JP2001048768A - Cosmetic, quasi-drug, drug and food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic safely applicable to human body and containing an effective component suitable for cosmetic, quasi-drug, drug or food. SOLUTION: The objective cosmetic, quasi-drug, drug and food formulated with a solvent extract of the root of Rhodiola rosea and/or the root of Paconia anomala exhibit antioxidation action and bleaching action and are effective for preventing the skin roughening, improving the luster and darkness of the skin and imparting the skin with firmness and whiteness.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly safe cosmetic, quasi-drug, medicinal product or food which has a high whitening effect and an active oxygen suppressing effect and is effective for whitening and rough skin.

[0002]

[Prior Art] Iwabenkei is Rhodio
It is called la rosea, and is also known as Iwakirin. North of central Japan, it grows on windy gravel and rocky hills in Takayama, and is also common near Siberia. It is 4 to 35 cm tall and is dioecious, with the female strain often being purplish-brown. It is also used for medicinal purposes. Also, Paeonia anomala
Is used as a sedative in a family of peonies in the genus Button.

[0003]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a cosmetic composition which is safe when applied to the human body and contains effective ingredients required for cosmetics, quasi-drugs, pharmaceuticals and foods. It is in.

[0004]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have screened and examined plants which have been used for food for many years and which have been confirmed to be safe for the human body. , Quasi-drugs, pharmaceuticals, and foods with useful value were examined. As a result, the inventors have found that the solvent extract of the roots of Iwabenkei root and Paeonia anomala is effective as a raw material for cosmetics, or as a quasi-drug, a drug, or a raw material for food, and completed the present invention. It is.

[0005]

The confirmed effects of the solvent extract of Iwabenkei root used as the cosmetic of the present invention are a whitening effect, an active oxygen suppressing effect and an antioxidant effect.

The active oxygen suppressing action will be described in more detail. In general, organisms (except anaerobic ones) cannot exist without oxygen in the air. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. This active oxygen
Oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. In addition, it is said that the generated peroxide and active oxygen damage DNA and promote aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics.

The roots of Iwabenkei and Paeonia anomala are used for extraction with water or a hydrophilic organic solvent such as ethanol, methanol, acetone or the like. However, since it is extraction of raw materials for cosmetics, quasi-drugs, medicines, and foods, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable. In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can be used for the extraction. Furthermore, it is also effective to freeze-dry and use as a powder depending on the method of use.

This substance is used as a raw material for other cosmetics, quasi-drugs, pharmaceuticals, and foods, for example, liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, glycerin, Cosmetics, quasi-drugs, pharmaceuticals, foods such as lotions, creams, emulsions, packs, tablets, drinks, etc. by incorporating humectants such as 3,3-butylene glycol and various drugs, etc. It can be prepared into cosmetics, quasi-drugs, pharmaceuticals, foods and the like in various forms of use depending on the conditions.

[0009]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Iwabenkei no Nemoto and Paeo used in the present invention are described below.
Examples of the production of nia anomala root extract and practical examples of use are described, but the present invention is not limited to these production examples and examples.

Production Example 1 Iwabenkei no Root (Dried product) 10 g
Then, 300 ml of ethanol was added thereto, and the mixture was left for 5 days with occasional stirring. This was filtered, concentrated under reduced pressure, and freeze-dried.

[Production Example 2] Iwabenkei no Root (Dried product) 10 g
Was added to the mixture, and the mixture was left for 5 days with occasional stirring. After filtration and concentration under reduced pressure,
Lyophilized.

[Production Example 3] Iwabenkei no Root (Dried product) 10 g
500 ml of purified water was added thereto and heated with occasional stirring. After cooling, the mixture was filtered and freeze-dried.

[Production Example 4] 300 g of ethanol was added to 10 g of Paeonia anomala root (dry product), and the mixture was left for 5 days with occasional stirring. This was filtered, concentrated under reduced pressure, and freeze-dried.

Production Example 5 300 ml of a 50% aqueous ethanol solution was added to 10 g of Paeonia anomala root (dried product), and the mixture was left for 5 days with occasional stirring. This was filtered, concentrated under reduced pressure, and freeze-dried.

Production Example 6 500 ml of purified water was added to 10 g of Paeonia anomala root (dry product), and the mixture was heated with occasional stirring. After cooling, the mixture was filtered and freeze-dried.

