KR101787406B1 - Cosmetic composition comprising bromelain, butcherbroom, grape seeds, arnica and lemon balm - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising as an active ingredient a mixture containing bromelain, budurobrom, grape seed, arnica and lemon balm as an active ingredient. The cosmetic composition is excellent in skin astringency, antioxidant or swelling-reducing effect.

Description

[0002] Cosmetic compositions comprising bromelain, butcherbroom, grape seeds, arnica and lemon balm as active ingredients,

The present invention relates to a cosmetic composition comprising as an active ingredient a mixture containing bromelain, budurobrom, grape seed, arnica and lemon balm as an active ingredient. The cosmetic composition is excellent in skin astringency, antioxidant or swelling-reducing effect.

As the skin ages, the skin becomes dry, wrinkles and loses its elasticity. Free radicals and reactive oxygen species are the most important treatments in skin aging research.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a toxic substance produced by cells by physiological action such as respiration and is constantly produced and extinguished, and is present in 3 to 5% of normal conditions . These active oxygen species are present as free radicals such as superoxide radicals (O ₂ ^- ), hydroxyl radicals (HO ⁺ ), or as free radicals such as hydrogen peroxide (H ₂ O ₂ ) (Singlet radical, ¹ O ₂ ). Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. These reactive oxygen species attack biomolecules and damage cells and tissues, and it is reported that they cause various kinds of diseases related to aging and diseases of various adult diseases. Studies on antioxidants capable of inhibiting the oxidative action by these active oxygen species have been actively carried out, and research and development of natural substances and substances having excellent antioxidative effects have been attracting attention as aging prevention materials.

One of the functions that prevents skin aging and maintains healthy skin is convergence. The principle of convergence is the phenomenon that the skin contracts due to the formation of crosslinks by binding with the polymer flavonoids of the skin protein. Convergence means basically wrinkled or funky. Convergent agent has a function to shrink skin and mucosal blood vessels, and has an effect of inhibiting secretion of mucus by interrupting cell gap and lymphatic gap. In addition, since astringent agent has a property of binding to protein, it can generally judge degree of convergence effect according to degree of binding of hemoglobin protein with extract. Therefore, the convergence effect is to give the skin a sense of resilience and flexibility by contracting excessively opened pores and pores.

Edema is a phenomenon of excessive accumulation of water. There are more than 70% body fluids in our body, two thirds of which are intracellular fluid, and one third is extracellular fluid. 1/4 of the extracellular fluid is blood, and the remaining 3/4 is the interstitial fluid. When the amount of interstitial fluid increases, the body becomes swollen. Among them, the swelling of the facial surface deforms the impression and gives a cosmetically unfavorable effect.

Conventionally, as a method for improving the swelling of the face, a method of giving a cooling sensation to the face by using a gel type cream formulation containing a large amount of water and alcohol, a method of applying a hydrogel type patch, And a method of imparting a tension to the skin with a temporary cooling sensation due to evaporation. However, the feeling of cooling was often temporary, making it difficult to improve swelling fundamentally.

Accordingly, the present invention provides a cosmetic composition which can exhibit skin convergence, antioxidant and swelling-reducing effects using natural extracts.

It is an object of the present invention to provide a cosmetic composition which is excellent in antioxidation, skin convergence and swelling reduction effects, including bromelain, budurobrom, grape seed, arnica and lemon balm.

In order to achieve the above object,

The present invention provides a cosmetic composition comprising, as an active ingredient, a mixture comprising bromelain, budule broth, grape seed, arnica and lemon balm.

The cosmetic composition comprising the mixture of the present invention containing bromelain, budurobrom, grape seed, arnica and lemon balm as an active ingredient shows the effects of antioxidation, skin convergence and swelling reduction.

1 is a graph showing the antioxidative effect of the cosmetic composition of the present invention.
Fig. 2 is a graph showing the skin convergence effect of the cosmetic composition of the present invention.
FIG. 3 is a graph showing swelling improvement rate according to the water content in the experimental example.
4 is a graph showing swelling improvement rate according to the volume change in the experimental example.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition comprising as an active ingredient a mixture comprising bromelain, budule broth, grape seed, arnica and lemon balm.

The cosmetic composition of the present invention can exhibit the effects of antioxidation, skin convergence and swelling reduction by including the above-mentioned mixture.

