CN106619755A - Preparation method and preparation of natural moringa oleifera folic acid - Google Patents

Preparation method and preparation of natural moringa oleifera folic acid Download PDF

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Publication number
CN106619755A
CN106619755A CN201611220581.5A CN201611220581A CN106619755A CN 106619755 A CN106619755 A CN 106619755A CN 201611220581 A CN201611220581 A CN 201611220581A CN 106619755 A CN106619755 A CN 106619755A
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folic acid
preparation
natural
moringa
extract
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田洋
盛军
杨扬
彭磊
代佳和
史崇颖
戴天浥
杨明
贺水莲
戴吴兰
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Yunnan Agricultural University
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Yunnan Agricultural University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a preparation method and a preparation of natural moringa oleifera folic acid, belonging to the technical fields of food additives, nutrient health products and medicines. The natural moringa oleifera folic acid prepared by virtue of the preparation method is a composition containing tetrahydrofolic acid, 5-formyl tetrahydrofolic acid and 5-methyl tetrahydrofolic acid in a certain proportion; moringa oleifera leaf powder contains about 10 micrograms per gram of total natural folic acid, the content of the natural folic acid in a purified extract reaches up to 258 micrograms per gram without adding any organic reagent and enzyme by virtue of an optimized preparation method, and compared with the content of the natural folic acid in the raw materials, the content of the natural folic acid is increased by 25.8 times, so that the requirements on the content of folic acid in various health products, foods or additives in the current market; and besides, a passion fruit, sweet tea or wolfberry fruit water extract with natural antioxidant and preservation functions is added into the extracted raw materials, so that the chemical stability of a moringa oleifera folic acid extract is enhanced, and meanwhile, the content of the natural folic acid in the moringa oleifera folic acid extract is further increased.

Description

A kind of preparation method and preparation of the natural folic acid of Moringa
Technical field
The invention belongs to food additives, nutrient and healthcare products and pharmaceutical technology field, the natural leaf of more particularly to a kind of Moringa The preparation method and preparation of acid.
Background technology
Moringa is a kind of torrid zone, subtropical plant, rich in various nutrition such as vegetable protein, vitamin, pantothenic acid, calcium, iron, selenium Material, wherein, the content of protein is 2 times of milk, and the content of calcium is 4 times of milk, and ascorbic content is the 7 of orange Times, the content of vitamin A is 4 times of carrot, and the content of iron is 3 times of spinach, and the content of potassium is 3 times of banana, gamma-amino The content of butyric acid is 10 times of fermentation profound rice；Also contain natural folic acid, be 3-10 times of other fruits and vegetables, reach as high as 1100 μ g/ 100g。
Folic acid belongs to B family vitamins, is to maintain a type organic necessary to organism normal living process, can help egg White matter metabolism, and and Cobastab12Generation and the maturation of red blood cell are collectively promoted, is the indispensable material of manufacture red blood cell.Together When folic acid also have antitumor, promote infant's nerve cell and brain cell development, treatment of vascular hardening and the effect blocked.Generation Boundary's health organization is to the daily recommended amounts of folic acid：Adult's consumption is 400 μ g for the consumption of 200 μ g, pregnant woman and wet nurse；The U.S. pair The daily recommended amounts of folic acid are：Adult's consumption is 400 μ g.
The important physiological action of folic acid is the carrier participation metabolism as one-carbon compound.The derivative tetrahydrofolic acid of folic acid Carrier with co-enzyme form as one carbon unit (including formyl, formaldehyde and methyl), the transfer and formyl and formaldehyde to methyl Utilization have important function.The biosynthesis of body adenylic acid, chest pyrimidine nucleotide, serine is mutual with glycine Become, the biosynthesis of glutamic acid and choline, homocystine is converted into methionine and is required for tetrahydrofolic acid.Folic acid coenzyme promotes fast Purine, the biosynthesis of chest pyrimidine nucleotide and methionine, serine, the conversion of glutamic acid, change, illustrate life of the folic acid in nucleic acid Thing synthesizes the importance in the biosynthetic process with protein.This has also absolutely proved folic acid in cell growth and regeneration Important function.
