KR20120038321A - Cosmetic composition using apios and manufacturing method thereof - Google Patents

Abstract

PURPOSE: A cosmetic composition using apiose and a method for preparing the same are provided to improve skin condition. CONSTITUTION: A cosmetic composition using apiose contains Apios americana Medikus extract as an active ingredient. The extract is prepared from flowers, leaves, and tuber of apiose using low alcohol of 1-4 carbon atoms, polyvalent alcohol, or mixture thereof. A method for preparing the cosmetic composition comprises: a step of extracting apiose; a step of adding 0.01-20 wt% of apiose extract; and a step of formulating in a lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo, or hair rinse.

Description

Cosmetic composition using apios and its preparation method {Cosmetic composition using Apios and manufacturing method

The present invention relates to a cosmetic composition and a method for preparing the same, and more particularly, to a cosmetic composition and a method for producing the same containing an apiose extract excellent in antioxidant and antimicrobial activity.

The skin is the largest organ in the body and is always in direct contact with the external environment and acts as a protective shield to protect the living body from various irritation or dry environments. It is an organ in which new cells are actively produced and destroyed compared to other organs. In addition, it plays a very important role in protecting the body from physical abrasions, preventing the loss of moisture inside the body, protecting it from the ultraviolet rays generated from the sun, and controlling the body temperature.

When the skin is subjected to external stimulation due to various physical factors and external environmental factors, aging such as wrinkle formation, loss of elasticity and keratinization occurs. Skin aging is largely divided into natural aging (or endogenous aging) and external aging. Natural aging is difficult to artificially control because it is influenced by genetic factors, whereas external aging is relatively easy to control because external aging is affected by environmental factors. Do.

Representative external aging factors include ultraviolet rays, and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch.Dermatol., 1992, 128, 347). One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298).

Free radicals are known to cause breakdown of connective tissue formation such as skin collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein function, intercellular energy transfer, and modification of molecules related to metabolism (Lavker RM and Kligman AM, J. Invest.Drmatol., 1988, 90, 325). The fact that free radicals are involved in aging means that antioxidants or various chemical scavengers that inactivate the free radicals can be used to delay the aging process.

Antioxidants such as tocopherol, polyphenols, Coenzyme Q 10, BHT, and BHA are widely used for the purpose of removing such free radicals, but most of them are synthetic products, and thus their use is limited due to safety issues when applied to human skin for a long time. As a result, many studies have been made in anticipation of the development of natural antioxidants of plant origin, which have high antioxidant effects, are guaranteed to be safe for human skin, and are economical.

Apios americana Medikus, on the other hand, is a legume that is vine-like and forms mycorrhiza and tubers in the basement. Apios is native to temperate and subtropical regions of eastern North America, from southeastern Canada to Florida and Texas, USA (Blackmon and Reynolds. 1986; Seabrook and Dionne. 1976). Wild tubers, which are formed in the form of beads in the basement, are used by North American Indians for edible and gangjeong, and are distributed near ancient Indian settlements. In addition, there is a record that early white migrants collected and used it for food, and it was spread to Europe in the 17th century, and an attempt was made to produce crops. However, the yield and long growing period compared to potatoes resulted in full-scale production and use. (Blackmon and Reynolds. 1986; Reynolds et al., 1990; Reynolds, 1995).

The tubers of apios are known to be used directly for food, such as grilling, frying and cooking. Tubers are composed of starch and protein, and the protein content is known to be three times higher than threshing crops using tubers (Ameny et al., 1994; Walter, 1986; Wickremesinhe et al., 1988).

Such apios is known to be cultivated in Korea recently, but studies on apios are still inadequate.

Therefore, the present inventors pay attention to the fact that apios contains various components such as vitamin C while researching to find plant species having skin improvement effect, and the extract extracted from apios has excellent antioxidant and antibacterial activity. The present invention was completed by confirming the fact.

Accordingly, the present invention provides a cosmetic composition and a method for preparing the same, which have an anti-aging, whitening, wrinkle improvement, atopic improvement and a safe use without irritation to the skin by using an apios extract having excellent antioxidant and antibacterial activity. Its purpose is to.

Cosmetic composition using apios of the present invention for achieving the above object is characterized in that it comprises an apios extract extracted from Apios ( Apios americana Medikus) as an active ingredient.

The apios extract is an extract extracted by adding at least one extractant selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof to at least one selected from the flowers, leaves, and tubers of the apios. It is done.

The apios extract is characterized in that the extract extracted from the fermented product at least one selected from the flowers, leaves, tubers of the apios.

And a method for producing a cosmetic composition using the apios of the present invention for achieving the above object comprises the steps of: a) extracting the apios extract from Apios ( Apios americana Medikus); b) Add the apios extract to 0.01 to 20% by weight based on the total weight of the composition, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam It is characterized in that it comprises a; preparing to any one of the formulation selected from hair oil, hair shampoo and hair rinse.

The step a) is characterized in that the apios extract is extracted from the fermented product by mixing the fermentation strain with sugar to at least one selected from the flowers, leaves, tubers of the apios.

As described above, the present invention provides a cosmetic composition that can be used safely without irritation to the skin by containing an active ingredient extracted from apiose which is a natural plant.

In addition, it is expected to be effective in preventing skin aging, whitening, wrinkle improvement, anti-inflammatory, atopy, etc. by strengthening the physiological activity of the skin by the anti-oxidant effect that protects the skin from free radicals and free radicals with excellent antibacterial effect. do.

Hereinafter, a cosmetic composition using apiose according to a preferred embodiment of the present invention and a manufacturing method thereof will be described in detail.

The cosmetic composition of the present invention comprises an apios extract extracted from Apios ( Apios americana Medikus) as an active ingredient.

General components of the tuber of apios, minerals, vitamin constituents are shown in Table 1 below.

General ingredient
(in g / 100g) moisture protein Geology Ash carbohydrate fibrin 53.5 6.1 0.5 1.7 38.2 1.7
Mineral
(in mg / 100g) Ca P Fe Na K - 67 126 02 31 719
vitamin
Vitamin A (R.E) (in mg / 100g) Beta-carotene
(In μg / 100g) Vitamin B1
(in mg / 100g) Vitamin B2 (in mg / 100g) Niacin (in mg / 100g) Vitamin C (in mg / 100g) 2.5 15 0.19 0.05 2.1 21

As such, apios is rich in vitamins A, C, and various minerals and is excellent in antioxidant and moisturizing activity, and is expected to have skin improvement effects such as anti-aging, whitening, wrinkle improvement, anti-inflammatory, and moisturizing.

In the present specification, the apios extract refers to an extract obtained from various organs or parts of the apios as well as the tubers of the apios, and preferably means an extract extracted from at least one selected from flowers, leaves, and tubers.

Apios extract can be extracted in a variety of ways, preferably by mixing 2 to 20 times the weight of the extraction solvent with respect to apios, and then hot water extraction, cold needle or hot needle extraction for 1 to 20 hours at 10 to 150 ℃ Can be used. In addition, in the case of cold or hot extraction can be extracted longer than the time. For example, extraction solvent is added for 10 to 20 days. Further, a reflux cooling extraction method, an ultrasonic extraction method, or the like can be used. In addition to the above-described extraction method, it includes an extract that has undergone a conventional purification process. For example, the active fraction obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value and separation by various chromatography, is also included in the extract.

At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, Butylene glycol, propylene glycol, pentylene glycol, glycerol, etc. can be used as a polyhydric alcohol. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture. In addition, the extract may be obtained in powder form through freeze drying, spray drying, etc.

And another form of the preferred apiose extract is extracted from the fermented fermented apios. Specifically, the fermented product obtained by mixing fermentation with sugar and fermented strain in apios is extracted by centrifugation.

For this purpose, finely chop one or two selected from flowers, leaves, tubers of apios, and then mix them with sugar and put them in a fermentation vessel. At this time, the mixing ratio of 20 to 40 parts by weight per 100 parts by weight of apiose is added to the fermentation vessel. As the sugar, oligosaccharides, lactose, glucose, fructose, sugar, etc. may be used alone or in combination. The fermentation vessel is preferably used onggi. Fermented bacteria are inoculated into the mixture of apios and sugar and stored at 20 to 30 ° C. for 2 to 4 months to ferment.

Fermentation temperature and fermentation period are closely related. If fermentation is carried out at high temperature exceeding 30 ℃, it becomes a good environment for microorganisms to grow regardless of the type, and there is a possibility of deterioration or decay by harmful microorganisms. In addition, since the fermentation broth is not sufficiently fermented at a temperature of less than 20 ° C., a desired fermentation broth cannot be obtained, resulting in a long fermentation period.

Fermentation bacteria are inoculated with 0.01 to 0.03 parts by weight based on 100 parts by weight of the mixture of apiose and sugar. As fermentation bacteria, yeast, yeast or lactic acid bacteria can be used. Preferably Saccharomyces cerevisiae is used as a yeast.

After the fermentation process is completed, the fermented product mixed with liquid and solid is added to a grinder and finely ground and homogenized. Homogenized fermentation is centrifuged to obtain an apios extract.

The cosmetic composition of the present invention preferably contains 0.01 to 20% by weight of the apios extract based on the total weight of the composition. If the apiose extract is less than 0.01% by weight, the antioxidant effect is insignificant, and if it exceeds 20% by weight it is difficult to have an elevated effect compared to the content.

Cosmetic composition of the present invention by adding a variety of well-known ingredients and the like using methods known in the art, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam It may be prepared in any one of the formulations selected from hair oil, hair shampoo and hair rinse.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream or gel, pack, as known components, animal fibers, plant fibers, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide and the like can be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan non-dispersion ester and the like can be used.

Water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester when the formulation of the cosmetic composition of the present invention is a suspension, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tragacanth and the like can be used.

Aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether sulfate when the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent , Alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters and the like can be used.

In addition to the cosmetic composition of the present invention can be used as a conventional known ingredients such as stabilizers, solubilizers, vitamins, pigments and fragrances, can be prepared in any formulation commonly prepared in the art, Can be appropriately selected accordingly.

As an example of the formulation, the lotion may be 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 3.0% by weight of betaine, 3.0% by weight of diethan panthenol, 1.0% by weight of sebum-A, 2.0% by weight of arbutin paste, 2.0% by weight of anenosine, eco gum It may be composed of 0.03% by weight, glycerin 4.0% by weight, collagen 1.0% by weight, apiose extract 5.0% by weight, preservatives (trace), purified water (residual amount).

In addition, as an example of the formulation, the lotion may include 1.0 wt% of carbomer, 2.0 wt% of dimethicone, 5.0 wt% of jojoba oil, 0.5 wt% of provitamin E, 1.0 wt% of olive emulsion wax, 5.0 wt% of arbutin paste, and T-mixture. 1.2 wt%, foam mix 1.0 wt%, batine 1.0 wt%, cetearyl alcohol 1.5 wt%, glycerin 4.0 wt%, flex oil 5.0 wt%, arbutin paste 5.0 wt%, apios extract 5.0 wt%, blending It may be composed of essence oil (trace amount), preservative (trace amount), purified water (residual amount).

In addition, an example of the formulation of the cream is 1.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 1.0% by weight of polysorbate 60, 0.3% by weight of sorbitassesquioleate, 6.0% by weight of cetyloctanoate, Squalane 8.0 wt%, Apricot kernel oil 4.0 wt%, Dimethicone 2.0 wt%, Butylene glycol 4.0 wt%, Glycerin 4.0 wt%, Magnesium aluminum silicate 0.4 wt%, Apiose extract 5.0 wt%, Blending essence oil (Trace amount), preservative (trace amount), and purified water (residual amount).

In addition, as an example of the formulation, the essence is 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, aqueous sodium hyaluronate solution (1%) It may be composed of 0.03% by weight, Diel panthenol 3.0% by weight, glycerin 4.0% by weight, Sebum-A 1.0% by weight, apiose extract 5.0% by weight, blending essence oil (trace), preservative (trace), purified water (residual amount). .

