KR20110062159A - Cosmetic composition containing panax ginseng callus extracts for skin whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Panax Ginseng callus extract for skin whitening is provided to suppress tyrosinase activity without cytotoxicity. CONSTITUTION: A cosmetic composition for skin whitening contains Panax Ginseng callus extract. A cosmetic composition for antioxidation contains Panax Ginseng callus extract. The callus is induced in a MS medium, WHITE medium, B5 medium, or R2 medium containing growth regulator. The growth regulator is IAA(3-indoleacetic acid), IAld(3-indoleacetaldehyde), IPA(3-indolepyruvic acid), IAN(3-indoleacetonitrile), IAE(ethyl ester of indoleacetic acid), kinetin, BA(benzoic acid), or CPPU(N-(2-chloro-4-pyridyl)-N'-phenylurea). The cosmetic composition is manufactured in the form of skin softener, skin toner, astringent, lotion, milk lotion, nutrition lotion, massage cream, moisture cream, essence, pack, soap, shampoo, cleansing foam, cleansing lotion, body lotion, pressed powder, or eye shadow.

Description

Cosmetic composition containing Panax Ginseng Callus Extracts for skin whitening}

The present invention relates to a cosmetic composition for skin whitening containing Panax Ginseng callus extract, more specifically, the camphor ginseng extract of the present invention inhibits melanin production and inhibits tyrosinase activity, antioxidant activity It can be usefully used as a cosmetic composition for skin whitening because it is excellent and shows little cytotoxicity.

Skin is a very important tissue that has direct contact with the external environment, protects the human body and has biochemical and physical functions. This tissue is largely divided into three parts: the epidermis, the dermis, and the hypodermis. Melanin is a pigment in the epidermal layer of the skin, made from melanocytes in the basal layer of the epidermis and migrates to epidermal cells called keratinocytes. There are no enzymes in the body that break down melanin, but they are removed by falling off the skin as keratinocytes fall off the epidermis.

In the melanin synthesis process, the melanocyte vesicles in melanocytes are first converted into tyrosine through dopa (DOPA) by an enzyme called tyrosinase and then converted into dopaquinone (DOPAquinone). The enzymatic reaction produces melanin, a dark brown copolymer, via dopachrome. The melanin thus produced is transferred to keratinocytes through the melanosomes, where it is lost to the surface of the skin along with the keratinization process for about 28 days.

Melanin absorbs the light energy of sunlight UV to protect skin organs under the dermis from UV damage, and it protects the skin from external harmful factors such as trapping harmful oxygen and free radicals generated in skin skin. Play a role. In addition, the skin color of a person is determined by the amount of melanin in the skin cells. People with high melanin pigments have brown or black skin, while those with low melanin pigments have white skin.

However, abnormally low production of melanin causes skin lesions such as vitiligo. On the contrary, if melanin is not synthesized excessively due to daylight, hormonal changes, inflammation, or drugs, or the keratinization is not performed smoothly, the skin is damaged completely. In addition to the skin pigments are deposited to form blemishes, freckles, and even from these lesions can cause skin cancer. Therefore, in order to prevent such pigmentation phenomenon, it is necessary to inhibit a part of melanin production process to reduce melanin production.

Today, Asian women prefer white and clean skin like white jade and make it an important standard of beauty. Therefore, the development of whitening agent for treatment of abnormal skin pigmentation and satisfying beauty needs is being actively developed.

At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, whitening agents using tocopherols and vitamins used to reduce the melanin pigment are known to have a slight decolorizing effect of the melanin pigment. Therefore, attention is being paid to inhibitors that inhibit the production of melanin by inhibiting the activity of tyrosinase.

In the conventional cosmetic field, as a whitening component, for example, substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, hydroquinone, vitamin-C (L-Ascorbic acid) and Their derivatives and various plant extracts have been used.

