JPH07118135A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic containing solvent extracts of Adhatoda vasica used for food over long years and being safe to human body as active ingredients, having large whitening action and inhibiting the activity of hyaluronidase. CONSTITUTION:This cosmetic contains extracts obtained by extracting Adhatoda vasica which is an evergreen short tree being a plant of Acanthaceae and cultured in the tropics with water or ethanol or mixed solvent thereof as active ingredients. The cosmetic has high whitening action and inhibits hyaluronidase activity and it is effective in chapped skin and high in safety. This cosmetic can be prepared in various types of cosmetics, e.g. lotion, cream, milky lotion and pack by blending this cosmetic with other cosmetic raw material, e.g. squalane, jojoba oil, beeswax, cetyl alcohol, various kinds of activating agents, humectant such as glycerin and various kinds of medicines, etc. This cosmetic suppresses tyrosinase activity, hyaluronidase activity and active oxygen and it is effective in chapped skin or tension of skin.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition which has a high whitening effect, inhibits the activity of hyaluronidase and is effective against rough skin.

[0002]

[Prior Art] Adhatoda vasica
Is a plant belonging to the genus Adatoda of the family Lepidoptera, distributed in Punjab, Assam, India, Sri Lanka, and Singapore, and is an evergreen shrub cultivated in various tropical regions. This adatda bashika is an important medicinal plant that has been used for over 2000 years as a therapeutic drug for asthma, fever, jaundice, blood purification, etc. in India.

On the other hand, various substances are known as substances having a whitening effect which can be used as a raw material for cosmetics, but synthetic products have no guarantee of safety when applied to human skin for a long period of time. , Its use is being limited. On the other hand, many natural products have a weak whitening effect. However, from the viewpoint of safety for human skin, it is a natural product, and people eat it for many years,
Guaranteed in terms of safety and strong whitening effect,
Furthermore, a substance that also has other effects on the skin has been desired.

[0004]

The object of the present invention is to provide a cosmetic composition which is safe when applied to the skin, has a large whitening effect, inhibits the activity of hyaluronidase, and further contains an ingredient effective for rough skin and the like. To provide.

[0005]

[Means for Solving the Problems] In order to solve the above-mentioned problems, the present inventors have screened and investigated plants that have been used for food for many years and have been confirmed to be safe for the human body. I examined what is useful as. As a result, they have found that Adatoda bashika is effective as a raw material for cosmetics or as a quasi drug, and completed the present invention. That is, the present invention is a cosmetic containing a solvent extract of Adatda bashika.

[0006]

The action of the solvent extract of Adatda vashika used as the cosmetic of the present invention is as follows: first, the skin whitening action, second, the hyaluronidase activity suppressing action, and the third, active oxygen suppressing action, Fourth is the antioxidant effect. Second above
The activity of hyaluronidase for suppressing the activity will be described in more detail. Hyaluronidase is widely distributed in the living body,
It is an enzyme that is also present in the skin, and as its name implies, it decomposes hyaluronic acid. Hyaluronic acid is a linear polymer polysaccharide in which β-D-N-acetylglucosamine and β-D-glucuronic acid are alternately bound, and is a glycosaminoglucan that widely exists in connective tissues of mammals along with chondroitin sulfate and the like. Is a kind of. The action of hyaluronic acid in connective tissue is to retain water in the intercellular spaces, form a jelly-like matrix in the tissue to retain cells,
It is thought to maintain the lubricity and flexibility of the skin and prevent external forces (mechanical damage) and bacterial infections. In addition, it is said that hyaluronic acid in the skin decreases with age, resulting in aging such as wrinkles and bulkiness. Therefore, suppressing the activity of hyaluronidase that decomposes this hyaluronic acid, the stability of hyaluronic acid used in the formulation, the hyaluronic acid of the formulation after application to the skin and the hyaluronic acid present in the skin It is thought to contribute to stability.

The third active oxygen suppressing action will be described in more detail. Generally, no organisms (except anaerobic ones) can exist without oxygen in the air. But,
Oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes.
This active oxygen oxidizes a fatty acid and produces a peroxide. It also oxidizes and damages the phospholipids of biological membranes in the body. Furthermore, it is said that the generated peroxide and active oxygen damage DNA and accelerate aging. This active oxygen also influences the mechanism of making melanin from tyrosine and is also involved in the blackening of the skin. Suppressing this active oxygen is an important factor for the skin, in other words, an important factor required for cosmetics.

