KR101003470B1 - Cosmetic composition for antioxidation containing the extract of ancient rice - Google Patents

Abstract

PURPOSE: A cosmetic composition for antioxidation containing ancient extract is provided to ensure whitening, anti-aging, skin cell protection and anti-inflammation. CONSTITUTION: A cosmetic composition for protecting skin cells contains ancient rice extract as an active ingredient. The cosmetic composition for protecting skin cells contains red rice extract as an active ingredient, which is isolated using 5% HCl/MeOH. The extract is isolated using methanol or ethanol. The red rice contains cyaniding-3-glucoside.

Description

Cosmetic composition for antioxidation containing ancient rice extract {Cosmetic composition for antioxidation containing the extract of ancient rice}

The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition for antioxidants containing ancient rice extract.

Skin is a very important tissue that protects the human body while having direct contact with the external environment and has biochemical and physical functions. In recent years, there is a high risk of damage to the skin caused by exposure to ultraviolet rays due to the destruction of the ozone layer along with the serious air pollution problem. Therefore, the selection of functional products related to cosmetics or cosmetics is concerned with side effects and prevents skin oxidation. Is a very important issue in the world.

The causes of aging include UV rays, mental stress, and the external environment. In this case, the most important role is reactive oxygen species, which in turn promotes adult disease and aging by damaging cells and tissues. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

Skin aging by pigmentation is affected by pigments such as melanin, melazoids, carotene, hemoglobin and the like. The most important of these is melanin, which has no special maximum absorption wavelength and absorbs light in all areas. In addition, the function of removing active oxygen species is excellent, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself free radicals. It may also be indicated.

The biosynthesis of melanin begins with the conversion of tyrosine, an amino acid, from melanocytes of melanocytes to oxidization by tyrosinase to dihydroxy phenylalanine. After a series of oxidation processes, it is formed into polymers of brown (pheomelanin) and black (eumelanin), and this biosynthesis process is carried out in a special type of brown intracellular organelles called melanosomes. Melanosomes, including melanin granules, migrate from the periphery of the nucleus to the end of the dendritic projections and into the cytoplasm by the phagocytosis of keratinocytes, which accumulate around the nucleus of keratinocytes.

Meanwhile, ascorbic acid (JP-A 4-9320), hydroquinone (JP-A 6-192062), koji-san (JP-A 56-7710), arbutin (JP-A 4-93150) and some extracts It has been used as a whitening cosmetic because it has a tyrosinase inhibitory activity, but its use is limited due to poor stability in cosmetic formulations, discoloration, odor occurrence, efficacy at the biological level, unclear effect and safety issues, etc. There is a situation.

Cosmetics should be free of side effects, human-friendly, and safe to use on specific constitutions, atopic or sensitive skin. The plant natural products are not only excellent in safety, but also have various components beneficial to the human body, and thus are preferred as ingredients that provide moisturizing, whitening, and anti-aging effects. Thus, conventionally, there have been efforts to find extracts that provide excellent safety and provide various functionalities from herbals, vegetables, fruits, flowers, etc., which have a function of suppressing skin aging and whitening.

On the other hand, as for natural extracts, Korean Patent Publication No. 2003-0064059 (bark extract, silk tree extract), Korean Patent Publication No. 2003-0064059 (rose petal extract) and the like have been disclosed, Korean Patent Publication No. 2003-00640 0064059 has only anti-aging function, and Korean Patent Laid-Open Publication No. 2003-0064059 has only skin whitening function, so one extract does not simultaneously exhibit various effects such as antioxidant, whitening effect, wrinkle improvement effect, and cellular protection effect. Was limited.

Thus, the inventors of the present invention conducted a thorough study based on various plant extracts, in particular, the ancient rice extract, found that not only has an excellent antioxidant effect, but also has various effects such as whitening effect, wrinkle improvement effect, skin protection effect, anti-inflammatory effect. And to complete the present invention.

It is an object of the present invention to provide a cosmetic composition which is harmless to the human body and is excellent in stability as well as excellent in antioxidant effect.

