KR20130039470A - Method for extracting ginseng extracts by steamming with rice - Google Patents

Method for extracting ginseng extracts by steamming with rice Download PDF

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KR20130039470A
KR20130039470A KR1020110104041A KR20110104041A KR20130039470A KR 20130039470 A KR20130039470 A KR 20130039470A KR 1020110104041 A KR1020110104041 A KR 1020110104041A KR 20110104041 A KR20110104041 A KR 20110104041A KR 20130039470 A KR20130039470 A KR 20130039470A
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South Korea
Prior art keywords
ginseng
white rice
extract
ginseng extract
extracting
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KR1020110104041A
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Korean (ko)
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김동현
류권렬
이옥찬
염명훈
조준철
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(주)아모레퍼시픽
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Publication of KR20130039470A publication Critical patent/KR20130039470A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/51Concentration
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/25Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258Panax (ginseng)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • A23V2250/2124Ginseng

Abstract

The present invention provides a method for extracting ginseng extract using a white rice extract method comprising a process of steaming ginseng with white rice and an external skin composition and health food composition comprising the ginseng extract thereby. Ginseng extract extracted by the extraction method according to the present invention, the cytotoxicity is reduced, the effect of alleviating skin irritation is recognized.

Description

Extraction method of ginseng extract using bleaching method {METHOD FOR EXTRACTING GINSENG EXTRACTS BY STEAMMING WITH RICE}
The present invention relates to a skin external preparation composition and a health food composition comprising a ginseng extract extracted by the method of whitening and ginseng extract extracted by the method.
Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi ginseng and has been used in Korea, China, and Japan for more than 2,000 years, and has been used to prevent disease and extend life. Known efficacy and effects of ginseng to the central nervous system, anticarcinogenic effect, anticancer activity, immune function regulation, antidiabetic effect, liver function anti-inflammatory effect, cardiovascular disorders improvement, anti-arteriosclerosis, blood pressure control action, menopausal Disability improvement, effects on osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effect.
Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground parts of ginseng. Especially, the composition and composition of ginsenosides are different depending on the parts such as ginseng root (root), ginseng leaf and ginseng fruit. It is known.
Recently, as consumers' interest in natural cosmetics has increased and many cosmetics using herbal ingredients have been released, ginseng is also being studied as a plant material having important skin effects of cosmetics, but most of them are extracts or ginseng roots of ginseng root. Only the side components and ginseng polysaccharides are utilized, and the efficacy of various components of ginseng such as ginseng fruit is insufficient. In addition, the conventional method of obtaining an extract from ginseng has a problem in that it is unsuitable for lowering cytotoxicity while maintaining the pharmacological components of ginseng.
Patent Application Publication No. 10-2011-0058001
In order to solve the above problems, the present invention is to reduce the cytotoxicity of ginseng, and to obtain a ginseng extract using the whitening method with less skin irritation and less skin irritation including the ginseng extract extracted by the above method It is an object of the present invention to provide a healthy food composition having excellent skin whitening composition and ginseng-specific ingredients.
In order to solve the above object, the present invention provides a method of extracting ginseng extract using the white rice steam method comprising the process of steaming ginseng with white rice.
In one embodiment of the present invention, the ginseng may be one or more selected from the group consisting of ginseng root, ginseng fruit, ginseng flower, ginseng seed, ginseng leaf and ginseng stem.
In one embodiment of the present invention, the process of steaming ginseng with white rice, may include a step of exposing to high temperature steam or a step of immersing in water and heating.
In one embodiment of the present invention, the extraction method, after the process of steaming ginseng with white rice, may further comprise the step of extracting ginseng extract.
In one embodiment of the present invention, the process of extracting the ginseng extract may be by solvent extraction using water, an organic solvent or a mixture thereof.
In one embodiment of the present invention, the organic solvent may be one or more selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform.
In one embodiment of the present invention, the extraction temperature of the process of extracting the ginseng extract may be 10 to 80 ℃.
In one embodiment of the present invention, the extraction time of the process of extracting the ginseng extract may be 6 to 24 hours.
In addition, the present invention provides a skin external composition comprising a ginseng extract prepared by the extraction method.
In addition, the present invention provides a health food composition comprising a ginseng extract prepared by the extraction method.
Ginseng extract extracted by the extraction method according to the present invention, the cytotoxicity is reduced, the effect of alleviating skin irritation is recognized. In addition, the external preparation composition for skin containing the extract extracted by the above method exhibits excellent skin whitening efficacy, and can be used in various fields, such as cosmetics, the health functional composition comprising the ginseng extract extracted by the method is less toxic and blood circulation It may have the effect of improving immunity and improving immunity.
