CN113582996B - Application of hypsizigus marmoreus strain in preparation of folic acid - Google Patents
Application of hypsizigus marmoreus strain in preparation of folic acid Download PDFInfo
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- CN113582996B CN113582996B CN202110615910.0A CN202110615910A CN113582996B CN 113582996 B CN113582996 B CN 113582996B CN 202110615910 A CN202110615910 A CN 202110615910A CN 113582996 B CN113582996 B CN 113582996B
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- folic acid
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims abstract description 94
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229960000304 folic acid Drugs 0.000 title claims abstract description 47
- 235000019152 folic acid Nutrition 0.000 title claims abstract description 47
- 239000011724 folic acid Substances 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims description 5
- 238000004321 preservation Methods 0.000 claims abstract description 23
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 150000007524 organic acids Chemical class 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 14
- 229940088594 vitamin Drugs 0.000 abstract description 9
- 229930003231 vitamin Natural products 0.000 abstract description 9
- 235000013343 vitamin Nutrition 0.000 abstract description 9
- 239000011782 vitamin Substances 0.000 abstract description 9
- 241001534815 Hypsizygus marmoreus Species 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 125000003929 folic acid group Chemical group 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 150000003722 vitamin derivatives Chemical class 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- 229930003451 Vitamin B1 Natural products 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 229930014626 natural product Natural products 0.000 description 3
- 229960003495 thiamine Drugs 0.000 description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 3
- 235000010374 vitamin B1 Nutrition 0.000 description 3
- 239000011691 vitamin B1 Substances 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MSTNYGQPCMXVAQ-KIYNQFGBSA-N 5,6,7,8-tetrahydrofolic acid Chemical compound N1C=2C(=O)NC(N)=NC=2NCC1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-KIYNQFGBSA-N 0.000 description 1
- 206010002065 Anaemia megaloblastic Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000726811 Carpinus betulus Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010061619 Deformity Diseases 0.000 description 1
- 241001534813 Hypsizygus Species 0.000 description 1
- 240000005856 Lyophyllum decastes Species 0.000 description 1
- 235000013194 Lyophyllum decastes Nutrition 0.000 description 1
- 208000000682 Megaloblastic Anemia Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 206010000891 acute myocardial infarction Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000037354 amino acid metabolism Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100001016 megaloblastic anemia Toxicity 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000002686 mushroom body Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D475/00—Heterocyclic compounds containing pteridine ring systems
- C07D475/02—Heterocyclic compounds containing pteridine ring systems with an oxygen atom directly attached in position 4
- C07D475/04—Heterocyclic compounds containing pteridine ring systems with an oxygen atom directly attached in position 4 with a nitrogen atom directly attached in position 2
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses an application of a hypsizigus marmoreus strain in preparing folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCCTCC NO: m2020068, the preservation date is 17 days of 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture Collection. The hypsizigus marmoreus XCXH-2 provided by the invention has the folic acid content obviously higher than that of other varieties, is not influenced by other water-soluble vitamins, has high extraction purity, and can be used as a natural source for folic acid extraction.
Description
Technical Field
The invention belongs to the technical field of edible fungus strains, and particularly relates to application of a hypsizigus marmoreus strain in preparation of folic acid.
Background
Hypsizygus marmoreus (Hypsizygus) is the name Hypsizygus marmoreus, and wild hypsizigus marmoreus is mainly distributed in japan, europe, north america, and the like. Artificial cultivation of hypsizigus marmoreus has been successfully achieved in japan, korea, china, etc., and artificial cultivated hypsizigus marmoreus products have been commercialized. Hypsizygus marmoreus is loved by Asians because of the fat and thick fungus flesh of fruiting bodies, crisp and tender stipe, sweet and delicious taste.
White beech mushroom (white Hypsizygusmarmoreus) is a white strain of hypsizigus marmoreus in the market, and is called as golden branch and leaf in hypsizigus marmoreus, and mushroom bodies are crisp, tender, fresh and smooth, sweet and delicious; hypsizygus marmoreus (Xieweigu) is also known as Hypsizygus marmoreus, hypsizygus marmoreus and Lyophyllum decastes, which is a famous rare hypsizigus marmoreus, is a gray strain of hypsizigus marmoreus, has gray fungus cover and longer fungus handle, has gray upper part of the fungus handle, is introduced from Japan, and has unique crab flavor; the hypsizigus marmoreus (Haixiangu) is a pure white strain of hypsizigus marmoreus, and the stipe is longer than that of hypsizigus marmoreus, and has fine and smooth taste, aromatic smell and delicious taste.
