KR102205789B1 - Extermal composition for preventing hair loss containing extracts derived from natural products and method for manufacturing the same - Google Patents

Abstract

The present invention relates to an external preparation composition for preventing hair loss containing an extract derived from a natural product, and a manufacturing method thereof. Specifically, a composition of the present invention contains an extract derived from a natural product, thereby exhibiting an excellent moisturizing effect when applied to the skin to create an optimal environment to promote scalp growth, and simultaneously exhibiting complex effects of lowering the heat of the scalp, promoting the differentiation of dermal papilla cells, and thickening the hair.

Description

A composition for external use for preventing hair loss, including extracts derived from natural products, and a manufacturing method thereof {EXTERMAL COMPOSITION FOR PREVENTING HAIR LOSS CONTAINING EXTRACTS DERIVED FROM NATURAL PRODUCTS AND METHOD FOR MANUFACTURING THE SAME}

The present invention relates to a composition for external application for preventing hair loss comprising an extract derived from a natural product and a method for preparing the same.

Human heads usually have around 100,000 hairs on average, and they repeat their formation and extinction while undergoing periodic changes in the anagen, catagen, telogen, and depilatory periods. Hair is composed of 90% keratin composed of 18 kinds of amino acids and about 10% moisture as a basic component, and the constituent elements are carbon, oxygen, nitrogen, hydrogen, and sulfur.

Human hair is formed from hair follicles. Hair follicles contain dermal nipples, in which small blood vessels are distributed to supply nutrients necessary for hair growth, and sebaceous glands that secrete sebum to protect hair are distributed on the upper side of the dermal papilla.

The breast nipple is a headquarter that regulates hair growth, and is the site where male hormones work in male pattern hair loss. The area where cell division occurs according to the regulation in the dermal nipple is the mosquito and the area that generates hair.

The most well-known hair loss factor in men is the effects of abnormal hormones. During puberty, sex hormones are actively secreted and secondary sexual characteristics appear. These changes are caused by the sex hormones androgens (male hormones) and estrogens (female hormones). Androgens develop hair from the eyebrows to the bottom, and estrogen primarily promotes the growth of the hair. In males, baldness is due to excessive secretion of androgens, which inhibits the action of estrogen.

On the other hand, in modern society, in particular, various pollutants, physical stimulation factors, excessive smoking, alcohol consumption, and stress cause oxidative stress in life.

According to the results of recent research, excessive oxidative stress can convert the growing hair into a resting phase, and variously worsen the hair condition, leading to hair loss, and the type 2 beta-corticosteroid secreting hormone receptor around the hair follicle. It is known that it can cause alopecia areata by activating.

Currently, various studies for preventing hair loss and hair growth are being actively conducted, and particularly recently, studies on hair loss prevention and hair growth using herbal herbal medicines are active.

Accordingly, the present inventors attempted to invent a composition that is harmless to the human body and exhibits excellent anti-hair loss effect by including extracts derived from various natural substances.

Korean Patent Publication No. 10-2011-0104196

One aspect of the present invention is a thief's cane root extract, pepper fruit extract, goji berry extract, green tea extract, angelica root extract, beetroot extract, mulberry fruit extract, mulberry root extract, giant pine leaf extract, chichi root extract, hawthorn root extract It provides a composition for preventing hair loss and promoting hair growth, comprising one or more selected from the group consisting of lavender extract, bergamot leaf extract, peppermint leaf extract, freesia extract, matricaria flower extract and rosemary leaf extract.

Another aspect of the present invention provides a composition for external application for skin for preventing hair loss and promoting hair growth, including the composition for preventing hair loss and promoting hair growth.

Another aspect of the present invention provides a cosmetic composition comprising the composition for preventing hair loss and promoting hair growth.

One aspect of the present invention for solving the above problem is a thief's cane root extract, pepper fruit extract, goji berry extract, green tea extract, angelica root extract, copper root extract, mulberry fruit extract, mulberry root extract, giant cypress leaf extract, A composition for preventing hair loss and promoting hair growth, comprising at least one selected from the group consisting of chichi root extract, hawthorn root extract, lavender extract, bergamot leaf extract, peppermint leaf extract, freesia extract, matricaria flower extract, and rosemary leaf extract to provide.

Another aspect of the present invention provides a composition for preventing hair loss and promoting hair growth, comprising a fermented extract obtained by treating the extract with probiotics.