Hereinafter, preferred embodiments of the present invention will be described in detail in order to facilitate the present invention by those skilled in the art.
The present invention provides a method of extracting ginseng extract using the white rice steam method comprising steaming ginseng with white rice. Through the white rice steaming process that steams together with white rice, it removes the toxicity contained in ginseng and alleviates the skin irritation generated during skin application. Therefore, the ginseng extract prepared according to the extraction method of the present invention is low in cytotoxicity and skin irritation.
The ginseng may be one or more selected from the group consisting of ginseng root, ginseng fruit, ginseng flower, ginseng seed, ginseng leaf and ginseng stem.
The ginseng berry (Ginseng berry) is the fruit part of the ginseng having a component and composition different from the ginseng root in the ground portion of the ginseng. The ginseng seed may be any one of natural ginseng seed, seed meal and oil squeezed from ginseng seed. The ginseng leaves contain about 10-13% of saponins, which have a similar structure and pharmacological effect as ginseng saponins, 2 to 3 times more than ginseng root.
The process of steaming the ginseng together with the white rice may include a step of exposing to high temperature steam or a step of heating by dipping in water. For example, the process of steaming ginseng with white rice may be a process of cooking rice with white rice.
The extraction method may further include a process of extracting ginseng extract after steaming ginseng with white rice. The process of extracting ginseng extract in the present invention may be carried out through conventional methods known in the art. For example, water or an organic solvent is added to steamed ginseng with white rice, extracted by reflux extraction, and the residue and the filtrate are separated by filtration and centrifugation, and the filtrate is concentrated under reduced pressure to obtain a ginseng extract. have.
In addition, the extraction method according to the present invention may further include a process of extracting ginseng extract through solvent extraction, after the process of steaming ginseng with white rice. The kind of solvent used at the time of solvent extraction is not particularly limited, and may be, for example, water, an organic solvent or a mixed solvent of water and an organic solvent. Specifically, the solvent may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water. The solvent may be ethanol, more specifically 80% ethanol.
Extraction conditions during solvent extraction, the extraction may be carried out for 10 to 80 ℃ temperature and / or 6 to 24 hours. Although the extraction conditions are not particularly limited, the extraction efficiency may be lowered or the composition may be changed outside the extraction temperature and extraction time.
For extracts obtained through solvent extraction for ginseng, additional processing may be applied. For example, the extract obtained through solvent extraction may be cooled or heated at room temperature, and then filtered to obtain a liquid, and may be subjected to additional solvent evaporation, spray drying or lyophilization.
Ginseng extract prepared by the extraction method according to the present invention has the advantage that the cytotoxicity is significantly reduced, and at the same time shows an excellent skin whitening effect.
Accordingly, the present invention provides a composition for external application for skin containing ginseng extract obtained through the extraction method. Specifically, the external preparation composition for skin containing ginseng extract extracted through the process of steaming herbal medicines with white rice is recognized for reducing toxicity and alleviating skin irritation.
In the present invention, the external preparation composition may be a cosmetic composition, and more specifically, may be a composition for skin whitening. The ginseng extract contained as an active ingredient in the present invention inhibits the production of melanin, thereby providing an excellent skin whitening effect. The content of the ginseng extract contained in the cosmetic composition is not particularly limited. For example, the content of the ginseng extract, based on the total weight of the composition may be 0.001 to 90% by weight, specifically, may be 0.01 to 30% by weight, more specifically 0.1 to 10% by weight. The content range of the ginseng extract is to select a preferred range that can improve the whitening effect, when the content is lower than the above range, the skin whitening effect is not sufficient, the efficiency of the whitening effect due to the addition of the active ingredient is lowered when the range is exceeded do.
When formulating the external composition for skin according to the present invention in the form of cosmetics, soft cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, sun cream, cleansing foam, cleansing water, powder, essence, It may be formulated in the form of a foundation, makeup base or pack, etc., and the formulation is not particularly limited.
In addition, the present invention provides a health food composition comprising a herbal component extract using the whitening method according to the above. Specifically, the health food composition comprising the ginseng extract extracted through the process of steaming the herbal medicine with white rice, the pharmacological effect, toxicity reduction and stimulation alleviating intrinsic effect of ginseng is recognized.
Health food composition of the present invention preferably comprises 0.001 to 10% by weight of ginseng extract. If the ginseng extract is less than 0.001% by weight, the efficacy of ginseng does not appear sufficiently, and when the ginseng extract exceeds 10% by weight, the efficiency of the efficacy due to the addition of the active ingredient is lowered.
