KR102222360B1 - Cosmetic composition for anti-inflammation or improving skin trouble containing herb extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving anti-inflammatory or trouble comprising an herbal extract, and more particularly, anti-inflammatory comprising one or more extracts or fractions thereof selected from the group consisting of ginseng, licorice, rhizome and bokryeong as an active ingredient. Or it relates to a cosmetic composition for improving trouble.

Description

Cosmetic composition for anti-inflammation or improving skin trouble containing herb extracts}

The present invention relates to a cosmetic composition for improving anti-inflammatory or trouble comprising an herbal extract, and more particularly, anti-inflammatory comprising one or more extracts or fractions thereof selected from the group consisting of ginseng, licorice, rhizome and bokryeong as an active ingredient. Or it relates to a cosmetic composition for improving trouble.

Inflammation is the body's immune response in response to wounds or diseases, and oxidative stress such as ultraviolet rays, free radicals, etc. activates inflammatory factors, causing various diseases and aging of the skin. Vasoactive polypeptides such as kinin, plasmin, and complement act as vasodilation, contraction and chemotaxis, and other lymphokines such as interleukin-6 (IL-6). Phosphorus and arachidonic acid are responsible for the inflammatory response. Arachidonic acid is metabolized to prostaglandin and leukotriene, which are inflammatory mediators through two pathways of cyclooxygenase or lipooxygenase, mediating various inflammatory reactions.

On the other hand, in order to eliminate inflammation, it is called an anti-inflammatory agent that acts to remove inflammatory sources, reduce biological reactions, and symptoms. Substances used for anti-inflammatory purposes to date include flufenamic acid, ibuprofen, benzydamine, and indomethacin as a nonsteroidal type, and prednisolone as a steroid type. , Dexamethasone and the like. In addition, allantoin, azene, hydrocortisone, etc. are known to have anti-inflammatory effects, but these substances are limited in use due to safety issues on the skin, or the effect is insignificant, so the effect of substantially reducing inflammation cannot be expected. There is this.

Under this background, the present inventors have conducted research on substances that are non-toxic and excellent in skin condition improvement effects from natural resources, and thus at least one herbal medicine selected from the group consisting of ginseng, lichen, rhizome, and bokryeong It was found that the composition containing the extract as an active ingredient exhibits the inhibitory effect of NO generation, and an anti-inflammatory or trouble-improving composition using it was completed.

Korean Patent Registration No. 889,606

It is an object of the present invention to provide a cosmetic composition for improving anti-inflammatory or trouble, characterized in that it comprises one or more extracts selected from the group consisting of ginseng, maekmundong, rhubarb and bokryeong or fractions thereof as an active ingredient.

In order to solve the above problems, as an embodiment of the present invention, anti-inflammatory or trouble improving, characterized in that it comprises one or more extracts selected from the group consisting of ginseng, lichen, rhizome, and bokryeong or a fraction thereof as an active ingredient It provides a cosmetic composition.

In another embodiment of the present invention, it provides a cosmetic composition for improving anti-inflammatory or trouble, characterized in that it comprises a mixed extract of ginseng, malicmundong, rhubarb and bokryeong or a fraction thereof as an active ingredient.

In another embodiment of the present invention, the mixing ratio of the ginseng, malicmundong, rhubarb and bokryeong may be 25 to 35: 25 to 35: 25 to 35: 1 to 15 by weight, and specifically the ginseng, malicmundong, The mixing ratio of Rehmannia and Bokryeong may be 30:30:30:10 by weight.

In another embodiment of the present invention, the mixing ratio of the ginseng, malimundong, rhizome and bokryeong may be 30 to 35: 30 to 35: 28 to 32: 1 to 5 by weight, specifically the ginseng, malicmundong, The mixing ratio of Rehmannia and Bokryeong may be 34:34:30:2 by weight.

In the present invention, ginseng ( Ginseng Radix ) means the root of a perennial plant belonging to the genus Araliaceae (Panax). Specifically, ginseng may be dried. Ginseng is a representative medicinal plant and has a very slow growth rate, so it takes 4-6 years of cultivation to obtain sufficient medicinal ingredients. The main active ingredient of ginseng is known as saponin or ginsenosides, a complex carbohydrate (alcohol or a complex of phenol and sugar). These components excite and sedate the central nervous system, regulate metabolism, reduce blood sugar, improve muscle activity, excite the endocrine system, and maintain the concentration of hormones appropriately. In particular, saponins of ginseng are mostly triterpenoid dammaran saponins, and are unique saponins that exist only in plants of the genus Ginseng. Plant saponin has a hemolytic action, and in particular, the saponin component of ginseng is a neutral glycoside with little toxicity.

