KR101855989B1 - Method for producing buckwheat seed extract with increased effective component using water adding solubilizer as extraction solvent and buckwheat seed extract produced by the same - Google Patents

Abstract

The present invention relates to a method for producing a Fagopyrum esculentum seed extract containing increased effective components using water with an added solubilizer as an extraction solvent, and to a Fagopyrum esculentum seed extract containing increased effective components produced by the method. When the water and solubilizer of the present invention is used as the extraction solvent, the amount of the effective components extracted from the Fagopyrum esculentum seed and the efficacy thereof can be maximized, and as organic solvents such as methanol, ethanol, acetone and the like are not used, the Fagopyrum esculentum seed extract has an effect of enhancing human safety and reducing environmental problems, thereby being very useful for related industries using the same.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for preparing buckwheat seed extracts with increased solubility by using water with a solubilizing agent as an extraction solvent, and a buckwheat seed extract produced from the buckwheat seed extract by the same}

The present invention relates to a process for preparing buckwheat seed extract having increased availability by using water containing a solubilizing agent as an extraction solvent and to an buckwheat seed extract having an increased amount of the buoyancy oil produced by the above method.

Solubilization refers to the fact that a substance with a very low solubility in water dissolves beyond its original solubility due to the solubilizing agent. Since solubilization is a thermodynamically stable system unlike emulsification, it can be regarded as an intermediate state between the solubilizing agent and emulsification. The solubilization phenomenon seems to be that the poorly soluble substance is adsorbed and dissolved in the inside of the micelle, and thus the solubilization position is divided into the interior of the micelle, the interior of the micelle and the interface of the pellicle, the pellicle layer, and the surface of the micelle. There is a case where an aqueous solution which is inherently water-soluble is not combined with a membrane and other structural body to become an aqueous solution, and is solubilized by dissociation thereof with a weak denaturant, an alkali treatment, or other treatment. Solubilization techniques using solubilizing agents are used for dissolving non-solubilizing materials, removing oily components from cleaning agents, and oil recovery.

Flavonoid-based compounds have different structural characteristics, such as aglycone and glycoside bonded to sugar in natural state, and they have different structural characteristics such as low molecular weight, middle molecular weight and high molecular weight, , And acetone. This is because the solubility varies depending on the chemical structure. Due to this characteristic, the kind of the substance to be extracted and the physiological activity ability can be changed depending on the solvent.

The most widely used solvents for extracting antioxidants from plants, including flavonoid compounds in buckwheat, are organic solvents such as methanol, ethanol and acetone. This is because it is easy to extract polar substances with high antioxidative effects. However, these organic solvents can damage cells, posing a serious risk to safety. Therefore, if the solvent can be replaced with water, it can contribute to safety as well as environmental problems. In addition, it is considered that extraction with water will not restrict the use of raw materials because there is no side effect in the body.

However, the solubilizing agent is mainly used as an emulsifying agent for foods or cosmetics so far, and studies on the useful components and the physiological activities thereof have not been studied yet. Accordingly, the present invention aims to maximize useful components and efficacy of buckwheat by measuring the physiological activity of buckwheat and bitter buckwheat by adding a solubilizing agent to the buckwheat.

Korean Patent Laid-Open Publication No. 2012-0118752 discloses a cosmetic composition containing bitter buckwheat extract and a method for preparing the same, and Korean Patent Publication No. 2012-0118326 discloses a cosmetic composition containing a bitter buckwheat extract, A method for producing natural antimicrobial agent for food addition and an antimicrobial agent for edible food "has been disclosed. However, as described in the present invention, a method for producing buckwheat seed extract having increased oil content using water containing a solubilizing agent as an extraction solvent, ≪ RTI ID = 0.0 > buckwheat seed extract < / RTI >

SUMMARY OF THE INVENTION The present invention has been made in view of the above needs, and it is an object of the present invention to provide a method for extracting useful materials of buckwheat, including total polyphenols and flavonoid compounds, such as rutin, orientin, isoorientin, , Water with the addition of a solubilizing agent [brij35 (polyoxyethylene lauryl ether), Tween 80 (polyoxyethylene sorbitan monooleate) or Tween 20 (polyoxyethylene sorbitan monolaurate)]. As a result, It was confirmed that the content of the useful ingredient extracted from the buckwheat seeds and the effect thereof were maximized.

