KR101914628B1 - Composition for skin anti-wrinkle effect comprising oil from plant as effective component - Google Patents

Abstract

The present invention relates to a composition for improving skin wrinkles containing plant-derived oil as an active ingredient, and more particularly, to a composition for improving skin wrinkles, which comprises a mixture of lavender oil, rosemary oil and citronella seed oil, Since the effect of increasing the synthesis of collagen in cultured artificial skin tissue is remarkable and the effect of improving skin wrinkles is excellent, the composition of the present invention containing a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient, Cosmetics or health functional foods.

Description

[0001] The present invention relates to a composition for improving skin wrinkles containing an oil derived from plants as an active ingredient.

The present invention relates to a composition for improving skin wrinkles containing plant-derived oil as an active ingredient, and more particularly to a cosmetic composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient, ≪ / RTI >

Skin aging refers to the type and intangible changes that appear on the skin as the age goes on. Skin aging can be divided into intrinsic (chronological aging), which is caused by naturally occurring physiological functions of the body as the age increases, and extrinsic photoaging by long-term exposure to ultraviolet light. A clinical feature of endogenous skin aging is that the skin becomes thinner and the elasticity decreases. Histopathological findings include thinning of epidermis and dermis, decreased blood vessels, and decreased number of fibroblasts in the dermis. In addition, the clinical features of photoaging, which is exogenous skin aging, are that the skin becomes rough and unstable, irregular pigmentation occurs, and deep wrinkles increase.

Several causes of wrinkles have been reported. First, when the skin is exposed to excessive ultraviolet rays, collagen decomposition, which is a component of the skin, is accelerated, and the skin may lose elasticity and wrinkles may be formed. In addition, if the skin becomes excessively dry due to excessive temperature change, humidity reduction, wind or the like, skin function as a barrier against the outside may be deteriorated and wrinkles may occur. When the skin is exposed to reactive oxygen species or free radicals, lipid peroxidation is produced by the oxidative action, and skin texture proteins such as collagen may be deformed, resulting in wrinkles. Various cosmetic compositions have been studied to suppress wrinkles caused by external factors and internal factors. In particular, functional cosmetics containing chemical components cause skin irritation, which is not suitable for sensitive skin, and attention is focused on functional cosmetics derived from natural plants that are less irritating to skin and environmentally friendly.

On the other hand, Lavanduia officinalis originates from the Mediterranean coast. Lavender Essential Oil is known to stabilize the mind and body, relieve stress and restore fatigue. It has been used as a bathing agent since the Greco-Roman period, and was also used to incense the laundry in the Middle Ages. Lavender has excellent antimicrobial, anti-inflammatory and antioxidant effects, so it has excellent skin soothing effect and also protects skin from harmful substances.

Rosmarinus officinalis ) is derived from the Latin Rosmarinus, meaning Roses and Marinus, which means "dew on the sea". It is used as a herb since the first century BC in Europe, and it is widely used in cooking, tea, bath salts, lotion, perfume, etc. Rosemary is known to be effective in sterilizing and disinfecting, protecting skin from the outside, and regenerating skin to prevent skin aging.

Yuzu is the fruit of citron, and its skin is bumpy and sour. It contains 3 times more vitamin C than lemon, which is good for cold and skin beauty, and contains a lot of organic acids to prevent aging and fatigue. In addition, vitamin B, carbohydrates and proteins are more common than other citrus fruits, and capillaries are protected by Hesperidin to prevent cerebrovascular disorders and wind. It also excretes wastes accumulated in the body through drainage and excretion. In addition, yuza occupies 10 to 20% by weight of citron, and is rich in active ingredients such as bioflavonoids and remarnoides, and also contains substances having antioxidative properties, so that various functional materials can be developed. However, the flavonoid of yuza is not only mixed with a large amount of lipids but also has flavonoids in the form of glycosides and has a characteristic bitter taste, which makes it difficult to use in foods.

On the other hand, Korean Patent No. 1571323 discloses a cosmetic composition for improving skin comprising an herbal complex extract as an active ingredient, Korean Patent Laid-Open Publication No. 2016-0034707 discloses a cosmetic composition having an aromatherapy effect containing a natural essential oil and a hydrosol Korean Patent Laid-Open Publication No. 2016-0146246 discloses a composition having antioxidative, whitening and wrinkle-reducing effects. However, the composition of the present invention contains a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient The composition for improving skin wrinkles has not been disclosed.

The present invention provides a composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient, wherein the effective ingredient is a lavender oil, a rosemary oil, The present inventors completed the present invention by confirming that the expression of collagen is significantly increased in the artificial skin tissue prepared by three-dimensional culture as compared with the group treated with only citrus seed oil.

In order to solve the above problems, the present invention provides a cosmetic composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient.