However, the stability in the prescription system is poor, the color is degraded, or the use thereof is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems. Kojic acid inhibits enzymatic activity by adsorbing copper ions present in the active site of tyrosinase, but has been discontinued as a raw material for cosmetics because of its instability, skin side effects and recent animal experiments. Vitamin-C and its derivatives are difficult to use as raw materials for cosmetics due to their oxidative instability. Hydroquinone has excellent whitening effect on the skin but causes allergy, toxicity to melanocytes, and permanent depigmentation to the skin. It is highly irritating to skin, and is recently prescribed as a carcinogenic substance, so its use is banned and only a limited concentration is allowed in each country. In addition, arbutin is a derivative in which glucopyranoside is combined with hydroquinone, and has little side effects when using hydroquinone and inhibits the synthesis of melanin pigments without toxicity to humans, thereby increasing melanin pigmentation. Applicability has been suggested as a therapeutic agent for skin diseases, but it has a disadvantage of being partially degraded by skin enzymes.

In addition to these whitening substances, research has been conducted to find whitening active ingredients in natural products, especially plants. Among them, white skin (Japanese Patent Application Laid-Open No. 55-44375, Small 64-26507, Small 64-83009, Pyeong 1-25687 No. 5-139950, Korean Patent Publication Nos. 92-002109, 97-021273, Licorice (Japanese Patent Laid-Open No. 60-214721, No. 60-214728, No. 63-23809, No. 64- 63506, Hei 1-149706, and Korean Patent Nos. 92-002109, 97-025601), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Laid-Open Patent No. 92-002111), Cinnamon Patent Nos. 63-30403, Hep. 5-139954), Gosam (Japanese Patent Laid-Open No. 64-26507 and Korean Patent Laid-Open No. 92-002110), Brown root (Japanese Laid-Open Patent Nos. 60-214727, Small 64-16709 ), Angelica (Japanese Patent Laid-Open No. 56-92211, Hei 4-26610), Mokdanpi (Japanese Laid-Open Patent No. 60-214721, No. 61-50915, No. 61-246109), Banha (Japanese Laid-Open Patent Publication) Pyeong 2-207028, Korea Publication No. 96-033442), aloe (Japanese Patent Laid-Open No. 52-44375, Hei 2-207030, Hei 5-139950) and many plant extracts and herbal extracts act on tyrosinase to produce melanin Although it has been found to suppress the, they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration, etc., and still does not show an excellent effect.

The inventors of the present invention have completed the present invention by revealing that camphor ginseng callus extract reduces melanin production, inhibits tyrosinase activity, has an antioxidant effect, can be useful as a composition for skin whitening.

Therefore, an object of the present invention is to provide a whitening composition comprising camphor ginseng callus extract.

In addition, another object of the present invention is to provide an antioxidant composition comprising camphor ginseng callus extract.

The present invention may include the following means.

The cosmetic composition for skin whitening according to the present invention contains Panax Ginseng callus extract.

Antioxidant cosmetic composition according to another embodiment of the present invention contains camphor ginseng extract.

The callus is derived from one medium selected from MS medium, WHITE medium, B5 medium, R2 medium, the medium includes a growth regulator, the growth regulator is IAA (3-indoleacetic acid), IAld (3- indoleacetaldehyde (IPA), 3-indolepyruvic acid (IPA), 3-indoleacetonitrile (IAN), ethyl ester of indoleacetic acid (IAE), kinetin (kinnetin), benzoic acid (BA), or CPPU (N- (2-chloro-4-) pyridyl) -N'-phenylurea) is characterized in that formed one.

The cosmetic composition, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, It is characterized by taking one of the formulations selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow.

The cosmetic composition for skin whitening of the present invention contains camphor ginseng callus extract as an active ingredient, inhibits the production of melanin by B16 melanoma cells, and has an effect of inhibiting tyrosinase activity. In addition, there is also an antioxidant activity, in particular because it shows little cytotoxicity, it can be usefully used as a cosmetic composition for skin whitening.

The present invention provides a cosmetic composition for skin whitening containing camphor ginseng callus extract as an active ingredient. The whitening effect of the cosmetic composition can be confirmed in the following experimental example by inhibition of melanin production, inhibition of tyrosinase activity, the present invention also provides an antioxidant composition containing camphor ginseng callus extract.

Panax Ginseng (Panax Ginseng) used in the present invention refers to wild ginseng planted and raised, also called camphor or camphor ginseng (長 腦 山 蔘), elder (長蘆), mountain goat. Seeds of wild ginseng were harvested and seeded in deep mountains and grown wild. The camphor is named because it has a long brain that connects the stems and roots, but it is difficult to distinguish the general public. It grows well in moist areas under shady birch or lacquer trees in deep mountains, and it is only half-shaded. In general, wild ginseng is very valuable because the seeds do not sprout well and often disappear from the ground, and even if they are grown, they do not last for several years and are prone to rotting. It has almost the same effect as wild ginseng in shape and medicinal effect.

