KR102097302B1 - Composition for promoting hair growth or preventing hair loss comprising oil from plant as effective component - Google Patents

Abstract

The present invention relates to a composition for promoting hair growth or preventing hair loss, which contains a plant-derived oil as an active ingredient. More specifically, the mixture of lavender oil, rosemary oil, and citron seed oil of the present invention increases the expression of hair growth factors and antioxidant enzymes in human dermal papilla cells using three-dimensional culture compared to individual substances, and hair growth factors in hair loss cell models. Since it has an effect of increasing expression, the composition of the present invention including it as an active ingredient may be useful as a cosmetic or health functional food for preventing or improving hair loss, as an external preparation for skin for preventing or treating hair loss.

Description

Composition for promoting hair growth or preventing hair loss comprising oil from plant as effective component

The present invention relates to a composition for promoting hair growth or preventing hair loss containing an oil derived from a plant as an active ingredient, and more specifically, for promoting hair growth or preventing hair loss containing a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient. It relates to a composition.

The human body has about 100,000 to 150,000 hairs, and each hair has different hair cycles, repeats growth, anagen, catagen, and telogen. About 15% of the total hair is placed in the resting period and the degenerative phase, so on average, 50 to 100 hairs per day are normally removed and reproduced. This hair growth cycle is repeated about 3 to 6 years in cycles. Generally, alopecia means that the period corresponding to the anagen of the hair cycle is shortened due to various causes, so that the ratio of degenerative and resting hair is relatively high, so that the number of hair fall-outs is higher than the average value. Is a genetic androgen hormone action, nutritional disorders due to local blood flow disorders, autonomic nervous system disorders, autoimmune disorders, endocrine disorders, but until now, the definite cause of hair loss has not been identified, and the above-mentioned causes occur in combination It is said to be.

The root part of the hair root where hair grows is called the hair bulb, and the recessed part of the lower part is called the breast nipple. Breast nipples are the most important part of the development of hair, and the nipples have blood vessels or nerves, and they supply nutrients to the hair cells immediately above, thereby promoting hair growth. The number of these nipples is determined at birth, and as the number of nipples disappears and the number decreases, the number of hairs decreases.

Recently, the population causing abnormal hair loss is gradually increasing due to changes in eating habits, lifestyle changes, and excessive stress caused by the social environment. In particular, there is an increase in the number of young and mature hair loss women. On the other hand, hair loss, which refers to a condition in which hair or hair is less or less than normal due to some cause, is divided into general hair loss phenomenon (hair loss hair loss, telogen effluvium) in which hair falls out of a resting state and anagen effluvium, which is an abnormal hair loss phenomenon. You can do it, but in general, alopecia refers to the latter. Types of alopecia include alopecia areata, early alopecia, androgene alopecia in women, syphilis alopecia, and male pattern baldness.

The most representative anti-hair loss agent developed for hair loss is finasteride, which is a drug that inhibits the activity of 5-α-reductase, which produces dihydrotestosterone, which is a direct hair loss-causing agent. However, in the case of the finasteride preparation, it may cause fetal malformations in pregnant women, and side effects such as decreased libido, erectile dysfunction, and ejaculation disorders have been reported during long-term administration. In addition, the widely used minoxidil (minoxidil) -containing hair growth agent is also a drug approved by the FDA as a general drug, but it cannot be administered to women and has disadvantages such as skin irritation, itching, redness, and worsening of hair loss. Therefore, there is a need to develop a material that is safe for both men and women and has no side effects and has hair loss prevention and hair growth effects.

Meanwhile, Lavanduia officinalis is native to the Mediterranean coast. Lavender essential oil is known to stabilize the mind and body, relieve stress and relieve fatigue, and has been used as a bathing agent since the Greek-Roman era, and was used to smell laundry in the Middle Ages. Lavender has excellent antibacterial, anti-inflammatory, and antioxidant effects, so it has excellent skin soothing effects and also protects the skin from external harmful substances.

Rosemary (Rosmarinus officinalis ) is derived from the Latin word "Rosmarinus", namely Rose (Ros: dew) and Marinus (Marinus). Stems, leaves, and flowers are all used. In Europe, they have been used as herbs since the 1st century BC and are widely used in cooking, tea, bathing agents, lotion, and fragrances. Rosemary has excellent sterilization and disinfecting properties, and is known to protect the skin from the outside and regenerate the skin to prevent skin aging.