Bromelain, a raw material contained in the cosmetic composition according to the present invention, is a mixture of various compounds of plant protease and other unidentified ingredients extracted from the fruit and stem of pineapple (Ananas comosus). It is a substance that is widely used for the decomposition of muscle fiber and anti-cancer, anti-platelet, respiratory inflammation, indigestion, rheumatoid arthritis and mastitis treatment. It is mainly used as an anti-inflammatory agent and anti-edema agent, and is known to be effective for treating inflammation of soft tissues, traumatic event, and inflammation after post-surgical tissue reaction. It is known that the inflammation treatment is caused by improving the nutritional supply and enhancing the immune function of the inflammatory site such as decomposition of the inflamed area fibrin layer, anti-edema, pain relief and improvement of blood circulation.

The butcherbroom, a raw material contained in the cosmetic composition according to the present invention, is an evergreen crown of lilies and grows at about 90 to 120 cm, and Eurasia is a country of origin. The Greeks have been using Buddha brood rhizomes for the treatment of circulatory diseases since ancient times, and have been used for vascular edema, varicose veins and hemorrhoids. In addition, the microbial broth promotes the digestive function, has a diuretic effect, and makes the blood vessel wall descend and is also used as a herbal tea. In particular, the bruch broth helps smooth circulation and improves the symptoms of chronic venous insufficiency, which is the main cause of swelling and edema of the legs.

The grape seed which is a raw material contained in the cosmetic composition according to the present invention contains about 20 to 30% of fat, about 10 to 15% of protein, about 2 to 3% of the rare (inorganic) content and about 3 to 6% of carbohydrate, 80 to 90%). It is because the tocopherol is 60 to 70 times as many as other extracts, and it has a very strong antioxidant function. It is also known that catechin, which has an antioxidant capacity about 16.5 times higher than tocopherol, contains 8 to 10%, which lowers cholesterol levels and plays a major role in hypertension and atherosclerosis. In addition, grape seeds contain 92 to 95% of proanthocyanidins, which have the strongest antioxidative potential. These substances exist in the form of two, three or four attached together, Also called Oligomeric Proanthocyanidin Complexes (OPCs) or Pycnogenol. Pycnogenol has a powerful antioxidant effect that is 50 times more potent than vitamin C, and has a strong protective function to prevent damage to cell compounds such as lipids, proteins, DNA, and the like.

Arnica, a raw material contained in the cosmetic composition according to the present invention, is a medicinal herb that is effective for perennial herbs, various traumas, swelling and bruises which grow in the central European mountains, which is a genus of Asteraceae distributed in Switzerland and Germany. Arnica gel, a homeopathic theraphy containing arnica extract, has been shown to exert excellent effects in treating pain, bruising, and bruises related to muscles without irritating the skin.

Lemon balm, Melissa officinalis), a raw material contained in the cosmetic composition according to the present invention, is a cold-resistant perennial plant having a height of about 60 to 150 cm, and its stem is square. Leaf length is about 8㎝ wide ovate. It has petiole and hair and strong lemon scent. Flowers are white, yellow and light blue in late summer, and petals are attached to each other. Flowers, leaves and stems can be used. Drinking the leachate after meals helps digestion and can be made into a herbal tea that suppresses sores (gas stomach) and colic. The leachate has a sweating effect and is effective in the treatment of colds and influenza. Leaves and essential oils are used in aromatherapy and are effective for anxiety, depression, insomnia and nervous headaches. Scientific reports that essential oils have anticonvulsant activity are the basis for this use. It also has an antihistamine action and is recommended for allergic patients who develop eczema. It is also a medicinal plant used for promoting menstruation and relieving menstrual pain. Polyphenols and tannins in these plants also have antiviral action. It has also been used as medicine for longevity. If you use the leaves as a bath agent, the lemon incense is similar to the direction, it feels light and comfortable, and the effect of keeping warm and skin is high. You can prevent hair loss by using a myrrh solution as a rinse. Raw leaves reduce pain when applied to insect bites.

The mixture containing the bromelain, budule broth, grape seed, arnica and lemon balm of the present invention may be one prepared by adding bromelain to a mixed extract of a mixture of Bouguer broth extract, grape seed extract, arnica extract and lemon balm , And may be prepared by adding bromelain to a mixed extract obtained by mixing buds of Broome, grape seed, arnica, and lemon balm.

In the present invention, it is preferable to use a mixture prepared by adding bromelain to a mixed extract obtained from a mixture of Bouguer broom extract, grape seed extract, arnica extract and lemon balm extract.

The mixture may have skin convergence, antioxidant and swelling-reducing effects.

