KR100894714B1 - Extract of humata tyermanni moore having skin whitening and anti-oxidative activation - Google Patents

Abstract

An extract of Humata Tyermanni Moore having a skin whitening effect is provided to produce a cosmetic composition for securing anti-oxidative activities and inhibiting melanogenesis in a melamine generation cell without cytotoxin. A cosmetic composition comprises Humata Tyermanni Moore extracts obtained by water, alcohol or a mixture thereof. The alcohol is a C1-C6 low alcohol. A method for preparing the Humata Tyermanni Moore extracts consists of the following steps of: dipping dried Humata Tyermanni Moore into water, alcohol or a mixture thereof to obtain an extract; cooling the obtained extract; filtering the cooled extract; evaporating the low alcohol from the filtered extract; and concentrating the extract.

Description

Extract of Humata tyermanni Moore having skin whitening and anti-oxidative activation}

The present invention is a white wool ( Humata) having skin whitening and sulfated activity tyermanni Moore ).

The most important pigment that determines human skin color is melanin, which is produced in the melanosomes of melanocytes in the basal layer of the skin. The complex biosynthesis process of melanin begins with tyrosine, an amino acid. Tyrosine is converted to dopaquinone by an enzyme called tyrosinase. Is generated. Melanosomes filled with melanin are transported to surrounding keratinocytes and lost through keratinization. However, when all this happens, overproduction or deposition of melanin pigment causes skin diseases such as blackening of the skin and frequent freckles and freckles.

There are several approaches to inhibiting melanin production. For example, inhibition of tyrosinase production, inhibition of tyrosinase activity, inhibition of reduction and photooxidation of existing melanin, or promotion of melanin excretion, and the like. As inhibitors of tyrosinase activity, kojic acid, arbutin, and derivatives thereof have been developed, but due to poor stability in the prescription system, they are degraded due to poor coloration, odor occurrence, efficacy at the biological level, uncertainty and safety of the effect. The use is limited due to problems. Kojic acid inhibits enzymatic activity by adsorbing copper ions present in the active site of tyrosinase, but it has been discontinued as a cosmetic ingredient because it is found to cause instability, skin side effects, and liver cancer in recent animal experiments. In addition, arbutin is a derivative in which glucopyranoside is combined with hydroquinone, and has little side effects when using hydroquinone and inhibits the synthesis of melanin pigments without toxicity to humans, thereby increasing melanin pigmentation. Applicability has been suggested as a therapeutic agent for the skin disease, which has the disadvantage of being partially degraded by skin enzymes. Substances that are active at the same time to remove free radicals and have antioxidant and whitening effects have been in the spotlight recently, including coenzyme, vitamin E and green tea extract.

In the search for new raw materials, many researches on natural products are being conducted because of excellent safety and various active ingredients. Examples of the whitening cosmetics containing natural products include Sangbaekpi (Japanese Patent Laid-Open No. 55-44375, No. 64-26507, No. 64-83009, No. 1-25687, No. 5-139950 and No. 92). -002109, 97-021273), licorice (Japanese Patent Laid-Open No. 60-214721, Japanese Laid-Open No. 60-214728, Japanese Laid-Open No. 63-23809, Korean Patent No. 64-63506, Hei 1-149706, and Korean Laid-Open Patent Publication 92-002109, 97-025601), cinnamon (Japanese Patent Laid-Open No. 63-30403, Hei 5-139954), Gosam (Japanese Patent Laid-Open No. 64-26507 and Korean Patent Laid-Open No. 92-002110) Aloe (Japanese Patent Laid-Open No. 52-44375, Hei 2-207030, Hei 5-139950) and the like are typical. However, it has been found that many of these plant extracts and herbal extracts act on tyrosinase to inhibit melanin production, but they also have many problems in using them beyond the effective concentration in cosmetics and pharmaceuticals in terms of safety and discoloration. It does not have an excellent effect yet.

