KR20160097854A - Anti-wrinkle and anti-skin sagging cosmetic composition containing extracts of hypsizigus marmoreus - Google Patents

Abstract

The present disclosure relates to an anti-wrinkle and anti-skin sagging cosmetic composition prepared by comprising a Hypsizigus marmoreus extract as an active component. An anti-skin sagging cosmetic composition using a Hypsizigus marmoreus extract according to an exemplary embodiment of the present disclosure comprises 0.5 to 10.0 percentages by weight of a Hypsizigus marmoreus extract with a molecular weight of 200,000 to 500,000. Accordingly, an anti-skin sagging cosmetic composition using a Hypsizigus marmoreus extract according to an exemplary embodiment of the present disclosure improves skin wrinkles by moisturizing and antioxidant effects of free and constituent amino acid compounds which are contained in Hypsizigus marmoreus in large amounts. Further, an anti-skin sagging cosmetic composition using a Hypsizigus marmoreus extract according to an exemplary embodiment of the present disclosure gives a lifting effect of pulling sagged skin by enabling crude protein that is various peptide complexes comprising low molecular weight beta-glucan to strengthen immunity and inhibit promotion/decomposition of collagen synthesis internally in the skin.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving wrinkles and skin defects using a mushroom extract of Haemophilus sp.

Disclosure of the present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition for improving wrinkles and skin defects, which is produced by incorporating mushroom extract as an active ingredient.

Herein, the background art relating to the present invention is provided, and they are not necessarily referred to as known arts.

There have been several studies to elucidate the aging mechanism of skin, and as a result, the causes of various skin aging have been revealed.

When the skin is dried by a change in temperature, a decrease in humidity, or wind, the skin physiological activity as a barrier against the outside is lowered and aging is promoted. If exposed to harmful reactive oxygen species or free radicals, oxidative action of the body causes lipid peroxidation, which leads to deformation of various proteins constituting the skin.

Collagen and elastic elastin, which occupy most of the skin dermis (about 70-80% of the dry weight of the skin), are the main proteins produced by fibroblasts. These include the mechanical durability of the skin, , And the like (Journal of American Academy of Dermatology, 21: 610-613 (1989)). Collagen shows a quantitative decrease with increasing age and exposure to ultraviolet light. Collagen is classified according to its morphological and structural characteristics, among which the most well characterized are types I, II, III and IV.

Collagen types I and III constitute the intercellular lipids of the dermis and Collagen type IV is the main constituent of DEJ (Dermal Epidermal Junction). DEJ is located between the epidermis and the dermis of the skin to control the permeation and transport of the material between the two layers. It regulates the differentiation of adjacent skin cells and maintains the firmness of the skin.

Elastin is an elastic fiber with a microfibrils embedded in an amorphous matrix. It has a very low elastic modulus such as rubber, so it can easily be deformed by a small force and easily deformed when it is removed. It has the property of coming back.

Elastin is made up of a very unique amino acid found only in the elastic fibers of lysine, desmosine and isodesmosine, and these amino acids form cross-links in long peptide chains, Rubber-like properties.

On the other hand, skin aging and reduction in elasticity are caused by degradation and destruction of collagen and elastin due to weakening of skin cells (fibroblasts) due to ultraviolet rays and the like. Collagenase and elastase are expressed in the skin such as ultraviolet rays, and this enzyme breaks down normal collagen and elastin in the skin, resulting in skin elasticity deterioration.

The protein fractions obtained from Leguminosae Seeds exhibit an elasticity-enhancing effect (U.S. Patent No. 5,322,839), compositions containing malt extract and the like are useful as collagenase Peptidyl carbamates, peptidyl thiocarbamates, hydroxamates (hydroxamates), hydroxamates (hydroxamates), and the like. ), Cephalosporin, Sulphonate salts and the like (U.S. Patent No. 5,614,489).

Such prior art techniques merely inhibit the activity of collagenase or inhibit the activity of elastase to exhibit skin elasticity increasing effect. These conventional techniques are limited to the dermal layer and thus do not maximize their function, and the effect of immediate lifting (skin elasticity improvement) is minimal.

The present disclosure relates to a method for preventing the aging of skin caused by various oxidative stresses such as active oxygen and the like by maintaining the moisture retention efficiently by using natural materials such as sea grass and mushrooms and also by using various physiologically active substances And to provide a cosmetic composition for improving wrinkles and skin defects by using a mushroom extract of the present invention which exhibits immediate wrinkle improvement and lifting effect by ultimately healing / repairing damaged skin.