As a method for utilizing Adatda bashika, extraction is carried out with water or a hydrophilic organic solvent such as ethanol, methanol or acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable because it is extraction of cosmetic raw materials. In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol or a mixed liquid of polyhydric alcohol and water can be used for extraction. Furthermore, freeze-drying and using it as a powder is also effective depending on the method of use.

This substance is used as a raw material for other cosmetics, for example, liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3-butylene glycol and various Cosmetics of various dosage forms such as lotions, creams, etc.
It can be prepared into a milky lotion, a pack or the like, and can be prepared into cosmetics of various usage forms according to the purpose.

[0010]

[Examples] The following is a description of production examples of the extract of Adatda vashika used in the present invention and examples of actual utilization methods, but the present invention is in no way limited by these production examples and examples. Not a thing.

[Production Example 1] To 10 g of the Adatda vashika wood (dry product) was added 300 ml of ethanol, and the mixture was left for 5 days with occasional stirring. This was filtered and freeze-dried.

[Production Example 2] To 10 g of Adatda vashika wood (dry product) was added 300 ml of 50% ethanol aqueous solution, and the mixture was left for 5 days with occasional stirring. This was filtered and freeze-dried.

[Production Example 3] To 10 g of Adatda vashika wood (dry product), 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

[Example 1 (Preparation of lotion)] The following components were mixed to prepare a lotion by a conventional method. (% By weight) Olive oil 0.5 Ethanol extract of Adatda vashika wood of Production Example 0.5 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) Hardened Castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

[Example 2 (Preparation of cream)] A and B each consisting of the following components were heated to 70 ° C., and then A was slowly added to B with stirring and then slowly stirred. While cooling to 30 ° C, a cream was prepared. (Wt%) A squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production example 2 50% ethanol extract of Adatoda bashika wood 1.0 B Purified water 47.9 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1 0.0 Glycerin 5.0 1.0% sodium hyaluronate aqueous solution 5.0 Methyl paraoxybenzoate 0.1

[Example 3 (Preparation of cream)] Example 2
Was prepared by replacing the extract of Production Example 2 of Example 2 with the extract of Production Example 3.

[Inhibition of tyrosinase activity] (Test method) McClevaln buffer solution 0.9 m
l, 1.66 mM Tyrosine solution 1.0 ml, 0.1 wt / v% aqueous solution of the above production example (freeze-dried product) (if it is difficult to dissolve, add ethanol and dissolve, then add purified water and evaporate , After removing ethanol, 0.1w
1.0 ml (prepared to be t / v%) was placed in a screw vial and heated in a 37 ° C. constant temperature water bath for 5 minutes or more. Tyrosinase solution (Sigma, mushroom-derived, 914
0.1 unit (ml / ml) was added, and the temperature was kept in a 37 ° C. constant temperature water bath, and after 10 minutes, the absorbance was measured at 475 nm. As a control, pure water was added instead of the sample solution and the same measurement was performed.
In this test, the final concentration of the sample is 0.033%. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} /
B × 100 However, A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3-fold dilution)

[0018]

[Table 1]

[Hyaluronidase activity inhibition test] (Test method) 0.4% sodium hyaluronate 0.1M
(PH 6.0) 6 g of phosphate buffer solution was weighed and allowed to stand in a 37 ° C. constant temperature water bath for 5 minutes, and then 0.1 wt / v% aqueous solution of the above production example (lyophilized product) In addition, after dissolving, purified water was added to the solution to evaporate it, and ethanol was removed. Then, the solution was adjusted to 0.1 wt / v%. 1.0 ml was added and stirred, and 0.01% hyaluronidase (manufactured by Sigma) Made of testicles, type I-S)
1 ml of 0.1 M (pH 6.0) phosphate buffer was added and immediately stirred, and 6 ml was placed in an Ostwald viscometer placed in a 37 ° C. constant temperature water bath. The viscosity was measured after 1 minute, 5 minutes, 10 minutes, 20 minutes, and 40 minutes. As a control, pure water was added instead of the above sample solution, and the same measurement was performed. In this test, the final concentration of each sample is 0.0125% of the concentration of the analyte. The results are shown in Table 2 below as an index, with the viscosity after 1 minute being 100.