^- superoxide (superoxide, O ₂₎ by a variety of oxidation reactions, chemicals, food, human diseases and radiation Cell membranes containing large amounts of unsaturated fatty acids are generated when highly reactive free radicals such as hydroxyl radicals (OH) and hydrogen peroxide (H ₂ O ₂ ) are produced in vivo. Lipid peroxide is oxidized to produce lipid peroxides in the cell membrane. Accumulation of lipid peroxides on the cell membrane reduces the fluidity and functionality of the cell membrane, thereby inhibiting the overall function of the cell and changing the structure of the cell. In addition, free radicals and free radicals modify or destroy cell constituents such as nucleic acids and sugars, resulting in cancer, liver disease such as alcoholic hepatitis, stroke due to cerebrovascular disorders, myocardial infarction, diabetic vascular disorders, hyperlipidemia, and acute inflammation. And various human diseases such as rheumatic diseases. In addition, reactive oxygen reacts with unsaturated fatty acids, which are present in a large amount in foods, which causes rancidity, which may result in deficient defects in food safety and quality maintenance, and oxidative stress of plants and animals caused by various oxides, and active oxygen generated during fermentation of microorganisms. In many fields, such as yield reduction caused by free radicals are causing problems.

With the theory that aging and geriatric diseases are caused by reactive oxygen species, research on the development of antioxidants known as substances capable of regulating reactive oxygen species has been actively conducted, leading to superoxide dismutase and peroxidase. antioxidants such as peroxidase, catalase and glutathione peroxidase, and low molecular weight antioxidants derived from natural products such as tocopherol, ascorbate, carotenoid and glutathione Much research has been done (Chang, SS, et al., J. Food Sic. 42: 1102, 1977; Hammerschmidt, PA and Pratt, DE, J. Food Sic . 43: 556, 1964), 2,6 Synthetic antioxidants such as di-tert-butyl-4-hydroxyanisol (2,6-di-tert-butyl-4-hydroxyanisole, BHA) have been developed and used in medicine and food fields (Kitahara, K , et al., Chem Pharm . 40: 2208, 1992; Hatano, T., Natural Medicines 49: 357, 1995; Masaki, H., et al., Biol. Pharm. Bull. 18: 162, 1995).

However, consumer avoidance of synthetic antioxidants and carcinogenicity have been reported in animal experiments in which large amounts of synthetic antioxidants have been administered (Branen, AL, JAOCS 52:59, 1975). The use of synthetic antioxidants is increasingly limited. As such, there is an urgent need to develop new natural antioxidants that are both effective and safer.

On the other hand, aunt living in the by-product as a herbal davalliaceae (Davalliaceae) and the scientific name Humata tyermanni It is Mooore . The height is about 20cm, the rhizome is long and stretches to the side. It grows on the branches or rocks of old trees and is mainly distributed in eastern, southern and southwestern China. It is used as a medicine after drying the rhizome. The shape is flat and long, curved like a snake. The length is 15 ~ 30 cm and the diameter is about 0.5 cm. It removes the blood, smooths the blood, removes the custom, and has a diuretic effect.

Therefore, the present inventors are studying natural medicinal plants for the whitening cosmetic composition which is safe to the skin and has a low possibility of discoloration , and the extract extracted from Humata tyermanni Moore , a plant growing in the high mountains of China, inhibits melanin production and antioxidant activity. Thus, the present invention has been completed by revealing that it can be usefully used as a cosmetic composition or antioxidant composition for skin whitening.

An object of the present invention is to provide a white wool extract ( Humata tyermanni Moore ) having skin whitening and antioxidant activity.

In order to achieve the above object, the present invention provides a white wool extract ( Humata tyermanni Moore ) having a whitening and antioxidant activity.

The present invention also provides a cosmetic composition for whitening containing the white wool extract.

The present invention also provides an antioxidant containing the white wool extract as an active ingredient.

The present invention also provides a pharmaceutical composition for the prevention and treatment of diseases caused by the accumulation of excess active oxygen containing the white wool extract as an active ingredient.

The present invention also provides an anti-aging pharmaceutical composition containing the white wool extract as an active ingredient.

In addition, the present invention provides a cosmetic for preventing skin aging containing the white wool extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

White wool extract according to the present invention can be prepared as follows.

Wash the white wool with water and dry it in the shade. The dry white wool is placed in an extraction vessel and extracted with water, alcohol or a mixture thereof for a certain time at a suitable temperature. The extract can be filtered and concentrated.

As the extraction solvent, it is preferable to use a solvent selected from water, C ₁ to C ₃ lower alcohols, or a mixed solvent thereof, and more preferably to use methanol.

The amount of the extraction solvent is 2 to 10 times, preferably 4 times, the dry weight of the herbal medicine.

