyde, caprinaldehyde, and capric acid. For example, the inflammatory disease may be selected from the group consisting of urethritis, cystitis, uteritis, pneumonia, sinusitis, bronchitis, bleeding, fever, spear, and combinations thereof, but may not be limited thereto.

The Eoseongcho is one of the most potent antibacterial activities among all plants on the earth, and may exhibit dozens of times higher antibacterial activity than one of the antibiotics, sulfamine. Accordingly, the shampoo composition prepared using the leaves of Eoseongcho is selected from the group consisting of E. coli, Mycobacterium, Paratyphoid, gonorrhea, Staphylococcus aureus, filamentous fungus, Ringworm, athlete's foot, and combinations thereof. Bacteria can be bacteriostatic or sterilized.

In one embodiment, the Eoseongcho leaves may exhibit hypertension and detoxification, and may have an effect of lowering the blood sugar level of diabetes.

Among the medicinal herbs powder, donggulle is a perennial plant of the Liliaceae family of Liliaceae, and is widely used as a medicine named Okjuk. The donggulle is mainly distributed in Korea, Japan, and China, and is collected in spring and mainly uses leaves or rhizomes.

In one embodiment, the root of the donggulre includes saponin, convallarin, convallamarin, and vitamin A, and by incorporating the ingredients to help promote metabolism and release toxins Can give. In addition, the roots of the donggulre can exhibit strong antioxidant and anti-inflammatory effects, and accordingly, when used in a cosmetic composition or the like, it can alleviate inflammation occurring in the skin.

In addition, among the herbal medicine powder, the dermis means that the dried fruit peel of the tangerine tree (Citrus aurantium L. subsp. Nobilis Makino), which is an herb, is dried. The dermis tastes bitter and very warm.

In one embodiment, the dermis may act on menopause and parenteral. Specifically, the dermis can promote circulation of the group, eliminate moistening and sputum sputum, and may also exhibit an effect of promoting gastric juice secretion and digestion.

Next, 10 to 500 times the solvent weight of the herbal medicine powder is added to the herbal medicine powder prepared by drying and pulverizing the Eoseongcho leaves, Donggulle root, and dermis, and primary heating is performed to extract the active ingredient.

In one embodiment, the solvent can be used without limitation as long as it can extract the active ingredient of the herbal powder, specifically, purified water, methanol, ethanol, isopropyl alcohol, n-butanol, glycerol, ethylene glycol, propylene Glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, diclomethane, and combinations thereof.

In one embodiment, the primary heating may be performed in a temperature range of about 60 ℃ to about 100 ℃. If extraction is performed at less than about 60 ° C, the pharmacological component contained in the herbal powder may not be dissolved in the solvent, and when it exceeds 100 ° C, other harmful components other than the extracted pharmacological component may be extracted. Primary heating is preferably performed in a temperature range of about 60 ° C to about 100 ° C, specifically about 80 ° C to about 100 ° C.

Next, the solvent from which the active ingredient has been extracted is filtered to obtain a filtrate. In one embodiment, the filtration may be to use one or more filters, for example, a filter of a large size is first filtered using a filter of about 120 mesh, and then about 100 μm or less The cartridge can be used to filter out finely sized impurities and harmful bacteria.

Next, the filtrate filtered through the filter is precipitated at room temperature for about 1 hour to about 10 hours to separate only the supernatant. In the precipitation process, liquid impurities that are not filtered through the filter may sink to the lower layer.

Thereafter, the separated supernatant is mixed with a liquid composition containing zinc pyrithione, peppermint oil, preservative, and thickener to form a mixed solution.

The pyrithione zinc (zinc pyrithione) may act as an antifungal agent by interfering with cell membrane transport in connection with depolarization of the fungal cell membrane, and pyrithrosporium ovalle, which is a causative agent of the seborrheic dermatitis overgrown at the site of seborrheic dermatitis ( Plasmodium) ovale, P.ovale ). In addition, it is possible to reduce the turnover of epidermal keratinocytes, thereby suppressing over-scaling of the epidermis.

In addition, since the pyrithione zinc is not absorbed into the skin, it is safe and does not cause side effects, and can effectively treat dandruff by suppressing abnormal proliferation of scalp cells.

In one embodiment, the pyrithione zinc may be about 0.5 to about 10 parts by weight based on 100 parts by weight of the liquid composition. When the content of the pyrithione zinc is less than about 0.5 parts by weight, the pharmacological effect due to the addition of pyrithione zinc may not be sufficiently exhibited, and when it exceeds about 10 parts by weight, a precipitate may be generated in the shampoo composition to be prepared. The content of zinc pyrithione is preferably about 0.5 to about 10 parts by weight based on 100 parts by weight of the liquid composition.

