KR101980144B1 - A composition for antioxidating, whitening and improving wrinkle comprising extracts of pleurotus salmoneostramineus - Google Patents

Abstract

The present invention relates to a composition containing a Pleurotus salmoneostramineus extract. The composition containing the Pleurotus salmoneostramineus extract as an active ingredient has a high total phenol content, an excellent antioxidant effect due to excellent DPPH free radical scavenging activities, a tyrosinase inhibitory effect, and thus a whitening effect as well as a wrinkle reducing effect by inhibiting collagenase activities. In addition, the Pleurotus salmoneostramineus extract of the present invention has no cytotoxicity and skin side effects and can be used safely in cosmetic, pharmaceutical and food compositions.

Description

TECHNICAL FIELD The present invention relates to a composition for antioxidant, whitening and wrinkle containing a pink oyster extract, and to a composition for improving whitening and wrinkles. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for antioxidant,

The present invention relates to a composition containing Pleurotus salmoneostramineus extract, and more particularly to a cosmetic, a pharmaceutical composition and a food composition for antioxidation, whitening and wrinkle improvement containing an extract of pink ointment as an active ingredient .

Environmental pollution causes various diseases in the human body. Climatic changes due to environmental pollution are increasingly exposed to heavy metals and toxic substances along with fine dust, dust, and ultraviolet rays. Especially, the skin that surrounds our body is the primary body shield and the most adversely affected by external stimuli. Therefore, there is an increasing interest in protecting the skin from irritants and keeping it healthy and there is an increasing tendency to develop products using natural resources.

The human skin consists of the epidermis, dermis, and connective tissue including the stratum corneum, and the stratum corneum is composed of a dead cell layer formed through the differentiation process of keratinocyte, the basal cell of the epidermis And is responsible for protecting the human body from the effects of the external environment. There are two major researches on the causes of skin aging. One is "internal aging", which is caused by changes in the function of cells, which are constitutive units of skin, with age, and the other is "external aging" Which can be divided into external environment such as ultraviolet rays, pollution, stress and the like.

As the cells of the skin are exposed to oxidative stress by reactive oxygen species generated in a continuous biochemical reaction for the necessary energy supply in the human body in order to supply necessary energy in vivo, the cellular components such as lipid, protein, It changes carbohydrate and DNA oxidative damage and enzymatic activity, causing various diseases such as skin cancer, and promotes aging. In addition, the biggest cause of external aging is photoaging due to ultraviolet rays, which causes acute and chronic skin injuries such as skin cancer through continuous exposure to UV.

UV rays can cause skin pigmentation such as freckles, spots, stains, and black spots, but it can also cause skin aging. Skin aging can be divided into internal aging that occurs naturally as time passes, and external aging that occurs due to the surrounding environment (pollutants, bacteria, stress, etc.). In particular, the skin is exposed to the sun's ultraviolet rays, resulting in the generation of harmful reactive oxygen species (ROS), which are highly reactive with antioxidant enzymes and reduced antioxidants such as vitamins C, E and glutathione. These reactive oxygen species are known to cause melanin production, wrinkle-inducing photoaging, and inhibit DNA, protein oxidation and skin immune functions while damaging skin enzymes and nonenzymatic antioxidant defense systems. To induce an inflammatory reaction. Therefore, there is much interest and research on the development of antioxidants capable of protecting skin cells and alleviating skin aging by removing active oxygen.

The biosynthesis and degradation of collagen, which is one of the important components of the dermal matrix of skin, plays a key role in inhibiting skin aging. The active oxygen species promotes the expression of matrix metalloproteinases (MMPs) in skin fibroblasts, Aging of the skin is promoted by promoting the synthesis of azelaic acid.

On the other hand, human skin color is determined by the concentration and distribution of melanin in the skin. In addition to genetic factors, skin color is also affected by environmental or physiological conditions such as sunlight, fatigue and stress. Melanin is synthesized in melanocytes in the epidermis of the skin. In the melanosome of melanocytes, an enzyme called tyrosinase acts on tyrosine, a type of amino acid, resulting in dopa (DOPA), dopaquinone ), And then it is made through a non-enzymatic oxidation reaction. When such a synthesis of melanin occurs excessively in the skin, it darkens skin tone, and causes spots and freckles. Thus, inhibiting the activity of tyrosinase in the skin to inhibit the synthesis of melanin pigment not only makes it possible to whiten the skin tone to brighten the skin tone, but also to reduce skin irritation caused by ultraviolet rays, hormones and hereditary causes, It can improve constipation.