Example 1 (Preparation of lotion) The following components were mixed to prepare a lotion according to a conventional method. Olive oil 0.5 Extract of Preparation Example 1 0.5 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hydrogenated castor oil 2.0 Ethanol 10 1.0 1.0% aqueous sodium hyaluronate solution 5.0 Purified water 80.0

[Example 2 (Preparation of cream)] A and B comprising the following components were each heated to 70 ° C., and then A was gradually added to B while stirring, and then slowly stirred. While cooling to 30 ° C., a cream was prepared. (% By weight) A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production example 2 Extract 1.0 B Purified water 47.9 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0 Glycerin 5.0 1.0 % Aqueous sodium hyaluronate 5.0 Methyl parahydroxybenzoate 0.1

Example 3 (Preparation of lotion) The extract of Production Example 1 in Example 1 was prepared by changing the extract of Production Example 3.

Example 4 (Preparation of Cream) Example 2
Was prepared by changing the extract of Production Example 2 to the extract of Production Example 4.

Example 5 (Preparation of lotion) The extract of Production Example 1 in Example 1 was prepared by changing the extract of Production Example 5.

Example 6 (Preparation of Cream) Example 2
Was prepared by changing the extract of Preparation Example 2 to the extract of Preparation Example 6.

[Example 7 (Preparation of tablet)]
0 g, 30 g of crystalline cellulose, 20 g of lactose, and 1 g of stevia were mixed to form a 2 g tablet.

Example 8 (Preparation of Drink) Example 1
5g, reduced maltose 5g, vitamin C1g, vitamin B
₂ 0.05 g, vitamin B ₆ 0.05 g, 100 with purified water
ml. 1 g of Stevia was made into a 5 g tablet.

Example 9 (Preparation of tablet) The extract of Preparation Example 1 in Example 7 was replaced with the extract of Preparation Example 3 to prepare a tablet.

Example 10 (Preparation of Drink) The extract of Preparation Example 2 in Example 8 was prepared by changing the extract of Preparation Example 4.

Example 11 (Preparation of tablet) The extract of Preparation Example 1 in Example 7 was replaced with the extract of Preparation Example 5 to prepare a tablet.

Example 12 (Preparation of Drink) The extract of Preparation Example 2 in Example 8 was prepared by changing the extract of Preparation Example 6.

Active oxygen suppression effect test pH 8.2 buffer (sodium dihydrogen phosphate 65 mM,
0.2 ml of 35 mM sodium borate, 0.5 mM disodium ethylenediaminetetraacetate), 0.2 ml of 0.5 mM xanthine, 0.1 ml of 1 mM aqueous solution of hydroxylamine hydrochloride and 0.1 ml of water were added to a vial and stirred. , 0.1 ml of the sample is added, and 0.1 ml of water is further added and stirred,
Xanthine oxidase aqueous solution (made of milk, 6unit / m
l) was added and the mixture was allowed to stand at 37 ° C. for 30 minutes.
2.0 ml of the coloring solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes to 18 minutes.
The absorbance at 550 nm was measured during 0 minutes. The results are shown in Table 1 (the water was measured instead of the sample, and the blank was used).

[0029]

Antioxidant test 0.5 ml of β-carotene solution (β-carotene 100 mg / 100 ml chloroform), linoleic acid solution (linoleic acid 10 g / 10
0 ml chloroform) 0.2 ml, Tween (40) solution (T
Wean (40) 20 g / 100 ml chloroform) 1.0 ml 20
After taking chloroform into a 0 ml Erlenmeyer flask and completely removing chloroform with nitrogen gas, 100 ml of purified water is added and dissolved to prepare a linoleic acid-β-carotene solution. 2. To 45 ml of the linoleic acid-β-carotene solution was added 4 ml of a 0.2 M phosphate buffer (pH 6.8), and the mixture was gently stirred.
Was dispensed into a test tube, 0.1 ml of a 50% aqueous solution of a 0.05% specimen in ethanol was added thereto, and the mixture was quickly transferred to a reaction vessel at 50 ° C., and after 10 minutes and 40 minutes, the O.D. D. Measure 470 nm. The formula for calculating the antioxidant power is shown below. Antioxidant power = {(AB) − (CD)} / (AB) ×
100 where A = absorbance 10 minutes after control B = absorbance 40 minutes after control C = absorbance 10 minutes after sample D = absorbance 40 minutes after sample

The results are shown in Table 2.

Tyrosinase activity inhibition test (tyrosine substrate) (Test method) Phosphate buffer (pH 6.8, 30 mM)
0.9 ml, 1.66 mM tyrosine (1.0 ml) solution, 0.1 ml of the water or dimethyl sulfoxide solution of Example, and 0.9 ml of purified water were placed in a screw vial, and heated in a 37 ° C. constant temperature water bath for 5 minutes or more. Tyrosinase solution (Sigma,
0.1 ml of mushroom-derived, 914 units / ml)
The solution was kept in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes. As a control, pure water or dimethyl sulfoxide was added instead of the above sample solution, and the measurement was performed in the same manner. (Calculation formula) Tyrosinase activity inhibition rate (tyrosine substrate) = {B- (AP)} / B x 100 where A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3-fold dilution) Result Are shown in Table 3.

