KR20130123490A - Cosmetic composition comprising maackia amurensis extract for skin whitening - Google Patents
Cosmetic composition comprising maackia amurensis extract for skin whitening Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/044—Suspensions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/046—Aerosols; Foams
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The present invention is Maackia Amurensis ) provides a whitening cosmetic composition comprising an extract as an active ingredient. The cosmetic composition of the present invention inhibits the tyrosinase enzyme, which is an important enzyme for the melanin production mechanism, and suppresses the amount of melanin production, thus showing a remarkable whitening effect. In addition, in the case of the extract of the elm tree which is the active ingredient of the present invention, as well as the above-mentioned whitening cosmetic composition, it can be used as a pharmaceutical composition and a food composition.
Description
The present invention has a skin whitening activity of the elm ( Maackia) amurensis ) extract and a cosmetic composition having a whitening effect comprising the same. More specifically, the present invention relates to an extract of the elm tree having tyrosinase inhibition and melanin biosynthesis inhibitory activity, and a cosmetic composition comprising the same.
The color of human skin, hair, and eyes is determined by melanin, carotene and hemoglobin, and in particular, the most important factor in determining the color of skin is melanin, which is determined by the amount, nature and distribution of melanin. do.
The main function of melanin is to protect the skin from damage by harmful radicals by removing these harmful radicals. Thus, a high level of melanin means that it has an effective countermeasure to protect the skin from physical and chemical toxicants. Factors that promote the production of melanin include not only sunlight (ultraviolet rays) but also hormones such as estrogen, prostaglandine and the like.
Melanin is produced by complex oxidation and condensation reactions in the melanocytes by the sunlight (ultraviolet rays), which is converted into dopa and dopachrome by tyrosinase, an enzyme called tyrosinase. The resulting melanin is delivered to the skin cells and has a circulatory action in which the melanin is lost and disappeared along with epidermal detachment. Since the melanin production mechanism is characterized by the involvement of only one enzyme, tyrosinase, a whitening effect can be expected by inhibiting tyrosinase activity and preventing melanin production.
The method of inhibiting melanin production in whitening cosmetics can be largely divided as follows. The first method is to block and remove ultraviolet light which is a main cause of melanin production, and this method can effectively inhibit melanin production by containing a light scattering agent or a light blocking agent in a cosmetic composition. The second way is to inhibit the production of melanin by inhibiting the synthesis of core carbohydrates required for tyrosinase activity such as glucosamine. The third method is to interfere with the function of tyrosinase involved in melanogenesis, such as enzymatic acid (kojic acid) or arbutin. The fourth method has specific toxicity to melanocytes, such as hydroquinone, which produces melanin, which interferes with cell division. The fifth method is to expect a whitening effect by exerting a strong antioxidative effect while tyrosine, such as vitamin C, undergoes complex oxidation and condensation reaction through dopa and dopachrome.
In recent years, whitening cosmetics, which are considered to be most ideal, firstly block ultraviolet rays, eliminate radicals generated in the body, inhibit the production of inflammatory substances that may be caused by ultraviolet rays, and ultimately inhibit melanin biosynthesis It is known to use complex materials.
However, when a large amount of natural plants are used, it may cause side effects such as irritation to the skin to cause skin pigmentation, and thus it is required to develop a substance and a method capable of maximizing the whitening effect even at a low concentration have.
On the other hand, Korean Patent Registration No. 230130 discloses a whitening cosmetic containing the upper limb extract, Korean Patent Application No. 1999-17810 discloses a whitening cosmetic composition containing the upper limb extract and vitamin C. However, the whitening agents developed to date have a weak whitening effect and have a problem of improving side effects such as skin irritation.
Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.
The present inventors have diligently researched to develop a whitening cosmetic having a very good whitening effect and a low skin irritation compared to a conventional whitening cosmetic. As a result, when using extracts, tyrosinase enzyme, which is an important enzyme for melanin production, is used. The present invention was completed by discovering that it suppresses, suppresses melanin production, and shows a whitening effect.
