KR102434209B1 - Hair composition for preventing hair loss and promoting hair growth comprising natural oils - Google Patents

Abstract

The present invention relates to a composition comprising natural oil for cosmetic or skin external application for promoting hair growth and preventing hair loss and, more specifically, to a composition composed of orange peel oil, rosemary flower oil, cedarwood leaf oil, lavender flower oil, roman chamomile flower oil, ylang flower oil, and melaleuca leaf oil for cosmetic or skin external application for promoting hair growth and preventing hair loss. The composition according to the present invention can obtain the effect of promoting hair growth and preventing hair loss from the safe and excellent dermal papilla cell proliferation effect and excellent 5-alpha reductase activity inhibitory effect without problems such as side effects caused by synthetic compounds contained in general cosmetics and skin external preparations.

Description

Composition for promoting hair growth and preventing hair loss containing natural oil

The present invention relates to a composition for promoting hair growth and preventing hair loss, comprising orange peel oil, rosemary oil, cedarwood oil, lavender oil, roman chamomile oil, ylang ylang oil, and melaleuca oil.

Human hair differs in color and shape according to race, and it is common to dye it in various colors or perm in various forms for the purpose of beauty. In addition, according to the modern cultural trend that emphasizes individuality, everyone dyes their hair or perms to reveal their individuality in various ways.

However, chemicals such as dyes and perms used for dyeing or perm are made of chemical components and are highly toxic, adversely affecting hair and scalp. These toxins penetrate into the human body through the skin, which is called transdermal poisoning. Most of the transdermal poisons are derived from soap, shampoo, conditioner, body lotion, bath detergent, cosmetics, laundry detergent, hair dye, and perm. In addition, transdermal poison causes hair loss when it penetrates the scalp, and there is a fatal problem of inhibiting hair growth.

In particular, hair loss refers to a state in which there is no hair in the area where hair should normally exist, and generally refers to the loss of hair on the scalp. All hairs stop growing after a certain period of growth, pass the catagen phase, go into the resting phase, and repeat the process of hair loss and hair growth again. This is called the hair growth cycle. In particular, the hair growth period is longer than 2 to 5 years and the rest period is short, less than 2 to 3 months. The hair that enters the resting period is light in color and lacks luster, and the root of the hair is thin. Excessive hair loss due to abnormalities in the hair growth cycle is called hair loss.

Most of the previously developed hair growth agents or hair growth agents are those that prevent hair loss and induce hair growth by improving indirect causes of baldness such as poor blood circulation, nutritional disorders, seborrheic dermatitis, and stress, and their effects were not perfect. In addition, if you stop using it, the hair starts to fall out again and returns to the original hair loss state, so there is a disadvantage that the effect is not continuous. Minoxidil (MXD) and finasteride, which are representative hair growth agents with well-known medicinal effects and widely used worldwide, inhibit the production of DHT that causes hair loss by inhibiting 5α-Reductase, which converts testosterone into DHT. (Yamana K et al., Horm Mol Biol Clin Investig., 1,2(3):293-299, 2010; Clark RV et al., J Clin Endocrinol Metab., 89(5):2179 -2184, 2004). However, in order to maintain the effect of hair growth and hair loss prevention, these hair loss treatments require continuous use throughout life, and their use is limited due to side effects and difficulties in continuation.

Accordingly, the present inventors have been conducting research to find raw materials having excellent stability among substances obtained from natural materials and particularly having effects on improving hair loss and hair growth, orange peel oil, rosemary oil, cedarwood oil, lavender oil, roman chamomile oil, ylang It was found that a composition in which ylang oil and melaleuca oil were mixed in an optimal ratio was very effective in preventing hair loss and promoting hair growth, and the present invention was completed.

Korean Patent No. 10-1709918

Yamana K et al., Horm Mol Biol Clin Investig., 1,2(3):293-299, 2010; Clark RV et al., J Clin Endocrinol Metab., 89(5):2179-2184, 2004

In order to solve the above problems, the present invention is to provide a cosmetic or external composition for skin application for preventing hair loss and preventing hair loss due to the proliferation of dermal papilla cells and inhibition of 5-alpha reductase activity and excellent hair growth promoting effect. .