[0033]

Tyrosinase activity inhibition test (DOPA substrate) (Test method) Phosphate buffer (pH 6.8, 30 mM)
1.8 ml, 0.05% L-β- (3,4-dihydroxyphenyl) alanine (L-β- (3,4-Dihydroxy-phenyl) alan)
ine) 1.0 ml of the solution, 0.1 ml of the water or dimethylsulfoxide solution of the example was placed in a screw vial, and placed at 25 ° C.
The temperature was kept at least 10 minutes in a water bath. 0.1 ml of a tyrosinase solution (manufactured by Sigma, from mushrooms) was added, stirred and after 30 seconds the absorbance at 475 nm was measured 11 times every 15 seconds. (While maintaining the absorbance measurement cell at 25 ° C.) As a control, pure water or dimethyl sulfoxide was added instead of the above sample solution, and the measurement was performed in the same manner. Table 4 shows the results.

[0035]

Animal whitening test Hair from the back of a colored guinea pig (female, 6 animals) was shaved and shaved with an electric clipper and an electric shaver from the back of the guinea pig (6 females). :ethanol:
A solution of propylene glycol (4: 4: 2) was treated with 0.05
Each ml was applied twice a day. As a control, a solution of purified water: ethanol: propylene glycol = 4: 4: 2 was similarly performed. UV light (UV-B) from the second day 3 per week
Irradiation was performed at 750 mJ for 2 weeks. From the second day, L * (brightness) and MI (melanin index) were measured by a color difference meter at intervals of two weeks. Table 5 shows the results.

[0037]

As a result, in both L * and MI, blackening was significantly suppressed as compared with the control.

(Usage Test 1) The face of each of seven women was divided into left and right sides, and the lotion and cream of the example were set on one side, and the lotion and cream of the comparative example were set on the other side, once a day. Three months after the above use, the skin was evaluated for whitening, prevention of rough skin, improvement of skin gloss, blemishes, and skin abrasion. In Comparative Examples, various extracts of Production Examples were replaced with water from Examples (Comparative Examples 1 and 2).
2). In addition, 21 persons were divided into three groups and tested using the samples shown in Table 6 below.

The evaluation was made according to the following evaluation criteria, and the results are summarized in Table 7 below. (Evaluation criteria) Example is very good 3 Example is considerably better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better − 2 Comparative example is much better -3

[0041]

(Use Test 2) Five women were asked to eat two tablets of the example and 100 ml of a drink once a day every day for three months. In addition, 15 persons were divided into three groups and tested using the samples shown in Table 8 below.

The evaluation was performed according to the following evaluation criteria, and the results are summarized in Table 9 below. (Evaluation criteria) After the test is very good 3 After the test is considerably better 2 After the test is slightly better 1 There is no difference 0 Before the test is slightly better -1 Before the test is much better − 2 Very good before test -3

[0044]

[Effect] Cosmetics, quasi-drugs, pharmaceuticals and foods containing solvent extract of Iwabenkei root and / or Paeonia anomala root exhibit antioxidant and whitening effects, prevent rough skin, It is effective in improving gloss, blemishes, skin radiance and whitening.

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[Procedure amendment]

[Submission date] August 10, 1999 (1999.8.1
0)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0003

[Correction method] Change

[Correction contents]

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic or quasi-drug containing an effective ingredient required for cosmetics, quasi-drugs, pharmaceuticals and foods, while being safe to apply to the human body. Product, medicine and food.

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0022

[Correction method] Change

[Correction contents]

[Example 7 (Preparation of tablet)]
0 g, 30 g of crystalline cellulose, 20 g of lactose, and 1 g of stevia were mixed to form a 2 g tablet.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0023

[Correction method] Change

[Correction contents]

Example 8 (Preparation of Drink) Production Example 1
5g, reduced maltose 5g, vitamin C1g, vitamin B
₂ 0.05 g, vitamin B ₆ 0.05 g, 100 with purified water
ml.

Continued on the front page F term (reference) 4B018 MD23 MD25 MD32 MD42 MD61 ME06 ME14 MF01 MF02 4C083 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC422 AC432 AC442 AC482 AD212 AD262 AD332 AD392 AD632 AD642 CC01 CC05 DD15 DD23 DD31 EE12 AB03 AC11 MA07 MA63 NA14 ZA89

Claims (1)

[Claims] 1. The root of Iwabenkei and / or Paeonia anom
Cosmetics, quasi-drugs, blended with ala root solvent extract,
Pharmaceuticals, food