At present, the food addition folic acid and folic acid health products composition applied on market is all from synthesizing folic acid, synthesis Folic acid is stable in properties, and synthetic route is ripe cheap, but compared with natural folic acid, tetrahydrochysene reductase is made in its metabolism palpus Jing liver With can just be converted into the absorbable tetrahydrofolic acid for utilizing, Jing informal dresses increase liver physiology burden with easy.And tetrahydrochysene is also in human body Protoenzyme lacks, and the effective bioavilability for synthesizing folic acid is relatively low, and long-term taking is also possible to that human body Zn-ef ficiency metabolic disorder can be caused, The bad reaction of the organ such as skin, stomach is caused to occur, excessive use synthesis folic acid can also increase suffers from the diseases such as colon cancer, breast cancer The risk of disease.The folic acid of natural origin is the reduction-state form structure of synthesis folic acid (including tetrahydrofolic acid, 5- methyl tetrahydrochysene leaves Many glutamic acid substitution products of acid, 5- formoxyl tetrahydrofolic acids, 10- formoxyls tetrahydrofolic acid and tetrahydrofolic acid), its structure type Determine that natural folic acid has more excellent bioavilability and security compared with synthesis folic acid.Therefore, research and develop a kind of natural Folic acid becomes the technical problem of urgent need to resolve.
The content of the invention
The invention aims to overcome the deficiencies in the prior art, and provide a kind of preparation side of the natural folic acid of Moringa Method, the natural folic acid of preparation method gained is safe and reliable, suitable long-term taking.
The object of the present invention is achieved like this, a kind of preparation method of the natural folic acid of Moringa, comprises the following steps：Take peppery Wood, plus pure water extraction under conditions of temperature is for 20 DEG C～100 DEG C, filter, by filtrate concentrate drying.
Preferably, the Moringa includes the one kind in fresh leaf of Moringa, Moringa limb or leaf of Moringa powder, or they appoint Meaning scalemic thereof；Every time the addition weight of the pure water is 10～80 times of the Moringa weight；The number of times of the extraction is 1 ～4 times, the time extracted every time is 15～120 minutes；The mode of the extraction is carried out by the way of ultrasonic wave extraction；Institute State drying is carried out using cryodesiccated mode.The plant capacity of ultrasonic wave extraction can be 500-3000 watt.
Preferably, while the ultrasonic wave extraction, when extracting mechanical agitation is carried out；During the filtration, first carry out Centrifugation, gained supernatant is filtered again using the one kind in microfiltration membranes, milipore filter, NF membrane or reverse osmosis membrane.During centrifugation Rotating speed can be 1000-5000 rev/min.
Preferably, the microfiltration membranes are filter membrane that aperture is 0.1～0.8 μm；The milipore filter is to allow 5～10 very much The filter membrane that son amount is passed through；The NF membrane is to allow the filter membrane that the molecule of 300～1000 molecular weight is passed through.
Preferably, folic acid in make fresh leaf of Moringa is stablized, the fresh leaf of Moringa after plucking 12 hours with Interior fresh leaf is preserved under conditions of being placed in -80 DEG C～0 DEG C.
Preferably, during described plus Aqua pure extract, adding the one kind in passion fruit, Sweet tea or Lycium chinense fruit aqueous extract, or hundred Fragrant fruit, Sweet tea and Lycium chinense fruit aqueous extract mass ratio are 1:1:1 mixture.
Preferably, the addition weight of the passion fruit, Sweet tea or Lycium chinense fruit aqueous extract be the Moringa weight 3%～ 8%；The Sweet tea is selected from frying Sweet tea；The fruit of Chinese wolfberry is selected from lycium ruthenicum.
Present invention also offers a kind of natural folic acid extract obtained by the preparation method, the natural folic acid extraction Total folate content is 60 μ g/g～278 μ g/g in thing；Wherein, the natural folic acid dependency structure derivative content of contained certain proportion is most Height is respectively：5- formoxyls tetrahydrofolic acid is 136 μ g/g, and methyl tetrahydrofolate is 50 μ g/g, and tetrahydrofolic acid is 92 μ g/g (numbers According to the test from the products obtained therefrom of embodiment 10).