Another object of the present invention is to provide a method for promoting hair growth of an individual and preventing hair loss by administering the composition to an individual.

In one aspect, the present invention provides a cosmetic composition for promoting hair growth and preventing hair loss comprising orange peel oil, rosemary oil, cedarwood oil, lavender oil, roman chamomile oil, ylang ylang oil, and melaleuca oil.

In the present invention, "hair loss" refers to a state in which there is no hair in a region where hair should normally exist, and specifically refers to the loss of hair (thick and dark hair) of the scalp. The cause of hair loss is not limited, but there are, for example, hereditary androgenic alopecia (baldness), alopecia areata, ringworm of the head due to fungal infection, telogen hair loss, hair growth wall, hair growth disorder disease, etc. may mean hair loss due to lupus, folliculitis solitary, lichen planus pilaris, hair loss due to burns and trauma.

The term "prevention" of the present invention means preventing any thing or phenomenon from occurring, and in the present invention, hair loss prevention means preventing hair loss from occurring.

In the present invention, "hair growth" means that hair grows in the hair follicle. The hair includes hair roots and follicles of the head, hair and eyelashes, eyebrows, beards, armpits, pubic hair, and all parts of the body having hair roots and follicles.

In the present invention, the hair loss prevention or hair growth promoting effect may be achieved by promoting the proliferation of dermal papilla cells by the composition consisting of the oil and inhibiting the activity of 5-alpha reductase.

The cosmetic composition according to the present invention comprises 8 to 22: 8 to 12: 8 to 22: 8 to 12: orange peel oil, rosemary oil, cedarwood oil, lavender oil, roman chamomile oil, ylang ylang oil, and melaleuca oil, respectively. 8 to 12: 8 to 12: 18 to 32 by weight, preferably 10 to 22: 8 to 12: 10 to 22: 8 to 12: 8 to 12: 8 to 12: 20 to 32 by weight, more preferably is 13 to 17: 8 to 12: 13 to 17: 8 to 12: 8 to 12: 8 to 12: a weight ratio of 25 to 32, more preferably 14 to 16: 9 to 11: 14 to 16: 9 to 11: 9 to 11: 9 to 11: 28 to 32, and most preferably 15: 10: 15: 10: 10: 10: 30 by weight.

When each oil is contained in the above-mentioned weight ratio, the effect of inhibiting dermal papilla cell proliferation and 5-alpha reductase activity is excellent, so that the purpose of promoting hair growth and preventing hair loss according to the present invention can be achieved.

In addition, the components constituting the cosmetic composition according to the present invention are preferably extracted by an oil extraction method suitable for each component.

Specifically, the orange peel oil and cedarwood oil are each of orange peel and cedarwood leaves that are not dried in a solution containing calcium salt at room temperature for 50 minutes to 70 minutes, preferably 55 minutes to 65 minutes, most preferably After immersion for about 60 minutes, it is preferably prepared by centrifugation after compression at a low temperature, preferably 15 to 25 ℃, more preferably 18 to 22 ℃, even more preferably 19 to 21 ℃. The proliferation of dermal papilla cells was effective when the immersion of the calcium salt before compression, the immersion time and the compression temperature time were met, and thus, it is possible to achieve the effect of promoting hair growth and preventing hair loss.

In addition, when orange peel oil and cedarwood oil were prepared by the above method, the proliferation effect of dermal papilla cells was remarkably excellent compared to oils prepared by other extraction methods, and increased by being contained in the cosmetic composition according to the present invention. It has the effect of promoting hair growth and preventing hair loss.

The rosemary oil, lavender oil, roman chamomile oil, and ylang-ylang oil were obtained by solvent extraction. More specifically, after adding an extraction solvent 1 to 15 times by weight to each of rosemary flowers, lavender flowers, Roman chamomile flowers, and ylang-ylang flowers, 30-50° C., preferably 35-48° C., more preferably 42 An oil containing an active ingredient may be obtained by dissolving the active ingredient of the natural raw material at a temperature of ~46°C, most preferably 45°C for 1 to 5 hours and separating the extraction solvent again. At this time, if the extraction temperature is less than 30 ℃, the oil may not be properly extracted from the raw material, and on the contrary, if it exceeds 50 ℃, the active ingredient contained in the oil is destroyed or deformed by heat, so that the effect of promoting hair growth and preventing hair loss cannot be properly expressed. .

