KR20210119680A - Composition for enhancing skin barrier comprising eggshell membrane powder - Google Patents
Composition for enhancing skin barrier comprising eggshell membrane powder Download PDFInfo
- Publication number
- KR20210119680A KR20210119680A KR1020200036079A KR20200036079A KR20210119680A KR 20210119680 A KR20210119680 A KR 20210119680A KR 1020200036079 A KR1020200036079 A KR 1020200036079A KR 20200036079 A KR20200036079 A KR 20200036079A KR 20210119680 A KR20210119680 A KR 20210119680A
- Authority
- KR
- South Korea
- Prior art keywords
- strengthening
- skin barrier
- protecting
- eggshell membrane
- powder
- Prior art date
Links
- 229940012466 egg shell membrane Drugs 0.000 title claims abstract description 116
- 239000000843 powder Substances 0.000 title claims abstract description 80
- 239000000203 mixture Substances 0.000 title claims abstract description 69
- 230000008591 skin barrier function Effects 0.000 title claims abstract description 49
- 230000002708 enhancing effect Effects 0.000 title abstract 3
- 238000005728 strengthening Methods 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 210000003278 egg shell Anatomy 0.000 claims description 30
- 102000002322 Egg Proteins Human genes 0.000 claims description 28
- 108010000912 Egg Proteins Proteins 0.000 claims description 28
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 18
- 235000013376 functional food Nutrition 0.000 claims description 18
- 230000036541 health Effects 0.000 claims description 15
- -1 pack Substances 0.000 claims description 15
- 235000013305 food Nutrition 0.000 claims description 12
- 239000002537 cosmetic Substances 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 241000195493 Cryptophyta Species 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 9
- 239000000839 emulsion Substances 0.000 claims description 8
- 238000010298 pulverizing process Methods 0.000 claims description 8
- 235000013361 beverage Nutrition 0.000 claims description 7
- 239000006210 lotion Substances 0.000 claims description 7
- 239000006071 cream Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000006072 paste Substances 0.000 claims description 5
- 239000000344 soap Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 239000000428 dust Substances 0.000 claims description 4
- 239000000499 gel Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000002674 ointment Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 239000002585 base Substances 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 239000006188 syrup Substances 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 2
- 235000009508 confectionery Nutrition 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000000686 essence Substances 0.000 claims description 2
- 239000006260 foam Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 239000008234 soft water Substances 0.000 claims description 2
- 230000000475 sunscreen effect Effects 0.000 claims description 2
- 239000000516 sunscreening agent Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 239000013040 bath agent Substances 0.000 claims 1
- 210000003491 skin Anatomy 0.000 abstract description 41
- 229940106189 ceramide Drugs 0.000 abstract description 18
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 abstract description 15
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 abstract description 15
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 abstract description 15
- 230000001965 increasing effect Effects 0.000 abstract description 15
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 abstract description 15
- 210000002615 epidermis Anatomy 0.000 abstract description 11
- 238000010171 animal model Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- 239000004480 active ingredient Substances 0.000 abstract description 6
- 230000036572 transepidermal water loss Effects 0.000 abstract description 3
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 27
- 238000012360 testing method Methods 0.000 description 16
- 230000037380 skin damage Effects 0.000 description 12
- 238000001704 evaporation Methods 0.000 description 11
- 230000008020 evaporation Effects 0.000 description 11
- 238000010172 mouse model Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 239000000047 product Substances 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 235000014113 dietary fatty acids Nutrition 0.000 description 8
- 239000000194 fatty acid Substances 0.000 description 8
- 229930195729 fatty acid Natural products 0.000 description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 7
- 206010060891 General symptom Diseases 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 238000000692 Student's t-test Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 150000002632 lipids Chemical class 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 239000003921 oil Substances 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 230000001681 protective effect Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 150000001783 ceramides Chemical class 0.000 description 3
- 230000002550 fecal effect Effects 0.000 description 3
- 235000013373 food additive Nutrition 0.000 description 3
- 239000002778 food additive Substances 0.000 description 3
- 235000015203 fruit juice Nutrition 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 235000012149 noodles Nutrition 0.000 description 3
- 238000001543 one-way ANOVA Methods 0.000 description 3
- 239000000546 pharmaceutical excipient Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 210000000434 stratum corneum Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 description 3
- 239000008158 vegetable oil Substances 0.000 description 3
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 239000012981 Hank's balanced salt solution Substances 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000004166 Lanolin Substances 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 239000000440 bentonite Substances 0.000 description 2
- 229910000278 bentonite Inorganic materials 0.000 description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 2
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 235000005687 corn oil Nutrition 0.000 description 2
- 239000002285 corn oil Substances 0.000 description 2
- 210000004207 dermis Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000013355 food flavoring agent Nutrition 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 235000019388 lanolin Nutrition 0.000 description 2
- 229940039717 lanolin Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000021067 refined food Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000003765 sweetening agent Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- WNWHHMBRJJOGFJ-UHFFFAOYSA-N 16-methylheptadecan-1-ol Chemical class CC(C)CCCCCCCCCCCCCCCO WNWHHMBRJJOGFJ-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 206010012438 Dermatitis atopic Diseases 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 238000011804 SKH1 hairless mouse Methods 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 102000012883 Tumor Necrosis Factor Ligand Superfamily Member 14 Human genes 0.000 description 1
- 108010065158 Tumor Necrosis Factor Ligand Superfamily Member 14 Proteins 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- 229960002903 benzyl benzoate Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000013574 canned fruits Nutrition 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 210000000991 chicken egg Anatomy 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000000736 corneocyte Anatomy 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 108010007093 dispase Proteins 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 235000013611 frozen food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical class C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 235000013310 margarine Nutrition 0.000 description 1
- 239000003264 margarine Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000021096 natural sweeteners Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 230000037070 skin defense Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/40—Shell-fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Marine Sciences & Fisheries (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
본 발명은 난각막 분말을 포함하는 피부장벽 강화용 조성물에 관한 것으로, 구체적으로 난각막 분말을 포함하는 미세먼지 또는 자외선 등에 대한 피부장벽 강화 또는 보호용 조성물에 관한 것이다.The present invention relates to a skin barrier strengthening composition comprising eggshell membrane powder, and more particularly, to a skin barrier strengthening or protecting composition against fine dust or ultraviolet rays, including the eggshell membrane powder.
최근 환경의 변화나 생활 패턴의 변화에 따른 냉/난방의 인위적인 온도 조절, 사회생활에서 발생되는 각종 스트레스와 환경오염, 예컨대 미세먼지 또는 자외선으로 인한 피부 스트레스, 화장 습관에 따른 잦은 세안 및 연령 증가에 따른 자연적인 피부 노화 등의 여러 가지 원인으로 인하여 각질층의 수분이 감소하여 피부가 건조해지고 표면이 거칠게 되며 피부가 푸석거리고 촉촉함을 잃어 생기가 없어 보이는 등의 현상이 발생하기 때문에 피부 장벽 강화 또는 보호용 조성물의 필요가 증가하고 있다.Artificial temperature control of cooling/heating according to recent environmental changes or changes in living patterns, various stresses and environmental pollution occurring in social life, such as skin stress caused by fine dust or UV rays, frequent washing due to makeup habits, and increasing age Due to various causes such as natural skin aging, the moisture in the stratum corneum decreases, the skin becomes dry, the surface becomes rough, and the skin becomes brittle and loses moisture and looks lifeless. Therefore, a composition for strengthening or protecting the skin barrier the need for is increasing.
피부는 조직학적으로 표피(epidermis), 진피(dermis), 피하지방(subcutis)의 3개의 층으로 구성되어 있는데, 이중 가장 외부에 존재하는 표피는 피부노화의 측면에서 가장 중요한 역할을 하며 피부미용면에서도 가장 집중적인 연구의 대상이 되고 있다. 표피에서도 최외각층인 각질층을 구성하는 성분은 각질세포와 피부지질이다. 피부지질은 피부의 장벽 기능을 담당하며 유해물질로부터 피부를 보호, 체내 물질의 유출 방지 및 피부 수분 증발을 방지하는 중요한 기능을 한다.The skin is histologically composed of three layers: the epidermis, the dermis, and the subcutis. It is also the subject of the most intensive research. The components that make up the stratum corneum, the outermost layer of the epidermis, are keratinocytes and skin lipids. Skin lipids are responsible for the barrier function of the skin, and play an important role in protecting the skin from harmful substances, preventing the leakage of substances in the body, and preventing the evaporation of moisture from the skin.