The mixed extracts obtained by mixing the above-mentioned microbial extract, grape seed extract, arnica extract and lemon balm extract can be prepared by mixing the extract of Buchbrook, grape seed, arnica and lemon balm in an amount of 1 to 10: 1 to 10: 1 to 10: 1 to 5: 1 to 5: 1 to 5, and most preferably 1: 1: 1: 1.

When the above extracts are mixed in the above-mentioned weight ratio, it may be more advantageous for skin convergence, antioxidation and swelling-reducing effects.

The extraction solvent of the extract of the present invention may be at least one selected from the group consisting of water, anhydrous or hydroalcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol, May be at least one selected from the group consisting of water and anhydrous or hydrated alcohols having 1 to 4 carbon atoms. The anhydrous or hydrated alcohol having 1 to 4 carbon atoms is methanol, ethanol, n-propanol, iso-propanol, n-butanol and tert-butanol. Among the above-mentioned extraction solvents, functional ethanol is most preferably used, and the amount of water contained is preferably 30 to 90% by weight, and most preferably 70% by weight.

The extract of the present invention can be prepared by a known extraction method known to the ordinarily skilled artisan. For example, an extraction solvent having a volume of 1 to 50 times as much as the dry weight of the microbial broth is added and the active ingredient is extracted by immersion at 4 to 30 DEG C for 3 to 20 days, followed by concentrating the extraction solvent with a reduced pressure concentrator . Further, in order to prevent the solvent from evaporating, it is extracted with an extractor equipped with a cooling condenser at 30 to 100 ° C for 1 to 38 hours or at 5 to 30 ° C for 1 to 10 days to extract an active ingredient, Which can be obtained by concentration with a vacuum concentrator. In the above-mentioned heat extraction method, the extraction temperature is preferably 50 to 100 ° C. In addition to the above-described production methods, ultrasonic extraction may be used.

The grape seed extract, arnica extract and lemon balm extract can also be prepared by a known extraction method as described above, and the mixed extract obtained by mixing the broth broth, grape seed, arnica and lemon balm is also the same as the above method , ≪ / RTI >

The mixed extracts obtained by mixing the extract of the present invention with the broth extract of the present invention, the grape seed extract, the arnica extract and the lemon balm extract, or the broth broth, the grape seed, the arnica and the lemon balm are obtained by concentrating the concentrate under reduced pressure, the lyophilized powder or concentrate Spray dried and dried powder.

In the present invention, bromelain is included in an amount of 0.05 to 3% by weight, preferably 0.1 to 0.5% by weight based on the total weight of the mixed extract.

When the bromelain is included in the above range, the effect of skin convergence, antioxidation and swelling can be further improved.

In the cosmetic composition of the present invention, the mixture is contained in an amount of 0.1 to 30% by weight, preferably 0.5 to 20% by weight, in powder or liquid form, based on the total weight of the cosmetic composition. In the above range, skin convergence, antioxidation and swelling reduction effects can be maximized.

The cosmetic composition of the present invention can be used for skin astringency, antioxidation or swelling relief. More preferably, it can be used for swelling relief.

The cosmetic composition of the present invention is not particularly limited in its formulation. For example, it may be formulated such as lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, Lt; / RTI > It may also be a formulation such as a bath cleanser, shampoo, soap, mist or aerosol.

In addition, in the cosmetic composition of each formulation, in addition to the above-mentioned mixture containing the Bromelain extract, Bouguer broom extract, grape seed extract, arnica extract and lemon balm extract, other components may be arbitrarily selected according to the formulation or use purpose of other cosmetics, can do. For example, it may include a coloring agent (coloring matter), a flavoring agent (fragrance), a suspending agent, an emulsifying agent, a solubilizing agent, a stabilizer, an isotonic agent, a pH adjusting agent, a viscosity adjusting agent,

Hereinafter, the present invention will be described in more detail by way of Examples, Experimental Examples and Formulation Examples. However, the following Examples, Experimental Examples and Formulation Examples exemplify the present invention, and the present invention is not limited to the following Examples, Experimental Examples and Formulation Examples.

Manufacturing example  One.

The filtrate was filtered through a filter paper (5C, 185 mm, commercially available from Toyoroshi Kaisha, Ltd., Japan), and the filtrate was concentrated under reduced pressure to remove water. The filtrate was dried at room temperature The broth extract was obtained by aging the broth for 24 hours.

Manufacturing example  2 to 4.

