JP4707734B2 - Skin preparation containing sisal extract - Google Patents

Description

  The present invention relates to a lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor containing a novel and safe sisal extract, and various skin external preparations and baths in the pharmaceutical / quasi-drug or cosmetic field. The present invention relates to the application to drugs or foods and drinks.

  It can be used in various internal and external preparations (including preparations used for animals), specifically, ampoules, capsules, pills, tablets, powders, granules 1) Pharmaceuticals, 2) Quasi-drugs, 3) Foods, 4) Topical or systemic skin cosmetics, 5) Medicinal products applied to scalp and hair Or / and cosmetic preparations (for example, shampoos, rinses, treatments, permanent liquids, hair styling agents, hair styling agents, hair growth / hair restoration agents, etc.), 6) bath preparations used in bath water, 7 ) In addition, liquid odors and deodorants, hygiene products, sanitary cotton, wet tissue, etc. are raised.

  Furthermore, as foods and drinks, oral compositions (gum, candy, etc.), processed fishery products such as kamaboko and chikuwa, livestock products such as sausages and ham, Western confectionery, Japanese confectionery, raw noodles, Chinese noodles, boiled noodles, buckwheat, etc. Seasonings such as noodles, sauces, soy sauce, sauce, sugar, honey, powdered candy, syrup, etc., spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, red vegetables, sprinkles, or Processed vegetables and fruits such as canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soup, fruit juice, vegetable juice, whey drink, soft drinks, alcoholic beverages, and other health foods It can be used for general foods and beverages.

  Body fat in the subcutaneous tissue plays an important role in protecting health, especially energy storage, protein saving, body temperature regulation, expression and maintenance of menstruation, etc. When body fat accumulates excessively Causes various illnesses, for example, hyperlipidemia, and as a result of excessive body fat, a lot of fat deposits on the walls of the arteries, reducing the elasticity and hardening of the arteries. Such a condition is called arteriosclerosis and is said to lead to angina pectoris and myocardial infarction, and also causes hypertension, causing stroke and heart attack. In addition, diabetes and bone joint damage due to weight gain occur.

  Therefore, reducing fat is very important for health, and in order to reduce fat, lipase enzyme is used and triacylglycerol is decomposed into fatty acid and glycerin, for example, in the base Lipase itself encapsulated in cosmetics (Japanese Patent Laid-Open No. 62-220598), or Yokuinin extract used as a sebum secretion promoter (Japanese Patent Laid-Open No. 60-174723), orchidaceae: Shiran extract is effective for oily skin acne (Japanese Patent Laid-Open No. 57-102809), and as a sebum suppressor, there are cyproterone acetate by oral administration, but the effect is not enough, such as rash There is a problem in terms of side effects that cause hypersensitivity and nausea, and no safe lipase activity promoter has been found when administered orally or transdermally.

  It is possible to reduce body fat through dietary restrictions, but the balance between daily energy intake and scent factor will be lost, and conversely, body weight will be dramatically reduced, and then weight reversal will increase.・ There is a possibility of harming the physical condition, and when it is used as a skin external preparation or bath preparation, moisture necessary for the skin may be removed, causing rustiness and rough skin.

  In view of these circumstances, the present inventors have developed various plants that are useful as lipase activity promoters, moisturizers, whitening agents, or contact dermatitis inhibitors, and as a result, promoted lipase activity in sisal extracts. It has been confirmed that it has an action, a moisturizing action, a melanin production inhibitory action, and a contact dermatitis inhibitory action. Therefore, it can be effectively used as a lipase activity promoter, moisturizer, whitening agent, contact dermatitis inhibitor, and skin. Or suppresses fat on the scalp, prevents skin diseases of oily skin (for example, acne, seborrheic eczema, seborrheic alopecia, dandruff, etc.), inflammatory skin diseases such as rough hands and kayumi, and prevention of dry skin, Effective for treatment. In addition, it promotes fat decomposition, reduces excess fat in the body, moisturizes the skin, moisturizes the skin, and can be used as a whitening agent to prevent sunburn, etc. By providing a product, the present invention has been completed.

  In the present invention, an extract of sisal has a lipase activity promoting action, a moisturizing action, a melanin production inhibiting action, and a contact dermatitis inhibiting action, and is safe even if used internally or externally for humans or animals. . Therefore, it can be used effectively as a lipase activity promoter, moisturizer, whitening agent, contact dermatitis inhibitor or anti-inflammatory agent, and further to all forms of preparations (pharmaceuticals, quasi-drugs, cosmetics, foods). Application is also possible. It also suppresses fat on the skin or scalp, skin diseases of oily skin (for example, acne, seborrheic eczema, seborrheic alopecia, dandruff, etc.), inflammatory skin diseases such as rough hands, Kayumi, and dry skin It is effective for prevention and treatment. It can also be used as a whitening agent that promotes fat decomposition, reduces excess fat in the body, moisturizes the skin and prevents sunburn and the like.

  The plant “sisal” used in the present invention is obtained from whole plants, fruits, flowers, leaves, stems, bark, roots, etc. of Agave sisalana Perr. (A. rigida Mill.). In addition, A. cantala Roxb., Heneken (A. fourcroydes Lem.), And the like can also be used.

  The sisal extract used in the present invention is obtained by extracting various parts (whole plant, fruit, flower, leaf, stem, bark, root, etc.) of each plant as they are or after pulverization. Or / and extracted as is or after pulverization with a solvent.

  Examples of the extraction solvent include water, alcohols (for example, lower alcohols such as methanol, absolute ethanol, and ethanol, or polyhydric alcohols such as propylene glycol and 1,3-butylene glycol), ketones such as acetone, diethyl ether, and dioxane. , Esters such as acetonitrile and ethyl acetate, and organic solvents such as xylene, benzene and chloroform can be used alone or in any combination of two or more of them, and each solvent extract can be used in combination. Can be used even in

  Sisal extract can be dried, concentrated or diluted depending on the lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor, skin external preparation or bath preparation, food and beverage dosage form / form. Just adjust it.

  The production method is not particularly limited, but is usually in the range of the boiling point of the solvent at room temperature to normal pressure. After extraction, filtration or ion exchange resin is used for adsorption, decolorization, and purification. A solution, paste, gel, or powder may be used. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as it does not affect its efficacy. An activated carbon column or the like may be used, and usual means generally applied depending on the extracted substance may be arbitrarily selected.

  The extract of the sisal of the present invention can be used as it is as a lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor, and can also be added to a skin external preparation or bath preparation, food and drink, Is not particularly defined, but may vary slightly depending on the lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor, skin external preparation or bath preparation, food and drink type, quality, and expected action. In general, it is 0.05% by weight or more (hereinafter, expressed by weight%), preferably 0.1 to 60%. If the blending amount is less than 0.05%, the effect cannot be expected sufficiently.

  The lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor, external preparation for skin or bath preparation, food and drink of the present invention do not impair the effects of the present invention, if necessary, in addition to the above essential components. Within the range, it can be produced by using components and additives used in preparations such as pharmaceuticals, quasi-drugs, cosmetics and foods and drinks.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef fat, beef tallow fatty acid, cucumber nut oil, Safflower oil, shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cacao butter, palm oil, palm kernel oil, molasses , Palm oil, beef tallow, lard, or hydrogenated products of these oils (hardened oil, etc.).