피부지질은 각질세포(corneocytes) 간의 공간을 채우고 있으며 주로 세라마이드(40~65%), 콜레스테롤(10%) 및 유리지방산(25%)으로 구성되어 있다(Human Skin-identical Ceramides 2000; 한국미용학회지, 2002). 이러한 지질들은 각질세포 사이에 존재하여 수분의 증발을 방지하고, 외부 자극이나 오염으로부터 피부를 보호하는 피부장벽의 기능을 수행한다.Skin lipids fill the space between corneocytes and are mainly composed of ceramides (40-65%), cholesterol (10%) and free fatty acids (25%) (Human Skin-identical Ceramides 2000; Journal of the Korean Society of Aesthetics, 2002). These lipids exist between keratinocytes to prevent evaporation of moisture and function as a skin barrier to protect the skin from external stimuli or contamination.
이중 세라마이드는 피부지질의 주성분으로서 함량이 감소하게 되면, 수분증발이 증가하고 아토피 등 다양한 피부질환이 악화되며 피부의 노화가 진행되지만 외부로부터 세라마이드를 보충하면 피부를 정상상태로 회복시킬 수 있는 것으로 알려져 있다.Of these, ceramide is the main component of skin lipids, and when the content decreases, moisture evaporation increases, various skin diseases such as atopic dermatitis worsen, and skin aging progresses. have.
한편, 난각막은 계란과 같은 조류(鳥類) 알의 속껍질로서 주로 알의 내용물인 난백과 난황을 사용하는 과정에서 부산물로 발생한다. 이때 발생한 부산물은 겉껍질(난각)과 속껍질(난각막 또는 난막)이 붙은 상태로 이전에는 부가가치가 거의 없어 가공 비용을 충당하면서 산업적으로 폐기하였다. 최근 칼슘이 주성분인 겉껍질은 분리하여 식품용이나 사료용으로 사용한다. 이때 겉껍질에서 약 4~5%를 차지하는 분리된 속껍질이 난각막이다.On the other hand, the eggshell membrane is the inner shell of algae eggs such as eggs, and is mainly generated as a by-product in the process of using egg white and yolk, which are the contents of eggs. The by-products generated at this time were industrially discarded to cover the processing cost as they had little added value before in a state in which the outer shell (egg shell) and the inner shell (egg shell membrane or egg membrane) were attached. Recently, the outer shell, which contains calcium as the main ingredient, is separated and used for food or feed. At this time, the separated inner shell, which accounts for about 4-5% of the outer shell, is the eggshell membrane.
일반적으로 난각막은 수분을 일정량 함유하고 있으며, 수분을 제외한 순수한 고형분의 함량은 5 내지 30 중량%를 차지한다. 이 난각막은 생체형성으로 두께 0.01 내지 0.02 mm의 해면체 구조의 섬유상으로 구성되며, 주요 아미노산은 히스티딘(Histidine)과 시스테인(Cysteine), 프롤린 (Proline) 등의 케라틴과 콜라겐 등으로 분자량이 약 10만 이상의 섬유상 구조 단백질 또는 경단백질이다. 이처럼 난각막 성분에 대해 연구가 이루어지면서 최근 난각막을 다양한 산업에 적용하려는 시도가 이루어지고 있다.In general, the eggshell membrane contains a certain amount of moisture, and the content of pure solids excluding moisture accounts for 5 to 30% by weight. This eggshell membrane is bioformed and is composed of fibrous spongy bodies with a thickness of 0.01 to 0.02 mm. The main amino acids are keratin such as histidine, cysteine, and proline, and collagen, etc. with a molecular weight of about 100,000 or more. It is a fibrous structural protein or a light protein. As such, research on the components of eggshell membranes has led to recent attempts to apply eggshell membranes to various industries.
이에, 본 발명자들은 부작용 문제를 유발하지 않고 원료 확보가 용이한 피부 장벽 강화 또는 보호용 천연 소재를 개발하기 위해 노력한 결과, 자외선 조사로 유도한 피부손상 동물모델에 경구투여된 난각막 분말이 피부표면의 수분을 증가시키고, 경피수분손실을 억제하며, 표피층의 세라마이드를 증가시키는 효과를 나타냄을 확인하여, 상기 난각막 분말을 피부장벽 강화 및 보호용 조성물의 유효성분으로 이용할 수 있음을 밝힘으로써, 본 발명을 완성하였다.Accordingly, the present inventors have made efforts to develop a natural material for strengthening or protecting the skin barrier that does not cause side effects and is easy to secure raw materials. The present invention was completed by confirming that the eggshell membrane powder can be used as an active ingredient in a composition for strengthening and protecting the skin barrier by increasing .
본 발명의 목적은 난각막 분말을 포함하는, 피부장벽 강화 또는 보호용 조성물을 제공하는 것이다.An object of the present invention is to provide a composition for strengthening or protecting the skin barrier, comprising eggshell membrane powder.
상기 목적을 달성하기 위하여, 본 발명은 난각막 분말을 포함하는, 피부장벽 강화 또는 보호용 건강기능식품 조성물을 제공한다.In order to achieve the above object, the present invention provides a health functional food composition for strengthening or protecting the skin barrier, comprising eggshell membrane powder.
또한, 본 발명은 난각막 분말을 포함하는, 피부장벽 강화 또는 보호용 식품 조성물을 제공한다.In addition, the present invention provides a food composition for strengthening or protecting the skin barrier, comprising eggshell membrane powder.
또한, 본 발명은 난각막 함유 분말을 포함하는 피부장벽 강화 또는 보호용 화장료 조성물을 제공한다.In addition, the present invention provides a cosmetic composition for strengthening or protecting the skin barrier comprising the eggshell membrane-containing powder.
아울러, 본 발명은 난각막 함유 분말을 포함하는 피부장벽 강화 또는 보호용 피부외용제 조성물을 제공한다.In addition, the present invention provides a composition for external application for skin for strengthening or protecting the skin barrier comprising the eggshell membrane-containing powder.
본 발명의 난각막 분말은 자외선 조사로 유도한 피부손상 동물모델에서 피부표면의 수분을 증가시키고, 경피수분손실을 억제하며, 표피층의 세라마이드를 증가시키는 효과를 나타내므로, 피부장벽 강화 및 보호용 조성물의 유효성분으로 이용할 수 있다.The eggshell membrane powder of the present invention increases the moisture on the skin surface in an animal model of skin damage induced by UV irradiation, suppresses transdermal moisture loss, and increases the ceramide of the epidermal layer, so the skin barrier strengthening and protective composition is effective It can be used as an ingredient.
도 1은 본 발명의 일 실시예에 따라 제조한 난각막 분말을 자외선 조사로 유도한 피부손상 동물모델에 경구투여하는 12주 동안 체중변화를 확인한 도이다.
도 2는 본 발명의 일 실시예에 따라 제조한 난각막 분말을 자외선 조사로 유도한 피부손상 동물모델에 경구투여 12주 동안(위) 및 12주 차에(아래) 피부표면 수분함량을 확인한 도이다:
$$: G1 대비 유의차(Significant difference, student's t-test, p<0.01);
##: G2 대비 유의차(student's t-test, p<0.01); 및
**: G2 대비 유의차(one-way ANOVA, p<0.01).
도 3은 본 발명의 일 실시예에 따라 제조한 난각막 분말을 자외선 조사로 유도한 피부손상 동물모델에 경구투여 12주 동안(위) 및 12주 차에(아래) 피부 경피수분증발량을 확인한 도이다:
$$: G1 대비 유의차(Significant difference, student's t-test, p<0.01);
##: G2 대비 유의차(student's t-test, p<0.01); 및
**: G2 대비 유의차(one-way ANOVA, p<0.01).
도 4는 본 발명의 일 실시예에 따라 제조한 난각막 분말을 자외선 조사로 유도한 피부손상 동물모델에 경구투여 12주 차에 피부 조직에서 세라마이드(Ceramide) 함량을 확인한 도이다:
$$: G1 대비 유의차(Significant difference, student's t-test, p<0.01);
##: G2 대비 유의차(student's t-test, p<0.01); 및
**: G2 대비 유의차(one-way ANOVA, p<0.01).1 is a diagram illustrating changes in body weight during 12 weeks of oral administration of an eggshell membrane powder prepared according to an embodiment of the present invention to an animal model with skin damage induced by UV irradiation.