In the present invention, the “thief's stick ( Sophora flavescens AIT )” is a perennial herbaceous plant belonging to the legume family, and is also called gosam. The name Gosam was given because the taste was very bitter, and the name'thief's wand' was given because the shape of the root was ugly. Matrine is contained as a medicinal ingredient, and antipyretic and diuretic effects were recognized in animal experiments. Since it has a geonwi effect, it can be cured by taking a decoction with gastroenteritis, enteritis, and bacterial dysentery such as Mokhyang (木香) and licorice.

In addition, it is also effective for purulent and eczema of the skin, and it is treated by washing the affected area by decoction even when the itching is severe in the woman's vulva. It is known to treat the skin by adding an ointment to the affected area by compounding Gobaekban (枯白礬) and the like.

In the present invention, the term “gojija” refers to the fruit of Lycium chinense , and the fruit is used as an antipyretic and tonic in herbal medicine, and it is also eaten with a decoction or liquor. Among the ingredients contained in wolfberry, betaine prevents fat from getting tangled in the liver and has the effect of healing fatty liver. It helps to release or detoxify toxic substances, and if you consume it consistently, it not only prevents aging, but also improves fat metabolism, which helps in an effective short-term diet. In addition, because it warms the body, it is known to have various effects such as fatty liver removal, nervous breakdown, and chronic liver disease.

In the present invention, the "Cham Angelica gigas " is a perennial plant belonging to the Ranunculus family and is distributed in Korea, China, and Japan. The height is about 1-2m, the whole stem is purple and has a lot of scent. Flowers are purple, and bloom in groups in a double-mounted inflorescence in August-September. Fruit is oval and wide wings hang on the edge. The root is usually called angelica and is used in herbal medicine for sedatives, tonics, hemorrhoids, and anemia.

In the present invention, "Angelica dahurica" is a perennial plant belonging to the umbel family and is distributed in Northeast Asia and mainly grows at the edges of mountains and valleys. The height is about 1~1.5m, and there are a lot of beard roots on the fat rootstock. The leaves are split into several branches, elliptical, and 5-10cm long. In oriental medicine, the root is called Baekji and is used as a sedative, pain reliever, and hemostatic agent.

In the present invention, the “chichi ( Lithospermum erythrorhizon )” is a perennial plant of the Chichia family and is distributed throughout Korea. The root is used as a dye or medicine. For medicinal purposes, it has dry resistance and insect resistance, so it is used for dry stomach, detoxification, burns, and frostbite. When a dyed cloth is placed on the skin, window poison is removed and the mass is removed. It is known that it does not occur.

In the present invention, the " Pleuropterus multiflorus " is a perennial vine plant grown as a medicinal herb native to China. It is known that the efficacy of Hasuoh is to help the liver and kidneys, such as boils and insomnia, and to blacken hair.

In the present invention, the "Lavender ( Lavandula Angustifolia )" is known to have psychological effects such as sedation, anxiety reduction, antiemetic, antidepressant, sedation, and physiological effects of sedation, anxiety reduction, antiemetic, antidepressant, sedation, There is no known effect related to hair loss treatment.

In the present invention, the "bergamot ( citrus aurantium bergamia )" is known to have psychological effects such as mental lift, tension relief, stress relief, and physiological effects such as blood circulation, digestion promotion, stress-related indigestion and loss of appetite, There is no known effect related to hair loss treatment.

In the present invention, the "peppermint ( Mentha arvensis )" is known to have physiological effects such as mental enhancement, mental strengthening and antiemetic, gastrointestinal relief, anticonvulsant, expectorant, neuralgia relief, lymphatic tissue vitality, etc. No treatment-related effects are known.

In the present invention, the " Freesia refracta " is a perennial plant of the iris family, and is used in the manufacture of cosmetics such as hand creams, shampoos, and skin cleansers because of its good scent. In ancient times, it is known to have been used for depression or uterine reinforcement. In addition, the root of freesia can cause skin irritation and allergies, so people with sensitive skin should be careful when using it.

In the present invention, the " Matricaria chamomilla " is otherwise known as German chamomile, and is an annual plant of the Asteraceae family. The flower, stem, and leaf extracts of the Matricaria plant contain flavone derivatives and coumarin, which are known to have a skin soothing effect and help skin elasticity.

In the present invention, the " Rosmarinus Officinalis " is known to have psychological effects such as antidepressant and physiological effects such as blood circulation, guttural, bile secretion promotion, rejuvenation, and digestion promotion, but the effects related to hair loss treatment Unknown.