(2) Wax beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, etc.

(3) Mineral oils Liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, squalene, squalane, pristane, etc.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid, 2- Synthetic fatty acids such as ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols: natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, etc.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate, etc.

(9) gum, saccharides and water-soluble polymer compounds gum arabic, benzoin rubber, danmar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or esters thereof, Trehalose or its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan with addition of alkylene (C2-C4) oxide such as ethylene oxide, low molecular chitin Or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methylcellulose, carboxy Polymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide, polypropylene oxide, etc. Alkylene oxide or its crosslinked polymer, carboxyvinyl polymer, polyethyleneimine, etc.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), amphoteric Surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant ( Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants ( Natural surfactants, protein hydrolyzate derivatives, polymer surfactants, surfactants containing titanium and silicon, fluorocarbons System surfactant), and the like.

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: ascorbic acid or its derivatives, vitamin D group: ergo Calciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxolol, vitamin E group: tocopherol or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2), menadione ( Vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine , Arginine, ornithine, histidine, etc., and their amino acid derivatives such as sulfate, phosphate, nitrate, citrate, or pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, fermentation, purification) , Pressing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc.) You can use it.

  The solvent used for extraction may be selected in consideration of the intended purpose and type of the product to be provided, or the processing performed later. Usually, water, a water-soluble organic solvent (for example, ethanol, propylene glycol) , 1,3-butylene glycol, and the like) are preferably used. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and can be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

  In addition, when applying additives derived from plant or animal-based raw materials to systemic or topical external preparations and cosmetics, not only the protection of skin and hair, but also moisturization, improvement of touch / feel, provision of flexibility, stimulation Relaxation, stress relief by fragrance, cell activation (cell aging prevention), suppression of inflammation, improvement of skin / hair quality, prevention of rough skin or / and improvement thereof, hair growth, hair growth, prevention of hair loss, imparting luster, cleanliness In addition to cosmetic effects such as effects, relief of fatigue, blood flow promotion, and warm bath effects, effects such as flavoring, deodorizing, thickening, preserving, and buffering can also be expected.

  In edible products, nutritional supplementation, fatigue recovery, tonicity, cell activation (cell aging prevention) and other healthy and cosmetic effects, as well as improved taste, color and aroma, gloss, stabilization, thickening, It can be used for antiseptic purposes. In addition to this, various cosmetic and pharmaceutical effects of each of the known raw materials are expected, and by combining these, the intended effect can be promoted or multifunctional. It is also possible to make the product expected to be effective.