In addition, the present invention is not only excellent in the antioxidant effect, but also has a number of effects, such as whitening effect, wrinkle improvement effect, skin cell protection effect and anti-inflammatory effect at the same time, and also aims to provide a cosmetic composition excellent in each effect very excellent. .

In order to achieve the above object, the present invention provides a cosmetic composition for antioxidant, characterized in that it contains an ancient rice extract as an active ingredient.

In another aspect, the present invention provides a cosmetic composition for whitening, characterized in that it contains red pepper extract as an active ingredient.

In addition, the present invention provides an anti-aging cosmetic composition, characterized in that it contains red pepper extract as an active ingredient.

In another aspect, the present invention provides a cosmetic composition for protecting skin cells, characterized in that it contains red pepper extract as an active ingredient.

In addition, in the present invention, the extraction solvent of the ancient rice extract preferably includes methanol or ethanol.

In addition, in the present invention, it is preferable that the extraction solvent of the red Tommy extract contains methanol or ethanol.

Hereinafter, the means for solving the problems of the present invention will be described in detail.

Ancient rice used in the present invention is a wild rice seed rice before the current varieties improved rice has a dormant, degranular properties, refers to rice that is not artificially grown and grown out of reach of humans like weeds, and after frost It is characterized by being cultivated. There are red clay, black clay, and green clay in the ancient rice, and especially in the case of red clay, the polyphenol content is 200 times higher than that of white rice.

Red tomy is reddish brown brown rice, which refers to rice containing tannin, a pigment of red color, in the skin or seed skin. Red rice is similar to white rice and contains a lot of protein, vitamins and minerals. Especially, it contains tannin, a kind of polyphenol, which is known to have antibacterial and antioxidant activity. In addition, red meat is known to have the effect of lowering the body's sugar and cholesterol levels.

Black rice is black brown rice, which is rich in protein, vitamin B1, B2, niacin, vitamin E, iron, calcium, magnesium, etc., compared with modern white rice, and especially anthocyanin contained in the skin It is known to have a protective antioxidant activity. It is a well-known rice that has been known by Chinese emperors in China.

Noktomi is a traditional indigenous seed introduced by soft rice in Dongbogam. It is more sticky and sweeter than ordinary glutinous rice, and contains abundant chromophyll, zinc, magnesium, and fiber. It is known.

As a result of earnestly studying the components of red Tommy, the inventors of the present invention found that red Tommy contained a large amount of cyanidin-3-glucoside. Cyanidin-3-glucoside (Cyanidin-3-glucoside) is an anthocyanin-based pigment component, a component known to be contained in the black soil. However, the inventors of the present invention have found that cyanidin-3-glucoside is contained in a content similar to red clay and black clay.

The ancient rice extract used in the present invention may be obtained through various methods known in the art, and is preferably extracted using an extraction solvent. Preferred examples of such extractants are water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol, butanol, etc.), acetone, ethyl acetate, chloroform, 1,3-butylene glycol or two of them. There are mixtures of species or more, more preferably methanol or ethanol, and a mixed solution of methanol and hydrochloric acid may be used. As the extraction solvent, the above-mentioned ones are particularly suitable but not necessarily limited thereto, and the ancient rice extract may be prepared using other solvents similar in physical and chemical properties to the above-mentioned solvents.

In addition, the ancient rice extract of the present invention includes not only the extract by the aforementioned extraction solvent, but also extract extracted by other methods. Separation using, for example, an ultrafiltration membrane having a constant molecular weight cut-off value; Fractions obtained through various purification methods additionally carried out, such as by chromatography prepared for separation according to various chromatography, for example, size, charge, hydrophobicity or affinity, are also included in the ancient rice extract of the present invention.

In addition, in the present invention, the ancient rice which is a material for extraction may be used by drying it or may be used as it is, and preferably used by drying. As a drying method, natural drying or a hot air drying method can be used. In addition, the ancient rice may be used by grinding in order to facilitate extraction.

In addition, the ancient rice extract of the present invention may be used in a liquid state, but may also be prepared and used in a powder state by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

On the other hand, the cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art. For example, a base cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O) type, oil-in-water and water-in-oil makeup base, foundation, skin cover, Lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencil formulations of the cosmetic composition and body lotion, body oil, body essence, body pack, such as any one can be selected from the body formulation. .

