KR102567315B1 - Cosmetic composition for gray hair containing fermented extracts of natural ingredients - Google Patents

Abstract

The present invention relates to a composition for gray hair containing a mixed fermented extract of natural products, and specifically, contains a mixed fermented extract of monoginasansa leaf, kava kava root, and red bean as active ingredients to suppress hydrogen peroxide, which causes gray hair, It relates to a cosmetic composition for hair that exhibits excellent gray hair improvement activity by increasing melanin production and has excellent hair and scalp protection effects.

Description

Composition for gray hair containing fermented extracts of natural substances {Cosmetic composition for gray hair containing fermented extracts of natural ingredients}

The present invention relates to a composition for gray hair containing a mixed fermented extract of natural products, and specifically, contains a mixed fermented extract of monoginasansa leaf, kava kava root, and red bean as active ingredients to suppress hydrogen peroxide, which causes gray hair, and , It relates to a cosmetic composition for hair that exhibits excellent gray hair improvement activity by increasing melanin production and has excellent hair and scalp protection effects.

In human hair, the content of melanin pigment is reduced due to aging and stress, and gray hair and gray hair grow. Due to the extension of life expectancy and external environmental factors or stress, the gray-haired population is gradually increasing, and the population who dyes their pale hair darker for a younger appearance is also steadily increasing.

Recently, products such as hair colorants and hair manicures are widely used to solve gray hair, but these products have a very temporary color development effect, and permanent dyes and permanent hair dyes cannot color the roots of newly growing hair. There is a problem of poor durability. Therefore, the development of a product that suppresses the occurrence of gray hair or returns it to its original color is still insufficient.

The main reason for gray hair is that hydrogen peroxide bleaches the hair. When hydrogen peroxide builds up in the hair follicles, it inhibits the synthesis of melanin, the pigment that makes hair dark, and this causes the hair to turn white.

The inventors of the present invention focused on the fact that the suppression of melanin synthesis by hydrogen peroxide is an important cause of gray hair, and researched to develop a natural gray hair improvement cosmetic using a natural product that inhibits the activity of hydrogen peroxide and a natural product that increases melanin production. The present invention was completed by confirming that the mixed fermented extract of natural products prepared by fermenting and extracting ginasansa leaves, kava kava roots, and red beans by lactic acid bacteria has excellent hydrogen peroxide activity inhibition and exhibits an excellent melanin production ability to improve gray hair.

(0001) Patent Publication No. 10-2019-0143627 (2019.12.31) (0002) Patent Publication No. 10-2016-0037016 (2016.04.05)

An object of the present invention is to provide a composition for hair containing a mixed fermented extract of monoginasansa leaf, kava kava root and adzuki bean, and exhibiting excellent gray hair improvement effect and hair and scalp protection effect.

In order to achieve the above object, according to the present invention, a cosmetic composition for hair containing as active ingredients a mixed fermented extract of monoginasansa leaf, kava kava root, and red bean is provided.

The mixed fermented extract is characterized in that it is prepared by fermenting the mixed extract of monoginasansansa leaf, kava kava root and adzuki bean with lactic acid bacteria.

The lactic acid bacteria are Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae , Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei and Lactobacillus crispatus. It is at least one selected from the group consisting of, more preferably Lactobacillus plantarum.

The mixed extract is characterized in that the extracts are mixed and extracted in a weight ratio of 0.1 to 0.5: 0.5 to 1: 0.5 to 1, respectively.

The mixed fermented extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition for hair is characterized in that it is for improving gray hair.

The cosmetic composition for hair is characterized in that for hair and scalp protection.

The mixed fermented extract of monoginasansa leaf, kava kava root, and adzuki beans of the present invention is safe as a natural extract, and its synergistic effect suppresses hydrogen peroxide and increases melanin production. Therefore, it can be usefully used as a cosmetic composition for hair.

Hereinafter, the present invention will be described in detail.

The present invention is a natural product mixed fermented extract obtained by mixing and extracting monoginasansa leaves, which suppresses the activity of hydrogen peroxide, and kava cava roots and red beans, which have an activity to increase the melanin content, which is a natural pigment, and then fermented with lactic acid bacteria. It is characterized by the fact that it shows the effect of improving gray hair and protecting the hair and scalp.