The solvent extraction method does not apply heat or pressure to the raw material, so that the highly concentrated oil can be extracted without damaging the raw material itself, and can be extracted with a high yield. In addition, the unique flavor of the raw material can be maintained without destroying it. At this time, the extraction solvent may be obtained by filtration after extraction with one or more extraction solvents selected from the group consisting of hexane, ether, ethyl alcohol and isopropyl alcohol alone or in combination. Preferably, the extraction solvent may be ether.

Rosemary oil, lavender oil, Roman chamomile oil, and ylang-ylang oil prepared by the above method have significantly superior proliferation effect on dermal papilla cells compared to oils prepared by other extraction methods, and are contained in the cosmetic composition according to the present invention By doing so, it exhibits increased hair growth promotion and hair loss prevention effect.

The melaleuca oil was obtained by subcritical extraction. More specifically, after drying and pulverizing the leaves of Melaleuca, it is placed in a subcritical extractor at 75 to 85 ° C, preferably 78 to 82 ° C, most preferably 89 ° C to 55 to 65 atmospheres (atm), preferably 58 to 62 At a flow rate of 5 mL/min at atmospheric pressure (atm), most preferably 60 atmospheres (atm), for 70 to 90 minutes, preferably for 75 to 85 minutes, more preferably for 78 to 82 minutes, most preferably for 80 minutes It can be prepared by extraction. Melaleuca oil extracted under these conditions has a significantly superior effect on the proliferation of dermal papilla cells compared to Melaleuca oil prepared by other extraction methods, and by being contained in the cosmetic composition according to the present invention, it exhibits increased hair growth promotion and hair loss prevention effects.

The above-mentioned seven types of oils were selected as oils having excellent proliferation effects among them by comparing and analyzing the effects of dermal papilla cell proliferation from dozens of oils. In addition, the extraction method for the selected 7 types of oil was selected from the effect of inhibiting dermal papilla cell proliferation and 5-alpha reductase activity. In addition, when these selected 7 types of oils are mixed in an optimal ratio, the effect of promoting hair growth and preventing hair loss is excellent.

On the other hand, since the cosmetic composition of the present invention is composed of only 7 types of oils, it can be used in formulations such as hair tonic, hair nourishing lotion, hair essence, hair wax, and the like.

As another aspect, the present invention provides a composition for external application for skin for promoting hair growth and preventing hair loss, comprising orange peel oil, rosemary oil, cedarwood oil, lavender oil, roman chamomile oil, ylang ylang oil, and melaleuca oil.

The seven types of oils and their effects are as described above.

Since the composition for external application for skin of the present invention is composed of only 7 types of oils, it can be used in formulations such as solutions, gels, emulsions, sprays, etc.

On the other hand, the cosmetic and skin external composition of the present invention consists only of 7 types of oils derived from nature and does not contain any other ingredients, so there is no problem such as side effects on human skin caused by synthetic compounds contained in cosmetics. It is possible to achieve the effect of promoting hair growth and preventing hair loss due to the excellent effect of inhibiting the proliferation of dermal papilla cells and the activity of 5-alpha reductase.

The cosmetic composition for promoting hair growth and preventing hair loss according to the present invention and the composition for external application for skin contain as essential ingredients 7 kinds of natural oils obtained by an extraction method that can achieve remarkably excellent properties of raw materials and effects of promoting hair growth and preventing hair loss. By mixing these in the optimal ratio, it is possible to obtain the effect of promoting hair growth and preventing hair loss by the effect of inhibiting the proliferation of dermal papilla cells and the activity of 5-alpha reductase while safely using it without problems such as side effects caused by synthetic compounds contained in general cosmetics.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Preparation Example 1: Oil Preparation

1-1. Oil production by pressing method

Orange peel, grapefruit peel, lemon peel, mandarin peel, and bergamot peel, cedarwood leaf, sandalwood root or stem, rosemary leaf or flower, lavender leaf or flower, roman chamomile leaf or flower, ylang ylang flower, melaleuca leaf or Three times the weight of ethyl alcohol was added to each of flowers, eucalyptus leaves, jasmine leaves or flowers, juniper berry leaves or fruits, and cardamom seeds and pressed, followed by distillation at a temperature of 0° C. to obtain each oil.

