KR100531686B1 - Cosmetic composition comprising the extract of Machilus Thunbergii for skin whitening - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition containing an extract of Tohubak, and more particularly to a whitening cosmetic composition containing a crude extract or a non-polar solvent-soluble extract of Tofubak, which has an excellent activity of inhibiting melanin synthesis. It is about.

Description

Cosmetic composition comprising the extract of Machubac {Cosmetic composition comprising the extract of Machilus Thunbergii for skin whitening}

The present invention relates to a cosmetic composition comprising a herbal extract, and more particularly, to a cosmetic composition for whitening containing Machilus Thunbergii extract having a melanin synthesis inhibitory activity.

Hyperpigmentation of the skin can be caused by a number of factors, such as hormonal abnormalities in the body after the inflammatory response of the skin, hereditary diseases, and ultraviolet irradiation, mainly due to melanin pigment synthesis abnormalities and distribution abnormalities.

The main function of melanin is to remove oxygen radicals and protect the skin from the damage thereby. Therefore, high levels of melanin mean that it has an effective response system to protect the skin from physical and chemical toxic substances. The production of melanin is converted to dopaquinone by tyrosine (Tyrosinase) action in the melanocytes (Tyrosinase), and then by the action of the enzyme and spontaneous oxidation reaction. Therefore, the method of suppressing melanin production to prevent the skin from blacking can be largely divided as follows.

Firstly, by blocking the ultraviolet rays to remove the main cause of melanin production, this method allows the cosmetic composition to contain a light scattering agent or light blocking agent can be expected good results.

Second, it is possible to inhibit melanin production by inhibiting the synthesis of core carbohydrates necessary for tyrosinase such as glucosamine to be active.

Third, there is a method of interfering with the function of tyrosinase, an enzyme involved in melanogenesis, such as kojic acid or arbutin.

Fourth, there is also a method that interferes with cell division because it has specific toxicity to melanocytes, which produce melanin, such as hydroquinone. In addition, there is a method of reducing the produced melanin to decolorize.

In order to suppress the melanin production process, ascorbic acid (ascorbic acid), kojic acid, arbutin, hydroquinone (hydroquinone) and natural plant extracts have been used as a whitening material. However, Koji acid or arbutin, which is a representative whitening material, has a problem of low product safety despite strong whitening effects.

Recently, as whitening raw materials using natural plant extracts, which have been used for a long time as folk remedies, have been recognized as safe for humans, they have been spotlighted as raw materials for whitening coatings.

Therefore, there is a need for research on whitening raw materials using extracts of natural plants that are excellent in whitening and are safe for humans.

The tofu foil used in the present invention is Machilus thunbergii SIEB. Et Z., and the bark is called Machili Cortex. The plant contains lignans, lignan glycosides, alkaloids, flavonoids and essential oils, in particular the bark contains 0.48% tannins, 12.38% resins, 0.688% caoutchouc and large amounts of mucus. Roots benzyl isoquinoline (benzyl isoquinoline) of the n-based alkaloids-are contained Nord Armero pabin (n -norarmepavine) and retinoic kyulrin (reticulin). The mucus contained in the leaves is a complex of polysaccharides and proteins, and the composition of the viscous polysaccharides consists of arabinose, xylose, rhamnose, galactose, glucose and glucose. Glucuronic acid. Leaves and twigs contain myristic acid and have been used for leg edema, abdominal pain and abdominal swelling. Stomach weakness, posterior stomach, indigestion, fever, high humidity (복), abdominal and medicinal effects.

Korean Patent Registration No. 207958 discloses Licarin, which specifically inhibits the activity of Acyl-CoA: Cholesterol Acyltransferase (ACAT) from Machilus thunbergii Sieb et Zucc. The present invention relates to a method for preparing a compound and a composition for preventing and treating cardiovascular diseases caused by hypercholesterolemia containing the same, and Korean Patent Registration No. 78252 relates to a skin cosmetic containing a gall bladder, a barley or a thick leaf extract. The present invention relates to a skin cosmetic having a function of preventing damage to skin cells from blood and preventing and protecting against aging.

 However, none of the above documents discloses that Tofubac has melanin synthesis inhibitory activity.

