CN113582996A - Application of hypsizigus marmoreus strain in preparation of folic acid - Google Patents
Application of hypsizigus marmoreus strain in preparation of folic acid Download PDFInfo
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- CN113582996A CN113582996A CN202110615910.0A CN202110615910A CN113582996A CN 113582996 A CN113582996 A CN 113582996A CN 202110615910 A CN202110615910 A CN 202110615910A CN 113582996 A CN113582996 A CN 113582996A
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- folic acid
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims abstract description 96
- 235000019152 folic acid Nutrition 0.000 title claims abstract description 49
- 239000011724 folic acid Substances 0.000 title claims abstract description 49
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229960000304 folic acid Drugs 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title abstract description 4
- 238000004321 preservation Methods 0.000 claims abstract description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 150000007524 organic acids Chemical class 0.000 claims description 12
- 241001534815 Hypsizygus marmoreus Species 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 14
- 229940088594 vitamin Drugs 0.000 abstract description 8
- 229930003231 vitamin Natural products 0.000 abstract description 8
- 235000013343 vitamin Nutrition 0.000 abstract description 8
- 239000011782 vitamin Substances 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 230000006872 improvement Effects 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- 229930003451 Vitamin B1 Natural products 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 229930014626 natural product Natural products 0.000 description 3
- 229960003495 thiamine Drugs 0.000 description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 3
- 235000010374 vitamin B1 Nutrition 0.000 description 3
- 239000011691 vitamin B1 Substances 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 229940014144 folate Drugs 0.000 description 2
- 125000003929 folic acid group Chemical group 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MSTNYGQPCMXVAQ-KIYNQFGBSA-N 5,6,7,8-tetrahydrofolic acid Chemical compound N1C=2C(=O)NC(N)=NC=2NCC1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 MSTNYGQPCMXVAQ-KIYNQFGBSA-N 0.000 description 1
- 206010002065 Anaemia megaloblastic Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 240000000731 Fagus sylvatica Species 0.000 description 1
- 235000010099 Fagus sylvatica Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001534813 Hypsizygus Species 0.000 description 1
- 240000005856 Lyophyllum decastes Species 0.000 description 1
- 235000013194 Lyophyllum decastes Nutrition 0.000 description 1
- 208000000682 Megaloblastic Anemia Diseases 0.000 description 1
- 241000227425 Pieris rapae crucivora Species 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 206010000891 acute myocardial infarction Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000037354 amino acid metabolism Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100001016 megaloblastic anemia Toxicity 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000002686 mushroom body Anatomy 0.000 description 1
- 210000000276 neural tube Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D475/00—Heterocyclic compounds containing pteridine ring systems
- C07D475/02—Heterocyclic compounds containing pteridine ring systems with an oxygen atom directly attached in position 4
- C07D475/04—Heterocyclic compounds containing pteridine ring systems with an oxygen atom directly attached in position 4 with a nitrogen atom directly attached in position 2
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses an application of a hypsizigus marmoreus strain in preparation of folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCXH-2, and the preservation number is CCTCC NO: m2020068, the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is the China center for type culture Collection. The hypsizigus marmoreus XCXH-2 provided by the invention has the advantages that the folic acid content is obviously higher than that of other varieties, the influence of other water-soluble vitamins is avoided, the extraction purity is high, and the hypsizigus marmoreus XCXH-2 can be used as a natural source for folic acid extraction.
Description
Technical Field
The invention belongs to the technical field of edible fungus strains, and particularly relates to an application of a hypsizigus marmoreus strain in preparation of folic acid.
Background
Hypsizigus marmoreus (Hypsizygusguisammoreus), the scientific name Hypsizigus marmoreus, and wild Hypsizigus marmoreus are mainly distributed in Japan, Europe, North America, and the like. Artificial cultivation of hypsizigus marmoreus has been successfully achieved in japan, korea, china, etc., and artificially cultivated hypsizigus marmoreus products have been commercialized. The hypsizigus marmoreus is widely popular with Asian people because of the fleshy fruiting body, crisp and tender stipe, and sweet and delicious taste.