Therefore, it is an object of the present invention to provide an extract of elm tree showing a whitening effect.
In addition, another object of the present invention to provide a whitening cosmetic composition comprising the extract of the above-mentioned.
Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.
The present invention provides a whitening cosmetic composition.
The present inventors have diligently researched to develop a whitening cosmetic having a very good whitening effect and a low skin irritation compared to a conventional whitening cosmetic. As a result, when using extracts, tyrosinase enzyme, which is an important enzyme for melanin production, is used. It was confirmed that it suppresses and suppresses melanin production and shows a whitening effect.
According to one aspect of the present invention, the present invention exhibits a whitening activity of the elm ( Maackia) amurensis ) extract.
In the present specification, the term 'darm' is native to Korea and is distributed in Japan, China, etc., and may mean plants belonging to the flowering plant door, dicotyledonous plant, rosewood and legumes. The scientific name of the elm is Maackia amurensis to be.
According to a preferred embodiment of the present invention, the elm tree may be selected from the group comprising leaves, stems, berries, roots and outposts of the elm tree, more preferably from the group comprising leaves, stems and fruits. And most preferably may be selected from leaves or stems. The reason why the most preferred extraction part of the tree in this specification is the leaf or the stem is not yet clearly recognized by the public about the efficacy of the tree, but the following examples show that the leaf or stem of the tree is excellent for the whitening effect. Because it proved.
According to a preferred embodiment of the present invention, the extract of the elm tree may be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or water-containing 1-4 carbon atoms. Lower alcohols (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohols with water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) methylene chloride or (h ) 1,3-butylene glycol can be obtained as an extraction solvent. More preferably, it extracts using methanol and ethanol aqueous solution, Most preferably, it can extract using methanol aqueous solution.
According to a preferred embodiment of the present invention, the extract of the elm tree showing the whitening activity may be preferably included in a composition selected from the group comprising a cosmetic composition, a pharmaceutical composition and a food composition, and most preferably used in a cosmetic composition. Can be. Darm tree is known as a plant that has no legal problems in use as a pharmaceutical composition or food composition as a result of KFDA food raw material search ( http://fse.foodnara.go.kr ), and also has excellent effects such as arthritis or boil. In light of this, it is judged that it can be used for the food or pharmaceutical composition which shows a whitening effect.
According to a preferred embodiment of the present invention, the amount of the elm extract is preferably 0.00002-30% by weight, more preferably 0.0001-0.1% by weight, most preferably 0.0025-0.01% by weight relative to the total weight of the whitening cosmetic composition. Can be.
In the composition of the present invention, the most preferable amount of the extract of the elm tree is 0.0025-0.01% by weight, the reason is that when the content of the darnimu extract is less than 0.0025% by weight, the effect of the invention as a skin whitening cosmetics can not be sufficiently obtained And, if it exceeds 0.01% by weight shows a saturation of the effect there is a problem that the efficiency compared to the content decreases.
Therefore, since the content ratio of the extract of Elm tree in the composition of the present invention has a critical meaning, it should be used within the above content ratio.
According to a preferred embodiment of the invention, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It may represent a formulation selected from the group consisting of, and most preferably may be selected from the group comprising softening water, nutrient cream, essence and nourishing cream.
The cosmetic composition of the present invention may include other components in addition to the extract of the elm tree. According to an embodiment of the present invention, the cosmetic composition of the present invention is obtained from the group comprising glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin Further comprising at least one auxiliary component selected from the group consisting of glycerin, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, squalane and sodium citrate, and at least one auxiliary ingredient selected from the group consisting of butylene glycol, citric acid, panthenol and allantoin And most preferably at least one component selected from the group consisting of glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate and allantoin .
Since the cosmetic composition of the present invention is basically applied to the skin, it can be provided with reference to the cosmetic composition of the related art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, But are not limited to, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.
When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.