  Specific plants (herbal medicines) used as raw materials include, for example, almond (amynna), ai (aiba), aokazura (Keifu Fuji), aotsu-rafuji (wooden moth), acacia, red-currant fruit, red grape, red bud , Akane (Rhizome Root), Akaiyasio Jiou (Ghihuang), Agi (Atsu), Akinire (Crustacea), Akebi (Kitsu), Asa (Asako Jin), Maruba Asagao or Asagao (Toshigyu), Ashitaba ( Tomorrow), azuki bean (red bean), acerola, asenyaku (Asenyaku), anise, abemaki fruit, avocado, flax, achacha (Amacha), achacharu, amadokoro (tamatake), amigasayuri (shellfish mother), altea, arunika, aloe (蘆薈), aloe vera, angelica, apricot honanzu (Anzujin), Ansokkou (benzoic incense), igakozorina (Gallhead), Japanese knotweed (tiger cane root), strawberry, fig (no flower fruit) or its leaves, ichibi ( Citron), Ichiyakusou, Ginkgo (Ginkgo leaves, Ginkgo), Itohimehagi (distant), Carob, rice seed or seed coat, Inondo seed, Ibukizasou, nettle, Ylang-ylang, Fennel (Tsukuka), Ukiyagara (Three ridges) Fruits, Japanese cypress, turmeric (Usukin), Usubasaishin Keirinsaishin (spicy), Usbeniaoi, Usubenitachiaoi, Nezubusa (Summer hay), Udo or Shishido (Cultivation of activity, Independence of activity, Tang Dynasty), Ume (Plum) Flesh, Vulture, Uwaurushi, Satsumikan (Chest), Estragon, Ezoukogi (Kagogoka), Enoki, Ebisugusa (Keiko), Elderberry fruit, Eremi, Enju (Shihana, Satsuma rice), Ogi Kibanaougi (Yellow) ), Ouren (Huang Ren), Ookarisuri (Karonin), Otsuka Rafuji (Preventive Self), Psyllium (car front child, car front) ), Toucan's beetle (榛子), Ovana okera okera (white birch), Obaya chabushi fruit, Oha hawthorn hawthorn (Yamakoko), Barley (barley), Oguruma (turnover), Okra fruit, Panax ginseng, Tochiban ginseng (carrot), Hypericum perforatum Scarlet moth, Hypericum perforatum (cutting younger brother), Odorikosou (continuous), Onamomi (Circle ear), Onigurumi, Onino Yagara (Tempa), Onyuri Sasayuri Hakatayuri (lily), Ononis, Halibut Soy sauce), Dutch strawberry, Dutch mustard (watercress), Dutch jellies, Dutch honey bees, olives, oregano, orange fruit or fruit peel, kaikaijiou (ripening yellow), cacao seeds, oysters or their leaves (bamboo shoots), scallops (fishing wisteria)鈎), cassia, casino berries (fruit), gadgets (莪 朮), kashiwa (bamboo tree, coconut leaves), cascarilla, Nigusa (Kinshato), valerian (Yakusa-root), birch or weeping birch (white birch), pumpkin, kapok seed, kahoku salamander (navy blue), gama (yellow), chamomile, roman chamomile, camilla deer (weed), crow lily (king)瓜), crow birch (half-summer), oats, guarana seeds, karaya, karin (wood persimmon), kawayanagi, kawaratake, kawara mugwort, kansui (kansai), licorice (licorice), candelilla, kanto, kanna, raspberry , Kiwi fruit, Kikarasuuri (Gurone), Oyster (Kikkyo, Kikyo Root), Chrysanthemum (Chrysanthemum flower), Kisage (Kashimi), Gishigishi (Yotei), Kijitsu (Kakimi), Kizuta, Kidachi Aloe, Kidachihakka, Kinanoki, Yellowfin tuna, Gymnema sylvestre, cabbage, cabbage immature fruit, cucumber, Kiraja saponaria, kiraya, kumquat fruit, betel, kimme Hiki (Sengakurasaku), guava fruit, guayule, quercus infectria (garlic), kukui nuts, wolfberry (bamboo shoots, coconut leaves, cocoon leaves, ground bark), kusasugikazura (Tenmon Fuyu), kudu (kudzu), camphor tree, goose Berry fruit, gardenia (Yamako), Kunugi (Kusou), Kumazasa, Kumatsutsura (horse whip grass), Clara (bitter), cranberry fruit, chestnut or its astringent skin, Curkurigo latifolia fruit, grapefruit fruit, buckthorn, blackhead ), Cloves (clove, clove), keigai (butterfly, cocoon), celosia (chicken flower, chicken clover), gecko (bay laurel), gentiana, gennoshouko (old lady), koshinbara (moon flower) (Melissa), Kozo fruit, Kocha (tea), Kohone (riverbone), Kohon (Tsubakimoto, Karamotomoto), Kouliang, Cilantro fruit, Koganebana ( Gon), Cowberry (Etsutachibana), Coconut fruit, Goshuyu (Kurean), Goshostrawberry (Golden bonsai), Pepper (Pepper), Copaiba balsam, Coffee beans, Kobushi, Tamshiba, Mokuren (Hot pepper), Burdock (Beef bowl, Gyudon) ), Coconut lees, sesame seeds, sesame seeds (genus ginseng), wheat (wheat), rice and rice bran (red cocoon, white birch) and rice oil, cola acminata seeds, cola vera seeds, colombia fruit, colombo, condurango, Kombu, Konjac, Comfrey (Sardine), Saxima button eel (wei Sen-sen), Sakura leaves / flowers / fruits / bark (cherry bark), cherries, pomegranate, Sasa, Sasayuri (lily), Sasanka, Sasodamodaka , Sweet potato, sugar cane, sugar beet, sunflower jujube (acid soy sauce), saffron (banban flower, west red flower), pomelo fruit, bonito, salmon berry fruit, arabication Salvia (Sage), Sagaki Kyo (Yamabana), Hawthorn (Yamazako), Sanshichininjin (Thirty-seven Ginseng), Sanshuyu (Yamatake), Sansho (Yamatake), Sandskon (Yamazune), Shea (Kalite), Cyanoki fruit, shiitake mushroom, shion (purple spider), jikitarisu, shikunshi (messenger), perilla, ajizo, chillimenzo, catamendizo (purple leaf, shisoko), rosewood, linden tree, syringen (ream), pelicans (chrysanthemum) , Shimotsuke, Potato, Peonies (Shakuyaku), Shajin (Sanjin), Janohige (Wheat Gate Winter), Juzudama, Palm Fruit, Ginger (Ginger), Shobu (Koji, Sone), Shozuk Fruit, Shirakashi Seed, Shirogocho Seed , Shirobanairisu, Shirobanatsuta flower, Shirominanten (Nan Tenji), Cinconacill bra, Sinnamon, Watermelon (Sairan), Honeysuckle (Gin silver flower, Shinobu winter), Sui (Acid), Swimberry fruit, Strawberry fruit, Horsetail (Question), Stevia, Plum fruit, Scots pine cone, Japanese larch, Japanese walnut, Japanese walnut, Japanese hawthorn, Japanese dandelion, Japanese horse chestnut (Maroni), Japanese pear Fruit, Azalea, Elderberry, Apricot, Achillea millefolium (milfoil), Rose, Alaskania, Iris mistletoe, Acacia serrata, Acacia, Azalea, Azalea, Acacia Celery, nematode (river cucumber), sendan, senburi (medicated), senna fruit or leaf, ginseng (large persimmon), soka (grass), buckwheat seed, rice potato, daiou (daihuang), radish, soybean, daidai (orange) Leather ), Takatodai (Daikan), Takara-bi (Dark spine), dark sweet cherry fruit, hollyhock, red-headed pearl millet (thyme, perilla), tachibana (tachibana bark), tachiyanagi, tamarind seeds, onion, red-crowned ginger, taranoki or its Root bark, Tanjin (dansang), Dandelion (Sanko) or Shirobana dandelion, Mouko dandelion, Danmaru, Cherry fruit, Chigaya or its root (sone root), Chixetsujinjin (Bamboo ginseng), Chicory, Datura (Dai yellow), Korean ginger (carp), ginseng (carrot), ginseng (carp leaf), choreimaitake (carp), camellia, camellia, camellia, clover (lacquered weed), camellia (duck weed) , Tsuruzuki (red bean), Tsurudokudami (what neck rice cake), Tsuruna (Apricot), Tsuru carrot (Yotsuban), Tsuwabuki, Deiko, Teuchigu Mi, Duberry fruit, Tengusa, Tendai-yaku (glaze), Toga (winter coconut), Pepper (banban), Touki (Toshiki), Toki-Senka (Marigold), Corn or corn hair (Southern cocoon), Tonenmumochi ( Sadako), Torindo (Ryuboku), Dokudami (10 medicines), Tokon (Nuke root), Tochibaninjin (Bamboo ginseng), Tochu (Tsunachu, Tochunakaba), Tomato, Tragacanth, Triacanthus seeds, Tormentilla, Doronoki, Torooaoi, Nai Zeriaberry fruit, Chinese yam (Yakuyaku), Nagai Kada (Butcher Bloom), Naginata Koju, Nazuna, Rapeseed, Natsumi, Natsume (Otsuchi), Naniwaibara (Golden Cherry), Narco Lily (Yellow), Nankin Bean (peanut), Nanten (Nami Tenmi), Nigaki (Nagi), Nigamugigi (Anniversary), Nikuzuku, Nikkei (Kizuna), Nira (Zushi), Elderberry (bone graft) Berries, flowers or stems, garlic (large coral), nurde (penta), leek, white thistle (large coral), Neubara (fruit), yarrow, scallops, wild rose, peach, palm, pineapple fruit, hibiscus , Fulinbussouge, Roselle), Hakatayuri (lily), Hakamaurabosi (bone fracture), hakusen (white crust), hakuruberry fruit, chickweed (breeding), hazelnut (lion), ashridokoro (roth root), basil, lotus (lotus) , Renko), Parsley (Netherlands jelly), Hadakagi, Batata, Hachiku Madake (Bamboo bamboo), Patchouli, Hakka (Light load, Thin load leaf), Barley (Yokuinin), Hanasuge (my mother), Banana, Hanahaka, Vanilla bins, Papaya , Hakokusa (Circium weed), hub grass, paprika, cicada mitsuba cicada (bendid lion), hamasuge (Katsukiko), Amaranthus (spotted lion), hermanus (Maikai) ), Hamaboufu (beach wind), Hamelis, Rose (rose), Parietalia, Harnile (crustacea, cinnabar skin, cocoon leaf), Pannoki, Hyogi (glow-dried), Hikageturinjin (participant), Pecan nut, Higanbana (Ishibuchi, Bujushahua), Hikikoshi (long life grass), Hishi (Hishimi), Pistachio, Beet, Hitotsuba (Ishizuchi), Hinata Taikozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Pepper, Himegama (koi), Japanese matsutake, pimenta fruit, peony, jade fruit, belude oyster, white-bellied oyster (bald eagle), loquat (bamboo shoot, cocoon leaf), betel row (large clothes, coconut), sweet pod (southern wicker), Japanese pheasant, dandelion winter flower, Subsidized winter leaves), Fujibakama (Orchid), Fuji bean (Tonzu), grape fruits or pericarps / leaves, beech, Fuyumushinatsusatake (Cycium summer grass), Brazilian licorice, black currant fruit, black Berry, plum fruit, flucellaria, blueberry (Prunus serrata), prunes, blond psyllium, bundo (mung bean), loofah, safflower (safflower), belladonna, berry fruit, boysenberry fruit, bowfowl (windproof), spinach, physalis (Toro) Root), Honoki (Seki-Pak, Japanese Kotsu-Park), Bokeh (Kiso), Hosobana Okera (Kaki), Hosobabarengiku, Bodaiju (Koki), Button (peony, peony skin), Hop, Jojoba, White berry fruit, Honshimeji, Mizurutennansho (Tennansei), Maou (mao), macadamia nuts, macri (marine grass), mugwa (mulberry white skin, mulberry leaf), matata (wood tengu), matsukasa, pine needles, matsuhodo (茯苓), mayoram ( Hana-hakka), malvanojadine (bitter), mulberry fruit, quince, maronier, mango, manshu gurumi, mandarin fruit, ma Nentake (Ganoderma lucidum), Citrus genus fruit (fruit), Mishimashiko (Shibako), Mizokakushi (half-sided lotus), Misohagi (Senka green), Mitsugashi, Mitsuba, Mitsubahamago (Vulgaris), Green mint, Mimosa, Myoga, Miracle Fruit, Myrrh, Milobaran, Mugge (Mushroom), Mukunoki, Mukuroji, Murasaki (purple root), Murasaki Maize, Mejijiki (Masameki), Meubougi, Melaroika, Melissa, Melilot, Melon Fruit, Moukomugi, Mosoukoku Incense), Japanese maple leaf, Peach leaf (peach leaf), Fruit, Seed (Momojin), Palm, Morello cherry fruit, Morohaya (Hemp), Yakawamuraimo, Yakuchi (Michichi), Cornflower (cornflower), Cornflower mint , Yashabushi (Yagar), Yachiyanagi, Yatsude (octagonal gold plate), Mistletoe (willow infestation), Willow tree leaf, Yabgarashi, Ya Koji (purple gold beef), pokeweed (commercial land), yamahanoki (yamabe), yamamomo (bamboo ume skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca flavifolia, yuzu fruit, lily, sagebrush, mugwort , Lime fruit, rye, lacanca fruit, raspberry leaf / fruit, lavender, longan (long-eye), ryokcha (green tea), apple fruit, gentian, rubus suabisimus (cool), lettuce, red currant fruit, lemon fruit, lemon Glass, forsythia, forsythia, wax palm, loganberry fruit, logwood, rosemary (mannenrou), rosehip (nobara), wakame, wasabi, walmocow (ground).

