JP3235919B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly safe cosmetic which has a high whitening effect, inhibits the activity of hyaluronidase, and is effective for rough skin.

[0002]

BACKGROUND ART Adhatoda vasica
Is an evergreen shrub that is a plant of the genus Adatoda in the family Papilionaceae, distributed from the Punjab and Assam regions of India to Sri Lanka and Singapore. Adatoda basica is an important medicinal plant that has been used in India and other countries for over 2,000 years as a remedy for asthma, fever, jaundice, blood purification and the like.

[0003] On the other hand, various substances having a whitening effect that can be used as a raw material of cosmetics are known, but synthetic products have no guarantee of safety when applied to human skin for a long time. , Use is being restricted. On the other hand, many natural products have a weak whitening effect. However, it is a natural product from the aspect of safety for human skin, and humans eat it for many years,
It is guaranteed in terms of safety, and has a strong whitening effect,
There is a need for a substance that also has other effects on the skin.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic composition which is safe when applied to the skin, has a large whitening effect, inhibits the activity of hyaluronidase, and further contains an effective ingredient for rough skin. To provide.

[0005]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have screened and examined plants which have been used for food for many years and which have been confirmed to be safe for the human body. We considered something worthwhile. As a result, they have found that Adatoda basica is effective as a raw material for cosmetics or as a quasi-drug, and have completed the present invention. That is, the present invention is a cosmetic containing a solvent extract of Adatoda basilica.

[0006]

The confirmed effects of the solvent extract of Adatoda basilica used as the cosmetic of the present invention are as follows: first, skin whitening effect; second, hyaluronidase activity inhibitory effect; third, active oxygen inhibitory effect. Fourth, there is an antioxidant effect. The second
The activity-suppressing action of hyaluronidase will be described in more detail. Hyaluronidase is widely distributed in living organisms,
It is an enzyme that is also present in the skin and degrades hyaluronic acid as its name suggests. Hyaluronic acid is a linear high molecular polysaccharide in which β-DN-acetylglucosamine and β-D-glucuronic acid are alternately bonded, and is a glycosaminoglucan widely present in connective tissues of mammals together with chondroitin sulfate and the like. Is a kind of The effect of hyaluronic acid in connective tissue is to keep water in the intercellular space, and to form a jelly-like matrix in the tissue to keep cells,
It is believed to maintain the lubricity and flexibility of the skin and prevent external forces (mechanical damage) and bacterial infection. In addition, it is said that hyaluronic acid in the skin decreases with age, which results in aging such as fine wrinkles and dryness. Therefore, suppressing the activity of the hyaluronidase that degrades this hyaluronic acid, the stability of the hyaluronic acid used in the formulation, the hyaluronic acid of the formulation after application to the skin and the hyaluronic acid present in the skin It is thought to contribute to stability.

[0007] The third active oxygen suppressing action will be described in more detail. In general, organisms (except anaerobic ones) cannot exist without oxygen in the air. But,
Oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes.
This active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. In addition, it is said that the generated peroxide and active oxygen damage DNA and promote aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics.

[0008] Adatoda basica is used for extraction with water or a hydrophilic organic solvent such as ethanol, methanol, acetone or the like. However, since it is an extraction of cosmetic raw materials, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable. In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can be used for the extraction. Furthermore, it is also effective to freeze-dry and use as a powder depending on the method of use.

This substance is used as a raw material for other cosmetics, for example, liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin, 1,3-butylene glycol and various other oils. Various formulations of cosmetics, such as lotions, creams,
It can be prepared into emulsions, packs and the like, and can be prepared into cosmetics and the like in various usage forms according to the purpose.

[0010]

EXAMPLES Examples of the production of the extract of Adatoda basilica used in the present invention and examples of actual use are described below, but the present invention is not limited by these production examples and examples. Not something.

Production Example 1 300 g of ethanol (300 g) was added to 10 g of a material of Adatoda basilica (dry product), and the mixture was left for 5 days with occasional stirring. This was lyophilized after filtration.

Production Example 2 300 g of a 50% aqueous ethanol solution was added to 10 g of Adatoda basil material (dry product), and the mixture was allowed to stand for 5 days with occasional stirring. This was lyophilized after filtration.

[Production Example 3] 300 g of purified water was added to 10 g of Adatoda Basica wood (dry product), and the mixture was heated for 3 hours. This was allowed to cool, filtered, and lyophilized.

Example 1 (Preparation of lotion) The following components were mixed to prepare a lotion according to a conventional method. (Wt%) Olive oil 0.5 Ethanol extract of Adatoda basil wood of Preparation Example 0.5 Polyoxyethylene (20E.O.) Sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hardened Castor oil 2.0 Ethanol 10.0 1.0% aqueous sodium hyaluronate solution 5.0 Purified water 80.0

Example 2 (Preparation of a cream) A and B comprising the following components were each heated to 70 ° C., and then A was gradually added to B while stirring, and then slowly stirred. While cooling to 30 ° C., a cream was prepared. (% By weight) A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production example 2 50% ethanol extract of Adatoda basica wood 1.0 B Purified water 47.9 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hydrogenated castor oil 1 Glycerin 5.0 1.0% aqueous solution of sodium hyaluronate 5.0 Methyl parahydroxybenzoate 0.1

Example 3 (Preparation of Cream) Example 2
Was prepared by changing the extract of Preparation Example 2 to the extract of Preparation Example 3.