In the past, organic solvents such as methanol, ethanol, and acetone were mainly used because it is easy to extract polar substances having a high antioxidative effect. Such organic solvents can damage cells, causing serious safety hazards and environmental problems. I was able to. However, the present invention has been accomplished by confirming that extraction with water containing a solubilizing agent does not have side effects in the body, and that restrictions on the use as a raw material are expected to be reduced.

In order to achieve the above object, the present invention provides a method for producing buckwheat seed extract having an increased oil content, comprising extracting buckwheat seeds with water containing a solubilizing agent as an extraction solvent.

In addition, the present invention provides buckwheat seed extract with increased oil content by the above method.

In addition, the present invention provides a cosmetic composition for whitening skin comprising the buckwheat seed extract with increased oil content as an active ingredient.

In addition, the present invention provides an antioxidative cosmetic composition comprising the buckwheat seed extract with increased oil content as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating skin pigmentation diseases containing the buckwheat seed extract having an increased amount of the above-mentioned component as an active ingredient.

The present invention also provides a composition for extracting useful components of buckwheat seeds containing water with the above-mentioned solubilizing agent added as an active ingredient.

The present invention also provides a method for increasing useful components of buckwheat seed extract comprising extracting buckwheat seeds with water containing a solubilizing agent as an extraction solvent.

The present invention relates to a process for the production of a water-insoluble polyurethane foam by dissolving water in which an additive agent [brij35 (polyoxyethylene lauryl ether), Tween 80 (polyoxyethylene sorbitan monooleate) or Tween 20 (polyoxyethylene sorbitan monolaurate) The present invention relates to a method for extracting useful components from buckwheat seeds and to the use of buckwheat seed extracts having an increased amount of useful components extracted by the method. When water and a solubilizing agent of the present invention are used as extraction solvents, It is possible to maximize the content of the useful ingredient and its effect, and it is very useful for the related industry using the organic solvent because it does not use organic solvent such as methanol, ethanol, acetone, and therefore has an effect of increasing human safety and reducing environmental problems.

FIG. 1 shows the DPPH scavenging ability (%) of the soybean buckwheat and the bitter buckwheat seed extract according to the extraction solvent type. (A) sweet buckwheat, (B) bitter buckwheat
FIG. 2 shows the results of the total polyphenol content (mg / g) of the soybean buckwheat and the bitter buckwheat seed extract according to the type of the extraction solvent. (A) sweet buckwheat, (B) bitter buckwheat
Fig. 3 shows the results of the mushroom tyrosinase-inhibiting activity (%) of soybean buckwheat and bitter buckwheat seed extract according to the type of extraction solvent. (A) sweet buckwheat, (B) bitter buckwheat
FIGS. 4A and 4B are graphs showing the content of five flavonoid compounds (orienting, isoordantene, bitescic, isovichecin, and rutin in the order of peak) including the rutin of short buckwheat seed extract according to the type of extraction solvent Results.
FIGS. 5A and 5B are graphs showing the contents of five flavonoid compounds (orienting, isoordantene, bitescic, isovichecin, and rutin in the order of peak) including the rutin of bitter buckwheat seed extract according to the kind of extraction solvent Results.

In order to accomplish the object of the present invention, the present invention provides a method for producing buckwheat seed extract having increased oil content, comprising extracting buckwheat seeds with water containing a solubilizing agent as an extraction solvent.