  Seaweeds include seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Usbaaonori, Susiaoonori, Hiraaoori, Boaonori, Hosoedaonori], Seaweed [Brown algae: , Hirome, Aowakame, Macrocystis pirifera, Macrocystis integrofolia, Neocitus Ruetkeana, Hijiki, Hibamata], Seaweed [Red algae: Hijirimen, Maca (Tengusa), Hiluxa, Onyxa, Ochusa, Kataobagusa, Yatabegusa, Yuikiri, Japanese pterosa, Tokakanori, Togekininsai, Amakusarinin, Kirinsai, Bakishinrinin, Tsunomata, Oobatsunomata, Tochaka (Yahazunomata), Ezotsunomata, Typical examples include Togettsu no Mata, Hirakotoji, Kotoji No Mata, Sugi Nori, Shikinori, Kainori, Ibotsunamata, Yareus Banori, Kagius Banori, Susius Banori, and Heius Banori].

  In addition, other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunariella: Dunaliella, Chlorococcus: Chlorococcus, Kuwanomimo: Kuwanomimo, Volbox: Ohigemawari, Volbox, Palmera, Yotsumemo, Hizaori, Aoumikuro, Tsurumimidro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa: Ooshiogusa, Asamirishigusa, Kawasiogusa, Marimo, Maragolia Magama genus: Magamata, Iwagata: Fusaikawa, Surichogita, Heraiwata, Kurokita, Hanemo, Genus Mill: Mill, Chromel, Sakibutimil, Nagamil, Hira Genus: Casanori: Casanori, Jusmo: Jujusmo, Tamajusmo, Mizojusmo, Mikazukiki, Korekaete, Tsutsumimo, Kikougususa, Kyukougusa, Hitogususa: Homogusa, Hirohatohigususa, Umushimoe, Sakaki Sumiremo: Sumiremo, Hoshimamidro, Fushinashimidoro etc.).

  Cyanobacteria (Suizenjinori: Suizenjinori, Aoko, Nenju: Kawatake, Ishijelly, Hassai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium (Aiacio), etc.)

  Brown alga , Sanadagusa: Sanadagusa, Fukurimamiji, Common genus: Commongusa, Yahazugusa genus: Ezoyahaz, Yahazugusa, Ulaboshiyahaz, Jigamigusa genus: Jigamigusa, Umuchiwa genus, Urumus samma : Navarimo, Chasoumen: Motsukichasomen, Matsumo: Matsumo, Nagamatsumo: Nagamatsumo, Okinawa Mozuku: Okinawa Mozuku, Nisefu Mozuku: Niseft mozuku, Fumozuku genus: Futomozuku, Ishimozuku genus: Ishimozuku, Kuromo genus: Kuromo, Nisemozuku genus: Nisemozuku, Mozuku genus: Mozuku, Ishigeu genus: Ishige, Iroro, Ichimegasa genus: Ichimegasa, Kayarimitsu Cypridina, Urushigusa: Urushigusa, Keurushigusa, Tobacco gussa, Composita: Composita, Habasmodoki: Haberodoki, Habanori: Habanori, Habanori: Habanori, Commonbukuro, Ezobukuro, Ezobukurofufu Chishima fukuronori genus: Chishima fukuronori, Kagomenori genus: Kagomenori, Muraridori genus: Murachidori, shark genus: shark zusa, genus genus: lobster, genus genus: Yokojimari, mosquito Monoli genus: frog genus, fennel spider: fennel spider, vine genus: turmo, turtle genus: shark, genus Susime: sujime, genus misjikonbu: misjikonbu, atsubamisujikonbu, konbu genus: tsutsuarakonbu, kakijima konbu, onikombu, nakonbu Barracuda, Torocombu, Antokume, Antokume, Antarctic, Kajime: Kajime, Tsuruarame, Kurome, Kikuishikombu: Kikuishikonbu, Nejirumubu: Nejicombu, Kuroshiome: Kuroshiome, Nekoshikonbu, Aramei Genus: Ainu turtle, Chigaiso, Oniwakame Ezo-shige: Ezoi-shige, Yabane-moku: Yabane-moku, Rappa-moku: Rappa-moku, Joromoku: Ugano-moku, Jolo Mok, Hiemoku, Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawara, Tatsuri, Yatsumata Moku, Umitorano, Obamoku, Fusushimoku, Hahamokumoku , Ukimo: Ouuki, Burukimo: Burukimo, Kayamonori: Kayamonori, etc.).