Crataegus monogyna belongs to the genus Hawthorn and is also called Western Sansa. It is characterized by more thorns than the wild rose in Korea, and has been used as a diuretic in Europe for a long time. It is also said to relieve pain, such as muscle pain or arthritis, and stiff skin. In the present invention, an extract is prepared using the leaves of Monogina hawthorn.

Kava kava (Piper methysticum) is an herb that has been used medicinally for centuries. It contains ingredients such as kavalactone, flavocabine, and chalcone, and the root contains a compound known as kavalactone, which is known to help soothe and stabilize the skin and impart comfort to the skin.

Red bean (Vigna angularis) is a climbing annual plant or its seed of the Leguminosae genus Vigna. Red beans contain vitamin B1, vitamins B2, A, and minerals, and the saponin component of red beans lowers cholesterol levels in blood vessels and facilitates blood flow. Because of this, nutrients are well supplied to the capillaries, which promotes blood circulation and makes the scalp healthy. In addition, the anthocyanin component of red beans has an excellent antioxidant effect and removes active oxygen that causes aging, which is effective for skin beauty.

According to the present invention, there is provided a cosmetic composition for hair containing monoginasansa leaf, kava kava root, and mixed fermented extract of red beans as active ingredients.

The mixed fermented extract is prepared by fermenting a mixed extract of monoginasansansa leaf, kava kava root, and red bean with lactic acid bacteria.

At this time, the lactic acid bacteria are Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus cerevisiae (Pediococcus) cerevisiae), Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei and Lactobacillus crispatus ) At least one selected from the group consisting of, more preferably Lactobacillus plantarum.

According to one embodiment of the present invention, the mixed fermented extract is prepared by extracting a mixture of monogina hawthorn leaves, kava kava roots and red beans to prepare a mixed extract, and then using lactic acid bacteria at a temperature of 25 to 35 ℃ 48 to 96 It is prepared by fermentation for a period of time.

At this time, preferably, the monoginasansa leaves, kava kava roots, and red beans are mixed and extracted in a weight ratio of 0.1 to 0.5: 0.5 to 1: 0.5 to 1, more preferably a weight ratio of 0.5: 1: 1 are mixed and extracted.

In the preparation of the mixed extract, selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether At least one solvent may be used.

In the present invention, the mixed fermented extract as an active ingredient is prepared by first fermenting a mixture of monoginasansa leaf, kava kava root and red bean mixture at a temperature of 25 to 35 ° C. for 48 to 96 hours using lactic acid bacteria, and then extracting with a solvent. may be

According to a preferred embodiment of the present invention, the mixed fermented extract is (A) preparing a mixed extract by adding purified water to a mixture of monogina cranberry leaves, kava kava roots and red beans and extracting at 90 ° C. for 1 hour; (B) inoculating the mixed extract with Lactobacillus plantarum as a lactic acid bacterium, and fermenting at a temperature of 25 to 35 ° C. for 48 to 96 hours; And (C) is prepared by a manufacturing method comprising the step of filtering and drying.

In the step (A), the mixture is made by mixing monoginasansa leaves, kava kava roots, and red beans in a weight ratio of 0.1 to 0.5: 0.5 to 1: 0.5 to 1, respectively, in a weight ratio of 0.5: 1: 1, respectively. When mixed and extracted, it is more preferable because it shows a more excellent effect.

The mixed fermented extract of natural substances of the present invention prepared as described above showed excellent hydrogen peroxide inhibitory activity (Test Example 1) and melanin content increasing activity (Test Examples 3 and 4), and excellent scalp protection effect (Test Example 2) and hair protection effect. (Test Example 5) was shown.

The mixed fermented extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition for hair can be usefully used as a cosmetic for improving gray hair or protecting hair and scalp.

The composition of the present invention, in addition to the mixed extract, is a conventional cosmetic or dermatological field such as a fatty substance, surfactant, preservative, vitamin, wetting agent, water, emulsifier, essential oil or any other ingredient commonly used in cosmetics. It may contain adjuvants used as In addition, the above components may be introduced in an amount generally used in the field of skin science.

The formulation of the composition of the present invention is not limited, but can be used as an adjuvant for synthetic material dyes, and can be prepared in the form of general emulsified formulations and solubilized formulations. Emulsified cosmetics include shampoo, conditioner, hair essence, etc., and solubilized cosmetics include hair tonic and hair packs.

In addition, by containing a dermatologically acceptable medium and base other than cosmetics, it can be prepared in the form of an adjuvant that can be applied topically and systemically, which is commonly used in the field of dermatology.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited by the following examples.