In a method according to an embodiment of the present invention, the solubilizing agent is selected from the group consisting of polyoxyethylene (23) lauryl ether, polyoxyethylene (20) sorbitan monooleate or polyoxyethylene May be polyoxyethylene (20) sorbitan monolaurate, preferably polyoxyethylene sorbitan monolaurate, but is not limited thereto.

In the present invention, the polyoxyethylene lauryl ether is the same as brij 35, the polyoxyethylene sorbitan monooleate is the same as Tween 80, and the polyoxyethylene sorbitan monolaurate is the same substance as Tween 20. [

In the method according to one embodiment of the present invention, the solubilizing agent may be added to water at a concentration of 4 to 12 mM, preferably at a concentration of 8 to 12 mM, and most preferably at a concentration of 10 mM But the present invention is not limited thereto.

In a method according to an embodiment of the present invention, the buckwheat may be, but is not limited to, Fagopyrum esculentum Moench or Fagopyrum tataricum Gaertner.

In a method according to one embodiment of the present invention, the useful ingredient may be total polyphenols and flavonoids, and the flavonoids may be, but are not limited to, rutin, orientin, isoorientin, .

In addition, the present invention provides buckwheat seed extract with increased oil content by the above method.

In the buckwheat seed extract according to an embodiment of the present invention, the useful ingredient may be total polyphenol and flavonoid, and the flavonoid may be lutein, orientin, isoorientin, bapticin or isobutyric acid, It does not.

In addition, the present invention provides a cosmetic composition for whitening skin comprising the buckwheat seed extract with increased oil content as an active ingredient.

In the present invention, 'whitening' refers to inhibiting, inhibiting or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, stains, hyperpigmentation after exposure to ultraviolet light, hyperpigmentation after inflammation, aging black spots, brown spots or black spots. The buckwheat seed extract with increased oil content may preferably be a water extract added with 4 to 12 mM of polyoxyethylene lauryl ether, polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monolaurate, More preferably from 8 to 12 mM, and most preferably a water extract to which 10 mM of polyoxyethylene sorbitan monolaurate is added, but is not limited thereto.

In the cosmetic composition for whitening skin according to an embodiment of the present invention, the buckwheat seed extract having an increased amount of the useful component extracted with water containing the solubilizing agent as an extraction solvent may be prepared by dissolving water or methanol not containing a solubilizing agent in an extraction solvent The extracts showed higher DPPH free radical scavenging activity and inhibition of tyrosinase, and higher contents of total polyphenols and rutin, orientin, isoorientin, bitescic and isobutyric acid flavonoids.

The buckwheat seed extract with increased oil content according to an embodiment of the present invention may inhibit tyrosinase activity and inhibit melanin production.

The cosmetic composition for skin whitening according to one embodiment of the present invention can be widely used for cosmetics and cleanser having a skin whitening effect, and is particularly suitable for skin external ointment, cream, softening longevity, nutritional lotion, pack, essence, Shampoo, rinse, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation nutrition But are not limited to, one type of formulation selected from the group consisting of essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The formulation of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a humectant, a fragrance, a fragrance carrier, a protein, a solubilizing agent, a sugar derivative, a sunscreen, a vitamin, a plant extract and the like.

When the cosmetic composition for skin whitening as described above is formulated into pharmaceuticals or cosmetics, it may contain known excipients applicable to the skin acting as a carrier for the active ingredient. When formulating into pharmaceuticals, reference is made to the disclosure of [Remington ' s Pharmaceutical Science, Mack Publishing Company, Easton PA], and in formulation into cosmetics, International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995).

The cosmetic composition for skin whitening according to an embodiment of the present invention may contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, One or more adjuvants selected from water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, But are not limited thereto.

In addition, the present invention provides an antioxidative cosmetic composition comprising the buckwheat seed extract with increased oil content as an active ingredient.

The buckwheat seed extract with increased oil content may preferably be a water extract added with 4 to 12 mM of polyoxyethylene lauryl ether, polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monolaurate, More preferably from 8 to 12 mM, and most preferably a water extract to which 10 mM of polyoxyethylene sorbitan monolaurate is added, but is not limited thereto.

