CN107412281A - A kind of preparation method of russule extract - Google Patents

A kind of preparation method of russule extract Download PDF

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Publication number
CN107412281A
CN107412281A CN201710294701.4A CN201710294701A CN107412281A CN 107412281 A CN107412281 A CN 107412281A CN 201710294701 A CN201710294701 A CN 201710294701A CN 107412281 A CN107412281 A CN 107412281A
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Prior art keywords
russule
preparation
extract
added
weight
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CN201710294701.4A
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Chinese (zh)
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佘延英
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佘延英
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Priority to CN201710294701.4A priority Critical patent/CN107412281A/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/07Basidiomycota, e.g. Cryptococcus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material
    • A61K2236/19Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/51Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Abstract

The invention belongs to plant extracts preparing technical field, more particularly to a kind of preparation method of russule extract, including following preparation process:1) fresh russule is plucked, is dried, is crushed;2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.001~0.006% is added and is fermented;3) water, ultrasonic wave extraction are added;4) concentrate, dry.Russule extract is prepared using the inventive method and is advantageous to the preservation of russule, and effectively improves the anticancer function of russule, is improved its value, is increased economic efficiency.

Description

A kind of preparation method of russule extract
Technical field
The invention belongs to plant extracts preparing technical field, more particularly to a kind of preparation method of russule extract.
Background technology
Russule, also known as russule, Classification system:Russula vinosa Lindblad, formed in the very long years Red vertebra woods soil humic layer under hot and humid specific weather condition, the natural edible bacterium that grows naturally.It is a kind of big Type mycorrhizal fungi, belong to Basidiomycotina, Hymenomycetes, Agaricales, Russulaceae, Liaoning, Jiangsu, river are distributed mainly in China The ground such as west, Fujian, Guangxi, Sichuan, Yunnan, wherein the most famous with the russule of Putian, fujian Province, the western band in Fujian.Russule is as wild Rare precious mushroom product, there is very high edibility and medical value.Russule body contains 5 kinds of polysaccharide, 16 kinds of amino acid and 28 Kind aliphatic acid.Polyoses content is about 33.9% that 2.47%, wherein monose and oligosaccharides account for total reducing sugar, amino acid content 14.7%, its In needed by human, semi-dispensable amino acid account for the 54.4% of amino acid problem.Russule dry product contains 15.7 grams of protein, carbon water per hectogram 63.3 grams of compound, 23 milligrams of calcium, 500 milligrams of phosphorus, 23.54 milligrams of vitamin B, 42.3 milligrams of niacin.Russule is not only to seek The very high food of foster value, but also be the health products with drug effects such as enriching yin, kidney tonifying, moistening lung, promoting blood circulation, brain tonic, beauty treatments, often It is edible, energy physical fitness, promote longevity.In addition, modern pharmacological research finds that russule has anticancer, hypoglycemic, reducing blood lipid, raising Oxidative damage caused by immunity of organisms, antibacterial, removing free radical and reparation formaldehyde and other effects.
But russule is not easy to maintain, drying and processing must be carried out after harvesting at once, and is inhaled even if can also exist after drying Tide water suction, makes russule Quality Down, causes economic loss.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preparation method of russule extract, and this method can have Effect preserves russule, and solution russule, which is difficult to ensure, sends one's regards to topic.
To achieve the above object, the present invention adopts the following technical scheme that:
The present invention provides a kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, is dried, is crushed;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.001~0.006% is added and is sent out Ferment;
3) water, ultrasonic wave extraction are added;
4) concentrate, dry.
Preferably, in step 1) of the present invention, 130~160 mesh are crushed to.The russule powder diameter of the present invention can not Too greatly can not be too small, it otherwise can influence extraction effect below.
Preferably, in step 2) of the present invention, fermented 12~18 hours at 28~34 DEG C.By after fermentation process again Ultrasonic wave extraction is carried out, is advantageous to improve the anticancer function of russule.
Preferably, in step 3) of the present invention, the weight of added water is 8~15 times of russule weight.
Preferably, in step 3) of the present invention, 8~16min of ultrasonic wave extraction under 350~600W.
Preferably, temperature during ultrasonic wave extraction of the present invention is 38~46 DEG C.
Preferably, in step 4) of the present invention, relative density is concentrated into as 1.1~1.2.
Preferably, in step 4) of the present invention, drying mode is spray drying.
In the method for the invention, selected must be the fresh russule just plucked, and otherwise can reduce extraction Quality, requirement is not reached.
Russule extract is prepared using the inventive method and is advantageous to the preservation of russule, and effectively improves the anti-of russule Cancer effect, its value is improved, is increased economic efficiency.
Embodiment
The invention will be further described with reference to embodiments, but the invention is not limited in these embodiments.
Embodiment 1
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 130 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.006% is added, ferments 18 at 28 DEG C Hour;
3) 8 times of water of russule weight, the ultrasonic wave extraction 16min at 600W and 38 DEG C are added;