In order to search for substances inhibiting skin pigmentation, the present inventors studied various plant extracts, and showed that the Tobacco nonpolar solvent soluble extract exhibited excellent melanin synthesis inhibitory activity, so that the whitening effect was excellent. The invention has been completed.

An object of the present invention is to provide a cosmetic composition for whitening containing Tobacco extract having melanin synthesis inhibitory activity.

In order to achieve the above object, the present invention provides a cosmetic composition for whitening containing the crude extract of Machilus Thunbergii .

The crude extract includes water, methanol, ethanol and the like, or a lower alcohol or a mixed solvent thereof, preferably an extract available in 70% methanol.

The present invention also provides a cosmetic composition for whitening containing a non-polar solvent soluble extract of Tofu foil.

The nonpolar solvent soluble extract includes extracts soluble in nonpolar solvents, preferably methylene chloride, such as hexane, carbon tetrachloride, chloroform, methylene chloride, ether or ethyl acetate.

The present invention also provides a cosmetic composition for whitening containing a polar solvent soluble extract of Tofu foil.

The polar solvent soluble extract of Tofu Bak is obtained by removing the non-polar solvent soluble part from the crude extract, a polar solvent such as lower alcohol such as methanol, ethanol, or a mixed solvent thereof, and a mixed solvent thereof, preferably extractable in water. It includes.

Hereinafter, the present invention will be described in detail.

Tofubak extract of the present invention can be obtained by the following method.

Specifically, about 1 to 20 times, preferably about 2 to 5 times, water, lower alcohols such as methanol or ethanol, or mixtures thereof, of the tobacco weight (kg) sufficiently dried, shredded or ground in the shade. 1 to 10 times, preferably for about 1 to 2 days, preferably about 2 to 1 day, at 20-100 ° C., preferably 50-100 ° C. extraction temperature with a solvent, preferably 70% methanol 2 to 5 times repeatedly to obtain a crude extract, a soluble extract according to water, a lower alcohol or a mixed solvent thereof, by filtration method such as hot water extraction, ultrasonic extraction, reflux cooling extraction, and concentrated under reduced pressure or heating The extract concentrate can be obtained, and can be additionally lyophilized to obtain a powder form.

Then, the crude extract is suspended in water, and then the suspension is added once by adding a nonpolar solvent such as hexane, carbon tetrachloride, chloroform, methylene chloride or ethyl acetate in a volume of about 1 to 100 times, preferably about 1 to 5 times. By fractionation to 10 times, preferably 2 to 5 times, the nonpolar solvent soluble extract and the polar solvent soluble extract of the present invention can be obtained. It is also possible to further carry out conventional fractionation processes (Harborne JB Phytochemical methods: A guide to modern techniques of plant analysis. 3rd Ed. Pp 6-7, 1998).

For example, once extracted with 70% methanol of 1 to 3 times the weight of the dried Tofu foil at about 50 to 70 ° C. under reflux cooling for 10 to 18 hours, and then extracted twice with 90% methanol under the same conditions. The total extract was concentrated under reduced pressure to obtain a crude ethanol extract, and distilled water and methylene chloride were added to the crude extract, and the distilled water layer and the methylene chloride layer were fractionated three times using a fractional funnel. Concentrated under reduced pressure, polar solvent soluble extract and non-polar solvent soluble extract can be obtained.

In addition, the purified fraction of the active ingredient of the present invention can be separated by fractionating and concentrating the non-polar solvent soluble extract, using silica gel column chromatography and thin layer column chromatography (TLC).

The present invention provides a method for preparing an extract having melanin synthesis inhibitory activity from Tohubak.

The methanol extract and methylene chloride fraction of Tohubak have excellent action of inhibiting melanin pigment synthesis.

The present invention provides a cosmetic composition for whitening comprising the Tofu thin crude extract or the non-polar solvent soluble extract obtained by the above production method as an active ingredient.

The content of crude extract or nonpolar solvent soluble extract of Tofu foil added to the cosmetic composition is preferably 0.001 to 10% by weight of various cosmetics, more preferably 0.01 to 5% by weight.