As used herein, the term callus is an amorphous tissue or cell mass that has lost its ability to cause normal organogenesis or tissue differentiation, and is mostly flow cytoplasm, and the tissue cut out of the plant contains auxin or cytokinin. Occurs when incubated in a plant, injuring a plant, or by treating a plant's epithelium with auxin. Callus can be derived from any part of the plant, such as leaves, stems, roots, rhizomes, fruits, seeds, through plant cell culture, especially tissue culture, and has the characteristic that it can be continuously produced by subculture.

Induction of callus can be carried out through conventional methods known in the art. Preferably, the plant tissue or fragments thereof are cultured in tissue culture medium. As the medium, MS (Murashige & Skoog) medium, WHITE medium, B5 medium, R2 medium and the like can be used. In this case, it is preferable to select a medium suitable for the plant to be cultivated in consideration of the species of the plant, the age of the plant, the type of the cultivation organ and its maturity. It is also desirable to add growth regulators to the medium. The growth regulator, but is not limited to, IAA (3-indoleacetic acid), IAld (3-indoleacetaldehyde), IPA (3-indolepyruvic acid), IAN (3-indoleacetonitrile), IAE (ethyl ester of indoleacetic acid) and Same auxin and its derivatives (e.g. 2,4-D (2,4-dichlorphenoxy acidic acid), NAA (naphthaleneacetic acid)) and kinetin, BA (benzoic acid), CPPU (N- (2) cytokinin and derivatives thereof, such as -chloro-4-pyridyl) -N'-phenylurea). In addition, natural extracts that act on differentiation and proliferation of cells may be further added to the medium. Preferred callus induction and cultivation methods are as follows: First, a part of the plant tissue is isolated and sterilized, followed by organic components such as carbohydrates and amino acids, inorganic nutrients such as nitrogen, phosphoric acid and potassium, and agar containing plant hormones such as auxin. Incubate in the medium. After a while, the cells of this tissue divide into cells to form callus, which is a mass of cells. The callus is separated from the medium and placed on a fresh solid medium. Subsequently, it is subcultured at regular intervals. The cultured callus is incubated in a liquid medium of the same composition except for agar only for mass production.

Various active ingredients in camphor callus provided by the present invention may be obtained using various extraction methods known in the art, and preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms. (Methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol with water, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol Can be obtained as an extraction solvent.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also herbal extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the present invention.

Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

Camphor ginseng extract according to the present invention has a whitening effect of reducing melanin production by melanogenesis cells (Table 1), inhibiting tyrosinase activity (Table 2).

In addition, it has an antioxidant effect (Table 3) that eliminates free radicals, and has low cytotoxicity (Table 4). Therefore, camphor ginseng extract of the present invention can be effectively used as a whitening composition that inhibits excessive melanin production. In addition, it can be usefully used as an antioxidant composition.

In the composition of the present invention, the camphor ginseng callus extract may be contained 0.005 to 50% by weight based on the total weight of the composition.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the camphor extract.

Cosmetics prepared with a cosmetic composition containing camphor ginseng callus extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition, cosmetics containing camphor ginseng callus extract of the present invention can be prepared in the form of a topical or systemic application commonly used in the field of dermatology by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to camphor ginseng callus extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, cleansing agents, face washes, soaps, treatments, Essences and the like.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Camphor Ginseng Extract for Skin Whitening

The camphor root was sterilized and cut to about 1 cm in length. 2,4-D (2,4-dichlorphenoxy acidic acid) at 0.5mg / L and 0.1mg / L Kinetin were added to MS medium (Sigma). After about two weeks, when amorphous callus tissues were induced, they were separated separately and placed on fresh solid medium and passaged at regular intervals. Thereafter, in a liquid medium of the same composition except for agar, incubated for 25 days at 25 ℃, mass production.

Test Example 1 Intracellular Melanin Inhibitory Effect

B16F1 melanoma cells were provided by the Korea Cell Line Bank and cultured. B16F1 melanoma cells are cell lines producing melanin and are generally used for whitening screening.