2 is a diagram illustrating the skin surface moisture content during 12 weeks (top) and 12 weeks (bottom) after oral administration of an egg shell membrane powder prepared according to an embodiment of the present invention to an animal model with skin damage induced by UV irradiation. :
$$: Significant difference compared to G1 (student's t-test, p<0.01);
##: significant difference compared to G2 (student's t-test, p<0.01); and
**: Significant difference compared to G2 (one-way ANOVA, p<0.01).
3 is a diagram confirming the amount of skin percutaneous moisture evaporation during 12 weeks (top) and 12 weeks (bottom) after oral administration of an eggshell membrane powder prepared according to an embodiment of the present invention to a skin-damaged animal model induced by UV irradiation. :
$$: Significant difference compared to G1 (student's t-test, p<0.01);
##: significant difference compared to G2 (student's t-test, p<0.01); and
**: Significant difference compared to G2 (one-way ANOVA, p<0.01).
4 is a diagram illustrating the content of ceramide in skin tissue at the 12th week of oral administration to an animal model with skin damage induced by UV irradiation of eggshell membrane powder prepared according to an embodiment of the present invention:
$$: Significant difference compared to G1 (student's t-test, p<0.01);
##: significant difference compared to G2 (student's t-test, p<0.01); and
**: Significant difference compared to G2 (one-way ANOVA, p<0.01).
이하 본 발명을 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 난각막(eggshell membrane) 분말을 포함하는, 피부장벽 강화 또는 보호용 건강기능식품 조성물을 제공한다.The present invention provides a health functional food composition for strengthening or protecting the skin barrier, comprising eggshell membrane powder.
또한, 본 발명은 난각막 분말을 포함하는, 피부장벽 강화 또는 보호용 식품 조성물을 제공한다.In addition, the present invention provides a food composition for strengthening or protecting the skin barrier, comprising eggshell membrane powder.
본 발명에서, 상기 난각막은 인공적 처리를 가하지 않고 폐기되는 상태 그대로가 사용될 수 있고, 또는 인공적 처리를 가한 것이 사용될 수도 있다.In the present invention, the egg shell membrane may be used as it is discarded without artificial treatment, or artificial treatment may be used.
구체적으로, 상기 난각막 분말은 하기 단계를 거쳐 제조될 수 있다:Specifically, the eggshell membrane powder may be prepared through the following steps:
1) 조류의 알 껍질(eggshell)과 물을 1 : 0.5 내지 10의 중량비로 혼합하고 물로 세척한 후 분쇄하여 분쇄물을 획득하는 단계;1) mixing eggshells of algae with water in a weight ratio of 1: 0.5 to 10, washing with water, and pulverizing to obtain a pulverized product;
2) 획득한 분쇄물에 물을 첨가하고 교반하여 난각막을 획득하는 단계;2) adding water to the obtained pulverized product and stirring to obtain an eggshell membrane;
3) 획득한 난각막을 증기로 열처리하여 1차 살균하는 단계;3) primary sterilization by heat-treating the obtained eggshell membrane with steam;
4) 1차 살균한 난각막에 염산 용액을 넣고 pH 3.0 내지 4.5에서 가열하여 2차 살균하는 단계; 4) adding a hydrochloric acid solution to the first sterilized eggshell membrane and heating at pH 3.0 to 4.5 for secondary sterilization;
5) 2차 살균한 난각막을 원심분리하여 염산 용액을 제거한 후 건조하여 난각막 건조물을 획득하는 단계; 및5) centrifuging the second sterilized egg shell membrane to remove the hydrochloric acid solution and drying it to obtain a dried egg shell membrane; and
6) 획득한 난각막 건조물을 분말화하여 난각막 분말을 획득하는 단계.6) pulverizing the obtained eggshell membrane powder to obtain eggshell membrane powder.
상기 단계 1)은 조류의 알 껍질을 물로 세척한 후 분쇄하여 분쇄물을 획득하는 단계로, 상기 세척을 통해 조류의 알 껍질을 물로 세척하여 껍질에 존재하는 분변 미생물을 제거할 수 있다. 상기 세척은 구체적으로 조류의 알 껍질과 물을 1 : 0.5 내지 10의 중량비로 혼합하고 물로 세척할 수 있고, 보다 구체적으로 1 : 1 내지 5의 중량비로 혼합하고 물로 세척할 수 있으며, 보다 더 구체적으로 1 : 2 내지 4의 중량비로 혼합하고 물로 세척할 수 있다. 세척을 위한 조류의 알 껍질과 물의 혼합비율이 상기 하한치 미만인 경우에는 난각에 분변 미생물이 다량 존재하여 이후 살균공정으로도 일부 미생물이 멸균되지 않을 수 있다.Step 1) is a step of obtaining a pulverized product by washing the eggshell of the algae with water and then pulverizing it. Through the washing, the eggshell of the algae is washed with water to remove fecal microorganisms present in the shell. The washing can be specifically performed by mixing the egg shell of algae with water in a weight ratio of 1: 0.5 to 10 and washing with water, more specifically, mixing in a weight ratio of 1: 1 to 5 and washing with water, and more specifically It can be mixed with 1: 2 to 4 weight ratio and washed with water. When the mixing ratio of the eggshell and water of the algae for washing is less than the lower limit, a large amount of fecal microorganisms are present in the eggshell, and some microorganisms may not be sterilized even after the sterilization process.
또한, 상기 분쇄를 통해 세척한 조류의 알 껍질을 작은 조각으로 분할하고 알 껍질로부터 난각과 난각막이 분리되도록 함으로써, 난각과 난각으로부터 분리된 난각막으로 구성된 분쇄물을 획득할 수 있다. 상기 분쇄는 구체적으로 햄머밀(Hammer mill) 또는 햄머크러셔(Hammer crusher)를 이용하여 10 내지 30분 동안 수행할 수 있고, 보다 구체적으로 15 내지 25분 동안 수행할 수 있다. 분쇄 시간이 상기 범위를 벗어난 경우에는 난각으로부터 분리되지 않은 난각막이 다량 존재하여 난각막의 수득율이 떨어질 수 있다.In addition, by dividing the washed algae eggshell into small pieces and allowing the eggshell and the eggshell membrane to be separated from the eggshell, a pulverized product composed of the eggshell and the eggshell membrane separated from the eggshell can be obtained. The pulverization may be specifically performed for 10 to 30 minutes using a hammer mill or a hammer crusher, and more specifically, may be performed for 15 to 25 minutes. If the grinding time is out of the above range, a large amount of eggshell membrane not separated from the eggshell may exist, and thus the yield of the eggshell membrane may decrease.
또한, 상기 조류의 알 껍질은 가금류, 예컨대 닭, 메추리, 오리 또는 거위의 알 껍질일 수 있으나, 이에 제한되는 것은 아니다.In addition, the egg shell of the bird may be the egg shell of poultry, such as chicken, quail, duck or goose, but is not limited thereto.
상기 단계 2)는 단계 1)에서 획득한 분쇄물에 물을 첨가하고 교반하여 난각막을 획득하는 단계로, 난각과 난각으로부터 분리된 난각막으로 구성된 분쇄물에서 난각막을 획득하는 단계이다. 구체적으로 분쇄물에 물을 1 : 1 내지 5의 중량비, 보다 더 구체적으로 1 : 2 내지 4의 중량비로 첨가하고 교반하면 분쇄물에 포함된 난각은 바닥으로 가라앉고, 난각막은 물 위로 떠오르게 되어 떠오른 난각막을 획득할 수 있다.Step 2) is a step of obtaining an eggshell membrane by adding water to the pulverized product obtained in step 1) and stirring it, and is a step of obtaining an eggshell membrane from a pulverized product composed of an eggshell and an eggshell membrane separated from the eggshell. Specifically, when water is added to the pulverized product in a weight ratio of 1: 1 to 5, more specifically, 1: 2 to 4 by weight, and stirred, the egg shells contained in the pulverized product sink to the bottom, and the egg shell membrane floats to the top of the water. You can obtain an eggshell membrane.
상기 단계 3)은 획득한 난각막을 증기로 열처리하여 1차 살균하는 단계로, 구체적으로 난각막을 65 내지 95℃의 증기로 30 내지 60분 동안 열처리할 수 있고, 보다 구체적으로 75 내지 85℃의 증기로 40 내지 50분 동안 열처리할 수 있다. 1차 살균의 온도 및 시간이 상기 하한치 미만인 경우에는 난각막에 일부 존재하는 미생물이 완전히 멸균되지 않을 수 있다. 또한 상기 상한치 초과인 경우에는 난각막에 포함된 구성 성분, 예컨대 단백질, 콜라겐 등이 변성 또는 파괴되어 효능이 떨어질 수 있다.Step 3) is a step of primary sterilization by heat-treating the obtained eggshell membrane with steam. Specifically, the eggshell membrane may be heat-treated with steam at 65 to 95° C. for 30 to 60 minutes, and more specifically, steam at 75 to 85° C. It can be heat-treated for 40 to 50 minutes. If the temperature and time of the primary sterilization are less than the lower limit, some microorganisms present in the eggshell membrane may not be completely sterilized. In addition, if the upper limit is exceeded, the components included in the eggshell membrane, such as proteins, collagen, etc. may be denatured or destroyed, and thus the efficacy may be reduced.