The present invention relates to a cosmetic composition containing the ancient rice extract as an active ingredient, the composition of the present invention is not only very excellent in the antioxidant effect, but also excellent in the whitening effect, wrinkle improvement effect, protection of damaged cells and anti-inflammatory effect. Therefore, the cosmetic composition of the present invention shows a very excellent comprehensive skin improvement effect.

As described above, the cosmetic composition of the present invention is not only excellent in the antioxidant effect by containing the ancient rice extract, but also excellent in the whitening effect, wrinkle improvement effect, protective effect of the damaged cells and anti-inflammatory effect. The cosmetic composition of the present invention exhibits a very excellent comprehensive skin improvement effect.

1 is a view showing a standard curve of the standard product.
2 is a diagram showing a chromatogram of a standard product.
3 is a diagram showing a chromatogram for red soil.
4 is a diagram showing a chromatogram for black soil.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of equivalent technical ideas.

Production Example  One: Antiquity  Extract manufacturer

The extract of ancient rice was prepared by adding methanol, 5% HCl / MeoH, 95% ethanol, 70% ethanol as an extraction solvent and extracting at room temperature for 5 days with an extractor. The extract was filtered with a 400 mesh filter cloth and filtered again with a 0.45 filter to prepare red tomy extracts 1 to 4, black tome extracts, and noctotomy extracts as shown in Table 1 below. In addition, white rice extract was prepared in the same manner as a comparative preparation.

extract Extraction solvent White rice extract 70% EtOH Red Tommy Extract 1 MeOH Red Tommy Extract 2 5% HCl / MeOH Red Tommy Extract 3 95% EtOH Red Tommy Extract 4 70% EtOH Black Tome Extract 70% EtOH Nocturne extract 70% EtOH

Experimental Example  One: Antiquity  Antioxidant Effect of Extracts

In order to measure the antioxidant effect of the ancient rice extract, DPPH (radical) scavenging effect of the ancient rice extract was measured using the reducibility of DPPH (1,1-diphenyl-2-picrylhydrazyl).

Experimental method was carried out in the following manner.

1) To 1 mL of 0.1mM DPPH solution dissolved in methanol, red mule extract 1 to 4, black tome extract and noctomy extract obtained in Preparation Example 1 were diluted at each concentration, and 500 µl each were mixed and mixed.

2) After reacting for 10 minutes at room temperature, the absorbance was measured at 565 nm.

sample IC ₅₀ (μg / mL) White Rice Extract 1,190.0 Red Tommy Extract 1 279.8 Red Tommy Extract 2 128.6 Red Tommy Extract 3 88.2 Red Tommy Extract 4 30 Black Tome Extract 50 Nocturne extract 960

As shown in Table 2, it was confirmed that the high DPPH radical scavenging ability in each red and black extract. In particular, the red antioxidant extract 4 showed the highest antioxidant capacity, from which it can be seen that the extraction of the antioxidant component in the case of 70% ethanol extraction solvent is the easiest.

Experimental Example  2: Red Tommy  Determination of Whitening Effect of Extracts

In order to measure the whitening effect of red Tommy extract, the melanoma cell line was used to measure the extent to which red Tommy extract inhibits the amount of melanin that is finally produced by the melanoma cell line. .

The amount of melanin (mg) contained in protein (g) was determined by quantifying melanin and protein quantification of red Tommy extract, and based on this, the melanogenesis inhibition rate (%) was determined. Could save.

As the experimental cell line, 'B16F10 Melanoma cell line' was used, and arbutin was used as a control.

Experimental method was carried out in the following manner.

1) B16F10 cells containing 5% carbon dioxide at 37 ° C. using Dulbecco's modified Eagle's medium (DMEM) containing penicillin (100 U / mL), streptomycin (100 μg / mL) and 10% Fetal bovine serum (FBS) The culture was carried out in an incubator.

2) Inoculated at 1 × 10 ⁵ cells / mL per well of a 6-well plate and incubated for 24 hours.

3) After incubation, the medium was removed and each red Tommy extract 1 obtained in Preparation Example 1 was diluted to a concentration of 50 and 100 μg / mL using a medium containing serum (α-MSH 50 nM). After the addition, further incubation for 48 hours.