In order to solve the above-mentioned problems, the cosmetic composition for improvement of wrinkles and skin defilement using a mushroom extract according to one embodiment of the present invention contains 0.5 to 10.0% by weight of a sea mushroom extract having a molecular weight of 200,000 to 500,000 do.

In the cosmetic composition for improvement of wrinkles and skin deflection improvement using a mushroom extract according to one embodiment of the present disclosure, 7.0% by weight of petroleum jelly, 5.0% by weight of liquid paraffin, 2.0% by weight of wax, 2.0% by weight of polysorbate 60, 2.5 weight percent of sorbitan sesquioleate, 3.0 weight percent squalane, 6.0 weight percent propylene glycol, 4.0 weight percent glycerin, 0.5 weight percent triethanolamine, 0.5 weight percent carboxyvinyl polymer, 0.1 weight percent tocopheryl acetate, By weight and 65% by weight.

A cosmetic composition for improving wrinkles and skin defects using a mushroom extract according to an embodiment of the present disclosure is characterized in that the mushroom extract of the present invention is characterized in that the dried mushroom is dried at a temperature of 30 to 70 DEG C And then submerged for 5 to 20 hours.

In the cosmetic composition for improving wrinkles and skin defilement using a mushroom extract according to an embodiment of the present disclosure, the set polar solvent for extracting is preferably 2 to 10 times the weight of the mushroom .

In the cosmetic composition for improving wrinkles and skin defects using a mushroom extract according to one embodiment of the present disclosure, the polarity extraction solvent may be any one selected from the group consisting of a lower alcohol having 1 to 4 carbon atoms, purified water, or a mixture thereof .

According to the cosmetic composition for improvement of wrinkle and skin sagging using the mushroom extract according to one embodiment of the present disclosure, the moisturizing effect and the antioxidant effect of the glass and the constituent amino acid compound and the phenolic compound, It improves skin wrinkles. Crude protein, which is a complex of various peptide complexes including low-molecular-weight beta-glucan, strengthens the skin intrinsically and inhibits collagen synthesis promotion / decomposition.

Hereinafter, embodiments of a cosmetic composition for improving wrinkles and skin defects using the mushroom extract according to the present disclosure will be described in detail with reference to the accompanying drawings.

It should be understood, however, that the scope of the present disclosure is not limited by the embodiments described below, and that those skilled in the art, having the benefit of the teachings of this disclosure, It will be easily suggested, but this will also be included in the technical idea of the present invention.

It is to be understood that the terms used in the present specification or claims are selected for convenience of explanation and should be interpreted appropriately in accordance with the technical idea of the present disclosure when grasping the technical contents of the present disclosure.

The cosmetic composition for improving wrinkles and skin defects using the mushroom extract according to the present invention contains the mushroom extract as an active ingredient .

In the present disclosure, the sea mushroom extract is contained in an amount of 0.5 to 10.0% by weight based on the total cosmetic composition. More preferably, the sea tangle mushroom extract is contained in an amount of 1.0 to 7.0% by weight.

When the extract is less than 0.5% by weight, the cosmetic composition promotes collagen synthesis, inhibits collagen degradation and aging of the skin are insignificant. When the extract is added in an amount exceeding 10.0% by weight, it causes skin trouble, The synergy effect is small and it is not economical.

In the present disclosure, the sea mushroom extract can be obtained by mixing sea cucumber with 50% by weight of mushroom, 49.2% by weight of solvent (ethanol) and 0.8% by weight of preservative, and extracting with hot water for 5 hours in a 50 ° C water bath.

The cosmetic composition for improving wrinkles and skin defilement using the mushroom extract according to the present invention contains 7.0% by weight of petrolatum, 5.0% by weight of liquid paraffin, 2.0% by weight of wax, 2.0% by weight of polysorbate 60, 2.5 weight percent of squalate, 3.0 weight percent squalane, 6.0 weight percent propylene glycol, 4.0 weight percent glycerin, 0.5 weight percent triethanolamine, 0.5 weight percent carboxyvinyl polymer, 0.1 weight percent tocopheryl acetate, and 55 weight percent to 65 weight percent purified water, .

On the other hand, in the present disclosure, the sea mushroom extract has a molecular weight of 200,000 to 500,000.

According to this, it has good affinity with skin proteins, absorbs other constituents, and tightly condenses the proteins and proteins of the skin to form a thin protein film on the skin.

As a result, it immediately reduces skin elongation. Thus giving a noticeable skin pulling effect.