(Preparation Example 2), arnica extract (Preparation Example 3) and lemon balm extract (Preparation Example 2) were carried out in the same manner as in Preparation Example 1, except that grape seed, arnica and lemon balm were used in place of the above- Production Example 4) was obtained.

Example  1. Preparation of the mixture

Each of the extracts prepared in Preparation Examples 1 to 4 was mixed at a weight ratio of 1: 1: 1: 1 to prepare a mixed extract. Then, 0.2% by weight of bromelain was added to the mixed extract to the total weight of the mixed extract to finally prepare a mixture.

Comparative Example  1. Preparation of the mixture

The broth broth extract, grape seed extract, arnica extract and lemon balm extract prepared in Preparation Examples 1 to 4 were mixed at a weight ratio of 1: 1: 1: 1 to prepare a mixture.

Experimental Example  1. Antioxidant effect measurement

DPPH contains a radical in the molecule and is reduced by radicals and aromatic amines. The dark color is discolored, which is measured by the hydrogen donating ability of the antioxidant. The process of decolorization can be explained by the reduction process, and the larger the reducing power, the greater the antioxidant capacity. DPPH used for hydrogen donating ability measurement is a safe free radical, and it shows maximum absorption value at around 517 nm due to non-covalent electrons.

Therefore, the DPPH scavenger activity was measured to measure the antioxidant effect.

The mixtures prepared in Example 1 were prepared at concentrations of 6.25, 12.5, 25, 50 and 100 μL / mL, respectively.

0.2 mL of the above-mentioned concentration was added to 0.1 mL of the DPPH solution at a concentration of 0.2 mM, and the mixture was reacted at room temperature for 20 minutes. Thereafter, the absorbance at 517 nm, that is, the radical scavenging activity, The results are shown in Table 1 below.

[Equation 1]

The concentration (μL / mL) of Example 1 Scavenging Activity (%) 100 95.2 50 78.0 25 63.17 12.5 52.1 6.25 33.02 0 0.0

From the results shown in Table 1, it was confirmed that the mixture of Example 1 containing bromelain, Buchbrook extract, grape seed extract, arnica extract and lemon balm extract had excellent antioxidant ability.

Experimental Example  2. Measurement of skin convergence ability

Skin convergence refers to the phenomenon that skin proteins contract with the polymeric flavonoids to form crosslinks, causing the skin to contract.

In order to measure the astringent effect, the blood protein (Hemoglobin from Bovine, Sigma co., USA) similar to the skin protein was mixed with the mixture prepared in Example 1 at the same weight ratio, and the concentration 100, 500 and 1000 μL / mL, respectively.

Subsequently, the mixture was centrifuged at 1500 rpm for 3 minutes, and the absorbance of the substance was measured at 407 nm. Astringent effect was obtained using the following equation (2) Respectively.

&Quot; (2) "

Mixture (Example 1) Concentration (μL / mL) Astringent Activity (%) 1000 87.3 500 73.1 100 40.3 50 17.2 0 0

From the results shown in the above Table 2, it was confirmed that the mixture containing bromelain, Buchbrook extract, grape seed extract, arnica extract and lemon balm extract had excellent skin convergence ability.

Experimental Example  3. Measurement of swelling relieving ability

The cream of Comparative Formulation Example 1, Comparative Formulation Example 2 and Formulation Example 1 was prepared with the ingredients shown in Table 3 below in order to measure the swelling-reducing ability.

(Unit: wt%) ingredient Comparative Formulation Example 1 Comparative Formulation Example 2 Formulation Example 1 The mixture of Example 1 - - 5.00 The mixture of Comparative Example 1 - 5.00 - Butylene glycol 2.00 2.00 2.00 glycerin 4.00 4.00 4.00 Potassium hydroxide 0.10 0.10 0.10 Tocopheryl acetate 1.0 1.0 1.0 Liquid paraffin 5.0 5.0 5.0 Squalane 5.0 5.0 5.0 Macadimia nut oil 10.0 10.0 10.0 Polysorbate 60 1.0 1.0 1.0 Sorbitan sesquioleate 1.0 1.0 1.0 Carbomer 0.3 0.3 0.3 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water Balance Balance Balance system 100.00 100.00 100.00

To exclude the effect of natural swelling decrease with time, 20 men and 20 women aged 20 to 60 years or older were treated with the cream of Comparative Formulation Example 1, Comparative Formulation Example 2 and Formulation Example 1 on the left side and right side of the eye, The test was applied for 4 weeks at random. The creams of Comparative Formulation Example 1, Comparative Formulation Example 2 and Formulation Example 1 were applied to the eyes and eyes (balls) as the test sites, and the application times were applied twice a day (morning and evening) , dinner).