Citron is the fruit of the citron tree, characterized by uneven skin and sour taste. As the main ingredient, vitamin C contains 3 times more than lemon, which is good for cold and skin beauty, and contains a lot of organic acids that prevent aging and fatigue. In addition, vitamin B, sugar, and protein are more than other citrus fruits, and hesperidin, which protects capillaries, prevents cerebrovascular disorders and wind. In addition, it excretes wastes accumulated in the body by excretion and excretion.

In addition, citron seeds occupy 10 to 20% by weight of citron weight, and are rich in active ingredients such as bioflavonoids and limonoids, and contain substances with antioxidant properties, so that they can be developed into various functional materials. However, since the flavonoids of Yuja seeds are not only mixed with a large amount of lipids, but also have flavonoids in the form of glycosides, and thus have a unique bitter taste, it is difficult to use in foods. .

On the other hand, Korean Patent Publication No. 2003-0075283 discloses a non-polluting citron shampoo composition and a method for manufacturing the same, but a composition for promoting hair growth or preventing hair loss containing the mixture of lavender oil, rosemary oil and citron seed oil of the present invention as an active ingredient Has not yet been disclosed.

The present invention was derived by the above-described needs, and when the active ingredient of the present invention, a mixture of lavender oil, rosemary oil and citron seed oil, was treated in 3D cultured human dermal papilla cells (hDPC), The present invention was completed by confirming that the expression of hair growth factors and antioxidant-related factors increased and the expression of hair growth factors increased in the hair loss cell model compared to the case where each of these individual oils was treated.

In order to solve the above problems, the present invention provides a cosmetic composition for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient.

In addition, the present invention provides a skin external preparation for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient.

In addition, the present invention provides a health functional food composition for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil, and citron seed oil as an active ingredient.

The present invention relates to a composition for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient, wherein the mixture of lavender oil, rosemary oil and citron seed oil is lavender oil, rosemary oil or citron seed oil Increases the expression of IGF and VEGF, hair growth factors, in human dermal papilla cells (hDPC) cultured in 3D compared to alone, and antioxidant-related factors HO-1, NQO-1, GSTpi, Nrf-2 Since the effect of increasing the expression and the effect of increasing the expression of IGF and VEGF in the hair loss cell model is excellent, it can be usefully used as a cosmetic, skin external preparation or health functional food composition for promoting hair growth or preventing hair loss.

1 is a result of confirming the expression of hair growth factors (IGF and VEGF) after treating the oil of the present invention to human dermal papilla cells. *, ** means that the gene expression of the oil mixture treatment group was significantly increased compared to the lavender oil alone treatment group, which was the most effective among the treatment groups, * is p <0.05, ** is p <0.01 .
2 is a result of confirming the expression of antioxidant enzymes (HO-1, NQO-1 and GSTpi) after treating the oil of the present invention to human dermal papilla cells. * Means that the expression of antioxidant enzymes (HO-1 and NQO-1) in the oil mixture treatment group was significantly increased compared to the lavender oil alone treatment group, which was the most effective among the treatment groups, and p <0.05.
3 is a result of confirming the expression of Nrf-2 after treating the oil of the present invention to human dermal papilla cells.
4 is a result of confirming the expression of hair growth factors (IGF and VEGF) after treating the oil of the present invention in a hair loss cell model established by treating INF-γ. ** means that the gene expression of the oil mixture treatment group was significantly increased compared to the oil alone treatment group, and p <0.01.

The present invention relates to a cosmetic composition for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient.

The lavender oil, rosemary oil and citron seed oil mixture is preferably mixed with 0.5 to 1.5 parts by volume of rosemary oil and 0.5 to 1.5 parts by volume of citron seed oil with respect to 1 part by volume of lavender oil, more preferably 1 part of lavender oil It is a mixture of 1 part by volume of rosemary oil and 1 part by volume of citron seed oil for the skin, but is not limited thereto.

The mixture of lavender oil, rosemary oil, and citron seed oil of the present invention may be to promote growth of dermal papilla cells, but is not limited thereto.

In addition, the cosmetic composition is hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, Hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair drying agent, hair preservation treatment agent, hair dye, hair wave agent, hair decolorant, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair It may be prepared in the form of a mousse or hair spray, but is not limited thereto.