Present invention also offers a kind of preparation of natural folic acid extract, the preparation is oral formulations, injection, suppository Or inhalant.When corresponding preparation is prepared, also including pharmaceutically acceptable auxiliary material.
Preferably, the oral formulations be tablet, capsule, granule, pulvis, microcapsules, pill, decoction, paste, Dispersion powders, distillate medicinal water, oral liquid, pill or liposome.
Preferably, the injection is powder-injection or parenteral solution.
Preferably, the pharmaceutically acceptable accessory package contains Fruit powder, flavoring essence, sweetener, acid, filling In agent, lubricant, preservative, suspending agent, food coloring, diluent, emulsifying agent, disintegrant or plasticizer one or two with On mixture.
Preferably, Fruit powder is one or both of orange powder, orange powder, lemon powder, cherry powder, apple powder or coconut powder Mixture above.But those skilled in the art think feasible Fruit powder within protection scope of the present invention, Fruit powder Species is not limited thereto, and here of the present invention is not limited.
Preferably, flavoring essence is flavoring orange essence, orange essence, lemon extract, cherry essence, menthol, flavoring apple essence Or mixture more than one or both of coconut essence.But those skilled in the art think feasible flavoring essence at this Within bright protection domain, flavoring essence species is not limited thereto, and here of the present invention is not limited.
To increase the sugariness of medicine, it is easy to take.Preferably, sweetener is Sucralose, 3,4-Dihydro-6-methyl-1,2,3-oxathiazin-4-one 2,2-dioxide potassium salt, Aspartame or sieve Mixture more than one or both of sweet glycosides of Chinese fruit.But those skilled in the art think feasible sweetener in the present invention Protection domain within, sweetener species is not limited thereto, and here of the present invention is not limited.
Preferably, acid is mixing more than one or both of citric acid, malic acid, lactic acid or citric acid Thing.But those skilled in the art think feasible acid within protection scope of the present invention, acid species not office It is limited to this, here of the present invention is not limited.
Filler refers to increase the weight and volume of tablet, beneficial to the auxiliary material of compressing tablet.Filler as tablet will Ask mobility, compressibility good, adhesion is strong, there is larger saturation to medicine.Preferably, filler be fructose, glycitols, Mixture more than one or both of sucrose, starch, pregelatinized starch, microcrystalline cellulose or dextrin.But art technology Personnel think feasible disintegrant within protection scope of the present invention, and filler species is not limited thereto, and the present invention exists This is not limited.
Lubricant refers to the auxiliary material that can reduce frictional force between tablet and die wall, to prevent frictional force big is stranded compressing tablet Difficulty, pressure distribution is uniform when can make compressing tablet, and makes the even density of tablet, can also improve the outward appearance of tablet, makes tablet surface It is bright, smooth.Preferably, lubricant is more than one or both of talcum powder, magnesium stearate, silica or stearic acid Mixture.But those skilled in the art think feasible lubricant within protection scope of the present invention, lubricant species It is not limited thereto, here of the present invention is not limited.
Preferably, preservative is Sodium Benzoate, Potassium Benzoate, sorbic acid methyl esters, ethyl-para-hydroxybenzoate or to hydroxyl Mixture more than one or both of yl benzoic acid phenyl ester.But those skilled in the art think feasible preservative at this Within the protection domain of invention, preservative species is not limited thereto, and here of the present invention is not limited.
Preferably, suspending agent is more than one or both of sodium carboxymethylcellulose, sodium alginate or beeswax mixed Compound.But those skilled in the art think feasible suspending agent within protection scope of the present invention, and suspending agent species is not This is confined to, here of the present invention is not limited.
Preferably, food coloring is more than one or both of caramel colorant, Gardenia Yellow, curcumin or chlorophyll Mixture.But those skilled in the art think feasible food coloring within protection scope of the present invention, food coloring Species is not limited thereto, and here of the present invention is not limited.
Preferably, diluent be edible vegetable oil, propane diols or molecular weight be in 400～6000 polyethylene glycol one Mixture more than kind or both.But those skilled in the art think feasible diluent protection scope of the present invention it Interior, diluent species is not limited thereto, and here of the present invention is not limited.