상기 단계 4)는 1차 살균한 난각막을 산성 조건에서 열처리하여 2차 살균하는 단계로, 구체적으로 1차 살균한 난각막에 염산 용액을 넣고 pH 3.0 내지 4.5에서 가열할 수 있고, 보다 구체적으로 pH 3.0 내지 4.5에서 65 내지 95℃ 온도로 20 내지 40분 동안 가열할 수 있으며, 보다 더 구체적으로 pH 3.0 내지 4.5에서 75 내지 85℃ 온도로 25 내지 35분 동안 가열할 수 있다. 2차 살균의 pH, 온도 및 시간이 상기 하한치 미만인 경우에는 1차 살균 이후 남아있는 미생물이 완전히 멸균되지 않을 수 있다. 또한 상기 상한치 초과인 경우에는 난각막에 포함된 구성 성분이 변성 또는 파괴되어 효능이 떨어질 수 있다.Step 4) is a step of secondary sterilization by heat-treating the primary sterilized egg shell membrane under acidic conditions. Specifically, hydrochloric acid solution may be added to the primary sterilized egg shell membrane and heated at pH 3.0 to 4.5, and more specifically, pH 3.0 It can be heated to a temperature of 65 to 95 ° C. at 4.5 to 20 to 40 minutes, and more specifically, heated to a temperature of 75 to 85 ° C. at pH 3.0 to 4.5 for 25 to 35 minutes. If the pH, temperature, and time of the secondary sterilization are less than the lower limit, the microorganisms remaining after the primary sterilization may not be completely sterilized. In addition, if the upper limit is exceeded, the components included in the eggshell membrane may be denatured or destroyed, and thus the efficacy may be reduced.
상기 단계 5)는 2차 살균한 난각막에서 염산 용액을 제거하는 단계로, 2차 살균한 난각막을 원심분리하여 염산 용액을 제거하고 건조하여 난각막 건조물을 획득할 수 있다.In step 5), the hydrochloric acid solution is removed from the second sterilized egg shell membrane. The second sterilized egg shell membrane is centrifuged to remove the hydrochloric acid solution and dried to obtain a dried egg shell membrane.
상기 단계 6)는 획득한 난각막 건조물을 분말화하여 난각막 분말을 획득하는 단계로, 구체적으로 햄머밀(Hammer mill)을 이용하여 체적평균입자경이 100 내지 400 ㎛, 보다 구체적으로 100 내지 300 ㎛인 난각막 분말로 분말화할 수 있다.Step 6) is a step of obtaining eggshell membrane powder by pulverizing the obtained eggshell membrane powder. Specifically, an eggshell membrane having a volume average particle diameter of 100 to 400 μm, more specifically 100 to 300 μm using a hammer mill. It can be pulverized into a powder.
본 발명에서, 상기 난각막 분말은 3 내지 12 mg/kg을 1일 기준 유효량으로 포함할 수 있고, 보다 구체적으로 4 내지 10 mg/kg을 1일 기준 유효량으로 포함할 수 있다. 상기 1일 기준 유효량은, 하기 실시예에 기재된 것과 같은 동물 실험을 통해 도출된 최적의 함유량이다. 하기 실시예를 보면, 동물 실험 시 60 mg/kg/day 내지 120 mg/kg/day 경구투여군에서 보다 우수한 효과가 나타났다. 그리고 이를 인체에 적용하는 경우, 이 기술분야에 알려진 계산식에 따라 3 내지 12 mg/kg, 보다 구체적으로 4 내지 10 mg/kg, 보다 더 구체적으로 4.8 내지 9.6 mg/kg의 유효량을 가질 수 있다(Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers, FDA, 2005). 1일 기준 유효량이 상기 범위을 벗어난 경우 피부장벽 강화 및 보호 효과가 미비할 수 있다.In the present invention, the eggshell membrane powder may contain 3 to 12 mg/kg in an effective amount per day, and more specifically, 4 to 10 mg/kg in an effective amount per day. The effective amount on a daily basis is an optimal content derived from animal experiments such as those described in Examples below. Referring to the following examples, a better effect was obtained in the oral administration group from 60 mg/kg/day to 120 mg/kg/day in animal experiments. And when it is applied to the human body, it may have an effective amount of 3 to 12 mg/kg, more specifically 4 to 10 mg/kg, and even more specifically 4.8 to 9.6 mg/kg according to a calculation formula known in the art ( Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers, FDA, 2005). If the effective amount on a daily basis is out of the above range, the skin barrier strengthening and protective effect may be insufficient.
본 발명에서, 상기 피부장벽 강화 또는 보호는 자외선 또는 미세먼지에 대한 피부장벽 강화 또는 보호일 수 있고, 보다 구체적으로 자외선에 대한 피부장벽 강화 또는 보호일 수 있다.In the present invention, the strengthening or protection of the skin barrier may be strengthening or protecting the skin barrier against ultraviolet rays or fine dust, and more specifically, strengthening or protecting the skin barrier against ultraviolet rays.
또한, 상기 자외선은 자외선 B(ultraviolet-B, UVB)일 수 있다.In addition, the ultraviolet rays may be ultraviolet B (ultraviolet-B, UVB).
본 발명의 구체적인 실시예에서, 본 발명자들은 본 발명에 따라 닭의 알 껍질을 이용하여 난각막 분말을 제조하였다.In a specific embodiment of the present invention, the present inventors prepared eggshell membrane powder using chicken egg shells according to the present invention.
또한, 본 발명자들은 상기 난각막 분말을 부형제를 이용하여 경구 투여용 조성물로 제조하고 이를 자외선 조사로 유도된 피부손상 마우스 모델에 투여하였다. 그 결과, 자외선 조사로 유도된 피부손상 마우스 모델에서는 지속적인 자외선 조사로 피부표면의 수분 감소, 경피수분증발량 증가가 나타나는 반면, 난각막 분말을 투여한 경우에서는 피부표면의 수분이 증가하고 경피수분증발량이 감소하며, 피부 조직 내 세라마이드 함량이 증가하는 것을 확인하였다.In addition, the present inventors prepared the eggshell membrane powder as a composition for oral administration using an excipient and administered it to a mouse model of skin damage induced by UV irradiation. As a result, in the mouse model of skin damage induced by UV irradiation, continuous UV irradiation showed a decrease in moisture on the skin surface and an increase in transdermal moisture evaporation. and it was confirmed that the ceramide content in the skin tissue increased.
따라서, 본 발명의 난각막 분말은 자외선 조사로 유도한 피부손상 동물모델에서 피부표면의 수분을 증가시키고, 경피수분손실을 억제하며, 표피층의 세라마이드를 증가시키는 효과를 나타내므로, 상기 난각막 분말을 피부장벽 강화 및 보호용 건강기능식품 조성물 또는 식품 첨가제를 포함한 식품 조성물의 유효성분으로 유용하게 이용할 수 있다.Therefore, the eggshell membrane powder of the present invention increases the moisture on the skin surface, suppresses the transdermal moisture loss, and increases the ceramide of the epidermal layer in an animal model of skin damage induced by UV irradiation. It can be usefully used as an active ingredient in a food composition including a health functional food composition for strengthening and protection or a food additive.
본 발명에 따른 건강기능식품 조성물은 분말, 과립, 환, 정제, 캡슐, 캔디, 시럽 및, 차, 쥬스 및 드링크 형태의 음료 등의 제형으로 제조될 수 있으나, 이에 제한되는 것은 아니다.The health functional food composition according to the present invention may be prepared in the form of powders, granules, pills, tablets, capsules, candy, syrup, and beverages in the form of tea, juice and drink, but is not limited thereto.
본 발명에 따른 식품 조성물은 기능성 식품(functional food), 영양 보조제(nutritional supplement), 건강식품(health food) 및 식품 첨가제(food additives) 등의 모든 형태를 포함한다. 상기 유형의 식품 조성물은 당 업계에 공지된 통상적인 방법에 따라 다양한 형태로 제조할 수 있다.The food composition according to the present invention includes all types of functional food, nutritional supplement, health food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.
또한, 기능성 식품으로는 음료(알콜성 음료 포함), 과실 및 그의 가공식품(예: 과일통조림, 병조림, 잼, 마아말레이드 등), 어류, 육류 및 그 가공식품(예: 햄, 소시지콘비이프 등), 빵류 및 면류(예: 우동, 메밀국수, 라면, 스파게티, 마카로니 등), 과즙, 각종 드링크, 쿠키, 엿, 유제품(예: 버터, 치이즈 등), 식용식물유지, 마아가린, 식물성 단백질, 레토르트 식품, 냉동식품, 각종 조미료(예: 된장, 간장, 소스 등) 등을 들 수 있고, 통상적인 의미에서의 기능성 식품을 모두 포함한다.In addition, functional foods include beverages (including alcoholic beverages), fruits and their processed foods (eg, canned fruit, bottled, jam, marmalade, etc.), fish, meat, and their processed foods (eg, ham, corned sausage) etc.), breads and noodles (eg udon noodles, soba noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, syrup, dairy products (eg butter, cheese, etc.), edible vegetable oils and fats, margarine, vegetable protein , retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.), and includes all functional foods in a conventional sense.