  Red algae Genus: Cyprinus, Tsukumonori, Kamamohiranori, Benimozuku: Benimozu, Hosobenimozu, Kasamatsu Genus: Casamatsu, Fusanori: Fusanori, Nisefusanogeni: Nisefusanori, Sotagarahahi, Ragarata Genus: Tamaitadaki, Kaikekenori Genus: Kaginori, Kagikenori, Tengusa: Himetengususa, Hytengususa, Oobusa, Nambugususa, Ko La, Yohira, Kinusa, Hibirodo: Hibirodo, Himebiro, Isomumedo: Isommedo, Aedes, Aedes, Ryumons: Ryomonas, Amaranthus, Acacias Genus: Hosobanaminohana, Naminohana, Sangomodoki: Ragaramomodoki, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanokawa, Kainokawa , Sakuranori, Nikumukade, Tambanori, Tsurutsuru, Isonohana: Nuraksa, Cronouraxa, Oumkadenori, Hirakinto Genus: Astragalus, Matabou: Matabou, Kintoki: Chabokintoki, Kintoki, Pine tree, Rice, Scots pine, Pinus japonica, Kakateto: Oobakintoki, Ayufunori: Itofori, Naganobane, Funaburi, Fuso Genus genus: genus cricket, genus genus: red mosquito, black swordfish, mosquito genus, genus genus: genus genus, genus genus genus: genus genus genus : Chrysanthemum, Usuginu, Nikuhounoo: Nikuhounoo, Venusnago: Vennisnago, Suscabeni: Suscabeni, Okarugusa: Yamadagusa, Mirrin: Mirrin, Hoso Milin, Tosakanori: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomokka, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kaigaibaranori, Kizino-no-oh, Ogonori: Ogonori, Tsurushiramo, Shiramo, Oonogori, Mizookonori, Birch, Amanita genus: Hachijotensamodoki, Fushikenori: Fushikenori, Namiiwatake, Namiiwasita , Spinach, spinach genus: spinach, cedar genus: ikanoashi, hosoibonori, novonori, kurohaginansou: kurohaginansou, akabaginna Genus: Red-backed ginseng, Genicillus cerevisiae: Cyprinus genus, Madaragusa genus: Columbine, Etsukimadara, Taoyagisou: Genuine genus, Japanese cherry, Fukutsunagi genus: Fukutsunagi, Sugikonori, Hanaedari, Genus genus Anadalus, Uebubasae: Uebusca, Benifukuronori: Benifuronori, Fusutunagi: Fusutunagi, Himefushitsunagi, Hirohafushitsunagi, Otsunagisou: Hirawatsugisou, Usubawagunagi, Igis, Egis , Futogigi, Saeda: Saeda, Chilemomi: Chilemomi, Conophyllaceae: Habutaenori, Conohanori, Suzushironori, Usbeni: Usbeni, Hasujiginu: Hasujiginu, Nagakononohanori: Nagakonohanori, Sugiginu: Sugiginu, Abasbasginu, Heiusbanori: Kagiosbanori, Yareusbanori, Susiusbanori, Haiusubaniyaki, Usbanoyadoki Enasidadia, Shimadadia genus: Isohagi, Shimadadia, Dajimodoki: Dajimodoki, Euglena: Moroitogusa, Futoigusa, Makuri: Makri, Yanagiri: Hanayagi, Yuna, Yanaginori, Motsurezona, Vulture, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosozozanemo, Himegoke: Himegoke, Black-eyed moss, Hyodosig genus: Chrysanthemum odorus, Hyodosigusa, Usubahiodoshi, Isomegusa genus: Isomegususa, Suginassa genus: Suzynigusa, Isobachou genus: Isobasho, Fujimatsumo genus: Azalea genus Ideyukogome: Ideyukogome, Ochimomoku: Ochimomoku, Otogusa, Chinorimo: Chinorimo, Chisujinori: Chisujinori, etc.).

  Axial algae (Shajikumo, Shiratamamo, Hoshitsurimo: Hoshiturimo, Rikunotumusu, Flaskmo: Himeflask, Chaboflasmo, Tripera, etc.), yellow algae (Hikarimo: Hikarimo, etc.), etc.

  In addition, animal-derived materials include poultry crown extract, cow / human placenta extract, pig / cow stomach, duodenum or intestinal extract or degradation product thereof, pig / cow spleen extract or its Degradation products, extracts of porcine and bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin or their degradation products or their Derivatives, silk protein or its degradation products or their derivatives, porcine / cow blood cell protein degradation products (globin peptides), porcine / cow hemoglobin degradation products (hemin, hematin, heme, protoheme, heme iron, etc.), milk, casein or Decomposition products or derivatives thereof, skim milk powder or decomposition products or derivatives thereof, lactoferrin or decomposition products thereof, egg components, fish decomposition products, and the like.

(14) Microbial cultured metabolites Yeast metabolites, yeast extract, rice fermented extract, rice bran fermented extract, Euglena extract, lactic acid fermented milk powder or trehalose or its derivatives.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid, etc.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Bengala, black iron oxide, Gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan Derivatives, pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, Kaolin etc.

(18) Whitening agents p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone Derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, etc.

(19) Tyrosinase activity inhibitor Ascorbic acid or a derivative thereof, hydroquinone or a glycoside thereof, kojic acid or a derivative thereof, placenta extract, silk peptide, plant extract (mulberry, sugar beet, wallet, clam, mugwort, honeysuckle, yellowfin, dokudami , Matsuhodo, pearl barley, nettle, hop, hawthorn, eucalyptus, yarrow, altea, keihi, mandarin, hamamelis, yamaguwa, life-grass, bellflower, toshishi, sequel, giraffe, mao, nematode, doctus, psycho, boucu , Ogon, Peony skin, Peonies, Gennoshouko, Kakkon, Licorice, Pyrup, Aloe, Shouma, Safflower, Green tea, Black tea, Asen medicine, etc.).

(20) Melanin pigment reduction / decomposition Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, etc.

(21) Turnover promotion / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe, ougone) , Horsetail, gentian, burdock, shikon, carrot, hamamelis, hops, yellow lily, seaweed, sea bream, cypress, red snapper, achacha, hypericum, cucumber, red pepper, rosemary, parsley, etc.).

(22) Astringents Succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compounds), etc.

(23) Reactive oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydrogua cetelenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole , Propyl gallate, sesamol, sesamorin, gossypol, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (sesame cultured cells, amacha, hypericum, hamamelis, clove, melissa, birch, birch, sage, rosemary, minamitenmi, kina, agetsu, ginkgo, green tea, etc. ).

(26) anti-inflammatory agent ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, Glycyrrhetinic acid or its salt.

(27) Disinfectant / disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolysates thereof.

(28) Moisturizer Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, sodium lactate, etc.

(29) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, thio Glycolic acid and so on.

(30) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate, Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(31) Dye agent 5-aminoorthocresol, 2-monoamino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol, hydrochloric acid 2 , 41 diaminophenoxyethanol, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoaminophenol , N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylenediamine, paraamino Enol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium picramate, N, 'N-bis (4-aminophenyl) -2,5-diamino-1, -4-quinonediimine, 5 -(2-hydroxyethylamino) mono-2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorcresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate , Orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, Lanitroorthophenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, fluorologlucin, Gallic acid, resorcin, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozene-5'-sodium sulfonate, hematein and the like.

(32) Fragrance Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, bergamot Oil, Peru balsam essential oil, Boadrose essential oil, melamine essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaroe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary essential oil, Japanese mint essential oil, etc. Synthetic fragrances.

(33) Dye and Colorant Red Cabbage Dye, Red Rice Dye, Akane Dye, Anato Dye, Ikumi Dye, Turmeric Dye, Enju Dye, Krill Dye, Amber Dye, Caramel, Gold, Silver, Gardenia Dye, Corn Dye, Onion Dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(34) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin, etc.

(35) Nutritional fortifier Calcinated shellfish calcium, cyanocolabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron, etc.

(36) Dairy products Milk, cheese, fresh cream, butter, margarine, powdered milk, whey, condensed milk, etc.