The common white beech mushroom (white Hypsizygus giganteus) on the market is a white line of the hypsizigus marmoreus, called as 'golden branch jade leaves' in the hypsizigus marmoreus, and the mushroom body is crisp, tender, fresh and smooth, fresh, sweet and delicious; hypsizygus marmoreus (Xieweiguu), also known as Hypsizygus marmoreus, Lyophyllum decastes, is a famous rare Hypsizygus marmoreus, is a gray strain of Hypsizygus marmoreus, has a gray pileus, has a long stipe, has a gray upper portion of the stipe, is introduced from Japan, and has a unique crab flavor; hypsizygus marmoreus (Haixiangu) is a pure white strain of Hypsizygus marmoreus, and has longer stipe than Hypsizygus marmoreus, delicate taste, fragrant smell and delicious taste.
The strains used by the domestic hypsizigus marmoreus manufacturers at present are basically from Japan, and the dominant strains of the hypsizigus marmoreus currently used are numbered wz-29, wz-65 and wz-77; the fruiting performance of the strain wz-29 is good in quality and relatively low in yield per unit; wz-65 and wz-77 have relatively high yield per unit, and the mushroom has a cap with a tumor, short shelf life and relatively poor quality.
Folic acid refers to tetrahydrofolic acid and derivatives thereof, is a water-soluble B vitamin, and is an essential component of organisms. Folic acid is involved in nucleotide biosynthesis, amino acid metabolism and methylation cycle. Folate and the synthetic pathway are critical for cellular metabolism. The deficiency of folic acid causes a number of health problems, such as neural tube malformations in newborns, cancer, cardiovascular disease, megaloblastic anemia, arteriosclerosis, acute myocardial infarction, and stroke. The human body has no ability to synthesize folic acid by itself and can only take it by diet. To meet the needs of normal life activities, adults should be supplemented with 400 μ g of folic acid per day, while pregnant women should be supplemented with 600 μ g per day.
Recently, as consumers attach importance to health, preference for substances extracted from natural materials rather than chemical synthesis has increased.
Disclosure of Invention
The invention aims to provide the application of a specific hypsizigus marmoreus strain in preparing folic acid, folic acid can be conveniently and quickly extracted from the hypsizigus marmoreus strain, and the extraction purity of the folic acid is high.
In order to achieve the technical purpose, the technical scheme provided by the invention is as follows:
an application of hypsizigus marmoreus strain in preparing folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCXH-2 with the preservation number of CCTCC NO: m2020068, the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is the China center for type culture Collection.
According to the detection result, the folic acid content of the hypsizigus marmoreus strain is not less than 56.8 mug per 100g hypsizigus marmoreus strain.
In another aspect, the present invention also provides a method for preparing folic acid, comprising the steps of:
(1) drying and pulverizing Hypsizigus marmoreus, wherein the strain of Hypsizigus marmoreus is Hypsizigus marmoreus XCXH-2, and the preservation number is CCTCC NO: m2020068, wherein the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is China center for type culture Collection;
(2) adding water and organic acid into Hypsizygus marmoreus, heating under high pressure for hydrolysis to obtain extract;
(3) concentrating and drying the extract.
The hypsizigus marmoreus XCXH-2 selected as a natural product has higher folic acid content and extremely low contents of other vitamins, particularly water-soluble vitamin C and vitamin B1, so the hypsizigus marmoreus can be extracted by a simpler extraction process and has high extraction purity.
As a further improvement of the technical proposal, the organic acid in the step (2) is formic acid or acetic acid. Formic acid or acetic acid can stabilize the structure of folic acid in the extraction process, prevent the structure of folic acid from being damaged by heating influence, and improve the extraction yield of folic acid.
As a further improvement of the technical scheme, the weight ratio of the hypsizigus marmoreus and the water in the step (2) is 1: 10-20. If the amount of water used is too small, folic acid cannot be fully extracted; if the amount of water used is too much, folic acid is diluted in water and has low concentration, and the subsequent concentration and drying take longer, so that the production efficiency is reduced.
As a further improvement of the technical scheme, the weight ratio of the water to the organic acid in the step (2) is 100: 1-5. If the content of the organic acid is too low, the organic acid does not function sufficiently; if the organic acid content is too high, the acidity of the whole extract becomes strong and folic acid may be destroyed.
As a further improvement of the technical scheme, in the step (2), the heating pressure is 0.1-0.12 Mpa, the heating temperature is 80-100 ℃, and the heating time is 2-5 hours. If the heating temperature is lower than 80 ℃, folic acid is not fully extracted; if the heating temperature exceeds 90 c or the heating time is too long, the structure of folic acid may be broken.
According to the invention, through detection research on specific hypsizigus marmoreus XCXH-2, the content of folic acid contained in the hypsizigus marmoreus is high, the content of other water-soluble vitamins is low, and when the hypsizigus marmoreus is applied to folic acid extraction, the extraction process is simple and the purity is high.