본 발명에 따른 식품 조성물이 음료 조성물인 경우, 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토오스, 슈크로오스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알코올이다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 건강식품 100중량부당 0.01 ~ 0.04중량부, 보다 구체적으로 0.02 ~ 0.03중량부의 범위에서 선택하는 것이 바람직하다.When the food composition according to the present invention is a beverage composition, it may contain various flavoring agents or natural carbohydrates as an additional component like a conventional beverage. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumartin and stevia extract or synthetic sweeteners such as saccharin and aspartame may be used. The ratio of the natural carbohydrate is preferably selected in the range of 0.01 to 0.04 parts by weight, more specifically 0.02 to 0.03 parts by weight, per 100 parts by weight of the health food of the present invention.
상기 외의 본 발명의 난각막 분말은 여러 가지 부형제, 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 본 발명의 추출물은 천연 과일 주스, 과일 주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하지 않지만 본 발명의 식품 조성물 100 중량부 당 0.001 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the above, the eggshell membrane powder of the present invention contains various excipients, nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin , alcohol, a carbonation agent used in carbonated beverages, and the like. In addition, the extract of the present invention may contain the pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not very important, but is generally selected in the range of 0.001 to 0.1 parts by weight per 100 parts by weight of the food composition of the present invention.
본 발명의 난각막 분말을 식품 첨가제로 사용할 경우, 상기 난각막 분말을 그대로 첨가하거나 농축액 형태로 제조할 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. When the eggshell membrane powder of the present invention is used as a food additive, the eggshell membrane powder may be added as it is or prepared in the form of a concentrate, and may be appropriately used according to a conventional method.
본 발명에 식품 조성물은 난각막 분말을 조성물 총 중량에 대하여 0.01 ~ 90 중량%, 보다 구체적으로 0.1 ~ 50 중량%로 함유할 수 있다.The food composition of the present invention may contain the eggshell membrane powder in an amount of 0.01 to 90% by weight, more specifically 0.1 to 50% by weight, based on the total weight of the composition.
또한, 본 발명은 난각막 함유 분말을 포함하는 피부장벽 강화 또는 보호용 화장료 조성물을 제공한다.In addition, the present invention provides a cosmetic composition for strengthening or protecting the skin barrier comprising the eggshell membrane-containing powder.
상기 난각막 분말, 피부장벽 강화 또는 보호에 대해서는 상기에서 설명한 바와 같다.The eggshell membrane powder, strengthening or protecting the skin barrier is the same as described above.
또한, 본 발명의 난각막 분말은 자외선 조사로 유도한 피부손상 동물모델에서 피부표면의 수분을 증가시키고, 경피수분손실을 억제하며, 표피층의 세라마이드를 증가시키는 효과를 나타내므로, 상기 난각막 분말을 피부장벽 강화 및 보호용 화장료 조성물의 유효성분으로 유용하게 이용할 수 있다.In addition, the eggshell membrane powder of the present invention increases the moisture on the skin surface, suppresses the transdermal moisture loss, and increases the ceramide of the epidermal layer in an animal model of skin damage induced by UV irradiation. It can be usefully used as an active ingredient in a cosmetic composition for strengthening and protection.
본 발명의 화장료 조성물은 상기 유효성분 이외에 통상적으로 허용되는 성분들을 제한없이 포함할 수 있으며, 예컨대 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 그리고 담체를 포함할 수 있다.The cosmetic composition of the present invention may include, without limitation, commonly acceptable ingredients in addition to the active ingredients, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. have.
본 발명에 따른 화장료 조성물은 용액, 외용연고, 크림, 폼, 영양화장수, 유연화장수, 팩, 유연수, 유액, 메이크업베이스, 에센스, 비누, 액체 세정료, 입욕제, 선 스크린크림, 선오일, 현탁액, 유탁액, 페이스트, 겔, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 패치 및 스프레이로 이루어진 군에서 선택되는 하나 이상의 제형으로 제조할 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition according to the present invention includes solutions, external ointments, creams, foams, nourishing lotions, softening lotions, packs, soft water, emulsions, makeup bases, essences, soaps, liquid detergents, bathing agents, sunscreen creams, sun oils, suspensions, Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, may be prepared in one or more formulations selected from the group consisting of patches and sprays, It is not limited.
또한, 본 발명의 화장료 조성물은 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1 종 이상 추가로 포함할 수 있으며, 통상의 성분으로 예를 들면 유분, 물, 계면활성제, 보습제, 저급 알콜, 증점제, 킬레이트제, 색소, 방부제, 향료 등을 적절히 배합할 수 있으나, 이에 제한되는 것은 아니다. 본 발명의 화장료 조성물에 포함되는 화장품학적으로 허용가능한 담체는 제형에 따라 다양하다.In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, A thickener, a chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.
본 발명의 제형이 연고, 페이스트, 크림 또는 젤인 경우에는, 담체성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 또는 이들의 혼합물이 이용될 수 있다.When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used.
본 발명의 제형이 파우더 또는 스프레이인 경우에는, 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록사이드, 칼슘 실케이트, 폴리아미드 파우더 또는 이들의 혼합물이 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진제를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component. In particular, in the case of a spray, additional chlorophyll propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는, 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되며, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알콜, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일이 이용될 수 있으며, 특히, 목화씨 오일, 땅콩 오일, 옥수수 배종 오일, 올리브 오일, 피마자 오일 및 참깨 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan may be used. have.
본 발명의 제형이 현탁액인 경우에는, 담체 성분으로서 물, 에탄올 [0045] 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알콜, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters, and suspensions such as polyoxyethylene sorbitan esters agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.
본 발명의 제형이 비누인 경우에는 담체 성분으로서 지방산의 알칼리 금속 염, 지방산 헤미에스테르 염, 지방산 단백질 히드롤리제이트, 이세티오네이트, 라놀린 유도체, 지방족 알콜, 식물성 유, 글리세롤, 당 등이 이용될 수 있다.When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. may be used as carrier components. can
본 발명의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시테이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 오일, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcositate, fatty acid amide as carrier components Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.
아울러, 본 발명은 난각막 함유 분말을 포함하는 피부장벽 강화 또는 보호용 피부외용제 조성물을 제공한다.In addition, the present invention provides a composition for external application for skin for strengthening or protecting the skin barrier comprising the eggshell membrane-containing powder.
상기 난각막 분말, 피부장벽 강화 또는 보호에 대해서는 상기에서 설명한 바와 같다.The eggshell membrane powder, strengthening or protecting the skin barrier is the same as described above.
또한, 본 발명의 난각막 분말은 자외선 조사로 유도한 피부손상 동물모델에서 피부표면의 수분을 증가시키고, 경피수분손실을 억제하며, 표피층의 세라마이드를 증가시키는 효과를 나타내므로, 상기 난각막 분말을 피부장벽 강화 및 보호용 피부외용제 조성물의 유효성분으로 유용하게 이용할 수 있다.In addition, the eggshell membrane powder of the present invention increases the moisture on the skin surface, suppresses the transdermal moisture loss, and increases the ceramide of the epidermal layer in an animal model of skin damage induced by UV irradiation. It can be usefully used as an active ingredient in a composition for external application for skin strengthening and protection.
본 발명에 의한 피부외용제 조성물은, 예를 들어, 연고, 로션, 가용화상, 현탁액, 에멀젼, 크림, 젤, 스프레이, 파프제, 경고제, 패치제 또는 물파스 등을 들 수 있지만, 이들에만 한정되지 않고, 당업계에 주지된 어떠한 기제에도 배합될 수 있다. 본 발명에 의한 피부 외용제 조성물은 난각막 분말을 조성물 총 중량에 대하여 0.01 ~ 90 중량%, 보다 구체적으로 0.1 ~ 50 중량%로 함유할 수 있다.The composition for external application for skin according to the present invention may include, but is not limited to, ointments, lotions, solubilizing burns, suspensions, emulsions, creams, gels, sprays, patches, warning agents, patches, or water pastes. It may be formulated with any base known in the art. The composition for external application for skin according to the present invention may contain the eggshell membrane powder in an amount of 0.01 to 90% by weight, more specifically 0.1 to 50% by weight, based on the total weight of the composition.