  Others, moisturizers, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic and antibacterial agents, refreshing agents, stabilizers, emulsifiers, animal and vegetable proteins or their degradation products, animals and plants Saccharides or their degradation products, animal / plant glycoproteins or their degradation products, blood flow promoters, anti-inflammatory agents / antiallergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral cavity Lipase activity known to date, such as lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor of the present invention. An additive or synergistic lipase activity promoting action, moisturizing action, whitening action or anti-inflammatory action can be expected depending on the combination with an accelerator, a moisturizing agent, a whitening agent or a component having a contact dermatitis inhibitor.

  Further, the lipase activity promoter, moisturizer, whitening agent or contact dermatitis inhibitor of the present invention, skin external preparations or bath preparations, and food and drink dosage forms are arbitrary, ampules, capsules, powders, granules Pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, etc., quasi-drugs, skin / hair cosmetics or bath preparations, It can mix | blend and use for food-drinks.

  Specifically, for example, pharmaceutical preparations for internal and external use, lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, shampoos, rinses, hair treatments, hair creams , Pomade, hair spray, hair conditioner, permanent, hair art, hair dye, hair cosmetics such as hair growth and hair restorer, foundation, white powder, funny, lipstick, blusher, eye shadow, eyeliner, mascara, eyebrow, eyelash, etc. Make-up cosmetics, cosmetics for finishing nails, perfumes, bath preparations, toothpastes, mouth fresheners, gargles, liquid odors, deodorants, sanitary products, sanitary cotton, wet tissue It can also be used for other general foods and drinks.

  The sisal extract-containing lipase activity promoter of the present invention, moisturizer, whitening agent or contact dermatitis inhibitor, skin external preparation or bath preparation, and the method of addition to food and drink, may be added in advance, It may be added during the production, and may be appropriately selected in consideration of workability.

  Hereinafter, production examples, test examples, and formulation examples will be described. However, the present invention is not limited to these examples.

(Production Example 1)
Agave and sisal, 100 g each, were heated and extracted with purified water (about 80 ° C.) for about 6 hours, extracted at room temperature for a whole day and night, and then filtered to obtain an extract (dry solid content: about 0.4 to 1.6 weight) %) Is obtained.

(Production Example 2)
Agave, sisal, 100 g each was immersed in 50% ethanol solution, 50% 1,3-butylene glycol solution, 60% propylene glycol solution, or purified water (about 30 ° C.) and extracted at room temperature for 4 days, Filtration is performed to obtain about 1.0 kg of an extract (dry solid content: about 0.4 to 1.6% by weight).

(Test 1)
Evaluation of lipase activity promoting action In the lipolysis process, triacylglycerol is decomposed into fatty acid and glycerin. This degradation reaction is carried out by lipase, and thus the promotion of lipase activity is an indicator of fat reduction. In addition, activation of lipase is considered to involve a multi-step reaction via c-AMP in adipocytes. In this test, the extract obtained in Production Examples 1 and 2 was examined by measuring the lipase activity value using the quantitative reaction of lipase in human blood as an index.
(Test method)
a. Sample The extract obtained in Production Examples 1 and 2 of the present invention was prepared to 50% concentration and used for the test.
b. Skin extract 10 g of physiological saline was added to 1 g of guinea pig dorsal skin, extracted with ultrasound, and the supernatant obtained by centrifugation (12000 rpm, 15 minutes) was used as the skin extract.
c. Enzyme lipase Enzyme lipase (from porcine pancreas / Sigma) was adjusted to 0.2 units.
d. The test was performed according to the test method of Lipase Kit S (manufactured by Dainippon Pharmaceutical Co., Ltd.) using a mixed solution of 30% lipase activity measurement sample, 20% skin extract and 50% 0.2units lipase. In addition, as a control, purified water and various extraction solvent concentrations were used instead of the sample, and the control was set to 100% due to the difference in coloration from the blind in which the substrate was added after stopping the reaction for each sample and control. The lipase activity promotion rate (= lipase activity promotion action) was determined by the following formula (Equation 1).

(Test result) As shown in Table 1, it was confirmed that the sisal extract of the present invention has a lipase activity promoting action.

(Test 2) Moisturizing test (test method and evaluation method)
1) Using a skin stratum corneum moisture measuring device (high-frequency IMPERDANCE METER model IB 335, manufactured by IBS Co., Ltd.), the electrical conductivity of the skin was measured with an electrode having an outer diameter of 6 mm and a center diameter of 1 mm. The measurement site is on the forearm flexion side, and the electrical conductivity of the measurement site is measured in advance. Next, about 1 ml of each sample was applied to the measurement site, and after 30 seconds, the excess sample was removed, and immediately after that, the electrical conductivity was measured every 30 seconds for 19 minutes, and a moisture retention test was performed. In addition, the sample of the moisturizing test prepared the extract obtained in Production Examples 1 and 2 of the present invention to a concentration of 50% and used for the test. The test conditions were temperature: 22-24 ° C., relative humidity: 50-52%, and the results are shown in FIG.

(Test result) As shown in FIG. 1, it was confirmed that the sisal extract of the present invention has a moisturizing action as compared with purified water.

(Test 3) Moisturizing test (test method and evaluation method)
1) Using a transepidermal water loss measuring device (TEWAMETER TM210 COURAGE + KHAKA Elctonic GmbH Germany), apply 1 ml of each sample on the left forearm flexion side and wipe off after 30 seconds. After wiping, measurement was started about 10 minutes later using a transepidermal water loss measuring apparatus, and measurement was performed at intervals of 2 seconds for 1 minute and 30 seconds to obtain an average value of all values. Each blank was measured under the same conditions before sample application, and the difference before and after application was taken as the amount of moisture change. The results are shown in FIG. The test conditions were adjusted to temperature: 22-24 ° C. and relative humidity: 50-52%.

(Test results) As shown in FIG. 2, it was confirmed that the sisal extract of the present invention has a reduced moisture transpiration rate and has a moisturizing action as compared with purified water.

(Test 4) Melanin production inhibition test (test method and evaluation method)
a. B16 mouse melanoma cells were used as cells and culture condition cells. MEM medium (GIBCO BRL) containing 10% fetal bovine serum (GIBCO BRL) was used as the culture solution, and the culture was performed under conditions of 5% CO 2 and 37 ° C.
b. Sample As the sample for the moisturizing test, the extract (solid content concentration 0.5%) obtained in Production Examples 1 and 2 of the present invention was used for the test. As comparative samples, arbutin (Sigma) and ascorbic acid (Kishida Chemical) were prepared under the same conditions and used for the test.
C. Measurement 1 × 10 5 B16 melanoma cells were implanted in a 60 mm plastic petri dish and pre-cultured for 24 hours. Thereafter, the medium is replaced with fresh medium, and the sample is added. The cells were cultured for 3 days and then collected by trypsinization. Non-melanin substances were removed from the collected cells with 5% TCA, ether / ethanol mixture, anhydrous ether, then dissolved in 1NNaOH, 10% DMSO, and the absorbance at 420 nm was measured to determine the amount of melanin (melanin production inhibitory effect). It was. The results are shown in FIG.

(Test result) As shown in FIG. 3, it was confirmed that the sisal extract of the present invention has an equivalent or higher melanin production inhibitory action compared with arbutin and ascorbic acid, and can therefore be used as a whitening agent.