Preferably, emulsifying agent is S-40, stearoyl lactate/calcium, diacetyl tartarate monoglyceride, sucrose-fatty Ester, molecular weight are mixture more than one or both of 400～6000 polyethylene glycol or distillation monoglyceride.But this area Technical staff thinks feasible emulsifying agent within protection scope of the present invention, and emulsifier is not limited thereto, this Bright here is not limited.
The Main Function of disintegrant is to eliminate the adhesion of tablet is formed because of adhesive or by pressurization to make tablet rapid Disintegration, enables tablet to play drug effect rapidly.Preferably, disintegrant is dried starch, sodium carboxymethyl starch, low substituted hydroxy-propyl fibre Mixture more than one or both of dimension element, PVPP or sodium carboxymethylcellulose.But this area skill Art personnel think feasible disintegrant within protection scope of the present invention, and filler species is not limited thereto, the present invention Here is not limited.
Preferably, plasticizer be glycerine, sodium carboxymethylcellulose, sorbierite or oleamide sodium sulfonate in one kind or Mixture more than both.But those skilled in the art think feasible plasticizer within protection scope of the present invention, increase Modeling agent species is not limited thereto, and here of the present invention is not limited.
Using above-mentioned technical proposal, due to directly obtaining natural folic acid, 5- formoxyls in the natural folic acid of gained from Moringa , up to 136 μ g/g, up to 50 μ g/g, the content of tetrahydrofolic acid is up to 92 μ for the content of methyl tetrahydrofolate for the content of tetrahydrofolic acid g/g.Compare synthesis folic acid, the natural folic acid of Moringa it is safe, toxic and side effect is little, can be taken for a long time.The present invention is also There is provided the preparation method of the natural folic acid, and the preparation prepared by the natural folic acid.The preparation method is used at a certain temperature Aqua pure extract, the method that gained active material is extracted apparently higher than prior art optimizes the composition of natural folic acid so that natural Folic acid more easily absorption of human body simultaneously plays its physiological function.
Specific embodiment
The specific embodiment of the present invention is described further below.Here is it should be noted that for these enforcements The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.Additionally, invention described below As long as involved technical characteristic does not constitute each other conflict and just can be mutually combined in each embodiment.
Embodiment 1
A kind of preparation method of the natural folic acid of Moringa, comprises the following steps：Leaf of Moringa powder is taken, plus pure water is 80 DEG C in temperature Under conditions of extract, filter, filtrate is concentrated, freeze-drying.Wherein, the number of times of extraction is 1 time, and the time extracted every time is 30 Minute；Every time the addition weight of pure water is 20 times of the Moringa weight；The mode of extraction by the way of ultrasonic wave extraction come Carry out；Drying is carried out using cryodesiccated mode.While ultrasonic wave extraction, when extracting mechanical agitation is carried out；Filter When, first it is centrifuged, gained supernatant is carried out again using the one kind in microfiltration membranes, milipore filter, NF membrane or reverse osmosis membrane.Its In, microfiltration membranes are filter membrane that aperture is 0.1～0.8 μm；The milipore filter is to allow the filter membrane that 5～100,000 molecular weight are passed through；Institute It is to allow the filter membrane that the molecule of 300～1000 molecular weight is passed through to state NF membrane.
Embodiment 2-5
The temperature of extraction is changed to into respectively 20 DEG C, 40 DEG C, 60 DEG C or 100 DEG C by 80 DEG C, remaining preparation condition is pressed completely Carry out according to the mode of embodiment 1, constitute embodiment 2-5.
The natural folate content testing result that embodiment 1-5 is obtained, is shown in Table 1.
Impact of the different Extracting temperatures of table 1 to natural folate content
Product Extracting temperature Natural folic acid
Embodiment 1 80℃ 208μg/g
Embodiment 2 20℃ 60μg/g
Embodiment 3 40℃ 115μg/g
Embodiment 4 60℃ 208μg/g
Embodiment 5 100℃ 160μg/g
Embodiment 6-8
The number of times of extraction is changed to respectively 2 times, 3 times or 4 times by 1 time, remaining enters fully according to the mode of embodiment 1 OK, embodiment 6-8 is constituted.