이하, 본 발명을 실시예 및 실험예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of Examples and Experimental Examples.
단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 한정되는 것은 아니다.However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.
<실시예 1> 난각막 분말의 제조<Example 1> Preparation of eggshell membrane powder
닭의 알 껍질(eggshell)로부터 난각 및 난각막(eggshell membrane)을 분리 및 분쇄하여 난각막 분말을 제조하였다.An eggshell membrane powder was prepared by separating and pulverizing an eggshell and an eggshell membrane from a chicken eggshell.
구체적으로, 닭의 알 껍질과 물을 1 : 3의 중량비로 혼합하고 물로 세척하여 알 껍질에 존재하는 분변 미생물을 제거하였다. 물로 세척한 알 껍질을 햄머밀(Hammer mill)을 이용하여 20분 동안 분쇄하여 알 껍질에서 난각과 난각막을 분리하였다. 상기 분쇄물에 물을 1 : 3의 중량비로 추가하고 교반하여 물 위로 떠오른 분리된 난각막을 획득하였다. 상기 획득한 난각막을 증기 재킷 용기(steam jacketed vessel)에 넣고 80℃에서 45분 동안 열처리하여 1차 살균하였다. 1차 살균한 난각막에 염산 용액을 넣어 pH 4.0으로 유지하면서 80℃에서 45분 동안 열처리하여 2차 살균하였다. 2차 살균한 난각막에서 염산 용액을 제거한 후 건조하였다. 그 다음, 건조한 난각막을 햄머밀을 이용하여 체적최대입자경 400 ㎛, 체적평균입자경 300 ㎛이 되도록 분말화하여 난각막 분말을 획득하였다.Specifically, the chicken eggshell and water were mixed in a weight ratio of 1:3 and washed with water to remove fecal microorganisms present in the eggshell. The eggshell washed with water was ground for 20 minutes using a hammer mill to separate the eggshell and the eggshell membrane from the eggshell. Water was added to the pulverized product in a weight ratio of 1:3 and stirred to obtain a separated eggshell membrane floating on the water. The obtained eggshell membrane was placed in a steam jacketed vessel and heat-treated at 80° C. for 45 minutes to first sterilize. Secondary sterilization was performed by adding a hydrochloric acid solution to the first sterilized egg shell membrane and heat-treating it at 80° C. for 45 minutes while maintaining the pH at 4.0. After removing the hydrochloric acid solution from the secondary sterilized egg shell membrane, it was dried. Then, the dried egg shell membrane was powdered to have a maximum volume particle diameter of 400 μm and a volume average particle diameter of 300 μm using a hammer mill to obtain an egg shell membrane powder.
<실험예 1> 난각막 분말의 피부장벽 강화 효과 분석<Experimental Example 1> Analysis of skin barrier strengthening effect of eggshell membrane powder
<1-1> 자외선 조사에 의한 피부손상 동물모델 제작<1-1> Manufacture of skin damage animal model by UV irradiation
자외선 조사에 의한 피부손상 마우스모델을 제작하고 본 발명의 난각막 분말을 경피투여하였다.A mouse model of skin damage caused by UV irradiation was prepared and the eggshell membrane powder of the present invention was transdermally administered.
구체적으로 난각막 분말의 경구투여를 위해 상기 <실시예 1>에서 제조한 난각막 분말과 부형제로 옥수수 기름(Corn oil)을 혼합하여 30, 60 및 120 mg/kg 농도의 경구용 조성물을 제조하였다. 그 다음,Specifically, for oral administration of the eggshell membrane powder, an oral composition of 30, 60 and 120 mg/kg was prepared by mixing the eggshell membrane powder prepared in <Example 1> and corn oil as an excipient. next,
7주령의 SKH-1 무모쥐(hairless mice, 오리엔트바이오, 한국)를 하기 [표 1]과 같이 G1 내지 G6군으로 나누고, G1 및 G6군을 제외하고 나머지 군에 UVB 조사를 실시하여 자외선 조사에 의한 피부손상을 유도하고, G3 내지 G5군에 상기 제조한 난각막 분말의 경구용 조성물(OD) 각각을 UVB 조사를 실시하는 동안 경구투여하였다. G6군에는 히알루론산(Hyaluronic acid, (주)노바렉스)을 상기에 기재된 방법과 동일한 방법으로 경구용 조성물(HA)로 제조하여 경구투여하였다. G1 및 G2군에는 옥수수 기름을 경구투여하였다. 7-week-old SKH-1 hairless mice (Orient Bio, Korea) were divided into G1 to G6 groups as shown in [Table 1] below, and UVB irradiation was performed on the remaining groups except for G1 and G6 groups to prevent UV irradiation. skin damage was induced, and each of the oral compositions (OD) of the eggshell membrane powder prepared above was orally administered to the G3 to G5 groups during UVB irradiation. In the G6 group, hyaluronic acid (Novarex Co., Ltd.) was prepared as an oral composition (HA) in the same manner as described above and orally administered. Corn oil was orally administered to the G1 and G2 groups.
UVB 조사는 UVB(5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Japan)가 장착된 UV-Irradiation system(LK3500, Korea)을 이용하여 실시하였고, 첫째 주에 1 MED(minimal erythemal dose), 둘째 주에 2 MED, 셋째 주에 3 MED, 넷째 주에서 열째 주까지 4 MED로 실시하였다. 또한, 3회/주(월, 수, 금), 총 12주간 UVB 조사를 실시하여 유도하였다. MED는 예비시험을 통하여 최초의 홍반이 나타나는 자외선 조사량을 측정하여 설정하였다.UVB irradiation was performed using a UV-Irradiation system (LK3500, Korea) equipped with UVB (5 Sankyo Denki G5T5E lamps, Sankyo Denki Co., Japan), 1 MED (minimal erythemal dose) in the first week, and 1 MED (minimal erythemal dose) in the second week. 2 MED, 3 MED in the third week, and 4 MED from the fourth to the tenth week. In addition, UVB irradiation was performed 3 times/week (Mon, Wed, Fri), for a total of 12 weeks to induce. MED was established by measuring the amount of UV irradiation at which the first erythema appeared through a preliminary test.
경구투여는 시험동물의 경배부 피부를 잡아 보정한 후 경구투여용 존데를 이용하여 강제 경구투여 하였고, 1회/일, 7회/주, 12주간(80 일), 투여 당일 오전 중에 투여하였다.Oral administration was performed by compulsory oral administration using a sonde for oral administration after correcting the skin of the cervical region of the test animal, and was administered once/day, 7 times/week, 12 weeks (80 days), in the morning of the day of administration.
<1-2> 일반증상 관찰 및 체중분석<1-2> General symptom observation and weight analysis
본 발명의 난각막 분말의 독성을 확인하기 위하여, 상기 실험예 <1-1>의 마우스모델 각각의 일반증상을 관찰하고 체중을 측정하였다.In order to confirm the toxicity of the eggshell membrane powder of the present invention, general symptoms of each mouse model of Experimental Example <1-1> were observed and body weight was measured.
구체적으로 상기 실험예 <1-1>의 마우스모델의 실험기간 동안 1일 1회 개체별로 일반증상을 관찰하고 투여 당일에는 투여 직후 일반증상을 관찰하였다. 일반증상의 사망여부, 증상의 종류, 발현일 및 증상의 정도를 개체별로 기록하였다.Specifically, during the experimental period of the mouse model of Experimental Example <1-1>, the general symptoms were observed for each individual once a day, and the general symptoms were observed immediately after administration on the day of administration. Death of general symptoms, types of symptoms, onset date, and severity of symptoms were recorded for each individual.
또한, 상기 실험예 <1-1>의 마우스모델에 대하여 입수 시, 분리 시, 투여개시일, 투여 후 주 1회 및 부검일에 체중을 측정하였다.In addition, for the mouse model of Experimental Example <1-1>, body weight was measured at the time of acquisition, separation, administration start date, once a week after administration, and on the day of autopsy.
그 결과, 실험기간 동안 모든 시험군에서 사망동물 및 특이한 일반증상은 관찰되지 않았다.As a result, no dead animals or specific general symptoms were observed in all test groups during the experimental period.
또한, 도 1에 나타낸 바와 같이 모든 시험군에서 시험물질 투여에 따른 체중감소는 관찰되지 않았다.In addition, as shown in FIG. 1 , no weight loss was observed according to the administration of the test substance in all test groups.
상기의 결과를 통해 난각막 분말은 독성이 없음을 알 수 있다.From the above results, it can be seen that the eggshell membrane powder is not toxic.