The extraction method may be an extraction method such as heat extraction, reflux cooling extraction and ultrasonic extraction, preferably, may be extracted once to 5 times as the extraction method of the heat extraction.

The deposition temperature is 30 ° C to 70 ° C, more preferably 40 ° C to 60 ° C, and most preferably maintained at about 40 ° C is most effective for the prevention of rot, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After obtaining the white wool extract, it is possible to remove the solids using a filter paper, the suspension is centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract is concentrated by removing methanol in the extract using nitrogen.

White hair extract according to the present invention inhibits melanin production superior to arbutin known as a general whitening material in B16F10 melanoma cells (see FIG. 1 and Table 1) and has a whitening effect. In addition, B16F10 melanoma cells were treated at a high concentration of 200 μg / ml and showed no cytotoxicity (see FIGS. 2 and 2) and showed antioxidant activity of scavenging free radicals (see FIGS. 3 and 3). Antioxidant activity is not superior to vitamin C, even if extracted at room temperature for a long time when extracted, it can be seen that the effect is stable.

The present invention also provides a cosmetic composition for skin whitening containing white hair extract as an active ingredient.

White hair extract of the present invention can be effectively used as a cosmetic composition for whitening to suppress the excessive melanin production as described above. In addition, it can be effectively used as an antioxidant cosmetic composition.

In the composition of the present invention, the white wool extract is preferably contained in an amount of 0.005 to 50 (% by weight) based on the total weight of the composition, but may be used in the range above or below the range according to the use purpose in the range of whitening effect and no toxicity. Can be.

The composition of the present invention may further contain one or more active ingredients exhibiting the same or similar functions in addition to the white wool extract.

Cosmetics prepared with a cosmetic composition containing the white wool extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the white wool extract of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be applied topically or systemically applied commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the white wool extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic or dermatological arts. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

The present invention also provides an antioxidant containing the white wool extract as an active ingredient.

Although the white wool extract of the present invention does not have an excellent antioxidant activity than vitamin C (see FIG. 3 and Table 3), it has a high stability and can effectively remove harmful radicals in vivo because the antioxidant activity is maintained over a long time.

The present invention also provides a pharmaceutical composition for the prevention and treatment of diseases caused by the accumulation of excess active oxygen containing the white wool extract as an active ingredient.

The present invention also provides an anti-aging pharmaceutical composition containing the white wool extract as an active ingredient.

Baekmosa extract of the present invention can be usefully used as a pharmaceutical composition for the prevention and treatment of diseases caused by the excessive accumulation of effective free radicals due to the antioxidant activity or a pharmaceutical composition for preventing aging.

The composition of the present invention may further include conventionally used excipients, disintegrants, sweeteners, lubricants, flavoring agents, etc., tablets, capsules, powders, granules, suspensions, emulsions, syrups, It may be formulated in other liquids.

Specifically, the compositions of the present invention may be formulated for oral administration, for example, stagnation, troches, lozenges, aqueous or dominant suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs Formulated as elixirs. Formulations such as tablets and capsules for lactose, saccharose, sorbitol, mannitol, starch, amylopectin, binders such as cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, stearic acid Lubricants such as magnesium, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. Capsules contain liquid carriers, such as fatty oils, in addition to the substances mentioned above.

In addition, the composition of the present invention can be administered orally or parenterally, it is preferable to select subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection injection method when parenteral administration. To formulate into parenteral dosage forms, the white wool extract of the present invention is mixed in water with a stabilizer or buffer to prepare a suspension, which is formulated in unit dosage forms of ampoules or vials.

The dosage of the active ingredient according to the present invention is appropriately selected according to the absorption rate, inactivation rate and excretion rate of the active ingredient in the body, the age, sex and condition of the patient, the severity of the disease to be treated, but in the case of oral administration in general Adults can be administered once to several times divided by the amount of 0.1 ~ 0.2 g of white wool extract of the present invention per kg of body weight per day, it is preferable to administer in an amount of 0.1 to 10 mg.

The composition of the present invention can be used alone or in combination with methods using surgery, hormonal therapy, drug treatment and biological response modifiers for the prevention of aging as well as the prevention and treatment of diseases caused by excessive accumulation of free radicals. have.

In addition, the present invention provides a cosmetic for preventing skin aging comprising the extract of white wool.