The current strains used by domestic hypsizigus marmoreus manufacturers are basically from Japan, and the currently used dominant strains of hypsizigus marmoreus are numbered wz-29, wz-65 and wz-77; the fruiting of the strain wz-29 is characterized by good quality and relatively low unit yield; the fruiting of wz-65 and wz-77 is relatively high in unit yield, the mushroom cap has a tumor cover, the quality guarantee period is short, and the quality is relatively poor.
Folic acid refers to tetrahydrofolic acid and derivatives thereof, is a water-soluble B-group vitamin, and is an essential component of organisms. Folic acid is involved in nucleotide biosynthesis, amino acid metabolism and methylation circulation. Folic acid and synthetic pathways are critical to cellular metabolism. The deficiency of folic acid causes health problems such as neonatal nerve tube deformity, cancer, cardiovascular disease, megaloblastic anemia, arteriosclerosis, acute myocardial infarction, cerebral apoplexy, etc. The human body does not have the capability of synthesizing folic acid and can only take the folic acid through diet. To meet the needs of normal vital activity, adults should be supplemented with 400 μg of folic acid per day, whereas pregnant women should be supplemented with 600 μg per day.
Recently, with consumer importance on health, preference for substances extracted from natural materials rather than chemical synthesis has increased.
Disclosure of Invention
The invention aims to provide an application of a specific hypsizigus marmoreus strain in preparing folic acid, which can conveniently and rapidly extract folic acid from the hypsizigus marmoreus strain and has high folic acid extraction purity.
In order to achieve the technical purpose, the technical scheme provided by the invention is as follows:
an application of hypsizigus marmoreus strain in preparing folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCCH-2, and the preservation number is CCTCC NO: m2020068, the preservation date is 17 days of 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture Collection.
According to the detection result, the folic acid content of the hypsizigus marmoreus strain per 100g is not lower than 56.8 mug.
In another aspect, the present invention also provides a method for preparing folic acid, comprising the steps of:
(1) Drying and crushing hypsizigus marmoreus, wherein the strain of hypsizigus marmoreus is hypsizigus marmoreus XCCH-2, and the preservation number is CCTCC NO: m2020068, the preservation date is 17 days of 4 months in 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture collection;
(2) Adding water and organic acid into hypsizigus marmoreus, and hydrolyzing under high pressure to obtain extract;
(3) Concentrating and drying the extract.
The natural product source hypsizigus marmoreus XCXH-2 selected by the invention has higher folic acid content, and other vitamins, especially water-soluble vitamin C and vitamin B1, have extremely low content, so that the hypsizigus marmoreus can be extracted by a simpler extraction process, and the extraction purity is high.
As a further improvement of the above technical scheme, wherein the organic acid in step (2) is formic acid or acetic acid. Formic acid or acetic acid can stabilize folic acid structure during extraction, prevent folic acid structure from being damaged by heating, and improve folic acid extraction yield.
As a further improvement of the technical scheme, the weight ratio of the hypsizigus marmoreus to the water in the step (2) is 1:10-20. If the amount of water used is too small, folic acid cannot be sufficiently extracted; if too much water is used, folic acid can be diluted in water, the concentration is low, the subsequent concentration and drying needs longer time, and the production efficiency is reduced.
As a further improvement of the technical scheme, the weight ratio of the water to the organic acid in the step (2) is 100:1-5. If the organic acid content is too low, the organic acid does not play a sufficient role; if the organic acid content is too high, the acidity of the whole extract will become strong and folic acid may be destroyed.
As a further improvement of the technical scheme, the heating pressure in the step (2) is 0.1-0.12 Mpa, the heating temperature is 80-100 ℃, and the heating time is 2-5 hours. If the heating temperature is lower than 80 ℃, folic acid is not fully extracted; if the heating temperature exceeds 90℃or the heating time is too long, the structure of folic acid may be destroyed.
Through detection and research on specific hypsizigus marmoreus XCXH-2, the invention discovers that the content of folic acid in the hypsizigus marmoreus is high, the content of other water-soluble vitamins is low, and when the hypsizigus marmoreus is applied to folic acid extraction, the extraction process is simple and the purity is high.
Drawings
FIG. 1 is a photograph of hypsizigus marmoreus XCXH-2 during harvest.