In the cosmetic composition for antioxidation according to an embodiment of the present invention, the buckwheat seed extract having an increased amount of the useful component extracted with the water containing the solubilizing agent as the extraction solvent is obtained by using water or methanol without the solubilizing agent as an extraction solvent DPPH radical scavenging ability is superior to that of buckwheat seed extract.

The present invention also provides a pharmaceutical composition for preventing or treating skin pigmentation diseases containing the buckwheat seed extract having an increased amount of the above-mentioned component as an active ingredient.

In the pharmaceutical composition for the prevention or treatment of skin pigmentation diseases according to an embodiment of the present invention, the pharmaceutical composition for preventing or treating skin pigmentation diseases includes a cream, a gel, a patch, a spray, an ointment, A starch, a starch, a liniment, a pasta or a cataplasma.

The present invention also provides a composition for extracting useful components of buckwheat seeds containing water with an added solubilizing agent as an active ingredient.

The water to which the solubilizing agent is added may preferably be water added with 4 to 12 mM of polyoxyethylene lauryl ether, polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monolaurate, May be water added in an amount of 8 to 12 mM, and most preferably, water added with 10 mM of polyoxyethylene sorbitan monolaurate, but is not limited thereto.

The present invention also provides a method for increasing useful components of buckwheat seed extract comprising extracting buckwheat seeds with water containing a solubilizing agent as an extraction solvent.

The water to which the solubilizing agent for increasing useful ingredients of the buckwheat seed extract is added is preferably 4 to 12 mM of polyoxyethylene lauryl ether, polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monolaurate May be added water extract, more preferably 8 to 12 mM added water extract, most preferably polyoxyethylene sorbitan monolaurate added to 10 mM, but not limited thereto Do not.

In addition, the buckwheat seed extract having an increased amount of the soluble fraction extracted by using the water with the solubilizing agent as the extracting solvent, compared to the buckwheat seed extract extracted with water or methanol without the solubilizing agent, showed DPPH free radical scavenging ability and It has excellent tyrosinase inhibition, high total polyphenol content, and high content of rutin, orientin, isoorientin, bitexin and isobutyric acid flavonoids.

In addition, the present invention provides a skin whitening food composition comprising buckwheat seed extract having an increased amount of the above-mentioned component as an active ingredient.

When the buckwheat seed extract of the present invention is used as a food additive, the extract may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the extract of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, in the production of food or beverage. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. As a specific example, the above-mentioned extract can be used to produce a processed food having good storage properties while utilizing the characteristics of agricultural products, livestock products or aquatic products. Such processed foods include, for example, confectionery, drinks, liquor, fermented foods, canned foods, milk processed foods, meat processed foods, noodles and the like. The sweets include biscuits, pies, breads, candies, jellies, gums, cereals (including dinner utensils such as cereal flakes). Drinks include carbonated beverages, functional ionic beverages, juices (such as apples, pears, grapes, aloes, citrus fruits, peaches, carrots, tomato juices, etc.) and sikhye. The mainstream includes sake, whiskey, shochu, beer, liquor, and fruit wine. Fermented foods include soy sauce, miso, and kochujang. Canned products include canned products (for example, tuna, mackerel, sandwiches, canned fish, etc.), canned products (canned beef, pork, chicken and turkey canned products) and canned products (canned products such as corn, peach and canned pineapple). Milk processed foods include cheese, butter, yogurt and the like. Meat processed foods include pork cutlet, beef cutlet, chicken cutlet, sausage, sweet and sour pork, nuggets, nubucki, and the like. And noodles such as sealed packaging raw noodles. In addition, the composition may be used in retort food, soup and the like.