The whitening cosmetics containing the Tohubak extract of the present invention has a variety of formulations such as softening longevity (skin), astringent longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, skin adhesive patch and skin adhesive gel, face wash It can be prepared as a cosmetic.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherolvitamin E nicotinate, DL-pantothenyl alcohol, D-pantotenyl Alcohols, pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, pit spingosine, sphingolipid lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats and vegetable fats and oils, and the like.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristin, myristyl acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, cesinooleic acid cetyl , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethyl hexateate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-stealoyl hydroxystearate stearyl, 12-stealoyl Ester system, such as hydroxystearic acid isostearyl, etc. are mentioned.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methyl steoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymers include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymer include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, and high molecular silicones.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE · POP (Polyoxyethylene Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzyl ammonium bromide, behenyltrimethylammonium chloride, and chlorides. Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, carbon black and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange; And composite pigments of these inorganic pigments and organic pigments.

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-paratoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate and cinnamic acid. Benzyl, paramethoxy cinnamic acid 2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urokanine Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hydroxy 5-methylphenyl) benzotriazole and the like.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% compounding.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

The cosmetic of the present invention is a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts in addition to Tohubak extract (and combination ingredients which may be added in addition to those exemplified above as necessary). And the like) can be obtained according to a known method, for example, according to the method described in the "Transdermal formulation development manual" Matsumoto Michio Supervision First Edition (Seishi-ken, 1985).

Tohubak extract of the present invention can be used with confidence because there is little toxicity and side effects.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the examples are only for illustrating the present invention and the present invention is not limited thereto.

Example 1 Preparation of Tobacco Crude Extracts

Tofubak bark purchased from a herbal medicine store in Daegu Yangnyeongsi was used after receiving taxonomic review from Professor Jeunghwan Hwan of Daejeon Chungnam National University. After drying, 10 kg of finely chopped tobacco was added to 20 l of 70% methanol, extracted once with reflux cooling at about 60 ° C. for 12 hours, and extracted twice with 20 L of 90% methanol under the same conditions. The filtrate was concentrated under reduced pressure at 65 ° C. to remove methanol, and dried to obtain 1.45 kg of Tobacco crude extract.

Example 2 Preparation Example I of a Tobacco Nonpolar Solvent Soluble Extract

1.45 kg of the Tobacco crude extract of Example 1 was suspended in 2 L of distilled water, and then, 2 L of methylene chloride was added and fractionated into a distilled water layer and a methylene chloride layer by a fraction funnel, and the distilled water layer and the methylene chloride layer were subjected to three times. Concentration under reduced pressure afforded 1102.0 g of distilled water extract and 193.86 g of methylene chloride extract.

Example 3 Preparation Example II of Tobacco Nonpolar Solvent Soluble Extracts

1.45 kg of Tobacco crude extract of Example 1 was suspended in 2 L of distilled water, 2 L of ethyl acetate was added thereto, and fractionated into a distilled water layer and an ethyl acetate layer by a fraction funnel, and the distilled water layer and the ethyl acetate layer were subjected to three times. Concentration under reduced pressure afforded 1102.0 g of distilled water extract and 390 g of ethyl acetate extract.

Experimental Example 1. Experiment of melanin synthesis inhibitory effect

Melanin synthesis inhibition assays were performed with minor modifications to the method of Curto (Curto E. et al . ; Biochem. Pharmacol. , 57 , pp663-672, 1999).

B-16 mouse melanoma cell line (2 × 10 ⁴ cells / 200 μL, sold by LG H & W) was incubated in 96-well plates using Eagle's minimum essential medium. Cells were cultured for one day at 37 ° C. and 5% CO ₂ -95% atmosphere, and then dissolved in dimethyl sulfoxide (DMSO). 2 Tofubak methylene chloride fraction (25 µg / ml) or distilled water fraction (25 µg / ml) and Tofubak ethyl acetate fraction of Example 3 (25 µg / ml) were added to the culture medium. Only DMSO was added to the control group, and kojic acid (150 µg / ml, Sigma) and arbutin (50 µg / ml, Sigma) were used as positive controls. After 2 more days of incubation, the attached cells were washed with 200 μl of PBS (pH 7.2), 200 μl of 1N NaOH was added, and the cells were homogenized. Absorbance was measured using an ELISA reader at 405 nm and inhibitory activity was calculated to varying degrees from the initial absorbance values.