Detailed Description
The following describes the invention in further detail with reference to specific examples. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and biological materials, unless otherwise specified, are commercially available.
Example 1: hypsizygus marmoreus XCXH-2 folic acid and other vitamin content detection
Collecting Hypsizigus marmoreus XCXH-2 (shown in figure 1) in harvesting period, and delivering to Hua-Ji detection authentication group stock limited company for folic acid and other vitamin content detection. The detection methods of all substances are carried out according to detection standards regulated in the industry, and the detection results are shown in the following table 1.
Table 1: hypsizygus marmoreus XCXH-2 folic acid and other vitamin contents
As can be seen from the detection results, the hypsizigus marmoreus XCXH-2 has higher content of leaf acid, but other vitamins, especially water-soluble vitamin C and vitamin B1, have extremely low content, and are very suitable for being used as a natural product source for extracting folic acid.
Example 2: extraction of hypsizigus marmoreus XCXH-2 folic acid
(1) Weighing dried hypsizigus marmoreus XCCH-2 (100 g) and chopping, wherein the preservation number of the hypsizigus marmoreus XCCTCC NO: m2020068, the preservation date is 17 days of 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture Collection.
(2) 1500ml of distilled water is added into hypsizigus marmoreus, and the hypsizigus marmoreus XCXH-2 is immersed. If the amount of water used is too small, folic acid cannot be sufficiently extracted; if too much water is used, folic acid can be diluted in water, the concentration is low, the subsequent concentration and drying needs longer time, and the production efficiency is reduced.
(3) Continuously adding 7.5ml of acetic acid, and uniformly mixing. Acetic acid can stabilize folic acid structure during extraction, prevent folic acid structure from being damaged by heating, and improve folic acid extraction yield. If the organic acid content is too low, the organic acid does not play a sufficient role; if the organic acid content is too high, the acidity of the whole extract will become strong and folic acid may be destroyed.
(4) The mixture was heated to 80℃under 0.1MPa for 2 hours. If the heating temperature is lower than 80 ℃, folic acid is not fully extracted; if the heating temperature exceeds 90℃or the heating time is too long, the structure of folic acid may be destroyed.
(5) Concentrating and drying the extract. The concentration and drying may be performed by vacuum concentration, freeze-drying concentration, or the like, which are commonly used in the art.
The natural product source hypsizigus marmoreus XCXH-2 selected by the invention has higher folic acid content, and other vitamins, especially water-soluble vitamin C and vitamin B1, have extremely low content, so that the hypsizigus marmoreus can be extracted by a simpler extraction process, and the extraction purity is high.
The embodiments of the present invention have been described in detail above in connection with experimental data, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, and yet fall within the scope of the invention.
Claims (7)
1. An application of hypsizigus marmoreus strain in preparing folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCCH-2, and the preservation number is CCTCC NO: m2020068, the preservation date is 17 days of 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture Collection.
2. The use as claimed in claim 1, wherein the folic acid content per 100g hypsizigus marmoreus strain is not lower than 56.8 μg.
3. A method of preparing folic acid comprising the steps of:
(1) Drying and crushing hypsizigus marmoreus, wherein the strain of hypsizigus marmoreus is hypsizigus marmoreus XCCH-2, and the preservation number is CCTCC NO: m2020068, the preservation date is 17 days of 4 months in 2020, the preservation address is Wuhan mountain of Hubei province, and the preservation unit is China center for type culture collection;
(2) Adding water and organic acid into hypsizigus marmoreus, and hydrolyzing under high pressure to obtain extract;
(3) Concentrating and drying the extract.
4. A process for the preparation of folic acid as claimed in claim 3, wherein the organic acid in step (2) is formic acid or acetic acid.
5. The method for preparing folic acid as claimed in claim 4, wherein the weight ratio of hypsizigus marmoreus to water in the step (2) is 1:10-20.
6. The process for preparing folic acid as claimed in claim 5, wherein the weight ratio of water to organic acid in the step (2) is 100:1-5.
7. A process for the preparation of folic acid as claimed in claim 3, wherein in step (2) the heating pressure is between 0.1 and 0.12MPa and the heating temperature is between 80 and 100 ℃.
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Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060018867A1 (en) * | 2004-05-12 | 2006-01-26 | Ichimaru Pharcos Co., Ltd | Cosmetic composition and production thereof |
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