(Test 5) Contact Dermatitis Inhibition Test In the contact dermatitis reaction, when T lymphocytes sensitized by an antigen come into contact with the same antigen again, various lymphokines that activate macrophages and lymphocytes are released and inflammation occurs. Causes a reaction. Therefore, a substance that suppresses a series of inflammatory reactions that occur again against the same antigen can be expected to be used as a delayed-type allergic agent. In this test, a hydrophilic petrolatum ointment containing the extract obtained in Production Examples 1 and 2 was formulated, and paraphenylenediamine-induced contact dermatitis according to the method of Nakamura et al. (Nippon Pharmacology, 76,595 (1980)). The effect on the reaction was examined.

(Test method)
a. The sample extract was concentrated by distilling off the solvent under reduced pressure, and then a hydrophilic petrolatum ointment containing 10% by weight in terms of solid concentration was produced and used in a conventional manner.
b. Measurement of edema swelling rate First, 0.1 ml of 2.5% paraphenylenediamine / acetone: olive oil = 4: 1 (hereinafter referred to as PPD) is continuously applied to the shaved abdominal skin of a mouse (BALB / c female mouse: about 8 weeks old) for 3 days. After 5 days of application, 20 mg of the test drug was applied to the unilateral pinna three times at 1 hour intervals. One hour after the final application, the test drug was sufficiently wiped off, and 20 μl of PPD was applied to the auricle to cause the test drug. After 17 hours of induction, the test drug is applied again to the pinna raised twice at 1-hour intervals, then the test drug is thoroughly wiped 19 hours after the trigger, and both ears are punched with a certain area (diameter) 24 hours after the trigger. 5.0 mm) and weighed. In the determination, the swelling rate was measured from the difference in the weight of the untreated unilateral auricle, the inhibition rate of dermatitis was determined by comparison with the control group, and a contact dermatitis inhibition test was conducted. In the test, 8 to 9 mice were used.

(Test results) As shown in Table 2, it was confirmed that the sisal extract of the present invention has a contact dermatitis inhibitory action.

(Test 6) Safety test (1)
Japanese White Rabbit prepared by purifying the agave and sisal extracts obtained in Skin Primary Irritation Test Production Examples 1 and 2 with purified water so that the dry solids concentration is about 1.0 W / V%, and shaving the back. (Female, 3 mice per group, body weight around 2.3 kg) applied to the skin. Judgment was performed 24, 48, and 72 hours after application using the primary irritation scoring method with erythema and edema as indicators. The results were negative in all animals without any erythema or edema.

(Test 7) Safety test (2)
Hartley prepared by purifying the agave and sisal extract obtained in Production Examples 1 and 2 with purified water so that the dry solids concentration is about 1.0 W / V%, and shaving the flank, as in the cumulative skin irritation test. 0.5 ml / mouse was applied to the skin of a system guinea pig (female, 3 mice per group, body weight around 320 g) once a day, 5 times a week. Application was performed over 2 weeks and shaving was performed on the last application day of each week. Judgment was performed on the day of each application and the day after the last application by the scoring method of primary irritation using erythema and edema as indices. The results were negative in all animals without any erythema or edema over 2 weeks.

(Test 8) Safety test (3)
Similar to the acute toxicity test, the agave and sisal extract obtained in Production Examples 1 and 2 were vacuum-concentrated and dried, and the powder (dry solid content: about 1.0 W / V%) was fasted for 4 hours before the test. 2000 mg / kg was orally administered to ddy mice (male and female, 5 mice / group, 5 weeks old), and the onset and degree of toxic symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days, and no abnormalities were found in the results of dissection. Therefore, LD50 was determined to be 2000 mg / kg or more.

(Formulation examples) Various lipase activity promoters, moisturizers, whitening agents, contact dermatitis inhibitors, skin external preparations or bath preparations, food and beverage products According to the above evaluation results, the following formulation examples are shown. May be produced by a conventional method for producing each product, and only the blending amount is shown. The present invention is not limited to these.

(Formulation Example 1) Emulsion
weight%
1. Squalane 5.0
2. Olive oil 5.0
3. Jojoba oil 5.0
4). Cetyl alcohol 1.5
5. Glycerol monostearate 2.0
6). Polyoxyethylene (20) cetyl ether 3.0
7). Polyoxyethylene (20) soorbitan monooleate 2.0
8.1,3-Butylene glycol 1.0
9. Glycerin 2.0
10. A: Agave hot water extract B: Sisal hot water extract * Extract of either A or B 5.0
11. Perfume and preservatives Residue with 100 purified water

(Prescription Example 2) Shampoo
weight%
1. Lauryl sulfate triethanolamine 5.0
2. Sodium polyoxyethylene lauryl ether sulfate 12.0
3. 1,3-butylene glycol 4.0
4). Lauric acid diethanolamide 2.0
5. Edetate disodium 0.1
6). Sisal 50% 1,3-butylene glycol extract 10.0
7). Perfume and preservatives Residue with 100 purified water

(Formulation example 3) Body soap
weight%
1. Potassium laurate 15.0
2. Potassium myristate 5.0
3. Propylene glycol 5.0
4). Sisal 50% ethanol extract 5.0
5. Agave 50% propylene glycol extract 5.0
6). pH adjuster appropriate amount7. Preservative appropriate amount 8. Residue with 100 purified water

(Formulation Example 4) Hair Liquid
weight%
1. Ethanol 29.0
2. Polyoxypropylene butyl ether phosphate 10.0
3. Polyoxypropylene monobutyl ether 5.0
4). Triethanolamine 1.0
5. A: Agave 50% ethanol extract B: Sisal 50% ethanol extract * Extract of either A or B 3.0
6). Preservative appropriate amount 7. Residue with 100 purified water

(Prescription Example 5) Hair Tonic
weight%
1. Ethanol 40.0
2. Ethyl oleate 1.0
3. Polyoxyethylene (40) hydrogenated castor oil 2.0
4). A: Agave 50% ethanol extract B: Sisal 50% ethanol extract * Extract from either A or B 5.0
5. Residue with 100 purified water

(Formulation Example 6) Granule bath preparation
weight%
1. Sodium bicarbonate 60.0
2. Anhydrous sodium sulfate 30.0
3. Borax 3.0
4). A: Agave 50% 1,3-butylene glycol extract B: Sisal hot water extract * Extract of either A or B 7.0

(Formulation Example 7) Mentsuyu Solution Weight%
1. Soy sauce 80.0
2. Black vinegar 2.0
3. Glucose 15.0
4). Sodium glutamate 2.0
5. Sisal hot water extract powder 1.0

(Formulation example 8) Udon or buckwheat wt%
1. Wheat flour or buckwheat 96.0
2. Salt 1.0
3. Sisal hot water extract powder 3.0

(Formulation example 9) Bread weight%
1. Flour 91.0
2. Salt 0.7
3. Glucose 6.8
4). A: Agave hot water extract powder B: Sisal hot water extract powder * Extracted powder of either A or B 1.5

(Formulation example 10) Cookie weight%
1. Milk 65.0
2. Whole egg 14.0
3. Sugar 15.0
4). Consturch 4.1
5. Salt 0.4
6). Sisal hot water extract powder 1.5
7). Perfume