1-2. Oil production by solvent extraction

Each of the preparations in 1-1 was dried and powdered, dissolved by adding ether, and distilled at a temperature of 45° C. to obtain each essential oil.

Example 1: Oil selection through hair cell proliferation effect analysis

In order to find out what effect the oil prepared in Preparation Example 1 has on the proliferation of dermal papilla cells, which are the main cells of hair growth, the dermal papilla cell proliferation ability was measured. Specifically, human dermal papilla cells, HFDPC (Hair follicle dermal papilla cell, PromoCell), were cultured in a dermal papilla cell growth medium containing supplementMix and then inoculated into a 24-well plate (1.5×10 ⁴ cells/well), 5% concentration. of CO ₂ in an incubator at 37° C., and incubated for 24 hours. Then, the prepared oil was dissolved in DMEM medium containing 1% FBS, treated in each cultured well, and incubated again for 72 hours. After 72 hours, the cell culture supernatant was removed, washed once with PBS, diluted 10-fold with MTT reagent dissolved in 1 mg/ml, mixed with the medium, and then treated and cultured for 1 hour. After 1 hour, after removing the MTT reagent, 200 μl of DMSO was added per well, formazan was dissolved, and the absorbance value measured at 540 nm was compared with the absorbance value of the dermal papilla cell growth medium group as a control group and compressed in Table 1 below. Results according to the method, Table 2 below shows the results according to the solvent extraction method.

Oil prepared by pressing (1 μl) Cell viability (%) Oil prepared by solvent extraction (1 μl) cell
viability (%) orange peel oil 112 orange peel oil 104 grapefruit peel oil 95 grapefruit peel oil 90 lemon peel oil 96 lemon peel oil 88 mandarin peel oil 99 mandarin peel oil 85 Bergamot Peel Oil 98 Bergamot Peel Oil 87 Cedarwood Leaf Oil 108 Cedarwood Leaf Oil 98 sandalwood root oil 102 sandalwood root oil 92 sandalwood stem oil 98 sandalwood stem oil 94 rosemary leaf oil 102 rosemary leaf oil 102 rosemary flower oil 103 rosemary flower oil 108 lavender leaf oil 100 lavender leaf oil 98 lavender flower oil 100 lavender flower oil 110 Roman Chamomile Leaf Oil 86 Roman Chamomile Leaf Oil 94 Roman Chamomile Flower Oil 92 Roman Chamomile Flower Oil 114 Ylang Ylang Flower Oil 98 Ylang Ylang Flower Oil 108 Melaleuca Leaf Oil 104 Melaleuca Leaf Oil 106 Melaleuca Flower Oil 100 Melaleuca Flower Oil 104 eucalyptus leaf oil 92 eucalyptus leaf oil 98 jasmine leaf oil 96 jasmine leaf oil 98 jasmine flower oil 93 jasmine flower oil 102 juniper berry leaf oil 92 juniper berry leaf oil 94 juniper berry fruit oil 96 juniper berry fruit oil 94 cardamom seed oil 95 cardamom seed oil 88

As shown in Table 1, orange peel oil and cedarwood leaf oil prepared by the pressing method, rosemary flower oil prepared by solvent extraction method, lavender flower oil, roman chamomile flower oil, ylang ylang flower oil, and melaleuca leaf oil were These oils were selected because of their superior cell proliferation effect on dermal papilla cells compared to other oils.

Example 2: Comparison of the proliferation effect of dermal papilla cells of oil according to the extraction method

The proliferation effect of dermal papilla cells of the oil according to the extraction method was compared for the oils selected in Example 1 above.