When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.
When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.
According to a preferred embodiment of the present invention, the composition of the present invention may be a pharmaceutical composition with excellent whitening effect.
When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the formulation and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).
The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans in various routes such as oral or parenteral routes such as oral, rectal or intravenous, muscular, subcutaneous, intra-uterine, Can be administered by injection. Preferably, it is applied by transdermal administration during parenteral administration, more preferably by topical application by application.
The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The dose of the pharmaceutical composition of the present invention can be administered in an amount of 0.1-100 mg / kg on an adult basis once or several times a day in the case of an oral formulation, and in the case of an external preparation, It is preferable to apply it once to 5 times a day in an amount of 3.0 ml and continue for 1 month or longer. However, the dosage is not intended to limit the scope of the present invention.
The pharmaceutical compositions of the present invention may be prepared in unit dose form by formulating with a pharmaceutically acceptable carrier and / or excipient according to methods which can be easily carried out by those skilled in the art. Or may be prepared by incorporation into a multi-dose container. The formulations may be in any form suitable for pharmaceutical preparations including oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols, external preparations such as ointments and creams, suppositories and sterile injectable solutions, , Dispersants, or stabilizers.
According to a preferred embodiment of the present invention, the composition of the present invention may be a food composition excellent in whitening effect.
When the composition of the present invention is prepared with a food composition, it includes not only the active ingredient, but also ingredients normally added during the manufacture of the food, including, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents . Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.
For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, have.
As a result of analyzing cell viability with respect to the active ingredient of the composition of the present invention in the concrete examples of the present invention, it was found that the active ingredient of the composition of the present invention is a harmless substance as a natural substance. Therefore, since the active ingredient of the present invention has little toxicity and side effects, it can be safely used even for long-term use, and can be safely applied to cosmetic, pharmaceutical and food compositions as described above.
The features and advantages of the present invention are summarized as follows:
(a) The present invention provides a whitening cosmetic composition comprising an extract of Maackia amurensis as an active ingredient.
(b) The cosmetic composition of the present invention exhibits a remarkable whitening effect without skin irritation.
(c) The cosmetic composition of the present invention suppresses the tyrosinase enzyme, which is an enzyme important for the melanin production mechanism, and suppresses the amount of melanin production, thereby exhibiting a remarkable whitening effect.
(d) In the case of the extract of the elm tree which is the active ingredient of the present invention, as well as the above-mentioned whitening cosmetic composition, it can be used as a pharmaceutical composition and a food composition.
Figure 1 shows the results of the melanin biosynthesis inhibitory effect of the elm.
Figure 2 shows the results of the melanin biosynthesis inhibitory effect of the elm tree extract.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.
Example
Throughout this specification, "%" used to denote the concentration of a particular substance is intended to include solids / solids (wt / wt), solid / liquid (wt / The liquid / liquid is (vol / vol)%.
Example 1: Preparation of Elm Extract
Example 1-1: Preparation of Methanol Extract
The leaves (1), stem (2), fruit (3), root (4) and starch (5) of the elm tree were each immersed at room temperature for 3 days with MeOH in a ratio of 1:20. This process was repeated three times, followed by filtration through 380 mesh and filtered with 0.45 ㎛ membrane filter. The filtrate was concentrated under reduced pressure at 40 ° C in a distillation apparatus equipped with a cooling condenser, and then evaporated under reduced pressure to obtain a concentrate.
Example 1-2: Elm Hexane Preparation of extract
(1), (2), (3), (4) and (5) of Elm trees were immersed in a 1:20 ratio of hexane, respectively, and immersed at room temperature for 3 days. This process was repeated three times, followed by filtration through 380 mesh and filtered with 0.45 ㎛ membrane filter. The filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and then evaporated under reduced pressure to obtain a concentrate.
