JPH08217687A - Tyrosinase inhibitor - Google Patents

Abstract

PURPOSE: To obtain the subject imhibitor containing an extract of leaf of Japanese chestnut or bur of Japanese chestnut capable of suppressing activity of tyrosinase inhibitor, having high safety and effective in beautifying skin. CONSTITUTION: This inhibitor contains an extract of leaf of Japanese chestnut or bur of Japanese chestnut with a solvent. Furthermore, e.g. leaf of Japanese chestnut or bur of Japanese chestnut is preferably extracted using water or a hydrophilic organic solvent such as ethanol. Specifically, 300ml purified water is added to 10g leaf of Japanese chestnut and the mixture is heated for 3hr and filtered and then subjected to lyophilization, etc. For example, the inhibitor is added to a cosmetic raw material such as squalane and preferably used as a lotion, etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention has been used for many years as a drug for other purposes, etc., and uses a plant extract of which the safety is assured to suppress the activity of tyrosinase and to effectively whiten the skin. It relates to tyrosinase inhibitors.

[0002]

2. Description of the Related Art Chestnuts are deciduous trees that are distributed from southwestern Hokkaido to Kyushu and the Korean Peninsula and are widely used for food. Their leaves are called chestnut leaves, and their leaves are known as chestnut palms. Used externally for burns, etc.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a raw material which is safe when applied to the skin and has a strong tyrosinase inhibitory action. Tyrosinase is an enzyme that is present in the skin, and its function produces melanin from tyrosine, which darkens the skin color. It is said that the white one, which has been colored since ancient times, is said to hide the seven difficulties, and was a desire of many women.

[0004]

[Means for Solving the Problems] In order to solve the above-mentioned problems, the present inventors screened and examined plants that had been used for food for many years and confirmed to be safe for humans, and used as tyrosinase inhibitors. Considered something of value. As a result, they have found that chestnut leaves and chestnut gall are raw materials having a very strong tyrosinase inhibitory action.

That is, the present invention is a tyrosinase inhibitor containing a solvent extract of chestnut leaves and chestnut potato.

The chestnut leaves and chestnut mushrooms can be used by extraction with water or a hydrophilic organic solvent such as ethanol, methanol or acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable since it is extraction of cosmetic raw materials. In some cases, glycerin, 1,3 butylene glycol,
A polyhydric alcohol such as propylene glycol or a mixed liquid of polyhydric alcohol and water can also be used for extraction. Further, freeze-drying and using it as powder is also effective depending on the method of use.

[0007] This substance is used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various drugs. It can be added to adjust various dosage forms of cosmetics. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

[0008]

EXAMPLE An example of an actual usage will be described below, but the present invention is not limited to this example. Examples of chestnut leaves and chestnut mushroom extracts used in the present invention are shown below.

Example 1 To 10 g of chestnut leaves, 300 ml of purified water was added and heated for 3 hours. This was filtered and freeze-dried.

Example 2 To 10 g of chestnut, 300 ml of purified water was added and heated for 3 hours. This was filtered and freeze-dried.

Example 3 300 g of ethanol was added to 30 g of chestnut and left for 5 days with occasional stirring. This was evaporated and lyophilized.

Example 4 To 10 g of chestnut (dry product), 300 ml of purified water was added and heated for 3 hours. This was filtered and freeze-dried.

Example 5 Lotion Olive Oil 0.5 Example 1 0.5 Folioxyethylene (20E.0) Solhitane Monostearate 2.0 Folioxyethylene (60E.0) Hydrogenated Castor Oil 2.0 Ethanol 10. 0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

Example 6 Cream A Squalane 20.0 Olive Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerin Monostearate 1.0 Sorbitan Monostearate 2.0 Example 2 1.0 B Purified water 47.9 Folioxyethylene (20E.0) solhitanium monostearate 2.0 Folioxyethylene (60E.0) hydrogenated castor oil 1.0 Glycerin 5.0 1.0% Aqueous sodium hyaluronate solution 5.0 Methyl paraoxybenzoate 0.1 Weigh A and B respectively, warm to 70 ° C., slowly add A to B with stirring, then slowly agitate with 30
Cooled to ° C.

Example-7 was prepared by replacing the extract of Example-1 of Example-5 with the extract of Example-3.

Example-8 was prepared by replacing the extract of Example-2 of Example-6 with the extract of Example-4.

Tyrosinase activity inhibition test (test method) 30 mM phosphate buffer (pH 6.8)
9 ml, 1.66 mM Tyrosine solution 1.0 ml, 0.1 wt of the above-mentioned example (lyophilized product)
/ V% aqueous solution (if it is difficult to dissolve, add ethanol to dissolve it, and then add purified water to evaporate and remove ethanol to prepare 0.1 wt / v%) 1.0 ml screw 37 for vial
It was heated in a constant temperature water bath at ℃ for 5 minutes or more. 0.1 ml of a tyrosinase solution (manufactured by Sigma, derived from Massroom, 914 units / ml) was added, and the mixture was kept warm in a 37 ° C. constant temperature water bath, and after 10 minutes, the absorbance was measured at 475 nm. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner. In this test, the final concentration of the sample is 0.033%. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} /
B × 100 However, A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3-fold dilution)

[0018]

[Table 1]

Usage test Each of the six female faces was divided into left and right sides, one of which was used as an example and the other of which was used as a comparative example once or more daily.
After three months, I did a questionnaire. The comparative examples are Examples 5 and 6.
More specifically, Examples 1 and 2 are replaced with water.
(Comparative Examples 1 and 2) In addition, 12 people were divided into 2 groups, and experiments were conducted using the following samples.

The criteria are as follows, and the results of the questionnaire are summarized in the table below. Example is much better 3 Example is much better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

[0021]

[0022]

[Effect] The tyrosinase activity inhibitor of the present invention such as chestnut leaves, chestnut mushroom solvent extract, or creams and lotions containing the same extract shows excellent tyrosinase activity inhibition and has a whitening effect. Moreover, these have been used for many years, and their safety is guaranteed.

Claims (1)

[Claims] 1. A tyrosinase inhibitor containing a solvent extract of chestnut leaves and chestnut mushrooms.