Accordingly, in the field of cosmetics, research and development on functional cosmetics having antioxidative, whitening and wrinkle-reducing effects have been actively carried out, and studies using natural substances have been continued to prevent toxicity or irritation to the skin.

On the other hand, Pleurotus salmoneostramineus ( Pleurotus salmoneostramineus ) is a white rot fungus belonging to the Pleurotus eryngii family. It is a high temperature mushroom which grows in a large number of hardwood stump or dead wood in summer ~ autumn. It is native to Asia, Europe, America and Africa but not found in Korea. It is called 'pink oyster', 'salmon oyster' and 'flamingo oyster'. When it is young or fresh, it grows pink but turns to light pink. It is known that red antelopein and lycopene are highly antioxidant and have high potassium, calcium, magnesium, phosphorus and iron content and low polysaccharide and crude fat content. Recently, the RDA has succeeded in the development of domestic red mushroom, and has been making efforts for cultivation by establishing cultivation and management technology. However, in spite of its excellent functionality, it has limitations in that it is used only for edible purposes and its application field is very narrow.

Accordingly, the inventors of the present invention have continued to develop new natural substances having antioxidative, whitening effect and wrinkle-reducing effects while reducing the side effects of human body. As a result, it has been found that the pink oyster extract has antioxidative, whitening effect The present invention has been completed.

Accordingly, a technical problem to be solved by the present invention is to provide a substance having antioxidative, whitening and wrinkle-reducing effects, and having excellent human stability.

In order to solve the above-mentioned technical problems, the present invention provides a composition for antioxidant, whitening and wrinkle improvement, which comprises a pink oyster extract as an active ingredient.

Preferably, the composition comprising the pink oyster extract in the present invention may be a cosmetic composition, a pharmaceutical composition or a food composition.

The term " extract " of the present invention means a preparation which is obtained by squeezing a herbal medicine with an appropriate leaching solution and concentrating by evaporating the leaching solution, and is not limited thereto. The extract, the diluted solution, A dried product obtained by drying, a controlled preparation thereof, or a purified product thereof.

The pink oyster extract of the present invention can be prepared by using common extraction, isolation and purification methods known in the art. Examples of the extraction method include, but are not limited to, hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction.

According to an embodiment of the present invention, the pink oyster extract is prepared by pulverizing the shade pink oyster and then extracting a solvent selected from the group consisting of water, an anhydrous or low-boiling alcohol having 1 to 3 carbon atoms, and a mixed solvent thereof, , And the amount of the extraction solvent may be 2 to 20 times by weight, preferably 5 to 15 times by weight, of the dry weight of the herbal medicine.

In the present invention, mushroom angles and mushroom sacks, which are edible parts, can be used for the use area of the pink oyster.

In one specific embodiment of the present invention, the pink Pleurotus ostreatus extract is dried using an organic solvent at a temperature of 30 to 50 DEG C, preferably 40 DEG C for 10 to 30 hours, preferably for 15 to 20 hours And then filtered through a filter paper to obtain a pink oyster extract.

The composition of the present invention may contain 0.005 to 50% by weight, more preferably 0.01 to 30% by weight, and most preferably 0.1 to 10% by weight, based on the total weight of the composition, of pink oyster extract. When the content of the pink oyster extract is less than 0.005% by weight, antioxidative, whitening and wrinkle reducing effects, which are the object of the present invention, can not be obtained. When the content exceeds 50% by weight, the effect is not proportional to the content There is a problem that the stability of the shape can not be secured.

The composition containing the pink oyster extract of the present invention exhibits an antioxidative effect, a whitening effect and a wrinkle-reducing effect, and has little cytotoxicity as a natural substance.

According to one embodiment of the present invention, there is provided a cosmetic composition comprising a pink oyster extract as an active ingredient.

The cosmetic composition according to one embodiment of the present invention may contain, in addition to the pink oyster extract as an active ingredient, conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, The carrier may be further added.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.