In the health food composition of the present invention, the composition may be in a liquid or solid formulation, tablets, capsules, soft capsules, pills, granules, beverages (drinks), diet bars, chocolate, caramel formulations or confectionary formulations The formulation is not particularly limited. The health food composition of the present invention, the health food composition may contain an excipient, sugars, flavorings, pigments, fats and oils, proteins and the like as necessary in addition to the active ingredient.
The present invention is described in more detail through the following implementation. However, the examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.
Example 1 Preparation of Ginseng Root Extract Using White Rice
4 l of water was added to 2 kg of white rice and heated on a high heat. By the time the rice started coming up, 300 g of ginseng roots (1.8 kg in total) wrapped in burlap porcelain were placed in the center of white rice and heated on low heat for about an hour. After heating, ginseng fruit, ginseng flower, ginseng seed, ginseng leaves and ginseng stems were collected, 9 liters of an 80% ethanol aqueous solution was added, extracted three times under reflux, and then deposited at 15 ° C. for 1 day. Then, the residue and the filtrate were separated by filtration of the filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 134 g of ginseng extract.
Example 2 Preparation of Ginseng Fruit Extract Using White Rice
Except for using ginseng fruit instead of the ginseng root in Example 1 was prepared in the same manner as in Example 1.
Example 3 Preparation of Ginseng Flower Extract Using White Rice
Except for using ginseng flower instead of ginseng root in Example 1 was prepared in the same manner as in Example 1.
Example 4 Preparation of Ginseng Seed Extract Using White Rice
Except for using ginseng seed instead of ginseng root in Example 1 was prepared in the same manner as in Example 1.
Example 5 Preparation of Ginseng Leaf Extract Using White Rice
Except for using ginseng leaves instead of the ginseng root in Example 1 was prepared in the same manner as in Example 1.
Example 6 Preparation of Ginseng Stem Extract Using White Rice
Except for using ginseng stem instead of ginseng root in Example 1 was prepared in the same manner as in Example 1.
[Comparative Examples 1 to 6] Preparation of General Ginseng Extract
Ginseng root, ginseng fruit, ginseng flower, ginseng seed, ginseng leaf and ginseng stem each 300 g (1.8 kg in total) was put 9 l of 80% ethanol aqueous solution, extracted three times under reflux, and then immersed at 15 ℃ for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation of the filter cloth, and the filtrate was concentrated under reduced pressure to obtain 125 g of ginseng extract. As a result, Comparative Examples 1 to 6 (ginseng root, ginseng fruit, ginseng flower, ginseng seed, ginseng leaf and ginseng stem) were obtained.
 Experimental Example 1 Cell Viability Test
Cell viability of the ginseng extract extracted by Examples 1 to 6 was compared. Examples 1 to 6 are the case of extracting ginseng components using the whitening method according to an embodiment of the present invention, as described above Comparative Examples 1 to 6 is the case of treating the ginseng extract not using the whitening method. In addition, the case of treating the remaining components after extracting the ginseng extract in Examples 1 to 6 were Comparative Examples 7 to 12, respectively, and the case of steaming less than white rice as Comparative Example 13. After each sample was treated for each concentration, the degree of proliferation of the cells was measured to compare the cell viability of the samples according to the Examples and Comparative Examples.
0 ppm 5 ppm 10 ppm
Example 1 100 117 126
Example 2 100 111 120
Example 3 100 114 124
Example 4 100 110 121
Example 5 100 112 119
Example 6 100 111 119
Comparative Example 1 100 98 89
Comparative Example 2 100 89 71
Comparative Example 3 100 86 79
Comparative Example 4 100 90 75
Comparative Example 5 100 91 80
Comparative Example 6 100 87 72
Comparative Example 7 100 60 45
Comparative Example 8 100 71 55
Comparative Example 9 100 73 61
Comparative Example 10 100 83 51
Comparative Example 11 100 77 63
Comparative Example 12 100 81 63
Comparative Example 13 100 102 105
Referring to Table 1, the examples compared to Comparative Examples 1 to 6, it can be seen that the cell viability is significantly increased depending on the concentration of the extract treatment. In addition, Comparative Example 7, which is a case of treating the remaining components after extracting the ginseng root in Example 1, was found to significantly decrease the cell viability according to the treatment concentration. In the case of Comparative Example 13, which is a case of steaming less than white, no difference in cell viability was found depending on the treatment concentration.
Through the results in Table 1, it can be confirmed that the toxic components were removed while the ginseng components are subjected to the bleaching method according to the present invention.
Experimental Example 2 Measurement of Melanin Inhibition Effect Using Pigment Cells
Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) in DMEM (Eagle's minimal essential medium) 10% fetal placental serum, 100 nM 12 -O-tetradecanoylphorbol-13-acetate and 1 nM cholera toxin were incubated at 37 ° C. and 5% CO 2 . The cultured Mel-Ab cells were separated with 0.25% trypsin-EDTA, the cells were incubated at a concentration of 10 5 cells / well in a 24-well plate, and each test substance was added for 3 consecutive days from the second day. And incubated.
