KR20190048662A - Cosmetic composition containing Chenopodium album extract and Dioscorea japonica Thunb. extract, and preparation method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Chenopodium album extract and a Dioscorea japonica extract, and a manufacturing method thereof. It is possible to provide a cosmetic composition which can be used for at least one selected from the group consisting of anti-oxidant and anti-inflammatory activities by comprising a mixed extract of a Chenopodium album extract and a Dioscorea japonica extract as an active ingredient.

Description

(Cosmetic composition containing Chenopodium album extract and Dioscorea japonica Thunb. extract, and preparation method thereof}

The present invention relates to a cosmetic composition for antioxidant and / or antiinflammatory comprising a sweetpotato extract and a yam extract, and a process for producing the same.

Due to recent stress and obesity due to Westernized eating habits, the incidence of inflammation related diseases in modern people is increasing. As a result, serious side effects such as gastric, renal, cardiac and vascular dysfunctions have been discovered upon long-term administration of chemically synthesized anti-inflammatory drugs, and efforts to develop anti-inflammatory drugs from natural sources with relatively low side effects have been continued .

In addition, natural product preparations with antioxidant ability mainly contain phenolic acid and flavonoid, and drugs and medicines having many antioxidant properties have excellent anti-inflammatory effect, Are being actively carried out.

Inflammation is an innate immunity that is a homeostatic response that protects and maintains our bodies from infection by viruses or bacteria. However, excessive acute inflammation and chronic inflammation are known to be the main causes of cancer, diabetes and vascular diseases, the three major causes of death. Specifically, metabolic diseases, vascular-related diseases, autoimmune diseases, neurological diseases, musculoskeletal diseases, and cancers are caused by various pathological processes (radical generation, inflammatory cytokine generation, chemokine generation, etc.) Is known to occur. Thus, if the inflammatory response can be appropriately and effectively controlled and treated, it is believed that the major cause of death can be sufficiently reduced. Thus, there is a need for a substance that can safely and effectively inhibit the inflammatory response.

On the other hand, anti-inflammatory and antioxidative cosmetics are generally used for cosmetics, base, point, make-up cosmetics for nail care, body cosmetics such as cleansing, sun care, sun tanning, shampoo- And cosmetic preparations that can be used for hair such as hair, hair, skin lotion, shampoo, rinse, treatment, wax, spray, mousse, hair lotion, essence, hair cream, permanent hair dye, temporary hair dye,

However, such anti-inflammatory and antioxidant cosmetic materials and medicinal ingredients have a problem in that they do not show a great effect after being treated when they are used for skin.

Accordingly, a cosmetic composition which is excellent in anti-inflammatory and antioxidative effects and harmless to the human body is required.

Korea Patent Publication No. 2017-0114796 Korea Patent Publication No. 2017-0114539

It is an object of the present invention to provide a cosmetic composition which can be used in at least one selected from the group consisting of antioxidant and anti-inflammatory, including a sweet pea extract and a yam extract.

Another object of the present invention is to provide a method for producing the cosmetic composition.

To achieve the above object, the cosmetic composition of the present invention may contain, as an active ingredient, a mixed extract obtained by mixing a K. juju extract and a Yams extract.

The koji extract and yam extract can be mixed at a weight ratio of 1: 1-15.

The Corynebuga alba extract may be extracted by mixing Corynebia radix with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof in a weight ratio of 1:20 to 50.

The yamma extract may be extracted by mixing yam and water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof in a weight ratio of 1:20 to 50.

The mixed solvent may be 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol.

The honeysuckle extract and yam extract can be obtained by extracting at 20 to 35 DEG C for 40 to 55 hours.

The cosmetic composition may be at least one selected from the group consisting of antioxidants and anti-inflammatory agents.

The cosmetic composition may be at least one selected from the group consisting of skin, lotion, essence, lotion, essence, body lotion, bar diesel, body essence, body cleanser, cleansing foam, cleansing cream, cleansing gel, pack, massage cream, massage gel, , And the like.

The cosmetic composition may further comprise at least one member selected from the group consisting of water, oil, a surfactant, a moisturizer, a thickener, a fragrance, a preservative, a neutralizer, an emollient, a skin protection agent and a skin conditioner.