In addition, as an example of the formulation, the foundation may be 2.0% by weight of stearic acid, 0.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 0.5% by weight of polysorbate 60, 0.7% by weight of sorbitan sesquioleate, hydro 2.0% by weight of genelade seed oil, 5.0% by weight of sunflower oil, 6.0% by weight of squalane, 4.0% by weight of cetyloctanoate, 2.0% by weight of cyclomethicone, 5.0% by weight of butylene glycol, 3.0% by weight of glycerin Silicate 0.6%, xanthan gum 0.05%, titanium dioxide 10.0%, talc 3.0%, hydrated ferric oxide 1.3%, ferric oxide 0.3%, ferrus-ferric oxide 0.15%, preservative (trace) , Purified water (remaining amount), may be composed of 5% by weight of apios extract.

In addition, as an example of the formulation, the pack is 0.5 wt% propylene glycol, 1.0 wt% natural liquid, 3.0 wt% polyvinyl alcohol, 3.0 wt% ethanol, 1.0 wt% carbomer, 2.0 wt% collagen, 1.0 wt% eco gum, peptide RF 1.0 wt%, Dipanthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Apios extract 5.0 wt%, Blending essence oil (trace), Preservative (trace), Purified water (Remaining amount) It can be composed of).

In addition, as an example of the formulation, the hair shampoo is 0.5 wt% polyquaternium, 1.0 wt% natural liquid, 3.0 wt% cocamidopropyl betaine, 3.0 wt% betaine, 7.0 wt% CDA, LES 25.0 wt%, cocobetaine 5.0 wt%, Collagen 1.0 wt%, Diel Panthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Apiose extract 5.0 wt%, Blending essence oil (trace), Preservative (trace) , Purified water (residual amount).

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

(Example 1)

The tubers of the apios collected in November were washed thoroughly and then dried naturally to prepare the apios tubers. The prepared apios tuber was finely chopped, water was added 10 times by weight, heated at 100 ° C. for 6 hours, and the solids were filtered to prepare an apios extract.

5 wt% of the prepared extract and 95 wt% of purified water were mixed to prepare a first test sample.

(Example 2)

After finely cutting the apios tuber prepared in Example 1, 90% ethanol was added 5 times in weight ratio, and cooled to room temperature for 6 hours to prepare an apios extract.

5 wt% of the prepared extract and 95 wt% of purified water were mixed to prepare a second test sample.

Example 3

The flowers of apios collected in August were washed thoroughly and then dried naturally to prepare apios flowers. Water was added 10 times by weight to the prepared apios flower, heated at 100 ° C. for 6 hours, and the solids were filtered to prepare an apios extract.

5 wt% of the prepared extract and 95 wt% of purified water were mixed to prepare a third test sample.

Example 4

Apiose extract was prepared by adding 90% ethanol 5 times by weight to the apios flower prepared in Example 3 and cooling for 6 hours at room temperature.

5 wt% of the prepared extract and 95 wt% of purified water were mixed to prepare a fourth test sample.

Example 5

After finely cutting the tuber of the apios prepared in Example 1, 300 g of earth sugar was added to 1 kg of apios tubers, and then 0.02 g of Saccharomyces cerevisiae ( Saccharomyces). cerevisiae , KCTC 7107) and then fermented for 3 months at 25 ℃. The supernatant obtained by centrifugation for 25 minutes at 4000rpm was finely pulverized fermentation product using a grinder was prepared as an apios extract.

5 wt% of the prepared extract and 95 wt% of purified water were mixed to prepare a fifth test sample.

1. Skin test experiment

A skin test was conducted to determine the degree of skin irritation for the first to fifth test samples. For skin tests, a standard patch (IQ chamber, Chemotechnique, Sweden) containing a sample was attached to the back of 20 men and women (mean age 26.5 ± 1.5 years) and removed 24 hours later and read 30 minutes later. 24h single epicutaneous patch test was performed. The degree of response of the skin was determined as 1-4 points according to the ICDRG (International contact dermatitis research group) evaluation criteria for erythema, edema, crust and shallow ulcer, and recorded as a total average value.