The medium for culturing the cells was sterile by adding DMEM, 10% FBS, 1% penicillin / streptomycin. Cell cultures were incubated in an incubator at 37 ° C., 5% CO ₂ . Melanoma cells were cultured and passaged when the 100 mm Petri dishes were about 90% full.

In order to measure the whitening effect of camphor callus extract, the amount of melanin produced by treatment with B16F1 melanoma cells was measured. Cells were seeded on 6-well plates and treated with Camphor ginseng extract prepared in Preparation Example 1 at 200 µg / ml, followed by incubation for 2 days, to recover the cells. The recovered cells were added with 1N NaOH containing 10% DMSO, reacted at 90 ° C for 1 hour to be sufficiently dissolved in melanin, and then absorbance was measured at a wavelength of 405 nm using an ELISA reader.

Experimental results were calculated by using the following equation 1 to inhibit the inhibition, shown in Table 1. Arbutin, which is known as a general whitening substance, has a melanin content of 72.6% at 200 ㎍ / ml, whereas camphor ginseng callus extract is 52.1%, which inhibits melanin production by 1.75 times higher than arbutin. It could be confirmed that.

Melanin Formation (%) = Treatment Absorbance / Untreated Absorbance × 100

Sample Name Untreated Arbutin Camphor callus extract Melanin Production (%) 100 72.6 52.1

Test Example 2 Measurement of Tyrosinase Activity Inhibition

It was determined whether the camphor extract affects tyrosinase enzyme activity, which plays an important role in producing melanin.

210 μl of 0.1 M phosphate buffer (pH6.8) is added to the 96 well plate, followed by 20 μl of mushroom-derived tyrosinase. After adding 40ul of 5mM L-DOPA as a substrate and reacting at 37'C for 10 minutes, the absorbance was measured at a wavelength of 475nm using an ELISA reader.

Tyrosinase activity was calculated using Equation 2 below. Table 2 shows the melanin inhibitory effects of kojic acid and the extract, which were widely used as whitening agents. When Koji acid was treated, only 2.5% of the total activity was active, whereas when treated with Camphor ginseng extract showed about 42.4%. Although the inhibitory effect was less than that of kojic acid, camphor ginseng callus extract was found to inhibit more than 57.6% of tyrosinase enzyme activity compared to the untreated group.

Tyrosinase Activity (%) = Treated Absorbance / Untreated Treated Absorbance × 100

Sample Name Untreated Kojisan Camphor callus extract Tyrosinase Activity (%) 100 2.5 42.4

Test Example 3 Toxicity Test

In order to use as a whitening cosmetic of Camphor ginseng callus extract, an experiment was carried out to check for cytotoxicity. Cells were inoculated into 96 well plates, treated with 200 ㎍ / ml of camphor ginseng extract, and after 2 days, the medium was removed, the reaction was carried out at 30'C for 4 hours by the addition of 100ul of MTT solution, and the stained cells were added with 100ul of DMSO. After melting, the absorbance was measured at a wavelength of 540 nm using an ELISA reader.

Cell viability was calculated using the following Equation 3, the results are shown in Table 3. Compared with the cells treated with camphor ginseng extract, it can be seen that despite the high concentration of 200 μg / ml, the viable cell rate was 98.8%, showing little cytotoxicity.

Cell viability (%) = (1-treated absorbance / untreated absorbance) × 100

Sample Name Untreated Arbutin Camphor callus extract Cell survival rate (%) 100 98.3 98.8

Test Example 4 Antioxidant Activity

In order to check the antioxidant activity of Camphor ginseng callus extract was measured using a DPPH solution which is a method of measuring the antioxidant activity.

200 μg / ml of Campus ginseng callus extract and DPPH solution, which is an antioxidant measurement solution, were mixed and reacted for 30 minutes at 37 ° C., and then absorbance was measured at 517 nm using an ELISA reader.

Experiments with vitamin C, known as antioxidants, compared the relative activity of extracts. As a result of the experiment, the antioxidant activity of camphor ginseng callus extract as shown in Table 4 was found to have a strong antioxidant power 86.5%. It is unusual that camphor ginseng callus extract shows better antioxidant activity than vitamin C.

% Antioxidant activity = (1-treated absorbance / untreated absorbance) × 100

Sample Name Vitamin c Camphor callus extract Antioxidant activity (%) 66.2% 86.5%

Examples of formulations for the composition of the present invention are illustrated below.