The natural folate content testing result that embodiment 6-8 is obtained, is shown in Table 2.
Impact of the different extraction times of table 2 to natural folate content
Product Extraction time Natural folic acid
Embodiment 6 2 163μg/g
Embodiment 7 3 152μg/g
Embodiment 8 4 135μg/g
Embodiment 9-12
The time of extraction was changed to respectively 15 minutes, 60 minutes, 120 minutes or 180 minutes by 30 minutes, remaining is complete Carry out according to the mode of embodiment 1, constitute embodiment 9-12.
The natural folate content testing result that embodiment 9-12 is obtained, is shown in Table 3.
Impact of the different extraction times of table 3 to natural folate content
Product Extraction time Natural folic acid
Embodiment 9 15 minutes 136μg/g
Embodiment 10 60 minutes 258μg/g
Embodiment 11 120 minutes 155μg/g
Embodiment 12 180 minutes 97μg/g
Embodiment 13-15
The addition weight of each pure water is changed to into respectively 10 times, 40 times or 80 times by 20 times, remaining is fully according to enforcement The mode of example 1 constitutes embodiment 13-15 carrying out.
The natural folate content testing result that embodiment 13-15 is obtained, is shown in Table 4.
Impact of the different extraction times of table 4 to natural folate content
Product The addition weight of pure water Natural folic acid
Embodiment 13 10 times 126μg/g
Embodiment 14 40 times 137μg/g
Embodiment 15 80 times 198μg/g
Integrated embodiment 1-15, can show that the optimum condition extracted to the natural folic acid of Moringa is combined as：80 DEG C of Extracting temperature, Extraction time 1 time, 60 minutes extraction times and Extraction solvent (pure water) addition are 20 times of weight.
Embodiment 16-19
Plus during Aqua pure extract, add the one kind in passion fruit, Sweet tea or Lycium chinense fruit aqueous extract, or passion fruit, Sweet tea with Lycium chinense fruit aqueous extract mass ratio is 1:1:1 mixture.Wherein, the addition weight of passion fruit, Sweet tea or Lycium chinense fruit aqueous extract is The 5% of the Moringa weight；The Sweet tea is selected from frying Sweet tea；The fruit of Chinese wolfberry is selected from lycium ruthenicum.Remaining is fully according to enforcement The mode of example 1 constitutes embodiment 16-19 carrying out.
Embodiment 1, the natural folate content testing result that 16-19 is obtained, is shown in Table 5.
Table 5 adds impact of the different materials to natural folate content and shelf-life
From table 5, under identical extraction conditions, different materials are added, can be to the content of natural folic acid and its composition There is significant impact, achieve unforeseeable technique effect.
In the present invention, frying Sweet tea just Sweet tea is placed in be stir-fried 45 minutes in the frying pan that temperature is 90 DEG C and obtains；The fruit of Chinese wolfberry Water extract is to add 10 times of amount decoctings to boil 2 hours lycium ruthenicum, is filtered, and concentrate drying is obtained.
Embodiment 20
Natural folic acid product obtained by Example any one of 1-19, adds appropriate superfine silica gel powder, fills capsule, obtains final product glue Wafer.
Embodiment 21
Natural folic acid product obtained by Example any one of 1-19, adds the appropriate orange powder of appropriate addition, orange essence, sucrose Mix, compressing tablet obtains final product tablet.
Embodiment 22
Natural folic acid product obtained by Example any one of 1-19, adds suitable amount of sucrose powder, mixes, and granulation obtains particle Agent.
Embodiment 23
Natural folic acid product obtained by Example any one of 1-19, adds water dissolves, adds appropriate Aspartame, Jiao Fried sugar element, citric acid and Sodium Benzoate, mix, and filter, and bottling obtains oral solutions.
Embodiment 24
Natural folic acid product obtained by Example any one of 1-19, adds water for injection dissolving, adds appropriate amount of addition agent, Routinely preparation method, makes injection.