In the present invention, the Liliaceae (Liliaceae) is a perennial plant belonging to the Liliaceae Liliaceae (Ophiopogon) japonicus ) means the tuber. Specifically, dry ones can be used for lichens. Liqueuritis is particularly good for diabetes and is used as a diuretic, antibacterial, antipyretic, tonic, antitussive expectorant and cardiac.

In the present invention, Rehmannia is a perennial plant belonging to the Hyeonsam family ( Scrophularaceae), Rehmannia glutinosa ) means the root. Raw material is called Saengjihwang, dried one is called Geonjihwang, and steamed and dried is called Sukjihwang. Regardless of the above form, all can be used, but specifically, dried ones can be used. Rehmannia is used for beonjo, thirst, osteopathy, small thirst, hematopoiesis, nosebleeds, and can treat skin rashes caused by heat, and pharmacological effects such as lowering blood sugar, strong heart, diuresis, and antibacterial activity have been reported.

In the present invention, bokryeong refers to the scleroculus of bokryeong ( Poria cocos ) belonging to the Polyporaceae family. Specifically, dry bokryeong may be used. Bokryeong can not urinate, swelling of the stomach and whole body, and when there is seawater, vomiting, diarrhea due to drinking, forgetfulness due to nervousness, oil well, and can also be used for heart edema. In addition, pharmacological effects such as diuresis, hypoglycemic action, immunity enhancing action, and anti-tumor action have been reported.

As used in the present invention, the term "extract" refers to an extract obtained by extraction treatment of the ginseng, lichen, rhizome, bokryeong, or a mixture thereof, a dilution or pesticide solution of the extract, a dried product obtained by drying the extract, and the extract. It includes the extract of all formulations that can be formed using the extract itself and the extract, such as a crude product, a purified product, or a mixture thereof.

The extract of the present invention may be extracted from natural, hybrid or variant plants of each of the corresponding plants, and may be extracted from plant tissue cultures.

In the extraction of the ginseng, lichen, rhizome, bokryeong, or a mixture thereof of the present invention, the method of extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, and these may be performed alone or in combination of two or more methods.

In the present invention, the type of the extraction solvent used for extracting the ginseng, lichen, rhizome, bokryeong, or a mixture thereof is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; _{C 1} to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. In the present invention, more preferably, water (or distilled water) may be used as a solvent for extracting the herbal medicinal herbs of ginseng, maekmundong, rhizome, and bokryeong. A solvent extract can be prepared by extracting the herbal medicinal herbs of ginseng, Macmundong, Jihwang, and Bokryeong using the solvent at least once, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray drying can be prepared. . In a specific embodiment of the present invention, water (or distilled water) was used to prepare an extract of ginseng, malicmundong, rhizome, bokryeong, or a mixture thereof (Examples 1 to 6).

In the present invention, the extract extracted with hot water or cold needle is filtered to remove suspended solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., or use as such, or freeze drying, hot air drying, It can be used after drying by spray drying or the like.

As used herein, the term "fraction" means a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing various various constituents.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. A non-limiting example of the fractionation method may include a method of obtaining a fraction from the extract by treating an extract obtained by extracting ginseng, lichen mulberry, rhizome, bokryeong, or a mixture thereof with a predetermined solvent.

In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; Nonpolar solvents, such as hexane (Hexane), ethyl acetate (Ethyl acetate), chloroform (Chloroform), and dichloromethane, etc. are mentioned. These may be used alone or in combination of two or more. In the case of using an alcohol in the fractionation solvent, specifically, _{an alcohol of C 1} to C ₄ may be used.

The ginseng extract, licorice extract, rhubarb extract, bokryeong extract, or a mixture extract thereof of the present invention effectively inhibits the production of nitrogen monoxide (N0), thereby exhibiting anti-inflammatory and trouble-improving efficacy. In addition, since the composition of the present invention is a material derived from a natural product, it is not toxic, and even when applied to the human body, side effects are not caused, so it can be used as a cosmetic composition.

Inflammatory reaction is a mechanism to regenerate damage caused by physical action, harmful substances, chemical stimulation, bacterial infection, etc. as part of the defense response of living tissues against external stimuli. The persistent inflammatory reaction rather causes damage to the mucous membrane and promotes damage, inflammation and skin troubles in the skin tissue.

Macrophages play a very important role in the immune response and regulate a variety of inflammatory mediators, including nitric oxide, prostaglandins, and the immune protein inflammatory cytokines. Among them, nitrogen monoxide plays an important role, such as killing bacteria or removing tumors, but excessive production of nitrogen monoxide caused by pathogenesis causes inflammation, and such inflammation causes skin troubles.

That is, when the production of nitrogen monoxide by macrophages is suppressed, skin inflammation can be prevented, alleviated, improved, and treated, and skin troubles caused by an inflammatory reaction can be prevented, alleviated, improved, and treated.

Specifically, in one embodiment of the present invention, it was confirmed that when using ginseng extract, licorice extract, rhubarb extract, bokryeong extract, or a mixture extract thereof, respectively, the production of nitrogen monoxide in macrophages was suppressed compared to the use of the basic medium (Example 7).

In particular, the extract of the mixture containing all of ginseng, lichen, rhizome, and bokryeong of the present invention or a fraction thereof is a synergistic effect or cooperative action between the extracts and/or the active ingredients in the extract to the single extract of each plant. Compared to L-NMMA (L-NG-Monomethylarginine), which is a known nitric oxide production inhibitor, considering that it has a better ability to inhibit nitrogen monoxide production and is not purified at all, it produces very strong nitrogen monoxide in macrophages equal to or higher than that of L-NMMA (L-NG-Monomethylarginine). It was confirmed that there is an inhibitory ability, which was first identified in the present invention.

Specifically, the extract of the mixture containing both ginseng, lichen, rhizome and bokryeong of the present invention or a fraction thereof further increases the ability to inhibit nitric oxide production compared to the sole extract of ginseng, lichen, rhizome or bokryeong, resulting in more excellent anti-inflammatory and It can show the effect of improving skin troubles.

In the present invention, the mixing ratio of ginseng, lichen, rhizome and bokryeong may be specifically 25 to 35: 25 to 35: 25 to 35: 1 to 15 by weight, and more specifically 30 to 35: 30 to 35: 28 to 32: may be 1 to 5.

According to a specific embodiment of the present invention, a mixed extract having a mixing ratio of 30:30:30:10 and a mixed extract of 34:34:30:2 is prepared to produce nitrogen monoxide thereof. As a result of investigating the degree of inhibition, it was confirmed that the degree of inhibition of nitrogen monoxide production was greater than that of the single extract of ginseng, malicmundong, rhubarb or bokryeong (Example 7).

As used herein, the term "anti-inflammatory" refers to any action in which the immune response is alleviated and NO production is suppressed.

As used herein, the term "trouble" refers to skin conditions such as skin rash, acne, pimples, rosacea (red nose) that may be caused by inflammation caused by excessive nitric oxide production in macrophages.

As used herein, the term "trouble improvement" refers to suppressing or inhibiting the generation of troubles in the skin, or alleviating the troubles that have already been generated.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

The cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream, or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but the present invention is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as a carrier component. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

In the cosmetic composition of the present invention, the herbal extracts of ginseng, licorice mums, ginseng, bokryeong, or mixtures thereof may be included in a dry weight, 0.0001 to 50% by weight of the total weight of the cosmetic composition, and more specifically 0.0005 to 10 It is preferably included in weight percent. There is an advantage of showing excellent anti-inflammatory or trouble-improving efficacy within the above range, and there is an advantage of stabilizing the formulation of the composition.

The cosmetic composition comprising the ginseng, maekmundong, rhizome, bokryeong, and mixed extracts or fractions thereof of the present invention as an active ingredient is excellent in anti-inflammatory or skin trouble improvement effect. Specifically, the composition of the present invention is excellent in inhibiting the production of nitrogen monoxide in macrophages.

In addition, since the composition of the present invention is a material derived from a natural product, it is not toxic, and even when applied to the human body, it does not cause side effects, so its utilization as a cosmetic composition is high.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

Example 1. Preparation of ginseng extract

After drying and mincing ginseng, 100 g of dry weight was put into a flask, and extracted by cold soaking with 1,000 g of an extraction solvent (distilled water) for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 μm to prepare a ginseng extract.

Example 2. Of licorice extract Produce

After drying and slicing the licorice, 100 g of dry weight was put into a flask, and extracted by cold soaking with 1,000 g of an extraction solvent (distilled water) for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 μm to prepare a licorice extract.

Example 3. Rehmannia extract Produce

After drying and crushing the Rehmannia, 100 g of dry weight was put into a flask, and extracted by cold soaking with 1,000 g of an extraction solvent (distilled water) for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 μm to prepare a Rehmannia extract.

Example 4. Bokryeong extract Produce

After drying and slicing bokryeong, 100 g of dry weight was put into a flask, and extracted by cold soaking with 1,000 g of an extraction solvent (distilled water) for 3 days. Bokryeong extract was prepared by filtering the cold-precipitated extract through a filter having a pore size of 0.2 μm.