Example 1-3: Preparation of Elm Water Extract
(1), (2), (3), (4) and (5) of the elm tree were put in a 1:20 ratio with water, respectively, and extracted at 100 ° C. for 3 hours. After extraction, it was filtered by 380 mesh and filtered by 0.45 mu m membrane filter. The filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and then evaporated under reduced pressure to obtain a concentrate.
Example 1-4: Elm Tree 1,3- Butylene glycol Preparation of extract
(1), (2), (3), (4), and (5) of Elm trees were each immersed at room temperature for 3 days with 1,3-butylene glycol in a 1: 3 ratio. After extraction, the filtrate was filtered through 380 mesh and filtered through a 0.45 μm membrane filter.
Experimental Example One. Mushroom tyrosinase Melanin production inhibitory effect
Experimental Example 1-1: Mushroom tyrosinase Active inhibition
Tyrosinase activity inhibitory activity of the extract of the elm tree was measured by modifying the method such as Yagi, which is measured by the colorimetric method of dopa chrome (DOPA chrome) produced by the action of tyrosinase. That is, the reaction sphere was put into 40 μl of 1.5 mM L-Tyrosine solution (in 0.1 M phosphate buffer (pH 6.5)) and 10 μl of sample solution in 110 μl of 0.1 M phosphate buffer (pH 6.5) in a 96-well plate. 10 µl of tyrosinase (2000 units / ml) was added thereto, followed by reaction at 37 ° C for 10 minutes, and the dopachrome produced in the reaction solution was measured at 490 nm using a microplate counter. In addition, Arbutin was used as a positive control for comparing the test results. The experimental results are shown in Table 1 below.
As a result of experiments with each extract, as shown in Table 1, the tyrosinase activity inhibitory effect was shown as a whole in Example 1-1, but the most excellent leaves and stems were selected and the experiments were carried out by concentration.
Inhibitory effect
As shown in Table 2-3, the leaves and stems of the extract (1) showed an excellent tyrosinase inhibitory effect at a concentration of 50 μg / ml or more, and the effect was 5 times higher than that of the control arbutin. It was.
In the extract (1), it was confirmed that the leaves and stems showing the tyrosinase inhibitory effect were extracted with various other solvents to show the whitening effect.
Inhibitory effect
Inhibitory effect
Inhibitory effect
As a result of the experiment, the following experiment was conducted with the extracts of (1) and (2) of Example 1-1, which were the most effective.
Experimental Example 2: B16 melanoma Melanin production inhibitory effect
Experiment (1) and (2) of Example 1-1 showing the tyrosinase inhibitory effect in Experimental Example 1 were conducted.
Experimental Example 2-1: B16 melanoma Cytotoxicity test for
After culturing B16F1 (mouse melanoma) in 10% FBS-DMEM, the cells were prepared by adjusting the cell concentration to 5 × 10 4 cells / mL. Incubated for 24 hours in a% CO 2 incubator. After incubation for 24 hours, the medium was removed, and 20 μl of Kangjinhyang extract prepared for each concentration and 180 μl of 10% FBS-DMEM were added thereto, and then cultured under the same conditions for 3 days. At this time, the experiment using only 10% FBS-DMEM was used as a control.
After culturing for 3 days, the medium and the solution were removed, 200 μl of 50 μg / ml neutral red (10% FBS-DMEM) was added thereto, and the reaction was carried out for 2 hours in a 37 ° C. incubator. After removing the neutral red solution, add 100 μl of 1% CaCl 2 , 1% formaldehyde (inD.W) solution and stir for 1 minute, remove the solution, and add 1% acetic acid and 50% EtOH (in DW) to 100 After stirring for 15 minutes, the absorbance was measured at 570 nm using a microplate counter.
As in Table 7 and Table 8, the leaves and stems of Example 1-1 did not show cytotoxicity at concentrations of 100 μgml and 25 μgml or less, respectively.