Embodiment 25
Natural folic acid product obtained by Example any one of 1-19, adds appropriate amount of addition agent, and routinely preparation method, makes bolt Agent.
Embodiment 26
Natural folic acid product obtained by Example any one of 1-19, adds appropriate amount of addition agent, and routinely preparation method, makes suction Agent.
Embodiments of the present invention are explained in detail above, but the invention is not restricted to described embodiment.It is right For those skilled in the art, in the case of without departing from the principle of the invention and spirit, these embodiments are carried out many Plant change, modification, replace and modification, still fall within protection scope of the present invention.

Claims (9)

1. the preparation method of the natural folic acid of a kind of Moringa, it is characterised in that comprise the following steps：Moringa, plus pure water are taken in temperature To extract under conditions of 20 DEG C～100 DEG C, filter, by filtrate concentrate drying.
2. the preparation method of the natural folic acid of a kind of Moringa according to claim 1, it is characterised in that the Moringa is comprising fresh One kind in leaf of Moringa, Moringa limb or leaf of Moringa powder, or their arbitrary proportion mixture；The addition weight of each pure water Measure as 10～80 times of the Moringa weight；The number of times of the extraction is 1～4 time, and the time extracted every time is 15～120 points Clock；The mode of the extraction is carried out by the way of ultrasonic wave extraction；The drying is carried out using cryodesiccated mode.
3. the preparation method of the natural folic acid of a kind of Moringa according to claim 2, it is characterised in that the ultrasonic wave extraction Meanwhile, carry out mechanical agitation when extracting；During the filtration, first it is centrifuged, gained supernatant adopts again microfiltration membranes, ultrafiltration One kind in film, NF membrane or reverse osmosis membrane is filtered.
4. the preparation method of the natural folic acid of a kind of Moringa according to claim 3, it is characterised in that the microfiltration membranes are aperture For 0.1～0.8 μm of filter membrane；The milipore filter is to allow the filter membrane that 5～100,000 molecular weight are passed through；The NF membrane is to allow The filter membrane that the molecule of 300～1000 molecular weight is passed through.
5. a kind of preparation method of the natural folic acid of Moringa according to claim 1, it is characterised in that the fresh leaf of Moringa choosing Fresh leaf from after harvesting within 12 hours is preserved under conditions of being placed in -80 DEG C～0 DEG C.
6. a kind of preparation method of the natural folic acid of Moringa according to claim 1, it is characterised in that described plus Aqua pure extract When, add the one kind in passion fruit, Sweet tea or Lycium chinense fruit aqueous extract, or passion fruit, Sweet tea and Lycium chinense fruit aqueous extract mass ratio to be 1:1:1 mixture.
7. the preparation method of the natural folic acid of a kind of Moringa according to claim 6, it is characterised in that the passion fruit, Sweet tea Or 3%～8% that the addition weight of Lycium chinense fruit aqueous extract is the Moringa weight；The Sweet tea is selected from frying Sweet tea；The Chinese holly Matrimony vine is selected from lycium ruthenicum.
8. a kind of natural folic acid extract obtained based on preparation method described in any one of claim 1-7, the natural folic acid The content of natural folic acid is 60 μ g/g～258 μ g/g in extract.
9. a kind of preparation comprising natural folic acid extract as claimed in claim 8, it is characterised in that the preparation is oral Preparation, injection, suppository or inhalant.
CN201611220581.5A 2016-12-26 2016-12-26 Preparation method and preparation of natural moringa oleifera folic acid Pending CN106619755A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113582996A (en) * 2021-06-02 2021-11-02 韶关市星河生物科技有限公司 Application of hypsizigus marmoreus strain in preparation of folic acid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
R.K.SAINI等: "Relative bioavailability of folate from the traditional food plant Moringa oleifera L. as evaluated in a rat model", 《JOURNAL OF FOOD SCIENCE AND TECHNOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113582996A (en) * 2021-06-02 2021-11-02 韶关市星河生物科技有限公司 Application of hypsizigus marmoreus strain in preparation of folic acid
CN113582996B (en) * 2021-06-02 2024-02-06 韶关市星河生物科技有限公司 Application of hypsizigus marmoreus strain in preparation of folic acid

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