In one embodiment, the liquid composition may include peppermint oil. The peppermint oil is a pale yellow liquid obtained by distilling the essential oil contained therein by drying the stem and leaves of peppermint, a perennial herbaceous perennial plant, and white as the main component of free menthol generated when cooling the essential oil to 4 ° C or lower. It is also called de-brain oil as it has been removed and refined as the crystalline peppermint brain.

In one embodiment, the peppermint oil contains as an ingredient an alcohol system such as menthol, an ketone system such as menthol, and an oxide system such as 1,8-cineol, and conditioning and refreshing feeling of the skin according to the composition It gives a cool feeling and helps to soothe problem skin by losing and removing the activity of substances harmful to the skin.

In one embodiment, the peppermint oil may be from about 5 to about 20 parts by weight based on 100 parts by weight of the liquid composition. When the content of the peppermint oil is less than about 5 parts by weight, the effect of conditioning and refreshing the shampoo composition to be produced may not appear, and when it exceeds about 20 parts by weight, skin irritation may be caused.

In one embodiment, the liquid composition may include a preservative. The preservative serves to inhibit or reduce the growth of microorganisms when using a shampoo composition in a place with a lot of moisture, such as a bathroom, for example, methyl paraoxybenzoate, ethyl paraoxybenzoate, butyl paraoxybenzoate, methylparaben, Phenoxyethanol, and combinations thereof.

In one embodiment, the preservative may be included in about 0.01 to about 3 parts by weight based on 100 parts by weight of the liquid composition. If the preservative is included in an amount of less than about 0.01 parts by weight, the microbial growth inhibitory effect may not be sufficiently exhibited, and when it exceeds about 3 parts by weight, skin irritation may be caused.

In one embodiment, the liquid composition may include a thickener. The thickener may include, for example, one or more selected from the group consisting of polyquaternium, glucamate, sodium chloride, glycol distearate, guar hydroxy propyltrimonium chloride, and combinations thereof. You can.

In one embodiment, the thickener may be included in about 0.01 to about 3 parts by weight based on 100 parts by weight of the liquid composition. If the thickener is contained in less than about 0.01 parts by weight, the flow chart of the prepared shampoo composition is high, which may cause inconvenience to use, and when it exceeds about 3 parts by weight, skin irritation may be caused.

In one embodiment, the liquid composition may further include a starch-based gel. For example, starch includes glutinous rice, non-glutinous rice, wheat, rice husk, corn, potato, sweet potato, sorghum, tapioca, palm, shellac, amylose starch, and their modified starches, i.e. oxidized starch, acid treated starch, ester starch , Ether starch, crosslinked starch, hydroxyalkyl starch, and combinations thereof.

In one embodiment, the starch-based gel is swelled by heating starch having a high amylose content of the α-helix structure and starch having a high content of amylopectin in a branched structure together with water. It may be formed by. Specifically, when the amylose and amylopectin starch are heated together with water, the amylose and amylopectin form a micelle structure by hydrogen bonding, and when further heated, the micelle structure becomes loose and water penetrates between the loose structures. The viscosity may be increased by swelling, and may have a gel form. Specifically, the glutinous rice starch having a high amylopectin content may be a gel-shaped starch formed by mixing with amylose starch and heating it with water.

In one embodiment, the starch-based gel has an excellent effect of maintaining moisture by exhibiting a gel form, and accordingly, when used in a shampoo composition, may maintain the moisture of the scalp skin at a constant level to alleviate keratin development. In addition, the starch-based gel is a biocompatible material, and has a high vitamin content without preventing irritation of the scalp, thereby preventing aging of the skin.

In one embodiment, the starch-based gel may be included in about 0.05 to about 5 parts by weight based on 100 parts by weight of the liquid composition. If the starch-based gel is contained in an amount of less than about 0.05 parts by weight, the water retention and anti-aging effect may be insignificant, and when it is included in excess of about 5 parts by weight, the viscosity may increase, so the starch-based gel is 100 of the liquid composition. It is preferably included from about 0.05 to about 5 parts by weight based on parts by weight.

In one embodiment, the liquid composition may further include a silk peptide. For example, the silk peptide may include three or more amino acids selected from the group consisting of serine, alanine, tyrosine, glycine, cysteine, threonine, leucine, isoleucine, phenylalanine, arginine, tryptophan, and combinations thereof. have. Specifically, the silk peptide may have a form of alanine-alanine-threonine repeated several to several tens of times.