Experimental Example 2-2: B16 melanoma Melanin biosynthesis inhibition experiment
In order to determine the effect of inhibiting the melamine (melanin) biosynthesis in mouse-derived B16F1 melanoma was carried out as follows. After culturing B16F1 (melanoma) in 10% FBS-DMEM, the cells were prepared to adjust the cell concentration to 5.0 × 10 4 cells / ml. Incubated for 24 hours in a% CO 2 incubator. After incubation for 24 hours, after removing the medium, 1.8 mL of the medium prepared with a final concentration of 0.2 uM α-MSH and 200 μl of the sample for each concentration were mixed, and then cultured again under the same conditions for 72 hours. In this case, Blank was added only to the medium without the addition of α-MSH, and the same concentration of arbutin was used as the positive control. After incubation for 72 hours, the plate was washed twice with 1 × PBS (-) in order to measure the amount of melanin in the solution. Then, 200 μl of Trypsine-EDTA solution was added to the cells. The supernatant was removed. The separated pellets were washed twice with 1 × PBS (−) and dried at 70 ° C. for 1 hour. After dissolving 400 μl of 10% DMSO (in 1 M NaOH), 200 μl of the sample was dispensed into a 96-well plate, and the absorbance was measured at 490 nm using a microplate counter.
As shown in FIGS. 1 and 2, the extract of the elm tree shows a good melanin biosynthesis inhibitory effect. However, the effect was not shown in the leaves, only the stem was effective, in particular, at 25 ㎍ml concentration was confirmed that the inhibitory effect of melanin biosynthesis higher than 500 ㎍ml Arbutin control.
Prescription example 1: Preparation of Softening Cosmetics Containing Elm Extract
Example 1-1 (2) An example of the formulation of the flexible longevity in the cosmetic containing the extract is as follows.
Sodium hyaluronate was prepared as a 1% solution by dispersing at 3000 rpm propeller mixer in purified water. The raw materials 1-8 were homogenized at 500 rpm in a propeller mixer and completely dissolved by heating at 75 ° C., and then cooled to room temperature. After completely dissolving the raw materials 9-11 in a separate dissolution tank, the mixture was stirred and mixed. Example (1) -stem was added to this, and the mixture was sufficiently stirred and mixed, thereby preparing a flexible cosmetics containing Example (1) -stem.
Prescription example 2: Preparation of Nutrients Containing Elm Extract
Example 1-1 (2) A prescription example of nutritional longevity in cosmetics containing the extract is as follows.
Carbomer was prepared as a 2% solution by dispersing in propeller mixer 4000 rpm in purified water. The raw material 1-6 was thrown into the water phase dissolution tank, and it stirred at 2000 rpm and disperse | distributed, and heated to 75 degreeC. The raw material 7-13 was thrown into the oil phase dissolution tank, and it melt | dissolved by heating to 75 degreeC. The oil phase dissolved in the aqueous phase dissolution tank was added to emulsify (3000 rpm / 5 minutes), and then cooled to room temperature. Example (1) -stem was added thereto and stirred and mixed sufficiently to prepare Example (1) -nutrition lotion containing the stems.
Prescription example 3: Preparation of Essence Containing Elm Extract
Example 1-1 (2) An example of the prescription of the essence in the cosmetic composition containing the extract is as follows.
Sodium hyaluronate and hydroxyethyl cellulose were each dispersed in purified water at 2000 rpm with a propeller mixer to prepare a 1% solution. Carbomer was prepared as a 2% solution by dispersing in propeller mixer 4000 rpm in purified water. The raw material 1-12 was thrown into the aqueous phase dissolution tank, and it stirred at 2000 rpm and disperse | distributed, and heated to 75 degreeC. The warmed water phase was cooled to room temperature. The raw materials 13 to 15 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. Example (1) -stem was added thereto and sufficiently stirred and mixed to prepare an essence containing Example 1-1.
Prescription example 4: Preparation of Essence Containing Elm Extract
Example 1-1 (2) An example of the prescription of the nourishing cream in the cosmetic composition containing the extract is as follows.