Example 5. Preparation of Mixed Extract 1 of Ginseng, Macmundong, Rehmannia and Bokryeong

After drying and mincing ginseng, maekmundong, rhizome, and bokryeong, 100 g of dry weight (30 g of ginseng, 30 g of maekmundong, 30 g of rhubarb, and 10 g of bokryeong) was placed in a flask, and extracted by cold simmering with 1,000 g of extraction solvent (distilled water) for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 µm to prepare a mixed extract 1 of ginseng, maekmundong, rhubarb, and bokryeong.

Example 6. Manufacture of mixed extract 2 of ginseng, maekmundong, jihwang and bokryeong

After drying and mincing ginseng, Maekmundong, Rehmannia and Bokryeong, 100g of dry weight (34g of Ginseng, 34g of Maekmundong, 30g of Rehmannia and 2g of Bokryeong) was put in a flask, and extracted by cold-sinking with 1,000g of extraction solvent (distilled water) for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 µm to prepare a mixed extract 2 of ginseng, maekmundong, rhubarb, and bokryeong.

Example 7. NO Investigation of anti-inflammatory effect by production inhibition

In order to confirm the anti-inflammatory effect and skin trouble alleviation effect of the herbal extracts of Examples 1 to 6, nitric oxide (NO) formation inhibition was conducted by the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278). I did.

Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, placed in a 24-well plate so as to enter each ^{of 3×10 5 cells in each well, and cultured for 24 hours.} Subsequently, the herbal extracts prepared in Examples 1 to 6 at the concentrations shown in Table 1 below were diluted and replaced with a cell medium containing. At this time, the NO-production inhibitor L-NMMA (L-NG-Monomethylarginine) was treated together as a positive control and incubated for 30 minutes, and LPS (Lipopolysaccharide) was treated as a stimulator by 1 µg and cultured for 24 hours. 100 µl of the supernatant was taken and transferred to a 96-well plate, 100 µl of the GRIESS solution was added at a time, reacted for 10 minutes at room temperature, and the absorbance at 540 nm was measured to determine the NO inhibitory effect of the herbal extract, and the results are shown in the table below. It is shown in 1. In addition, the experiment was repeated 3 times.

sample(%) NO production inhibition rate (%) Negative control - L-NMMA (10㎍/ml) 38.21 Example 1 (0.1%) 13.46 Example 1 (1.0%) 26.51 Example 2 (0.1%) 10.97 Example 2 (1.0%) 28.47 Example 3 (0.1%) 11.93 Example 3 (1.0%) 21.20 Example 4 (0.1%) 5.32 Example 4 (1.0%) 13.57 Example 5 (1.0%) 35.20 Example 6 (1.0%) 39.52

As can be seen from the results of Table 1, ginseng extract, licorice extract, rhizome extract, bokryeong extract, and mixed extracts of these herbal medicines have the effect of inhibiting NO production, and when extracted by mixing 4 herbal medicines, each herbal medicine alone extract Compared to that, it was found that there is an effect of suppressing the generation of NO more significantly. In particular, it was confirmed that when extracting the 4 herbal medicinal herbs in a mixture of 34:34:34:2 by weight, it exhibited excellent NO production inhibitory efficacy similar to that of L-NMMA, which is known as an NO production inhibitor.

Manufacturing example 1. Preparation of essence containing the mixed extract of the present invention

A cosmetic composition of an essence formulation was prepared as shown in Table 2 below (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Example 6 extract 5 Purified water To 100

Manufacturing example 2. Preparation of a cream containing the mixed extract of the present invention

As shown in Table 3 below, a cream-formed cosmetic composition was prepared (unit:% by weight).

Composition Content (% by weight) Example 6 extract 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Appropriate amount incense Appropriate amount Purified water to 100

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed as including all changes or modified forms derived from the meaning and scope of the claims to be described later rather than the above detailed description and equivalent concepts thereof.

Claims (8)

It contains a mixed extract of ginseng, malimundong, rhododendron and bokryeong or a fraction thereof as an active ingredient, and the mixed ratio of ginseng, malimundong, rhubarb and bokryeong is 34: 34: 30: 2 by weight. Cosmetic composition for improvement.
delete delete delete delete delete The method of claim 1, wherein the extract is obtained by extraction with at least one solvent selected from the group consisting _{of water, C 1} to C _{4 lower alcohol, 1,3-butylene glycol, and ethyl acetate.} Cosmetic composition for improving anti-inflammatory or trouble.
The cosmetic composition of claim 1, wherein the anti-inflammatory or trouble-improving is due to suppression of nitrogen monoxide (NO) production.