The subjects were rubbed with a paper towel after cleansing using the same cleanser to remove moisture, and they were stabilized under constant temperature and humidity conditions (20 to 24, 40 to 60% RH) for 30 minutes.

A half of the hypothetical line connecting the lip commissure and the tragus was defined as the measurement site so that measurements could be made at the same site for each evaluation

MoistureMeter D Compact, a moisture analyzer for skin layers, is a device that measures the dielectric constant of a 2.5-mm deep tissue by generating a low-energy electromagnetic field. The measured dielectric constant of the tissue is the moisture The moisture content of the skin before and after 4 weeks of use using a MoistureMeter D Compact instrument is shown in Table 4 below. (%), Which is a change in moisture content, is shown in Table 5 below.

(Unit: tissue water content%) Comparative Formulation Example 1 Comparative Formulation Example 2 Formulation Example 1 After using the eye pod (4 weeks) 54.23 51.78 47.26 After using the ball (4 weeks) 48.63 46.12 44.57

(unit : %) Comparative Formulation Example 1 Comparative Formulation Example 2 Formulation Example 1 After using the eye pod (4 weeks) 0 4.51 12.86 After using the ball (4 weeks) 0 5.16 8.35

In addition, skin volume change (mm ³ ) after 4 weeks of use was measured using Antera 3D ^TM (Miravex, Ireland), which is a device for confirming the change in the topology of the skin. The device is a device that can measure the epidermis and dermis state using a multiwavelength LED light source. It can reconstruct 2-D and 3-D images using built-in programs and can analyze by wavelength.

 The results of the measurement are shown in Table 6, and the skin volume improvement ratio (%), which is the swelling improvement effect, is shown in Table 7 below.

(Unit: mm ³ ) Comparative Formulation Example 1 Comparative Formulation Example 2 Formulation Example 1 After using the eye pod (4 weeks) 166.59 166.11 163.27 After using the ball (4 weeks) 140.39 131.57 124.36

(unit : %) Comparative Formulation Example 1 Comparative Formulation Example 2 Formulation Example 1 After using the eye pod (4 weeks) 0 0.29 2.00 After using the ball (4 weeks) 0 6.29 11.41

From the results of Tables 4 to 7, it was confirmed that the cream of Formulation Example 1 including the mixture of Example 1 was excellent in the swelling improvement effect, and the comparative formulation example containing the mixture of Comparative Example 1 containing no bromelain 2 showed that the swelling improvement effect was not superior to that of the formulation 1.

That is, it was confirmed that the cosmetic composition of the present invention including bromelain exhibited excellent swelling improvement effect.

Therefore, the cosmetic composition of the present invention comprising a mixture containing bromelain, budurobum extract, grape seed extract, arnica extract and lemon balm extract as an active ingredient has excellent effects on antioxidation, skin convergence and swelling relief. , And it can be seen that it exerts excellent effects especially on swelling reduction.

Claims (9)

A cosmetic composition comprising, as an active ingredient, a mixture comprising an extract comprising at least one of Bromelain, Root Extract, Root Extract, Arnica Extract, Lemon Balm Flower, Leaf and Stem. delete [2] The method of claim 1, wherein the mixture comprises an extract comprising at least one of a root extract, a grape seed extract, an arnica extract and a lemon balm flower, leaf and stem of a budgerch's broth at a ratio of 1 to 10: 1 to 10: 1 to 10: To 10% by weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the bromelain is contained in an amount of 0.05 to 3% by weight based on the total weight of the mixture. The cosmetic composition according to claim 1, wherein the mixture is contained in an amount of 0.1 to 30% by weight based on the total weight of the cosmetic composition. [Claim 2] The method according to claim 1, wherein the extraction solvent of the extract is at least one selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol . [Claim 7] The cosmetic composition according to claim 6, wherein the extraction solvent of the extract is at least one selected from the group consisting of water and anhydrous or hydrated alcohol having 1 to 4 carbon atoms. [Claim 2] The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin astringency, antioxidation or swelling relief. The cosmetic composition according to claim 1, wherein the cosmetic composition is a formulation of lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, ultraviolet ray blocking cream, bath detergent, shampoo, soap, mist or aerosol ≪ / RTI >

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