The raw material 1-8 was thrown into the water melting tank, it stirred at 2000 rpm, and it disperse | distributed, and it heated up to 75 degreeC. The raw material 9-16 was thrown into the oil phase dissolution tank, and it melt | dissolved by heating to 80 degreeC. The oil phase dissolved in the aqueous phase dissolution tank was added to emulsify (3000 rpm / 10 minutes), and then cooled to room temperature. Example 1-1 was added thereto and sufficiently stirred and mixed to prepare a nutrition cream containing Example (1) -stem.
Experimental Example 3: skin whitening effect test
In order to confirm that the whitening effect shown in the in-vitro experiment actually helps human skin whitening, a human application test was performed on the cream containing Example 1-1 (2). The skin whitening test using the Roboskin analyser equipment evaluated the effect of pigmentation and improvement of skin tone (brightness) periodically while applying a sample or a product to the subject's face area. The test was also performed in the same manner as the skin elasticity improvement test, and therefore, 10 subjects (5 control and 5 test subjects) were conducted, and all of them were women with an average age of 34 years. The average value was obtained by measuring five times before, after 1, 2, 4 and 6 weeks of use. All tests were conducted in a constant temperature and humidity room with 20-24 ℃ and 45-55% humidity. It was measured after adjusting for at least 1 hour in a humidity room.
As a result of measuring the skin whitening effect of Formulation Example 4, in the case of Formulation Example 4, pigmentation (large and small) also tended to decrease from the 2nd week, and the brightness of the skin was also brightened, which helped skin whitening. I could see that.
Claims (7)
The extract of the elm is selected from the group comprising leaves, stems, fruits, roots and outposts of the elm.
Wherein the extracting solvent of the extract is selected from the group consisting of water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, nucleic acid, ethanol, butylene glycol, chloroform and mixtures thereof.
The amount of the elm extract is a whitening cosmetic composition, characterized in that 0.00002-30% by weight based on the total weight of the cosmetic composition.
The cosmetic composition comprises a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Whitening cosmetic composition characterized by having.
Extract is characterized in that the use of the extract is used in addition to the whitening activity of the cosmetic composition for use in whitening activity of the pharmaceutical composition or food composition.
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KR1020120046637A KR20130123490A (en) | 2012-05-03 | 2012-05-03 | Cosmetic composition comprising maackia amurensis extract for skin whitening |
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KR1020120046637A KR20130123490A (en) | 2012-05-03 | 2012-05-03 | Cosmetic composition comprising maackia amurensis extract for skin whitening |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20150064835A (en) | 2013-12-04 | 2015-06-12 | 코웨이 주식회사 | A cosmetic composition for skin whitening comprising the extract of eucalyptus occidentalis endl as active ingredient |
KR20150065028A (en) | 2013-12-04 | 2015-06-12 | 코웨이 주식회사 | A cosmetic composition for skin whitening comprising the extract of derris dalbergioides baker as active ingredient |
KR101534557B1 (en) * | 2014-04-15 | 2015-07-09 | 한국콜마주식회사 | Skin-whitening composition containing Maackiasin thereof as an active ingredient |
-
2012
- 2012-05-03 KR KR1020120046637A patent/KR20130123490A/en not_active Application Discontinuation
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20150064835A (en) | 2013-12-04 | 2015-06-12 | 코웨이 주식회사 | A cosmetic composition for skin whitening comprising the extract of eucalyptus occidentalis endl as active ingredient |
KR20150065028A (en) | 2013-12-04 | 2015-06-12 | 코웨이 주식회사 | A cosmetic composition for skin whitening comprising the extract of derris dalbergioides baker as active ingredient |
KR101534557B1 (en) * | 2014-04-15 | 2015-07-09 | 한국콜마주식회사 | Skin-whitening composition containing Maackiasin thereof as an active ingredient |
WO2015160030A1 (en) * | 2014-04-15 | 2015-10-22 | 한국콜마주식회사 | Maackiasin having skin whitening activity and use thereof |
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