When the formulation of the present invention is a paste, cream or gel, use is made of an animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, tosse, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Castellulose, aluminum metahydroxide, bentonite, agar or tracert, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The pharmaceutical composition according to one embodiment of the present invention includes a pharmaceutically acceptable carrier other than the pink Pleurotus extract. The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one usually used at the time of formulation, and includes lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably applied by parenteral administration, more preferably topical application by application.

A suitable dosage of the pharmaceutical composition of the present invention may vary depending on such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition of the present invention is in the range of 0.001-100 mg / kg on an adult basis. When the composition is an external preparation, it is preferably applied in an amount of 1.0 to 3.0 ml on an adult basis once to five times a day, and continued for 1 month or more. However, the dose is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art, Into the container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in an oil or aqueous medium, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

In addition, the food composition according to one embodiment of the present invention may contain not only the pink oyster extract as an active ingredient, but also components commonly added in the manufacture of food such as protein, carbohydrate, fat, nutrients, May be further included.

Examples of such carbohydrates are monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, .

On the other hand, since the pink oyster extract of the present invention is a natural substance, it is harmless to the human body and has little toxicity and side effects, so that it can be safely used for a long period of use and can be safely applied to cosmetic, pharmaceutical and food compositions as described above .

Thus, the composition comprising the pink oyster extract of the present invention as an active ingredient has a high total phenol content, an excellent antioxidative effect due to the excellent DPPH free radical scavenging activity, a tyrosinase inhibitory effect and a whitening effect, By inhibiting the activity of the genase, it has a wrinkle-reducing effect. In addition, the pink oyster extract of the present invention has no cytotoxicity and skin side effects and can be used safely in cosmetic, pharmaceutical and food compositions.

1 is a graph showing the measurement results of the DPPH free radical scavenging activity of the pink Pleurotus edulis extract of the present invention.
Fig. 2 is a graph showing the results of measurement of tyrosinase inhibitory activity of the pink oyster extract of the present invention. Fig.
FIG. 3 is a graph showing the results of measurement of collagenase inhibitory activity of the pink oyster extract of the present invention.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1: Preparation of pink oyster extract

The pink oyster was cultivated in the mushroom field of the National Institute of Horticultural Science, RDA. The mushroom and mushroom bag were used as the edible parts of the mushroom, and they were dried in a dryer at 50 ° C for more than 8 hours to remove moisture.

The dried pink oyster was mixed with purified water and butylene glycol at a ratio of 1% to the solvent using a mixed solvent of 95: 5 by volume ratio, mixed with a solvent, and extracted at 40 ° C for 15 hours. After the extract was filtered, the filtrate was sterilized at 121 ° C for 15 minutes and used in the experiment.

≪ Test Example 1 > Measurement of total phenol and flavonoid contents of pink oyster extract

 Total phenol content was determined using Folin-Ciocalteau reagent (MP Biomedicals, LLC., France). 50 μl of Folin-Ciocalteau reagent was added to 100 μl of the sample, and the mixture was stabilized for 5 minutes. Then, 300 μl of 20% sodium carbonate was added, and the mixture was further stabilized for 15 minutes. Then, 1.0 ml of distilled water was added thereto and analyzed with a UV / Vis spectrophotometer , Inc., China) was used to measure the absorbance at 725 nm. The measured values were applied to a standard calibration curve prepared using tannic acid (Sigma, St. Louis, Mo., USA) to confirm the total content (· tannic acid equivalent (TA) / mg).

Polyphenol compounds are the most common antioxidants found in most plants and are a generic term for substances with various molecules and structures, including flavonoids, tannins, catechins, and anthocyanins. These polyphenol compounds are characterized by their structural characteristics. They contain phenolic hydroxyl groups that enable them to have various physiological functions such as antioxidant, anti-inflammation and anti-cancer. They can easily bind various proteins and macromolecules to the corresponding functional groups (Park and Lee, 2013; Kuhnau, 1976).

For the determination of flavonoid content, 100 μl of a sample diluted to a constant concentration was added to each reaction vessel, and then 30 μl of 10% ammonium nitrate and 30 μl of 1M potassium acetate were added to the mixture, and then ethyl alcohol (EtOH) diluted to 80% Was added. The reaction solution was allowed to react for 40 minutes and absorbance was measured at a wavelength of 405 nm using a microplate reader (Biochrom EZ Read 400, Cambridge, UK). The measured values were measured by quercetin using quercetin and applied to a standard calibration curve to confirm the total content (· quercetin equivalent (QE) / mL).

content Total phenol content 228.00 ㅁ 4.58 μg · TA ¹⁾ / mL Total flavonoid content 10.83 ㅁ 2.18 μg · QE ²⁾ / mL

The total phenolic content of the extracts contained in the pink oyster extract was 362.36 μg · TA / mL and the total flavonoid content was 10.83 μg · QE / mL. In this case, ¹⁾ TA represents tannic acid equivalent and ²⁾ QE represents quercetin equivalent.