Comparative Examples 1 to 3: Preparation of mixed extracts of natural substances

After mixing monoginasansa leaves, kava cava roots, and red beans in the ratio shown in Table 1 below, they were put in purified water corresponding to 10 times the dry weight and extracted at 90 ° C. for 1 hour. This was concentrated at a temperature of 50 to 60 ° C using a vacuum concentrator and then freeze-dried with a lyophilizer to obtain an extracted powder.

Examples 1 to 3: Preparation of mixed fermented extracts of natural substances

After mixing monoginasansa leaves, kava cava roots, and red beans in the ratio shown in Table 1 below, purified water corresponding to 10 times the dry weight was added and extracted at 90 ° C. for 1 hour. The mixed extract was inoculated with Lactobacillus plantarum, and fermented for 60 hours at a temperature of 30°C. After filtration, the mixture was concentrated at a temperature of 50 to 60° C. using a vacuum concentrator, and then freeze-dried with a lyophilizer to obtain an extracted powder.

weight ratio monoginasansa leaves kava kava root red bean Comparative Example 1 - One One Comparative Example 2 0.1 One One Comparative Example 3 0.5 One One Example 1 0.5 One One Example 2 0.1 One One Example 3 - One One

Test Example 1: Hydrogen peroxide scavenging ability

The hydrogen peroxide (H₂O₂) scavenging activity of the extracts according to the comparative examples and examples was measured. 100 μL of each of the above samples was put into a 96 microwell plate. 20 μL of hydrogen peroxide was added thereto and reacted for 5 minutes in an incubator at 37°C. After the reaction was over, 30 μl of each of 1.25 mM ABTS and peroxidase (1 unit/mL) was added, and finally the reaction was performed in an incubator at 37° C. for 10 minutes, and the absorbance was measured at 405 nm using an ELISA reader. The results are shown in Table 2 below. The control, 0.1% BHT, showed 84%, and the samples of Examples 1 and 2 showed higher hydrogen peroxide scavenging activity than the control. In particular, in Example 1, a remarkably high scavenging ability was measured, and it was confirmed that the hydrogen peroxide scavenging ability was the best.

Hydrogen peroxide scavenging ability (%) Comparative Example 1 52.5 Comparative Example 2 54.8 Comparative Example 3 60.4 Example 1 90.2 Example 2 85.0 Example 3 82.4 Control 84.1

Test Example 2: H ₂ O ₂ Inhibitory effect of dermal papilla apoptosis induced by

In order to confirm the effect of inhibiting cytotoxicity caused by H ₂ O ₂ , samples were prepared by diluting Comparative Examples 1 to 3 and Examples 1 to 3 with DMSO for sterilization.

Human Follicle Dermal Papilla Cells (HPDPC) were inoculated in a 6-well plate at 1×10 ⁵ cells/well and cultured for 24 hours. Here, H ₂ O ₂ 3mM and the prepared sample were added according to concentration, followed by incubation for 24 hours, and then 10 μL of MTT solution (5 mg/mL) was added. After 4 hours, the medium was discarded, DMSO was added to dissolve formazan, and absorbance was measured using an ELISA reader at 570 nm. The results are shown in Table 3 below.

Cytotoxic inhibition ability (%) Comparative Example 1 58.7 Comparative Example 2 61.2 Comparative Example 3 62.9 Example 1 91.3 Example 2 88.6 Example 3 84.2

As a result of confirming cell death caused by H ₂ O ₂ through MTT assay, it was confirmed that the lactic acid bacteria fermentation mixture extract, Examples 1, 2, and 3, had a higher cell death inhibitory effect.

Test Example 3: Melanin production measurement test

Melanin is biosynthesized in the melanosome, an organelle within the melanocyte present in the basal layer of the epidermis, and makes the hair look black, which can be compared by measuring the absorbance in the visible light range. Melanoma cell B16F10 was inoculated in a 96-well plate at a concentration of 1.0×10 ⁶ cells/well, and the samples of Comparative Examples 1 to 3 and Examples 1 to 3 were treated and cultured for 3 days. After culturing, the medium of each well was removed, washed with PBS, and 1 mL of 1 N NaOH was added to each well, followed by stirring to elute the melanin component. In order to measure the content of eluted melanin, the produced melanin content was confirmed by measuring the absorbance at 450 nm using a microplate reader, and the results are shown in Table 4.

