JP3792745B2 - Tyrosinase inhibitor - Google Patents

Description

【００１８】
【表１】

【００１９】
使用テスト
女性６名づつの顔面を左右に分け、一方を実施例、もう一方を比較例として毎日、１回以上使用してもらって、３月後、アンケートした。なお、比較例は実施例５、６よりそれぞれ実施例１、２による溶媒抽出物を水にかえたものである。（比較例１，２）
なお、１２名を２班にわけ、下記の試料を使って実験した。

【００２０】
判定基準は以下のようでアンケートの結果をまとめたのが以下の表である。
（なお、数値は判定結果の合計点で示した）
実施例の方が非常によい ３
実施例の方がかなりよい ２
実施例の方がややよい １
差がない ０
比較例の方がややよい −１
比較例の方がかなりよい −２
比較例の方が非常によい −３
【００２１】

【００２２】
【効果】
本発明の栗の葉、栗のいがの溶媒抽出物、或いはそれを配合したクリーム、ローション等のチロシナーゼ活性阻害剤は優れたチロシナーゼ活性阻害を示し、美白効果を有する。また、これらは長年にわたって利用されてきており、その安全性については保証されている。[0001]
[Industrial application fields]
The present invention relates to a tyrosinase inhibitor that is effective for whitening the skin by suppressing the activity of tyrosinase using a plant extract that has been used for many years as a medicinal product for other purposes and whose safety is guaranteed.
[0002]
[Prior art]
Chestnut is a deciduous tree that is distributed from southwestern Hokkaido to Kyushu and the Korean peninsula. Is done.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide a raw material that is safe to apply to the skin and has a strong tyrosinase inhibitory action.
Tyrosinase is an enzyme present in the skin, and its function is to produce melanin from tyrosine and blacken the color of the skin. The older white color is said to conceal the seven difficulties and was the desire of many women.
[0004]
[Means for solving the problems]
In order to solve the above problems, the present inventors screened and examined plants that have been used for food for many years and have been confirmed to be safe for the human body, and examined those that are useful as tyrosinase inhibitors. .
As a result, it was found that chestnut leaves and chestnut potatoes are raw materials with a very strong tyrosinase inhibitory action.
[0005]
That is, the present invention is a tyrosinase inhibitor containing a solvent extract of chestnut leaves and chestnut ginger.
[0006]
As a method of using chestnut leaves and chestnut ginger, extraction is performed with water or a hydrophilic organic solvent such as ethanol, methanol, acetone or the like. However, since it is extraction of cosmetic raw materials, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable. In some cases, polyhydric alcohols such as glycerin, 1,3 butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction. Furthermore, it is also effective to freeze-dry and use as powder depending on the method of use.
[0007]
This substance is added to other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various active agents, humectants such as glycerin and 1,3 butylene glycol, and various drugs. Various dosage forms of cosmetics can be adjusted. For example, the use form may be considered according to the purpose, such as lotion, cream, milky lotion, pack and the like.
[0008]
【Example】
Examples which are actual utilization methods will be described below, but the present invention is not limited to these examples.
Examples of chestnut leaves and chestnut garlic extracts used in the present invention are shown below.
[0009]
Example 1
Add 300 ml of purified water to 10 g of chestnut leaves and heat for 3 hours. This was filtered and freeze-dried.
[0010]
Example 2
Add 300 ml of purified water to 10 g of chestnuts and heat for 3 hours. This was filtered and freeze-dried.
[0011]
Example 3
The chestnut was added to 30 g of ethanol and 300 ml of ethanol, and allowed to stand for 5 days with occasional stirring. This was evaporated and then lyophilized.
[0012]
Example 4
Add 10 ml of chestnut ginger (dried product) to 300 g of purified water and heat for 3 hours. This was filtered and freeze-dried.
[0013]
Example 5 Lotion Olive Oil 0.5
Solvent extract according to Example 1 0.5
Polyoxyethylene (20E.0) sorbitan monostearate 2.0
Polyoxyethylene (60E.0) hydrogenated castor oil 2.0
Ethanol 10.0
1.0% sodium hyaluronate aqueous solution 5.0
Purified water 80.0
[0014]
Example 6 Cream A Squalane 20.0
Olive oil 2.0
Mink oil 1.0
Jojoba oil 5.0
Beeswax 5.0
Cetostearyl alcohol 2.0
Glycerol monostearate 1.0
Sorbitan monostearate 2.0
Solvent extract according to Example 2 1.0
B Purified water 47.9
Polyoxyethylene (20E.0) sorbitan monostearate 2.0
Polyoxyethylene (60E.0) hydrogenated castor oil 1.0
Glycerin 5.0
1.0% sodium hyaluronate aqueous solution 5.0
Methyl paraoxybenzoate 0.1
A and B were weighed and heated to 70 ° C., A was gradually added to B while stirring, and then cooled to 30 ° C. with slow stirring.
[0015]
Example-7 was prepared by replacing the extract of Example 1 of Example-5 with the extract of Example 3.
Example-8 was prepared by replacing the extract of Example 2 of Example-6 with the extract of Example 4.
Tyrosinase activity inhibition test (test method)
0.9 ml of 30 mM phosphate buffer (pH 6.8), 1.0 ml of 1.66 mM tyrosine solution, and 0.1 wt / v% aqueous solution of the above-mentioned example (lyophilized product) (if it is difficult to dissolve, ethanol is used. After adding and dissolving, purified water was added, evaporated, ethanol was removed, and adjusted to 0.1 wt / v%) 1.0 ml was taken in a screw vial and kept in a 37 ° C constant temperature bath for 5 minutes or longer Warmed up.
0.1 ml of tyrosinase solution (manufactured by Sigma, derived from Massy Room, 914 units / ml) was added, and the mixture was kept warm in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes.
As a control, pure water was added instead of the sample solution, and the same measurement was performed.
In this test, the final concentration of the sample is 0.033%.
(a formula)

[0018]
[Table 1]

[0019]
Use test 6 female faces were divided into left and right sides, and one was used as an example and the other was used as a comparative example. In the comparative example, the solvent extracts according to Examples 1 and 2 were replaced with water from Examples 5 and 6, respectively. (Comparative Examples 1 and 2)
In addition, 12 people were divided into 2 groups, and the following samples were used for experiments.

[0020]
The criteria are as follows, and the results of the questionnaire are summarized in the following table.
(The numerical value is indicated by the total score of the judgment results)
The example is much better 3
The example is much better 2
Example is slightly better 1
No difference 0
Comparative example is slightly better -1
The comparative example is much better -2
The comparative example is much better -3
[0021]

[0022]
【effect】
The tyrosinase activity inhibitors such as chestnut leaf, chestnut garlic solvent extract of the present invention, or creams and lotions containing the same exhibit excellent tyrosinase activity inhibition and have a whitening effect. In addition, they have been used for many years and their safety is guaranteed.

Claims (1)

  Tyrosinase inhibitor comprising a chestnut leaf and / or chestnut ginger solvent extract as an active ingredient