In addition, the cosmetic composition is a fatty acid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent (foaming) in addition to the lavender oil, rosemary oil, and citron seed oil mixture of the present invention agent), fragrance, surfactant, water, ionic or nonionic emulsifier, filler, metal ion blocker and chelating agent, preservative, vitamin, blocker, wetting agent, essential oil, dye, pigment, hydrophilic or lipophilic active agent, It may contain adjuvants commonly used in the cosmetic field, such as lipid vesicles or any other ingredients commonly used in cosmetics.

In addition, the present invention relates to an external preparation for preventing hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient.

The external skin preparation for preventing hair loss or promoting hair growth of the present invention may be prepared in the form of a cream, gel, patch, ointment, warning agent, lotion, liquid, emulsion, powder or spray, but is not limited thereto.

When the formulation of the present invention is a cream or a gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane / Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a liquid or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

In addition, the present invention relates to a health functional food composition for promoting hair growth or preventing hair loss, which contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient.

The health functional food composition may be prepared as a powder, granule, pill, tablet, capsule, candy, syrup, or beverage formulation, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient can be appropriately used depending on the purpose of use (prevention or improvement). Generally, the health functional food composition of the present invention in the manufacture of a food or beverage is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There are no particular restrictions on the type of the health functional food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum products, dairy products including ice cream, various soups, beverages, tea Drinks, alcoholic beverages, and vitamin complexes are included, and include all healthy foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients that are commonly added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when manufactured as a drink agent, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg. , Xylitol, sorbitol, erythritol, and the like). The flavoring agent may be a natural flavoring agent (eg, tau martin, stevia extract, etc.) and a synthetic flavoring agent (eg, saccharin, aspartame, etc.).

Various nutritional supplements, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonic acid, in addition to the above-mentioned health functional food composition It may further contain a carbonizing agent used in beverages. The proportion of the components to be added is not particularly important, but it is generally selected from the range of 0.01 to 0.1 parts by weight with respect to 100 parts by weight of the health functional food composition of the present invention.

Hereinafter, the present invention will be described in more detail by using manufacturing examples and examples. It is obvious to those skilled in the art that these production examples and examples are only intended to illustrate the present invention more specifically and that the scope of the present invention is not limited by them.

Production Example 1. Preparation of oil derived from plants

In order to prepare lavender oil or rosemary oil, water was removed by washing the lavender or rosemary, and raw materials were respectively added to a commercial oil extractor tank and the extractor was operated for 3 hours and 30 minutes to prepare an oil extract. More specifically, 250 kg of raw materials were put in the extractor tank, and the steam steam purified by the operation of the steam boiler was sucked into the extraction tank, and preheated for 1 hour. At this time, the pressure was reduced to automatically sort the raw materials into heat vapor and pressure. When the temperature of the extraction tank reaches 100 ℃, the steam discharge valve is opened to discharge the primary mixed steam out for about 3 minutes to purify the steam in the extractor. Then, after increasing the pressure, the temperature of the extraction tank reaches 110 ℃ and the pressure is 1.5. When it took kgf, the steam extraction valve and the coolant valve were opened to start the refinery separation operation. The essential oil separation operation is performed for 2 hours and 30 minutes, and when the oil and hydrosol are separated from the sorting tank, the hydrosol is received about 3 times, and the hydrosol residue containing the final oil is received and the hydrosol and oil are separated through a separator. Was separated. In order to obtain pure oil from the separated oil layer, the primary separated oil was stored in the freezer for one day to collect only unfrozen oil.

To extract the citron seed oil, the citron seed was screened, and after removing foreign substances, the acid and mucus were removed by washing. Thereafter, the citron seeds were dried to a water content of 14% or less, and the dried citron seeds were milked in a low temperature state through a screw compression method. The milked citron seed oil was passed through a filter to extract high-purity citron seed oil.

A mixture of lavender oil, rosemary oil and citron seed oil was prepared by mixing 1 volume part of rosemary oil and 1 volume part of citron seed oil per volume part of lavender oil.

Example  1. Confirm the effect of plant-derived oil on the expression of hair growth factors

In order to confirm the effect of the lavender oil, rosemary oil, citron seed oil, or a mixture thereof in Preparation Example 1 on the expression of hair growth factors, using human dermal papilla cells and type 1 collagen according to the method of the Department of Dermatology, Chonnam National University The experiment was conducted on a three-dimensional model prepared with a three-dimensional cell culture system.