4) it is 1.1 to be concentrated into relative density, spray drying.
Embodiment 2
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 140 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.005% is added, ferments 17 at 29 DEG C Hour;
3) 9 times of water of russule weight, the ultrasonic wave extraction 15min at 550W and 40 DEG C are added;
4) it is 1.12 to be concentrated into relative density, spray drying.
Embodiment 3
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 150 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.004% is added, ferments 15 at 30 DEG C Hour;
3) 10 times of water of russule weight, the ultrasonic wave extraction 14min at 500W and 42 DEG C are added;
4) it is 1.14 to be concentrated into relative density, spray drying.
Embodiment 4
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 160 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.003% is added, ferments 14 at 31 DEG C Hour;
3) 12 times of water of russule weight, the ultrasonic wave extraction 12min at 450W and 44 DEG C are added;
4) it is 1.16 to be concentrated into relative density, spray drying.
Embodiment 5
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 130 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.002% is added, ferments 13 at 32 DEG C Hour;
3) 14 times of water of russule weight, the ultrasonic wave extraction 10min at 400W and 45 DEG C are added;
4) it is 1.18 to be concentrated into relative density, spray drying.
Embodiment 6
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 160 mesh;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.001% is added, ferments 12 at 34 DEG C Hour;
3) 15 times of water of russule weight, the ultrasonic wave extraction 8min at 350W and 46 DEG C are added;
4) it is 1.2 to be concentrated into relative density, spray drying.
Embodiment 7
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 150 mesh;
Remaining step is the same as embodiment 4.
Embodiment 8
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 140 mesh;
Remaining step is the same as embodiment 4.
Embodiment 9
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 130 mesh;
Remaining step is the same as embodiment 4.
Comparative example 1
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 170 mesh;
Remaining step is the same as embodiment 4.
Comparative example 2
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 180 mesh;
Remaining step is the same as embodiment 4.
Comparative example 3
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 120 mesh;
Remaining step is the same as embodiment 4.
Comparative example 4
A kind of preparation method of russule extract, including following preparation process:
1) fresh russule is plucked, drying, is crushed to 110 mesh;
Remaining step is the same as embodiment 4.
Comparative example 5
A kind of preparation method of russule extract, including following preparation process:
Fresh russule is taken, adds 13 times of water of russule weight, 30min is decocted, filtering, obtains russule extraction Liquid.
Test example:The russule extract antineoplastic action effect of the present invention
L materials and methods
1.1 sample
Embodiment 4 and the russule extract of embodiment 6~9, and the russule extract of comparative example 1~4.
Test dose is 4mg/kg.
1.2 tumor-bearing mice
There is provided by Guangxi Medical University's Experimental Animal Center.
1.3 experimental animal
Male ICR mouse 110,22~24g of body weight, is provided by Guangxi Medical University's Experimental Animal Center.
Animal is randomly divided into Normal group (giving 0.5% starch fluid), embodiment 4 and the extraction of the russule of embodiment 6~9 Thing, and the russule extract of comparative example 1~4 are configured to suspension with 0.5% starch fluid (boiling, after cooling).All examinations Test animal and give edible full-valence pellet feed, drinking-water of freely ingesting.
1.4 test method
The gavage amount that each group animal gives tested material is 4mg/kg, l times/d, subcutaneous per mouse right fore when gavage is to 20d Aseptic inoculation S-l80 sarcoma cells suspension [25% (volume fraction), with normal saline dilution] 0.2mL.Continue gavage after inoculation Stop gavage after l0d, after 3d, cervical dislocation puts to death mouse, and tumour is peeled off per mouse, and tumor surface blood stains claim after being blotted with filter paper Weight.
1.5 data statistics
The data obtained each experimental group is compared.
2 result of the tests
2.1 body weight
Each group mouse weight situation of change is shown in Table l.No significant difference (P>0.05).
The each group russule extract of table 1 suppresses Tumor Assays mouse weight situation means standard deviation
Group Number of animals (only) Starting weight (g) 20d body weight (g) 33d body weight (g)
Normal group 10 22.5±0.2 34.2±1.8 31.6±2.1
Embodiment 4 10 23.6±0.4 35.3±2.2 30.2±2.1
Embodiment 6 10 24.2±0.4 34.7±1.5 34.5±1.5
Embodiment 7 10 23.1±0.2 35.4±1.8 32.5±1.9
Embodiment 8 10 22.8±0.3 35.6±1.6 31.4±1.8
Embodiment 9 10 22.3±0.1 34.8±1.9 31.8±2.4
Comparative example 1 10 24.1±0.2 34.9±1.9 30.8±2.2
Comparative example 2 10 22.5±0.3 34.3±2.1 30.4±1.8
Comparative example 3 10 21.9±0.2 33.9±2.1 31.4±1.4
Comparative example 4 10 23.4±0.1 35.8±2.2 31.1±2.0
Comparative example 5 10 24.1±0.2 32.7±1.7 32.4±2.1
Note:Body weight is to go body weight after knurl during 33d.
2.2 suppress the experiment of S-180 sarcomas
Suppress S-l80 sarcomas experiment each group mouse knurl weight and the results are shown in Table 2.Experimental data therein, it is mean value ± standard Difference.
Influence of each group russule extract of table 2 to small s-180 sarcoma weight
Group Number of animals (only) Knurl weight (g)
Normal group 10 1.76±0.12
Embodiment 4 10 0.72±0.19*
Embodiment 6 10 0.76±0.25*
Embodiment 7 10 0.75±0.19*
Embodiment 8 10 0.70±0.19*
Embodiment 9 10 0.83±0.19*
Comparative example 1 10 1.30±0.19*
Comparative example 2 10 1.28±0.19*
Comparative example 3 10 1.26±0.19*
Comparative example 4 10 1.22±0.19*
Comparative example 5 10 1.36±0.19*
Note:* Dunnett'sT is examined, and relatively has conspicuousness, P with Normal group<0.05.
The result of table 2 shows, the anticancer function of russule is effectively improved using the inventive method preparation russule extract, and Powder particle diameter scope is determined, the more effective anticancer function for improving russule.