The "extract" refers to a solvent in which the active ingredient contained in the raw material for extraction is transferred by contacting the solvent with the raw material for extraction under specific conditions. You can include all regardless. For example, it may include all of the extraction of a component soluble in a solvent from a natural product using water or an organic solvent, and a product obtained by extracting only a specific component such as an oil, a specific component of a natural product, etc. In the present invention, it is particularly essential oil. However, it is not limited thereto.

In addition, all formulations that can be formed using the extract itself and the extract, such as extracts derived from the natural products listed above, diluents or concentrates of the extracts, dried products obtained by drying the extracts, preparations or purified products of the extracts, or mixtures thereof It may contain an extract of. Furthermore, the extract may be extracted from natural, hybrid or variant plants, and may be extracted from plant tissue culture.

The method for extracting the extract of the present invention can be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include cold needle extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, extrusion extraction, and the like, and these may be performed alone or in combination of two or more methods.

The type of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used as needed. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. In addition, the extract extracted as described above is filtered to remove suspended solid particles, for example, using nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., and then used as it is, or freeze-dried, hot-air drying, spray drying It can be used after drying using the like.

In the present invention, the "fermentation" refers to a process by which microorganisms decompose organic matter using enzymes. It is known that a raw material that has undergone a fermentation process by the microorganism has an effect of at least two to several tens of times higher than before fermentation. The fermented metabolites contain a large amount of amino acids, organic acids, and antioxidants that are beneficial to the skin, so it promotes skin metabolism and makes the skin texture elastic and smooth. In addition, during the fermentation process, the particles become smaller and toxicities such as heavy metals are decomposed, so that the absorption rate is increased, and skin problems or allergic side effects can be alleviated.

The "fermentation extract" may be prepared by diluting the extract in a solvent, specifically purified water to a certain concentration, inoculating a fermentation microorganism, and fermenting it.

The mixed fermented extracts of various natural substances listed above are the effects of the composition of the present invention due to the low molecular weight of physiologically active substances or the interaction of newly generated fermentation products during the fermentation process, improving blood circulation, decreasing scalp temperature, promoting dermal papilla cell growth, and hair thickness The effect of preventing hair loss such as strengthening may be increased, and not only the safety in the body but also the ability to penetrate the skin may be remarkably improved.

Specifically, in the fermentation step, 255 to 280 parts by weight of the mixed extract of the present invention and 200 to 250 parts by weight of purified water are uniformly mixed, and 30 to 40 parts by weight of the fermentation strain are inoculated thereto and aged at 35 to 40°C for 3 to 5 hours. It may be one, and more specifically, the fermentation strain is Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus helveticus ( Lactobacillus helveticus) , Lactobacillus rhamnosus ( Lactobacillus rhamnosus) , Bifidobacterium longum ( Bifidobacterium longum) ), Bifidobacterium lactis , Bifidobacterium animalis , Bifidobacterium bifidum , Lactobacillus casei and Enterococcus faecium ) may further include one or more selected probiotics from the group consisting of, and most specifically, the strain is mixed in a ratio of 1:2:1:2:1:3:1:0.5:1 by weight of each It may be manufactured by. However, it is not limited thereto, and may be appropriately changed and applied as necessary.

If the content of the extract is less than the stated range, the desired effect may not be sufficiently obtained, and if it exceeds the stated range, the irritation may be too strong and cause skin trouble.

In addition, the addition and mixing ratio of the probiotics has been established through the results of years of research, and if the above ratio is not observed, the growth of a specific strain is remarkable and the proliferation of other strains is suppressed, and the desired effect cannot be obtained.

Another aspect of the present invention provides a cosmetic composition comprising the composition.

Specifically, the cosmetic composition may have an effect of promoting skin blood circulation, reducing scalp temperature, promoting dermal papilla cell growth, and improving hair thickness, and each of these effects was confirmed through specific experimental examples.

In the present invention, the cosmetic composition includes shampoo, skin, toner, softening lotion, nutrient lotion, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, mist, It may be formulated as one or more selected from the group consisting of spray and powder. Specifically, the cosmetic composition may be formulated in the form of a shampoo, but is not limited thereto.