When 0.005% (v / v) of oil (lavender oil, rosemary oil, citron seed oil or a mixture thereof) that does not show cytotoxicity based on 100% of the cell culture medium is treated with 3-dimensional cultured papilloma cells, As shown in 1, it was confirmed that the expressions of IGF and VEGF which have a positive effect on hair growth were increased, and compared to the treatment with lavender oil, rosemary oil, and citron seed oil alone, IGF was treated with a mixture of the above three oils. And it was confirmed that the expression of VEGF significantly increased.

Therefore, a mixture of lavender oil, rosemary oil, and citron seed oil may have a more positive effect on hair growth compared to each individual oil.

Example  2. Confirmation of antioxidant enzyme expression

The oil of Preparation Example 1 was treated on the three-dimensional cultured dermal papilla cells to confirm the expression change of antioxidant enzymes (HO-1, NQO-1 and GSTpi) through RT-PCR. As a result, as shown in Figure 2, it was confirmed that the expression of the antioxidant enzyme increases during the treatment of the oil of Preparation Example 1, compared to the lavender oil, rosemary oil, citron seed oil alone compared to the mixture of the three oils When treated, the expression of HO-1, NQO-1 and GSTpi was further increased.

Example  3. Confirmation of the mechanism of antioxidant enzyme expression

When the oil of Preparation Example 1 was treated, expression of Nrf-2, a key transcription factor that induces antioxidant, was confirmed to confirm a mechanism for increasing the expression of antioxidant enzymes. As a result, as shown in Figure 3, when the oil of Preparation Example 1 was treated with 3D cultured dermal papilla cells, the expression of Nrf-2 increased compared to the negative control (Control), and lavender oil, rosemary oil, and citron seed oil It was confirmed that the expression was further increased when the mixture of the three oils was treated compared to the treatment alone.

Example  4. Breast nipples  Determining the effect of plant-derived oil on hair growth factor expression in alopecia cell model

After treatment of INF-γ in human cultured human dermal papilla cells (hDPC) to create a hair loss cell model, the oil of Preparation Example 1 was treated to confirm expression of hair growth factors. As a result, as shown in FIG. 4, the expression of IGF and VEGF was reduced in the papillary cells by INF-γ treatment, and the lavender oil, rosemary oil, citron seed oil, or a mixture of the three oils of Preparation Example 1 was treated. In case, the reduced expression levels of IGF and VEGF were increased again. In addition, it was confirmed that the expression was further increased when the mixture of the three oils was treated compared to the treatment of lavender oil, rosemary oil, and citron seed oil alone. Therefore, the mixture of three kinds of lavender oil, rosemary oil, and citron seed oil of the present invention can be usefully used to prevent hair loss.

Claims (8)

Contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient mixed with 1 part by volume of rosemary oil and 1 part by volume of citron seed oil with respect to 1 part by volume of lavender oil, and promotes hair growth to promote growth of dermal papilla cells, or Cosmetic composition for preventing hair loss. delete delete According to claim 1, wherein the composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, Hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair drying agent, hair preservation treatment agent, hair dye, hair wave agent, hair decolorant, hair gel, hair glaze, hair dresser, hair lacquer, hair moisture A cosmetic composition for promoting hair growth or preventing hair loss, characterized in that it is produced in a formulation of a riser, hair mousse, or hair spray, and promotes growth of hair papilla cells. Contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient mixed with 1 part by volume of rosemary oil and 1 part by volume of citron seed oil with respect to 1 part by volume of lavender oil, and promotes hair growth to promote growth of dermal papilla cells, or Skin external preparation for hair loss prevention. The method of claim 5, wherein the external preparation for skin is prepared in the form of a cream, gel, patch, ointment, warning, lotion, liquid, emulsion, powder or spray, and promotes growth of dermal papilla cells. An external preparation for skin for promoting hair growth or preventing hair loss. Contains a mixture of lavender oil, rosemary oil and citron seed oil as an active ingredient mixed with 1 part by volume of rosemary oil and 1 part by volume of citron seed oil with respect to 1 part by volume of lavender oil, and promotes hair growth to promote growth of dermal papilla cells, or Health functional food composition for preventing hair loss. According to claim 7, The composition is powder, granules, pills, tablets, capsules, candy, syrup, and any one selected from the formulation selected from the drink, hair growth promoting or hair loss characterized in that it promotes the growth of papillary cells. Preventive health functional food composition.

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