The mushrooms used in the present invention were moisture content (Moisture) of 7.15 ± 0.55%, Crude ash of 8.37 ± 0.64%, crude protein (Crude protein) of 28.69 ± 2.14%, Crude lipid of 2.74 ± 0.44% Carbohydrate is composed of a composition ratio of 53.04 ± 2.75%.

The mushroom used in the present invention is a mushroom-derived polysaccharide (1-3), (1-4) and (1-6) -β-Glucan) which activates the immune system, a natural moisturizing factor (NMF), which contains 61 kinds of free and structural amino acids.

Here, the free amino acid content is 46.53 mg / g and the constituent amino acid content is 204.87 mg / g.

The cosmetic composition for improving wrinkles and skin defects using the mushroom extract of the present invention can be manufactured into various formulations such as lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray, Eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, pack or the like according to the purpose of use.

Next, in the present disclosure, the sea mushroom extract can be produced as follows.

First, the sea mushrooms are thoroughly washed with water, then dried in the shade.

Next, the dried sea cucumber is extracted by warming the mushroom for the set immersion time at the set immersion temperature using an extraction container.

As the extraction solvent, a polar solvent selected from a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used. Preferably, ethanol or water is added to extract.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

An extraction method such as hot water extraction, reflux cooling extraction, ultrasonic extraction and the like can be used, and it can be extracted one to three times preferably by an extraction method of hot water extraction.

As the extraction solvent, the above-mentioned ones are particularly suitable, but the present invention is not limited thereto, and other mushroom extracts can be prepared using other solvents having physicochemical properties similar to those of the above-mentioned solvents.

The settling temperature is preferably 30 to 70 DEG C, more preferably 40 to 60 DEG C, and most preferably 50 DEG C or so, which is most effective in preventing decay, discoloration and detachment.

The settling time is 5 to 20 hours, preferably 10 hours.

On the other hand, the extracted mushroom extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

The filtration can be performed by removing the solid matter using a filter paper, centrifuging the suspension, and filtering the supernatant under reduced pressure, after obtaining the mushroom extract from the sea tangle.

Concentration can be achieved by concentration of the filtered sea bream mushroom extract in a vacuum rotary condenser at 20 to 100 ° C, preferably 50 to 70 ° C.

On the other hand, in the present disclosure, in addition to the extraction method using the above-described extraction solvent, the sea mushroom extract can be obtained by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (for example, size, charge, Separation by chromatography produced for separation of hydrophilicity and the like, etc.).

Hereinafter, the experimental results on the effect of the mushroom extract on the skin applied to the present disclosure will be described.

Sea mushroom mushroom  Preparation of extract

In the present disclosure, the sea mushroom extract was prepared by crushing 50 g of the sea mushroom into a crusher, immersing it in 200 ml of 100% ethanol, and warming it in a 50 ° C water bath for 5 hours.

Next, the mixture was cooled to room temperature and filtered with a filter paper to obtain a filtrate.

Next, the ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experiment 1: Skin cell proliferation effect

To examine the effect of the mushroom extract on skin cell proliferation applied to the present disclosure, human normal fibroblasts (Korean Cell Line Bank) were inoculated into each well of a 96-well microplate at 1 x 10 ⁴ cells , And cultured in DMEM medium (Sigma) for 24 hours at 37 ° C.

Then, the control group, which was replaced with serum-free DMEM medium containing 50 ~ 500 μg / ml of mushroom extract and a serum-free DMEM medium containing no mushroom extract, was further cultured for 24 hours .

Then, 10 μl of each of the MTT solution (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide: 5 mg / ml) was added and cultured for 4 hours , And the medium was removed. 100 μl of DMSO solution was added to each well, shaken for 20 minutes, and the absorbance of the supernatant was measured at 570 nm using a microplate reader (Molecular Devices, USA).

The cell proliferation effect was calculated by substituting the measured values for "cell proliferation effect (%) = [(absorbance of experimental group - absorbance of control group) / absorbance of control group] × 100", and the results are shown in Table 1 below.

Seaweed extract of mushroom (㎍ / ml) Cell proliferation effect (%) Remarks Control group - 50 18.3 100 38.2 200 70. 5 300 75.9 500 87.2

As shown in Table 1, it can be seen that the test group treated with the extract of Mushroom extract showed a very high fibroblast proliferation effect compared with the control group treated with the mushroom extract.

Experiment 2: Enhancement of collagen synthesis

Human normal fibroblasts (Korean Cell Line Bank) were inoculated into each well of a 96-well microplate at 2 × 10 ⁴ cells / well and cultured in DMEM medium at 37 ° C. for 24 hours.