The cosmetic composition may be formulated by a conventional method. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the content disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions. , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial lotion and body lotion; Creams such as nourishing cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Alternatively, it may be formulated with a detergent such as a cleansing foam, soap, or body wash, but is not limited thereto. In addition, the cosmetic composition may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In the cosmetic composition, in addition to the essential ingredients in each formulation, other ingredients may be appropriately blended within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use.

The cosmetic composition may contain a generally acceptable carrier, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or their Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. Can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

By using only the extract derived from the natural product of the present invention, it is harmless and non-toxic to the human body, and even when applied to the human body, it does not cause side effects, so its utilization as a cosmetic composition is high. In addition, since the cosmetic composition of the present invention contains a large amount of active ingredients by the fermentation process, the effect of improving hair loss can be remarkably improved.

The cosmetic composition can be used for skin such as shampoo, skin, toner suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleaning oil, powder foundation, emulsion foundation, wax foundation, and spray. It is useful for use as an external preparation.

In addition, the present invention can create an optimal environment for promoting scalp growth by showing an excellent moisturizing effect when applied to the skin by including an extract derived from a natural product.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.

Preparation Example 1. Preparation of extract derived from natural products

In order to prepare an extract derived from a natural product of the present invention, a thief's cane root, red pepper fruit, goji berry, green tea, angelica root, beetroot root, mulberry fruit, mulberry root, giant pine leaf, chichi root, hawthorn root, lavender, bergamot leaves, Peppermint leaves, freesia, Matricaria flowers, and rosemary leaves were washed and dried to prepare an oil extract, each raw material was added to a commercial oil extractor tank, and the extractor was operated for 3 to 4 hours.

Specifically, the raw materials were placed in a distillation tank so that the ratio of purified water to each of the raw materials was about 1:2, and then distilled for 2 to 3 hours while maintaining the extraction temperature of the distillation tank at 90°C. At this time, the temperature of the cooling water supplied to the inside of the cooler was maintained at about 13°C, and the condensate passed through the cooler was collected in a separation tank.

The collected condensate is a mixture of oil and water, and when the oil and hydrosol are separated in the sorting tank, the hydrosol is received about 3 times, and the hydrosol residue containing the final oil is received and the hydrosol through the separator. And oil were separated. In order to obtain pure oil from the separated oil layer, the first separated oil was stored in a freezer for 1 day, and only unfrozen oil was collected to obtain essential oil as an extract.

The above extracts are used in 28 parts by weight of each thief's cane root extract, 17 parts by weight of pepper fruit extract, 19 parts by weight of goji berry extract, 15 parts by weight of green tea extract, 20 parts by weight of Korean Angelica root extract, 25 parts by weight of Goridae root extract, Mulberry fruit extract 22 parts by weight, 10 parts by weight of mulberry root extract, 15 parts by weight of giant pine leaf extract, 12 parts by weight of chichi root extract, 10 parts by weight of hawthorn root extract, 5 parts by weight of lavender extract, 5 parts by weight of bergamot leaf extract, 7 parts by weight of peppermint leaf extract Parts, 3 parts by weight of freesia extract, 3 parts by weight of Matricaria flower extract, and 3 parts by weight of rosemary leaf extract were mixed to prepare a composition for preventing hair loss and promoting hair growth of the present invention.

Example 2. Preparation of fermented extract derived from natural products

In order to enhance the efficacy of the extract derived from the natural product of the present invention, a fermentation strain was inoculated and fermented.

Specifically, 255 to 280 parts by weight of the mixed extract of Example 1 and 200 to 250 parts by weight of purified water were uniformly mixed, and 30 to 40 parts by weight of the fermentation strain were inoculated thereto and aged at 35 to 40°C for 3 to 5 hours.

In this case, the fermentation strain is Lactobacillus acidophilus , Lactobacillus helveticus , Lactobacillus rhamnosus , Bifidobacterium longum , Bifidobacterium longum Lactis ( Bifidobacterium lactis ), Bifidobacterium animalis ( Bifidobacterium animalis ), Bifidobacterium bifidum ( Bifidobacterium bifidum ), Lactobacillus casei ( Lactobacillus casei ) and Enterococcus faecium ( Enterococcus faecium ) are each 1: A mixture prepared by mixing at a weight ratio of 2:1:2:1:3:1:0.5:1 was used, but is not limited thereto.