The anti-aging cosmetics of the present invention include, but are not limited to, lotions, ointments, gels, creams, patches or sprays. In preparing the skin anti-aging cosmetics containing the white wool extract of the present invention, the white wool extract of the present invention may be added in an amount of 1 to 15 parts by weight, preferably 2 or 10 parts by weight, to the externally used skin composition. Can be. In addition to the white wool extract of the present invention, the skin external preparation of the present invention includes fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, and surfactants. Common to water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for the skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as. The ingredients may also be introduced in amounts generally used in the field of dermatology.

As described above, the white wool extract of the present invention is extracted using a solvent selected from water, a lower alcohol of C ₁ to C ₃ or a mixed solvent thereof, inhibits melanin production by B16 melanoma cells, Since there is little toxicity and anti-oxidant activity, the cosmetic composition for skin whitening and active oxygen may be usefully used as an anti-aging cosmetic product as well as a pharmaceutical composition for preventing and treating diseases caused by excessive accumulation.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Formulation Examples.

However, the following Examples, Experimental Examples, and Formulation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples, Experimental Examples, and Formulation Examples.

Example 1 Preparation of White Wool Extract for Skin Whitening

The white wool ( Humata tyermanni Moore ) of the present example used what was collected from the high mountain region of Yunnan, China. 100 g of the collected sample was immersed in 1 L of 99.5% methanol and extracted by heating in a 40 ° C. water bath for 10 hours. The obtained extract was filtered with filter paper (4 watts only) before cooling. The filtered extract was concentrated using a rotary vacuum concentrator (EYELA) and the remaining methanol was removed with nitrogen gas to obtain about 10 g of white wool extract.

Experimental Example 1 Measurement of Whitening Effect

<1-1> B16F10 melanoma cell culture

B16F10 cells were grown in the American Type Culture Collection (ATCC) as a cell line producing melanin. The medium for culturing the cells was used by adding 10% FBS (fetal bovine serum) and 1% penicillin-streptomycin to Dulbecco's Modified Eagle's Medium (DMEM). Cells were cultured for 3 days in an incubator of 37 ° C., 5% CO ₂ and passaged when 100πdish was 90% full.

<1-2> Inhibition of melanin production using absorbance measurement

In order to examine the whitening effect, the melanin content produced by treating 200 μg / ml white wool extract to B16F10 cells was measured. Cells were seeded at a concentration of 0.3 × 10 ⁵ cells / ml in 6-well plates and cultured for 24 hours, and then treated with white wool extract or arbutin of Example 1, and the cells were recovered after 48 hours of culture. The recovered cells were added with 1N NaOH containing 10% DMSO (dimethylsulfoxide), and the cells were cut at 80 ° C. for 1 hour, and then absorbance was measured at 405 nm using an ELISA. Melanin production was calculated using the following equation 1, the experimental results are shown in Table 1 and FIG.

Melanin Formation (%) = Treatment Absorbance / No Treatment Absorbance × 100

Sample Name No treatment Arbutin White Wool Extract Melanin Production (%) 100 78.3 66.5

As shown in Table 1, in the case of arbutin known as a general whitening substance, the white wool extract showed 66.5%, compared to 78.3% of the melanin at 200 μg / ml, which shows that the melanin production inhibitory effect was excellent. .

Experimental Example 2 Toxicity Test

In order to determine whether the white wool extract can be used as a whitening cosmetic composition, a cytotoxicity experiment was conducted. Cells were inoculated in a 96 well plate at a concentration of 12.5 × 10 ³ cells / ml, treated with 200 μg / ml of white wool extract after 24 hours of incubation, and after 48 hours of incubation, MTT (3- (4,5-dimethythiazol-2- yl) -2,5-diphenyltetrazolium bromide) solution was treated with 100 μl / well. MTT solution was used to dissolve 5 mg MTT powder in 1 ml PBS (phosphate buffered saline) and diluted 10-fold with DMEM medium. Cells treated with MTT solution were continuously incubated for 4 hours, medium was removed, 100 μl / well of DMSO (dimethyl sulfoxide) was added, and the absorbance was measured at 540 nm by ELISA. Cell viability was calculated using the following equation 2, the results are shown in Figure 2 and Table 2.