<1-3> 피부표면 수분함량 및 경표피 수분증발량 분석<1-3> Analysis of skin surface moisture content and transepidermal moisture evaporation
본 발명의 난각막 분말에 의한 피부장벽 강화 효과를 확인하기 위하여, 상기 실험예 <1-1>의 마우스모델 각각의 피부표면 수분함량(Skin moisture) 및 경표피 수분증발량(Transepidermal water loss, TEWL)을 분석하였다.In order to confirm the skin barrier strengthening effect by the eggshell membrane powder of the present invention, the skin moisture content and transepidermal water loss (TEWL) of each mouse model of Experimental Example <1-1> were measured. analyzed.
구체적으로, 상기 실험예 <1-1>의 마우스 모델에 대하여 Corneometer CM825(Courage & Khazaka Electronics, Cologne, Germany)를 이용하여 자외선 조사 전, 자외선 조사 1, 2, 5, 8, 10 및 12주차에 피부표면 수분함량을 측정하였다.Specifically, for the mouse model of Experimental Example <1-1>, before UV irradiation using Corneometer CM825 (Courage & Khazaka Electronics, Cologne, Germany),
또한, 상기 실험예 <1-1>의 마우스 모델에 대하여 Tewameter TM300(Courage & Khazaka Electronics, Cologne, Germany)를 이용하여 자외선 조사 전, 자외선 조사 1, 2, 5, 8, 10 및 12주차에 경표피 수분증발량을 측정하였다.In addition, with respect to the mouse model of Experimental Example <1-1>, before UV irradiation using Tewameter TM300 (Courage & Khazaka Electronics, Cologne, Germany),
그 결과, 도 2에 나타낸 바와 같이, UV 조사량이 증가함에 따라서 정상대조군(G1)과 UV대조군(G2) 간의 피부표면 수분함량의 차이가 증가하였다. 이를 통해 지속적인 자외선 조사에 의해 UV대조군의 피부표면 수분함량이 감소하여 피부장벽이 손상됨을 알 수 있다. 반면, 난각막 분말을 투여한 UV시험군(G3 내지 G5)은 투여 8주차부터 수분함량이 UV대조군(G2)에 비하여 증가하였고, 10주 및 12주차에서는 통계학적으로 유의하게 증가하였다. 특히 12주차에 고함량의 난각막 분말을 투여한 UV시험군(G5)과 양성대조군(G6)의 피부표면 수분함량은 유사하였다.As a result, as shown in FIG. 2, as the amount of UV irradiation increased, the difference in skin surface moisture content between the normal control group (G1) and the UV control group (G2) increased. Through this, it can be seen that the skin barrier is damaged by the reduction of the skin surface moisture content of the UV control group by continuous UV irradiation. On the other hand, in the UV test group (G3 to G5) administered with eggshell membrane powder, the moisture content increased compared to the UV control group (G2) from the 8th week of administration, and increased statistically significantly at the 10th and 12th weeks. In particular, the skin surface moisture content of the UV test group (G5) and the positive control group (G6) administered with a high content of eggshell membrane powder at
또한, 도 3에 나타낸 바와 같이, UV 조사량이 증가함에 따라서 정상대조군(G1)과 UV대조군(G2) 간의 경표피 수분증발량의 차이가 증가하였다. 이를 통해 지속적인 자외선 조사에 의해 유도군의 경표피 수분증발량이 증가하여 피부장벽이 손상됨을 알 수 있다. 반면 난각막 분말을 투여한 UV시험군(G3 내지 G5)은 투여 8주차부터 UV대조군(G2)에 비하여 경표피 수분증발량이 감소하는 경향을 보였고, 12주차에서는 통계학적으로 유의하게 감소하였다. 특히 12주차에 60 mg/kg의 난각막 분말을 투여한 UV 시험군(G4)와 양성대조군(G6)의 피부표면 수분증량이 유사하였고, 120 mg/kg의 난각막 분말을 투여한 UV시험군(G5)은 양성대조군(G6)보다 경표피 수분증발량이 덜 감소하였다.In addition, as shown in FIG. 3, as the amount of UV irradiation increased, the difference in the amount of transepidermal water evaporation between the normal control group (G1) and the UV control group (G2) increased. Through this, it can be seen that the skin barrier is damaged by the increase of transepidermal water evaporation in the induced group by continuous UV irradiation. On the other hand, the UV test group (G3 to G5) administered with eggshell membrane powder showed a tendency to decrease in transepithelial water evaporation compared to the UV control group (G2) from the 8th week of administration, and decreased statistically significantly at the 12th week. In particular, the skin surface moisture increase was similar between the UV test group (G4) and the positive control group (G6) administered with 60 mg/kg of eggshell membrane powder at
상기의 결과를 통해 본 발명의 난각막 분말은 피부표면 수분 증가 및 경피수분손실 억제를 통해 우수한 피부장벽 강화 및 보호 효과를 나타냄을 알 수 있다. 또한, 60 ~ 120 mg/kg의 난각막 분말에서 그 효과가 보다 우수함을 알 수 있다.From the above results, it can be seen that the eggshell membrane powder of the present invention exhibits excellent skin barrier strengthening and protective effects by increasing skin surface moisture and suppressing transdermal moisture loss. In addition, it can be seen that the effect is more excellent in the egg shell membrane powder of 60 ~ 120 mg / kg.
<1-3> 피부조직 내 세라마이드 함량 분석<1-3> Analysis of ceramide content in skin tissue
세라마이드(Ceramide)는 피부 표피층 중 각질층을 구성하는 지질로 층상구조를 유지시켜 피부의 수분과 피부장벽을 유지하는 역할을 한다. 이에, 본 발명의 난각막 분말에 의한 피부장벽 강화 효과를 확인하기 위하여, 상기 실험예 <1-1>의 마우스모델 각각의 피부조직 내 세라마이드 함량을 분석하였다.Ceramide is a lipid constituting the stratum corneum among the epidermal layers of the skin. Therefore, in order to confirm the skin barrier strengthening effect of the eggshell membrane powder of the present invention, the ceramide content in the skin tissue of each mouse model of Experimental Example <1-1> was analyzed.
구체적으로, 상기 실험예 <1-1>의 마우스 모델에 대하여 UV 조사 종료 후 cervical dislocation의 방법으로 희생시키고 피부조직을 절취하여 진피를 아랫방향으로 넣은 후 HBSS(Hank’s Balanced Salt Solution, RNBH1207, Sigma)와 Dispase solution (grade II, 2.4 unit/mL, 37045800, Roche)을 1 : 1 비율로 혼합한 용액을 첨가하여 4℃에서 18시간 동안 반응시켜 표피를 분리하였다. 그 다음, folch 용액 400 ㎕(Chlroform : methanol = 2 : 1, v/v)을 첨가하여 3,000 rpm, 5 분 동안 원심분리하고 하층액을 LC/MS/MS를 이용하여 [표 2]의 조건으로 분석을 실시하였다.Specifically, for the mouse model of Experimental Example <1-1>, after UV irradiation was completed, the cervical dislocation method was sacrificed, the skin tissue was cut and the dermis was placed downward, and then HBSS (Hank's Balanced Salt Solution, RNBH1207, Sigma) and Dispase solution (grade II, 2.4 unit/mL, 37045800, Roche) in a 1:1 ratio was added and reacted at 4°C for 18 hours to separate the epidermis. Then, 400 μl of folch solution (Chlroform : methanol = 2 : 1, v/v) was added, centrifuged at 3,000 rpm for 5 minutes, and the supernatant was subjected to the conditions of [Table 2] using LC/MS/MS. Analysis was performed.
temp.auto sampler
temp.
Detector(MS/MS)
Detector (MS/MS)
Nebulizing gas : N2Positive ion mode, MRM mode
Nebulizing gas : N2
energy(V)Collision
energy(V)
그 결과, 도 4에 나타내 바와 같이, 자외선 조사 종료 후 정상대조군(G1)과 UV대조군(G2) 간의 세라마이드의 차이는 보이지 않았다. 이는 세라마이드가 자외선 조사에 따라 피부방어 기작에 의하여 일정기간 동안 표피층에서 증가를 보이는 현상 때문으로 볼 수 있다(Kim H et al., J Med Food 2009; 12(4): 746-54). 반면, 난각막 분말을 투여한 UV시험군(G3 내지 G5)에서는 UV대조군(G2)에 비하여 세라마이드 농도가 통계학적으로 유의하게 증가하였다. 특히 60 mg/kg 및 120 mg/kg의 난각막 분말을 투여한 UV 시험군(G4 및 G5)는 양성대조군(G6)보다 세라마이드 함량이 더 증가하였고, 60 mg/kg의 난각막 분말을 투여한 UV 시험군(G4)에서 그 효과가 더 우수하였다.As a result, as shown in FIG. 4 , there was no difference in ceramide between the normal control group (G1) and the UV control group (G2) after the UV irradiation was finished. This may be attributed to a phenomenon in which ceramides increase in the epidermis for a certain period of time by the skin defense mechanism according to UV irradiation (Kim H et al., J Med Food 2009; 12(4): 746-54). On the other hand, the ceramide concentration was statistically significantly increased in the UV test group (G3 to G5) administered with eggshell membrane powder compared to the UV control group (G2). In particular, the UV test group (G4 and G5) administered with 60 mg/kg and 120 mg/kg of eggshell membrane powder had a higher ceramide content than the positive control group (G6), and UV test with 60 mg/kg of eggshell membrane powder The effect was better in the group (G4).