In addition, functional foods, health foods or health supplements can be produced using the above extract. The functional food, the health food or the health supplement food refers to a food which provides a bio-controlling function including a physiologically active ingredient in addition to the nutritional function, and the extract of the present invention has a skin whitening prevention or improvement effect, Health food supplements and the like.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Materials and methods

1) Experimental material

Buckwheat and bitter buckwheat grown in 2015 in Pyeongchang, Gangwon Province were purchased and stored in a refrigerator at 4 ℃ for the experiment. The solubilizer used in the experiment and the routine used for the HPLC analysis, orientin, isoorientin, bitexin and isovitectin were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

2) Extraction solvent preparation

The extraction solvent was prepared with water, methanol, and water to which the solubilizer was added. Tween 80 (polyoxyethylene sorbitan monooleate) and Tween 20 (polyoxyethylene sorbitan monolaurate) were prepared as the solubilizing agents, and 1 mM, 5 mM and 10 mM each of the solubilizing agents were prepared. And diluted to three concentrations of 10 mM, and used as an extraction solvent.

3) Preparation of extract

1 g of the pulverized sample was taken and 50 mL of the prepared solvent was added. The mixture was extracted with an ultrasound bath at 40 ° C for 30 minutes, centrifuged at 5000 rpm for 5 minutes using a centrifuge (Vision Science, VS-5000N, Was used as a sample.

4) Measurement of DPPH free radical scavenging ability

1 mL of each extract was put into a test tube, 4 mL of 0.15 mM DPPH solution was added thereto, and the mixture was uniformly mixed. The mixture was allowed to stand at room temperature for 30 minutes and absorbance was measured at 517 nm. The DPPH free radical scavenging ability was expressed as a percentage of the absorbance difference between the sample added and the non-added sample.

DPPH free radical scavenging ability (%) = [1- (absorbance of sample addition sphere / absorbance of sample not added sphere) x 100]

5) Total polyphenol content measurement

The Folin-Denis method was slightly modified and measured. 0.2 mL of the extract was placed in a test tube, mixed with 1.8 mL of distilled water, mixed well, and left at room temperature for 3 minutes. 0.4 mL of a saturated solution of 10% Na ₂ CO ₃ was added and mixed, and 1.4 mL of distilled water was added to make a total of 4 mL. The mixture was allowed to stand at room temperature for 1 hour and absorbance was measured at 725 nm. Total polyphenol compounds were determined from standard curves prepared using gallic acid.

6) Measurement of inhibitory activity of tyrosinase

The inhibitory activity of tyrosinase, an enzyme affecting melanin production, was measured by measuring the absorbance of L-dopaquinone (L-dopaquinone), which is produced by reaction with L-DOPA, which is a substrate containing mushroom tyrosinase as an enzyme source. To the prepared test tube, 0.2 mL of 0.1 M phosphate buffer (pH 6.8), 0.2 mL of 5 mM L-DOPA solution and 0.5 mL of sample were mixed and 0.1 mL of tyrosinase (250 U / mL) was added and reacted at 35 ° C. for 2 minutes. Absorbance was measured.

7) HPLC analysis

The analytical sample was dissolved in HPLC water or methanol, filtered through a 0.45 μm membrane filter (Toyo Roshi Kaisha, Japan) and used for analysis. HPLC analysis conditions are as follows.

HPLC analysis conditions Condition Instrument Shimadzu LC system
(CBM-20A, Shimadzu Co, Ltd., Japan) column PrevailC18reversephase 150 x 4.6mm Mobile phase time A: 0.1% formic acid water B: 0.1% formic acid methanol 0 70 30 35 30 70 Oven temperature 40 ℃ Injection volume 10 μl Flow rate 1 ml / min. wavelength 355 nm

8) Statistical processing

Statistical significance was verified at the ρ <0.05 level by Duncan's multiple range test using SPSS (statistical package for social sciences, Version 10.0, Chicago, USA).