Comparative Example 1. Preparation of a composition that does not contain gouridae, chichi and herbal extracts

In the manufacturing process of Example 2 above, the comparative example was carried out in the same manner except that the herbal extract consisting of copper stalk root, chichi root extract and lavender, bergamot leaf, peppermint leaf, freesia, matricaria flower and rosemary leaf was not included. The composition of 1 was prepared.

Experimental Example 1. Checking the effect of lowering the scalp temperature of the composition of the present invention

Twenty hair loss persons in their 30s to 60s were selected, and after waiting for 1 hour in constant temperature and humidity conditions (24 to 26℃, relative humidity 40 to 50%), heat was generated in the scalp through exercise for 15 minutes. . After washing the hair with a shampoo prepared including the compositions of Examples 1 and 2 and Comparative Example 1, skin temperature over time was measured immediately after shampooing, and the results are shown in Table 1 below.

At this time, the hair was wound with lukewarm water of 40 to 42°C, and a sufficient massage was performed for at least 5 minutes using a shampoo containing the composition of the present invention. As a shampoo used as a control, a commercially available shampoo for preventing hair loss was used.

time Temperature(℃) Example 1 Example 2 Comparative Example 1 Control After exercise (-10 minutes) 33.2 33.3 33.4 33.2 Immediately after shampooing (0 minutes) 37.3 37.2 37.4 37.1 10 minutes 34.9 32.9 36.0 35.8 20 minutes 32.3 31.5 33.6 33.5 30 minutes 30.9 30.4 31.1 32.1

As shown in Table 1, both Examples 1 and 2 showed excellent scalp temperature drop effect in skin temperature change, and it was confirmed that a relatively lower temperature was maintained over time, and in particular, Example 2 was the most effective. In particular, in the case of Comparative Example 1 and the control group, it was confirmed that the scalp temperature was slightly decreased but not significantly decreased, and still showed a higher temperature compared to Example 2 even after the passage of time.

From the above results, it was confirmed that in Examples 1 and 2, the scalp temperature was rapidly decreased and at the same time, the scalp temperature was continuously maintained at a low temperature, thereby substantially preventing hair loss.

Experimental Example 2. Confirmation of dermal papilla cell growth promoting effect through MTT-assay

Papilla cells exist in the dermal cell layer and play an important role in hair growth, so it was confirmed whether the composition of the present invention has an effect of promoting the growth of dermal papilla cells.

For cell culture, a modified medium containing fetal bovine serum (FBS) and penicillin-streptomycin was used, based on DMEM (Dulbecco's Modified Eagle Medium) medium, and the cells were 37°C, 5% CO ₂ conditions. Cultured in. Cultured dermal papilla cells were exchanged twice a week, washed with PBS every 6 to 7 days, separated cells attached with 0.05% trypsin-0.02% EDTA, centrifuged, and used in the experiment while subculturing I did.

The dermal papilla cells were dispensed into a 96 well plate at a rate of 1×10 cells/well 200 μL each, and cultured at 37° C. and 5% CO ₂ for 18 hours to allow the cells to adhere. After 18 hours, the medium was removed, and 200 μL of a sample was added to the well to which the cells were attached and cultured for 24 hours. After removing the medium from the cultured cells, 100 μL of 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazoliumbromide (MTT) solution prepared at a concentration of 500 ug/mL was added to PBS for 4 hours under the same culture conditions. It was further cultured. At this time, the generated formazan crystal was dissolved in DMSO and the absorbance was measured at 540 nm with an ELISA reader, and the measured values within the OD range of 0.00∼3.50 were used for the experimental results.

The experiment was carried out at a concentration of 0.5% each of the compositions of Examples 1 and 2 and Comparative Example 1 of the present invention, and the cell viability is shown in Table 2 below.

Example 1 Example 2 Comparative Example 1 Control (distilled water) Cell viability (based on 0.5%) ++ +++ + -

As shown in the results shown in Table 2, when the compositions of Examples 1 and 2 of the present invention were treated, it was confirmed that cell viability increased by promoting the growth of dermal papilla cells, and in particular, the results of Example 2, which is a fermented extract, were more excellent. On the other hand, it was confirmed that the cell viability of Comparative Example 1, which is a composition excluding gouridae, chichi, and herbal extracts, significantly decreased, and that the control group did not show any effect.