Drawings
FIG. 1 is a photograph of harvest time of Hypsizigus marmoreus XCXH-2.
Detailed Description
The following examples are provided to further illustrate embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the respective embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and biomaterials, if not specifically indicated, are commercially available.
Example 1: content detection of hypsizigus marmoreus XCXH-2 folic acid and other vitamins
Collecting Hypsizigus marmoreus XCXH-2 (shown in figure 1) at harvesting stage, and sending to Hua-testing and certification group, GmbH, for detecting folic acid and other vitamins. The detection methods of the substances were carried out according to the detection standards specified in the industry, and the detection results are shown in table 1 below.
Table 1: hypsizigus marmoreus XCXH-2 folate and other vitamin content
As can be seen from the detection results, the content of folic acid in the hypsizigus marmoreus XCXH-2 is higher, but the content of other vitamins, particularly water-soluble vitamin C and vitamin B1 is extremely low, so that the hypsizigus marmoreus XCXH-2 is very suitable to be used as a natural product source for extracting folic acid.
Example 2: extraction of Hypsizigus marmoreus XCXH-2 folic acid
(1) Weighing and cutting dried hypsizigus marmoreus XCXH-2(100g), wherein the preservation number of the hypsizigus marmoreus XCXH-2 is CCTCC NO: m2020068, the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is the China center for type culture Collection.
(2) Adding 1500ml distilled water into Hypsizigus marmoreus, and immersing Hypsizigus marmoreus XCXH-2. If the amount of water used is too small, folic acid cannot be fully extracted; if the amount of water used is too much, folic acid is diluted in water and has low concentration, and the subsequent concentration and drying take longer, so that the production efficiency is reduced.
(3) Further adding 7.5ml acetic acid, and mixing well. The acetic acid can stabilize the folic acid structure in the extraction process, prevent the folic acid structure from being damaged by heating influence, and improve the extraction yield of folic acid. If the content of the organic acid is too low, the organic acid does not function sufficiently; if the organic acid content is too high, the acidity of the whole extract becomes strong and folic acid may be destroyed.
(4) The mixture was heated to 80 ℃ under 0.1MPa for 2 hours. If the heating temperature is lower than 80 ℃, folic acid is not fully extracted; if the heating temperature exceeds 90 c or the heating time is too long, the structure of folic acid may be broken.
(5) Concentrating and drying the extract. The concentration and drying means may be vacuum concentration, freeze-drying concentration, etc. which are commonly used in the art.
The hypsizigus marmoreus XCXH-2 selected as a natural product has higher folic acid content and extremely low contents of other vitamins, particularly water-soluble vitamin C and vitamin B1, so the hypsizigus marmoreus can be extracted by a simpler extraction process and has high extraction purity.
The embodiments of the present invention have been described in detail with reference to experimental data, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (7)
1. An application of hypsizigus marmoreus strain in preparing folic acid, wherein the hypsizigus marmoreus strain is hypsizigus marmoreus XCXH-2 with the preservation number of CCTCC NO: m2020068, the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is the China center for type culture Collection.
2. The use as claimed in claim 1, wherein the folic acid content of the hypsizigus marmoreus strain is not less than 56.8 μ g per 100 g.
3. A method of preparing folic acid comprising the steps of:
(1) drying and pulverizing Hypsizigus marmoreus, wherein the strain of Hypsizigus marmoreus is Hypsizigus marmoreus XCXH-2, and the preservation number is CCTCC NO: m2020068, wherein the preservation date is 2020, 4 and 17 months, the preservation address is Wuchang Lojia mountain in Wuhan city, Hubei province, and the preservation unit is China center for type culture Collection;
(2) adding water and organic acid into Hypsizygus marmoreus, heating under high pressure for hydrolysis to obtain extract;
(3) concentrating and drying the extract.
4. The process for producing folic acid according to claim 3, wherein the organic acid in the step (2) is formic acid or acetic acid.
5. A process for producing folic acid according to claim 4, wherein the weight ratio of the hypsizygus marmoreus and the water in the step (2) is 1:10 to 20.
6. The process for producing folic acid according to claim 5, wherein the weight ratio of water and the organic acid in the step (2) is 100:1 to 5.
7. The method of producing folic acid according to claim 3, wherein the heating pressure in the step (2) is 0.1 to 0.12MPa and the heating temperature is 80 to 100 ℃.
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2021
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