As a test substance, ginseng extract using hydroquinone, the ginseng extract of Comparative Examples 1 to 6, and the white rice of Examples 1 to 6 were used at a concentration of 10 ppm, respectively. At this time, the hydroquinone was used as a positive control group. Then, the culture solution was removed, washed with PBS (Phosphate Buffered Saline; phosphate buffer solution), and the cells were dissolved with 1 N sodium hydroxide and absorbance was measured at 400 nm. The measured absorbance was calculated according to Equation 1 below to calculate the melanin inhibition rate is shown in Table 2 (Dooley's method).
[Equation 1]
Figure pat00001
Test substance Melanin production inhibition rate (%)
Untreated (control) 100
Comparative Example 1 89.9
Comparative Example 2 92.5
Comparative Example 3 81.1
Comparative Example 4 94.6
Comparative Example 5 92.9
Comparative Example 6 92.4
Example 1 42.7
Example 2 48.9
Example 3 40.8
Example 4 49.9
Example 5 50.2
Example 6 48.4
Hydroquinone (positive control) 41.1
As shown in Table 2, it can be seen that the ginseng extract using the whitening method according to the present invention shows a melanin production inhibition rate similar to that of hydroquinone which is a known whitening substance.
Experimental Example 3 Irritation Test
The irritation of kojic acid, a known whitening substance, and the ginseng extracts of Examples 1 to 6 were compared. Specifically, 15 panelists sensitive to irritation such as stinging, burning, etc. were tested for the degree of irritation such as stinging, itching, and eye irritation.
Subjects were rubbed with kojic acid (purchased through kojic acid, YM chemical) and 0.5 ml each of the herbal extracts of Examples 1 to 6 randomly applied to the left and right, and scored between 0 and 3.0 in units of 0.1 points. The results are shown in Table 3 below.
<Evaluation Criteria>
0 to 0.4: no stimulation
0.5 to 1.0: slightly irritating
1.1 to 2.0: moderate stimulation
2.1 to 3.0: severe irritation
Kojic acid Example 1 Example 2 Example
3
Example
4
Example
5
Example
6
Stinging 0.85 0.25 0.20 0.30 0.34 0.28 0.30
Burning 0.50 0.53 0.50 0.52 0.56 0.50 0.52
Average 0.68 0.39 0.35 0.41 0.45 0.39 0.41
Referring to Table 3, in the case of kojic acid, there was a feeling of tingling and burning, and there was a sense of irritation that could be felt slightly. On the other hand, the ginseng extract using the white rice used in the present invention has a slight irritation in the hotness, but it was found that there is no irritation on average because it is hardly felt.
Therefore, the ginseng extract using the white rice used in the present invention has a little irritating feeling as a substance different from kojic acid, it can be confirmed that it can provide better usability.
Experimental Example 4 blood circulation improvement test-NO production ability in human umbilical vein endothelial cells (HUVEC)
Endothelial nitric oxide synthase (eNOS) is present in human vascular endothelial cells, and its activity is increased to produce NO (nitric oxide) to expand blood vessels and promote blood circulation. After culturing human vascular endothelial cells, the amount of NO produced by treating white rice ginseng extract (Examples 1 to 6) and general ginseng extracts (Comparative Examples 1 to 6) in the medium was compared.
Endothelial cells were attached to gelatin coated 24-well plates at a density of 2.5 × 10 4 cells / well. Cells were incubated for 12 hours in growth medium. Vascular endothelial cells were pretreated for 12 hours in the group treated with whitening ginseng extract and general extract (Example, Comparative Example), no treatment group. It was then treated for 30 minutes with 10 μmol / L DAF-FM diacetate (Molecular Probe, OR) in M199 medium without FBS at 37 ° C. Since vascular endothelial cells were washed three times with M199 medium without FBS, placed in a parallel plate flow chamber and stimulated with light separated from a mercury lamp. The excitation wavelength was 488 nm and fluorescence was performed at 515 nm when NO was bound to DAF.
As can be seen in Table 4, the ginseng extract of Example 1 according to the present invention showed a degree of NO production of 1300% ~ 2000% compared to the untreated group at a concentration of 100 ㎍ / ml, the general extract of Comparative Example 1 The NO production level was 100-200% at 100 µg / ml.
NO production rate (relative to no treatment group)
Example 1 1300-2000%
Example 2 1200-1800%
Example 3 1500-2500%
Example 4 1000-1800%
Example 5 1200-1500%
Example 6 800-1000%