4) The cells were washed with PBS and treated with 500 μl of Trypsin-EDTA to remove the cells, and 500 μL of PBS was added to recover the cells.

5) The recovered cells were centrifuged at 12,000 rpm for 10 minutes at 4 ° C to remove the supernatant, and the pellets were dried at 65 ° C for about 1 hour.

6) 120 μL of an aqueous 1 M NaOH solution was added and reacted at 65 ° C. for 1 hour to obtain melanin in the cells.

7) After the lysate of 6) was put into each 96-well plate (90 μL), the absorbance was measured at 490 nm with an ELISA reader to measure the amount of melanin.

8) Using the lysate of 6) quantify the protein by the BCA method to determine the amount of melanin (melanin) for the amount of protein, using a synthetic melanin (Synthetic melanin) to obtain a standard curve of the melanin (melanin) The amount was quantified and shown in Table 3 below.

sample Concentration (㎍ / mL) Melanogenesis Inhibition rate (%) Arbutin 50 4.31 100 14.33 Red Tommy Extract 1 50 7.49 100 29.25

According to Table 3, in the case of red Tommy extract 1 having a concentration of 100 μg / mL, the melanin production inhibitory activity was about 29%, which was 2 times higher than that of the arbutin positive control group. .

Experimental Example  3: Red Tommy  Determination of Whitening Effect of Extracts

The whitening effect of red tooth extract was determined by evaluating relative enzyme activity by measuring the amount of dopachrome produced by tyrosinase using a substrate by absorbance.

Enzyme was used as mushroom tyrosinase (SIGMA), and koji acid was used as a control.

Experimental method was carried out in the following manner.

1) 1.5mM tyrosine (in 1 N HCl), 100U / mL Tyrosinase (0.1O) and 0.1M sodium phosphate buffer (Sodium Phosphate Buffer, pH 6.5) was prepared.

2) Each red tomi extract 1 to 4 obtained in Preparation Example 1 was prepared by diluting to 50 µg / mL using sodium phosphate buffer (final sample concentration).

3) Put 100μL of 1.5mM tyrosine prepared in a 96-well plate, 20μL of sample, 3 wells per concentration, and add 80μL of 100U / mL tyrosinase prepared last. Mix well, and react for 15 minutes in a 37 ℃ incubator (incubator).

4) After the reaction, the absorbance value was measured at 490 nm with an ELISA reader, and is shown in Table 4.

sample Concentration (㎍ / mL) Inhibitory activity of tyrosinase
(% of control) Red Tommy Extract 1 50 9.38 Red Tommy Extract 2 50 27.73 Red Tommy Extract 3 50 6.03 Red Tommy Extract 4 50 3.35

According to Table 4, it was confirmed that the tyrosinase inhibition rate (Tyrosinase inhibition rate) is the highest tyrosinase activity of about 27% in the case of red clay extract 2 having a concentration of 50μg / mL.

Experimental Example  4 : Red Tommy  Cell damage of extract Protection  evaluation

Free radicals inhibit the immune function of the skin, induce inflammation and cause various skin diseases such as decreased elasticity, wrinkles, blemishes, and freckles, which in turn accelerate skin aging, thus protecting cells from free radicals. The effect was measured to evaluate the recovery of damaged skin cells in the following manner.

1) After inoculating human-derived keratinocytes (HaCaT cells) at the bottom of the culture dish, DMEM containing penicillin (100 U / mL), streptomycin (100 µg / mL), 10% FBS (Fetal Bovin Serum) (Dulbecco's Modified Eagle's Medium) medium was added and cultured in an incubator containing 5% carbon dioxide at 37 ℃.

2) HaCaT cells were inoculated at a concentration of 1 × 10 ⁵ cells / mL in 96-well plates at 100 μl and incubated for 24 hours.

3) After incubation, all of the medium was removed, and the sample was diluted to 10, 50, 100 µg / mL using a medium without serum, and treated with 100 µl per well three times, followed by an additional 24 hours. Incubated.