Then, the control group was replaced with the serum-free DMEM medium supplemented with 50-500 μg / ml of the mushroom extract, and the control group was replaced with the serum-free DMEM medium containing no extract.

After culturing, the supernatant of each well was collected and the amount of newly synthesized collagen was measured by measuring the amount of pro-collagen type IC-peptide (PICP) using a kit (TAKARA, Japan). The amount of PICP was converted into ng / 2 × 10 ⁴ cells, and the results are shown in Table 2 below.

Seaweed extract of mushroom (㎍ / ml) Collagen synthesis amount (ng / 2 × 10 ⁴ ) Remarks Control group 101 50 105 100 131 200 153 300 167 500 199

As shown in Table 2, it can be seen that the synthesis of collagen increases with increasing concentration of mushroom extract, and that the mushroom extract of the sea bream exhibits an excellent collagen synthesis promoting effect.

Experiment 3: Collagenase  Inhibitory effect

The inhibitory effect on the collagenase production of the mushroom extract was measured.

Human fibroblasts were plated at 2 × 10 ⁴ cells / well in a 96-well microtiter plate containing DMEM (Dulbeccomodified Eagle Media) medium containing 2.5% fetal bovine serum, And cultured until it grew to about 80%.

After the treatment, the mushroom extract was treated for 24 hours with concentration, and the cell culture liquid was dumped.

The cell culture broth was measured for collagenase production using a commercially available collagenase measuring device (Amersham Pharmacia, Catalog #: RPN 2610).

First, the cell culture medium, which had been plated on a 96-well plate uniformly coated with primary collagenase antibody, was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours.

After 3 hours, the second collagen antibody conjugated with chromophore was put into a 96-well plate and allowed to react for 15 minutes.

After 15 minutes, the color development induction substance is added and the color is induced at room temperature for 15 minutes. When the color reaction is stopped by adding 1 M sulfuric acid again, the color of the reaction solution is yellow and the degree of yellow is different according to the progress of the reaction.

Absorbance of 96 wells in yellow was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated.

The reaction absorbance of the cell cultures of the cells in which the mushroom extract was not treated was used as a control, and the expression inhibition was evaluated as 100 for the control performance of the control group.

Seaweed extract of mushroom (㎍ / ml) Expression level of collagenase (%) Remarks Control group 100 50 95 100 87 200 80 300 72 500 63

As shown in Table 3, it was confirmed that the inhibitory effect of collagenase expression in cells was increased as the concentration of mushroom extract increased.

Experiment 4: Antioxidant effect

Free Radical Scavenging Activity Test was conducted to examine the antioxidant effect of the mushroom extract applied to the present disclosure.

The free radical scavenging activity test was carried out by modifying the method of KIm et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and a stable free radical DPPH (1,1-diphenyl- picryhydrazyl, Sigma) reagents were used. 150 μl of 0.2 mM DPPH solution (Blank in ethanol) was added to 150 μl of each of the extracts of Haesong mushroom extract at various concentrations. After incubation at room temperature for 30 minutes, an experiment group was set up to measure the absorbance at 517 nm , And the control group was set to use purified water.

After the measurement of the absorbance of the experimental group and the control group, the free radical scavenging effect (%) = 100 - [(absorbance of experiment group - absorbance of blank) / absorbance of control group] × 100 " Respectively.

Seaweed extract of mushroom (㎍ / ml) Free radical scavenging effect (%) Remarks 50 10.1 100 16.9 200 35.2 300 44.3 500 74.4

Referring to Table 4, it can be confirmed that the free radical scavenging ability of the mushroom extract applied to the present disclosure increases as the concentration thereof increases.

Next, experimental results on the effect of the content of mushroom extract on the skin in the cosmetic composition according to the present disclosure will be described.

Preparation of experimental samples

In order to examine the skin wrinkle improving effect and lifting effect (skin elasticity improving effect) of the cosmetic composition containing the sea mushroom extract according to the present disclosure, Comparative Example 1 in which the sea mushroom extract was not included, 0.1 in the sea mushroom extract, 3, 4, 5, 6, and 7, respectively, containing 0.5, 3, 5, 7, 10, and 13% by weight of the mushroom extract, respectively. 5.

Ingredients Content (% by weight) vaseline 7.0 Liquid paraffin 5.0 Wax 2.0 Polysorbate 60 2.0 Sorbitan sesquioleate 2.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Carboxyvinyl polymer 0.5 Tocopheryl acetate 0.1 Preservative / incense Proper amount Purified water To 100

Experiment 5: Skin wrinkle improvement effect

The skin wrinkle improving effect of the cosmetic composition containing the mushroom extract according to the present disclosure was measured in 80 healthy women aged 35 to 50 years. First, the cosmetic composition of each of Examples 1 to 7 and Comparative Example 1 was applied to the face portion for 2 months (morning / evening) for 3 months.