A specific extraction method is an oil obtained by immersing 200 g of dried skins, flowers, or leaves in a 10% aqueous calcium chloride solution for 60 minutes in addition to the extraction method performed in Example 1, then pressing and centrifuging at 60° C. (Preparation Example 2) , 200 g of oil (Preparation Example 3), peel, flower, or leaf prepared in the same manner as in Preparation Example 2, but at a temperature of 20° C. during compression, in a subcritical extractor at 80° C. and 60 atm (atm) for 80 minutes Oil (Preparation Example 4) prepared by extracting during the period was performed in the same manner as in Example 1 of the dermal papilla cell proliferation experiment. The results are shown in Table 2 below.

oil Cell viability (%) Preparation 2 Preparation 3 Preparation 4 orange peel oil 114 120 104 Cedarwood Leaf Oil 107 118 101 rosemary flower oil 106 105 105 lavender flower oil 100 102 105 Roman Chamomile Flower Oil 92 101 112 Ylang Ylang Flower Oil 98 104 106 Melaleuca Leaf Oil 104 105 110

As can be seen in Table 2, orange peel oil and cedarwood leaf oil had the best effect on dermal papilla cell proliferation of the oil prepared according to the extraction method of Preparation Example 3. Rosemary flower oil, lavender flower oil, Roman chamomile flower oil, and ylang-ylang flower oil had the best effect on dermal papilla cell proliferation of the oil prepared according to the solvent extraction method of Preparation Example 1-2 and the subcritical extraction method of Preparation Example 4 , considering the time and cost of the extraction process, the solvent extraction method of Preparation Example 1-2 was most suitable. In contrast, the melaleuca leaf oil had the best effect on dermal papilla cell proliferation of the oil prepared according to the subcritical extraction method of Preparation Example 4.

Example 3: Comparison of the proliferation effect of dermal papilla cells of mixed oil according to the composition of the oil

The oil according to the extraction method selected according to Example 2 was mixed in the weight ratio described in Table 3 below, and the proliferation effect of dermal papilla cells was compared in the same manner as in Example 1. In particular, when the results of Examples 1 and 2 show the best effect on the proliferation of dermal papilla cells, the optimal mixing ratio was derived based on the case where the contents of orange peel oil and cedarwood leaf oil were increased compared to the contents of other oils. . The results are shown in Table 4 below.

oil Content (wt%) production example
5 production example
6 production example
7 production example
8 production example
9 production example
10 orange peel oil 20 30 30 20 15 15 Cedarwood Leaf Oil 20 30 20 30 15 15 rosemary flower oil 10 5 5 5 10 10 lavender flower oil 10 10 10 10 10 10 Roman Chamomile Flower Oil 10 10 10 10 10 20 Ylang Ylang Flower Oil 10 5 5 5 10 10 Melaleuca Leaf Oil 20 10 20 20 30 20

production example
5 production example
6 production example
7 production example
8 production example
9 production example
10 Cell viability (%) 130 120 126 128 138 125

As can be seen in Table 4, the dermal papilla cell proliferation effect of the oil composition of Preparation Example 9 was the most excellent, followed by the dermal papilla cell proliferation effect in the order of Preparation Example 5 and Preparation Example 7. In particular, it was found that the proliferative effect of dermal papilla cells was further increased when the content of melaleuca leaf oil was higher than that of other oils compared to the case of the single oil composition.

Example 4: Measurement of the inhibitory effect of 5-alpha reductase activity of mixed oil according to oil composition

The oil composition mixed in Example 4 was added to the testosterone reaction solution, and the ability to inhibit 5-alpha reductase activity was measured. Specifically, after mixing 1 mmol/L DTT (dithiothreitol), pH 6.5, 40 mmol/L potassium phosphate, and 100 μmol/L NADPH, 3.5 μmol/L testosterone was added, and finally 0.5 ml of A testosterone reaction solution was prepared. After leaving at room temperature for 20 minutes, 25 ppm of finasteride was treated as a positive control, 10 ppm or 50 ppm of the oil composition of Example 4 was treated in another reaction solution, and rat liver granule was added to the total reaction solution for 30 minutes. The reaction was carried out at 37°C. After 30 minutes of lyophilization, the amount of testosterone remaining was analyzed by HPLC.