Example  One. DPPH  freedom Radical  Measurement of scavenging activity

In the case of buckwheat, 24.19% and 33.87% of activity was observed in water and methanol, respectively (FIG. 1A). The extracts of Brij 35, Tween 80 and Tween 20 showed 51.31%, 52.53% and 53.44% activity at 10 mM concentration, respectively, and the activity was increased in a dose dependent manner. The bitter buckwheat showed 44.93% and 63.21% activity in water and methanol, respectively (Fig. 1B). Brij 35, Tween 80 and Tween 20 showed 73.05%, 75.15% and 76.59% of activity at 10 mM concentration, respectively, and the activity was increased in a concentration-dependent manner.

Example  2. Results of total polyphenol content measurement

In case of buckwheat, the content of water extract was 60.03 mg / g and that of methanol extract was 74.94 mg / g (FIG. 2A). The extracts containing Brij35, tween80, and tween20 showed 142.93 mg / g, 144.92 mg / g and 159.97 mg / g at 10 mM concentration, respectively.

The bitter buckwheat contained 80.22 mg / g and 151.31 mg / g in water and methanol, respectively (Fig. 2B). The extracts containing Brij35, tween80, and tween20 showed 231.03 mg / g, 237.85 mg / g and 244.95 mg / g at 10 mM concentration, respectively.

Example  3. Tyrosinase ( tyrosinase ) Inhibitory activity measurement

In case of buckwheat, 86.02% and 83.23% of activity was observed in water and methanol, respectively (Fig. 3A). The extracts of Brij 35, Tween 80 and Tween 20 showed 79.36%, 78.48% and 77.41% activity at a concentration of 10 mM, respectively, and the inhibitory activity was increased in a concentration-dependent manner. The bitter buckwheat showed 82.98% and 80.05% activity in water and methanol, respectively (Fig. 3B). The extracts of Brij35, Tween 80 and Tween 20 showed 74.93%, 74.63% and 74.20% of activity at 10 mM, respectively, and the inhibitory activity was increased in a concentration-dependent manner.

Example  4. Of the useful substance HPLC  analysis

In case of buckwheat, five flavonoid compounds including rutin were increased compared to the experiment group in which the solubilizing agent was added to water and methanol (Figs. 4A and 4B). The ratios of 9.96 mg / mL in water extracts were increased approximately 2.4 times to 23.05 mg / mL, 23.57 mg / mL and 25.78 mg / mL, respectively, in the experimental group which was added with 10 mM of brij35, tween 80 and tween 20 (Table 2).

The bitter buckwheat also showed a higher content of flavonoid compounds in the experimental group added with the solubilizer than in the experimental group extracted with water and methanol (FIGS. 5A and 5B). The contents of the rutin, which was 584.63 mg / mL in the water extract, were found to be about 1.4 times increased to 815.65 mg / mL, 822.48 mg / mL and 829.58 mg / mL in the extracts containing brij35, tween 80 and tween 20 at a concentration of 10 mM (Table 2).

The content of flavonoids was not significantly different for each of the solubilizing agents, but the content of flavonoids was slightly increased as the concentration was increased. The content of flavonoid compounds, which were not detected in water and methanol extracts, was detected in a small amount in the experimental group to which the solubilizing agent was added.