상기의 결과를 통해 본 발명의 난각막 분말은 표피층의 세라마이드를 증가시켜 우수한 피부장벽 강화 및 보호 효과를 나타냄을 알 수 있다. 또한, 60 ~ 120 mg/kg의 난각막 분말에서 그 효과가 보다 우수함을 알 수 있다.From the above results, it can be seen that the eggshell membrane powder of the present invention exhibits excellent skin barrier strengthening and protective effects by increasing ceramide in the epidermal layer. In addition, it can be seen that the effect is more excellent in the egg shell membrane powder of 60 ~ 120 mg / kg.
Claims (15)
A health functional food composition for strengthening or protecting skin barrier, comprising eggshell membrane powder.
1) 조류의 알 껍질과 물을 1 : 0.5 내지 10의 중량비로 혼합하고 물로 세척한 후 분쇄하여 분쇄물을 획득하는 단계;
2) 획득한 분쇄물에 물을 첨가하고 교반하여 난각막을 획득하는 단계;
3) 획득한 난각막을 증기로 열처리하여 1차 살균하는 단계;
4) 1차 살균한 난각막에 염산 용액을 넣고 pH 3.0 내지 4.5에서 가열하여 2차 살균하는 단계; 및
5) 2차 살균한 난각막을 원심분리하여 염산 용액을 제거한 후 건조하여 난각막 건조물을 획득하는 단계; 및
6) 획득한 난각막 건조물을 분말화하여 난각막 분말을 획득하는 단계.
The health functional food composition for strengthening or protecting the skin barrier according to claim 1, wherein the eggshell membrane powder is prepared through the following steps:
1) mixing the egg shell of algae with water in a weight ratio of 1: 0.5 to 10, washing with water, and pulverizing to obtain a pulverized product;
2) adding water to the obtained pulverized product and stirring to obtain an eggshell membrane;
3) primary sterilization by heat-treating the obtained eggshell membrane with steam;
4) adding a hydrochloric acid solution to the first sterilized eggshell membrane and heating at pH 3.0 to 4.5 for secondary sterilization; and
5) centrifuging the second sterilized egg shell membrane to remove the hydrochloric acid solution and drying it to obtain a dried egg shell membrane; and
6) pulverizing the obtained eggshell membrane powder to obtain eggshell membrane powder.
[Claim 3] The health functional food composition for strengthening or protecting the skin barrier according to claim 2, wherein in step 1), the egg shell of the algae and water are mixed in a weight ratio of 1:1 to 5.
[Claim 3] The health functional food composition for strengthening or protecting the skin barrier according to claim 2, wherein the egg shells of algae are pulverized for 10 to 30 minutes in step 1).
[Claim 3] The health functional food composition for strengthening or protecting the skin barrier according to claim 2, wherein in step 3), the egg shell membrane is heat-treated with steam at 65 to 95° C. for 30 to 60 minutes.
The health functional food composition for strengthening or protecting the skin barrier according to claim 2, wherein the egg shell membrane sterilized in step 4) is heated at 65 to 95° C. for 20 to 40 minutes.
The health functional food composition for strengthening or protecting the skin barrier according to claim 2, wherein the eggshell membrane powder in step 6) has a volume average particle diameter of 100 to 400 μm.
The health functional food composition for strengthening or protecting the skin barrier according to claim 1, wherein the eggshell membrane powder contains 3 to 12 mg/kg in an effective amount per day.
The health functional food composition for strengthening or protecting the skin barrier according to claim 1, wherein the skin barrier strengthening or protection is strengthening or protecting the skin barrier against ultraviolet rays or fine dust.
The health functional food composition for strengthening or protecting the skin barrier according to claim 9, wherein the ultraviolet rays are ultraviolet B (ultraviolet-B, UVB).
The health functional food composition for strengthening or protecting the skin barrier according to claim 1, wherein the composition is prepared in one or more formulations selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage.
A food composition for strengthening or protecting the skin barrier, comprising a powder containing eggshell membrane.
A cosmetic composition for strengthening or protecting the skin barrier, comprising a powder containing eggshell membrane.
14. The method of claim 13, wherein the cosmetic composition is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil. , suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, one or more formulations selected from the group consisting of patches and sprays, A cosmetic composition for strengthening or protecting the skin barrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200036079A KR20210119680A (en) | 2020-03-25 | 2020-03-25 | Composition for enhancing skin barrier comprising eggshell membrane powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200036079A KR20210119680A (en) | 2020-03-25 | 2020-03-25 | Composition for enhancing skin barrier comprising eggshell membrane powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20210119680A true KR20210119680A (en) | 2021-10-06 |
Family
ID=78077191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020200036079A KR20210119680A (en) | 2020-03-25 | 2020-03-25 | Composition for enhancing skin barrier comprising eggshell membrane powder |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20210119680A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230098055A (en) | 2021-12-22 | 2023-07-03 | 주식회사 노바웰스 | Composition for improving skin condition comprising eggshell membrane micropowder |
-
2020
- 2020-03-25 KR KR1020200036079A patent/KR20210119680A/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230098055A (en) | 2021-12-22 | 2023-07-03 | 주식회사 노바웰스 | Composition for improving skin condition comprising eggshell membrane micropowder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3914244B2 (en) | Abnormal protein removal composition | |
| JP3966922B2 (en) | Fibroblast growth promoter | |
| JP4205188B2 (en) | Maillard reaction inhibitor | |
| JP3966924B2 (en) | Fibroblast growth promoter containing plant extract | |
| KR20150050310A (en) | Composition for improving skin conditions comprising artemisiae annuae herba, neem leaf, centella asiatica and green tea extract or fraction thereof and method for improving skin conditions using the same | |
| KR101787074B1 (en) | Cosmetic compositions comprising mixed extracts of natural products for skin-whitening and improving skin-wrinkle | |
| KR101360760B1 (en) | Fermented ear shell extract and its cosmetic usage | |
| KR101437997B1 (en) | Fermented caviar extract and its cosmetic usage | |
| KR101996732B1 (en) | Cosmetic composition comprising concentrate of Omegiju fermented using magma seawater for improving wrinkles, elasticity or moisturizing the skin | |
| KR20210119680A (en) | Composition for enhancing skin barrier comprising eggshell membrane powder | |
| KR102139659B1 (en) | Composition for improving the skin | |
| KR101914628B1 (en) | Composition for skin anti-wrinkle effect comprising oil from plant as effective component | |
| KR101855989B1 (en) | Method for producing buckwheat seed extract with increased effective component using water adding solubilizer as extraction solvent and buckwheat seed extract produced by the same | |
| KR101914441B1 (en) | Cosmetic compositions for improving skin moisturizing comprising fucosterol | |
| KR20190063926A (en) | Composition for preventing or alleviating intrinsic aging comprising paeoniflorin or albiflorin | |
| JP4169814B2 (en) | A topical skin preparation containing agave or sisal extract | |
| KR102212343B1 (en) | Composition for skin anti-aging or moisturization comprising extract of marian plum | |
| KR101964837B1 (en) | Composition for improving skin wrinkle comprising Cynomorium songaricum extract or its fraction as effective component | |
| KR20160054672A (en) | Composition for promoting synthesis of hyaluronic acid comprising Hordeum vulgare extracts and the use thereof | |
| KR20200048225A (en) | The obtaining method of compound | |
| KR102355138B1 (en) | Composition for whitening or anti-wrinkle of the skin comprising fermentated ziziphus jujuba seed as effective component | |
| KR20230098055A (en) | Composition for improving skin condition comprising eggshell membrane micropowder | |
| JP4707734B2 (en) | Skin preparation containing sisal extract | |
| KR102326086B1 (en) | Composition for Improving Skin Conditions Having Skin Whitening, Moisturizing, Anti-Wrinkle and Skin Barrier Property Comprising Complex Extracts of Broussonetia kazinoki as Active Ingredient | |
| KR102299417B1 (en) | Composition for inducing alteration of surrounding environment of a aged cell |