In one embodiment, the silk peptide may be purchased directly from a place of purchase, manufactured from silk fibroin, or prepared by hydrolysis. For example, the silk peptide may be obtained by removing sericin from a silk protein to prepare silk fibroin, and dissolving the silk fibroin with ethanol and a salt solution to prepare a neutral salt lysate.

In one embodiment, the silk peptide prepared by removing sericin from the silk protein may exhibit an effect of preventing skin aging. Specifically, the silk peptide has a unique amino acid composition in which glycine, alanine, and serine occupy 85% or more of the total protein, and accordingly, has immunomodulatory function, anti-aging action, antibacterial action, antithrombotic action, lipid oxidation inhibitory action, mineral It has physiological activity such as binding activity.

In one embodiment, when the silk peptide is included in an amount of less than about 0.1 parts by weight based on 100 parts by weight of the liquid composition, the above-described pharmacological effect may not be sufficiently exhibited. Since it may increase excessively, it is preferable that the silk peptide is contained in about 0.1 parts by weight or more and less than about 3 parts by weight. Specifically, the silk peptide is about 0.1 to about 3 parts by weight, about 0.1 to about 2 parts by weight, about 0.1 to about 1 part by weight, about 0.5 to about 3 parts by weight, about 0.5 to about 2 parts by weight, about 0.5 To about 1 part by weight, about 1 to about 3 parts by weight, or about 1.5 to about 3 parts by weight.

In one embodiment, the liquid composition may further include a kaffir lime leaf extract. Specifically, the liquid composition may have an effect capable of simultaneously removing pollutants and dead skin cells deposited on the scalp while maintaining the moisturizing and soothing effect of the scalp by further including a kaffir lime leaf extract.

The kaffir lime (kaffir lime) is a type of lime, a tangerine plant, distributed in southern China, Southeast Asia, South Asia and the Pacific. The flavor of the leaves and bark of the kaffir lime is very strong, and especially the kaffir lime leaves are alkaline organic compounds containing nitrogen, alkaloid, limonone, and citronellol having a unique scent. , Nerol, and other organic compounds in high concentrations. Since the kaffir lime leaf extract contains anti-inflammatory and antioxidants, it has excellent efficacy in preventing aging of the scalp skin. In addition, when using the kaffir lime leaf extract topically, it serves to prevent skin infections and bacteria, and has a refreshing scent, which can have a calming effect.

In one embodiment, the kaffir lime leaf extract may be included in about 0.5 to about 5 parts by weight based on 100 parts by weight of the liquid composition. If the kaffir lime leaf extract is contained in less than about 0.5 parts by weight, the antioxidant and anti-inflammatory effects may be insignificant, and when it is included in excess of about 3 parts by weight, the peculiar scent may be noticeable. It is preferably included from about 0.5 to about 3 parts by weight based on 100 parts by weight of the liquid composition. Specifically, it may be included in about 1 to about 2 parts by weight.

Next, the active solution is extracted by heating the mixed solution to a temperature range higher than the primary heating temperature. By making the secondary heating temperature higher than the primary heating temperature, pharmacological components that have not been extracted in the primary heating can be extracted, and other unnecessary components can be discharged as steam to produce a high purity extract. . The secondary heating temperature is about 80 ℃ to about 200 ℃, about 80 ℃ to about 180 ℃, about 80 ℃ to about 150 ℃, about 80 ℃ to about 120 ℃, about 120 ℃ to about 200 ℃, about 120 ℃ to It may be about 150 ℃, or about 150 ℃ to about 200 ℃.

Next, the mixture solution that has been extracted through secondary heating is filtered through a filter to obtain a shampoo composition for improving scalp seborrheic dermatitis. The filtration is for separating the generated impurities after completing the extraction, and may be using one or more filters, for example, filtering a large-size impurity first using a filter of about 120 mesh After that, fine-sized impurities and harmful bacteria can be filtered using a cartridge of about 100 μm or less.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, this example is intended to illustrate the present invention in more detail, and the scope of the present invention is not limited to these examples.

[Examples 1 to 3]

Eoseongcho leaves, donggulle root, and dermis were dried and crushed to a fine size using a ball mill. 100% by weight of purified water was added to the pulverized herbal composition to 100 parts by weight of the herbal composition, followed by primary heating at 85 ° C for 8 hours to extract the active ingredient. After extraction, the filter was double-filtered using a 120 mesh filter and an 80 μm cartridge, and precipitated at room temperature for 8 hours to separate only the supernatant.