The present invention also provides a health functional food composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient.

The lavender oil, rosemary oil, and citronella oil mixture of the present invention has an effect of increasing the synthesis of collagen in the three-dimensional cultured artificial skin tissue to improve the wrinkles of the skin as compared with lavender oil, rosemary oil or citronut oil, The composition of the present invention containing a mixture of rosemary oil and citrus seed oil as an active ingredient can be usefully used as a functional cosmetic for improving skin wrinkles or a material for health functional foods.

FIG. 1 shows the results of cytotoxicity of artificial skin prepared by three-dimensional culture with lavender oil, rosemary oil, citron seed oil and mixtures thereof.
FIG. 2 shows the results of examining the amount of collagen I mRNA expression in artificial skin prepared by three-dimensional culture using lavender oil, rosemary oil, citron seed oil, and mixtures thereof.
FIG. 3 shows the results of confirming the amount of protein expression of collagen I in artificial skin prepared by three-dimensional culture using lavender oil, rosemary oil, citron seed oil and a mixture thereof.
FIG. 4 is a result of examining changes in the expression of skin regeneration and anti-aging factors by irradiating ultraviolet rays on artificial skin prepared by three-dimensional culture and treating with lavender oil, rosemary oil, citron seed oil, and mixtures thereof. Mix oil 1 was treated with 0.0025% (v / v) of a mixture of three oils after UVB irradiation. Oil mixture 2 was treated with 0.05% (v / v) , The oil mixture 3 is a group obtained by treating a mixture of three kinds of oils with 0.01% (v / v) after UVB irradiation, the oil mixture 4 is a group treated with 0.02% (v / v) NC is a negative control group.

The present invention relates to a cosmetic composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient.

The mixture of lavender oil, rosemary oil and citronella oil is preferably a mixture of 0.5 to 1.5 parts of rosemary oil and 0.5 to 1.5 parts of citron oil to 1 part of lavender oil, more preferably 1 part of lavender oil Rosemary oil 1 part skin and citron seed oil 1 part skin, but the present invention is not limited thereto.

In one embodiment of the invention, the composition increases the expression of collagen.

In one embodiment of the present invention, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, for example, as a solution, a suspension, an emulsion, a paste, a gel, a cream, Such as a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage, an oil, a powder foundation, an emulsion foundation, a wax foundation and a spray. It can be made into cosmetic formulations of creams, nourishing creams, eye creams, moisturizing creams, hand creams, essences, nutritional essences, packs, cleansing foams, cleansing waters, cleansing lotions, cleansing creams, body lotions, body cleansers, soaps and powders But is not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

The cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to the above-mentioned effective components, and examples thereof include lipids, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents , Conventional adjuvants such as lipid vesicles, and carriers.

The present invention also relates to a health functional food composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella seed oil as an active ingredient.

The health functional food composition may be formulated into powders, granules, pills, tablets, capsules, candies, syrups and beverages, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient can be appropriately used depending on the purpose of use thereof. Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in the case of long-term ingestion for the purpose of health, the amount may be less than the above range and may be used in an amount exceeding the above range within a range that does not cause any safety problems.

There is no particular limitation on the kind of the health functional food. Examples of the foods to which the health functional food composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, soups, Drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, in the case of a drink, a natural carbohydrate or a flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples. It is to be understood by those skilled in the art that these preparations and examples are merely intended to explain the present invention more specifically and that the scope of the present invention is not limited thereto.

Manufacturing example  1. Production of plant-derived oil

Lavender oil or rosemary oil was prepared by washing lavender or rosemary to remove water, adding each raw material to a commercial oil extractor tank, and operating the extractor for 3 hours and 30 minutes to prepare an oil extract. More specifically, 250 kg of the raw material was put into the extractor tank, and steam steam refined by operating the steam boiler was sucked into the extraction tank and preheated for 1 hour. At this time, decompression was applied to automatically sort the raw materials into heat steam and pressure. When the temperature of the extraction tank reaches 100 ° C, the steam discharge valve is opened and the primary mixed steam is discharged to the outside for about 3 minutes to purify the steam in the extractor. Then, the pressure of the extraction tank is raised to 110 ° C., kgf, the steam extraction valve and the cooling water valve were opened to start the oil separation operation. The oil separation operation was carried out for 2 hours and 30 minutes. When the oil and the hydrosol were separated from the fractionation tank, they received the hydrosol about 3 times and received the hydrosol residue containing the final oil, . In order to obtain pure oil from the separated oil layer, the oil separated first was stored in the freezer for 1 day and only the unfrozen oil was collected.