Inhibitory Effects of Tohubak's Solvent Extracts on Melanin Synthesis Solvent extract % Inhibition Methanol extract 64.3 Methylene chloride fraction 42.9 ± 5.2 Ethyl acetate fraction 2.45 ± 4.2 Distilled water fraction 3.12 ± 4.7 ^a Kojisan 22.0 ± 3.8 Arbutin 28.3 ± 2.4 Each figure represents the mean ± standard deviation of the results of 4 experiments. A indicates a significant difference from the positive control group ( p <0.01).

Experimental Example 2. Cytotoxicity Test

Melanoma B-16 cell line (2 × 10 ⁴ cells / 200 μl) was incubated in 96-well plates using Eagle Minimal Media. The cells were incubated for one day at 37 ° C. and 5% CO ₂ -95% atmosphere, and then the crude extract extracted in DMSO was added to the culture medium. After culturing for another 2 days, cytotoxicity was observed through MTT color analysis using tetrazolium. Absorbance (OD) was measured using an ELISA reader at 540 nm.

As a result, the cytotoxicity of Tobacco crude extract was about 100 μg / ml LD50.

Hereinafter, a formulation example of the cosmetic composition will be described, but the present invention is only intended to be described in detail and not intended to limit it.

Flexible Cosmetic (Skin) Manufacturing

Tobacco extract of Example 2 ....... 1.00 (%)

Glycerol ............... 3.00

Ethanol ......................................... 5.00

Propylene Glycol ...................... 2.00

Incense ............................

The total amount was 100 with purified water, and mixed with the compounding ratio according to a conventional method to prepare a flexible cosmetic water.

Lotion manufacturers

Tobacco extract of Example 2 ....... 3.00 (%)

L-ascorbic acid-2-magnesium phosphate salt ...... 1.00

Water Soluble Collagen (1% Solution) ................. 1.00

Sodium Citrate ..... 0.10

Citric Acid ........................... 0.05

1,3-butylene glycol ............... 3.00

The whole amount was 100 with purified water, and a lotion was prepared at the above compounding ratio.

Cream manufacturers

Tobacco extract of Example 2 ....... 1.00 (%)

Polyethylene Glycol Monostearate ............... 2.00

Self-emulsifying glycerin monostearate ............ 5.00

Cetyl Alcohol ... 4.00

Squalene ............... 6.00

Glyceryl tri2-ethylhexanoate ......... 6.00

Sphingose Sugar Lipids ......................... 1.00

1.3-Butylene Glycol ... 7.00

The whole amount was 100 with purified water, and a cream was prepared at the above compounding ratio.

Pack manufacturer

Polyvinyl Alcohol ... 13.00 (%)

L-ascorbic acid-2-magnesium phosphate salt ...... 1.00

Tobacco extract of Example 2 ............. 5.00

Lauroylhydroxyproline ......... 1.00

Water Soluble Collagen (1% Aqueous Solution) ..... 2.00

1,3-butylene glycol ............... 3.00

Ethanol ......................................... 5.00

The whole amount was 100 with purified water, and a pack was prepared at the above compounding ratio.

Essence

Hydroxyethylene cellulose (2% aqueous solution) ...... 12.00 (%)

Xanthan gum (2% aqueous solution) ............ 2.00

Tobacco extract of Example 2 ............. 2.00

1,3-butylene glycol ......... 6.00

Dark glycerin ... 4.00

Sodium hyaluronate (1% aqueous solution) ................... 5.00

The whole amount was 100 with purified water, and a cosmetic liquid was prepared at the blending ratio (%).

The cosmetic composition using the Tobacco crude extract and the non-polar solvent soluble extract provided by the present invention have superior melanin synthesis inhibitory effect than kojic acid, and are safe as herbal extracts, and are used as creams, skins, lotions, and packs for whitening cosmetics. Can be.

Claims (5)

delete delete A cosmetic composition for whitening containing methylene chloride soluble extract of Tofubak. delete delete