Probability of skin irritation is a mean score of 4.5 or more in 4 or more patients (20% of the total subjects). (ICDRG score index 0-1.5: no skin irritation, 1.5-2.5: mild erythema with no skin irritation, 2.5-4.5: mild erythema and edema, further confirmation test required> 4.5: possible skin irritation)

division Skin irritation degree (ICDRG average score index) 1st sample <1.5 2nd test sample <1.5 Third test sample <1.5 4th test sample <1.5 5th test sample <1.5

From the results of Table 2, it was confirmed that all five samples did not show erythema or edema reactions that could cause significant skin irritation in the test subjects. Therefore, when the cosmetic composition of the present invention is applied to the skin, it can be used safely without irritation.

2. Antimicrobial Activity Experiment

The antimicrobial effect on the first to fifth test samples was tested. paper disc method of Gram-positive bacteria Staphylococcus epidermidis by using a (Staphylococcus Epidermis ) was confirmed to inhibit the growth.

The size of the growth zone (clear zone) for each test sample was observed in Table 3 below. As a control, sorbic acid used as a preservative was used.

division Sample 1 2nd test sample Third test sample 4th test sample 5th test sample Control size
(mm) 15.2 15.7 14.5 14.1 18.3 12.3

As shown in Table 3, all of the first to fifth test samples according to the embodiment of the present invention was excellent in antimicrobial activity compared to the existing preservatives. In particular, the antimicrobial activity of the fifth test sample was the highest, which appears to be the result of the increase in the antimicrobial activity of the apios extract useful components produced during fermentation metabolism.

Therefore, the cosmetic composition of the present invention is expected to have an effective antibacterial activity to reduce inflammation of the skin and to remove the itch due to the anti-inflammatory effect on sensitive skin or atopic dermatitis.

3. Antioxidant Activity Test

The results of measuring the radical scavenging activity of the first to fifth test samples are shown in Table 4 below.

The antioxidant donation ability (EDA%) of the samples was measured by modifying Blois' method. 0.5 mL of 0.2 mM DPPH (1,1-diphenyl-2-picryl hydrazyl) was added to 2 mL of the sample solution, stirred for 30 minutes, and the absorbance was measured at 517 nm. The electron donating effect was expressed by the absorbance reduction rate of the sample solution addition and no addition solution. Radical scavenging activity was calculated by the following equation.

% Of radical scavenging activity = (absorbance of 1-sample addition / absorption of no addition) x 100

division Radical scavenging activity 1st sample 59.6 ± 0.35 2nd test sample 60.3 ± 0.45 Third test sample 56.4 ± 0.40 4th test sample 55.8 ± 0.65 5th test sample 65.7 ± 0.50 Control 50.2 ± 0.45

Looking at the results of Table 4, the radical scavenging activity of the first to fifth test samples was higher than the scavenging ability of the BHT (synthetic antioxidant) used as a control 50.2 ± 0.45. And the 5th test sample extracted from the fermentation product had the highest radical scavenging activity.

From this, the present invention is effective in preventing skin aging, whitening, and improving wrinkles by enhancing the physiological activity of the skin by the antioxidant effect of protecting the skin from active oxygen with excellent antibacterial effect.

In the above, the present invention has been described with reference to one embodiment, which is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments are possible.

Therefore, the true scope of protection of the present invention should be defined only by the appended claims.

Claims (5)

Apios ( Apios americana Medikus) cosmetic composition using apios, characterized in that it comprises an apios extract extracted from the active ingredient. The method of claim 1, wherein the apios extract is at least one selected from the flowers, leaves, tubers of the apios at least one extraction solvent selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols or mixtures thereof. Cosmetic composition using apios, characterized in that the extract extracted by adding. According to claim 1, wherein the apios extract is a cosmetic composition using apios, characterized in that the extract extracted from the fermentation of at least one selected from the flowers, leaves, tubers of the apios. a) extracting an apios extract from Apios americana Medikus;
b) Add the apios extract to 0.01 to 20% by weight based on the total weight of the composition, lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam A method for producing a cosmetic composition using apios, comprising the steps of: preparing the hair oil, hair shampoo, and hair rinsing in any one of the formulations selected from the group. The method of claim 4, wherein the step a) comprises extracting the apios extract from the fermented product obtained by mixing fermentation strain with sugar and at least one selected from the flowers, leaves and tubers of the apios. A method for producing a cosmetic composition using an ossu.