4) The cultured cells were treated with 200 μM of hydrogen peroxide (H ₂ O ₂ ) as a cell damaging material, and cultured in an incubator containing 5% carbon dioxide at 37 ° C. for 4 hours.

5) After 4 hours, the medium was removed, and 100 µl of fresh serum-free medium was inoculated, and 20 µl of MTT reagent dissolved at 5 mg / mL was added using PBS, followed by incubation for 4 hours.

6) After removing all of the medium containing the MTT reagent and the sample, 100 μl of isopropanol was added to each well and stirred for 15 to 20 minutes, and the absorbance value was measured at 570 nm using an ELISA reader. On the other hand, cell damage protection was measured by the following method.

Cell damage protection function = cell viability during sample handling (%) when H ₂ O ₂ treated cell survival rate (%)

sample Concentration (/ ml) Cell damage protection (%) Quercetin 10 - 20 6.48 50 12.79 Red Tommy Extract 2
10 31.98 50 31.68 100 31.43

As a result of comparing and evaluating quercetin (Quercetin), which is known as a cell protective substance, as a positive control group, it was confirmed that the cell damage protection at the same concentration of 50 ㎍ / ml showed more than twice the effect of the red control extract 2 compared to the positive control group. there was.

Experimental Example  5: Red Tommy  Determination of Anti-inflammatory Effects of Extracts

Excessive generation of NO (Nitric Oxide), a reactive nitric species (RNS), induces an inflammatory response. To this end, the cells are stimulated with lipopoly-saccharide (LPS) to induce the production of NO. The effect was measured by the 'Griess reagent' method.

The experimental cell line was used as 'Raw 264.7 cell line', and the control group was 50 μM of NG-methyl-L-arginine acetate salt (L-NMMA) and NaNO _2. (NO standard) 50 μM was used.

Experimental method was carried out in the same manner as the following method (prior to the experiment was carried out by checking the cytotoxicity by using the MTT assay method, and then select a non-toxic concentration).

1) After inoculating a mouse-derived macrophage (Raw264.7) to the bottom of the culture dish, DMEM containing penicillin (100U / mL), streptomycin (100μg / mL), 10% FBS (Fetal bovine serum) Dulbecco's modified Eagle's medium) was added and cultured in an incubator containing 5% carbon dioxide at 37 ° C.

2) Raw264.7 cells were seeded in 96-well plates at a concentration of ⁵ × 10 ⁵ cells / mL and incubated for 24 hours.

3) After incubation, the medium was removed and each red Tommy extract 3, 4 obtained in Preparation Example 1 was diluted to 50 μg / mL using a medium containing serum (LPS 100 ng / mL), and then each well. After the addition, the cells were further incubated for 24 hours.

4) The medium was recovered and centrifuged at 12,000 rpm for 10 minutes at 4 ° C. After that, only medium was obtained and 50 μL was added to a 96-well plate and reacted with the same amount of Griss reagent for 10 minutes. I was.

5) After the reaction, the absorbance value was measured at 540 nm using an ELISA reader, and the results are shown in Table 6 below.

sample Concentration (㎍ / mL) NO production inhibition (%) Red Tommy Extract 3 50 3.44 Red Tommy Extract 4 50 16.28

The production inhibition of red Tommy extracts against NO production induced by artificial stimulation (LPS) was measured, and the red Tommy extract 4 was found to have NO production inhibition rate of about 16%.

Experimental Example  6: Red Tommy  Evaluation of Wrinkle Inhibitory Effect of Extracts (Cells Proliferative capacity  evaluation)

Wrinkle inhibition efficacy evaluation was carried out to the red Tommy extract obtained in Preparation Example 2.

Experimental methods were carried out in the same manner as the following method, and the concentration was determined to have no toxic effect on the cells by MTT assay before performing the experiment.

1) After inoculating human-derived fibroblasts (CCD-986sk) at the bottom of the culture dish, IMDM medium containing penicillin (100 U / mL), streptomycin (100 μg / mL) and 10% FBS (fetal bovine serum) Incubated at 37 ℃ incubator containing 5% carbon dioxide.

2) CCD-986sk cells were diluted to a concentration of 1 × 10 ⁵ cells / mL in a 96 well plate and inoculated with 100 μl and incubated for 24 hours.

3) After incubation, all of the medium was removed and 90 μl of medium without serum was added to each well.

4) The red Tommy extract of Preparation Example 2 diluted with a medium containing no serum so as to have a final concentration of 50 and 200 μg / ml was treated to 10 μl of wells 3).

5) After 24 hours of incubation, 20 μl of MTT reagent dissolved at a concentration of 5 mg / mL was added using PBS and incubated for 4 hours.

6) Remove all medium containing MTT reagent and sample, add 100µl of isopropanol acid (0.04N HCl in iso-propanol) to each well, stir for 30 minutes, and 570nm using ELISA reader. Absorbance value was measured at.

sample Concentration (ug / ml) Cell proliferation (%) Red Tommy Extract 1 50 16.84 Red Tommy Extract 2 50 16.18 Red Tommy Extract 3 200 31.43 Red Tommy Extract 4 200 14.58

As shown in Table 7, the cell proliferation was confirmed in each red Tommy extract, from which the red Tommy extract was determined to have the effect of suppressing wrinkles caused by skin aging.

Experimental Example  7: Red Tommy  And Black soil  undergarment Cyanidin -3-glucoside ( Cyanidin -3-glucoside) content determination

Cyanidin-3-glucoside content in red and black soil was measured by the following method.

The standard solution was used as a standard solution by diluting the Kuromanin chloride standard in ethanol at a concentration of 100 ppm, 50 ppm, and 10 ppm.

Samples were black and red, and HPLC (high performance liquid chromatography) was used, and the conditions were as follows.

The column is' Agilent Eclipse plus C18 (5㎛, 4.6mm id x 250mm), the detector is' UV / VIS Spectrophotometer (280nm) ', flow rate is 1.0mL / min, column The temperature was 40 ° C., the injection volume was 10 μl, and the mobile phase was acetylnitrile: 5% formic acid (MeOH): 5% formic acid (40: 55: 5).

First, the area values according to the standard concentration are shown in Table 8, the standard curve is shown in FIG. 1, and the chromatogram is shown in FIG. 2.

density Area 10 ppm 237304 50 ppm 1205910 100 ppm 2373535

Next, after passing red and black soil through HPLC, the chromatograms are shown in Figs. 3 and 4, based on this, cyanidin-3-glucoside in red and black soil. The content is shown in Table 9.

sample density(%) Red Tommy 0.2673 Black soil 0.2578

In general, cyanidin-3-glucoside is known to be found mainly in black soil, but according to FIG. 3 and Table 9, cyanidin-3-glucoside Was found more in red soil than in black soil. Therefore, it was confirmed that cyanidin-3-glucoside was present in a considerable amount of red Tommy used in the present invention.

Experimental Example  8: Red Tommy  And Black soil  Total polyphenols in Total polyphenol Content measurement

The total polyphenol content of red and black soil was analyzed by Folin-Denis method, and the experimental method was performed as follows.

Red, black and white rice were used as samples, and 0.5 mL of 30% MeOH and 0.5 mL of phenolic reagent of Folin & Ciocalteau were added to each sample, mixed, and 0.5 mL of saturated Na ₂ CO ₃ solution was added and left in the cow for 1 hour. Afterwards, the absorbance was measured at 700 nm with a UV / Vis spectrophotometer. In addition, the total polyphenol content was converted using a standard curve prepared by measuring the same method using gallic acid as a standard.

sample Total Polyphenol Content (mg / 100g) Red Tommy 7145.24 ± 54.56 Black soil 3145.24 ± 54.53 White rice 34.76 ± 2.06

According to the existing research results, the polyphenol component of the plant component is known to exhibit the effects of antioxidant, anti-inflammatory, anti-cancer, anti-aging, etc. According to Table 10, the total polyphenol content in red soil is 7145mg / 100g, black Two times more than Tommy and 200 times more than white rice was found to contain a significant amount.

Claims (6)

delete delete delete Cosmetic composition for skin cell protection, characterized in that it contains red tomy extract extracted with 5% HCl / MeOH as an extractant as an active ingredient, and protects skin cells from active oxygen delete delete

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