Yuzu seeds were selected for extracting yuzu seed oil, and the acid and mucus were removed through washing after removing foreign matter. Then, the yuzu seeds were dried to a moisture content of 14% or less, and the dried yuzu seeds were milked through a screw compression method at a low temperature. Milk yielded citron seed oil was passed through a filter to extract high purity citron seed oil.

Lavender oil, rosemary oil and citron seed oil were prepared by mixing each of the obtained oil stock solutions (100%) with 1: 1: 1 poultice.

Example 1. Measurement of cell viability

The cytotoxicity of the oil and oil mixture of Preparation Example 1 was confirmed by MTT assay. In order to overcome this problem, existing monolayer cultures have a disadvantage in that they do not create interactions between cells. Therefore, collagen, which provides a more environmentally similar microenvironment than monolayer culture according to the method of Chonnam National University Dermatology Department Ⅰ, fibroblasts, and keratinocytes (NHAPs) were used in this study. The three - dimensional artificial skin model was prepared by using a three - dimensional culture system.

As a result, as shown in Fig. 1, the oil mixture, rosemary oil and citron seed oil according to the present invention showed almost no cytotoxicity even when treated at a concentration of 0.08% (v / v). However, when lavender oil alone was treated, it was confirmed that toxicity was observed even at a concentration of 0.02% (v / v).

Example  2. Identification of skin regeneration efficacy by plant-derived oil

The three-dimensionally cultured artificial skin was treated with 0.01% (v / v) concentration of lavender oil, rosemary oil, citron seed oil and a mixture thereof of Production Example 1 at a concentration not showing cytotoxicity, , The amount of collagen I synthesized was confirmed at the level of mRNA and protein.

As a result, as shown in Fig. 2, mRNA expression was increased in each individual oil (lavender, rosemary or yuza seed) and a mixture thereof when compared to the control (control), and in particular, lavender oil, rosemary oil, It was confirmed that the expression of the three kinds of oil mixture was remarkably increased when compared with the group treated with the seed oil alone.

In addition, as shown in Fig. 3, the expression of protein at the protein level that performs the actual function using the ELISA was confirmed. As a result, the expression of the protein of collagen I was decreased in rosemary oil and yuza seed oil, The expression of the three mixture of oils was increased, especially the mixture of the three oils showed a markedly enhanced expression compared to the lavender oil.

The above results confirmed the synergistic effect of the mixture of lavender oil, rosemary oil and citronella oil.

Example  3. To check the regenerating effect of skin damaged by ultraviolet irradiation when treating plant oil mixture

The artificial skin cultured in three dimensions was irradiated with ultraviolet rays (UVB 10 mJ / cm 2) to form an aging environment, and then the effect of the lavender oil, rosemary oil and citronella oil mixture of the present invention on skin was examined.

As a result, as shown in Table 1, it was confirmed that mRNA expression of skin regeneration and anti-aging factors changes in artificial skin damaged by ultraviolet rays in proportion to the treatment concentration of the mixture.

Oil mixture treatment concentration (%, v / v) Increase in collagen I compared to control (UVB irradiation) (%) Increase in collagen II (%) versus control (UVB irradiation) Increase in TGF-β1 (%) versus control (UVB irradiation) Reduction of MMP-1 compared to control (UVB irradiation) (%) Reduction of caveolin-1 compared to control (UVB irradiation) (%) 0.0025 20.9 17.7 20.7 4.3 2.7 0.005 44.4 35.3 30.1 7.9 13.2 0.01 58.3 55.3 39.7 14.4 21.9 0.02 75.0 66.4 59.2 20.2 34.6

As shown in FIG. 4, the protein expression of skin regeneration factor, aging factor and signal transduction factor was examined. As a result, the expression of collagen and TGF-β1 decreased by ultraviolet rays, And the expression of MMP-1 and caveolin-1, which had been increased by the addition of the antioxidant, was reduced to the normal artificial skin level.

It was confirmed that the mixture of lavender oil, rosemary oil and citron seed oil was effective in the regeneration and antiaging of skin damaged by ultraviolet rays.

Claims (5)

Lavender oil 1 part Rosemary oil 0.5 - 1.5 parts Skin and citronellulose 0.5 - 1.5 parts Cosmetic composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil mixed with skin as an active ingredient. delete The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a skin, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, The cosmetic composition for improving skin wrinkles according to any one of claims 1 to 3, wherein the composition is one selected from the group consisting of an essence, a pack, a cleansing foam, a cleansing water, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a soap and a powder. Lavender oil 1 part Rosemary oil 0.5 to 1.5 parts Skin and citronella oil 0.5 to 1.5 part A health functional food composition for improving skin wrinkles containing a mixture of lavender oil, rosemary oil and citronella oil as an active ingredient . The health functional food composition for improving skin wrinkles according to claim 4, wherein the composition is prepared from one of powder, granule, ring, tablet, capsule, candy, syrup and beverage.

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