Testosterone was analyzed using a Waters 2695 Separation Module, Waters 2996 Photodiode Array Detector through a Phenomenex Luna C18 4.6 × 150 mm, 2.6 μm (reversed phase) column. For the analysis, a sample dissolved in 0.5 ml of methanol was used as a sample solution, and 2.0 mg of a 98% pure Testosterone standard was precisely weighed and 40 ml of a solution dissolved in methanol was used as a testosterone standard stock solution (50 ppm). Precisely weigh 1 ml of this solution, add methanol to dissolve it, and make 10 ml as the standard solution (5 ppm). The test solution and the standard solution were analyzed according to the indicator component analysis method to determine the testosterone content in each sample using Equation 1 below. was calculated through

[Equation 1]

STD concentration (㎍/㎖) × A Test /A STD × dilution factor × (100(%) / 1(㎍/㎖)) × (STD purity)

The results obtained from this were converted based on 100% of the control group, and the results are shown in Table 5 below.

Residual testosterone content (%) Oil composition 10ppm Oil composition 50ppm Preparation 5 78 92 Preparation 6 65 77 Preparation 7 68 82 Preparation 8 72 84 Preparation 9 81 98 Preparation 10 68 84 Finasteride 25ppm 85

As can be seen from the above results, the oil composition of Preparation Example 9 exhibited almost similar or superior 5-alpha reductase inhibitory ability to finasteride as a positive control. Next, the oil composition of Preparation Example 5 was excellent in inhibiting 5-alpha reductase.

From the above results, it can be seen that the oil composition according to the present invention is effective in promoting hair growth and preventing hair loss due to its excellent ability to inhibit dermal papilla cells and 5-alpha reductase.

Claims (6)

A cosmetic composition for promoting hair growth and preventing hair loss, comprising orange peel oil, rosemary flower oil, cedarwood leaf oil, lavender flower oil, roman chamomile flower oil, ylang ylang flower oil, and melaleuca leaf oil. According to claim 1,
The orange peel oil and cedarwood leaf oil are obtained by immersing each non-dried orange peel and cedarwood leaf in a solution containing calcium salt for 50 to 70 minutes at room temperature, followed by compression and centrifugation at 15 to 25° C. used oil,
The rosemary flower oil, lavender flower oil, Roman chamomile flower oil, and ylang-ylang flower oil are oils obtained by solvent extraction,
The melaleuca leaf oil is a cosmetic composition for promoting hair growth and preventing hair loss, characterized in that it is an oil obtained by extraction for 70 to 90 minutes at 75 to 85° C. and 55 to 65 atmospheres (atm) in a subcritical extractor. The method of claim 1,
The orange peel oil, rosemary flower oil, cedarwood leaf oil, lavender flower oil, roman chamomile flower oil, ylang ylang flower oil, and melaleuca leaf oil are 8 to 22: 8 to 12: 8 to 22: 8 to 12: 8 to 12: 8 to 12: A cosmetic composition for promoting hair growth and preventing hair loss, characterized in that it consists of a weight ratio of 18 to 32. An external skin composition for promoting hair growth and preventing hair loss, comprising orange peel oil, rosemary flower oil, cedarwood leaf oil, lavender flower oil, roman chamomile flower oil, ylang ylang flower oil, and melaleuca leaf oil. 5. The method of claim 4,
The orange peel oil and cedarwood leaf oil are obtained by immersing each non-dried orange peel and cedarwood leaf in a solution containing calcium salt for 50 to 70 minutes at room temperature, followed by compression and centrifugation at 15 to 25° C. used oil,
The rosemary flower oil, lavender flower oil, Roman chamomile flower oil, and ylang-ylang flower oil are oils obtained by solvent extraction,
The melaleuca leaf oil is a skin external composition for promoting hair growth and preventing hair loss, characterized in that it is an oil obtained by extraction for 70 to 90 minutes at 75 to 85° C. and 55 to 65 atmospheres (atm) in a subcritical extractor. 5. The method of claim 4,
The orange peel oil, rosemary flower oil, cedarwood leaf oil, lavender flower oil, roman chamomile flower oil, ylang ylang flower oil, and melaleuca leaf oil are 8 to 22: 8 to 12: 8 to 22: 8 to 12: 8 to 12: 8 to 12: A composition for external application for skin for promoting hair growth and preventing hair loss, characterized in that it consists of a weight ratio of 18 to 32.