[0020]

[Table 2]

[Active Oxygen Suppression Test] There are various methods for measuring the effect of suppressing active oxygen, but the following method was used this time. pH 7.8 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml Catalase above potassium phosphate buffer 5 ml 2 mM Nitroblue tetrazolium above potassium phosphate buffer 5 ml 1.8 mM Xanthine above phosphorus Potassium acid buffer 17 ml 160 ml

2.4 ml of the mixture of the above reagents and 0.3 ml of the sample
In addition, xanthine oxidase (using water as the sample beforehand,
When conducting an experiment, add 0.3 ml of the above solution so that the absorbance will increase to around 0.02 per minute (adjusted with the above potassium phosphate buffer solution) and immediately measure the absorbance (560 nm) , Check the linearity). (Calculation formula) Inhibition rate = {(A−B) / A} × 100 where A: change in absorbance per minute when the sample is water B: change in absorbance per minute of sample Several steps were carried out to find the 50% active oxygen production inhibitory concentration. The preparation method of the sample is to prepare an aqueous solution of the above preparation example (freeze-dried product) at an appropriate concentration (if it is difficult to dissolve, add ethanol and dissolve it, then add purified water and evaporate to remove ethanol. It was prepared so that the concentration became%). The results for Production Examples 1 and 2 are shown in Table 3 below.

[0023]

[Table 3]

[Antioxidant test] With the following screw cap 50
Made into ml test tubes. Specimen 5 ml 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml Purified water 5 ml This 50 ml test tube with a screw cap is left in a thermostat bath at 50 ° C in the dark. Before putting this in a constant temperature bath, 3 days later, 6
The following measurements were made after 8 days. Test solution 0.125m
1, 12.25 ml of 75% ethanol and 0.125 ml of 30% ammonium thiocyanate were added and stirred and left for 3 minutes, and then 0.125 ml of 0.02N ferrous chloride 3.5% HCL aqueous solution was added and stirred for 3 minutes. Wavelength 50 after leaving
Absorbance was measured at 0 nm. The cell length was 10 mm, and the control cell had the test solution replaced with water. The results are shown in Table 4 below.
5 shows.

[0025]

[Table 4]

[0026]

[Table 5]

(Usage Test) Faces of 6 women were divided into left and right sides, and the lotion and cream of the example were set on one side, and the lotion and cream of the comparative example were set on the other side and used once or more daily. After three months,
The whitening, the prevention of rough skin, the gloss of the skin and the suppleness of the skin were evaluated. In addition, the comparative example is the one in which the various extracts of Adatoba bashika in the production examples are replaced with water in the production examples (Comparative Examples 1 and 2). In addition, 12 people were divided into 2 groups and tested using the samples shown in Table 6 below.

[0028]

[Table 6]

The evaluation is made according to the following evaluation criteria, and the values obtained by summing the evaluation scores are summarized in Table 7 below. (Evaluation Criteria) The Example is Better 3 The Example is Better 2 The Example is Better 1 No Difference 0 The Comparative Example Better -1 The Comparative Example Better- 2 The comparative example is much better -3

[0030]

[Table 7]

Results of tyrosinase activity inhibition test (Table 1), hyaluronidase activity inhibition test result (Table 2), active oxygen inhibition test result (Table 3), antioxidant test (Tables 4 and 5), use test (Table) As is clear from 7), the cosmetic containing the solvent extract of Adatda vashika of the present invention inhibits the activity of tyrosinase, the activity of hyaluronidase and active oxygen, and is found to be effective for rough skin, skin blisters and the like. It was

[0032]

EFFECTS OF THE INVENTION According to the present invention, there is provided a highly safe cosmetic composition which has a high whitening effect, suppresses the activity of hyaluronidase, and is effective against rough skin.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masao Hattori 2556-4-22-203 Gofuku-Suehiro-cho, Toyama City, Toyama Prefecture (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture Nakamura Masami 6-32 Ikegami Town, Toba City, Mie Prefecture

Claims (1)

[Claims] 1. Adhatoda vasica
Cosmetics containing the solvent extract of.