Experimental Example 3. Confirmation of hair thickness improvement effect

100 ml of the compositions of Examples 1 and 2 and Comparative Example 1 were put into a spray container and sprayed twice a day (morning and evening) onto the scalp of the hair loss area of a patient with alopecia, and the effect was confirmed. The compositions of Examples and Comparative Examples were used twice a day for about 60 days, and after they were used, they were prepared and used in the same manner as above. In the control group, minoxidil external preparation was applied twice a day to the hair loss area and the scalp of the expected hair loss area, and the effect was confirmed.

The alopecia patients are 20 alopecia patients in their 30s to 60s who are the same as those selected in Experimental Example 1 below, diagnose the hair condition at the same time as the start of the experiment, tattoo a black dot on the area with the most severe hair loss, and measure the same for each measurement. Measures were taken so that the site could be measured. Then, the number of hairs per unit area (number of hairs/cm2) and hair thickness (µm) were measured using a hair meter (Folliscope, Reed M).

For the number of hairs per unit area, the number of terminal hairs in the unit area was calculated after photographing the hair loss area around the display area using a 30x lens. The thickness of the hair was averaged after measuring the thickness of 5 strands around the display area using a 200x lens.

Changes in the number of hairs (number of hairs/cm2) measured at the time point before spraying the composition of the present invention and after 15, 30, 45, and 60 days elapsed and changes in hair thickness (㎛) are shown in Tables 3 and 4 below. Shown in.

time Increased number of hairs (number of hairs/cm ² ) Example 1 Example 2 Comparative Example 1 Control First measurement (+0 days) - - - - After +15 days 12 15 7 0 After +30 days 19 26 9 -One After +45 days 28 30 10 3 After +60 days 32 35 6 One

time Increased hair thickness (△㎛) Example 1 Example 2 Comparative Example 1 Control First measurement (+0 days) - - - - After +15 days 0.007 0.006 0.002 -0.012 After +30 days 0.009 0.013 0.005 0.010 After +45 days 0.015 0.019 0.010 0.001 After +60 days 0.019 0.023 0.009 -0.005

As shown in Tables 3 and 4, it was confirmed that the compositions of Examples 1 and 2 of the present invention showed a positive effect on both the number and thickness of hair, and Comparative Example 1 showed a somewhat inferior effect, and the control group was rather tested. In particular, it was confirmed that the effect of the composition of Example 2 of the present invention was increased through fermentation.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains will be able to understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be construed as being included in the scope of the present invention.

Claims (5)

In the composition for preventing hair loss and promoting hair growth, comprising a mixed extract derived from a natural product fermented by inoculating a fermented strain,
The natural product-derived mixed extract is 28 parts by weight of a thief's cane root extract, 17 parts by weight of red pepper fruit extract, 19 parts by weight of goji berry extract, 15 parts by weight of green tea extract, 20 parts by weight of Angelica root extract, 25 parts by weight of beetroot extract, mulberry Fruit extract 22 parts by weight, Mulberry root extract 10 parts by weight, Giant pine leaf extract 15 parts by weight, Chichi root extract 12 parts by weight, Sewage root extract 10 parts by weight, Lavender extract 5 parts by weight, Bergamot leaf extract 5 parts by weight, Peppermint leaf extract 7 parts by weight, 3 parts by weight of freesia extract, 3 parts by weight of Matricaria flower extract, and 3 parts by weight of rosemary leaf extract,
The fermentation is a fermentation strain Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus helveticus ( Lactobacillus helveticus) , Lactobacillus rhamnosus ( Lactobacillus rhamnosus) , Bifidobacterium longum ( Bifidobacterium longum ), Bifidobacterium longum Lactis ( Bifidobacterium lactis ), Bifidobacterium animalis ( Bifidobacterium animalis ), Bifidobacterium bifidum ( Bifidobacterium bifidum ), Lactobacillus casei ( Lactobacillus casei ) and Enterococcus faecium ( Enterococcus faecium ) is used. , Hair loss prevention and hair growth promotion composition. The method of claim 1,
The extract is water; Methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, butylene glycol, propylene glycol, methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, dichloromethane, or a mixture thereof extracted using the extraction solvent Phosphorus, a composition for preventing hair loss and promoting hair growth. A composition for external application for skin for preventing hair loss and promoting hair growth, comprising the composition of claim 1 or 2. A cosmetic composition for preventing hair loss and promoting hair growth, comprising the composition of claim 1 or 2. The method of claim 4,
The cosmetic composition includes shampoo, skin, toner, softening lotion, nutrient lotion, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, mist, spray and powder Formulated with one or more selected from the group consisting of, a cosmetic composition.