Claims (8)

1. a kind of preparation method of russule extract, it is characterised in that including following preparation process:
1) fresh russule is plucked, is dried, is crushed;
2) water of russule equivalent weight is added, the saccharomycete of russule weight 0.001~0.006% is added and is fermented;
3) water, ultrasonic wave extraction are added;
4) concentrate, dry.
2. the preparation method of russule extract according to claim 1, it is characterised in that:In the step 1), crush To 130~160 mesh.
3. the preparation method of russule extract according to claim 1, it is characterised in that:In the step 2), 28 Fermented 12~18 hours at~34 DEG C.
4. the preparation method of russule extract according to claim 1, it is characterised in that:It is added in the step 3) The weight of water is 8~15 times of russule weight.
5. the preparation method of russule extract according to claim 1, it is characterised in that:In the step 3), 350 8~16min of ultrasonic wave extraction under~600W.
6. the preparation method of russule extract according to claim 5, it is characterised in that:Temperature during ultrasonic wave extraction For 38~46 DEG C.
7. the preparation method of russule extract according to claim 1, it is characterised in that:In the step 4), concentration It is 1.1~1.2 to relative density.
8. the preparation method of russule extract according to claim 1, it is characterised in that:In the step 4), dry Mode is spray drying.
CN201710294701.4A 2017-04-28 2017-04-28 A kind of preparation method of russule extract Pending CN107412281A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108410656A (en) * 2018-06-07 2018-08-17 张承云 The preparation process of cancer-resisting Russule spirit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102273637A (en) * 2011-08-30 2011-12-14 广东医学院 Application of monascus mycelium extracts to preparation of health-care food and medicines for preventing osteoporosis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102273637A (en) * 2011-08-30 2011-12-14 广东医学院 Application of monascus mycelium extracts to preparation of health-care food and medicines for preventing osteoporosis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
梁文汇,等: "红椎菌营养粉的制作工艺", 《农业研究与应用》 *
高文远: "《中药生物工程》", 31 January 2014, 上海科学技术出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108410656A (en) * 2018-06-07 2018-08-17 张承云 The preparation process of cancer-resisting Russule spirit

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