Changes in the contents of useful ingredients depending on the type of extraction solvent of buckwheat and bitter buckwheat Routine Orientin Isoorientin Beatty Isovichecin water 9.96 + 0.08 a N.D. N.D. 0.49 ± 0.00 a 0.44 ± 0.00 a Methanol 11.95 ± 0.11 b N.D. N.D. 0.58 ± 0.00 b 0.53 ± 0.00 b Brij35
1 mM 12.95 ± 0.15 c 0.66 ± 0.00 a 0.49 ± 0.00 a 0.64 + - 0.01 c 0.57 + - 0.01 c Brij35
5mM 15.28 ± 0.17 e 0.79 + - 0.01 c 0.58 ± 0.00 c 0.76 + 0.02 d 0.67 ± 0.01 d Brij35
10 mM 23.05 ± 0.21 f 1.17 ± 0.05 d 0.87 ± 0.01 e 1.14 ± 0.07 e 1.01 0.04 f Twin 80 1mM 13.21 ± 0.11 cd 0.67 ± 0.01 a 0.50 0.00 a 0.65 ± 0.01 c 0.58 ± 0.00 c Twin 80 5mM 15.80 ± 0.18 e 0.81 0.03 c 0.60 ± 0.01 d 0.78 + 0.02 d 0.70 ± 0.01 e Twin 80 10mM 23.57 ± 0.24 f 1.20 0.04 d 0.89 0.02 e 1.16 ± 0.07 ef 1.04 0.02 f Twin 20 1mM 13.47 ± 0.08 d 0.69 + 0.04 b 0.51 ± 0.01 ab 0.67 + - 0.03 c 0.59 + - 0.01 c Twin 20 5mM 15.93 ± 0.05 e 0.81 0.01 c 0.60 0.02 d 0.79 + 0.02 d 0.70 ± 0.01 e Twin 20 10mM 25.78 + - 0.15 g 1.31 ± 0.03 e 0.98 ± 0.01 f 1.27 ± 0.03f 1.13 + - 0.01 g Routine Orientin Isoorientin Beatty Isovichecin water 584.63 ± 2.74 a N.D. N.D. N.D. N.D. Methanol 735.94 ± 10.19 b N.D. N.D. N.D. N.D. Brij35
1 mM 783.83 + - 6.43 c 0.35 + 0.04 a 0.30 0.02 a 0.52 + 0.03 a 0.74 + 0.05 a Brij35
5mM 803.61 + 12.38 0.48 ± 0.03 b 0.48 ± 0.03 b 0.76 + 0.04 b 0.81 + 0.01 b Brij35
10 mM 815.65 ± 13.15 def 0.59 + - 0.03 c 0.60 0.02 c 0.85 + - 0.03 c 0.95 + - 0.03 c Twin 80 1mM 783.85 + - 11.21 c 0.36 0.02 a 0.31 + 0.01 a 0.53 + 0.01 a 0.76 + 0.01 a Twin 80 5mM 807.98 ± 13.25 0.49 + 0.03 b 0.45 + 0.02 b 0.78 ± 0.03 b 0.83 0.02 b Twin 80 10mM 822.48 +/- 14.11 ef 0.62 ± 0.04 c 0.58 0.02 c 0.89 ± 0.03 cd 1.00 ± 0.05 cd Twin 20 1mM 787.09 ± 11.12 c 0.35 + 0.01 a 0.32 ± 0.01 a 0.54 0.03 a 0.77 + 0.02 a Twin 20 5mM 811.14 ± 13.39 0.50 0.02 b 0.45 + 0.01 b 0.79 + 0.02 b 0.84 0.03 b Twin 20 10mM 829.58 +/- 14.17 eF 0.63 + 0.02 c 0.58 ± 0.01 c 0.90 ± 0.03 cd 1.01 ± 0.04 cd

Claims (12)

Wherein the total polyphenol, the lutein, the orientin, the isotrienethin, the biotecin and the isovitectin synthase, including the step of extracting the buckwheat seeds with water containing 8 to 12 mM polyoxyethylene sorbitan monolaurate as an extraction solvent, A method for producing an increased buckwheat seed extract. delete delete The method according to claim 1, wherein the buckwheat is selected from the group consisting of Fagopyrum esculentum Moench) or bitter buckwheat ( Fagopyrum lt; / RTI &gt; Gaertner). delete delete 4. Buckwheat seed extract with increased total polyphenols, rutin, orientin, isoorientin, bitecin and isovitectin produced by the method of claims 1 or 4. delete delete delete delete Which comprises extracting buckwheat seeds with water containing 8 to 12 mM polyoxyethylene sorbitan monolaurate as an extraction solvent, wherein the total amount of polyphenols, rutin, orientin, isoorientin, And isobutyric acid.

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