Preparation Example 2 Examples and Comparative Examples

To prepare a cosmetic composition containing the camphor ginseng extract of Preparation Example 1, and to compare with the cosmetic composition lacking the camphor ginseng extract was prepared in the composition ratio of Table 5.

division Content (% by weight) ingredient Example Comparative example Camphor callus extract 4.0 - Cetearyl Octanoate 5.0 5.0 Isopropyl Palmitate 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 Polysorbate 60 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 Spices, preservatives dose dose Purified water Balance Balance Sum 100 100

[Test Example 5]

In order to test the clinical whitening efficacy of the cosmetics of the present invention, 40 healthy adult men and women with blemishes, freckles, and pigmentation were randomly divided into two groups (A and B) of 20 people, In Example B, a comparative example was applied twice a day for 12 weeks for group B, and then the change in skin color was measured using chromatameter (Minolta CR300) to measure the change in brightness (ΔL) of color. The whitening effect was measured by objective visual observation by an expert and subjective visual observation by a subject. The results are shown in Table 6 below. At this time, the degree of whitening efficacy was classified into the following seven ratings.

* Evaluation criteria for whitening efficacy:

-3: worse, -2: worse, -1: slightly worse, 0: no change

, 1: slightly improved, 2: improved, 3: greatly improved

Subject Change in brightness of skin color (△ L) Objective evaluation of the skilled person Subjective Evaluation of Subject A group B group A group B group A group B group One 4.7 2.3 One 0 One One 2 5.0 2.9 2 0 2 0 3 4.6 3.2 3 One 3 One 4 4.4 2.3 One 0 One One 5 4.9 2.1 2 0 One 0 6 4.6 3.4 3 One 2 One 7 4.7 0.7 One 0 2 One 8 4.2 0.7 3 One One 2 9 4.8 2.5 One One 2 One 10 4.2 2.6 2 0 0 0 11 3.9 3.5 One 0 0 -One 12 3.7 3.8 3 0 One 0 13 4.5 2.4 2 0 2 One 14 4.4 1.5 0 0 One 0 15 4.3 2.2 3 One 3 One 16 4.7 2.9 2 One 0 0 17 5.6 2.4 One 0 One 0 18 5.0 3.0 One 0 0 One 19 5.2 2.9 0 0 0 0 20 5.1 2.7 3 2 One 0 Average 4.6 2.5 1.8 0.4 1.2 0.5

According to the results shown in Table 6, the change in skin brightness was 1.84 times more effective in applying the Examples than in the Comparative Examples, and the objective evaluation of the skilled workers and the subjective evaluation of the subjects were also compared with those of the Examples. The improvement was 4.5 times and 2.4 times, respectively.

As a result, it can be seen from the results shown in Table 6 that the cosmetics containing camphor ginseng callus extract prepared by the extraction example 1 according to the present invention can be seen that the excellent effect as to enhance the skin whitening effect have.

Formulation Example 1 Flexible Lotion

Based on the results of the preliminary experimental example, to present a cosmetic composition containing camphor ginseng callus extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples. Formulation example of the flexible lotion containing camphor ginseng callus extract was prepared as shown in Table 7.

Raw material Content (% by weight) Camphor callus extract 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Balance

Formulation Example 2 Nutrition Cream

Formulation of nutrition cream containing camphor ginseng callus extract was prepared as shown in Table 8.

Raw material Content (% by weight) Camphor callus extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Balance

Claims (4)

A cosmetic composition for skin whitening containing Panax Ginseng callus extract. Cosmetic composition for antioxidant containing camphor ginseng callus extract. The callus of claim 1 or 2, wherein the callus is Derived from one medium selected from MS medium, WHITE medium, B5 medium, R2 medium, The medium includes a growth regulator, The growth regulators are IAA (3-indoleacetic acid), IAld (3-indoleacetaldehyde), IPA (3-indolepyruvic acid), IAN (3-indoleacetonitrile), IAE (ethyl ester of indoleacetic acid), kinetin (Kinetin), BA ( benzoic acid), or CPPU (N- (2-chloro-4-pyridyl) -N'-phenylurea) is a cosmetic composition, characterized in that formed in one. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, Take one of the formulations selected from the group consisting of hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow Characterized in that the composition.