Cell survival rate (%) = (treatment absorbance / no treatment) × 100

Sample Name No treatment Arbutin White Wool Extract Cell survival rate (%) 100 109.3 107.8

As shown in Table 2, when the non-treated group at 100%, the white wool extract, despite the high concentration of 200 μg / ml, the cell survival rate is 107.8%, it can be seen that there is no cytotoxicity as the number of cells increases rather.

Experimental Example 3 Antioxidant Activity

DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was used to confirm the antioxidant activity of the white wool extract. Dilute DPPH dissolved in 1.2 mg / ml of ethanol in 96-well plate 10 times, add 100 μl to each well, add 200 μg / ml of white wool extract, react for 30 minutes in a 37 ° C incubator, and measure absorbance at 517 nm with ELISA. It was. The control was unsampled and vitamin C was used (Si Eun Lee, Eun Mi Ju, Jee Hee kim. 2001. free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root.Exp Mol Med. 33 (4): 263-8). Antioxidant activity was calculated using the following equation (3), the experimental results are shown in Figure 3 and Table 3.

Antioxidant Activity (%) = (1-treated Absorbance / Untreated Absorbance) × 100

Sample Name vitamin White Wool Extract Antioxidant activity (%) 60.8 31.1

As shown in Table 3, white wool extract (31.1% at 200 μg / ml) showed antioxidant activity.

Examples of preparations for the compositions of the present invention are illustrated below.

Preparation Example 1 Preparation of a Whitening Cosmetic Containing White Wool Extract as an Active Ingredient

<1-1> preparation of flexible lotion

Formulation example of the flexible Hwajeongsu containing white wool extract as an active ingredient was prepared as shown in Table 4.

Raw material Content (parts by weight) White Wool Extract 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

<1-2> Preparation of nourishing cream

Formulation example of nutrition cream containing white wool extract was prepared as shown in Table 5.

Raw material Content (parts by weight) White Wool Extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

<Example 2> Preparation of an aging inhibitor containing white wool extract as an active ingredient

<2-1> Preparation of Syrup

Syrup containing white wool extract as an active ingredient was prepared as shown in Table 6.

Ingredient Content (parts by weight) White Wool Extract 2 saccharin 0.8 Party 25.4 glycerin 8 Spice 0.04 ethanol 4 Sorbic acid 0.4 Distilled water 60

<2-2> Preparation of Tablet

Tablets containing white wool extract as an active ingredient were prepared as shown in Table 7.

Ingredient Content (parts by weight) White Wool Extract 250 Lactose 175.9 Potato starch 180 Colonde silicic acid 32 10% gelatin solution 5 Potato starch 160 talc 50 Magnesium stearate 5

250 parts by weight of white wool extract, 175.9 parts by weight of lactose, 180 parts by weight of potato starch and 32 parts by weight of colloidal silicic acid were mixed. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. This was dried and the mixture obtained by adding 160 parts by weight of potato starch, 50 parts by weight of talc and 5 parts by weight of magnesium stearate was prepared as a tablet.

1 is a graph showing the melanin production when white wool extract treatment,

Figure 2 is a graph showing the cell viability when treated with white wool extract,

3 is a graph showing the antioxidant activity of the white wool extract.

Claims (9)

Extracted with water, alcohol or mixtures thereof, Humata has skin whitening activity tyermanni Moore ) cosmetic composition for skin whitening comprising the extract as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the alcohol is a C ₁ to C ₃ lower alcohol. The method of claim 1, wherein the white wool extract 1) warming the dried white wool sample by immersing it in water, alcohol or a mixture thereof; 2) cooling the extract obtained through step 1); 3) filtering the cooled extract of step 2); 4) evaporating the lower alcohol from the filtrate of step 3); And 5) A cosmetic composition for skin whitening, characterized in that it is extracted by concentrating the obtained product of step 4). The cosmetic composition for skin whitening according to claim 1, wherein the skin whitening activity is due to inhibition of melanin production of cells. An antioxidant which is extracted with water, alcohol or a mixture thereof and contains white wool extract having antioxidant activity as an active ingredient. A pharmaceutical composition for skin whitening extracted with water, alcohol or a mixture thereof and comprising Humata tyermanni Moore extract as an active ingredient having skin whitening activity. delete delete Cosmetics for preventing skin aging, extracted with water, alcohol or a mixture thereof, containing white wool extract having antioxidant activity as an active ingredient.

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