Amount of melanin produced (%) = (Amount of production in the sample treated group / Amount of production in the untreated group) × 100

Melanin production (%) Comparative Example 1 101.6±3.5 Comparative Example 2 105.1±4.2 Comparative Example 3 107.2±5.6 Example 1 134.7±2.1 Example 2 128.1±1.3 Example 3 120.7±6.6

As confirmed in Table 4, as a result of measuring the amount of malanin production in Comparative Examples 1 to 3 and Examples 1 to 3, a high melanin production amount was shown in Examples 1 to 3 compared to Comparative Examples 1 to 3, Example 1 , 2 showed a higher production amount, and it was confirmed that the mixed fermented extract of monogina sansa leaf, kava kava root, and red bean prepared through the lactic acid bacteria fermentation process was effective as a gray hair improvement material.

Test Example 4: Tyrosinase activity promoting effect

Tyrosinase is a major enzyme involved in melanin production, and it is known that pigment expression is regulated by controlling the activity of tyrosinase. Intracellular tyrosinase activity analysis can be measured and compared by colorimetric method of DOPA quinone produced as a result of the action of tyrosinase present in cells.

B16 melanoma cells were divided into 5×10 ⁵ cells/well in a 6-well plate, cultured for 5 h, and then Comparative Examples 1 to 3 and Examples 1 to 3 were treated by concentration. After culturing for 72 h, the pellet obtained by trypsinization was dissolved in a PBS solution containing Triton X-100. After mixing equal amounts of each protein with 0.1M potassium phosphate buffer (pH 6.8) supplemented with 10 mM L-DOPA, incubated for 20 min in a 37° C. incubator, and absorbance was measured at 490 nm with a microplate reader. The results are shown in Table 5 below.

Tyrosinase activity increase rate (%) = (absorbance of sample-added group - absorbance of sample-free group) / absorbance of sample-free group × 100

Increase in tyrosinase activity (%) Comparative Example 1 9.4±5.1 Comparative Example 2 11.6±1.3 Comparative Example 3 13.1±6.4 Example 1 20.1±3.5 Example 2 18.9±4.6 Example 3 17.3±2.9

As a result of confirming intracellular tyrosinase activity analysis, compared to Comparative Examples 1-3, Examples 1-3 showed higher activity.

Test Example 5: Hair protection effect

In order to confirm the effect of increasing the amount of hair moisture of the natural product fermented extract of Example, 5g of normal hair was bundled to make a tress, and 1% of each sample was applied to damaged dry hair washed and dried after bleaching for 30 minutes with a bleaching agent. After that, it was placed in a plastic pouch and left at room temperature for 1 hour by minimizing exposure to external air. The moisture content of the hair was measured by heating at 180° C. for 10 minutes to measure the amount of moisture evaporation (%, w/w), and the results are shown in Table 6 below.

division Total moisture content of damaged hair (%) Comparative Example 1 16.9 Comparative Example 2 18.1 Comparative Example 3 19.3 Example 1 22.0 Example 2 21.7 Example 3 20.9 Distilled water 14.3

As confirmed in Table 6, it was confirmed that the mixed fermented extract of Examples of the present invention further increased the moisture content of damaged dry hair compared to the mixed extract of Comparative Example.

Claims (7)

A cosmetic composition for hair for improving gray hair, containing as an active ingredient a mixed fermented extract of monoginasansansa leaf, kava cava root, and red bean mixture prepared by fermenting the mixed extract of monoginasansansa leaf, kava cava root, and red bean with lactic acid bacteria. delete The method of claim 1, wherein the lactic acid bacteria are Lactobacillus plantarum, Lactobacillus lactis, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Pediococcus serei Pediococcus cerevisiae, Lactobacillus breve, Leuconostoc mesenteroides, Bifidobacterium bifidum, Lactobacillus sakei and Lactobacillus crispa A cosmetic composition for hair for improving gray hair, characterized in that it is at least one selected from the group consisting of tooth (Lactobacillus crispatus). The hair cosmetic for improving gray hair according to claim 1, wherein the mixed extract is obtained by mixing and extracting monogina hawthorn leaves, kava kava roots, and red beans in a weight ratio of 0.1 to 0.5:0.5 to 1:0.5 to 1, respectively. composition. The cosmetic composition for hair for improving gray hair according to claim 1, wherein the mixed fermented extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition. delete The cosmetic composition for hair for improving gray hair according to claim 1, wherein the cosmetic composition for hair has an effect of protecting hair and scalp.