In another aspect of the present invention, there is provided a method for preparing a cosmetic composition, comprising the steps of (A) mixing starch and a solvent in a weight ratio of 1:20 to 50 and extracting the mixture at 20 to 35 ° C for 40 to 55 hours, Obtaining an extract; (B) mixing yam and solvent at a weight ratio of 1:20 to 50 and extracting at 20 to 35 ° C for 40 to 55 hours to obtain a yams extract; And (C) mixing the sweet pea extract and the yamma extract.

The koji extract and yam extract can be mixed at a weight ratio of 1: 1-15.

The solvent may be 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol.

The cosmetic composition comprising the mixed extract of the present invention and the yam extract as an active ingredient provides a semi-permanent antioxidant and anti-inflammatory effect without damaging the skin.

In addition, the cosmetic composition of the present invention is a cosmetic composition derived from a natural product which is naturally friendly and has few side effects, and thus has an advantage of satisfying the demand of consumers.

FIG. 1 is a graph showing the DPPH radical scavenging activity measured according to the concentrations of K. juice extract and Yams extract.
FIG. 2A is a graph showing IL-6 production of extracts prepared according to Examples and Comparative Examples for LPS-activated brain cells. FIG.
FIG. 2B is a graph showing IL-6 production of extracts prepared according to Examples and Comparative Examples for LPS-activated splenocytes. FIG.
FIG. 2C is a graph showing IL-6 production of extracts prepared according to Examples and Comparative Examples for LPS-activated colon cells. FIG.
FIG. 3A is a graph showing TNF-.alpha. Production of extracts prepared according to Examples and Comparative Examples for LPS-activated brain cells. FIG.
Figure 2B is a graph showing TNF-a production of extracts prepared according to Examples and Comparative Examples for splenocytes activated with LPS.
FIG. 2C is a graph showing TNF-.alpha. Production of extracts prepared according to Examples and Comparative Examples for LPS-activated colon cells. FIG.

The present invention relates to a cosmetic composition which can be used in at least one selected from the group consisting of antioxidative and anti-inflammatory agents, including a sweet pea extract and a yam extract, and a process for producing the same.

Hereinafter, the present invention will be described in detail.

The cosmetic composition of the present invention contains, as an active ingredient, a mixed extract obtained by mixing a K. juju extract and a Yams extract.

Chenopodium album var. Centrorubrum (C. album) has its origin in southern China, and about 1,500 species are distributed worldwide, and 15 species are known to be distributed in Korea. The baboon is a one-year-old herb that is commonly found in disturbed, scattered and dried habitats throughout the country. It has been used as a medicinal product in Korea because it has been effective in preventing hypertension, intracerebral hemorrhage and paralysis. It has been used as a medicinal product to dry the leaves. It has been used for pain relief such as toothache and sore throat, It was used variously until insect bite. It is known that honey is rich in nutrients such as dietary fiber, magnesium and vitamin K. It is also known that it contains nutritional components such as dietary fiber, magnesium and vitamin K. In addition, it contains amino acids such as β-carotene, calcium, iron, essential fatty acid, sitosterol, leucin, betain, A, B1, B2, C, and the like. Antibiotics, antivirals, anticancer effects, and anti-diabetic effects have been studied.

In addition, the yam (Dioscorea japonica Thunb) is a perennial herbaceous perennial belonging to the family Dioscoreaceae and has thick roots in the ground. It is native to China and is native to all parts of the country and found in bushes and grasses, and is widely cultivated mainly in the Jeonbong Mountain region. Yam is a pharmacological ingredient contained in rhizomes and contains batasin, mucin, amylase, mucilage, and allantoin, and has been used in the treatment of intestinal tract, pulmonary tuberculosis and diabetes. In addition, saponin contained in yams is known to reduce arteriosclerosis, hypertension and blood cholesterol content. Of these, dioscin and diosgenin, which are steroidal saponins, have pharmacological effects such as antibacterial, antifungal, hypoglycemic, digestive, . In addition, it has been studied that the EtOAc (ethyl acetate) fraction of yam inhibits the growth of human lung cancer cell lines. Experiments on the function and lipid metabolism of yam in Balb / c mice have revealed that leucine aminopeptidase activity Decreased cholesterol, decreased LDL (low density lipoprotein cholesterol) and decreased fat absorption.

The Corynebugi extract and Yams extract are mixed at a weight ratio of 1: 1-15, preferably 1: 1-10, more preferably 1: 2-3. When the content of yam extract is lower than the lower limit based on honey extract, there may be little effect of antioxidation and anti-inflammation. If the content is above the upper limit, skin trouble may occur.

In order to prepare the koji extract and the yam extract, firstly the lobster and yam are respectively washed with water and dried.

The washed starch and the extraction solvent are mixed at a weight ratio of 1:20 to 50, preferably 1:25 to 40, at 20 to 35 ° C, preferably 23 to 27 ° C for 40 to 55 hours, And extracted for 45 to 50 hours to prepare an extract.

When the extraction temperature and time are less than the lower limit, the antioxidant and anti-inflammatory effects may hardly occur. If the extraction temperature and time are above the lower limit, the antioxidant and anti-inflammatory effects may be lowered.

The extraction solvent is water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The mixed solvent is not particularly limited, but an aqueous solution of methanol, ethanol, butanol or propanol in an amount of 20 to 80% Is a 20 to 80% aqueous solution of ethanol.

When the weight ratio of yam and yam to the extraction solvent is out of the range, the extract of yam and yam can be extracted in a small amount.

The jujuba extract and yam extract are each contained in an amount of 0.1 to 3% by weight, preferably 0.2 to 2% by weight.

If the content of each of K. mori extract and yam extract is out of the above range, resistance against oxidation and inflammation may not be excellent.

The koja extract and the yamma extract prepared as described above may be used as the extract itself or may be pulverized for convenient use. The method of pulverizing the koji extract and the yamma extract is as follows. The extract is concentrated under reduced pressure to reduce its volume, and then lyophilized by a freeze dryer to be pulverized.

The present invention also provides a method for preparing a cosmetic composition.

The method for producing a cosmetic composition of the present invention comprises the steps of: (A) mixing honey and a solvent at a weight ratio of 1:20 to 50 and extracting at 20 to 35 ° C for 40 to 55 hours to obtain a honey extract; (B) mixing yam and solvent at a weight ratio of 1:20 to 50 and extracting at 20 to 35 ° C for 40 to 55 hours to obtain a yams extract; And (C) mixing the sweet pea extract and the yamma extract.

First, in step (A) and step (B), starch and yam are respectively mixed with a solvent and extracted at 20 to 35 ° C for 40 to 55 hours to thereby obtain a honey extract and a yam extract.

Next, in step (B), the jujuba extract and the yamma extract are mixed at a weight ratio of 1: 1-15, preferably 1: 1-10.

In the cosmetic composition of the present invention, other ingredients may be blended with the above-mentioned cosmetic composition, if necessary, usually in cosmetics. Examples of such ingredients include water, oil, surfactant, moisturizer, thickener, perfume, preservative, Emollients, skin protectants, and skin conditioners.

The cosmetic composition may be in the form of an emulsion or solubilized formulation commonly used in the art. Examples of the cosmetic composition include a skin, an emulsion, an essence, lotion, essence, body lotion, bar diesel, body essence, body cleanser, A foam, a foam, a cleansing cream, a cleansing gel, a pack, a massage cream, a massage gel, a nutritional cream, a sleep cream and a moisture cream.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Production Example 1. Preparation of sweet almond extract < RTI ID = 0.0 > 70% <

The sample was washed with 70% ethanol at a weight ratio of 1: 30 and extracted for 48 hours at room temperature. The filtrate was filtered twice with a filter paper and concentrated under reduced pressure to obtain lyophilized powder ≪ / RTI >

Production Example 2. Preparation of yam extract

Yams collected from Gurye - gun, Jeollabuk - do Province were washed and dried, and 70% ethanol was mixed at a weight ratio of 1: 30. The mixture was extracted at room temperature for 48 hours. The filtrate was filtered twice with a filter paper and concentrated under reduced pressure. ≪ / RTI >

Production Example 3. Production of sweet pea extract _ Water extraction

The same procedure as in Preparation Example 1 was carried out except that water was used instead of 70% ethanol as an extraction solvent to obtain a K. podis extract powder.

Production Example 4. Production of yam extract _ Water extraction

The same procedure as in Preparation Example 2 was carried out except that yam extract powder was obtained by using water instead of 70% ethanol as an extraction solvent.

Example 1 Starch Extract : Yams Extract = 1: 1

The honey extract prepared in Preparation Example 1 and the yam extract prepared in Preparation Example 2 were mixed at a weight ratio of 1: 1 to prepare a mixed extract.

Example 2 Sweet pea extract : yam extract = 1: 2

The same procedure was carried out as in Example 1, except that the honey extract and the yam extract were mixed at a weight ratio of 1: 2 to prepare a mixed extract.

Example 3 Sweet pea extract : yam extract = 1: 5

The same procedure as in Example 1 was carried out except that a honey extract and a yam extract were mixed at a weight ratio of 1: 5 to prepare a mixed extract.

Example 4. Sweet pea extract : yam extract = 1: 10

The same procedure as in Example 1 was carried out except that Sunflower extract and Yams extract were mixed at a weight ratio of 1:10 to prepare a mixed extract.

Example 5 Starch Extract : Yams Extract = 1: 15

The same procedure as in Example 1 was carried out except that the honey extract and yam extract were mixed at a weight ratio of 1:15 to prepare a mixed extract.

Comparative Example 1 Sweet pea extract alone

The koji extract prepared in Preparative Example 1 was used alone to prepare the K. rubra extract.

Comparative Example 2 Yam extract alone

The yam extract prepared in Preparation Example 2 was used alone to prepare a yam extract.

COMPARATIVE EXAMPLE 3 Aqua extract : yam water extract = 1: 2

The water extract of Q. japonica prepared in Preparation Example 3 and the yam water extract prepared in Preparation Example 4 were mixed at a weight ratio of 1: 2 to prepare a mixed extract.

<Test Example>

Test Example 1. Measurement of DPPH radical scavenging ability

Electron donating ability was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method to analyze the antioxidative activity of the mixed extracts prepared according to the above Examples and Comparative Examples. 1: 1, 1: 2, 1: 3, 1: 1, 1: 2, 1: 2 and 1: 1, respectively) were prepared by dissolving yam and extracts in ethanol (SAMCHUN Chemical, GYEONGGI, KOREA) (SIGMA, St. Louis, USA) dissolved in 70% ethanol and 100 μl of each sample were dispensed into a 96-well plate at a weight ratio of 1: 5, 1: 10 and 1:15 Were incubated at room temperature for 20 min and absorbance was measured at 520 nm using a microplate reader (Molecular Devices, Sunnyvale, Calif., USA).

Free radical scavenging activity was expressed as percentage compared to negative control without sample. As a positive control, ascorbic acid, a representative antioxidant, was used. The percentage formula of electron donating ability is expressed by the following formula (1).

The DPPH radical scavenging activity is measured using the principle that DPPH is a stable free radical of a purple color and is reduced by the antioxidant substance and the deep purple is discolored. The measurement method is relatively simple and is useful for searching the antioxidant activity from various samples.

[Equation 1]

Electron donating ability (%) = {1 - (A - B) / C} 100

A: sample absorbance 520 nm, B: color control absorbance 520 nm (without DPPH), C: negative control absorbance 520 nm (without sample)

FIG. 1 is a graph showing the DPPH radical scavenging activity measured according to the concentrations of K. juice extract and Yams extract.

As shown in FIG. 1, the yam extract had an elimination activity of 18.01, 47.68, 66.04, 79.87, 84.76, 88.26, 89.05 and 89.64% at 0.1, 0.5, 1, 2, 4, 6, 8 and 10 mg / Respectively; It was confirmed that the extracts of Kojooju showed 10.25, 14.02, 22.24, 41.92, 62.71, 71.96, 75.07, and 77.73% scavenging activity. However, since both the yam and extract extracts showed excellent DPPH radical scavenging ability at 10 mg / ml, it was found that the extracts of Yams and Kwangju extracts showed the highest DPPH radical scavenging ability at 10 mg / ml Respectively.

Table 1 below shows the DPPH radical scavenging ability (percentage of electron donating ability) of the mixed extract prepared according to the above Examples and Comparative Examples.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 DPPH radical scavenging ability 91.44 93.16 90.86 90.81 81.06 89.64 77.73 70.41

As shown in Table 1 above, it was confirmed that the mixed extracts prepared according to Examples 1 to 5 of the present invention had better DPPH radical scavenging ability than Comparative Examples 1 to 3.

In particular, it was confirmed that Example 2 exhibited far superior DPPH radical scavenging ability than Example 1 and Examples 3 to 5.

Test Example 2. Measurement of Total Polyphenol Content _ Antioxidant

To determine the total polyphenol content of the mixed extracts prepared according to the above Examples and Comparative Examples, the content was measured by Folin-Ciocalteu's coloring method. In other words, 1 mL of 0.2 N Folin-Ciocalteu's reagent was added to 200 μL of each extract sample, and the mixture was allowed to stand for 4 minutes. Then, 800 μL of 75 g / L Na ₂ CO ₃ solution was added thereto, The absorbance was measured. At this time, 0-500 mg / L gallic acid was used to prepare the standard curve, and the total polyphenol content was converted into gallic acid (mg GAE) / g.

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Chol Polyphenol Content (mg GAE / 100g) 36.11 46.58 38.14 36.84 31.49 17.47 35.15 18.11

As shown in Table 2 above, it was confirmed that the mixed extracts prepared according to Examples 1 to 5 of the present invention had a higher total polyphenol content than Comparative Examples 1 to 3.

In particular, it was confirmed that Example 2 exhibited a significantly higher total polyphenol content than Example 1 and Examples 3 to 5.

Test Example 3. Measurement of proinflammatory cytokine (IL-6, TNF-a) _ Anti-inflammatory

To investigate the effect of yam and extracts on the production of proinflammatory cytokines in primary culture cells induced by LPS, the levels of proinflammatory cytokines were measured by ELISA.

Cells from mouse brain, spleen, and large intestine were plated in a 24-well plate at 1 × 10 ⁶ cells / well and cultured in a 5% CO ₂ incubator for 2 hours. Cells were stimulated with LPS (1 μg / ml) At the same time, the extracts of Examples and Comparative Examples were treated and further cultured for 24 hours. The inflammatory mediators of proinflammatory cytokines were quantified by enzyme-linked immunosorbent assay (ELISA) using cell supernatants. ELISA kit was purchased from Youngin Frontier (CA, USA) and purchased Mouse ELISA kit for IL-6 and TNF-α according to the manufacturer's instructions. The brain is the most sensitive cell among the cells in our body. The spleen is a tissue sensitive to inflammatory tissues. The colon is a tissue having elasticity similar to skin. Therefore, if it shows efficacy in the brain, spleen and colon tissues, .

In the experiment, the 96-well plate coated with antibody was washed with washing buffer and blocked at room temperature for 1 hr. Then, 100 μl of the supernatant obtained by centrifuging the mucosal tissues of the colonic mucosa of each group three times, and then loaded on a 96-well plate, react with the capture antibody, IL-6 antibody, for 2 hr. The plate is washed again, bound to Biotin-coupled Detection Antibody, and incubated with Streptavidin-HRP (horseradish peroxidase) complex. TMB (Tetramethylbenzidine) in the substrate solution reacts with HRP, absorbance at 450 nm, and stops the reaction with Stop solution (2 NH ₂ SO ₄ ). The absorbance of the resulting solution was measured with a microplate reader, and the concentrations of IL-6 and TNF-α were measured using a standard curve.

IL-6

FIG. 2A is a graph showing IL-6 production of extracts prepared according to Examples and Comparative Examples for LPS-activated brain cells. FIG. 2B is a graph showing the activity of splenocytes activated by LPS according to Examples and Comparative Examples FIG. 2C is a graph showing IL-6 production of extracts prepared according to Examples and Comparative Examples for LPS-activated colon cells. FIG.

IL-6 is an immune system that induces acute-phase protein inflammation, which is produced by various hematopoietic and non-hematopoietic cells, including mononuclear cells, T cells and fibroblasts, in response to infection and tissue damage It is the main factor.

As shown in FIGS. 2A to 2C, the inhibitory effect of extracts of Examples and Comparative Examples on the brain, spleen, and colon cells of LPS-activated mice was inhibited by IL-6 production in brain, spleen, (1 mg / ml) of 1 to 5 was superior to the control (NOR) and Comparative Examples 1 to 3 in inhibiting IL-6 production.

TNF-a

FIG. 3A is a graph showing TNF-α production of LPS-activated brain cells according to Examples and Comparative Examples, and FIG. 2B is a graph showing the production of TNF-α by LPS-activated splenocytes according to Examples and Comparative Examples FIG. 2C is a graph showing TNF-.alpha. Production of extracts prepared according to Examples and Comparative Examples for LPS-activated colon cells. FIG.

Tumor necrosis factor-alpha (TNF-α, or TNF) is an inflammatory cytokine secreted mainly by activated macrophages and secreted by various cells such as T-cells, natural killer cells, and damaged neurons. TNF acts as a pyrogen in the body and has the ability to induce heat, to induce apoptosis, to induce sepsis through the production of IL-1 and IL-6, to induce infection, and to inhibit tumor formation and viral replication .

As shown in FIGS. 3A to 3C, the inhibitory effect of the extracts of Examples and Comparative Examples on the brain, spleen, and colon cells of LPS-activated mice was examined for inhibition of TNF- 1 to 5 (1 mg / ml) was superior to the control (NOR) and Comparative Examples 1 to 3 in inhibiting TNF-α production.

Test Example 4. Evaluation of cytotoxicity

The cells were cultured in Dulbecco's modified Eagle's medium containing 10% FBS and 15 P / S (50 units / mL penicillin, 25 mg / mL streptomycine) (DMEM; Gibco BRL, Grand Island, NY, USA) as a standard medium and cultured in a 75 cm ² culture flask while exchanging the medium every 2 days in a 5% CO ₂ incubator. The cells were then plated on trypsin / EDTA, plated on a 24-well plate at 1 × 10 ⁶ cells / well, incubated in a 5% CO ₂ incubator for one day, mg / mL, and then cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. The viability of the cells was measured by Crystal violet staining method. The experimental procedure was as follows: First, the media was removed from each well, washed with 1 ml of 1 × PBS three times, and then 10 μl of formalin solution was added to each well and fixed for 10 minutes. After fixing, 200 μl of 1% crystal violet prepared using 20% methanol was dispensed into each well, and stained at room temperature for 10 minutes. When the staining is complete, remove the staining solution from each well and wash with distilled water six times. The cell viability was calculated by measuring the absorbance of the stained cells at 595 nm by transferring a solution of 33% glacial acetic acid (250 μl) to each well and diluting 20 times.

&Quot; (2) &quot;

Cell viability (%) = (Test well / control well) x 100

division Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Cell survival rate (%) 135.2 138.5 131.4 132.8 135.2 78.1 126.4 130.4

As shown in Table 3, it was confirmed that the mixed extracts prepared according to Examples 1 to 5 of the present invention had better cell survival rates than those of Comparative Examples 1 to 3.

<Formulation example>

Hereinafter, formulation examples of the composition containing the mixture of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation Example 1. Preparation of cosmetic product of emulsified formulation

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared with the compositions shown in Table 4 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating to 65 to 70 占 폚.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 The cosmetic composition of Example 1 0.0001 One 10

Formulation Example 2. Preparation of Cosmetics of Solubilized Formulation

Cosmetic products of the solubilized formulations were prepared with the compositions shown in Table 5 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 The cosmetic composition of Example 1 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Claims (12)

A cosmetic composition characterized by containing as an active ingredient a mixed extract obtained by mixing a koji extract and a yam extract. 2. The cosmetic composition according to claim 1, wherein the honey extract and the yam extract are mixed at a weight ratio of 1: 1-15. [Claim 2] The cosmetic composition according to claim 1, wherein the honey extract is prepared by mixing Honeysuckle with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof at a weight ratio of 1:20 to 50. The cosmetic composition according to claim 1, wherein the yamma extract is prepared by mixing yam, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof at a weight ratio of 1:20 to 50. The cosmetic composition according to claim 3 or 4, wherein the mixed solvent is an aqueous solution of methanol, ethanol, butanol or propanol in an amount of 20 to 80% by volume. The cosmetic composition according to claim 1, wherein the extract is obtained by extracting at 20 to 35 DEG C for 40 to 55 hours. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of antioxidants and anti-inflammatory agents. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of a skin, an emulsion, an essence, lotion, essence, body lotion, bar diesel, body essence, body cleanser, cleansing foam, cleansing cream, cleansing gel, pack, massage cream, , A sleep cream, and a water cream. &Lt; RTI ID = 0.0 &gt; 11. &lt; / RTI &gt; The cosmetic composition according to claim 1, wherein the cosmetic composition further comprises at least one member selected from the group consisting of water, oil, surfactant, moisturizer, thickeners, fragrances, preservatives, neutralizing agents, emollients, skin protectants and skin conditioners . (A) mixing honey and solvent at a weight ratio of 1:20 to 50 and extracting at 20 to 35 ° C for 40 to 55 hours to obtain a honey extract;
(B) mixing yam and solvent at a weight ratio of 1:20 to 50 and extracting at 20 to 35 ° C for 40 to 55 hours to obtain a yams extract; And
(C) mixing the baby's sphincter extract and the yamma extract. [Claim 11] The method according to claim 10, wherein the sweet pea extract and yam extract are mixed at a weight ratio of 1: 1-15. 11. The method of claim 10, wherein the solvent is 20 to 80% by volume of an aqueous solution of methanol, ethanol, butanol or propanol.

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