The separated supernatant was mixed with the liquid composition of Table 1 below. At this time, the separated supernatant was made equal to the weight of the mixed liquid composition. After mixing with the liquid composition, it was secondarily heated at 145 ° C for 10 hours to extract the active ingredient. After extraction and filtering using a filter and cartridge, it was concentrated under reduced pressure to prepare a shampoo composition for improving scalp seborrheic dermatitis. According to the components of each mixed liquid composition, the prepared shampoo compositions were named as Example 1, Example 2, and Example 3, respectively.

[Comparative example]

Prepared in the same manner as in Examples 1 to 3, but as a liquid composition, pyrithione zinc, peppermint oil, starch-based gel, silk peptide, and kaffir lime leaf extract for mixing to improve the scalp seborrheic dermatitis of the comparative example A shampoo composition was prepared.

Ingredient
(Based on 100 parts by weight of the liquid composition) Example 1 Example 2 Example 3 Comparative example Pyrithione zinc 2.00 2.00 2.00 - Preservative (methyl paraoxybenzoate) 0.30 0.30 0.30 0.30 Disodium cocamido
MFA Sulfosuccinate 1.75 1.75 1.75 1.75 peppermint oil 6.00 6.00 6.00 - Citric acid hydrate 0.50 0.50 0.50 0.50 Purified water Balance Balance Balance Balance Combination spices
(Fancy Jasmine Th-5904190) 0.50 0.50 0.50 0.50 Surfactants
(C ₁₄ -C ₁₆ sodium olefinsulfonate) 15.00 15.00 15.00 15.00 Surfactant (Cocamide D) 25.00 25.00 25.00 25.00 Surfactant (sodium polyoxyethylene lauryl ether sulfate) 18.00 18.00 18.00 18.00 Thickener
(Glycol distearate) 0.20 0.20 0.20 0.20 Thickener (sodium chloride) 0.15 0.15 0.15 0.15 Thickener (polyquaternium-10) 0.15 0.15 0.15 0.15 Tar pigment (Blue No. 1) 0.10 0.10 0.10 0.10 Starch gel
(Amylose starch, glutinous rice starch) 3.00 3.00 3.00 - Silk peptide - 2.00 2.00 - Kaffir Lime Leaf Extract - - 1.50 -

[Experimental Example 1: Inhibitory effect on Pyrrosporum Ovale ( Plasmodium ovale, P.ovale )]

For each scalp seborrheic dermatitis improvement shampoo composition prepared with the composition of Examples 1 to 3, and Comparative Examples, antibacterial activity was tested against Pytrosporrom ovale, a causative agent of scalp seborrheic dermatitis. The antibacterial activity test method for the causative bacteria is as follows.

First, a test fungus solution was prepared by inoculating the causative bacterium Pytrosporum Ovale in BHI broth medium and anaerobic culture. 0.15 ml of the test fungus prepared above was diluted with 15 ml of LB broth medium. Next, the shampoo compositions of Examples 1 to 3 and Comparative Examples were respectively dispensed into 96-well plates, and 200 μm of the prepared dilution solution was added in total to mix. The mixed solution was diluted through a double dilution and redistributed into the 96-well plate.

After incubation for 1 to 2 days at 35 ° C after dispensing, the presence or absence of proliferation of the causative bacteria was determined to the extent of suspension of the solution, and the minimum concentration without proliferation of the bacteria was determined as a MIC (Minimum Inhibitory Concentration) value. If the solution was opaque and it was difficult to determine whether the bacteria were growing, it was confirmed through microscopic observation.

The results are shown in Table 2 below, and the MIC is expressed in terms of the concentration of the active ingredient contained in each composition.

Item pH MIC (ppm) Example 1 5.6 > 62 ppm Example 2 5.6 > 58 ppm Example 3 5.6 > 48 ppm Comparative example 5.6 > 145 ppm

As shown in Table 2, it can be seen that the shampoo composition for improving the scalp seborrheic dermatitis of Examples 1 to 3 showed a lower ppm concentration compared to the shampoo composition for improving the scalp seborrheic dermatitis of Comparative Examples, which was caused by the causative bacteria. It means that it has excellent antibacterial activity.