≪ Test Example 2 > Measurement of DPPH free radical scavenging activity of pink oyster extract

The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity for the determination of antioxidative activity of the pink oyster extract was confirmed by electron donating activity against DPPH. As a method of measuring free radical scavenging ability, DPPH can measure antioxidant activity from electron donating ability because it forms an irreversibly stable molecule by receiving electron or hydrogen from antioxidant substance. When it meets a substance having antioxidative activity, free radical (free radical) (Jeon et al., J. Kor. Soc., Food Sic., Nutr. 38 (1)), and the electron donating ability is measured by colorimetry of this color difference. : 1-8, 2009).

100 μl of a 0.2 mM DPPH (Sigma, St. Louis, MO, USA) solution diluted in 100 μl of the sample extract of Example 1 and a light-blocked reagent bottle was added to a 96-well plate, Lt; / RTI > The absorbance (Abs) of the plate was measured at 492 nm using a microplate reader (Biochrom EZ Read 400, Cambridge, UK). The measured values were calculated as EDA (electron donating ability,%) using the following equation. Vitamin C, an antioxidant, was diluted to 10 ppm and 25 ppm as a positive control, and the activity was measured by the above experimental method.

1 is a graph showing the measurement results of the DPPH free radical scavenging activity of the pink Pleurotus edulis extract of the present invention. As shown here, when compared with vitamin C, which is known as a representative antioxidant, the pink oyster extract showed a radical scavenging activity of 78.4%, indicating that it has an excellent antioxidative activity.

<Test Example 3> Tyrosinase inhibitory activity of the pink oyster extract

The whitening effect of the pink oyster extract was evaluated by measuring tyrosinase inhibitory activity as follows.

To 50 μl of the pink oyster extract sample obtained in Example 1 were mixed 80 μl of 1 × sodium phosphate buffer (pH 7.5), 50 μl of 2 mM L-tyrosine and 10 μl of mushroom tyrosinase (1000 U / ml) After the reaction, the absorbance at 490 nm was measured and the tyrosinase inhibitory activity (%) was calculated according to the following formula (2). The results are shown in Fig.

Fig. 2 is a graph showing the results of measurement of tyrosinase inhibitory activity of the pink oyster extract of the present invention. Fig. As shown here, the inhibition rate of tyrosinase was about 30% in the pink oyster. It was confirmed that it has about 3 times higher activity than the 50 ppm vitamin C used as the control group.

<Test Example 4> Measurement of collagenase inhibitory activity of the pink oyster extract

The wrinkle improvement effect of the pink oyster extract was evaluated by measuring collagenase inhibitory activity as follows.

The reaction mixture was added with 4 mM CaCl ₂ in 0.1 M tris-HCl buffer (pH 7.5) to prepare a substrate in which 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg / 0.15 ml of collagenase (0.2 mg / ml) was added to the mixture of 0.25 ml of the solution and 0.1 ml of the sample solution, and the mixture was allowed to stand at room temperature for 20 minutes. Then, 0.5 ml of 6% citric acid was added to stop the reaction, and 1.5 ml of ethyl acetate And the absorbance at 320 nm was measured. Collagenase inhibitory activity was expressed by the absorbance reduction ratio of the sample solution and the non-additive solution.

FIG. 3 is a graph showing the results of measurement of collagenase inhibitory activity of the pink oyster extract of the present invention. As shown here, when the control group was assumed to be 100% active, the pink oyster had a result of about 36%, indicating that it had a collagenase inhibitory activity of about 64%. As a result, it is considered that the pink oyster extract inhibits collagen degradation and may affect skin elasticity and wrinkle improvement.

Claims (3)

A cosmetic composition for improving wrinkles containing an extract of Pleurotus salmoneostramineus as an active ingredient. delete A food composition for improving wrinkles containing an extract of Pleurotus salmoneostramineus as an active ingredient.

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