(Prescription Example 11) Beverage weight%
1. Glucose liquid sugar 33.0
2. Grapefruit juice 62.0
3. Agave hot water extract powder 2.5
4). Sisal hot water extract powder 2.0
5. Fragrance 0.5
6). Sour seasoning

(Formulation example 12) Gum weight%
1. Mentholmicron 31.0
2. Grapefruit flavor 65.0
3. Agave Hot Water Extract 2.0
4). Sisal hot water extract 2.0

(Prescription Example 13) Miso Soup Weight%
1. Tofu 45.0
2. Fried chicken 2.5
3. Carrot 5.0
4). Burdock 5.0
5. Red miso 15.0
6). A: Agave hot water extract powder B: Sisal hot water extract powder * Extracted powder of either A or B 5.0
7). Remaining bonito stock 100

(Test 9) Use effect test The effect at the time of actually using the skin external preparation or bath preparation of this invention was examined. The use test was conducted by 20 people aged 15-30 years old suffering from oily skin, acne and eczema, and each day, twice a day in the morning and at night, after the face was washed, the appropriate amount of the formula 1 emulsion was applied to the face for 3 months. It was done by doing. In addition, for 20 people (15-30 years old) who have similar skin diseases at the hairline of the scalp and hair, apply an appropriate amount of the hair to the scalp for 2 months after daily hair washing. A usage test was conducted.

  Furthermore, with regard to the bath preparation of Formulation Example 6, bath water in which an appropriate amount of bath preparation is dissolved once a day for 3 months for 20 people aged 15 to 30 who suffer from oily skin, acne and eczema. We took a bath and conducted a usage test. As a control, a product prepared by removing the plant extract of the present invention from an emulsion, hair art, or bath preparation was prepared in the same manner. Moreover, the evaluation method is performed according to the following criteria, and the results are as shown in Tables 3 to 5. The numerical values in the table represent the number of people. None of the patients complained of skin or scalp abnormalities during the period of use.

"Skin (scalp) disease improvement effect"
Effective: The oily skin, acne and eczema were improved.
Slightly effective: Slightly improved oily skin, acne and eczema.
Invalid: No change before use.

(Test results)
From the results of Tables 3 to 5, the use of the sisal extract-containing skin external preparation or bath preparation of the present invention was confirmed to have a good effect on the improvement of oily skin, skin diseases such as acne and eczema.

(Test 10) Use effect test The effect at the time of actually using the foodstuff containing the plant extract of this invention was examined. The use test is a panel of 10 people aged 15 to 30 who suffer from oily skin, acne, eczema, and once a day at breakfast, be sure to drink only 3 cups of prescription example 13 (about 150 cc) for 3 months. I got it.

  As a control, what was prepared by removing the plant extract of the present invention from miso soup by the same method was used. The evaluation method is based on the following criteria, and the results are as shown in Table 6. The numerical values in the table represent the number of people. None of the patients complained of skin or scalp abnormalities during the period of use.

"Skin disease improvement effect"
Effective: The oily skin, acne and eczema were improved.
Slightly effective: Slightly improved oily skin, acne and eczema.
Invalid: No change before use.

(Test results)
From the results in Table 6, it was confirmed that the use of the sisal extract-containing food or drink of the present invention had a good effect on the improvement of oily skin and skin diseases such as eczema and acne.

(Test 11) Use effect test The effect when actually using the skin external preparation or bath preparation of the present invention was examined. The use test was conducted by 20 persons aged 5 to 50 years who suffer from inflammatory skin diseases such as rough hands, kayumi, and redness, or dry / grown skin, and each day, twice daily in the morning and at night, after the face wash, the appropriate amount of the emulsion of Formulation Example 1 Was applied to the face for 3 months. In addition, for 20 people (3 to 45 years old) who have similar skin diseases on the hairline of the scalp and hair, apply an appropriate amount of the hair artnic of Formulation Example 5 to the scalp over 3 months after daily hair washing. A usage test was conducted.

  In addition, for the bath preparation of Formulation Example 6, for 20 people aged 1 to 65 who suffer from inflammatory skin diseases such as rough hands, kayumi and redness, or dry / grown skin, it is always once a day for 3 months. A use test was conducted after bathing in a bath water in which an appropriate amount of bath agent was dissolved. As a control, a preparation prepared by removing the various plant extracts of the present invention from an emulsion, hair art, and bath preparation was prepared in the same manner. The evaluation method is performed according to the following criteria, and the results are as shown in Tables 7 to 9, and the numerical values in the table represent the number of people. None of the patients complained of skin or scalp abnormalities during the period of use.

"Skin (scalp) disease improving effect" or "Moisturizing effect"
Effective: Inflammatory skin diseases such as rough hands, kayumi and redness, or dry / cracked skin was improved.
Slightly effective: inflammatory skin diseases such as rough hands, kayumi, and redness, and dry / grown skin were slightly improved.
Invalid: No change before use.

(Test results)
From the results of Tables 7 to 9, the use of the sisal extract-containing skin external preparation or bath preparation of the present invention has an effect of improving inflammatory skin diseases such as rough hands, kayumi and redness, or a moisturizing effect on dry and rusted skin. A good effect was confirmed.

(Test 12) Use effect test The effect at the time of actually using the foodstuff of this invention was examined. The use test is a panel of 10 people aged 5 to 50 years who suffer from inflammatory skin diseases such as rough skin, kayumi, redness, dryness, and rustling skin. About 50g) was eaten and consumed for 3 months.

  As a control, a bread prepared by removing the plant extract of the present invention from bread was prepared in the same manner. Moreover, the evaluation method is performed according to the following criteria, and the results are as shown in Table 10. The numerical values in the table represent the number of people. No one complained of any abnormality during the period of use.

"Skin (scalp) disease improving effect" or "Moisturizing effect"
Effective: Inflammatory skin diseases such as rough hands, kayumi and redness, or dry / cracked skin was improved.
Slightly effective: inflammatory skin diseases such as rough hands, kayumi, and redness, and dry / grown skin were slightly improved.
Invalid: No change before use.

(Test results)
From the results of Table 10, the use of the sisal extract-containing skin food or drink of the present invention has a good effect of improving inflammatory skin diseases such as rough hands, kayumi, and redness, or giving a moisturizing effect to dry, rusted skin. confirmed.

It is the figure which showed the epidermis horny layer moisture content of the sisal extract of this invention. It is the figure which showed the water | moisture-content change amount using the transepidermal water loss amount measuring apparatus for the sisal extract of this invention. It is the figure which showed the melanin amount of the sisal extract of this invention.

Claims (5)

A lipase activity promoter comprising an extract of sisal. A humectant containing an extract of sisal. A whitening agent characterized by containing an extract of sisal. Inhibition of skin or scalp fat or prevention or treatment of skin disease of oily skin, characterized in that any one or more of the lipase activity promoter, moisturizer, and whitening agent according to claims 1 to 3 are blended. Or a skin external preparation or bath preparation used to reduce excess fat in the body . Inhibition of skin or scalp fat or prevention or treatment of skin disease of oily skin, characterized in that any one or more of the lipase activity promoter, moisturizer, and whitening agent according to claims 1 to 3 are blended. or food and drink used to reduce excess fat in the body.