[Experimental Example 2: Measurement of antioxidant effect]

Samples were prepared by mixing and diluting 10 times distilled water in each of the shampoo compositions for improving scalp seborrheic dermatitis prepared in Examples 1 to 3 and Comparative Examples, and ABTS (2,2-azino) for each sample. -bis-3-ethylbenzo-thiazoline-6-sulfonic acid; Sigma-Aldrich, St. Louis, MO, USA) radical scavenging activity was measured by the method of Pellegri et al. (1998). The measurement method is as follows. ABTS 7.0 mM and potassium persulfate 2.0 mM were mixed at the same ratio and left in the dark for 1 day to form ABTS cations, and then diluted with 1xPBS at an absorbance value of 0.70 ± 0.02 at 730 nm. After adding 10 μL of each sample to 200 μL of the diluted ABTS solution, standing for 60 minutes, absorbance was measured at 730 nm. Table 3 shows the measurement results.

division ABTS radical scavenging activity (%) Example 1 64.35 Example 2 71.54 Example 3 76.85 Comparative example 52.08

As shown in Table 3 above, it was confirmed that the shampoo composition for improving scalp seborrheic dermatitis of Examples 1 to 3 showed more improved ABTS radical scavenging activity compared to the comparative example. In addition, the antioxidant effect is the highest in the shampoo composition of Example 3, which includes all of pyrithione zinc, peppermint oil, preservative, thickener, starch-based gel, silk peptide, and kaffir lime leaf extract as components of the liquid composition. The values are shown.

[Experimental Example 3: Measurement of anti-inflammatory effect]

In order to confirm the effect of relaxing the scalp irritation on each of the shampoo compositions for improving scalp seborrheic dermatitis prepared in Examples 1 to 3 and Comparative Examples, a MEST test was performed using a mouse.

First, 6-week-old BALB / c mice were purchased, and 7 hairs of each abdomen were removed for each treatment group, and 2.5% paraphenylenediamine was treated for 3 days, followed by resting for 1 week. As an irritation indicator, a hair dye was used, and the hair dye was applied to the ears of the mice in the treatment group corresponding to each composition and wiped off after 30 minutes. After one week of rest, the compositions of Examples 1 to 3 and Comparative Examples were applied twice at 1 hour intervals. Thereafter, the hair dye was applied again, and after 24 hours, each of the shampoo compositions of Examples 1 to 3 and Comparative Example was applied twice to each treatment group at 1 hour intervals. After 24 hours, the ear parts of each mouse were pathologically organized by H & E staining, and the weight and thickness were measured, respectively, and the results are shown in Table 4 below.

division Weight average (mg) Thickness average (μm) Example 1 10.25 ± 0.658 266.15 ± 4.287 Example 2 11.58 ± 0.689 255.58 ± 34.582 Example 3 9.98 ± 0.354 228.32 ± 15.586 Comparative example 14.65 ± 0.650 308.35 ± 51.312

As shown in Table 4, the treated group mice of Examples 1 to 3 have significantly reduced the weight and thickness of the ear part compared to the treated group mice coated with the shampoo composition of Comparative Example, in particular, of the liquid composition As a component, it showed the lowest value in the treatment group coated with the shampoo composition of Example 3 containing all of starch-based gel, silk peptide, and kaffir lime leaf extract together with pyrithione zinc, peppermint oil, preservative, and thickener. .

That is, when preparing a shampoo composition using a liquid composition containing all of the herbal extract powder according to the present invention, pyrithione zinc, peppermint oil, preservative, thickener, starch-based gel, silk peptide, and kaffir lime leaf extract, It has been confirmed that synergistic effects can have higher antioxidant, anti-inflammatory, and scalp seborrheic dermatitis-causing bacteria removal effects.

In this specification, only a few examples of the various embodiments performed by the present inventors are described, but the technical spirit of the present invention is not limited to or limited thereto, and can be variously implemented by a person skilled in the art.

Claims (3)

Drying and pulverizing the Eoseongcho leaf, the roots of the root, and the dermis to prepare a medicinal herb powder;
Extracting an active ingredient by adding a solvent of 10 to 500 times the weight of the herbal medicine powder and heating it first in a temperature range of 60 ° C to 100 ° C;
Filtering the solvent from which the active ingredient has been extracted to obtain a filtrate;
Separating only the supernatant by precipitating the filtrate;
Mixing the supernatant with a liquid composition comprising pyrithione zinc, peppermint oil, preservative, and thickener to form a mixed solution;
Extracting the active ingredient by heating the mixed solution to a temperature range higher than the primary heating temperature;
Filtering the mixed solution from which the active ingredient is extracted; And
Concentrating the filtered mixture under reduced pressure to obtain a shampoo composition for improving scalp seborrheic dermatitis
Including,
The liquid composition,
A method of preparing a shampoo composition for improving scalp seborrheic dermatitis, further comprising starch-based gel, silk peptide, and kaffir lime leaf extract.
The method of claim 1, wherein the solvent,
Purified water, methanol, ethanol, isopropyl alcohol, n-butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, diclomethane, And combinations of these, one or more selected from the group consisting of scalp seborrheic dermatitis improvement shampoo composition.
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