After 3 months, the improvement of wrinkles was evaluated by image analysis of questionnaires and wrinkles.

The subjects' questionnaires were judged to be four stages of no improvement, slight improvement, moderate improvement, and considerable improvement compared to before use. The results are shown in Table 6.

Evaluation sample No improvement Slight improvement Moderate improvement A significant improvement Example 1 6 3 One - Example 2 One 4 4 One Example 3 One 3 4 2 Example 4 0 2 5 3 Example 5 0 3 6 One Example 6 One 3 4 2 Example 7 One 4 3 2 Comparative Example 1 7 3 - -

Referring to Table 6, it can be confirmed that satisfactory results are obtained in Examples 2 to 7, and it can be confirmed that the most satisfactory results are obtained in Examples 4 and 5.

Next, the evaluation by image analysis of wrinkles was carried out by taking a replica under the eyes before the start of the experiment, collecting the replica immediately after the end of the experiment from the same area under the eyes, and performing a two-dimensional analysis The density of the wrinkles was measured. The results of the measurement of the wrinkle density by image analysis are shown in Table 7 as a reduction rate relative to the wrinkle density before use.

Evaluation sample Wrinkle density reduction rate (%) Remarks Example 1 7 Example 2 28 Example 3 30 Example 4 35 Example 5 36 Example 6 35 Example 7 33 Comparative Example 1 5

Referring to Table 7, it can be confirmed that satisfactory results are obtained in Examples 2 to 7 similarly to the questionnaire in Table 6, and it can be confirmed that the most satisfactory results are obtained in Examples 4 and 5 .

Experiment 6: Lifting (skin elasticity improvement) effect

The skin elasticity-enhancing effect of the cosmetic composition containing the mushroom extract according to the present disclosure was measured. 80 healthy women aged 20 years or older (mean age 35 years) were divided into 8 groups (10 persons per group) under the conditions of temperature of 24 to 26 DEG C and humidity of 75%, and the cosmetic compositions of Examples 1 to 7 and Comparative Example 1 The skin elasticity was measured using a skin elasticity tester (Cutometer MPA580, Conrage + Khazaka, Germany) after applying for 2 months (morning / evening) for 3 months. The test result is described as R5 (R5 (12 weeks) -R5 (0 week)) value of Cutometer MPA580. R5 value shows the net elasticity property of the skin. The results are shown in Table 8.

Evaluation sample Skin elasticity improvement effect Remarks Example 1 0.015 Example 2 0.075 Example 3 0.082 Example 4 0.087 Example 5 0.085 Example 6 0.080 Example 7 0.081 Comparative Example 1 0.012

Referring to Table 8, the lifting (skin elasticity improving) effects of Examples 2 to 7 containing the mushroom extract according to the present invention were increased by about 525% to 725% as compared with Comparative Example 1. [ Therefore, it can be confirmed that the cosmetic composition containing the sea mushroom extract effectively improves the lifting effect (skin elasticity improvement).

Claims (5)

A cosmetic composition for improving wrinkles and skin defects by using a mushroom extract containing a mushroom extract having a molecular weight of 200,000 to 500,000 and containing 0.5 to 10.0% by weight of mushroom extract. The method according to claim 1,
7.0 wt% of Vaseline, 5.0 wt% of liquid paraffin, 2.0 wt% of wax, 2.0 wt% of polysorbate 60, 2.5 wt% of sorbitan sesquioleate, 3.0 wt% of squalane, 6.0 wt% of propylene glycol, 4.0 wt% of glycerin, , 0.5% by weight of triethanolamine, 0.5% by weight of carboxyvinyl polymer, 0.1% by weight of tocopheryl acetate and 55% to 65% by weight of purified water. The method according to claim 1,
The above-mentioned mushroom extract,
Wherein the dried sea cucumber is extracted by submerging the dried mushroom in a polarity extraction solvent set at a temperature of 30 to 70 ° C for 5 to 20 hours to improve wrinkles and skin defects. The method of claim 3,
The cosmetic composition for improving wrinkles and skin defects using the extract of Mushroom mushroom according to claim 1, wherein the polar solvent for extracting polar solvent is 2 to 10 times the weight of the mushroom. The method of claim 4,
Wherein the polarity extraction solvent is one selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, purified water, and mixtures thereof.