[Inhibition of Tyrosinase Activity] (Test method) Mcllvaln buffer 0.9m
l, 1.0 ml of 1.66 mM tyrosine solution, 0.1 wt / v% aqueous solution of the above-mentioned preparation (freeze-dried product) (If it is difficult to dissolve, add ethanol and dissolve, then add purified water and evaporate. After removing ethanol, 0.1w
1.0 ml) was placed in a screw vial and heated in a constant temperature water bath at 37 ° C. for 5 minutes or more. Tyrosinase solution (manufactured by Sigma, from mushroom, 914
(Unit / ml) was added, and the solution was kept warm in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner.
In this test, the final concentration of the sample is 0.033%. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} /
B × 100 where A: absorbance of sample specimen B: absorbance of control P: absorbance due to coloring of sample specimen (3 times dilution)

[0018]

[Table 1]

[Hyaluronidase activity inhibition test] (Test method) 0.4% sodium hyaluronate 0.1M
(PH 6.0) After weighing out 6 g of a phosphate buffer solution and leaving it to stand in a constant temperature water bath at 37 ° C. for 5 minutes, a 0.1 wt / v% aqueous solution of the above-mentioned production example (freeze-dried product) was added. After adding and dissolving, purified water was added and evaporated, and ethanol was removed. Then, 1.0 ml of the mixture was adjusted to be 0.1 wt / v%), and the mixture was stirred and 0.01% hyaluronidase (Sigma cattle) Made of testicles, type IS)
1 ml of a 0.1 M (pH 6.0) phosphate buffer was added and the mixture was immediately stirred, and 6 ml of the mixture was placed in an Ostwald viscometer placed in a thermostat bath at 37 ° C. After 1 minute, 5 minutes, 10 minutes, 20 minutes, and 40 minutes, the viscosity was measured. As a control, pure water was added in place of the sample solution, and measurement was performed in the same manner. In this test, the final concentration of each sample is 0.0125% of the concentration of the sample. The results are shown in Table 2 below as indices, with the viscosity after one minute as 100.

[0020]

[Table 2]

[Active Oxygen Suppression Test] There are various methods for measuring the effect of suppressing active oxygen. The following method was used in this study. pH7.8 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml above potassium phosphate buffer with catalase 5 ml 2 mM above potassium phosphate buffer with nitroblue tetrazolium 5 ml 1.8 mM xanthin above phosphorus Potassium acid buffer 17ml 160ml

2.4 ml of the above mixture of reagents and 0.3 ml of specimen
In addition, xanthine oxynase
When conducting the experiment, add 0.3 ml of the above solution so that the absorbance rises to about 0.02 per minute), and immediately measure the absorbance (560 nm). , Check linearity). (Calculation formula) Inhibition rate = {(AB) / A} × 100 where A: change in absorbance per minute when the sample is water B: change in absorbance per minute of sample Several steps were performed to find a 50% active oxygen production inhibiting concentration. A sample was prepared by preparing an aqueous solution of the above-mentioned production example (freeze-dried product) at an appropriate concentration (if it is difficult to dissolve, add ethanol to dissolve it, add purified water, evaporate, remove ethanol, and Concentration). The results of Production Examples 1 and 2 are shown in Table 3 below.

[0023]

[Table 3]

[Antioxidation test] 50 with the following screw cap
ml test tubes. Specimen 5 ml 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml purified water 5 ml This 50 ml test tube with a screw cap is left in a constant temperature bath at 50 ° C. while being shielded from light. Before putting this in a thermostat, after 3 days, 6
After 8 days, the following measurements were taken. Test liquid 0.125m
l, 75% ethanol 12.125 ml, 30% ammonium thiocyanate 0.125 ml, stir for 3 minutes, add 0.02 N ferrous chloride 3.5% HCl aqueous solution 0.125 ml, stir for 3 minutes After standing, wavelength 50
The absorbance was measured at 0 nm. The cell length was 10 mm, and the control cell was one in which the test solution was replaced with water. The results are shown in Table 4 below.
It is shown in FIG.

[0025]

[Table 4]

[0026]

[Table 5]

(Usage test) The face of six women was divided into left and right sides, and the lotion and cream of the example were set on one side, and the lotion and cream of the comparative example were set on the other side, and used at least once a day. After three months,
Evaluation was made on whitening, prevention of rough skin, luster of the skin, and skin swelling. In the comparative examples, various extracts of Adatoba basil in the production examples were replaced with water from the examples (Comparative Examples 1 and 2). In addition, 12 persons were divided into two groups and tested using the samples shown in Table 6 below.

[0028]

[Table 6]

The evaluation was made according to the following evaluation criteria, and the values obtained by summing up the evaluation results are shown in Table 7 below. (Evaluation criteria) Example is very good 3 Example is considerably better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better − 2 Comparative example is much better -3

[0030]

[Table 7]

The above tyrosinase activity inhibition test results (Table 1), hyaluronidase activity inhibition test results (Table 2), active oxygen inhibition test results (Table 3), antioxidant tests (Tables 4 and 5), and use tests (Table 1) As is clear from 7), the cosmetic containing the solvent extract of Adatoda basilica of the present invention suppresses the activity of tyrosinase, the activity of hyaluronidase and the active oxygen, and is effective for rough skin, skin scalp and the like. Was.

[0032]

According to the present invention, there is provided a highly safe cosmetic which has a high whitening effect, suppresses the activity of hyaluronidase, and is effective for rough skin.

────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Masami Nakamura 6-32, Ikegamicho, Toba City, Mie Prefecture (56) References Chemical Abstracts s 54: 105516 (58) Field surveyed (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50 CA (STN)

Claims (1)

(57) [Claims] 1. Adhatoda vasica
A cosmetic comprising a solvent extract of: